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Underreplication during polytenization?

Recent cytophotometric DNA determinations and related biochemical results concerning polytene salivary gland nuclei of Drosophila melanogaster

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Summary

Recent cytophotometric DNA determinations and results of labeling experiments are compared with results of biochemical experiments concerning larval polytene salivary gland nuclei of Drosophila melanogaster. Recent publications (Dennhöfer 1981; 1982 a, b) demonstrate that methodological errors both in hydrolysis of the DNA before Feulgen reaction and in interpretation of the cytophotometric values give raise to the hypothesis of heterochromatic underreplication during polytenization. It is concluded also that methodological difficulties cause the absence of polytene SAT-DNA in biochemical centrifugation experiments since, because of different solubilities of eu- and heterochromatic DNA, the latter is not resolved in DNA isolation procedures from polytene nuclei.

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Literature

  • Berger, C.A. (1938): Multiplication and reduction of somatic chromosome groups as a regular developmental process in the mosquito, Culex pipiens. Contributions to Embryology, Publ. Carnegie Inst. Nr. 167

  • Blumenfeld, M.; Forrest, H.S. (1971): Is Drosophila dAT on the Y-chromosome? Proc. Natl. Acad. Sci. USA 68, 3145–3149

    Google Scholar 

  • Blumenfeld, M.; Forrest, H.S. (1972): Differential under-replication of satellite DNAs during Drosophila development. Nat. New Biol. 239, 170–172

    Google Scholar 

  • Blumenfeld, M.; Orf, J.W.; Sina, B.J.; Kreber, R.A.; Callahan, M.A.; Mullins, J.I.; Snyder, L.A. (1978): Correlation between phosphorylated H1 histones and satellite DNAs in Drosophila virilis. Proc. Natl. Acad. Sci. USA 75, 866–870

    Google Scholar 

  • Botchan, M.; Kram, R.; Schmid, C.W.; Hearst J.E. (1971): Isolation and chromosomal localization of highly repeated DNA sequences in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 68, 1125–1129

    Google Scholar 

  • Brutlag, D.; Appels, R.; Dennis, E.S.; Peacock, W.J. (1977a): Highly repeated DNA in Drosophila melanogaster. J. Mol. Biol. 112, 31–47

    Google Scholar 

  • Brutlag, D.; Carlson, M.; Fry, K.; Hsieh, T.S. (1977b): DNA sequence organization in Drosophila heterochromatin. Cold Spring Harbor Symp. Quant. Biol. 42, 1137–1146

    Google Scholar 

  • Cohen, L.H.; Gotchel, B.V. (1971): Histones of polytene and nonpolytene nuclei of Drosophila melanogaster. J. Biol. Chem. 246, 1841–1848

    Google Scholar 

  • Cohen, E.H.; Bowman, S.C. (1979): Detection and location of three simple sequence DNAs in polytene chromosomes from virilis group species of Drosophila. Chromosoma 73, 327–355

    Google Scholar 

  • Cordeiro, M.; Wheeler, L.; Lee, C.S.; Kastritis, C.D.; Richardson, R.H. (1975): Heterochromatic chromosomes and satellite DNAs in Drosophila nasutoides. Chromosoma 51, 65–73

    Google Scholar 

  • Dennhöfer, L. (1981): Complete replication of DNA in polytene nuclei of salivary glands of Drosophila melanogaster. Wilh. Roux's Arch. Dev. Biol. 190, 237–240

    Google Scholar 

  • Dennhöfer, L. (1982a): Cytophotometric DNA determinations and replication pattern in salivary gland nuclei from larvae with different karyotypes in Drosophila melanogaster. Chromosoma (In press)

  • Dennhöfer, L. (1982b): Growth of polytene salivary gland nuclei from larvae with different karyotypes in Drosophila melanogaster. Wilh. Roux's Arch. Dev. Biol. (Submitted)

  • Dickson, E.; Boyd, J.B.; Laird Ch.D. (1971): Sequence diversity of polytene chromosome DNA from Drosophila hydei. J. Mol. Biol. 61, 615–627

    Google Scholar 

  • Elgin, S.C.R.; Weintraub, H. (1975): Chromosomal proteins and chromatin structure. Ann. Rev. Biochem. 44, 725–774

    Google Scholar 

  • Endow, S.A.; Gall, J.G. (1975): Differential replication of satellite DNA in polyploid tissues of Drosophila virilis. Chromosoma 50, 175–192

    Google Scholar 

  • Endow, S.A.; Polan, M.L.; Gall, J.G. (1975): Satellite DNA sequences of Drosophila melanogaster. J. Mol. Biol. 96, 665–692

    Google Scholar 

  • Endow, S.A.; Glover, D.M. (1979): Differential replication of ribosomal gene repeats in polytene nuclei of Drosophila. Cell 17, 597–605

    Google Scholar 

  • Entingh, T.D. (1970): DNA hybridization in the genus Drosophila. Genetics 66, 55–68

    Google Scholar 

  • Fox, D.P. (1970): A non-doubling DNA series in somatic tissues of the locusts Schistocerca gregaria (Foskal) and Locusta migratoria (Linn.). Chromosoma 29, 446–461

    Google Scholar 

  • Gage, L.P. (1974): Polyploidization of the silk gland of Bombyx mori. J. Mol. Biol. 86, 97–108

    Google Scholar 

  • Gall, J.G.; Cohen, E.H.; Polan, M.L. (1971): Repetitive DNA sequences in Drosophila. Chromosoma 33, 319–344

    Google Scholar 

  • Grimm, Ch.; Kunz W. (1980): Disproportionate rDNA replication does occur in diploid tissue of Drosophila hydei. Mol. Gen. Genet. 180, 23–26

    Google Scholar 

  • Gurley, L.R.; Walters, R.A.; Barham, S.S.; Deaven, L.L. (1978): Heterochromatin and histone phosphorylation. Expl. Cell Res. 111, 373–383

    Google Scholar 

  • Heitz, E. (1934): Über α- und β-Heterochromatin sowie Konstanz und Bau der Chromosomen bei Drosophila. Biol. Zentralbl. 54, 588–609

    Google Scholar 

  • Helmsing, P.J.; van Eupen, O. (1973): Chromatin of Drosophila: isolation and characterization of chromatin of two larval cell types. Biochim. Biophys. Acta 308, 154–160

    Google Scholar 

  • Hennig, W. (1972): Highly repetitive DNA sequence in the genome of Drosophila hydei. 2: Occurrence in polytene tissues. J. Mol. Biol. 71, 419–431

    Google Scholar 

  • Hennig, W.; Meer, B. (1971): Reduced polyteny of rRNA cistrons in giant chromosomes of Drosophila hydei. Nature (London), New Biol. 233, 70–72

    Google Scholar 

  • Holmgren, P. (1980): Variation in histone characteristics among different Drosophila chromatin types. Acta Biol. Yugoslovice, Genetika, Suppl. II, 31

    Google Scholar 

  • Keyl, H.-G. (1965): Duplikationen von Untereinheiten der chromosomalen DNS während der Evolution von Chironomus thummi. Chromosoma 17, 139–180

    Google Scholar 

  • Kram, R.; Botchan, M.; Hearst, J.E. (1972): Arrangement of highly reiterated DNA sequences in the centric heterochromatin of Drosophila melanogaster. Evidence for interspered spacer DNA. J. Mol. Biol. 64, 103–117

    Google Scholar 

  • Laird, C.D.; McCarthy, B.J. (1968 a): Nucleotide sequence homology within the genome of Drosophila melanogaster. Genetics 60, 323–334

    Google Scholar 

  • Laird, C.D. and McCarthy, B.J. (1968a): Magnitude of interspecific nucleotide sequence variability in Drosophila. Genetics 60, 303–322

    Google Scholar 

  • Laird, C.D. (1969): Molecular characterization of the Drosophila genome. Genetics 63, 865–882

    Google Scholar 

  • Lakhotia, S.C. (1974): EM autoradiographic studies on polytene nuclei of Drosophila melanogaster 3: Localization of non-replicating chromatin in the chromocentre heterochromatin. Chromosoma 46, 145–159

    Google Scholar 

  • Lakhotia, S.C.; Roy, S. (1979): Replication in Drosophila chromosomes. 1: Replication of intranucleolar DNA in polytene cells of Drosophila nasuta. J. Cell Sci. 36, 185–197

    Google Scholar 

  • Lee, C.S.; Thomas Jr., C.A. (1973): Formation of rings from Drosophila DNA fragments. J. Mol. Biol. 77, 25–55

    Google Scholar 

  • Mathews, H.R.; Bradbury, E.M. (1978): The role of H1 histone phosphorylation in the cell cycle. Expl. Cell Res. 111, 343–351

    Google Scholar 

  • Nagl, W. (1976): DNA endoreduplication and polyteny understood as evolutionary strategies. Nature 261, 614–615

    Google Scholar 

  • Pardue, M.L.; Weinberg, E.; Kedes, L.H.; Birnstiel, M.L. (1972): Localization of sequences coding for histone messenger RNA in the chromosomes of Drosophila melanogaster. J. Cell Biol. 55, 199

    Google Scholar 

  • Peacock, W.J.; Brutlag, D.; Goldring, E.; Appels, R.; Hinton, C.W.; Lindsley, D.L. (1973): The organization of highly repeated DNA sequences in Drosophila melanogaster chromosomes. Cold Spring Harbor Symp. Quant. Biol. 38, 405–416

    Google Scholar 

  • Peacock, W.J.; Lohe, A.R.; Gerlach, W.L.; Dunsmuir, P.; Dennis, E.S.; Apples, P. (1978): Fine structure and evolution of DNA in heterochromatin. Cold Spring Harbor Symp. Quant. Biol. 42, 1121–1135

    Google Scholar 

  • Perdrix-Gillot, S. (1979): DNA synthesis and endomitoses in the giant nuclei of the silkgland of Bombyx mori. Biochimie 61, 171–204

    Google Scholar 

  • Perreault, W.J.; Kaufmann, B.W.; Gay, H. (1968): Similarity in base composition of heterochromatic and euchromatic DNA in Drosophila melanogaster. Genetics 60, 289–301

    Google Scholar 

  • Perreault, W.J.; Kaufmann, B.W.; Gay, H. (1973): Repeated DNA sequences in the heterochromatic Y chromosome of adult Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 70, 773–777

    Google Scholar 

  • Rasch, E.M. (1970): DNA photocytometry of salivary gland nuclei and other tissue systems in dipteran larvae. Wied & Bahr: Introduction to quantitative cytochemistry. 2, 357–397

    Google Scholar 

  • Renkawitz-Pohl, R. (1977): No underreplication of the repetitive euchromatic 5S RNA genes during endomitosis in Drosophila hydei. J. Cell Biol. 75, 123

    Google Scholar 

  • Renkawitz-Pohl, R. (1978): Number of the repetitive euchromatic 5S RNA genes in polyploid tissues of Drosophila hydei. Chromosoma 66, 249–258

    Google Scholar 

  • Renkawitz-Pohl, R. (1979): Proportional polyploidization of 5S RNA genes in the ovary of Drosophila melanogaster containing three 5S RNA gene loci. Chromosoma 70, 305–312

    Google Scholar 

  • Renkawitz-Pohl, R.; Kunz, W. (1975): Underreplication of satellite DNAs in polyploid ovarian tissue of Drosophila virilis. Chromosoma 49, 375–382

    Google Scholar 

  • Risler, H. (1959): Polyploide und somalische Reduktion in der Larvenepidermis von Aedes aegypti (Culicidae). Chromosoma 10, 184–209

    Google Scholar 

  • Risler, H. (1961): Untersuchungen zur somalischen Reduktion in der Metamorphose des Stechmückendarms. Biol. Zentralbl. 80, 413–428

    Google Scholar 

  • Ritossa, F.M.; Spiegelmann, S. (1965): Localization of DNA complementary to rRNA in the nucleus organizer region of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 53, 737–745

    Google Scholar 

  • Rodman, T.C. (1967): DNA replication in salivary gland nuclei of Drosophila melanogaster at successive larval and prepupal stages. Genetics 55, 375–386

    Google Scholar 

  • Rudkin, G.T. (1964): The structure and function of heterochromatin. Proc. 11th Intern. Congr. Genetics 2, 359–374

    Google Scholar 

  • Rudkin, G.T. (1965): Non replicating DNA in giant chromosomes. Genetics 52, 470

    Google Scholar 

  • Rudkin, G.T. (1969): Non replicating DNA in Drosophila. Genetics 61, Suppl. 1, 227–228

    Google Scholar 

  • Rudkin, G.T. (1972): Replication in polytene chromosomes. In: Developmental studies on giant chromosomes, (ed. Beermann, W.), pp. 59–85. Berlin, Heidelberg, New York: Springer

    Google Scholar 

  • Rudkin, G.T.; Schulz J. (1961): Disproportional synthesis of DNA in polytene chromosome regions in Drosophila melanogaster. Genetics 46, 893–894

    Google Scholar 

  • Schachat, F.H.; Hogness D.S. (1973): Repetitive sequences in isolated Thomas circles from Drosophila melanogaster. Cold Spring Harbor Symp. Quant. Biol. 38, 371–381

    Google Scholar 

  • Schmidt, E.R. (1980): Two AT-rich satellite DNAs in the Chironomid Glyptotendipes barbipes (Staeger). Isolation and localization in polytene chromosomes of G. barbipes and Chironomus thummi. Chromosoma 79, 315–328

    Google Scholar 

  • Schmidt, E.R.; Vistorin, G.; Keyl, G.-H. (1980): An AT-rich DNA component in the genomes of Chironomus thummi and Chironomus piger. Chromosoma 76, 35–45

    Google Scholar 

  • Skinner, D.M.; Triplett, L.L. (1967): The selective loss of DNA satellites on deproteinization with phenol. Biochem. Biophys. Res. Commun. 28, 892–897

    Google Scholar 

  • Spear, B.B. (1974): The genes for ribosomal RNA in diploid polytene chromosomes of Drosophila melanogaster. Chromosoma 48, 159–179

    Google Scholar 

  • Spear, B.B. (1977): Differential replication of DNA sequences in Drosophila chromosomes. Am. Zool. 17, 695–706

    Google Scholar 

  • Spear, B.B.; Gall, J.G. (1973): Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 70, 1359–1363

    Google Scholar 

  • Steinemann, M. (1978): Co-replication of satellite DNA of Chironomus melanotus with mainband DNA during polytenization. Chromosoma 66, 127–139

    Google Scholar 

  • Swift, H.; Rasch, E.M. (1954): Nucleoproteins in Drosophila polytene chromosomes. J. Histochem. Cytochem. 2, 456–457

    Google Scholar 

  • Walter, L. (1973): Syntheseprozesse an den Riesenchromosomen von Glyptotendipes. Chromosoma 41, 327–360

    Google Scholar 

  • Wollenzien, P.; Barsanti, P.; Hearst, J.E. (1977): Location and underreplication of satellite DNA in Drosophila melanogaster. Genetics 87, 51–65

    Google Scholar 

  • Woodcock, D.M.; Sibatani, A. (1975): Differential variations in the DNA of Drosophila melanogaster during development. Chromosoma 50, 147–173

    Google Scholar 

  • Yumis, J.J.; Yasmineh, W.G. (1971): Heterochromatin, satellite DNA, and cell function. Science 174, 1200–1209

    Google Scholar 

  • Zuchowski, Ch.I.; Harford, A.G. (1976): Unintegrated ribosomal genes in diploid and polytene tissues of Drosophila melanogaster. Chromosoma 58, 235–246

    Google Scholar 

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  1. Institut für Entwicklungsphysiologie der Universität Köln, Köln, Federal Republic of Germany

    L. Dennhöfer

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Communicated by H. F. Linskens

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Dennhöfer, L. Underreplication during polytenization?. Theoret. Appl. Genetics 63, 193–199 (1982). https://doi.org/10.1007/BF00303991

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