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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 63 (1982), S. 193-199 
    ISSN: 1432-2242
    Keywords: Drosophila ; Polytene nuclei ; Underreplication ; Polytenization ; Cytophotometry ; Heterochromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recent cytophotometric DNA determinations and results of labeling experiments are compared with results of biochemical experiments concerning larval polytene salivary gland nuclei of Drosophila melanogaster. Recent publications (Dennhöfer 1981; 1982 a, b) demonstrate that methodological errors both in hydrolysis of the DNA before Feulgen reaction and in interpretation of the cytophotometric values give raise to the hypothesis of heterochromatic underreplication during polytenization. It is concluded also that methodological difficulties cause the absence of polytene SAT-DNA in biochemical centrifugation experiments since, because of different solubilities of eu- and heterochromatic DNA, the latter is not resolved in DNA isolation procedures from polytene nuclei.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 45 (1974), S. 29-38 
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The map of larval salivary gland chromosomes of the mosquitoCulex pipiens L. is amended as follows: The banding pattern is corrected in arm 1L, and completed in 2L. The amendments were verified on isolated entire chromosomes. Discrepancies between the three maps published previously, are ascribed to differences in method of the respective authors. In map collation, only well-spread chromosomes of a similar degree of polyteny should be used. The spatial arrangement of chromosomes in the nucleus is discussed, and indications are given for distinguishing between chromosome ends and accidental breaks.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 45 (1975), S. 163-175 
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the salivary gland chromosomes of the mosquitoCulex pipiens L. band 10 C 3 of the small chromosome I in female larvae always shows a homozygous heterochromatic “balloon”, while male larvae are heterozygous for this structure, the homologous band being euchromatic. In males, heterozygous for a male-linked reciprocal translocation, the chromosome with the distinct euchromatic band is always involved in the segmental interchange with an autosome. It is concluded that the euchromatic state of band 10 C 3 represents the male-determining allelomorph, and the heterochromatic state the female-determining allelomorph (M and m, respectively). C. pipiens thus appears to be an excellent example of diplogenotypic determination of sex where the sexes are different in one pair of allelomorphs only. At the chromosomal level, this sexual dimorphism is expressed as a heterochromatic versus an euchromatic state of a single band. The significance of this observation is discussed in connection with the problem of the evolution of sex-determining mechanisms within the Nematocera.
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  • 4
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Genetica 45 (1974), S. 39-59 
    ISSN: 1573-6857
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fifteen X-ray induced chromosomal aberrations in twelve autogenous lines of the mosquitoCulex pipiens were analysed: eight simple reciprocal translocations, one double reciprocal translocation, one translocation combined with an intrachromosomal transposition and a small deficiency, one simple transposition, and one pericentric inversion. The breakpoints were located on the morphological map of the salivary gland chromosomes. Their distribution seems to be random. A consideration of distances between breakpoints argues against the assumption of a linear order of chromosomes in the elongated sperm head. The extent of sterility in the heterozygote was recorded for 12 of the aberrations. Translocation heterozygotes, carrying the genetic pool of the lines here analysed, produced about 50% aneuploid gametes, independently of the length of the interstitiell segments.
    Type of Medium: Electronic Resource
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