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  • 1
    Electronic Resource
    Electronic Resource
    Colorimetric determination of .gamma.-cyclodextrin (1984)
    s.l. : American Chemical Society
    Analytical chemistry 56 (1984), S. 1738-1740 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00273a049
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  • 2
    Electronic Resource
    Electronic Resource
    The cloned β-mannanase gene from alkalophilic Bacillus sp. AM-001 produces two β-mannanases in Escherichia coli (1989)
    Springer
    Archives of microbiology 152 (1989), S. 10-15 
    Details
    ISSN: 1432-072X
    Keywords: Alkalophilic Bacillus sp. ; β-Mannanase gene ; Escherichia coli ; Cloning ; Expression ; β-Mannanase ; Southern hybridization ; Western blotting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene encoding β-mannanase was cloned from alkalophilic Bacillus sp. AM-001 into Escherichia coli JM 101 by inserting HindIII-generated DNA fragments into the HindIII site of pUC19. A 2.0 kb XbaI-PstI fragment of the donor strain DNA was sufficient for β-mannanase synthesis. The amount of β-mannanase expressed in E. coli JM101 harboring pMAH3 (containing a 2.4 kb XbaI-HindIII fragment) was about 24% of the activity produced by the donor strain. E. coli JM101 harboring pMAH3 was found to produce two enzymatically active β-mannanases (A and B). These two β-mannanases were purified to electrophoretically homogenous states. The β-mannanase A had enzymatic properties similar to those of the β-mannanases M-I and M-II produced by alkalophilic Bacillus sp. AM-001, and the β-mannanase B resembled its β-mannanase M-III. In contrast to β-mannanase production in the donor strain, that in E. coli was not inducible. The NH2-terminal amino acid sequences from amino acid 1 (Asn) to 9 (Gln) of the three β-mannanases purified from alkalophilic Bacillus sp. AM-001 coincide with those from amino acid 4 (Asn) to 12 (Gln) of the two β-mannanases purified from E. coli transformant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447004
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  • 3
    Electronic Resource
    Electronic Resource
    Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. (1998)
    Springer
    Archives of microbiology 170 (1998), S. 331-338 
    Details
    ISSN: 1432-072X
    Keywords: Key words Barophilic bacteria ; Deep-sea ; Hydrostatic ; pressure ; Shewanella violacea ; Ryukyu Trench
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several barophilic Shewanella species have been isolated from deep-sea sediments at depths of 2,485– 6,499 m. From the results of taxonomic studies, all of these isolates have been identified as strains of Shewanella benthica except for strain DSS12. Strain DSS12 is a member of a novel, moderately barophilic Shewanella species isolated from the Ryukyu Trench at a depth of 5,110 m. On Marine Agar 2216 plates, this organism produced a violet pigment, whereas the colonies of other isolates (S. benthica) were rose-colored. Phylogenetic analysis based on 16 S ribosomal RNA gene sequences showed that strain DSS12 represents a separate lineage within the genus Shewanella that is closely related to S. benthica and particularly to the members of the Shewanella barophiles branch. The temperature range for growth and some of the biochemical characteristics indicate that strain DSS12 differs from other Shewanella species. Furthermore, strain DSS12 displayed a low level of DNA similarity to the Shewanella type strains. Based on these differences, it is proposed that strain DSS12 represents a new deep-sea Shewanella species. The name Shewanella violacea (JCM 10179) is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050650
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  • 4
    Electronic Resource
    Electronic Resource
    The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170 (1989)
    Springer
    Archives of microbiology 151 (1989), S. 91-94 
    Details
    ISSN: 1432-072X
    Keywords: Alkalophilic Bacillus ; Penicillinase ; β-Lactamase ; Class A enzyme ; Lipoprotein ; Amino acid homology ; Gene cloning ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lipo-penicillinase (LIPEN) gene from an alkalophilic Bacillus sp. strain 170 was cloned in Escherichia coli using the vector pHSG399. A plasmid, pFAP121, was isolated from an ampicillin resistant transformant and the cloned LIPEN gene was found to be in a 2.2 kb DNA fragment. The nucleotide sequence of a 1.9 kb segment encoding the LIPEN was determined. This segment showed an open reading frame which would encode a polypeptide of 310 amino acids. The amino acid sequence of this LIPEN gene product has strong homology with those of the Bacillus cereus β-lactamase III and Bacillus licheniformis penicillinase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414419
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  • 5
    Electronic Resource
    Electronic Resource
    Thermococcus peptonophilus sp. nov., a fast-growing, extremely thermophilic archaebacterium isolated from deep-sea hydrothermal vents (1995)
    Springer
    Archives of microbiology 164 (1995), S. 159-164 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Thermococcales ; Extreme thermophiles ; Archaea ; Deep-sea hydrothermal vents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two extremely thermophilic archaebacteria, strains OG-1 and SM-2, were isolated from newly discovered deep-sea hydrothermal vent areas in the western Pacific ocean. These strains were cocci, obligately anaerobic Archaea about 0.7–2 μm in diameter. Optimum growth conditions for OG-1 and SM-2 were at 85–90°C (range 60–100°C), pH 6 (range pH 4–8), a NaCl concentration of 3% (range 1–5%), and a nutrient concentration (tryptone plus yeast extract) of 0.2% (range 0.005–5%). Elemental sulfur stimulated the growth rate fourfold. Ammonium slightly stimulated growth. Both tryptone and yeast extract allowed growth as sole carbon sources; these isolates were not able to utilize or grow exclusively on sucrose, glucose, maltose, succinate, pyruvate, propionate, acetate, or free amino acids. OG-1 showed the fastest growth rate within the genus Thermococcus. Growth was inhibited by rifampicin. The DNA G+C content was 52 mol%. Sequencing of their 16S rDNA gene fragment indicated that these isolates belonged to the genus Thermococcus. OG-1 and SM-2 were different than the described Thermococcus species. We propose that OG-1 belongs to a new species: Thermococcus peptonophilus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050249
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  • 6
    Electronic Resource
    Electronic Resource
    Culturability and survival of an extreme thermophile isolated from deep-sea hydrothermal vents (1996)
    Springer
    Archives of microbiology 166 (1996), S. 64-67 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Deep-sea ; Hydrothermal vents ; Survival ; Extreme thermophiles ; Culturability ; Active cells ; Mortality ; Dead cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The culturability of a strictly anaerobic, extremely thermophilic archaeon, Thermococcus peptonophilus (optimal growth temperature: 85° C), was studied during survival stages at various temperatures (98, 85, 70, and 4° C). Total cell number (determined by DAPI staining), active cells (rhodamine-stained cells), and culturable cells (using most-probable-number) were counted over time. The number of culturable cells decreased under each condition tested. The total number of cells significantly decreased only at temperatures close to the maximum for growth (98° C); at this temperature, the cells spontaneously lysed. Our results suggested that survival at 4° C in oxygenated waters might be a mechanism for the dispersion of extreme thermophiles in the ocean. In addition, we proved the existence of T. peptonophilus cells in several physiological states: culturable cells, active non-culturable cells, inactive non-culturable cells, and dead cells. Cell death was caused by cellular lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050356
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  • 7
    Electronic Resource
    Electronic Resource
    Pressure and temperature effects on growth and viability of the hyperthermophilic archaeon Thermococcus peptonophilus (1997)
    Springer
    Archives of microbiology 168 (1997), S. 1-7 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Extreme thermophiles ; Large-scale cultivation ; Hydrostatic ; pressure ; Barophiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the effects of high temperatures and elevated hydrostatic pressures on the physiological behavior and viability of the extremely thermophilic deep-sea archaeon Thermococcus peptonophilus. Maximal growth rates were observed at 30 and 45 MPa although no significant increases in cell yields were detected. Growth at 60 MPa was slower. The optimal growth temperature shifted from 85° C at 30 MPa to 90–95° C at 45 MPa. Cell viability during the stationary phase was also enhanced under high pressure. A trend towards barophily at pressures greater than those encountered in situ at the sea floor was demonstrated at increasing growth temperatures. The viability of cells during starvation, at high temperature (90, 95° C), and at low temperature (10° C) was enhanced at 30 and 45 MPa as compared to atmospheric pressure. These results show that the extremely thermophilic archaeon T. peptonophilus is a barophile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050462
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  • 8
    Electronic Resource
    Electronic Resource
    Construction of a secretion vector production of peptide hormones in Escherichia coli (extracellular production of calcitonin as fused protein) (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 57 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A new secretion vector, pEAP84 which contained a unique restriction site (BglII) at the 3′ end of the penicillinase gene to produce a fused protein, and the Ex-kil region to make the outer membrane permeable, was constructed from pEAP82. A recombinant plasmid p84h06, which contained a synthetic gene for human calcitonin with a cyanogen bromide cleavage site at the junction site of the fused protein, was constructed and introduced into Escherichia coli. The hybrid protein produced in E. coli carrying p84h06 was secreted into the culture medium. The amino acid composition of this product was consistent with that deduced from the DNA sequence. Mature calcitonin was obtained following cyanogen bromide cleavage of the fused protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03360.x
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  • 9
    Electronic Resource
    Electronic Resource
    Isolation of strictly anaerobic halophiles from the aerobic surface sediments of hypersaline environments in California and Nevada (1989)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 57 (1989), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Four strictly anaerobic, chemoorganotrophic halophiles were isolated from the hypersaline surface sediments of the evaporating closed lagoon at the rim of Salton Sea, California, and of Big Soda Lake, Nevada, whose condition was not strictly anaerobic. All of the isolates were Gram-negative, motile, non-spore-forming, moderately halophilic eubacteria and required a minimum concentration of 3–10% NaCl in the growth medium. Among the four isolates, strain SS-21 could grow at more than 30% NaCl concentration, and strain M-20 was an alkalophine. Isolation of these bacteria suggests that a variety of anaerobic halophiles is widely distributed in hypersaline environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1989.tb03297.x
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  • 10
    Electronic Resource
    Electronic Resource
    High pressure represses expression of the malB operon in Escherichia coli (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 135 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The formation of plaques by A phage in Escherichia coli was prevented by elevated hydrostatic pressure; phage plaques were not detected at 30 MPa. Furthermore, using promoter fragments derived from the malB operon, we showed that gene expression initiated from both promoters (malK-lamB and malEFG) was repressed by elevated hydrostatic pressure. Our findings suggest that high pressure affects gene expression directed by the malB regulatory interval, and this may cause a decrease in the quantities of A receptor protein, LamB.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb07974.x
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