Summary
The antibiotic nikkomycin can be produced by calcium alginate immobilized mycelium of Streptomyces tendae Tü 901 in batch and continuous culture.
Scanning electron micrographs show the porous structure of the matrix and the distribution of the cells in the gel.
Some physiological properties of free and immobilized mycelia were compared. Immobilization does not change the relative amounts of nikkomycin compounds in the culture broth. DNA and protein content were the same in free and immobilized cells. The specific activity of fructosediphosphate aldolase dropped during fermentation and was lower for entrapped than for free cells. The specific activity of mannitol dehydrogenase increased up to the end of the fermentation and was the same for free and immobilized mycelium.
In continuous culture the relative amount of mannitol consumed decreased with increasing flow rate. When the medium was supplemented with amino acids mannitol consumption increased significantly.
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Dedicated to Prof. Dr. L. Acker on the occasion of his 70th birthday
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Veelken, M., Pape, H. Production of nikkomycin by immobilized Streptomyces cells — Physiological properties. Appl Microbiol Biotechnol 19, 146–152 (1984). https://doi.org/10.1007/BF00256445
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DOI: https://doi.org/10.1007/BF00256445