Electronic Resource
Springer
Theoretical and applied genetics
63 (1982), S. 193-199
ISSN:
1432-2242
Keywords:
Drosophila
;
Polytene nuclei
;
Underreplication
;
Polytenization
;
Cytophotometry
;
Heterochromatin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Recent cytophotometric DNA determinations and results of labeling experiments are compared with results of biochemical experiments concerning larval polytene salivary gland nuclei of Drosophila melanogaster. Recent publications (Dennhöfer 1981; 1982 a, b) demonstrate that methodological errors both in hydrolysis of the DNA before Feulgen reaction and in interpretation of the cytophotometric values give raise to the hypothesis of heterochromatic underreplication during polytenization. It is concluded also that methodological difficulties cause the absence of polytene SAT-DNA in biochemical centrifugation experiments since, because of different solubilities of eu- and heterochromatic DNA, the latter is not resolved in DNA isolation procedures from polytene nuclei.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00303991
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