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  • Immunocytochemistry  (62)
  • Lepidoptera  (58)
  • Springer  (120)
  • 2015-2019
  • 1980-1984  (120)
  • 1925-1929
  • 1983  (62)
  • 1981  (58)
Collection
Keywords
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  • Springer  (120)
Years
  • 2015-2019
  • 1980-1984  (120)
  • 1925-1929
Year
  • 1
    Electronic Resource
    Electronic Resource
    On the cellular localization of phosphoenolpyruvate carboxylase in Sorghum leaves (1981)
    Springer
    Planta 151 (1981), S. 226-231 
    Details
    ISSN: 1432-2048
    Keywords: Immunocytochemistry ; (PEP carboxylase) ; PEP carboxylase ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395173
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  • 2
    Electronic Resource
    Electronic Resource
    Immunocytochemical location of vitellin in the egg of the silkworm,Bombyx mori, during early developmental stages (1983)
    Springer
    Development genes and evolution 192 (1983), S. 113-119 
    Details
    ISSN: 1432-041X
    Keywords: Vitellin ; Yolk granule ; Yolk protein ; Silkworm ; Embryogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitellin was purified from eggs of the silkworm,Bombyx mori, by a new method in which vitellin was extracted from isolated yolk granules. The purified vitellin had a molecular weight of 540,000. An antibody against purified vitellin was prepared in rabbits. It reacted with the hemolymph vitellogenin as well as with purified vitellin, but not with other proteins in the hemolymph or in the extract from yolk granules. The anti-vitellin IgG was used to immunocytochemically locate vitellin in theBombyx non-diapause egg during early developmental stages. In the egg, just after oviposition, vitellin was located in internal yolk granules and in small yolk granules of the periplasm. During the early developmental stages studied, vitellin was not metabolized uniformly throughout the egg. The vitellin of the internal yolk granules located at the posterior-dorsal part and of the small peripheral yolk granules was utilized in 16 h and 2 days, respectively, after oviposition. A thin, very vitellin-poor layer was located between the periplasm and the vitellin-rich interior in the newly laid egg. it was always in close contact with the periphery where blastoderm and germ-band cells developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848679
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  • 3
    Electronic Resource
    Electronic Resource
    Sex pheromone of the cone pyralid Dioryctria abietella (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 20-26 
    Details
    ISSN: 1570-7458
    Keywords: Dioryctria abietella ; Cone pyralid ; Lepidoptera ; Pyralidae ; Sex pheromone, (Z,E)-9,11-tetradecadienyl acetate ; Single sensillum recordings ; Electroantennography ; Gas chromatography ; Mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'analyse en chromatographie gazeuse associée à une détection électroantennographique a montré que l'acétate (Z,E)-9,11-tétradécadiényle (Z,E)-9,11–14:Ac est l'un des composants de la phéromone de Dioryctria abietella Schiff (Lepid.: Pyralidae). Couplage chromatographie en phase gazeuse spectrometrie de masse a montré la présence d'acétate tétradécadiényle avec un spectre de masse et un indice de rétention identiques au Z,E-9,11–14:Ac Un récepteur cellulaire sensible à la fois au Z,E-9,11–14:Ac et à un extrait de la femelle a été identifié sous l'antenne du mâle. Les analyses des antennogrammes et de la cellule isolée ont étayé la caractérisation du composant de la phéromone comme étant Z,E-9,11–14:Ac. Un récepteur cellulaire additionnel sensible à l'acétate (Z.)-9-tétradécadiényle et à l'acétate (Z.E.)-9,12-tétradécadiényle a été trouvé sur l'antenne du mâle, mais il n'était pas activé par l'extrait de la femelle. Sur le terrain Z,E-9,11–14:Ac, présenté seul, attirait des nombres importants de mâles de D. abietella. L'addition de l'acétate (Z)-9-tétradécényle a inhibé l'attraction des mâles par les pièges.
    Notes: Summary Gas chromatographic analyses coupled with electro-antennographic detection indicated that (Z,E)-9,11-tetradecadienyl acetate (Z,E-9, 11–14:Ac) is a pheromone component of the cone pyralid Dioryctria abietella. Gas chromatographic-mass spectrometric analyses confirmed the presence of a tetradecadienyl acetate with mass spectrum and retention index identical to Z,E-9,11–14:Ac. A receptor cell sensitive to both Z,E-9,11–14:Ac and the female extract was identified on the male antenna. An additional receptor cell sensitive to (Z)-9-tetradecenyl acetate and (Z,E)-9,12-tetradecadienyl acetate was found on the male antenna but was not activated by the female extract. In the field Z,E-9,11–14:Ac presented alone attracted significant numbers of male D. abietella. Addition of (Z)-9-tetradecenyl acetate inhibited the attraction of males to traps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300895
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  • 4
    Electronic Resource
    Electronic Resource
    Suitability of selected leguminous plants for development of Maruca testulalis larvae (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 174-178 
    Details
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Pyralidae ; Maruca testulalis ; Pod borer ; Development ; Nutritional suitability ; Host plant ; Crotalaria ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les possibilités de développement larvaire de Maruca testulalis sur les fleurs de huit espèces végétales ont été examinées en laboratoire. La comparaison a porté sur Crotalaria retusa, C. juncea, C. saltiana, C. misereniensis, C. mucronata, C. amazonas, Cajanus cajan et l'hôte principal de cette chenille, Vigna unguiculata. En tenant compte de la mortalité larvaire et des indices de croissance (G.I.), ces plantes ont été divisées en trois catégories principales: 1 celles provoquant une mortalité larvaire de 0–30% et ayant des G.I. ≥60%, constituent les plantes hôtes convenables (Vigna unguiculata seule); 2 les plantes qui provoquent une mortalité larvaire de 30≤50% et ont des G.I. de 30 à 60% de la plante hôte principale (Cajanus cajan, Crotalaria amazonas, C. saltiana, C. mucronata; 3 les plantes qui causent 50–100% de mortalité larvaire et dont les G.I. sont inférieurs à 30% de la plante hôte principale (Crotalaria retusa, C. juncea, C. misereniensis). Les résultats déjà publiés sur les choix du lieu de ponte des femelles et l'utilisation de C. juncea comme plante piège, sont discutés à la lumière de ces données nouvelles.
    Notes: Abstract Flowers of eight plant species were evaluated under laboratory conditions for their suitability as larval growth media for the cowpea pod borer, Maruca testulalis. The plants tested were Crotalaria retusa, C. juncea, C. saltiana, C. misereniensis, C. amazonas, Cajanus cajan and the principal host of the borer, Vigna unguiculata (cowpea), was included for comparison. Based on the data obtained on larval mortality and growth indices (GI) the plants were divided into 3 categories namely: I: Those causing 0–30% mortality and having GI value ≥60% form suitable host plants. This group only included V. unguiculata. — II: Those plant species causing 30≤50% larval mortality and having GI value 30%≤60% of the principal host plant (Cajanus cajan, Crotalaria amazonas, C. saltiana, C. mucronata). This group of species is marginally suitable as hosts. — III: Plants causing 50–100% larval mortality and having GI value ≤30% of principal host plant (C. retusa, C. juncea and C. misereniensis). Previously published data on the oviposition preference of the adult moth are discussed in the light of the present findings and the use of C. juncea as a possible trap crop.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338666
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  • 5
    Electronic Resource
    Electronic Resource
    Food plant specialization and feeding efficiency in the tent caterpillars Malacosoma disstria and M. americanum (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 106-110 
    Details
    ISSN: 1570-7458
    Keywords: assimilation efficiency ; growth efficiency ; niche breadth ; specialization ; hostplant relations ; plant-insect interactions ; Malacosoma americanum ; Malacosoma disstria ; Lasiocampidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Raupen von Malacosoma americanum, einer oligophagen Art, die sich vor allem auf Prunus und andern baumartigen Rosaceen. entwickelt, wurden mit Raupen der polyphageren Verwandten M. disstria in Hinblick auf die Verwertung der Blätter ihres gemeinsames Wirtes Prunus serotina verglichen. Wirerhielten ähnliche Werte wie sie früher für andere Lepidopteren publiziert worden waren und zwar in Bezug auf die üblichen Messwerte, Anteil verwertete Nahrung, Wachstum in Trockengewicht pro Einheit gefressene oder verwertete Nahrung. Zudem unterschieden sich die beiden Arten in keiner Masszahl für Effizienz. Unsere Resultate sind im Einklang mit der Folgerung, das spezialisierte phytophage Insekten ihre Wirtspflanzen nicht besser ausnützen als as polyphage Arten tun.
    Notes: Abstract Larvae of Malacosoma americanum (F.) an oligophagous species that feeds primarily on Prunus and other rosaceous trees, were compared to larvae of the more highly polyphagous congener M. disstria Hb., with respect to the efficiency of utilization of the foliage of a common host plant, Prunus serotina Ehrh. We obtained values similar to those reported for other Lepidoptera for the commonly used measures of the fraction of ingested food that was assimilated, and for the growth in dry weight per unit of food ingested or assimilated. Moreover, the two species did not differ in any measure of efficiency. Our results are compatible with the conclusion that specialized phytophagous insects do not use their host plants with greater physiological efficiency than do generalized insects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300873
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  • 6
    Electronic Resource
    Electronic Resource
    Sex attractants for clearwing moths: Synanthedon vespiformis and Chamaesphecia tenthrediniformis (and/or C. empiformis) (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 203-205 
    Details
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Sesiidae ; Synanthedon vespiformis ; Paranthrene tabaniformis ; Chamaesphecia empiformis ; Chamaesphecia tenthrediniformis ; Attractants ; Pheromones ; Clearwing moths ; Nemapogon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338671
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  • 7
    Electronic Resource
    Electronic Resource
    Precocious metamorphosis and moulting deficiencies induced by an anti-JH compound, FMev in the fall webworm, Hyphantria cunea (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 65-70 
    Details
    ISSN: 1570-7458
    Keywords: Insect growth regulators ; Anti-juvenile hormone ; Fluoromevalonate ; Precocious metamorphosis ; Premature pupation ; Ecdysis ; Fall webworm ; Hyphantria cunea ; Lepidoptera ; Arctiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Fluormevalosäure (FMev), bekannt als ein spezifischer Inhibitor der Biosynthese des Juvenilhormons (JH) in Insekten, wurde in 0,1–50 μg/Tier Dosis topikal an 3., 4. und 5. Larvenstadien von Hyphantria cunea getestet. Die Raupen wurden auf einem semi-synthetischen, künstlichen Nährboden bei 25° und unter Langtagsbedingungen (18 : 6 St., Licht/Dunkel) gezüchtet. Diese Verbindung rief drei verschiedene Typen spezifischer Reaktionen hervor: 1) verfrühte Metamorphose, 2) gehemmte Häutung und 3) verlängerte Larvenentwicklung. Vor der verfrühten Verpuppung wurde normales Verhalten beobachtet. Die Larven des 3. oder 4. Stadiums häuteten sich meist erst nach einem interkalaren Larvenstadium in verfrühte Puppen. Unter den drei Larvenstadien erwies sich das 5. Stadium gegen die Anti-JH-Verbindung am empfindlichsten. In allen getesteten Entwicklungsphasen wiesen die frisch gehäuteten Larven die höchste Empfindlichkeit gegen FMev auf. Nach der Häutung wurde stufenweises Absinken der FMev-Empfindlichkeit beobachtet, im 5. Larvenstadium verursachte die Verbindung jedoch selbst am letzten Tag des Wachstums zu einen relativ hohen Prozentzahl verfrühte Verpuppung. Eine zweite typische Wirkung von FMev war die Hemmung der Häutungsprozesse. Zwei grundlegende Stufen der Häutungsstörungen unterschieden sich voneinander: 1) Als Folge der Anwendung hoher Dosen von FMev konnte die Mehrzahl der Raupen die alte Larvenkutikula nicht öffnen und ging deswegen in kurzer Zeit zugrunde. 2) Bei Behandlung mit niedrigen Dosen der Anti-JH-Verbindung häuteten sich einige Hyphantria-Larven scheinbar normal; nach der Häutung waren aber alle unfähig, die normalen Bewegungen und die Nahrungsaufnahme fortzusetzen. Auch die vorzeitigen Puppenhäutungen wurden in meisten Fällen durch die Anti-JH-Behandlung gehemmt. Die morphogenetischen Wirkungen von FMev konnten durch eine topikale Behandlung mit Hydroprene, einem hochaktiven JH-Analogen, vollständig oder teilweise verhindert werden.
    Notes: Summary Fluoromevalonate (FMev, ZR-3516) known as an inhibitor of JH biosynthesis was topically applied in 0.1 to 50 μg/specimen doses to the 3rd, 4th, and 5th instar caterpillars of Hyphantria cunea Drury. The anti-JH compound induced 3 main types of specific responses: 1) precocious metamorphosis, 2) inhibition of ecdysis, and 3) prolongation of larval development. Precocious pupation was accompanied by behavioural events typical of normal pupation. Third and 4th instar larvae metamorphosed prematurely mostly with the intervention of an intercalary larval instar. The 5th instar exhibited the highest sensitivity to the anti-JH agent. Within each larval stage the freshly moulted insects proved to be the most susceptible to FMev. Afterwards, the incidence of morphogenetic reaction gradually decreased with age. In another fraction of Hyphantria larvae not responding with precocious pupation, FMev evoked varying degrees of ecdysial disturbance which always resulted in the death of caterpillars. In most cases the anti-JH compound inhibited the premature pupal moult, too, and these affected insects died as tanned pharate pupae. A complete or partial “rescue” from the effects of FMev was elicited, if simultaneously or subsequently, a single topical dose of a JH analogue, hydroprene was also administered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300901
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  • 8
    Electronic Resource
    Electronic Resource
    The effect of variation in host plant on the growth of an oligophagous insect, Malacosoma americanum and its polyphagous relative, Malacosoma disstria (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 163-168 
    Details
    ISSN: 1570-7458
    Keywords: host-plant relations ; host variation ; Malacosoma americanum ; Malacosoma disstria ; Lasiocampidae ; Geometridae ; Lepidoptera ; niche breadth ; specialization ; plantinsect interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Raupen von Malacosoma americanum (F.), einer oligophagen Art, die sich vor allem auf Prunus und andern baumartigen Rosaceen entwickelt, wurden verglichen mit Raupen der polyphageren Verwandten M. disstria Hb. und zwar im Hinblick auf deren Empfindlichkeit auf Unterschiede im Blatt ihrer gemeinsamen Wirtspflanze, Prunus serotina Ehrh. Das Puppengewicht und die Entwicklungszeit bis zur Verpuppung wurden gemessen bei Raupen, welche auf Blättern von freiwachsenden und von beschatteten Jungpflanzen gezüchtet worden waren. Die Blattunterschiede hatten eine ausgesprochene Wirkung, aber es gab keine Unterschiede in der Reaktion der beiden Arten. Dieser Vergleich lässt vermuten, dass die Empfindlichkeit auf intraspezifische Unterschiede der Wirtspflanzenqualität bei wirtsspezifischen und polyphagen Arten gleich ist. Indessen dürften sich laut anderen Resultaten einige Insektenarten anders verhalten.
    Notes: Abstract Larvae of Malacosoma americanum (F.)(Lepidoptera: Lasiocampidae) an oligophagous species that feeds primarily on Prunus and other rosaceous trees, were compared to larvae of the more highly polyphagous congener M. disstria Hb., with respect to their sensitivity to variation in the foliage of a common host plant, Prunus serotina Ehrh. Pupal weight and time to pupation were measured on larvae reared on foliage from open-grown and from shaded saplings. The difference in foliage had a pronounced effect, but no difference was evident between the species in their response to the variation in foliage. This comparison implies that sensitivity to intraspecific variation in host quality does not differ between host-specific and generalized species. However, results from other species suggest that some species of insects do differ in this respect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300882
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  • 9
    Electronic Resource
    Electronic Resource
    Host races and sympatric speciation in small ermine moths, Yponomeutidae (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 280-292 
    Details
    ISSN: 1570-7458
    Keywords: Allozyme variation ; Lepidoptera ; Yponomeuta padellus ; evolutionary stages ; F-statistics ; panmixis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Le degré de différenciation génétique en allozymes à des niveaux divers de divergence évolutive (populations conspécifiques, sibling species et non-sibling species) d'Yponomeuta a été déterminé. Les distances génétiques entre des sibling species ou des non-sibling species sont très larges. Il en est conclu que de telles estimations ne fournissent pas beaucoup d'informations sur le phénomène de spéciation même. Des coéfficients de reproduction consanguine (F ST) ont été calculés pour des populations d'Y. padellus provenant de six plantes-hôtes. La valeur moyenne F STest .030±.012. Les valuers de F ST des populations recueillies sur Crataegus, (plante-hôte habituelle d'Y. padellus), sont inférieures 2–3.5 fois à celles des populations de l'ensemble des autres plantes-hôtes. L'apparition de races en fonction de l'hôte, mesurée par les différences importantes dans de fréquences des allozymes entre populations sympatriques sur plusieurs plantes-hôtes, a été examinée dans quatre régions. Il apparaît ainsi que la formation de races en fonction de l'hôte se produit chez Y. padellus et que la spéciation sympatrique est un évènement très vraisemblable.
    Notes: Abstract The amount of genetic differentiation at various levels of evolutionary divergence (conspecific populations, sibling species and non-sibling species) in Yponomeuta was determined. Genetic distances between siblings or non-siblings were found to cover a wide range. It is concluded that such estimates do not give much information on the speciation process itself. Inbreeding coefficients were calculated for populations of Y. padellus from a total of six host plants. The grand mean F ST value is 0.030±0.012. F ST values for populations sampled from Crataegus, the common food plant of Y. padellus, are 2–3.5 times smaller than those for populations from the other food plants taken together. Host race formation, as measured by significant differences in allozyme frequencies between sympatric populations on two or more food plants, was investigated in four areas. Host race formation seems to occur in Y. padellus and sympatric speciation is a likely event.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00347708
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  • 10
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    Development of artificial diets for laboratory rearing of larvae of the spiny bollworm, Earias insulana (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 121-122 
    Details
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Noctuidae ; Earias insulana ; Bollworm ; Artificial diets ; Insect fecundity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300911
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  • 11
    Electronic Resource
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    Repellency of pheromones released by females of Heliothis armigera and H. zea to females of both species (1981)
    Springer
    Entomologia experimentalis et applicata 30 (1981), S. 123-127 
    Details
    ISSN: 1570-7458
    Keywords: Sex Pheromones ; Repellent ; Heliothis armigera ; Heliothis zea ; Lepidoptera ; Noctuidae ; cotton bollworm ; corn earworm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'examen en olfactomètre a porté sur les réactions face à d'autres femelles de la même espèce, de femelles vierges ou ayant copulé d'Heliothis armigera Hübner et H. zea Boddie. Le lot comprenait 8 femelles, vierges ou ayant copulé en présence d'une femelle vierge ou ayant copulé. Les 4 combinaisons possibles de femelles vierges et de femelles ayant copulé ont été examinées avec 12 répétitions pour chaque espèce. Un extrait de l'extrémité de l'abdomen de femelles vierges d'une espèce a été présenté aux femelles de l'autre espèce pour examiner les possibilités de réactions interspécifiques aux phéromones. Pour chaque espèce, les réactions interspécifiques de répulsion entre femelles ont été hautement significatives par rapport aux témoins, à l'exception toutefois des réactions de femelle ayant copulé face à des femelles ayant elles aussi copulé. Les répulsions moyennes chez H. armigera et H. zea pour les 8 femelles de chaque expérience ont été: a) vierges en présence d'une vierge: 7,33 et 7,66; b) vierges en présence d'une femelles ayant copulé: 5,76 et 5,58; c) femelles ayant copulé en présence d'une vierge: 4,67 et 4,83. Les différences sont hautement significatives entre chaque paire de moyennes et entre chaque paire et le lot témoin; 3,17; 3,17; 3,42; 4,00 pour H. armigera; 3,17; 3,50; 2,83 et 3,75 pour H. zea. Les femelles vierges des deux espèces, H. armigera et H. zea ont présenté une réaction de répulsion en présence d'un extrait de l'abdomen de l'autre espèce; les répulsions moyennes étant respectivement 5,53 et 5,33 contre 3,83 et 3,58 pour le lot trémoin. On peut en conclure que ces répulsions doivent entraîner une tendance à la répartition uniforme.
    Notes: Abstract An olfactometer was used to determine the effect of pheromones released by females of the bollworms Heliothis armigera (Hübner) and H. zea (Boddie) on females of the same species. Four combinations of virgin and mated females were tested for repellency of one to the other. Evidence is presented that females of the two bollworms were repelled by females of the same species. In addition, extracts of virgin female abdomens of each species repelled virgin females of the other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300876
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  • 12
    Electronic Resource
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    Multivoltinism in Apanteles bignellii and the influence of weather on synchronisation with its host Euphydryas aurinia (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 155-162 
    Details
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Braconidae ; Lepidoptera ; Nymphalidae ; Apanteles bignellii ; Euphydryas aurinia ; Multivoltinism ; Synchronisation ; Weather
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung In einer Population von Euphydryas aurinia (Rottemburg) bei Oxford, England treten während einer Generation von E. aurinia drei Normalgenerationen von Apanteles bignellii Marshall auf. Jede Generation des Parasitoids kann charakterisiert werden durch das befallende Wirtsstadium und durch die aus einem Wirt schlüpfende Zahl Parasitoide. Parasitoide schlüpfen in Normalgenerationen aus dem 3., dem 4. und dem 6. Stadium des Wirts; in Ausnahmegenerationen schlüpfen sie aus dem 2. und 5. Stadium. Bis zu 70 Parasitoide können aus einer Altraupe (6. Stadium) schlüpfen und die Dauer dieses Stadiums kann bis auf 2 Wochen verlängert werden. Die Ausnahmegenerationen von A. bignellii aus Zweitlarven dürften aus Eiablagen in frühe Erstlarven stammen. Fünftlarven des Wirts, aus denen Parasitoide schlüpfen, sind ungewöhnlich klein und fressen nicht; sie dürften das Resultat sein eines Uebermasses von Apenteles-Eiern, die in frühe Viertlarven gelegt wurden. Die Synchronisation zwischen dem Parasitoiden und dem Wirt während der Zeit, da E. aurinia im Puppen-, Adult- oder Eistadium ist, wird aufrechterhalten durch ein verlängertes Coconstadium von Apanteles. Die Puppen des Parasitoiden entwickeln sich normal und die Adulten schlüpfen, bleiben aber bis 4 Wochen lang im Cocon, bevor sie sich eine Ausgangsöffnung machen. Das Wetter kann den Parasitierungsgrad der letzten Wirtsstadien beeinflussen. Wenn der Frühling kalt ist mit klarem Himmel, kann die Synchronisierung zwischen Parasitoiden, die aus Viertlarven des Wirts schlüpfen und potentiellen Fünft- und Sechtstlarven des Wirts schlecht werden. Die Entwicklung von Apanteles-Puppen wird durch die Umgebungstemperatur beeinflusst, während E. aurinia-Larven ihre Temperatur erhöhen, indem sie sich sonnen und deshalb rasch wachsen. Wenn die Parasitoiden unter solchen Bedingungen schlüpfen, sind die meisten potentiellen Wirte schon verpuppt und damit nicht mehr geeignet für die Parasitierung. Die Mechanismen der Synchronisation und der Wettereinfluss auf diese Vorgänge wird diskutiert.
    Notes: Abstract The gregarious endoparasite, Apanteles bignellii Marshall is specific to the nymphalid butterfly, Euphydryas aurinia (Rottemburg) in the British Isles. The synchronisation between host and parasitoid is described at a site near Oxford, England where both occur. Three regular generations of A. bignellii occur in one generation of the host in the studied population. Relevant features of the biology of A. bignellii and E. aurinia are described, including a method of distinguishing the number of Apanteles larval instars based upon shed cuticle remnants. Mechanisms for host-parasitoid synchronisation are outlined, especially a protracted parasitoid cocoon stage when the host is unavailable for attack during the chrysalis, adult and egg stages. Cool, but sunny weather conditions in spring can influence the degree of parasitisation experienced by final instar host caterpillars. The timing of adult A. bignellii emergence and subsequent attack on early instar hosts can lead to additional, partial, generations of parasitoids from second and fifth instar hosts.
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    Synthesis of sex pheromone components of the forest tent caterpillar,Malacosoma disstria (Hübner) and of the western tent caterpillar,Malacosoma californicum (Packard) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 159-164 
    Details
    ISSN: 1573-1561
    Keywords: Synthesis ; isomers ; 5,7-dodecadienal ; sex pheromone ; Malacosoma disstria ; Malacosoma californicum ; Lepidoptera ; Lasiocampidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract All four geometrical isomers of 5,7-dodecadien-1-ol have been stereoselectively synthesized by using Wittig condensation reactions. (5 Z,7E)-5,7-Dodecadien-1-ol and its corresponding aldehyde are components of the sex pheromone of the forest tent caterpillar. (5 E,7 Z)-5,7-Dodecadienal is a component of the pheromone of the western tent caterpillar. These compounds have been successfully tested in the field.
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    URL: http://dx.doi.org/10.1007/BF00988643
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    Feeding preferences of spruce budworm (Choristoneura fumiferana Clem.) larvae to some host-plant chemicals (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 391-402 
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    ISSN: 1573-1561
    Keywords: Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; Abies balsamea ; feeding stimulants ; feeding deterrents ; feeding preference ; sugars ; glycosides ; amino acids ; organic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Single-choice and three-choice tests were used to determine the relative importance of host-plant chemical extracts in eliciting feeding by spruce bud worm larvae. Water-soluble components of the host trees are the most important and, of these, the sugar and glycoside fractions are the most stimulating. The amino acid and organic base fractions have no apparent effect. The organic acid fractions deter feeding slightly.
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    2-Furaldehyde from baldcypress (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 89-101 
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    ISSN: 1573-1561
    Keywords: Baldcypress ; Taxodium distichum ; 2-furaldehyde ; Bombyx mori ; Lepidoptera ; Bombycidae ; growth inhibition ; toxicity ; Georgia silkworm industry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract 2-Furaldehyde was growth inhibitory and toxic to larvae ofBombyx mori at concentrations as low as 1 ppm. Baldcypress,Taxodium distichum, heartwood released this volatile at concentrations in excess of these levels. The adverse effect of this chemical on silkworms may partially account for the demise of the Georgia silkworm industry (circa 1765), after the construction of a baldcypress rearing facility.
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    URL: http://dx.doi.org/10.1007/BF00988637
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    Attractant lures for males of the pea moth,Cydia nigricana (F.) Containing (E)-10-dodecen-1-yl acetate and (E,E)-8,10-dodecadien-1-yl acetate (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 563-573 
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    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Lepidoptera ; Olethreutidae ; sex attractant ; (E)-10-dodecen-1-yl acetate ; (E,E)-8 ; 10-dodecadien-1-yl acetate ; lure ; formulation ; antioxidant ; monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Lures containing different amounts of (E)-10-dodecen-1-yl acetate and (E,E)-8, 10-dodecadien-1-yl acetate were formulated in natural rubber stoppers or polyethylene vials and tested in traps in the field to establish relative attractiveness to males of the pea moth,Cydia nigricana (F.), dose-response relationships, and the effects of weathering. Initially, both formulations of (E,E)-8,10-dodecadien-1-yl acetate were much more attractive than those of (E)-10-dodecen-1-yl acetate, but their activity diminished considerably after only 5 days in the field because an inhibitor was formed, and polyethylene formulations were not attractive after one week. However, lures containing (E)-10-dodecen-1-yl acetate, with or without antioxidants, in both rubber and polyethylene were still attractive after three months' exposure in the field and are therefore suitable for practical monitoring of pea moth populations.
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    URL: http://dx.doi.org/10.1007/BF00987704
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    Air permeation tests with (Z,Z)-3,13-octadecadien-1-ol acetate for reduction in trap catch of peachtree and lesser peachtree borer moths (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 575-582 
    Details
    ISSN: 1573-1561
    Keywords: Peachtree borer ; lesser peachtree borer ; Synanthedon exitiosa ; S. pictipes ; Lepidoptera ; Sessiidae ; Z,Z-3 ; 13-octadecadien-1-ol acetate sex pheromone ; traps ; air permeation ; trap catch reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Air permeation trials were conducted to determine whether trap catches of the peachtree borer (PTB)Synanthedon exitiosa (Say) and lesser peachtree borer (LPTB)S. pictipes (Grote and Robinson) could be reduced through application of the synthetic pheromone of the PTB, (Z,Z)-3,13-octadecadien-1-ol acetate (Z,Z-ODDA), at 92% isomeric purity. The pheromone was released into the air of peach orchard test plots with hanging laminated plastic dispensers or hollow fiber dispensers in the trees and by aerial dispersal of microcapsules containing a solution of the lure. Results showed that all formulations ofZ, Z-ODDA reduced trap catches of both PTB and LPTB in treated plots relative to catches in traps in untreated plots. In one air permeation experiment, three types of laminated dispensers and one type of hollow fiber dispenser each reduced trap catch of PTB by 93–100% and of LPTB by 75–95%. In a separate test, two formulations of microencapsulatedZ,Z-ODDA did not reduce trap catch of male PTB as effectively as the laminated dispenser; results for LPTB were similar for both the dispensers and microcapsules. Catches of male PTB moths in traps baited with laminated and or fiber pheromone dispensers containing ODDA (94∶6%Z,Z∶E,Z) were not significantly different. LPTB male moths were also captured in these traps, the catches being dependent upon the initial pheromone loading and the type of dispensers.
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    URL: http://dx.doi.org/10.1007/BF00987705
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    Insect sex pheromones: Evaporation rates of alcohols and acetates from natural rubber septa (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 627-633 
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    ISSN: 1573-1561
    Keywords: Insect sex pheromones ; insect sex attractants ; Lepidoptera ; pheromones ; pheromone formulations ; insect population monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The half-lives (t 1/2) of alcohol sex pheromones, 1-alkanols, acetate sex pheromones, and an epoxide (disparlure) were determined on natural rubber septa. Thet 1/2 values for the homologous alcohols from decanol to heptadecanol increased regularly from 2.2 to 1117 days, but thet 1/2 of octadecanol was 609 days. Thet 1/2 values of (Z)7-, (E)7-, and (Z)9-tetradecen-1-ol acetates were 154, 168, and 199 days, respectively, whereas those of five other tested 14-carbon acetates ranged from 310 to 350 days. The dependence oft 1/2 values on chain length and double-bond position is consistent with the hypothesis that molecular size is an important variable affectingt 1/2 values. Also, in accordance with the hypothesis, when aZ-alkenyl compound has a much shortert 1/2 than the corresponding saturated compound, thet 1/2 values of theZ compound and itsE isomer may be quite different. Thus, (E)-9-tetradecen-l-ol acetate had at 1/2 of 331 days. Thet 1/2 of disparlure was 180 days. The effect of thecis-7,8 epoxide group is apparently similar to that of the olefin group in lowering thet 1/2 below the value that would be expected solely on the basis of chain length.
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    Relative efficiency of sticky and cylindrical electrical grid traps in capturingSpodoptera exigua (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 733-740 
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    ISSN: 1573-1561
    Keywords: Beet armyworm ; Spodoptera exigua ; Lepidoptera ; Noctuidae ; pheromone trap ; trap efficiency ; Pherocon 1C trap ; population monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative efficiency of sticky traps and cylindrical electrical grid traps for capturing male beet armyworm moths,Spodoptera exigua (Hubner), was studied using night vision equipment. The sticky and grid traps captured 34 and 40%, respectively, of the insects which oriented to them. The grid trap's capacity was limited only by the size of the receptacle (3.8-liter bucket) at the base of the funnel. The maximum capacity of the sticky trap was ca. 110 insects, and the efficiency of this trap declined rapidly when captures exceeded 50% capacity. This decline was caused by the physical blockage of the sticky surface by the captured insects. There was no evidence to indicate that captured males had any repelling effect on other males subsequently attracted to the sticky trap.
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    Comparative distribution and persistence of disparlure in woodland air after aerial application of three controlled-release formulations (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 867-880 
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    ISSN: 1573-1561
    Keywords: Disparlure ; gypsy moth ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; controlled release ; laminated dispensers ; hollow fibers ; microcapsules ; atmospheric permeation ; mating disruption ; air concentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Disparlure, the gypsy moth sex pheromone, was aerially applied to three plots, using a different controlled-release formulation-gelatin microcapsules, flakes of plastic laminates, or hollow fibers-in each plot. Disparlure concentrations in air were measured intermittently over a 34-day period after application. Measurements were made at four heights above ground: 0.3,2,5, and 10m. Wind speed and air temperature were measured concurrently. Disparlure was emitted from the microcapsules and fibers at very high levels for the first day or two; thereafter, all three formulations produced gradually decreasing aerial concentrations. After 32–34 days, concentrations in all plots ranged from 1.5% to 15.5% of those on the first day, although most of the pheromone remained in the formulations. Results suggest that the effective life of all three formulations for mating disruption by atmospheric permeation would be near 30 days under the test conditions, with fibers likely to have the shortest life. Concentrations varied diurnally throughout the experiment, with highest levels generally between 1400 and 2200 hr each day. Concentrations were highest at the 10-m height with microcapsules and at the 0.3- and 10-m heights with flakes, but were similar at all heights with fibers. Biological implications of the results are presented.
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    URL: http://dx.doi.org/10.1007/BF00992384
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    Alleviation of α-tomatine-induced toxicity to the parasitoid,Hyposoter exiguae, by phytosterols in the diet of the host,Heliothis zea (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 927-946 
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    ISSN: 1573-1561
    Keywords: Host-plant resistance ; α-tomatine ; sterols ; sterol esters ; Lycopersicon esculentum ; Heliothis zea ; Lepidoptera ; Noctuidae ; parasitoids ; Hyposoter exiguae ; Hymenoptera ; Ichneumonidae ; secondary compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The solitary, endoparasitic ichneumonid,Hyposoter exiguae (Viereck) is a parasitoid of the tomato fruitworm,Heliothis zea (Boddie). However, the parasitoid is deleteriously affected by the tomato glycoalkaloid, α-tomatine, ingested from hosts (H. zea) fed on artificial diets or semipurified extracts of tomato plants that contained α-tomatine. α-Tomatine causes prolonged larval development; disruption or prevention of pupal eclosion; deformation of antennal, abdominal, and genital structures; and reduction in adult weight and longevity of the parasitoid. These toxic effects are exacerbated when the dietary dose of α-tomatine is increased from 12 μmol to 20 μmol/g dry wt of diet. However, the toxicity of α-tomatine is attenuated in parasitoids reared from hosts fed on artificial diets that contain equimolar or supramolar amounts of 3β-OH-sterols admixed with α-tomatine. Further, the toxicity of extracts from the foliage of different cultivars of tomatoes toH. exiguae is contingent upon the composite levels of α-tomatine and total phytosterol (free and esterified). Cultivars with a low total sterol-tomatine ratio are more toxic toH. exiguae. The possible mode of action of α-tomatine toxicity toH. exiguae by disruption of sterol metabolism is discussed.
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    Identification of a sex pheromone ofHeliothis subflexa (GN.) (Lepidoptera: Noctuidae) and field trapping studies using different blends of components (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1011-1022 
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    ISSN: 1573-1561
    Keywords: Sex pheromone ; Heliothis subflexa ; Lepidoptera ; Noctuidae ; reproductive behavior ; field trapping ; capillary chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Eight compounds were isolated from the sex pheromone gland ofHeliothis subflexa (Gn.) and identified as hexadecanal, (Z)-9-hexadecenal, (Z)-11-hexadecenal, (Z)-7-hexadecen-1-ol acetate, (Z)-9-hexadecen-1-ol acetate, (Z)-11-hexadecen-1-ol acetate, (Z)-9-hexadecen-1-ol, and (Z)-11-hexadecen-1-ol. Although the whole blend was found to be an effective male attractant, the deletion of alcohols from the blend increased trap captures considerably. Further, although the binary mixture of (Z)-9-hexadecenal and (Z)-11-hexadecenal caught some maleH. subflexa, significant increases in captures were noted when the three acetate components were included in the blend.
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    Analysis of field-weathered lures containing (E)-10-dodecen-1-yl acetate, a sex attractant for the pea moth,Cydia nigricana (F.) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1049-1056 
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    ISSN: 1573-1561
    Keywords: Pea moth ; Cydia nigricana (F.) ; Lepidoptera ; Olethreutidae ; sex attractant ; (E)-10-dodecen-1-yl acetate ; lure ; formulation ; monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Natural rubber stoppers treated with 1, 3, or 10 mg of (E)-10-dodecen-1-yl acetate, an attractant for male pea moth,Cydia nigricana (F.), were exposed in the field during the summer of 1978 and samples analyzed at intervals. The results for all three doses fitted well to first-order loss curves with half-lives of 63.5, 64.7, and 67.3 days, respectively. Thus, lures with an initial dose of 3 mg of the attractant retained approximately 1 mg after 3 months of field exposure; they should therefore maintain a constant level of attractiveness throughout this period because previous field studies showed that moths were equally responsive to fresh lures containing between 1 and 10 mg of the attractant. There was no loss of attractant during the preparation of lures or after 4 months at −15 ° C and only 13% was lost during 3 months of storage at room temperature.
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    Baldcypress allelochemics and the inhibition of silkworm enteric microorganisms Some Ecological Considerations (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 103-114 
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    ISSN: 1573-1561
    Keywords: Baldcypress ; Taxodium distichum ; 2-furaldehyde ; 2-furoic acid ; Bombyx mori ; Lepidoptera ; Bombycidae ; enteric microbial inhibition ; Bacillus ; Micrococcus ; Arthrobacter ; Mucor ; Curvularia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Exposure ofBombyx mori larvae to 2-furaldehyde, a major volatile component of baldcypress heartwood,Taxodium distichum, resulted in in vivo inhibition of enteric microorganisms at concentrations (1–47 ppm) that were released naturally from heartwood. The 7 bacterial and 2 fungal enteric isolates were also inhibited in vitro at the same concentrations. It is suggested that inhibition of leaf surface micro-organisms or in vivo inhibition of silkworm enteric microflora, as a result of indirect or direct action of 2-furaldehyde, exacerbates the growth-inhibitory effects of this compound on larvae by reducing the microbial nutritional contribution. The ecological significance of insect enteric microbial inhibition by plant allelochemics is discussed.
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    On the persistence of disparlure in the human body (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 313-317 
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    ISSN: 1573-1561
    Keywords: Disparlure ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; pheromone persistence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Response of male gypsy moths from a sparse infestation in Italy to an observer apparently contaminated with disparlure at least two years previously is reported. Other examples of pheromone contamination of people or things are also recorded.
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    Comparing the effectiveness of sexual communication disruption in the oriental fruit moth (Grapholitha molesta) using different combinations and dosages of its pheromone blend (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 501-508 
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    ISSN: 1573-1561
    Keywords: Disruption of communication ; Oriental fruit moth ; Grapholitha molesta ; Lepidoptera ; Tortricidae ; sex pheromone ; sex attractant ; (Z)-8-dodecenyl acetate ; (E)-8-dodecenyl acetate ; (Z)-8-dodecen-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The relative efficacy of disruptant blends comprised of different combinations of the Oriental fruit moth's pheromone components was determined in field tests. Disruption was evaluated by comparing male moth catch at synthetic and female-baited traps in disruptant and non-treatment areas. Three atmospheric dosages of a 8-dodecenyl acetate (93.5%Z∶6.5%E) blend, representing two successive 10-fold decreases in concentration (2.5 × 10−2 g/hectare/day to 2.5 × 10−4 g/hectare/day) were tested alone and in combination with an additional percentage of (Z)-8-dodecen-1-ol. Male moth orientation to traps was eliminated in plots exposed to the two highest binary acetate dosages. However, significantly more males were captured in synthetic-baited traps in the lowest acetate-alone treatment, indicating a diminution of disruption efficiency. In contrast, inclusion of (Z)-8-dodecen-1-ol in the disruptant blend effected essentially complete disruption of orientation at all concentrations tested. Mating success ofG. molesta pairs confined in small cages apparently was not affected by the presence of relatively high concentrations of the binary acetate and the acetate-alcohol blends. This suggests that habituation and/or adaptation of male response, at least for comparatively “close-range” behaviors, did not occur.
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    Seven-week persistence of an oviposition-deterrent pheromone (1981)
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    Journal of chemical ecology 7 (1981), S. 583-588 
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    ISSN: 1573-1561
    Keywords: Oviposition ; deterrent pheromone ; Pieris brassicae ; Lepidoptera ; Pieridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Cabbage leaves sprayed with a water solution of the oviposition-deterrent pheromone ofPieris brassicae remain deterrent to ovipositing females for at least 14 days. Under laboratory conditions, the pheromone, when dried on a glass surface, retains activity for a period of at least 7 weeks. After 7 days under vacuum conditions, some pheromone is still present, indicating a low volatility and / or high stability of (an active fraction of) the pheromone. After 125 eggs are slowly rinsed with 300 ml water, they still release detectable quantities of the pheromone.
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    A four-component pheromone blend for optimum attraction of redbacked cutworm males,Euxoa ochrogaster (Guenée) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 615-625 
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    ISSN: 1573-1561
    Keywords: Redbacked cutworm ; Euxoa ochrogaster ; Lepidoptera ; Noctuidae ; sex pheromone blend ; sex attractant ; parapheromone ; Z-5-decenyl acetate ; Z-5-undecenyl acetate ; Z-5-dodecenyl acetate ; Z-7-dodecenyl acetate ; Z-9-dodecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Four acetates,Z-5-decenyl acetate,Z-5-,Z-7-, andZ-9-dodecenyl acetates, in microgram ratios of 1∶200∶2∶1 or 1∶200∶6∶2 were excellent, specific sex pheromone blends for capturing male redbacked cutworm moths in cone traps. Blends in ratios of 1∶200∶2∶1 and 2∶200∶2∶1 at 1000 μg/ rubber septum dispenser remained highly effective for 6 weeks under field conditions. The essential minor components,Z-5-decenyl,Z-7-, andZ-9-dodecenyl acetates, became inhibitory at concentrations of about 10% in the blends, and this may be an important general phenomenon in lepidopteran pheromones. Blends involving a parapheromone,Z-5-undecenyl acetate, withZ-5-,Z-7-, andZ-9-dodecenyl acetate, in microgram ratios of 8∶200∶2∶1 or 20∶200∶6∶2 were also excellent specific attractants for this species. TheZ-8-dodecenyl acetate had no obvious effect on the attraction of the redbacked cutworm males.
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    Kairomones and their use for management of entomophagous insects: X. Laboratory studies on manipulation of host-finding behavior ofTrichogramma pretiosum riley1 with a kairomone extracted fromHeliothis zea (boddie) moth scales (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 635-648 
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    ISSN: 1573-1561
    Keywords: Kairomone ; parasitoids ; biological control ; Trichogramma pretiosum ; Hymenoptera ; Trichogramma tidae ; Heliothis zea ; Lepidoptera ; Noeturidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The behavioral response ofTrichogramma pretiosum Riley females to the kairomone found inHeliothis zea (Boddie) moth scales is examined.
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    Sex pheromone of the saltmarsh caterpillar moth,Estigmene acrea (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 655-668 
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    ISSN: 1573-1561
    Keywords: Saltmarsh caterpillar moth ; Estigmene acrea ; (Z,Z)-9,12-octadecadienal ; (Z,Z,Z)-9,12,15-octadecatrienal ; (Z,Z)-3,6-cis-9,10-epoxy-heneicosadiene ; fall webworm moth ; Hyphantria cunea ; insect sex pheromone ; cis-9,10-epoxyheneicosane ; trans-9,10-epoxyheneicosane ; linolealde-hyde ; linolenaldehyde ; Lepidoptera ; Arctiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three compounds have been identified as components of the sex pheromone emitted by females of the saltmarsh caterpillar moth,Estigmene acrea (Drury). These are (Z,Z)-9,12-octadecadienal (I), (Z,Z,Z)-9,12,15-octadecatrienal (II), and (Z,Z)-3,6-cis-9,10-epoxyheneicosadiene (III). In female tip extract they were found in a ratio of 1∶6∶25, respectively, and in trapped female effluvia the ratio was 1∶6∶27, respectively. Combinations of III with either I or II elicited sustained upwind flight in a wind tunnel, but none of these compounds by themselves did so. There is evidence that the antennal acceptor site for III is chiral.
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    Effect of day length and light intensity on 2-tridecanone levels and resistance inLycopersicon hirsutum f.glabratum toManduca sexta (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 707-716 
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    ISSN: 1573-1561
    Keywords: Manduca sexta (L.) ; Lepidoptera ; Sphingidae ; Lycopersicon hirsutum f.glabratum ; day length ; light intensity ; 2-tridecanone ; glandular trichome ; resistance ; allelochemic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract First instarManduca sexta (L.) larvae confined on foliage fromLycopersicon hirsutum f.glabratum (accession PI 134417) plants grown under a long-day regime exhibited greater mortality than larvae on foliage from plants grown under a short-day regime. 2-Tridecanone, a toxin important in the insect resistance of PI 134417, was significantly more abundant in the foliage of plants grown under the long-than the short-day regimes. Light intensity influenced neither 2-tridecanone levels nor the expression of resistance. The density of glandular trichomes, which secrete 2-tridecanone, was influenced by an interaction between day length and light intensity.
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    Growth inhibitors in tomato (Lycopersicon) to tomato fruitworm (Heliothis zea) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 753-758 
    Details
    ISSN: 1573-1561
    Keywords: Lycopersicon esculentum ; Lycopersicon hirsutum f. glabratum Heliothis zea ; Lepidoptera ; Noctuidae ; α-tomatine ; rutin ; chlorogenic acid ; caffeyl aldaric acid ; allelochemics ; tomato ; tomato fruitworm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Several compounds that retard the larval growth of the tomato fruitworm,Heliothis zea (Boddie) have been isolated and identified from tomato leaves,Lycopersicon esculentum Mill. The major allelochemics are α-tomatine (I), chlorogenic acid (II), rutin (III), and a new caffeyl derivative of an aldaric acid (IV). The isolation, analyses, and toxicity of these compounds toH. zea are presented.
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    Biological amplification for increasing electroantennogram discrimination between two female sex pheromones ofSpodoptera littoralis (Lepidoptera: Noctuidae) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 791-798 
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    ISSN: 1573-1561
    Keywords: Spodoptera littoralis ; Lepidoptera ; Noctuidae ; electroantennogram ; biological amplification ; pheromones ; Boarmia selenaria ; Geometridae ; Matsucoccus josephi ; Homoptera ; Coccoidea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract MaleSpodoptera littoralis stimulated by a mixture ofS. littoralis female sex pheromone with a positional isomer and by the pheromone alone, gave greatly amplified electroantennograms (EAGs) when the antennae were connected in series. The technique is described and examples are given of its use in screening extracts for EAG activity. Dose-response curves obtained with this technique are presented and discussed. Through biological amplification, EAG discrimination between the pheromones was increased.
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    Sex pheromone components isolated from china corn borer,Ostrinia furnacalis guenée (lepidoptera: Pyralidae), (E)- and (Z)-12-tetradecenyl acetates (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 841-851 
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    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pyralidae ; Ostrinia furnacalis ; sex pheromone ; corn borer ; (E)-12-tetradecenyl acetate ; (Z)-12-tetradecenyl acetate ; tetradecyl acetate ; gas chromatography-mass spectrometry ; selected ion monitoring technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The sex pheromone components from the corn borer spreading widely in China,Ostrinia furnacalis Guenée, have been identified as (E)-and (Z)-12-tetradecenyl acetates (E andZ12–14∶ Ac). The ratio ofE isomer toZ isomer was 53 ∶ 47. Traps containing 1 × 10−7−1 × 10−5 g of these compounds captured more males than did live females or their tip extract (3–6 female equivalents). Tetradecyl acetate (14 ∶ Ac) was also identified in the tip extract. Its quantity was about 1.8 times the sum of the other two isomers. However, including this compound in its natural ratio in pheromone traps resulted in a decrease in trap catches (P〈 0.05).
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    Kairomones and their use for management of entomophagous insects (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 909-917 
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    ISSN: 1573-1561
    Keywords: Trichogramma pretiosum ; Hymenoptera ; Trichogrammatidae ; biological control ; kairomone ; Heliothis zea ; Lepidoptera ; Noctuidae ; host density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Trichogramma pretiosum Riley females exhibit success-motivated searching after oviposition. The stimulatory effect of contact with host eggs makes host-egg density an important factor in determining the appropriate strategy for behavioral manipulation, using kairomones, that simulate host seeking, in biological control programs. Host eggs can be used, in conjunction with kairomones or by themselves, to improve the performance of these important beneficial insects.
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    Kairomones and their use for management of entomophagous insects (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1057-1061 
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    ISSN: 1573-1561
    Keywords: Heliothis zea ; Lepidoptera ; Noctuidae ; Microplitis croceipes ; Hymenoptera ; Braconidae parasitoids ; kairomones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In laboratory experiments conducted to compare the kairomonal activity forMicroplitis croceipes (Cresson) of frass fromHeliothis zea (Boddie) larvae fed on different host plants,M. croceipes females responded to extracts of frass from larvae reared on cotton or soybeans but not on corn. The lack of response to frass from larvae reared on corn was shown to be a result of a lack of some appropriate chemical(s) in the corn.
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    Sex pheromone of the tomato looper,Plusia chalcites (ESP) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1081-1088 
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    ISSN: 1573-1561
    Keywords: Plusia chalcites ; Lepidoptera ; Noctuidae ; tomato looper ; sex pheromone identification ; glass WCOT capillary chromatography ; gas chromatography-mass spectrometry ; Z-7-dodencenyl acetate ; Z-9-tetra-decenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Z-7-Dodecenyl acetate (A) andZ-9-tetradecenyl acetate (B), in a 5∶1 ratio, have been identified as sex pheromone components of femalePlusia chalcites. In addition, small amounts of dodecyl acetate, tetradecyl acetate, hexadecyl acetate, andZ-9-dodecenyl acetate have been found in the abdominal tip extracts. This is the first known Plusiinae species which utilizes a two-component primary sex pheromone system. The most effective lure found in field experiments was a mixture of 1 mg A and 0.2 mg B absorbed on rubber septa.
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    Larval osmeterial secretions of the swallowtails (Papilio) (1981)
    Springer
    Journal of chemical ecology 7 (1981), S. 1089-1113 
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    ISSN: 1573-1561
    Keywords: Osmeterial secretion ; Papilio larvae ; Lepidoptera ; monoterpene ; sesquiterpene ; aliphatic acid ; ester
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The larval osmeterial secretions of sixPapilio species examined displayed a remarkable qualitative change at the fourth larval ecdysis. The secretions of 4th (penultimate) instar larvae ofP. machaon, P. memnon, P. helenus, P. bianor, andP. maackii principally comprised mono- and/or sesquiterpenoids. The compounds identified from these species included α-pinene, sabinene, β-myrcene, limonene, β-phellandrene, (Z)-β-ocimene, (E)-β-ocimene, β-elemene, β-caryophyllene, (E)-β-farnesene, β-selinene, (E,E)-α-farnesene, germacrene-A, germacrene-B, caryophyllene oxide, methyl 3-hydroxy-n-butyrate, and acetic acid. In contrast, the secretion of 4th larval instar ofP. xuthus, although containing similar terpenic compounds, was accompanied by large amounts of aliphatic acids and their esters: isobutyric acid, 2-methylbutyric acid, methyl isobutyrate and methyl 2-methylbutyrate. On the other hand, the osmeterial secretions of 5th (last) instar larvae varied little in quality among the six species, and the identified compounds consisted of isobutyric acid, 2-methylbutyric acid, methyl isobutyrate, ethyl isobutyrate, methyl 2-methylbutyrate, ethyl 2-methylbutyrate, and isovaleric acid, the last of which was specific toP. bianor andP. maackii. The chemical alteration of osmeterial exudate synchronized at the final larval ecdysis with the larval morphological change (particularly in body coloration) that appears to be of defensive significance against predators.
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    Apparent long-term bodily contamination by disparlure, the gypsy moth (Lymantria dispar) Attractant (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 33-37 
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    ISSN: 1573-1561
    Keywords: Disparlure ; Lymantria dispar ; Lepidoptera ; Lymantriidae ; gypsy moth ; pheromone persistence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Positive attraction of male gypsy moths to the body of the author, now four years after his last known direct contact with disparlure, the synthetic pheromone, is documented. A designed test showed that moths responded to him in highly significantly greater numbers than to others who had had less or no previous exposure to the insect and / or disparlure. The data present persuasive evidence of bodily contamination with disparlure and suggest that the material is very persistent.
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    Triunsaturated hydrocarbons, sex pheromone components ofCaenurgina erechtea (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 1413-1423 
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    ISSN: 1573-1561
    Keywords: Behavior ; Caenurgina erechtea (Cramer) ; forage looper ; Lepidoptera ; Noctuidae ; sex pheromone ; (Z,Z,Z)-3,6,9-eicosatriene ; (Z,Z,Z)-3,6,9-heneicosatriene ; flight tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z,Z,Z)-3,6,9-Eicosatriene and (Z,Z,Z)-3,6,9-heneicosatriene have been identified as components of the sex pheromone of the noctuid,Caenurgina erechtea (Cramer), the forage looper. Structural assignments were made on the basis of spectroscopic and chromatographic data and were confirmed by comparison with synthetic material. Flight tunnel behavioral studies demonstrated that either component, when tested individually, would elicit wing fanning responses in males; however, mixtures of the two components increased this response and were essential for initiation of upwind flight and landing. In field experiments, traps baited with either component alone captured few or no adult forage looper males while those baited with both components captured several target males.
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    Sex pheromone components of the European goat moth,Cossus cossus (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 191-200 
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    ISSN: 1573-1561
    Keywords: Sex pheromone ; Cossus cossus ; Lepidoptera ; Cossidae ; (Z)-5-dodecenyl acetate ; (Z)-5-dodecenol ; (Z)-5-tetradecenyl acetate ; (Z)-3-decenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Female tip washings of the European goat moth,Cossus cossus L., were found to contain the following compounds that are structurally similar to known pheromone components of Lepidoptera (%): decyl acetate (1.5), (Z)-5-dodecenol (1), (Z)-5-dodecenyl acetate (66), dodecyl acetate (12), (Z)-5-tetradecenyl acetate (14), tetradecyl acetate (4), and hexadecyl acetate (1.5). The washes contained an average of 125 ng of (Z)-5-dodecenyl acetate per female equivalent. EAG responses to nanogram amounts of the identified products indicated that (Z)-5-dodecenyl acetate was the most potent olfactory stimulant followed by (Z)-5-tetradecenyl acetate and (Z)-5-dodecenol, whereas the saturated acetates caused only weak depolarization. A strong EAG response was also recorded for (Z)-3-decenyl acetate, which was not detected in the female tip washings. Field results showed that (Z)-5-dodecenyl acetate was essential for the attraction of the males and that (Z)-3-decenyl acetate improved the attractiveness of (Z)-5-dodecenyl acetate alone or in combination with (Z)-5-tetradecenyl acetate.
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    Synthesis of (Z)-13-hexadecen-11-yn-1-yl acetate Major component of sex pheromone of the processionary moth (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 219-223 
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    ISSN: 1573-1561
    Keywords: Synthesis ; sex pheromone ; processionary moth ; Thaume-topoea pityocampa ; Lepidoptera ; Notodontidae ; (Z)-13-hexadecen-11-yn-1-yl acetate ; acetylene ; carbocupration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The synthesis of the major component of the sex pheromone of the processionary mothThaumetopoea pityocampa is described. The synthesis uses the carbocupration of acetylene, followed by the coupling with the appropriate 1-iodoalkyne. This synthetic pheromone was shown to be of 98.8%Z purity.
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    Sex pheromone components ofEuxoa drewseni Chemical identification, electrophysiological evaluation, and field attractancy tests (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 335-346 
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    ISSN: 1573-1561
    Keywords: Euxoa drewseni ; Chorizagrotis thanatologia ; Lepidoptera ; Noctuidae ; sex pheromone ; attractant inhibitor ; parapheromone ; (Z)-5-dodecenyl acetate ; (Z)-7-dodecenyl acetate ; (Z)-7-tetradecenyl acetate ; (Z)-7-pentadecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Eleven compounds structurally similar to known lepidopterous pheromone components were identified in the extract from 18 calling female moths ofEuxoa drewseni (Staudinger). The identifications were done by gas chromatography-mass spectrometry and high-resolution gas chromatography with flame ionization and electroantennographic detectors simultaneously. Detector antennae were from five species of moths. In the field, male moths were specifically attracted to a three-component blend of dodecyl, (Z)-5-dodecenyl, and (Z)-7-tetradecenyl acetates in a ratio of 2∶6∶1. This blend at 1000 μg/rubber septum dispenser is recommended as a trap bait for monitoring purposes. Low concentrations of (Z)-7-dodecenyl acetate or (Z)-7-tetradecenol inhibited the attraction of moths to the three-component blend. (Z)-7-Pentadecenyl acetate functioned as a parapheromone in place of (Z)-7-tetradecenyl acetate in the pheromone blend, and they appear to react via the same antennal receptor.
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    Cardenolide sequestration by the dogbane tiger moth (Cycnia tenera; Arctiidae) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 521-532 
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    ISSN: 1573-1561
    Keywords: Cycnia tenera ; Arctiidae ; Lepidoptera ; Asclepias ; milkweeds ; cardenolides ; cardiac glycosides ; allelochemics ; plant-insect interactions ; plant secondary chemistry ; chemical ecology ; chemical defense ; kin selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Cycnia tenera adults, reared as larvae onAsclepias humistrata, had 10 times higher cardenolide concentrations, and contained 15 times more total cardenolide, than did moths reared onA. tuberosa. Thin-layer chromatography confirmed that each individual cardenolide visualized in the adult moths reared on the former host plant corresponds to one present in the plant, thus demonstrating that the insects' cardenolides are indeed derived from the larval food. Adult weights were significantly greater when the larvae had been fed upon the higher cardenolide plant species,A. humistrata. Similar results for other milkweed-feeding insects have been interpreted by some authors as evidence against a metabolic cost of handling cardenolides. However, such interpretations confound cardenolide differences among milkweed species with other differences in plant primary and secondary chemistry that affect insect growth and development. While the cooccurrence inC. tenera of other noxious chemicals (e.g., alkaloids) is not precluded, cardenolides sequestered from larval host plants have probably contributed to the evolution of visual and auditory aposematism in this species. As the eggs are laid in large clutches and larvae are gregarious, such aposematism may have evolved via kin selection.
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    Identification of a sex pheromone produced by female velvetbean caterpillar moth (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 645-656 
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    ISSN: 1573-1561
    Keywords: Sex pheromone ; 3,6,9-heneicosatriene ; 3,6,9-eicosatriene ; velvetbean caterpillar ; Anticarsia gemmatalis Hübner ; Lepidoptera ; Noctuidae ; attractant ; hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A sex pheromone produced by female velvetbean caterpillar moths,Anticarsia gemmatalis Hübner, that attracts conspecific males was isolated and identified as a blend of (Z,Z,Z)-3,6,9-eicosatriene and (Z,Z,Z)-3,6,9-heneicosatriene in a ratio of ca. 5∶3, respectively, when combined. The synthesized compounds elicited responses by velvetbean caterpillar moth males equivalent to those elicited by females in both laboratory wind tunnel bioassays and field trapping experiments.
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    Effect of soybean phytoalexins on the herbivorous insects mexican bean beetle and soybean looper (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 657-672 
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    ISSN: 1573-1561
    Keywords: Pseudoplusia includens ; soybean looper ; Lepidoptera ; Noctuidae ; Epilachna varivestis ; Mexican bean beetle ; Coleoptera ; Coccinellidae ; feeding preferences ; nutrition ; food utilization ; host-plant resistance ; induced resistance ; glyceollin ; isoflavonoids ; soybean ; phytoalexins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Effects of soybean phytoalexins on the feeding of the soybean looper and Mexican bean beetle were investigated to test the hypothesis that phytoalexins might be a defense mechanism of plants against insects as well as against pathogens. Short-term behavioral responses to the phytoalexins were analyzed using dual-choice tests with phytoalexin-rich and phytoalexin-poor (control) tissues. Phytoalexin production was elicited with ultraviolet radiation. Results from the dual-choice tests indicated that 6th instar soybean looper larvae fed equally on the control and phytoalexin-rich tissues. Feeding by adult and 4th instar Mexican bean beetles, however, was strongly deterred by the phytoalexins as evidenced by “single-bite” mandible scars on the phytoalexin-rich cotyledon discs. Nutritional effects of the isoflavonoid phytoalexin glyceollin on early instar soybean looper larvae were tested by incorporating the phytoalexin into an artificial medium at a level of 1% dry weight (0.15% fresh weight). The larvae were reared for 7 days from emergence on diets of control and glyceollin-containing media. Although survival on the glyceollin diets was initially less than on the control diets, under the experimental conditions glyceollin had no significant effect on the growth, development, or subsequent survival of the larvae. Efficiency of food utilization (ECI) was reduced, indicating that the phytoalexins may be a mild digestibility-reducing factor for the loopers. Implications of the results for host-plant resistance are discussed.
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    Identification of sex pheromone components of the female driedfruit moth,Vitula edmandsae serratilineella, and a blend for attraction of male moths (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 785-801 
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    ISSN: 1573-1561
    Keywords: Driedfruit moth ; Vitula edmandsae serratilineella ; Lepidoptera ; Pyralidae ; Phycitinae ; sex pheromone ; attractant inhibitor ; (Z)-9,(E)-12-tetradecadienol ; (Z)-9,(E)-12-tetradecadienal ; alcohol ; aldehyde
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sixteen pheromone-like compounds were identified in abdomen tip washes and excised abdomen tip extracts of calling females of driedfruit moth,Vitula edmandsae serratilineella Ragonot. Identifications were by high-resolution gas chromatography-mass spectrometry, and gas chromatography with flame ionization and electroantennographic detectors. Male moths were attracted to a blend of (Z)-9,(E)-12-tetradecadienol and (Z)-9,(E)-12-tetradecadienal in a ratio of 100∶1 at 500 μg/rubber septum dispenser, which is recommended for monitoring purposes. Low concentrations of (Z)-9-tetradecenol (0.5%) and (Z)-9-tetradecenal (0.1%) may be beneficial for the attraction of males, but 1–2% of (E)-9,(E)-12- or (Z)-9,(Z)-12-tetradecadienol, or (Z)-9,(E)-12-tetradecadienyl acetate inhibited their attraction. Gravid female moths were attracted to traps that captured large numbers of males. Females may be attracted to male hairpencil or forewing gland secretions emitted near the traps or that accumulate in the traps.
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    Stereoselective synthesis of (Z)-13-hexadecen-11-yn-1-yl acetate, the major component of the sex pheromone of the pine processionary moth (Thaumetopoea pityocampa) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 863-867 
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    ISSN: 1573-1561
    Keywords: (Z)-13-Hexadecen-11-yn-1-yl acetate ; pine processionary moth ; Thaumetopoeapityocampa ; Thaumetopoea wilkinsoni ; Lepidoptera ; Notodontidae ; sex pheromone ; synthesis ; biological activity ; ester
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A short and stereoselective synthesis of (Z)-13-hexadecen-1 1-yn-1-yl acetate is described. The main feature is a low-temperature Wittig reaction of a triphenylpropylphosphonium bromide with a long-chain alkylated propargyl aldehyde.
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    Field attractiveness of (E)- and (Z)-11-tetradecenal pheromone blends to male spruce budworm moths,Choristoneura fumiferana (Clemens) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 1327-1332 
    Details
    ISSN: 1573-1561
    Keywords: Spruce budworm moths ; Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; sex pheromone ; attractants ; isomeric blends
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract E∶Z blends of (E)-11-tetradecenal were field tested (three experiments) for their attractiveness to male spruce budworm,Choristoneura fumiferana (Clemens), moths in northern Maine. Blends of 92.5–99%E isomer caught the most moths (three experiments); blend 95%E had the highest cumulative catch throughout two experiments. Rates of catch per hour for the four most attractive blends (92.5–99%E) showed highly variable responses among experiments; however, similarities were noted for rates of catch within the same experiment. For all experiments and observation hours, blend 95%E had the highest mean rate of catch.
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    Initial field trials with the synthetic sex pheromone of the processionary mothThaumetopoea pityocampa (Denis and Schiff.) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 85-93 
    Details
    ISSN: 1573-1561
    Keywords: Sex attractant ; sex pheromone ; processionary moth ; Thaumetopoea pityocampa ; Lepidoptera ; Notodontidae ; (Z)-13-hexadecen-11-ynyl acetate ; field test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Synthetic (Z)-13-hexadecen-11-ynyl acetate has been shown to be highly active in catchingThaumetopoea pityocampa (Denis and Schiff.) males in field trials carried out in different parts of Spain. A variety of formulations containing antioxidants or solid paraffin were tested. Formulations containing 3 and 10% ofE isomer showed a decrease of activity compared with those prepared with pureZ isomer. Dodecyl acetate, also found in the virgin female gland, did not show any synergistic effect when tested in a 9∶1 mixture with the synthetic pheromone. The product exhibited a remarkable persistence of activity under the field conditions even in the absence of stabilizer.
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    Spodoptera exigua: Capture of feral males in traps baited with blends of pheromone components (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 95-104 
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    ISSN: 1573-1561
    Keywords: Spodoptera exigua ; Lepidoptera ; Noctuidae ; pheromone ; rubber septa ; (Z,E)-9,12-tetradecen-1-ol acetate ; (Z)-9-tetradecen-1-ol ; beet armyworm ; trapping sticky trap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Field tests were conducted with various blends of acetates and alcohols previously identified as components of the sex pheromone for the beet armyworm (BAW),Spodoptera exigua (Hübner). The compounds were formulated on rubber septa and placed in sticky traps positioned in fields populated withAmaranthus sp., a favored host of this species. Moth captures were highest in traps baited with septa containing a blend of 0.1 mg (Z,E)-9,12-tetradecadien-1-ol acetate (Z9,E12–14∶Ac) and 0.01 mg(Z)-9-tetradecen-1-ol (Z9–14∶OH). Additionally, we confirmed that all combinations of acetates tested alone captured significantly fewer males than blends containing a 10∶1 ratio ofZ9,E12–14∶Ac andZ9–14∶OH. A 10∶1 formulation ofZ9,E12–14∶Ac andZ9–14∶OH in hollow fibers was also attractive to feral BAW males.
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    Field trapping of diamondback mothPlutella xylostella using an improved four-component sex attractant blend (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 113-118 
    Details
    ISSN: 1573-1561
    Keywords: Diamondback moth ; Plutella xylostella ; Crymodes devastator ; Lepidoptera ; Yponomeutidae ; Noctuidae ; sex attractant ; (Z)-11-hexadecenyl acetate ; (Z)-11-hexadecen-1-ol ; (Z)-11-hexadecenal ; (Z)-9-tetradecenyl acetate ; (Z)-9-tetradecen-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In addition to three known sex lure components [(Z)-11-hexadecenyl acetate, (Z)-11-hexadecenal, and (Z)-11-hexadecenol], (Z)-9-tetradecenyl acetate was field-proven as a trace coattractant for malePlutella xylostella, with an optimal content below 0.01% in blends. This potent four-component lure for diamondback males also attractedCrymodes devastator males, and in this respect is not different in its attractancy from virgin diamondback females. Replacement of (Z)-9-tetradecenyl acetate in the four component lure with (Z)-9-tetradecen-1-ol, at the level of 10% of the total lure mixture, did not alter its attractancy for diamondback males, but it did inhibit attraction ofCrymodes devastator. The status of biologically active components as possible sex pheromones or para-pheromones is discussed.
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    Chemical basis for host selection byHemileuca oliviae (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 1425-1437 
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    ISSN: 1573-1561
    Keywords: Range caterpillar ; Hemileuca oliviae ; Lepidoptera ; Saturniidae ; C4 plants ; C3 plants ; tannins ; grasses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract When provided a choice between grass species with C3 or C4 photosynthetic pathways, larvae of range caterpillar,Hemileuca oliviae Cockerell, selected C4 grasses. The basis for host selection was examined by conducting analyses of moisture, crude protein, total available carbohydrate, sucrose, glucose, astringency, condensed tannin, silica, and pubescence of 14 grass species, and correlating host plant chemical characteristics with host preference. Most of the variation in host preference was explained by tannin characteristics (astringency and condensed tannin); C3 grass species had significantly higher tannin levels than C4 species.
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    Identification of two components of the sex pheromone of the moth,Epiphyas postvittana (Lepidoptera, Tortricidae) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 503-512 
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    ISSN: 1573-1561
    Keywords: Epiphyas postvittana ; Lepidoptera ; Fortricidae ; lightbrown apple moth ; sex pheromone ; (E)-11-tetradecen-1-yl acetate ; (E,E)-9,11-tetradecadien-1-yl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two compounds, (E)-11-tetradecen-1-yl acetate and (E,E)-9,11-tetradecadien-1-yl acetate, have been identified in extracts of females of the lightbrown apple moth,Epiphyas postvittana (Walker). The two compounds are active as a coalitive pair and are present in extracts of females in a ratio of about 20∶1.
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    The role of iridoid glycosides in host-plant specificity of checkerspot butterflies (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 475-493 
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    ISSN: 1573-1561
    Keywords: Iridoid glycoside ; catalpol ; Scrophulariaceae ; Euphydryas ; checkerspot ; host-plant specificity ; Lepidoptera ; Nymphalidae ; coevolution ; insect-plant interaction ; chemical ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The potential role of iridoid glycosides as feeding stimulants forEuphydryas chalcedona larvae was examined in three laboratory experiments. The first experiment examined larval behavior in choice tests between an artificial diet with no additives (AD) and an artificial diet with the iridoid glycoside, catalpol, added (AD + I) in one group; and AD and AD plus a crude extract from which the iridoid glycoside catalpol was crystallized (AD + Ex) in the second group. The larvae were found more often on AD + I or AD + Ex. The second experiment quantified larval consumption of artificial diets when given a choice of AD or AD + I, and AD or AD + Ex, and showed that larvae ate significantly more AD + I or AD + Ex than AD. The third experiment compared growth and survival on six diets: AD; AD + I; artificial diet with dried, ground upScrophularia californica leaves (AD + S); artificial diet with dried, ground upPlantago lanceolata leaves (AD + P);S. californica leaves (S); andP. lanceolata leaves (P). Growth was best onS. californica leaves, and survival was highest onS. californica andP. lanceolata leaves. There were no differences in growth rate or survival between AD andAD + I. Thus, iridoid glycosides serve as feeding attractants and stimulants for larvae ofEuphydryas chalcedona and are suggested as the basis of radiation in butterflies of the genusEuphydryas.
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    Kairomones and their use for management of entomophagous insects (1983)
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    Journal of chemical ecology 9 (1983), S. 695-701 
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    ISSN: 1573-1561
    Keywords: Kairomone ; pheromone ; parasitoid ; Telenomus remus ; Hymenoptera ; Scelionidae ; Spodoptera frugiperda ; Lepidoptera ; Noctuidae ; fall armyworm ; (Z)-9-tetradecene-1-ol acetate ; (Z)-9-dodecene-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Telenomus remits Nixon is a parasitoid that attacks egg masses ofSpodoptera frugiperda (J.E. Smith). Increased host-seeking behavior was elicited fromT. remus females in Y-tubes, Petri dish, and greenhouse bioassays byS. frugiperda female abdominal tips as well as (Z)-9-tetradecene-1-ol acetate and (Z)-9-dodecene-1-ol acetate.
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    Dose-response relationship for two components of the sex pheromone of lightbrown apple moth,Epiphyas postvittana (Lepidoptera: Tortricidae) (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 715-725 
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    ISSN: 1573-1561
    Keywords: Epiphyas postvittana ; Lepidoptera ; Tortricidae ; sex pheromone ; dose-response surface ; isobole ; male response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bioassay studies with mixtures of the two components from the sex pheromone ofE. postvittana are used to construct a dose-response surface. The surface clearly shows the existence of a response maximum and that an optimum ratio of the two components applies over a wide range of concentrations. Probit transformations of the same data are used to construct an isobologram. The isobole has a sharp minimum at a ratio for the two components close to the ratio produced by the females.
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    Behavioral responses of maleHeliothis virescens in a sustained-flight tunnel to combinations of seven compounds identified from female sex pheromone glands (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 747-759 
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    ISSN: 1573-1561
    Keywords: Tobacco budworm ; Heliothis virescens ; Lepidoptera ; Noctuidae ; flight tunnel ; sex pheromone ; moth behavior ; rubber septa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Each of the seven compounds that have been identified from femaleHeliothis virescens sex pheromone glands was examined for its ability to elicit sexual responses from male moths in a flight tunnel. The two compounds initially described as pheromone components, (itZ)-11-hexadecenal and (itZ)-9-tetradecenal, were necessary for behavioral activity to occur. Of the remaining five compounds, hexadecanal was most consistent in elevating behavioral activity of males when it was added to treatments. Live, calling females elicited greater sexual activity from males than did the 7-compound mixture on rubber septa.
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    Experiments with a two-component sex attractant of the silver Y moth (Autographa gamma L.), and some evidence for the presence of both components in natural female sex pheromone (1983)
    Springer
    Journal of chemical ecology 9 (1983), S. 1317-1325 
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    ISSN: 1573-1561
    Keywords: Insect sex pheromone ; sex attractant ; Autographa gamma L. ; Lepidoptera ; Noctuidae ; silver Y moth ; (Z)-7-dodecen-1-yl acetate ; (Z)-7-dodecen-1-ol ; ester ; alcohol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z)-7-Dodecen-1-yl acetate and (Z)-7-dodecen-1-ol were synthesized and tested on males of the silver Y moth (Autographa gamma L.) for sex attractant activity. The key step of the synthesis was the isomerization of acetylenic alcohol (III) with potassium 3-amino-propylamide. In EAG tests with a series of dodecen-1-yl acetates and alcohols, the highest activity was elicited by these two compounds. In field tests using three different kinds of dispensers, highest catches were achieved with a mixture of (Z)-7-dodecen-1 -yl acetate and (Z)-7-dodecen-1 -ol which contained 1–5% of the alcohol. Some evidence was also found for the presence of both compounds in extracts of the abdominal tip of females. The quantities of these components in the extract was 1.0 ng/female for the acetate, and 1.1 ng/female for the alcohol.
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    Effects of small doses of colchicine on the components of the hypothalamo-neurohypophysial system of the rat (1981)
    Springer
    Cell & tissue research 220 (1981), S. 809-827 
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    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Neurophysins ; Intra-axonal transport ; Immunocytochemistry ; Colchicine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Small doses (3.5 μg and 7 μg) of colchicine injected intracisternally caused an interruption of transport of secretory material from the supraoptic and paraventricular nuclei of the hypothalamus to the neural lobe of the pituitary gland. Transport was assessed by direct measurement of the incorporation of [35S] cysteine into neurophysins, by radioimmunoassay of accumulated material in discrete areas of the system and by immunocytochemistry. The larger dose (7 μg) switched off transport completely during the first 24 h but the system began to recover within three to four days. Colchicine had little, if any, effect on synthesis; comparison of the relationships of the apparent amounts of immunoreactive neurophysins and immunoreactive hormones in the arrested product led to the conclusion that processing of the hormone precursors continues within the secretory granules which accumulated in the perikarya.
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    Coexistence of cholecystokinin and oxytocin-neurophysin in some magnocellular hypothalamo-hypophyseal neurons (1981)
    Springer
    Cell & tissue research 221 (1981), S. 227-231 
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    ISSN: 1432-0878
    Keywords: Magnocellular neurosecretory system ; Cholecystokinin ; Oxytocin-neurophysin neurons ; Immunocytochemistry ; Neuropeptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The existence of cholecystokinin in the posterior hypophysis and its hypothalamic origin have been unequivocally demonstrated. Immunocytochemical evidence is presented for the coexistence of gastrin-cholecystokinin and oxytocin-neurophysin I immunoreactivities in some magnocellular neurons of the supraoptic and paraventricular nuclei both in rat and bovine hypothalamus.
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    Cross-reactivity between human and fish pituitary hormones as demonstrated by immunocytochemistry (1981)
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    Cell & tissue research 221 (1981), S. 257-267 
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    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Human pituitary hormone antisera ; Platyfish ; Pituitary gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this communication we describe the immunocytochemical cross-reactivity between antisera to various human pituitary hormones and specific hormone producing cell types in the pituitary gland of sexually mature male platyfish (Xiphophorus maculatus). Antisera to human pituitary hormones cross-reacted either with cells known to produce corresponding hormones (or hormone subunits) in the platyfish (e.g., ACTH, prolactin, TSH β, LH α, FSH α, TSH α) or with no pituitary cells at all (e.g., LH β, FSH β). The one exception was antiserum to human growth hormone which cross-reacted with MSH and ACTH producing cells. The platyfish pituitary is proposed as a test system for immunocytochemically screening antisera for purity and specificity in order to determine their applicability in particular studies.
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    Immunocytochemical study of luteinizing hormone in the pituitary of the sea lamprey, Petromyzon marinus L., during its upstream migration (1983)
    Springer
    Cell & tissue research 230 (1983), S. 225-228 
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    ISSN: 1432-0878
    Keywords: Pituitary ; Adenohypophysis ; Luteinizing hormone (LH) ; Immunocytochemistry ; Lamprey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A mammalian-like immunoreactive luteinizing hormone (LH) has been localized in the cytoplasm of cells in the meso-adenohypophysis of the sea lamprey, Petromyzon marinus L. Specific staining for LH was absent in other regions of the lamprey pituitary.
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    Immunocytochemical localization of somatostatin and vasotocin in the brain of the Pacific hagfish, Eptatretus stouti (1983)
    Springer
    Cell & tissue research 229 (1983), S. 541-550 
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    ISSN: 1432-0878
    Keywords: Hypothalamus ; Hagfish ; Somatostatin neurons ; Vasotocin neurons ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The brain of the Pacific hagfish, Eptatretus stouti, was studied immunocytochemically using antisera against somatostatin (SRIH), arginine vasopressin (AVP), and adrenocorticotropic hormone (ACTH). SRIH-immunoreactive perikarya were distributed bilaterally in the postoptic nucleus and in the hypothalamic nucleus. Although several short, stained fibers were observed in the vicinity of the perikarya, SRIH-immunoreactivity was not found in the neurohypophysis, nor in other parts of the brain. On the other hand, presumed arginine vasotocin (AVT) perikarya were distributed in an arc-shaped region extending from the posterior part of the preoptic nucleus to the anterior-most end of the hypothalamic nucleus and projected their fibers to the neurohypophysis. Most presumptive AVT perikarya were located close to the paired prehypophysial arteries near the anterior end of the postoptic nucleus. In the neurohypophysis, abundant presumptive AVT-fibers terminated in the posterior dorsal wall, although some fibers terminated in the anterior dorsal wall and only a few fiber endings were found in the ventral wall. No ACTH-positive cells were detected in the hagfish brain or in the pituitary gland.
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    The immunocytochemical localization of pituitary somatotrops in the genus Oncorhynchus using an antiserum to growth hormone of chum salmon (Oncorhynchus keta) (1983)
    Springer
    Cell & tissue research 231 (1983), S. 693-697 
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    ISSN: 1432-0878
    Keywords: Somatotrops ; Oncorhynchus ; Immunocytochemistry ; Immunodiffusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitary somatotrops were localized in four species from the genus Oncorhynchus using an antiserum to chum salmon (Oncorhynchus keta) growth hormone. The antiserum cross-reacted specifically with the acidophils in the caudal pars distalis of all species tested. Corroborative immunodiffusion studies were also conducted with pituitary homogenates from the same species. Immunological identity of the GH molecule was observed for all species tested.
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    Changes in hypothalamic and extra-hypothalamic vasopressin content of water-deprived rats (1983)
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    Cell & tissue research 233 (1983), S. 99-111 
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    ISSN: 1432-0878
    Keywords: Neurosecretion ; Vasopression ; Osmotic stress ; RIA ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A correlative radioimmunoassay (RIA) and immunocytochemical (ICC) study was carried out on vasopressin (VP) distribution and content in brains of normal and 3-day water-deprived rats. By RIA there were statistically significant differences in brain VP per pg/mg between normal and osmotically stressed specimens in hypothalamus (338.4 versus 134.4), thalamus (4.8 versus 0.9), septum (18.0 versus 3.4), striatum (1.6 versus 0.7) and amygdala (17.3 versus 1.3), but not in other brain regions measured. Pituitary VP decreased from 71.1 to 8.7 ng/mg, and plasma VP rose from 3.6 to 19.3 pg/ml during water deprivation. Application of the peroxidase-anti-peroxidase ICC method of Sternberger to vibratome sections showed that VP-immunoreactivity in dehydrated specimens decreased in perikarya of paraventricular nucleus and suprachiasmatic nucleus, while intrahypothalamic immunoreactive magnocellular fibers appeared more conspicuous due to proliferation of large Herring bodies. In extrahypothalamic sites VP-immunoreactivity in water-deprived rats was visibly reduced in periventricular thalamus and septum. Thus it is apparent that both intra- and extrahypothalamic VP are affected by osmotic stress, and these results are discussed within the context of current ideas relating to co-activation of neurosecretory cells that project to different sites.
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    Light-microscopic immunocytochemical localization of fibronectin in the developing rat lung (1983)
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    Cell & tissue research 233 (1983), S. 113-123 
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    ISSN: 1432-0878
    Keywords: Lung development ; Fibronectin ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the rat lung is a process of continuing morphological change. Indications from work in other mammalian systems suggest that fibronectin may be important in the control of this process. The present study has examined embryonic, neonatal, and adult lung tissue of the rat by means of the peroxidase-antiperoxidase (PAP) technique to demonstrate fibronectin at the light-microscopic level. Positive reaction was observed with anti-fibronectin serum in all stages examined. Control sections treated with pre-immune serum or no primary serum gave negative results in each case. Fibronectin in adult tissue was localized to the alveolar surface and alveolar basal lamina. Neonatal tissue showed fibronectin on pulmonary tubule walls and in basal lamina while embryonic tissue revealed localization of the protein in the basal lamina and in association with small groups of cells at the base of septal buds. These findings suggest a role for fibronectin in the control of rat lung development. The results are discussed in terms of the known functions of fibronectin as a preliminary matrix for the subsequent deposition of collagenous connective tissue, as a cellular adhesion protein, and as surface-bound material for cellular migration.
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    Cytological localization of α-MSH, ACTH and β-endorphin in the pars intermedia of the cichlid teleost Sarotherodon mossambicus (1981)
    Springer
    Cell & tissue research 215 (1981), S. 625-633 
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    ISSN: 1432-0878
    Keywords: Teleost ; Pars intermedia ; Immunocytochemistry ; PAP-technique ; MSH ; ACTH ; Endorphin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pars intermedia of S. mossambicus contains two different endocrine-cell types. The predominant cell type is lead-haematoxyline-positive and assumed to synthesize MSH and related peptides. The second cell type is PAS positive and its function and product(s) are unknown. Staining of light-microscopic and ultrathin sections with antisera against α-MSH, ACTH 1–24 and human β-endorphin revealed that only the lead-haematoxyline-positive cells of the pars intermedia react with these antisera, and that the secretory granules of these cells contain compounds that were immunoreactive to all three antisera. These findings are in line with the hypothesis that α-MSH, ACTH and endorphins are derived from the same precursor molecule. No specific reaction with one of the antisera could be detected in the PAS positive cells.
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    Calcium-binding protein in the developing rat cerebellum (1983)
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    Cell & tissue research 233 (1983), S. 389-402 
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    ISSN: 1432-0878
    Keywords: Calcium-binding protein (CaBP) ; Immunocytochemistry ; Rat cerebellum ; Development ; Vitamin D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specific antibodies raised against a human 28 000 dalton cerebellar calcium-binding protein (CaBP) were used in an immunocytochemical study during development of the rat cerebellum. Both light and electron microscopy showed (1) that labelling was entirely restricted to the Purkinje cells, (2) that it appeared very early in Purkinje cell development, (3) that the entire cell was labelled from the tip of the smallest dendrites to the axonal terminals, and (4) that with increasing age, the immunoreaction appeared to be progressively restricted to the cell and organelle membranes.
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    URL: http://dx.doi.org/10.1007/BF00238305
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    Immunocytochemical demonstration of peptidergic neurons in the central nervous system of the pond snail Lymnaea stagnalis with antisera raised to biologically active peptides of vertebrates (1981)
    Springer
    Cell & tissue research 216 (1981), S. 273-291 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Biologically active peptides ; Lymnaea stagnalis ; Neurohormone ; Neurotransmitter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Perikarya and nerve fibers were immunocytochemically identified in the central nervous system of the pond snail Lymnaea stagnalis by means of the unlabelled antibody enzyme method with antisera to 15 biologically active peptides of vertebrates: vasopressin, vasotocin, oxytocin, α-melanocyte stimulating hormone (α-MSH), met-enkephalin, somatostatin, glucagon, insulin, glucose-dependent insulinotropic peptide (GIP), vaso-active intestinal polypeptide (VIP), gastrin, secretin, pancreatic polypeptide (PP), Substance P, calcitonin. No immunostaining was obtained with antisera to β-endorphin, cholecystokinin (CCK), neurophysin I and II. Particular neurons could be identified with two antisera (anti-vasopressin/vasotocin, anti-α-MSH/metenkephalin, anti-substance P/PP, anti-PP/gastrin). Apparently this indicates that populations of cells identified with a given antiserum may consist of more than one cell type. Only a few of the new peptidergic cells appeared to be identical with classical neurosecretory cells. Thus the growth hormone producing Light Green Cells stained with anti-somatostatin and the axon terminals of the ovulation hormone producing Caudo-Dorsal Cells with anti-met-enkephalin. Whether this indicates structural identity of the growth hormone with somatostatin and of the ovulation hormone with met-enkephalin remains to be investigated. Just like the classical neurosecretory cells a number of the new peptidergic cells (anti-glucagon, -insulin, -met-enkephalin, -somatostatin, and -PP positive cells) send their axons to the peripheries of commissures, connectives or nerves. Thus these cells can be considered as probably neuroendocrine. The classical neurosecretory cells release their products into the haemolymph from these sites. Other new peptidergic cells (e.g., anti-vasopressin, -vasotocin, -oxytocin and -GIP positive cells) have axons that terminate, probably synaptically, on other neurons, indicating that they are “more conventional” neurons, their products being neurotransmitters/neuromodulators. It can also not be excluded that some cells of a population containing a given peptide are neuroendocrine and others make contact with other neurons.
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    URL: http://dx.doi.org/10.1007/BF00233620
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    Evidence for somatostatin, gastrin and pancreatic polypeptide-like substances in the mucosa cells of the gut in fishes with and without stomach (1981)
    Springer
    Cell & tissue research 216 (1981), S. 193-203 
    Details
    ISSN: 1432-0878
    Keywords: Digestive tract ; Fish ; Gastrin-immunoreactive cells ; Pancreatic polypeptide-immunoreactive cells ; Somatostatin-immunoreactive cells ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gastrin, pancreatic polypeptide and somatostatin immunoreactive cells in the gut of two fish with stomachs (perch and catfish) and a stomachless fish (carp) were studied by immunocytochemistry. In the gastric mucosa of perch and catfish, cells showing gastrin and somatostatin-like immunoreactivity are found, scattered among the surface mucous cells and mucous neck cells. No pancreatic polypeptide (P.P.) immunoreactive cells are detected in the gastric mucosa. Cells showing gastrin and P.P.-like immunoreactivity are observed in the intestinal mucosa of perch, catfish and carp. In this location no somatostatin immunoreactive cells are found.
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    URL: http://dx.doi.org/10.1007/BF00234554
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    Relationship of the central ACTH-immunoreactive opiocortin system to the median eminence and the pituitary gland of the rat (1981)
    Springer
    Cell & tissue research 215 (1981), S. 333-340 
    Details
    ISSN: 1432-0878
    Keywords: ACTH-opiocortin system ; Immunocytochemistry ; Median eminence ; Neural lobe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By immunocytochemical methods, the present study describes ACTH-immunoreactive fibers in the pituitary stalk and neural lobe. This opiocortin-hypothalamo-neural lobe projection arises in a bed nucleus of perikarya in the basal hypothalamus, follows supraoptico-hypophyseal fibers in the zona interna of the median eminence, and distributes throughout the neural lobe. No ACTH-immunoreactive fibers project to the zona externa; some are present in the subependymal layer and at the lateral margins of the median eminence. Further studies must identify the role of these fibers in posterior lobe function. It remains also to be determined whether this system terminates upon primary pituitary portal capillaries and delivers opiocortin neuropeptides to the adenohypophysis.
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    URL: http://dx.doi.org/10.1007/BF00239119
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    Immunocytochemical identification of proctolinlike immunoreactivity in the terminal ganglion and hindgut of the cockroach Periplaneta americana (L.) (1981)
    Springer
    Cell & tissue research 217 (1981), S. 633-645 
    Details
    ISSN: 1432-0878
    Keywords: Proctolin ; Periplaneta americana ; Terminal ganglion-hindgut system ; Immunocytochemistry ; Cobalt iontophoresis technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the American cockroach, the distribution and connections of neuronal elements of the terminal ganglion-proctodeal nerve-hindgut system were investigated by means of immunohistochemical methods and axonal CoCl2 iontophoresis. Proctolinlike immunoreactivity was localized within neurons of the terminal ganglion projecting into the proctodeal nerve on the one hand, and in nerve cells without a direct connection to this system on the other. Immunohistochemically, in whole mount preparations fibres of the proctodeal nerve and terminal structures in the hindgut musculature exhibit strong proctolinlike immunoreactivity. At the light- and electron-microscopic levels the pathways of about 30 somata of the proctodeal neural system were characterized by cobalt chloride iontophoresis. The relationships of cobalt filled and immunoreactive neuronal structures are discussed. For the preparation of tritiated proctolin we thank Dr. S. Reißmann, WB Biochemie, Sektion Biologie, FSU Jena
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    URL: http://dx.doi.org/10.1007/BF00219370
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    Ultrastructural immunocytochemical evidence of the presence of a peptide related to ACTH in granules of LHRH nerve terminals in the median eminence of the guinea pig (1981)
    Springer
    Cell & tissue research 218 (1981), S. 1-6 
    Details
    ISSN: 1432-0878
    Keywords: 17–39 ACTH ; LHRH terminals ; Median eminence ; Guinea pig ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary At the ultrastructural level a peptide immunocytochemically related to ACTH was localized in LHRH-containing terminals and nerve fibers of the palisade zone in the median eminence of the guinea pig. Precise comparisons of adjacent ultrathin sections stained for 17–39 ACTH and for LHRH made it possible to state clearly that in the two cases the staining was confined to granules containing both peptides. The significance of such an association remains open to discussion.
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    URL: http://dx.doi.org/10.1007/BF00210085
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    Pea seed storage proteins — Immunocytochemical localization with protein a-gold by electron microscopy (1981)
    Springer
    Protoplasma 105 (1981), S. 333-339 
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    ISSN: 1615-6102
    Keywords: Immunocytochemistry ; Pea seed ; Protein histochemistry ; Storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pea seed storage proteins legumin and vicilin have been localized by electron microscopy using a post-embedding immunocytochemical double-labelling technique. Highly purified antibodies from sheep, specific to legumin and vicilin, were sequentially reacted on sections of pea cotyledon tissue embedded in glycol methacrylate or Spurr's epoxy resin. The sheep antibodies were visualized indirectly by reaction with Staphylococcal Protein A labelled with colloidal gold. Two discrete sizes of colloidal gold particles can be used for simultaneous localization of two antigens. In near mature tissue, the storage proteins are restricted to organelles, called protein bodies. Individual protein bodies contain both legumin and vicilin.
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    URL: http://dx.doi.org/10.1007/BF01279230
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    Localization of relaxin in human gestational corpus luteum (1981)
    Springer
    Cell & tissue research 219 (1981), S. 213-216 
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    ISSN: 1432-0878
    Keywords: Relaxin ; Human pregnancy ; Corpus luteum ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Corpora lutea from 12 pregnant women were prepared for immunohistochemical localization of relaxin using a highly specific antiserum. A positive response is given by luteal cells that are diffusely distributed throughout the corpus luteum. These cells do not form a distinctive group in any particular area. A negative response is seen in the adjacent ovarian tissue, and also in nongestational corpora lutea in an early luteal phase.
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    URL: http://dx.doi.org/10.1007/BF00210030
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    Immunocytochemical demonstration of a humoral defence factor in blood cells (amoebocytes) of the pond snail, Lymnaea stagnalis (1981)
    Springer
    Cell & tissue research 219 (1981), S. 291-296 
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    ISSN: 1432-0878
    Keywords: Opsonin ; Agglutinin ; Amoebocytes ; Immunocytochemistry ; Lymnaea stagnalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monolayers of blood cells (amoebocytes) and sections of connective tissue and of amoebocyte pellets of the freshwater pulmonate gastropod Lymnaea stagnalis were stained immunocytochemically with antisera to snail agglutinin/opsonin. The presence of this substance was demonstrated light microscopically both in the cytoplasm and on the cell membrane of amoebocytes. This suggests that amoebocytes synthesize agglutinin/opsonin, and bear it at their surface as receptors for foreign materials.
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    URL: http://dx.doi.org/10.1007/BF00210149
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    Immunocytochemistry of luteinizing hormone-releasing hormone, vasopressin, and corticotropin following deafferentation of the basal hypothalamus of the male rat brain (1981)
    Springer
    Cell & tissue research 219 (1981), S. 393-402 
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    ISSN: 1432-0878
    Keywords: Hypothalamic deafferentation ; Immunocytochemistry ; Vasopressin ; ACTH/opiocortin ; LHRH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Luteinizing hormone-releasing hormone (LHRH), vasopressin, and corticotropin systems were examined by immunocytochemical methods in male rats 2 to 20 days after deafferentation of the basal hypothalamus. Axonal degeneration of the vasopressin system (whose perikarya lie rostral to the island) and the corticotropin system (whose perikarya lie within the island) was examined and compared with the response of the LHRH system. Vasopressin immunoreactive staining was absent in the internal zone of the median eminence 10 and 20 days after deafferentation. Disruption of the efferent projections of the opiocortin system caused the loss of almost all fiber staining outside the island by the 5th postoperative day. LHRH staining in the median eminence was modestly reduced in 5 days, considerably reduced in 10 days and negligible 20 days after deafferentation. At 10 and/or 20 days after deafferentation densely stained fibers of all three systems were observed on both sides of the cut. Invasive vasopressinergic fibers reached the lateral median eminence by the 20th postoperative day. This study reports on the response of three neuropeptide systems after complete deafferentation and demonstrates that regeneration can occur across the knife cut.
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    URL: http://dx.doi.org/10.1007/BF00210157
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    Localization of gonadotrophic hormones in the pituitary gland of the California leaf-nosed bat (Macrotus californieus) (1981)
    Springer
    Cell & tissue research 220 (1981), S. 115-123 
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    ISSN: 1432-0878
    Keywords: Pars distalis ; California leaf-nosed bat ; Macrotus californicus ; LH ; FSH ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Luteinizing hormone (LH) and follicle-stimulating hormone (FSH) cells were immunostained in the anterior pituitary lobe of the California leaf-nosed bat with antisera against the specific beta (β) subunits of rat LH and rat FSH employing the unlabeled immunoperoxidase technique. Selective immunochemical staining by both antisera was localized primarily in the same cell type. However, some cells consistently reacted solely with antisera to either LH-β or FSH-β. Whereas the immunoreactive LH-β cells, in general, stained more intensely than FSH-β cells they were usually less numerous. These cells were alcian-blue and PAS-positive, staining pink to purple. The LH/FSH cells were pleomorphic with eccentric nuclei, often with cytoplasmic extensions. They were found throughout the pars distalis.
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    URL: http://dx.doi.org/10.1007/BF00209970
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    Immunocytochemical devidence for hypophysial projections of the cells of the nucleus lateralis tuberis pars lateralis in the rainbow trout (Salmo gairdneri) (1983)
    Springer
    Cell & tissue research 228 (1983), S. 43-50 
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    ISSN: 1432-0878
    Keywords: Nucleus lateralis tuberis ; Hypothalamo-hypophysial relationship ; Immunocytochemistry ; Prolactin cells ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the rainbow trout the pars lateralis is the most prominent part of the nucleus lateralis tuberis (NLT). To demonstrate a morphological relationship between this lateral part of the NLT and the pituitary, immunocytochemistry was applied as a staining method. Experiments were carried out on glutaraldehyde-picric acid-acetic acidfixed brain sections of mature male and female rainbow trout using the peroxidase-anti-peroxidase immune technique with an antiserum against 27-S-methylglucagon as the first antibody. Most of the cells in the NLT/pars lateralis reacted with the antiserum. Axons from these cells enter the pituitary, extending exclusively in the numerous neurohypophysial digitations in the pars intermedia. No immunoreactive neurohypophysial protrusions were found in those parts of the adenohypophysis where the gonadotropic cells are located, indicating that the lateral part of the NLT is not directly involved in the control of gonadotropin secretion. In addition to cells of the NLT/pars lateralis only prolactin cells in the rostral pars distalis of the adenohypophysis reacted with the antiserum used.
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    URL: http://dx.doi.org/10.1007/BF00206263
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    Vasopressin-immunoreactive cell bodies in the bed nucleus of the stria terminalis of the rat (1983)
    Springer
    Cell & tissue research 228 (1983), S. 525-534 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Vasopressin ; Oxytocin ; Bed nucleus, stria terminalis ; Lateral septum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the dorsal and ventral portions of the bed nucleus of the stria terminalis of the rat numerous cell bodies immunoreactive for vasopressin and neurophysin II were found after colchicin pretreatment. These cells are predominantly multipolar but sometimes also bipolar, and have a width and length of approximately 9 and 16 μm, respectively. In the homozygous Brattleboro rat, which is deficient in vasopressin, no immunoreactive vasopressin was found in these cells. Following incubation with anti-oxytocin and anti-bovine neurophysin I, only magnocellular immunoreactive cell bodies were found in the septal region. The consequences of these results concerning the vasopressin fiber pathways in the brain are discussed.
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    URL: http://dx.doi.org/10.1007/BF00211473
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    Immunocytochemical localization of TRH and autoradiographic determination of 3H-TRH-binding sites in the arcuate nucleus-median eminence of the rat (1983)
    Springer
    Cell & tissue research 228 (1983), S. 475-487 
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    ISSN: 1432-0878
    Keywords: TRH ; Immunocytochemistry ; Autoradiography ; Arcuate nucleus ; Median eminence ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical and autoradiographic localization of thyrotropin-releasing hormone (TRH)-and 3H-TRH-binding sites was studied in the arcuate nucleus-median eminence region of the rat. TRH-like immunoreactivity was found in dense granular vesicles (90–140 nm in diameter) in TRH-like immunoreactive nerve fibers and terminals. In the median eminence, the immunoreactive terminals were observed to be in direct contact with the perivascular basal lamina of the portal vessel and to form synaptoid contacts with tanycytes. In the arcuate nucleus, the immunoreactive terminals were often found to form axosomatic and axo-axonic, and/or axo-dendritic synapses. The uptake of tritiated TRH into the nerve fibers and terminals of the median eminence was also observed by autoradiography and the distribution and localization of silver grains in them were analyzed quantitatively by circle analysis. Thirty minutes after intraventricular infusion of 3H-TRH, radioactive labeling occurred in type-2 and 3-nerve fibers and terminals containing dense granular vesicles in the median eminence. It is therefore suggested that the neurons labeled after 3H-TRH infusion possess certain functions as physiological recognition sites or receptors for TRH.
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    URL: http://dx.doi.org/10.1007/BF00211469
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    Adipokinetic hormone and AKH-like peptide demonstrated in the corpora cardiaca and nervous system of Locusta migratoria by immunocytochemistry (1983)
    Springer
    Cell & tissue research 230 (1983), S. 67-76 
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    ISSN: 1432-0878
    Keywords: Adipokinetic hormone ; Locusta migratoria ; Neurohormone ; Peptidergic neuron ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antiserum was raised against tyrosine-adipokinetic hormone ([Tyr1]-AKH). In immunohistochemical procedures, it revealed the AKH cells in the glandular lobes of the corpora cardiaca (CC) of Locusta migratoria with high specificity. In addition, an immunologically related peptide was detected in certain neurons of the central nervous system which suggests that this AKH-like peptide may have a neurotransmitter function. The glandular lobes contain immunoreactive AKH cells in all postembryonic stages, and no essential differences in morphology and distribution of the cells in nymphs and adults were seen. The amount of AKH, stored predominantly in the cell projections, differs widely among cells and individuals. The brain of adults and nymphs contains several small populations of intensely stained neurons. In last-instar and adult specimens, each half contains 10–12 “normal”-sized neurons in the protocerebrum (including the optic lobe) and deutocerebrum, and in addition 15–18 small reactive neurons. Their axons and numerous branchings traverse the neuropile of proto-, deuto-, and tritocerebrum, except for the pedunculate bodies and antennal lobes. Some of the axons run into the storage lobe of the CC; it is unknown if their content is released into the haemolymph. Other axons run into the ganglia of the stomatogastric nervous system and into the circumoesophageal connectives. The suboesophageal ganglion also contains 8 immunoreactive neurons. It is unknown to which extent the immunoreactive substances in glandular and nervous tissue are chemically and physiologically related.
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    URL: http://dx.doi.org/10.1007/BF00216028
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    Histophysiological and immunocytochcmical study on the nature of the thyrotrops in the pituitary of immature rainbow trout, Salmo gairdneri (1983)
    Springer
    Cell & tissue research 231 (1983), S. 185-198 
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    ISSN: 1432-0878
    Keywords: Thyroid ; Pituitary ; Thyrotrops ; Gonadotrops ; Immunocytochemistry ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Alcian blue and periodic acid-Schiff-positive “granular basophils” with electron-dense granules ±160 nm in diameter, and weakly developed irregular cisternae of the granular endoplasmic reticulum (GER) are present in the rostral pars distalis (RPD) and the proximal pars distalis (PPD) of immature rainbow trout. They all react with antisalmon αβ-gonadotropin. However, only granular basophils in the caudal RPD and the rostro-dorsal PPD, often bordering on and sometimes surrounded by neurohypophysial tissue, react with anti-human β-TSH. These cells, considered as the source of thyrotropin, show degranulation and dilatation of the GER-cisternae in fish treated with potassium perchlorate. The thyroids of the goitrogen-treated animals had relatively numerous small follicles with a high epithelium. The remaining granular basophils are gonadotrops.
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    URL: http://dx.doi.org/10.1007/BF00215785
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    Immuno-electron-microscopic evidence of ecdysteroids in Y-organ of Orconectes limosus (Crustacea, Decapoda) (1983)
    Springer
    Cell & tissue research 233 (1983), S. 233-236 
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    ISSN: 1432-0878
    Keywords: Y-organ ; Steroid secretion ; Ecdysone ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ecdysone was demonstrated by ultrastructural immunocytochemistry to be present in the mitochondria of the Y-organs of the crayfish Orconectes limosus. This is in remarkable contrast to the prothoracic glands of insects and suggests substantial differences in the biosynthesis of the same hormone, ecdysone, in crustaceans and insects.
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    URL: http://dx.doi.org/10.1007/BF00222247
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    Presence of β-endorphin-like immunoreactivity in the anterior pituitary gland of rat and man and evidence for the differential localization with ACTH (1981)
    Springer
    Cell & tissue research 215 (1981), S. 577-589 
    Details
    ISSN: 1432-0878
    Keywords: Anterior pituitary ; Corticotrophs ; Immunocytochemistry ; Growth hormone ; Prolactin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two antisera, Y-10 and Y-18 were raised in rabbits against synthetic human β-endorphin conjugated to bovine serum albumin and keyhole limpet haemocyanin respectively. Antiserum Y-10 has been shown by radioimmunoassay to be highly specific for human β-endorphin with minimal or no cross-reactivity against other pituitary peptides whilst antiserum Y-18 crossreacted on an equimolar basis against β-endorphin and β-lipotropin. When used in the immunohistochemical procedure, both antisera specifically stained the corticotrophs in human anterior pituitary tissue. A similar effect was observed when antiserum Y-18 was applied to rat anterior pituitary tissue in the immunohistochemical procedure. Y-10 antiserum, on the other hand, stained not only rat corticotrophs but also somatotrophs. The somatotrophin staining could not be attributed to the enkephalins reported to be present in these cells. The non-specific β-endorphin antiserum Y-18 was used to stain anterior pituitaries from dehydrated and adrenalectomized rats as well as rats of the Brattleboro strain. In tissues from the three experimental animals, cells that stained positively for β-endorphin did not give a positive immunoreaction for ACTH and vice versa in some other sections. It is concluded that under the physiological conditions, formalin fixation of the tissue causes the proopiocortin molecule to be “trapped” in a conformation such that either ACTH or β-endorphin-like determinants are available for reacting with the appropriate antiserum.
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    URL: http://dx.doi.org/10.1007/BF00233533
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    Ultrastructural identification of corticotropes of the fetal rat (1983)
    Springer
    Cell & tissue research 234 (1983), S. 427-437 
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    ISSN: 1432-0878
    Keywords: Corticotropes ; Rat fetus ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Corticotropes of rat fetuses aged 16, 18 and 21 days were localized by the indirect antibody-enzyme method on semithin sections of the pituitary. The development of the ultrastructure of these cells was observed on consecutive ultrathin sections. In comparison with previous data our present results show that identification of a fetal cell type cannot be based entirely on morphological criteria. The structural peculiarities of corticotropes obtained from studies in vivo are compared with those observed in cells maintained in vitro.
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    URL: http://dx.doi.org/10.1007/BF00213779
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    Immunoreactive substance P in the chicken gut: Distribution, development and possible functional significance (1981)
    Springer
    Cell & tissue research 216 (1981), S. 455-469 
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    ISSN: 1432-0878
    Keywords: Neuropeptides ; Substance P ; Chicken gut ; Radioimmunoassay ; Immunocytochemistry ; Smooth muscle motility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution and cellular localization of substance P in the chicken gut was studied by immunocytochemistry and immunochemistry. Substance P-containing nerve fibers are numerous in the gut wall. They occur in the smooth muscle layer as well as in the mucosa, where they are associated with blood vessels or surround the intestinal crypts. The fibers are particularly numerous in the myenteric and submucosal plexuses, where substance P-containing nerve-cell perikarya are also encountered. Substance P was found also in scattered endocrine cells of the small intestine, caeca and colon. Previously, bombesin-containing cells, which are numerous in the proventriculus, have been mistakenly identified as substance P cells due to crossreactivity of certain antisera against substance P. Immunochemistry revealed the highest concentration of substance P in the duodenum. The gel chromatographic behavior of chicken substance P differs slightly from that of synthetic bovine substance P, suggesting that chicken substance P differs structurally from mammalian substance P. Substance P-containing nerve fibers in the chicken gut develop slowly after hatching, apparently beginning in the duodenum; at approximately 20 weeks after hatching the distribution pattern is fully developed. A functional investigation was performed on the isolated chicken caecum to clarify the role of substance P in the contractile behavior of smooth muscle. Substance P contracted the caecum over a wide dose range; the contractile response was greater in 20 week-old chickens than in 4 and 10 week-old animals. Electrical field stimulation caused a relaxation of the caecum and a contraction upon cessation of stimulation. Neither of these responses, both of which are neurally mediated, were inhibited by adrenergic and cholinergic blockade. It is conceivable that the contractile response following electrical stimulation is caused by substance P released from nerve fibers in the smooth muscle.
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    Evidence for the association of villin with core filaments and rootlets of intestinal epithelial microvilli (1983)
    Springer
    Cell & tissue research 228 (1983), S. 409-414 
    Details
    ISSN: 1432-0878
    Keywords: Villin ; Intestinal brush border ; Contractile proteins ; Immunocytochemistry ; Immunostaining of blotted gels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Villin, a 95,000 dalton polypeptide of intestinal brush border which is known to bundle or sever actin filaments in a Ca++-dependent manner, was localized in rat and chicken intestinal epithelium by means of immunocytochemistry at the light- and electron-microscopic levels. Specific antibodies to villin were raised in rabbits immunized with villin purified from chicken intestinal epithelium. Anti-villin bound selectively to the microvillus filament bundle from its tip down to the rootlets. These findings indicate that the well-known stability of rootlet filaments towards elevated Ca++ ion concentrations cannot be explained by the absence of villin. Therefore additional factors must exist which prevent the rootlets from Ca++-villin mediated disassembly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204889
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    The duct-ligated pancreas transplant and its effect on the islet cellular composition of the host pancreas (1983)
    Springer
    Cell & tissue research 231 (1983), S. 205-213 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Insulin ; Glucagon ; Pancreatic polypeptide ; Diabetes mellitus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rats rendered diabetic by streptozotocin were subjected to pancreas transplantation. After twenty weeks, the duct-ligated pancreas transplant was studied morphometrically to determine the effect of duct occlusion on the various cell populations of the islets. Concomitantly, the streptozotocin-treated host pancreas was examined for a possible influence of the graft on the diabetic pattern of islet cell population. Twenty weeks after pancreas transplantation, the volume fractions of insulin, glucagon, somatostatin and pancreatic polypeptide cells in the graft islets did not differ from those of the normal control pancreas. In the pancreas of nontransplanted diabetic rats, insulin-positive B cells were reduced from 60–65% to less than 10% of the islet volume, whereas non-B cells were significantly increased in volume density. The changes in fractional volume of the various islet cells correlated fairly well with changes in plasma concentration of the corresponding pancreas hormones. In the recipient's own pancreas, the relative volumes of glucagon and somatostatin cells were unaffected by the pancreas transplant. However, the insulin cell mass was significantly increased, and comprised about 20% of the islet volume, while cells containing pancreatic polypeptide were found only sporadically.
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    URL: http://dx.doi.org/10.1007/BF00215787
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    Pituitary folliculo-stellate cells immunostained with S-100 protein antiserum in postnatal, castrated and thyroidectomized rats (1983)
    Springer
    Cell & tissue research 231 (1983), S. 235-249 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; S-100 protein ; Castration ; Thyroidectomy ; Postnatal development ; Immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitary folliculo-stellate cells and associated cells were studied immunohistochemically throughout the life of male and female rats. The marginal layer cells of the pars distalis and intermedia as well as the folliculo-stellate cells were immunostained with anti-S 100 protein serum. The immunostained folliculo-stellate cells in one section were identified as themselves by their ultrastructural properties on the adjacent section. Corticotrophs, thyrotrophs and prolactin cells, characterized by the stellate shape, were not immunostained with anti-S 100 protein serum. Reactivity for S-100 protein appeared on postnatal Day 6 in the marginal layer cells of the pars intermedia, and appeared on Day 10 in those cells of the pars distalis. No immunostained folliculostellate cells appeared before Day 6, but a few of them appeared on Day 10. Thereafter they increased in number, having more intense immunostaining with advancing age. In castrated rats, the immunostained folliculo-stellate cells appeared quite numerously, with branched cytoplasmic processes surrounding the gonadotrophs. In the thyroidectomized rats, however, folliculo-stellate cells lacked ramified cytoplasmic processes, and their topographic affinity for thyrotrophs was negligible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222177
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    An immunocytochemical study on the localization of S-100 protein in the retina of rats (1983)
    Springer
    Cell & tissue research 231 (1983), S. 527-532 
    Details
    ISSN: 1432-0878
    Keywords: S-100 ; Müller cells ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of S-100 protein in the retina was studied by immuno-electron microscopy. The immunoreaction for S-100 is specifically confined to Müller cells and astrocytes. Long slender cytoplasmic processes of Müller cells and their participation in the formation of the inner and outer limiting membrane were clearly demonstrated with this immunoreaction. The cytoplasmic processes were also revealed to form continuous sheets surrounding blood vessels. Some somata of Müller cells were found in the outer nuclear layer. The results show that immunostaining for S-100 is useful for selective visualization of Müller cells in the mature retina.
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    URL: http://dx.doi.org/10.1007/BF00218111
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  • 93
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    Topographical distribution of the gonadotrophs, mammotrophs, somatotrophs and thyrotrophs in the pituitary gland of the baboon (Papio cynocephalus) (1983)
    Springer
    Cell & tissue research 230 (1983), S. 233-238 
    Details
    ISSN: 1432-0878
    Keywords: Baboon ; Pituitary gland ; Gonadotrophs ; Mammotrophs ; Somatotrophs ; Thyrotrophs ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The identification and regionalization of four pituitary parenchymal cell types, gonadotrophs, mammotrophs, somatotrophs and thyrotrophs, were studied in the baboon (Papio cynocephalus) hypophysis using immunocytochemistry. The gonadotrophs were homogeneously distributed throughout the entire pars distalis. Both mammotrophs and somatotrophs predominate at the superior and inferior poles of the organ. The medial and anteromedial regions are populated by mammotrophs and thyrotrophs, while the lateral and posterior portions of the pars distalis contain large numbers of somatotrophs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216044
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  • 94
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    Immunocytochemical identification of thyrotropin (TSH)-producing cells in pituitary glands of several species of teleosts with antiserum to human TSH β subunit (1983)
    Springer
    Cell & tissue research 231 (1983), S. 199-204 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Human TSH β subunit ; Teleosts ; TSH cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A rabbit antiserum raised against human thyrotropin (TSH) β subunit (anti-human β-TSH) was used to identify TSH cells in the pituitaries of five species of teleosts by means of the unlabeled antibody peroxidase-antiperoxidase (PAP) technique. The anti-human β-TSH reacted only with polygonal basophils located in the rostral pars distalis (RPD) in the pituitaries of sexually immature eel (Anguilla japonica). In the pituitaries of medaka (Oryzias latipes) and white-edged rockfish (Sebastes taczanowskii), this serum reacted specifically with only one cell-type located in the antero-dorsal proximal pars distalis (PPD) and antero-ventral PPD, respectively. In the pituitaries of two species of salmonids, sexually immature rainbow trout (Salmo gairdneri) and sexually mature chum salmon (Oncorhynchus keta), the anti-human β-TSH bound to the polygonal basophils in the ventral PPD adjacent to the RPD. Thus, the present immunocytochemical study identified polygonal basophils located in the RPD or PPD as TSH cells in five species of teleosts.
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    URL: http://dx.doi.org/10.1007/BF00215786
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  • 95
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    Serotonin-like immunoreactivity in the optic lobes of three insect species (1983)
    Springer
    Cell & tissue research 232 (1983), S. 129-140 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Optic lobes ; Insecta ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cellular localization of 5-HT in the optic lobes of three insect species was assayed with the use of antibodies raised against 5-HT. In Schistocerca, Periplaneta, and Calliphora all neuropil regions of the optic lobe, the lamina, medulla and lobula, contain 5-HT-immunoreactive varicose fibres in different patterns, like columns and layers. Such fibres also connect the lobula to neuropil in the lateral protocerebrum. In Calliphora also 5-HT-positive fibres of the medulla and lobula plate have projections to the lateral protocerebrum, whereas the origin of the lamina fibres is not certain. In all species the processes displaying 5-HT-like immunoreactivity appear to be derived from a relatively small number of cell bodies, each neuron thus having processes over a large volume of the neuropil of the optic lobe in different layers.
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    URL: http://dx.doi.org/10.1007/BF00222379
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  • 96
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    Vasoactive intestinal polypeptide (VIP)-like immunoreactivity in anuran intestine (1981)
    Springer
    Cell & tissue research 216 (1981), S. 413-422 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Vasoactive intestinal polypeptide ; Radioimmunoassay ; Gut hormones ; Anura
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical and radioimmunological techniques with region specific antisera have been used to identify a vasoactive intestinal polypeptide-like material in the anuran intestine. Seven species of Anura were investigated: Bombina bombina, Alytes obstetricans, Rana temporaria, Rana esculenta, Hyla arborea, Hyla crepitans and Bufo bufo. In five of the species (A. obstetricans, R. temporaria, H. arborea, H. crepitans and B. bufo) vasoactive intestinal polypeptide-like immunoreactive mucosal endocrine cells and nerve fibres in all layers of the gut wall, were detected by both immunofluorescence and peroxidase-antiperoxidase methods. In the other two species, R. esculenta and B. bombina, no mucosal endocrine cells were detected although the vasoactive intestinal polypeptide-immunoreactive nerve fibres were plentiful. Radioimmunoassay showed the presence of significant amounts of vasoactive intestinal polypeptide-immunoreactivity in intestinal extracts from all species. The highest quantities were present in those anurans with both immunostained cells and nerves. Gel permeation chromatography showed that most of the vasoactive intestinal polypeptide-like peptide eluted in a position identical to that of natural mammalian (porcine) vasoactive intestinal polypeptide. The results indicate that a vasoactive intestinal polypeptide-like peptide is well represented in the Anura and that it is immunologically very similar to the mammalian peptide.
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    URL: http://dx.doi.org/10.1007/BF00233628
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    Immunocytochemical demonstration of the transport of myelin proteolipids through the Golgi apparatus (1983)
    Springer
    Cell & tissue research 234 (1983), S. 547-559 
    Details
    ISSN: 1432-0878
    Keywords: Myelin proteolipids ; Oligodendrocytes ; Golgi apparatus ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Purified antibodies directed against myelin proteolipids were isolated by affinity chromatography of whole serum obtained from rabbits inoculated with myelin. These antibodies were specific for light, medium and dark oligodendrocytes. Astrocytes, neurons and their processes were not reactive. Immunocytochemical investigations showed that the membranes of the Golgi complex are highly labeled by these antibodies. Diffuse cytoplasmic labeling was only observed on the light and medium oligodendrocytes and was absent from the dark types. Vesicles possessing a punctate staining were detected in the vicinity of the Golgi complex and the oligodendroglial membrane. A discontinuous labeling of the plasmalemma appears to be characteristic of the actively myelinating light and medium oligodendrocytes. In compact myelin sheaths positive immunostaining was only detected at the dense line. The immunocytochemical localization of the myelin proteolipids in the oligodendrocytes is in accordance with previously published biochemical data.
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    URL: http://dx.doi.org/10.1007/BF00218650
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    Distribution of immunoreactive somatostatin in the primate hypothalamus (1983)
    Springer
    Cell & tissue research 233 (1983), S. 69-80 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin ; Hypothalamus ; Immunocytochemistry ; Human ; Rhesus monkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical methods were used to compare the localization of somatostatin (SRIF) in the human and rhesus monkey hypothalamus. The distribution of SRIF-containing cell bodies and fibers is similar in the two species. Perikarya are located predominantly in the periventricular region and to a lesser extent in the ventromedial nucleus. Fibers occur in dense clusters within the periventricular region, ventromedial nucleus, arcuate nucleus, median eminence, and pericommissural area of both species. Analysis of serial sections suggests that fibers originate from cells in the periventricular region, extend ventrally through the ventromedial and arcuate nuclei to terminate around the portal vessels of the infundibular stalk, and thereby participate in the regulation of anterior pituitary function. Somatostatinergic fibers are also found surrounding non-immunoreactive perikarya in the ventromedial nucleus and periventricular region of both primates. This arrangement may support somatostatin's postulated role as a neurotransmitter or neuromodulator. The strong similarity between the localization of hypothalamic SRIF in the human and rhesus monkey supports the use of the rhesus monkey as a model for the study of somatostatin as a neuroendocrine regulator in the human.
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    URL: http://dx.doi.org/10.1007/BF00222233
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  • 99
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    Vasopressin-immunoreactive cells in the dorsomedial hypothalamic region, medial amygdaloid nucleus and locus coeruleus of the rat (1983)
    Springer
    Cell & tissue research 233 (1983), S. 23-33 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Vasopressin ; Dorsomedial hypothalamus ; Amygdala ; Locus coeruleus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Recently, the existence of a vasopressin-immunoreactive cell group was described in the bed nucleus of the stria terminalis (van Leeuwen and Caffé 1983). In the present investigation additional nuclei containing vasopressin-immunoreactive cells were found, after colchicine pretreatment, in the dorsomedial hypothalamus, medial amygdaloid nucleus and the locus coeruleus. Vasopressin-immunoreactive cells in the dorsomedial hypothalamus and medial amygdaloid nucleus are small (8–14 μm and 10–14 μm, respectively), while those in the locus coeruleus are medium-sized (20–25 μm). Incubation with anti-bovine neurophysin II and anti-rat neurophysin revealed staining of the same cell group in the above-mentioned areas. None of these cell groups show stained cells after incubation with anti-oxytocin and anti-bovine neurophysin I. When sections of the homozygous Brattleboro rat, which shows a deficiency in vasopressin synthesis, are incubated with anti-vasopressin, anti-bovine neurophysin II, or anti-rat neurophysin, no immunoreactivity can be observed in these brain regions. The above-mentioned cell groups may contribute to the vasopressinergic innervation of brain sites that have been reported to persist after lesioning of the suprachiasmatic, paraventricular and bed nuclei of the stria terminalis.
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    URL: http://dx.doi.org/10.1007/BF00222229
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  • 100
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    Immunocytochemical identification of α-endorphin-like material in neurones of the brain and corpus cardiacum of the blowfly, Calliphora vomitoria (Diptera) (1983)
    Springer
    Cell & tissue research 233 (1983), S. 415-426 
    Details
    ISSN: 1432-0878
    Keywords: α-Endorphin ; Immunocytochemistry ; Median neurosecretory cells ; Peptidergic neurones ; Calliphora vomitoria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A group of the 24–26 paraldehyde fuchsin-positive median neurosecretory cells (MNC) in the pars intercerebralis of the brain of the blowfly, Calliphora vomitoria, has shown immunoreactivity towards three different antibodies to α-endorphin, a peptide that corresponds to the amino acid sequence present between residues 61 and 76 of the precursor molecule, β-lipotropin (β-LPH). The immunoreactive material could be followed in axons within the median bundle, the tract through which neurosecretory material from the MNC is passed down to the corpus cardiacum (CC). The α-endorphin-immunoreactive material was observed leaving the CC in the cardiac-recurrent nerve, dorsal to the proventriculus, in the direction of the abdomen. The cells that contain the α-endorphin-like material are different from those of the MNC that contain insulin-, pancreatic polypeptide-, and gastrin/CCK-like peptides. This finding demonstrates the considerable complexity and peptidergic nature of the MNC and constitutes further evidence that morphinomimetic-like peptides are present in the nervous system of invertebrates.
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    URL: http://dx.doi.org/10.1007/BF00238307
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