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  • Drosophila  (23)
  • reservoir  (23)
  • Springer  (46)
  • Annual Reviews
  • Blackwell Publishing Ltd
  • 2020-2024
  • 2005-2009
  • 1990-1994
  • 1980-1984  (46)
  • 1975-1979
  • 1982  (46)
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  • 2020-2024
  • 2005-2009
  • 1990-1994
  • 1980-1984  (46)
  • 1975-1979
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  • 1
    Electronic Resource
    Electronic Resource
    On the relation between genetic and environmental variability in animals (1982)
    Springer
    Journal of molecular evolution 18 (1982), S. 310-314 
    Details
    ISSN: 1432-1432
    Keywords: Neutral mutation theory ; Natural selection ; Protein evolution ; Levene model ; Environmental variability ; Genetic variability ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary If a phenotypic character is under stabilizing selection, the selective disadvantage of a nonoptimal genotype will decrease exponentially to zero as the proportion of phenotypic variation that is environmental in origin -V e /V p - increases. Under the modified mutation-drift hypothesis of genetic polymorphism, the proportion of mutations that are effectively neutral and average heterozygosity should increase with this ratio. Invertebrates, because of their small size, fast development, and low degree of homeostasis (relative to vertebrates), are expected to show a larger environmental component of phenotypic variation than vertebrates. This may help explain why invertebrates are in general more genetically variable than vertebrates and why, when laboratory populations ofDrosophila are maintained in heterogeneous environments, genetic variability is lost less rapidly than when they are kept in constant conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01733897
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  • 2
    Electronic Resource
    Electronic Resource
    Correlation between adult transformation and aldehyde oxidase staining pattern in wing discs ofApterous genotypes inDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 285-288 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal discs ; homoeosis ; Compartments ; Aldehyde oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The aldehyde oxidase staining pattern in wing discs ofDrosophila melanogaster bearing the genotypesap blt /ap blt andap blt andap blt /ap 73n showns changes from the wild-type pattern. Extensive areas of the presumptive dorsal posterior wing blade, which are normally unstained, have enzyme activity in these mutants. In wings of these genotypes, dorsal posterior structures are replaced by dorsal anterior wing structures. A strong correlation has been found between the frequencies of various staining patterns in the discs and the extent of transformation in the cuticular structures of the wing, which is consistent with the idea that aldehyde oxidase activity can be used as an indicator in the wing disc of this transformation. Unlike the homoeotic mutationengrailed, apterous has not been interpreted as a selector gene yet the work reported here shows thatapterous alleles can cause changes resembling those of theengrailed phenotype both in aldehyde oxidase staining behaviour and in the cuticular transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848418
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of the “sexcombless” chromosome (In(1)sx) on males and females ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 264-269 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Sexcombless ; Foreleg basitarsus ; Genital disc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromosome which carries the mutationsexcombless (In(1)sx) affects males and females ofD. melanogaster. In the male foreleg basitarsi the number of sexcomb teeth is dramatically reduced from 10 to 0.7 and the number of transverse rows of bristles is increased from 6 to 8. Females homozygous forIn(1)sx show a normal bristle pattern in the foreleg basitarsus. The genital disc derivatives of both male and femaleIn(1)sx flies are strongly affected. While the external genitalia show a duplicated or a reduced bristle pattern, the internal genitalia are mostly absent. However, the sexually dimorphic tergites and sternites of the abdomen remain unaffected. The male-specific effect on the basitarsus and the general effects on the genital disc derivatives are proposed to represent two different phenotypic effects ofIn(1)sx which may derive from mutations at different gene loci in the inverted chromosome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848414
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  • 4
    Electronic Resource
    Electronic Resource
    Growth is required for cell competition in the imaginal discs ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 289-291 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal discs ; Cell competition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Imaginal wing discs from late third-instar larvae were gammairradiated to induce clones of rapidly growingMinute − cells in a background of slowly growingMinute cells and culturedin vivo for periods up to 18 days. Clones in discs cultured for 16 to 18 days did not grow significantly larger than clones in uncultured controls, indicating that competition between populations of cells having potentially different mitotic rates does not occur in imaginal discs after their growth is completed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848419
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  • 5
    Electronic Resource
    Electronic Resource
    Sequential gene activation by ecdysteroids in polytene chromosomes ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 103-111 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Polytene Chromosomes ; Ecdysteroids ; Fat Body
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Changes in polytene chromosome 3 L puffing patterns in the fat body ofDrosophila melanogaster larvae and prepupae are compared to those in the salivary gland. While some general features are common to the two tissues, there are differences which reflect their different developmental roles. In vitro experiments with fat body chromosomes show that they have a distinct response to ecdysteroids which is different from that of salivary gland chromosomes, and which does not,in this culture system, reproduce the changes observed in normal development. In short term culture experiments, the fat body chromosomes appear more sensitive to ecdysteroids than the salivary gland chromosomes and, although 20-OH ecdysone is more active than ecdysone in these assays, the possibility is not excluded that ecdysone has a role in normal development as it appears to alter gene activity at physiological levels in these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848447
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  • 6
    Electronic Resource
    Electronic Resource
    Homologies of positional information in thoracic imaginal discs ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 293-300 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Imaginal discs ; Positional information ; Homology ; Intercalary regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The regulative behavior of fragments of the imaginal discs of the wing and first leg was studied when these fragments were combined with fragments of other thoracic imaginal discs. A fragment of the wing disc which does not normally regenerate when cultured could be stimulated to regenerate by combination with certain fragments of the haltere disc. When combined with a haltere disc fragment thought to be homologous by the criteria of morphology and the pattern of homoeotic transformation, such stimulated intercalary regeneration was not observed. Combinations of first and second leg disc fragments showed that a lateral first leg fragment could be stimulated to regenerate medial structures when combined with a medial second leg disc fragment but not when combined with a lateral second leg disc fragment. Combinations of wing and second leg disc fragments showed that one fragment of the second leg disc is capable of stimulating regeneration from a wing disc fragment while another second leg disc fragment fails to stimulate such regeneration. It is suggested that absence of intercalary regeneration in combinations of fragments of different thoracic imaginal discs is a result of homology or identity of the positional information residing in the cells of the fragments. The pattern of correspondence of positional information revealed by this analysis is consistant with the pattern of homology determined by morphological observation and by analysis of the positional specificity of homoeotic transformation among serially homologous appendages. The implications of the existence of homologous positional information in wing and second leg discs which share a common cell lineage early in development are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848488
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  • 7
    Electronic Resource
    Electronic Resource
    Gap junctions are non-randomly distributed inDrosophila wing discs (1982)
    Springer
    Development genes and evolution 191 (1982), S. 335-339 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Gap junction ; Wing disc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of gap junctions in mature larvalDrosophila melanogaster wing discs was analyzed by means of quantitative electron microscopy. Gap junctions are non-randomly distributed in the proximal-distal disc axis and in the apical-basal cell axis of the epithelium. In the epithelial cells, the surface density, number and length of gap junctions are greatest in the apical cell region and distal disc region. The average gap junction surface density is 0.0572 μm−1 and 2.77% of the lateral cell surface is composed of gap junctions. In the adepithelial cells, the gap junction surface density is 0.0005 μm−1 and 0.06% of the cell surface is composed of gap junctions. No gap junctions were observed between epithelial cells and adepithelial cells. The absolute area of gap junctions was estimated in a proximal-distal strip of cells in the disc and is considerably less in the folded regions of the epithelium compared to the flat notum and wing pouch regions. The results are discussed with respect to pattern formation and growth control in imaginal discs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848494
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  • 8
    Electronic Resource
    Electronic Resource
    Dicephalic — ADrosophila mutant affecting polarity in follicle organization and embryonic patterning (1982)
    Springer
    Development genes and evolution 191 (1982), S. 28-36 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Polarity ; Maternal effect ; Nurse cells ; Embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutationdicephalic (dic) affects follicle development and thereby alters the antero-posterior polarity of embryonic patterning. It maps at a single locus (3–46.0±1.0) and can be characterized as a semi-dominant maternal effect mutation with low penetrance. Indic follicles, the 15 nurse cells form two clusters located at opposite poles of the oocyte; the numerical distribution of the nurse cells among the clusters varies from 7:8 to 1:14. Thedic egg shell carries a micropyle (anterior marker) at either pole, but the misshapen respiratory appendages are restricted to one of the two poles in most eggs. The malformed eggs rarely yield larvae and these are always abnormal anteriorly and/or posteriorly. The segment pattern expressed in their cuticle may represent two anterior parts of opposite polarities (double head type), two posterior parts of opposite polarities (double abdomen type, rare) or show uniform polarity. Lability of organization at the cystocyte stage appears as the primary developmental defect of the mutant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848543
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  • 9
    Electronic Resource
    Electronic Resource
    A clonal analysis of cell lineage and growth in the male and female genital disc ofDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 42-55 
    Details
    ISSN: 1432-041X
    Keywords: Clonal analysis ; Growth ; Cell lineage ; Genital disc ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary InDrosophila, the terminalia (i.e. internal and external analia and genitalia, except the gonads) are formed by the genital disc. Comparative studies suggested that this disc may have evolved through fusion of the imaginal primordia of the last 3 or 4 abdominal segments. The present report describes the clonal relationships within the complex genital disc. Genetically marked cell clones were induced in male and female embryos and larvae heterozygous for cell marker mutations. 1) Frequencies and sizes of clones suggest that the embryonic disc anlage consists of 14–17 precursor cells: 4–6 for the analia, some 7 for the male genitalia, and 3–4 for the female genitalia. These cells grow exponentially during larval development. 2) In both sexes, the clones were confined to either analia or genitalia, suggesting two separate cell lineages already established at blastoderm. 3) Internal and external genitalia remain in the same compartment at least up to 60 h (end of first instar). 4) A clonal restriction appeared around 84 h (mid second instar), separating a dorsal from a ventral part in the male genitalia. The ventral compartment comprises the ventral part of the lateral plate and clasper, hypandrium, and all internal genitalia. No such boundary was detected in the female. 5) In the female, analia and parovaria originate from the same precursors; another cell lineage forms eighth tergites, vaginal plates, oviduct, receptacle, and spermathecae. 6) In female analia, dorsal and ventral plate share common precursors at least up to 84 h. A medio-lateral boundary may appear at 84 h in the ventral anal plate. No clonal restriction was found in the male analia. 7) At all times, clones could cross between left and right sides of the symmetrical terminalia; they consistently did so via ventral structures. 8) The results are discussed in a phylogenetic context, and we propose that the clonal relations reflect the evolution of the complex genital disc.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848545
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  • 10
    Electronic Resource
    Electronic Resource
    Maternal effects of zygotic mutants affecting early neurogenesis inDrosophila (1982)
    Springer
    Development genes and evolution 191 (1982), S. 191-201 
    Details
    ISSN: 1432-041X
    Keywords: Neurogenic mutants ; Maternal effects ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The size of the neurogenic region ofDrosophila melanogaster is under the control of several genes of zygotic expression. Lack of function from any of those genes produces an increase of the size of the neurogenic region at the expense of the epidermal anlage. However, differences exist in the extent of neuralisation achieved by each of the genetic loci upon mutation. The present results show that in the case ofN andmam phenotype differences are due to different contributions of maternal gene expression. This could be shown by studying the phenotype which appeared in mutant embryos when the oocytes developed from homozygous mutant precursor cells. Clones of mutant cells were induced in the germ line of females heterozygous for the neurogenic mutationin trans over germ line dependent, dominant female sterile mutations. After removing maternal information the phenotype ofN andmam mutants became identical in both cases. Furthermore maternal information fromN + was found to be necessary for viability of the wildtype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848335
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  • 11
    Electronic Resource
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    Clonal analysis ofsex-lethal, a gene needed for female sexual development inDrosophila melanogaster (1982)
    Springer
    Development genes and evolution 191 (1982), S. 211-214 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Clonal analysis ; Sex determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mutationSxl f , located on the X-chromosome, is a sex-limited recessive lethal that specifically kills 2X; 2A flies while it does not affect X; 2A flies (Cline 1978). We have analyzed the role ofSxl f on sex determination by a clonal analysis of a new spontaneous allele,Sxl fLS . Female embryos and larvae heterozygous forSxl fLS were irradiated at different times of development to generate homozygousSxl fLS clones which were recognized by linked marker mutations. We have studied the phenotype of such clones on sexually dimorphic regions of the fly (foreleg basitarsus, 5th, 6th and 7th tergites, analia and external genitalia). Despite their female (2X; 2A) chromosomal constitution, clones homozygous forSxl fLS differentiated male structures. These results confirm and extend the preliminary report of Cline (1979). They show that the wildtype product ofSxl f is required for female development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848339
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  • 12
    Electronic Resource
    Electronic Resource
    Tissue-specific and substrate-specific detection of aldehyde and pyridoxal oxidase in larval and imaginal tissues of Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 315-332 
    Details
    ISSN: 1573-4927
    Keywords: aldehyde oxidase ; pyridoxal oxidase ; tissue specificity ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The substrate specificities of aldehyde and pyridoxal oxidases in Drosophila melanogaster have been determined with a variety of aliphatic and aromatic aldehydes. This analysis has led to the discovery that 2,4,5-trimethoxy-benzaldehyde is a specific substrate for pyridoxal oxidase, as based on the histochemical distribution of oxidase activity, the absence of enzymatic activity in the lpo 1strains, and the dosage dependence on the number of lpo +genes present. The tissue-specific localization of aldehyde oxidase (AO) and pyridoxal oxidase (PO) in the larval and adult structures showed that AO was present in all the major internal organs of the larvae and adults, including brain, imaginal discs, Malpighian tubules, digestive system, and reproductive structures. Pyridoxal oxidase is present in many of the same structures which possess AO, but is missing from the cardia, crop, imaginal discs, ovarian follicle cells, paragonia, pericardial cells, and wreath cells. The only structure which possesses PO but lacks AO is the larval salivary gland. These histochemical differences in AO and PO distribution were also confirmed by enzymatic analysis of the activities present in homogenates of ovaries, paragonia, and salivary glands. The general pattern of enzyme expression appears to be established during embryogenesis and maintained throughout the life of the individual.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484427
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  • 13
    Electronic Resource
    Electronic Resource
    Selection at the α-Gpdh locus in experimental populations of Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 461-474 
    Details
    ISSN: 1573-4927
    Keywords: Drosophila ; allozymes ; α-Gpdh ; selection ; genetic background
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three sets of experiments have been conducted in order to evaluate the role of natural selection at the α-Gpdh locus in Drosophila melanogaster. (1) The evolution of the F-allele frequency has been followed for many generations in 13 experimental populations having different genetic backgrounds. (2) Egg-to-adult viability has been measured in synthetic populations derived from one locality (Brouilly) and the results have been compared with those of a previous experiment involving a different local population (Tostes). (3) The effects of sodium octanoate on egg-to-adult viability have been measured on the genotypes FF, FS, SF, and SS. The results demonstrate that selection operates on a small block of genes which includes the α-Gpdh locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484697
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  • 14
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    An esterase duplication in Drosophila: Differences in expression of duplicate loci within and among related species (1982)
    Springer
    Biochemical genetics 20 (1982), S. 929-942 
    Details
    ISSN: 1573-4927
    Keywords: esterase ; duplication ; gene expression ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An esterase duplication is described in the sibling species pair Drosophila mojavensis and Drosophila arizonensis. We present evidence for two separate structural loci mapping at a distance of less than 0.16 recombination units from each other. Alleles at the two loci have the same substrate specificities and form small amounts of interlocus heterodimers. One locus (Est-5) is functioning throughout the insect's life cycle and appears at high concentrations in the hemolymph and the fat body. Its duplicate (Est-4) functions only during the late larval stage and is concentrated mainly in the carcass. No null alleles at either locus were observed in population surveys. An examination of 12 other species from the repleta group, to which D. mojavensis and D. arizonesis belong, suggests that Est-5 is universally present, but the activity levels of Est-4 vary among species and may be totally absent in some species. Variation in the level of Est-4 activity does not closely follow the phylogenetic relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484070
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  • 15
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    The association between malathion resistance and acetylcholinesterase in Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 179-198 
    Details
    ISSN: 1573-4927
    Keywords: acetylcholinesterase ; Drosophila ; malathion ; insecticide resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relationship between the 50% survival time for flies feeding on a malathion-containing medium and the activity of acetylcholinesterase (AChE) was determined for 15 isofemale lines of Drosophila melanogaster. A significant correlation was found (r=0.28, P〈0.05), with more resistant lines tending to have a lower level of AChE activity. An association between AChE and malathion resistance was also observed in a selection experiment. The AChE activity decreased in two of two populations selected for malathion resistance. AChE from these populations was altered in kinetic parameters (measured in crude head extracts) and electrophoretic mobility. Although the “resistant” AChE had a lower activity (V m) on either a per milligram protein or a per individual basis, its apparent K m for acetylthiocholine was lower than that of “susceptible” AChE. Recombination mapping of both low activity and fast electrophoretic mobility localized these traits to the region of the structural locus (Ace) on the third chromosome. The AChE activity of flies heterozygous for a variety of Ace lesions (kindly provided by Dr. W. M. Gelbart) was consistent with this location. The changes in AChE were suggested to have been caused by selection of alleles at the Ace locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00484945
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  • 16
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    Genetic, ontogenetic, and tissue-specific variation of dipeptidases in Drosophila melanogaster (1982)
    Springer
    Biochemical genetics 20 (1982), S. 407-424 
    Details
    ISSN: 1573-4927
    Keywords: dipeptidases ; Drosophila ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three dipeptidases in Drosophila melanogaster are under independent genetic control and their structural genes have been localized, Dip-A to 2R and Dip-B and Dip-C to 3R (Voelker and Langley, 1978; Ohnishi and Voelker, 1981). These enzymes were characterized with respect to their substrate specificities, genetic variability (electrophoretic mobility and quantitative activity level), ontogeny (activity and isozyme pattern), and tissue localization. The dipeptide substrate specificities of DIP-A and DIP-B overlap each other considerably, but do not overlap with DIP-C. In natural populations, DIP-B and DIP-C are essentially monomorphic electrophoretically whereas DIP-A is polymorphic for three allozymes. Both DIP-A and DIP-B show quantitative genetic variation of activity level within an allozyme class. All three enzymes are expressed at all stages in the life cycle, but DIP-A and DIP-B activities vary considerably according to developmental stage and sex of adult. The tissue localizations of DIP-A and DIP-B activities show similar patterns and a nearly ubiquitous occurrence of both enzymes, but with particularly high values in larval and adult midguts and in the adult female reproductive system. These results suggest a general metabolic role for the enzymes, such as regulation of the concentrated pools of amino acids and oligopeptides found in Drosophila tissues.
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    URL: http://dx.doi.org/10.1007/BF00484692
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  • 17
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    Selection at the alcohol dehydrogenase locus of Drosophila melanogaster: Effects of ethanol and temperature (1982)
    Springer
    Biochemical genetics 20 (1982), S. 681-688 
    Details
    ISSN: 1573-4927
    Keywords: alcohol dehydrogenase ; Drosophila ; selection ; ethanol ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Drosophila melanogaster larvae were subjected to 10 generations of selection on 6% ethanol at 17, 25, and 30°C. For each temperature there was a significant (P〈0.01) increase in the frequency of the Adh isoallele. Controls with no ethanol showed no change in the frequency of the Adh F isoallele. Larvae subjected to stronger selection on 8% ethanol confirmed the results. When adults of various ages were subjected to 16 and 32°C, the ADHF isoenzyme retained its twofold advantage in activity over ADHS regardless of the temperature. The same result was obtained with larvae at 16 and 35°C. Although some effect of temperature was demonstrated, it was concluded that the effect was not strong enough for temperature to be a selective factor under the conditions studied. However, ethanol is a strong selective factor for laboratory populations.
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    URL: http://dx.doi.org/10.1007/BF00483965
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  • 18
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    Underreplication during polytenization? (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 193-199 
    Details
    ISSN: 1432-2242
    Keywords: Drosophila ; Polytene nuclei ; Underreplication ; Polytenization ; Cytophotometry ; Heterochromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recent cytophotometric DNA determinations and results of labeling experiments are compared with results of biochemical experiments concerning larval polytene salivary gland nuclei of Drosophila melanogaster. Recent publications (Dennhöfer 1981; 1982 a, b) demonstrate that methodological errors both in hydrolysis of the DNA before Feulgen reaction and in interpretation of the cytophotometric values give raise to the hypothesis of heterochromatic underreplication during polytenization. It is concluded also that methodological difficulties cause the absence of polytene SAT-DNA in biochemical centrifugation experiments since, because of different solubilities of eu- and heterochromatic DNA, the latter is not resolved in DNA isolation procedures from polytene nuclei.
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    URL: http://dx.doi.org/10.1007/BF00303991
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  • 19
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    Genetic variation for anemotaxis (wind-directed movement) in laboratory and wild-caught populations ofDrosophilia (1982)
    Springer
    Behavior genetics 12 (1982), S. 281-293 
    Details
    ISSN: 1573-3297
    Keywords: anemotaxis ; Drosophila ; habitat selection ; heritability ; wind-directed movement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Two strains ofDrosophila melanogaster were selected for anemotactic response for six generations—one line for upwind response and one line for downwind response. A realized heritability estimate ofh 2=0.131 ±0.029 was obtained for the upwind response, and a realized heritability estimate ofh 2=0.012±0.014 was obtained for the downwind response. The divergent selection estimate wash 2=0.031±0.013. These values are consistent with previously reported heritability estimates for phototaxis and geotaxis, and serve to suggest that wind-oriented movement can be rapidly modified by selection under different habitat conditions. A comparison of wind response among wild-caught individuals of 11 species shows significant response differences between closely related species. Evaluation of these differences in light of the ecology of the flies suggests that upwind movement occurs among the monophagous species, which must move long distances to find their specific feeding sites, while downwind movement is more typical of polyphagous species. Species which are found in riparian or montane forest conditions showed a general reluctance to move under windy conditions. This corresponds to previous observations on these species and reflects the absence of wind generally encountered by these species during their natural periods of activity.
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    URL: http://dx.doi.org/10.1007/BF01067848
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  • 20
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    Effets de la mise en eau du réservoir Desaulniers (Territoire de la Baie de James) sur le zooplancton d'une rivière et d'une tourbière reticulée (1982)
    Springer
    Hydrobiologia 86 (1982), S. 271-296 
    Details
    ISSN: 1573-5117
    Keywords: zooplancton ; phénologie ; mise en eau ; reservoir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper presents the results of an investigation which lasted three years, on the effects of impounding a river on its zooplankton community. Forty zooplankton samples were collected from the Desaulniers River and from a string bog (53°35′N, 77°35′W) in 1976 before impoundment. Sampling was also conducted in 1977 (331 samples) and 1978 (153 samples) during and after impoundment. The population dynamics of zooplankton show different patterns in 1977 and 1978. The main impacts of impoundment on the river and the bog communities are the increase of the population density, particulary of Rotifera and Copepoda, the increase of the number of species of Rotifera and the increase of the diversity indices based on numbers and biomass. These changes are observed at each sampling station. The total biomass increases in lotic stations but decreases in the string bog because the density and the biomass of Cladocera increases in the river but drops in the bog. Results are compared to those obtained in natural lakes and bog of the same area and to those published in other studies.
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    Rutland 2000: educational resources of Rutland water (1982)
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    Hydrobiologia 88 (1982), S. 55-55 
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    ISSN: 1573-5117
    Keywords: reservoir ; education
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00008296
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    Rutland water phytoplankton: the development of an asset or a nuisance? (1982)
    Springer
    Hydrobiologia 88 (1982), S. 117-133 
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    ISSN: 1573-5117
    Keywords: phytoplankton ; reservoir ; succession ; abundance ; control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of a seasonal succession of phytoplankton species in Rutland Water is described. The factors controlling their succession and abundance are discussed in relation to changes in physical and chemical variables. At present, input of water from the catchment and by pumping from the Rivers Welland and Nene, has the greatest influence on phytoplankton abundance.
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    Bathymetric distribution of some Chironomidae (Diptera) in Rutland Water (1982)
    Springer
    Hydrobiologia 88 (1982), S. 159-169 
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    ISSN: 1573-5117
    Keywords: Chironomidae ; bathymetric ; distribution ; Rutland Water ; reservoir ; SPSS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bathymetric distribution of the eight most abundant taxa of chironomid larvae is described from benthic grab samples collected in Rutland Water from May 1977 to April 1979. Cricotopus, Psectrocladius and Endochironomus larvae were generally restricted to water less than 9 m deep. Procladius, Chironomus, Microtendipes, Polypedilum and Tanytarsini larvae were all recorded at depths down to 25 m. However, the highest population densities of the latter five taxa were recorded in shallow water. The results are discussed in relation to the diet of trout and certain management practices at the reservoir. Also described is the application of SPSS (Statistical Package for the Social Sciences) to the analysis of these data.
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    Rutland Water Nature Reserve: concept, design and management (1982)
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    Hydrobiologia 88 (1982), S. 211-224 
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    ISSN: 1573-5117
    Keywords: nature reserve ; reservoir ; planning ; design ; management ; wildfowl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper illustrates how practical conservation management has helped to create a nature reserve of international importance for wildfowl. The development of existing and introduced habitats has produced a wide diversity of fauna and flora without causing an unnatural ecological balance and close liaison with other reservoir users has establoshed zoning areas free from disturbance resulting in both man and birds benefiting from such agreements.
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    Distribution of chironomids in the littoral zone of Lake Texoma, Oklahoma and Texas (1982)
    Springer
    Hydrobiologia 89 (1982), S. 177-188 
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    ISSN: 1573-5117
    Keywords: Chironomidae ; littoral ; reservoir ; salinity ; chlorides ; particulate organic matter ; temperature ; wind
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lake Texoma in southcentral Oklahoma was formed by the impoundment of the Washita and Red Rivers. The Red River is more highly saline than the Washita and creates a complex salinity gradient across the reservoir. Populations of chironomids were monitored with multiple-plate samplers in areas of high (34–113 mg l−1 Cl−), intermediate (35–60 mg l−1 Cl−) and low (4–27 mg l−1 Cl−) salinity during the spring and summer of 1978. Food availability, temperature, salinity and wind direction influenced the distribution of the 14 genera and at least 22 species of chironomids which colonized the multiple-plate samplers. Filter-feeders attained their highest densities in the river-arm stations where levels of particulate organic matter (POM) were high. Algal grazers attained their highest densities in the clear intermediate area where the plates of the samplers were covered with algal mats. Most of the genera believed to be feeding primarily on POM decreased in density as the temperature and density of Glyptotendipes sp. rose. Certain species were restricted to either the Red River arm or the Washita River arm and this is probably a reflection of different salinity tolerances.
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    Factors affecting depth distribution of dragonflies and other benthic insects in a thermally destabilized reservoir (1982)
    Springer
    Hydrobiologia 87 (1982), S. 33-44 
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    ISSN: 1573-5117
    Keywords: benthos ; depth distribution ; Diptera ; dragonfly ; Ephemeroptera ; Odonata ; reservoir ; South Carolina ; thermal effluent ; Trichoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution and abundance of macroinvertebrates along a water depth gradient were studied in thermal and ambient temperature areas of a reservoir in the southeastern United States. Benthic samples were taken at 10 depths (0.16–5 m) during January–April, 1978. Factors affecting the depth distribution of Ceratopogonidae, Chaoboridae, Chironomidae, Ephemeroptera, Odonata, and Trichoptera are discussed. The relationship between abundance of organisms and water depth was more complex than an inverse relationship generally reported in the literature.
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    The landscape of Rutland Water (1982)
    Springer
    Hydrobiologia 88 (1982), S. 43-46 
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    ISSN: 1573-5117
    Keywords: Rutland Water ; landscape ; amenity planning ; tree planting ; reservoir ; draw-down
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The landscape problems encountered at Rutland Water were: 1) the reconciliation of the reservoir and its works with the rural surroundings; 2) siting and treatment of all ancillary works; 3) mitigation of exposure of shore by draw-down; 4) the provision of opportunities for recreation without damage to the environment; and 5) conservation of nature. The means adopted were: 1) forward planning; 2) consultation and co-operation with all interests concerned; 3) extensive planting of native species and of ground shaping; and 4) arrangements for appropriate maintenance.
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    Rutland water raw water quality — problems and management (1982)
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    Hydrobiologia 88 (1982), S. 93-101 
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    ISSN: 1573-5117
    Keywords: reservoir ; raw water ; management ; chemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The options available for the management of Rutland Water are identified and their use discussed. The changes in the chemistry of the stored water with regard to sulphate, silica (molybdate-reactive), dissolved reactive phosphate and total oxidised nitrogen are shown.
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    Effects of sedimentation on the storage capacity of the High Aswan Dam reservoir (1982)
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    Hydrobiologia 91-92 (1982), S. 623-639 
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    ISSN: 1573-5117
    Keywords: reservoir ; sedimentation ; River Nile ; High Aswan Dam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The River Nile receives most of its sediment load from the Atbara and Blue Nile rivers, which carry eroded sediments north from the Ethiopian mountains during the seasonal flood period between August and October. Prior to the construction and operation of the High Aswan Dam, in 1964, 9–10 × 106 metric tons of suspended sediment were deposited annually in the flood plain of the Nile, while about 93% of the total average annual suspended load of 124 × 106 metric tons was carried out into the Mediterranean Sea. Since the full operation of the High Aswan Dam in 1968, the flood discharge of the Nile, below the dam, has been greatly modified and more than 98% of the total suspended load has been retained within the reservoir. Based on long-term records; estimated relationships between discharge and suspended load, and field measurements, the life span of the dead storage capacity has been estimated at a minimum of about 360 years. Although this preliminary calculation is less than the estimated design capacity of 450 years, it is expected that progressively more suspended solids will be released in the outflow of the reservoir and that together with the use of flood diversion schemes the High Aswan Dam is likely to approach its design life span.
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    Effects of sedimentation on the storage capacity of the High Aswan Dam reservoir (1982)
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    Hydrobiologia 91-92 (1982), S. 623-639 
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    ISSN: 1573-5117
    Keywords: reservoir ; sedimentation ; River Nile ; High Aswan Dam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The River Nile receives most of its sediment load from the Atbara and Blue Nile rivers, which carry eroded sediments north from the Ethiopian mountains during the seasonal flood period between August and October. Prior to the construction and operation of the High Aswan Dam, in 1964, 9–10 × 106 metric tons of suspended sediment were deposited annually in the flood plain of the Nile, while about 93% of the total average annual suspended load of 124 × 106 metric tons was carried out into the Mediterranean Sea. Since the full operation of the High Aswan Dam in 1968, the flood discharge of the Nile, below the dam, has been greatly modified and more than 98% of the total suspended load has been retained within the reservoir. Based on long-term records; estimated relationships between discharge and suspended load, and field measurements, the life span of the dead storage capacity has been estimated at a minimum of about 360 years. Although this preliminary calculation is less than the estimated design capacity of 450 years, it is expected that progressively more suspended solids will be released in the outflow of the reservoir and that together with the use of flood diversion schemes the High Aswan Dam is likely to approach its design life span.
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    Effects of sediment releases from a reservoir on stream macroinvertebrates (1982)
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    Hydrobiologia 96 (1982), S. 177-184 
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    ISSN: 1573-5117
    Keywords: sediment ; reservoir ; stream ; macroinvertebrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effects of sediment release from Guernsey Reservoir on macroinvertebrates of the North Platte River, Wyoming, were investigated during summer 1981. Suspended solids concentrations during sediment release increased from 〈20 mg l−1 to 〉300 mg l−1. Because fine particulates remained in suspension, mean particle size of substrates was unaltered. Densities of chironomids decreased 90% + during sediment release but recovered to initial levels in 3 weeks after the release ended. Densities of mayflies and oligochaetes increased. Changes in benthic populations were highly correlated with increases in suspended solids.
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    Area structure plans of Leicestershire County and Rutland District Councils (1982)
    Springer
    Hydrobiologia 88 (1982), S. 52-52 
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    ISSN: 1573-5117
    Keywords: reservoir ; planning policies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The poster summarised Leicestershire County and Rutland District Councils Planning policies operative in the area surrounding Rutland Water. The policies are contained in the Structure Plan for Rutland, approved in December 1979, Rutland District Council's Settlement Policy and the Empingham District Plan preliminary discussion document. The policies of more specific application to the area surrounding Rutland Water are those of the Empingham District Plan which relate to land use, recreation and traffic management in the Parishes of Burley, Edith Weston, Egleton, Empingham, Exton, Gunthorpe, Hambleton, Horn, Lyndon, Manton, Normanton and Whitwell. The theme reflected in all policies covering this area is one of conservation. It is expressed in one of the Structure Plan's basic policies as follows: ‘Rutland's Planning Role should be one of positive conservation particularly of natural resources with an emphasis on minimising the loss of, and where practicable improving the natural assets of farmland, woodland, minerals and water.’
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    The effect of Langmuir circulation on the distribution and settling of algae and suspended particles (1982)
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    Hydrobiologia 88 (1982), S. 88-88 
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    ISSN: 1573-5117
    Keywords: reservoir ; Langmuir circulation ; algae ; sedimentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The quantitative extent to which the large-scale organised water motion in the surface waters of lakes and reservoirs, known as Langmuir circulation, affects the distribution and settling of algae and other suspended particles is not known and has thus been ignored in conventionally used water quality models. Since the distribution and settling of these particles is important in determining water quality, this study set out to investigate these effects using a mathematical model based on the two-dimensional advection-diffusion mass transport equation describing the temporal and spatial distribution of suspended particles in a typical Langmuir cell. The Langmuir circulation flow field and turbulent diffusion coefficients are empirically modelled by relating them to environmental parameters (Buranathanitt et al., in press). The results show that Langmuir circulation does affect particle distribution and settling. For particles with small sinking speeds, such as the lighter algae, the circulation causes intense mixing, resulting in essentially uniform distribution of particles over the cell (as assumed in the ‘well-mixed compartment model’). For particles with high sinking velocities, however, aggregation can occur, giving rise to significant reduction in sinking loss. For diatoms, effective sinking speeds are as much as 6% less than when cells are not operating while for coarse silt particles in a cell of large width to depth ratio a reduction of more than 60% is possible. Two preliminary laboratory experiments have also been performed in order to obtain further information on both the physical characteristics of Langmuir cells and their effects on suspended particles. The results of a wind-wave tank experiment suggest that the Langmuir circulation scale of motion is related to the height of the surface waves, thus providing an empirical means of determining the size of Langmuir cells from environmental variables. A particle settling tank experiment confirmed, in a qualitative way, the results obtained in the analytical study, with both the uniformly mixed and aggregation phenomena being reproduced (Buranathanitti et al., submitted). Full details of the work described in this abstract will be published elsewhere (Buranathanitt et al., in press; Buranathanitt et al., submitted).)
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    Establishing and maintaining the trout fishery at Rutland Water (1982)
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    Hydrobiologia 88 (1982), S. 179-189 
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    ISSN: 1573-5117
    Keywords: reservoir ; trout ; angling ; impoundment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rutland Water was stocked with trout in 1975 after removing the existing fish populations. Growth and survival of rainbow and brown trout are compared with the reservoir's filling regime, and re-colonisation by other fish species is described. The use of angler success as an indicator of fish stock density and its management is discussed.
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    Benthic invertebrates of Rutland Water (1982)
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    Hydrobiologia 88 (1982), S. 147-156 
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    ISSN: 1573-5117
    Keywords: reservoir ; benthos ; invertebrates ; colonisation ; abundance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The colonisation of Rutland Water by benthic invertebrates has been monitored from 1976 when the reservoir was first filled. A modified Ekman grab was used to sample populations quantitatively while false substrates and dredge samples were used to obtain supplementary data. Hand net and hand-sorting were used to study the littoral zone. The dominant taxa in the reservoir are Asellus aquaticus, A. meridianus, Gammarus pulex, Crangonyx pseudogracilis, Helobdella stagnalis, Erpobdella octoculata, Polycelis tenuis, larval Chironomidae and Oligochaeta. Other species which appeared abundantly, such as Limnaea pereger and Planorbis albus, have now disappeared and it is suggested that the generally low levels of gastropods might account for the poor performance of species such as Dugesia lugubris and Glossiphonia complanata. Other aspects of abundance are discussed, as are the effects of maturation of the system.
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    Temporal variations in the diet of brown trout (Salmo trutta L.) and rainbow trout (S. gairdneri R.) in Rutland Water (1982)
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    Hydrobiologia 88 (1982), S. 199-206 
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    ISSN: 1573-5117
    Keywords: trout ; Salmo trutta ; Salmo gairdneri ; diet ; Rutland Water ; reservoir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The diet of brown trout (Salmo trutta L.) and rainbow trout (S. gairdneri Richardson) in Rutland Water were compared during the first two fishing seasons (April–October 1977 and 1978). Fortnightly samples of approximately forty stomachs were obtained from boat and bank, rod-and-line caught trout giving a total of 1046 stomachs over the two seasons. During 1977 seasonal changes in the diet were divided into two phases; the first being a period of abundant drowned terrestrial food until June. This was followed by a period of more stable water level from July onwards when chironomid larvae and pupae were consistently the most important food items and the diversity of food also increased. In 1978 the proportion of chironomid pupae and larvae declined and they were replaced in the diet by Gammarus and Asellus.
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    Rutland Water — from conception to operation (1982)
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    Hydrobiologia 88 (1982), S. 7-17 
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    ISSN: 1573-5117
    Keywords: Rutland Water ; reservoir ; water supply ; demand ; design ; recreation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rutland Water was planned in the 1960s to meet the water needs of the expanding East Midland region of England. Chosen from 64 possible sites, it was approved in 1970 and construction started in 1971. The dam was closed and the reservoir started to fill from its natural catchment in 1975; pumping started from the rivers Welland and Nene in 1976 and the reservoir was full by March 1979. Details of the stages in its development are given together with an indication of the ways in which the recreational uses of the reservoir have been integrated with its water supply function.
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    The feeding of trout during the filling phase of Rutland Water (1982)
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    Hydrobiologia 88 (1982), S. 191-198 
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    ISSN: 1573-5117
    Keywords: trout ; littoral fauna ; impoundment ; flooding ; reservoir ; Rutland Water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fingerling Salmo trutta L. and S. gairdneri R. were stocked in Rutland Water in early 1975. Initial rapid growth was associated with feeding on littoral and planktonic Crustacea during this year. Rapid water level changes occurred over the winter months, interspersed with longer periods of stability during the summer. These changes caused changes in trout diet. Terrestrial food (e.g. earthworms), released from flooded land was important during winter months; aquatic items which increased in abundance during periods of stable water levels (e.g. Gammarus pulex, Limnaea pereger, Chironomidae larvae and pupae) were important during spring, summer and autumn. It is suggested that the rapid growth rates recorded in the first two years of the reservoir are the result of the ready availability of different food sources.
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    Effects of sedimentation on the storage capacity of the High Aswan Dam reservoir (1982)
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    Hydrobiologia 91-92 (1982), S. 623-639 
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    ISSN: 1573-5117
    Keywords: reservoir ; sedimentation ; River Nile ; High Aswan Dam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The River Nile receives most of its sediment load from the Atbara and Blue Nile rivers, which carry eroded sediments north from the Ethiopian mountains during the seasonal flood period between August and October. Prior to the construction and operation of the High Aswan Dam, in 1964, 9–10 × 106 metric tons of suspended sediment were deposited annually in the flood plain of the Nile, while about 93% of the total average annual suspended load of 124 × 106 metric tons was carried out into the Mediterranean Sea. Since the full operation of the High Aswan Dam in 1968, the flood discharge of the Nile, below the dam, has been greatly modified and more than 98% of the total suspended load has been retained within the reservoir. Based on long-term records; estimated relationships between discharge and suspended load, and field measurements, the life span of the dead storage capacity has been estimated at a minimum of about 360 years. Although this preliminary calculation is less than the estimated design capacity of 450 years, it is expected that progressively more suspended solids will be released in the outflow of the reservoir and that together with the use of flood diversion schemes the High Aswan Dam is likely to approach its design life span.
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    Tree pre-planting at Rutland Water (1982)
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    Hydrobiologia 88 (1982), S. 51-51 
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    ISSN: 1573-5117
    Keywords: tree-planting ; reservoir ; drawdown
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aesthetic problems posed by reservoir margins have been recognised for many years. They stem from a complex pattern of action and interaction of several environmental variables — water manipulation patterns, wave action, substrate characteristics, grazing and other factors. These factors usually result in reservoir margins being not only visually unattractive and physically unstable, but also of low ecological diversity. The Natural Environment Research Council and Water Research Centre have, since 1968, together funded a research programme to investigate the use of plant species to combat these problems. In isolated instances in the UK, woody species in particular have succeeded in colonising margins naturally, to very good visual effect (e.g. at Lake Vyrnwy). ‘Biotechnical’ treatments — using artificially planted shrubs and trees — have been successfully used in large Central European reservoirs to combat soil erosion by waves. Following trials of various woody species on the margins of established reservoirs, 10 species/cultivars were ‘pre-planted’ above and below the future top water level (TWL) of Rutland Water in 1973, 5 years before reservoir filling was completed. Pre-planting theoretically allows better survival by trees of subsequent partial inundation. The precise siting of pre-planted trees in the vertical dimension should normally be done using modelled predictions of reservoir summer median water level (a variable important to tree survival). The complex factors affecting the operation of Rutland Water made precise prediction impossible; the experimental plot eventually extended from 500 mm above TWL, to 2 000 mm below TWL, in 500 mm steps. 252 trees were involved in the plot and their growth has been monitored for the past 7 years. The species being predominantly hydrophytic, drought was a problem in the years prior to reservoir filling. Mulching with grass-cuttings proved to be helpful in this respect. Subsequently, prolonged periods of high water level have been deleterious to tree survival in the lower areas of the plot. Once abstraction/recharging patterns become more cyclic, it is anticipated that tree growth will stabilise.
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    Traffic and visitor surveys at Rutland Water (1982)
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    Hydrobiologia 88 (1982), S. 53-54 
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    ISSN: 1573-5117
    Keywords: reservoir ; visitors ; traffic ; amenity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    The production of public supply drinking water from Rutland Water at the wing treatment works (1982)
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    Hydrobiologia 88 (1982), S. 103-116 
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    ISSN: 1573-5117
    Keywords: reservoir ; treatment ; pulsator ; quality ; haloforms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The paper describes the treatment facilities and processes at the Wing Works where Rutland water is treated to potable standards. It summarises the experience gained in the operation of the works since it opened in 1977 and includes a brief account of an investigation into the production of haloforms.
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    The effects of cytochalasin B and colchicine on the morphogenesis of mitochondria in Drosophila hydei during meiosis and early spermiogenesis (1982)
    Springer
    Cell & tissue research 224 (1982), S. 161-168 
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    ISSN: 1432-0878
    Keywords: Mitochondria ; Meiosis ; Spermiogenesis ; Drosophila ; Organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Morphogenesis of mitochondria in male germ cells in cultivated cytocysts begins in early prophase I at which time mitochondria thicken and become ordered along the spindle apparatus during meiosis. At the end of the second meiotic division they aggregate to form the Nebenkern. In the presence of colchicine or cytochalasin B mitochondria are able to begin differentiation, although the correct course of meiosis is not guaranteed. In medium supplemented with colchicine they undergo normal thickening but do not aggregate, in a pattern known from untreated cultures. This may indicate that microtubules are involved in the aggregation process of mitochondria as colchicine is known to inhibit microtubule formation. Moreover, in cell cultures treated with cytochalasin B mitochondrial aggregation does occur; it is concluded that microfilaments, which are sensitive to cytochalasin B, do not play a detectable role in the aggregation of mitochondria.
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    URL: http://dx.doi.org/10.1007/BF00217275
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  • 44
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    Ultrastructural pathology of the compound eye and optic neuropiles of the retinal degeneration mutant (w rdgB KS222) Drosophila melanogaster (1982)
    Springer
    Cell & tissue research 225 (1982), S. 11-22 
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    ISSN: 1432-0878
    Keywords: Transmission and high voltage electron microscopy ; Drosophila ; Degeneration ; Retinular cells ; Optic neuropiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The compound eye and the two most distal optic neuropils (lamina ganglionaris and medulla externa) of the Drosophila mutant w rdgB KS222were examined with transmission electron microscopes at conventional (60 kV) and high (0.8–1 MV) voltages. Eye tissue was sampled in the newly emerged and at 3, 7, and 21 days following eclosion. This mutant is known to show a light-induced degeneration of the peripheral retinular cells (R 1–6); the spectral sensitivity is altered and the threshold is increased reflecting the function of the central cells (R7, 8) which do not degenerate. A totally normal appearing visual system (peripheral retina and optic neuropiles) was found in newly emerged adults. After 3 days the somata of some of the peripheral retinal cells are affected and all of their axons show degeneration. At one week the R 1–6 pathology is well advanced in both somal and axonal regions. In affected cells the cytoplasm is more or less uniformly electron dense and contains liposomes, lysosome-like bodies, myeloid figures and vacuoles suggesting autophagy. Such cytoplasm (noted at 3 and 7 days post-eclosion) exhibits an electron dense reticulum and degenerate mitochondria. Microvilli become more electron dense. Retinular axon terminals are electron opaque and lack synaptic vesicles with few if any presynaptic structures. Mitochondrial remains are barely recognizable. Transsynaptic degeneration was not found. After 3 weeks, the structure of R 1–6 in the peripheral retina (somata and rhabdomeres) is greatly reduced or lost while R7 and R8 and higher order neurons are not affected. The debris from cell bodies and axon terminals of R 1–6 seems diminished, so that some phagocytosis probably takes place along with gliosis in the lamina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216214
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  • 45
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    Electronic Resource
    Comparative study of structure and function of blood cells from two Drosophila species (1982)
    Springer
    Cell & tissue research 221 (1982), S. 607-615 
    Details
    ISSN: 1432-0878
    Keywords: Hemocytes ; Drosophila ; Ultrastructure ; Phagocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Hemocytes of Drosophila melanogaster and Drosophila yakuba larvae have been defined in terms of their ultrastructure and functions in “coagulation”, wound healing, encapsulation, phenol-oxydase activity, and phagocytosis. The position of these cells among the classical hemocyte types of insects is determined. We distinguish two plasmatocyte types (macrophage plasmatocytes and lamellocytes) which do not seem to belong to the same lineage, and oenocytoids which are the crystal cells of the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215704
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  • 46
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    Electronic Resource
    Autoradiographic studies of protein and polysaccharide synthesis during vitellogenesis in Drosophila (1982)
    Springer
    Cell & tissue research 224 (1982), S. 315-333 
    Details
    ISSN: 1432-0878
    Keywords: Oogenesis ; Vitellogenesis ; Radiolabelling ; Metabolism ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Quantitative light- and electron-microscopic autoradiography was used to evaluate metabolic processes that occur during late developmental stages (10–14) of oogenesis in Drosophila melanogaster. Major differences in radiolabelling patterns were found after in vivo (10–45 min) uptake of [3H]-monosaccharides and [3H]-L-lysine. Several different methods of data analysis were required to facilitate interpretation of these patterns. [3H]-L-lysine produced extensive cytoplasmic labelling at all developmental stages. In addition, about 15% of alpha yolk spheres were intensely labelled at stage 10, reflecting the incorporation of radiolabelled vitellogenins synthesized during the incubation period. Subsequent stages showed low silver grain density over alpha yolk spheres until stage 14, when a burst of [3H]-L-lysine incorporation by most alpha spheres was observed, possibly indicative of a maturation process for embryogenesis. [3H]-D-glucose and [3H]-D-galactose (10 min, in vivo) both induced intense labelling of the beta yolk spheres in a manner suggesting in situ assembly beginning at early stage 13. Inasmuch as the polysaccharide of beta yolk spheres has the properties of glycogen (e.g., rosette structure digested by α-amylase) and the radiolabelled monosaccharides were introduced intraabdominally, it is evident that transport systems as well as enzymes utilizing glucose and galactose for glycogenesis must be readily available. It is notable that wide-spread labelling of egg chambers was elicited by [3H]-D-glucose and [3H]-D-galactose (e.g., nurse cells, follicle cells, chorion, vitelline membrane), but the labelling induced by [3H]-N-acetylmannosamine was restricted mainly to the endochorion. A possible role of microtubules in distribution and assembly of yolk spheres was inferred when colchicine, admixed to the culture medium (2–5 ppm), produced abnormal distribution and diminution in number of both alpha and beta yolk spheres. In addition to revealing previously unknown metabolic events of vitellogenesis, the results provide additional criteria for stage characterization as well as a means to specifically label certain macromolecules for purposes of isolation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216876
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