Summary
Final biomass yields of Chlorella vulgaris cultured heterotrophically in bristol medium amended with 0.1% (w/v) yeast extract (Difco) or 0.5% glucose (w/v) were 26 and 58 times higher, respectively, than yields obtained for autotrophically grown cells in the light. Similarly, final biomass increases were 35 and 138 fold for these organic substrates in the dark. The mixture of 0.1% yeast extract and 0.5% glucose was optimal and produced increases in final biomass of 70 and 140 times in the light and dark, respectively.
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Burrell, R.E., Inniss, W.E. & Mayfield, C.I. Development of an optimal heterotrophic growth medium for Chlorella vulgaris . Appl Microbiol Biotechnol 20, 281–283 (1984). https://doi.org/10.1007/BF00250641
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DOI: https://doi.org/10.1007/BF00250641