ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary The effect of the herbicides MCPA, MCPB, mecoprop, dichlorprop, 2,4-D, 2,4-DB, and 2,4,5-T on l-lysine fermentation was investigated using a lysine-producing mutant of Corynebacterium glutamicum. Stimulation of l-lysine production by 6% to 36% was observed in shaken flask experiments when the test herbicides were added at a concentration of 5 · 10-4 M to growing cultures after 24 h of cultivation. The most effective stimulators were MCPA, mecoprop and dichlorprop. Detailed studies of the effect of MCPA (5 · 10-6 M to 5 · 10-3 M) showed that the degree of stimulation depended on medium composition and aeration. In the synthetic medium, maximum production of 50 g · l-1 lys · HCl occurred at 5 · 10-4 M MCPA and an oxygen transfer rate (OTR) of 1.97 g O2 · l-1 · h-1, while 61.7 g · l-1 of lys · HCL was formed at 5 · 10-3 M MCPA and an OTR of 3.75 g O2 · l-1 · h-1. In the amino-nitrogen rich medium, maximum production of 42 g · l-1 lys · HCl was observed at 5 · 10-6 M MCPA and an oxygen transfer rate of 1.5 g O2 · l-1 · h-1. Results from batch l-lysine fermentation in a fermenter showed similar stimulatory effects, with an optimal concentration of MCPA for l-lysine production of 5 · 10-5 M. Without herbicide addition, the test strain produced 16.25 g · l-1 of product and with addition of 5 · 10-5 M MCPA, the same strain produced 52.1 g · l-1 lys · HCl after 72 h of fermentation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00252590
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