Publication Date:
1989-04-28
Description:
Transcriptional activation of the human interleukin-2 (IL-2) gene, like induction of the IL-2 receptor alpha (IL-2R alpha) gene and the type 1 human immunodeficiency virus (HIV-1), is shown to be modulated by a kappa B-like enhancer element. Mutation of a kappa B core sequence identified in the IL-2 promoter (-206 to -195) partially inhibits both mitogen- and HTLV-I Tax-mediated activation of this transcription unit and blocks the specific binding of two inducible cellular factors. These kappa B-specific proteins (80 to 90 and 50 to 55 kilodaltons) similarly interact with the functional kappa B enhancer present in the IL-2R alpha promoter. These data suggest that these kappa B-specific proteins have a role in the coordinate regulation of this growth factor-growth factor receptor gene system that controls T cell proliferation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hoyos, B -- Ballard, D W -- Bohnlein, E -- Siekevitz, M -- Greene, W C -- A127053-01/PHS HHS/ -- New York, N.Y. -- Science. 1989 Apr 28;244(4903):457-60.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Mount Sinai Medical Center, Department of Microbiology, New York, NY 10029.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2497518" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Cell Line
;
Cloning, Molecular
;
DNA/metabolism
;
DNA-Binding Proteins/*metabolism
;
*Enhancer Elements, Genetic
;
*Gene Expression Regulation
;
Genes, Viral
;
HIV-1/genetics
;
HTLV-I Antigens/pharmacology
;
Humans
;
Immunoglobulin kappa-Chains/*genetics
;
Interleukin-2/*genetics
;
Molecular Weight
;
Mutation
;
Phytohemagglutinins/pharmacology
;
Plasmids
;
Promoter Regions, Genetic
;
RNA, Messenger/biosynthesis
;
T-Lymphocytes/metabolism
;
Tetradecanoylphorbol Acetate/pharmacology
;
Trans-Activators
;
Transcription Factors/pharmacology
;
Transcription, Genetic
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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