ALBERT

Description: No abstract available

Description: The research described in this paper is intended to support development and evaluation of preflight adaptation training (PAT) apparatus and procedures. Successful training depends on appropriate manipulation of visual and inertial stimuli that control perception of self-motion and self-orientation. For one part of this process, astronauts are trained to report their self-motion and self-orientation experiences. Before their space mission, they are exposed to the altered sensory environments produced by the PAT trainers. During and after the mission, they report their motion and orientation experiences. Subsequently, they are again exposed to the PAT trainers and are asked to describe relationships between their experiences in microgravity and following entry and their experiences in the trainers.

Description: Studies from space flights over the past two decades have demonstrated that there are basic physiological changes in humans during space flight. These changes include cephalad fluid shifts, loss of fluid and electrolytes, loss of muscle mass, space motion sickness, anemia, reduced immune response, and loss of calcium and mineralized bone. The cause of most of these manifestations is not known but the general approach has been to investigate systemic and hormonal changes. However, data from the 1973-1974 Skylabs, Spacelab 3 (SL-3), Spacelab D-I (SL-DI), and now the new SLS-1 missions support a more basic biological response to microgravity that may occur at the tissue, cellular, and molecular level. This report summarizes ground-based and SLS-1 experiments that examined the mechanism of loss of red blood cell mass in humans, the loss of bone mass and lowered osteoblast growth under space flight conditions, and loss of immune function in microgravity.

Description: A 65-kDa protein (called S1) from Spirochaeta bajacaliforniensis was identified as 'tubulin-like' because it cross-reacted with at least four different antisera raised against tubulin and was isolated, with a co-polymerizing 45-kDa protein, by warm-cold cycling procedures used to purify tubulin from mammalian brain. Furthermore, at least three genera of non-cultivable symbiotic spirochetes (Pillotina, Diplocalyx, and Hollandina) that contain conspicuous 24-nm cytoplasmic tubules displayed a strong fluorescence in situ when treated with polyclonal antisera raised against tubulin. Here we summarize results that lead to the conclusion that this 65-kDa protein has no homology to tubulin. S1 is an hsp65 stress protein homologue. Hsp65 is a highly immunogenic family of hsp60 proteins which includes the 65-kDa antigens of Mycobacterium tuberculosis (an active component of Freund's complete adjuvant), Borrelia, Treponema, Chlamydia, Legionella, and Salmonella. The hsp60s, also known as chaperonins, include E. coli GroEL, mitochondrial and chloroplast chaperonins, the pea aphid 'symbionin' and many other proteins involved in protein folding and the stress response.

Description: Bioreactor cell and tissue culture vessels can be used to study bone development in a simulated microgravity environment. These vessels will also provide an advantageous, low maintenance culture system on space station Freedom. Although many types of cells and tissues can potentially utilize this system, our particular interest is in developing bone tissue. We have characterized an organ culture system utilizing embryonic mouse pre-metatarsal mesenchyme, documenting morphogenesis and differentiation as cartilage rods are formed, with subsequent terminal chondrocyte differentiation to hypertrophied cells. Further development to form bone tissue is achieved by supplementation of the culture medium. Research using pre-metatarsal tissue, combined with the bioreactor culture hardware, could give insight into the advantages and/or disadvantages of conditions experienced in microgravity. Studies such as these have the potential to enhance understanding of bone development and adult bone physiology, and may help define the processes of bone demineralization experienced in space and in pathological conditions here on earth.

Description: A great deal of energy has been exerted over the years researching methods for regenerating and repairing bone and cartilage. Several techniques, especially bone implants and grafts, show great promise for providing a remedy for many skeletal disorders and chondrodystrophies. The bioreactor (rotating-wall vessel, RWV) is a cell culture system that creates a nurturing environment conducive to cell aggregation. Chondrocyte cultures have been studied as implants for repair and replacement of damaged and missing bone and cartilage since 1965 [Chesterman and Smith, J Bone Joint Surg 50B:184-197, 1965]. The ability to use large, tissue-like cartilage aggregates grown in the RWV would be of great clinical significance in treating skeletal disorders. In addition, the RWV may provide a superior method for studying chondrogenesis and chondrogenic mutations. Because the RWV is also reported to simulate many of the conditions of microgravity it is a very useful ground-based tool for studying how cell systems will react to microgravity.

Description: Exercise will be used primarily to countermeasure against deconditioning on extended space flight. In this paper we describe the development and evaluation of an expert system for exercise countermeasure protocol management. Currently, the system includes two major subsystems: baseline prescription and prescription adjustment. The baseline prescription subsystem is designed to provide initial exercise prescriptions while prescription adjustment subsystem is designed to modify the initial prescription based on the exercised progress. The system runs under three different environments: PC, SUN workstation, and Symbolic machine. The inference engine, baseline prescription module, prescription adjustment module and explanation module are developed under the Symbolic environment by using the ART (Automated Reasoning Tool) software. The Sun environment handles database management features and interfaces with PC environment to obtain physical and physiological data from exercise units on-board during the flight. Eight subjects' data have been used to evaluate the system performance by comparing the prescription of nine experienced exercise physiologists and the one prescribed by the expert system. The results of the validation test indicated that the performance of the expert system was acceptable.

Description: A leucine-requiring hybrid of Saccharomyces cerevisiae, homoallelic at the LEU1 locus (leu1-12/leu1-12) and heterozygous for three chromosome-VII genetic markers distal to the LEU1 locus, was employed to inquire: (1) whether spontaneous gene mutation and mitotic segregation of heterozygous markers occur in positive nonrandom association and (2) whether homozygous LEU1/LEU1 mutant diploids are generated. The results demonstrate that gene mutation of leu1-12 to LEU1 and mitotic segregation of heterozygous chromosome-VII markers occur in strong positive nonrandom association, suggesting that the stimulatory DNA lesion is both mutagenic and recombinogenic. In addition, genetic analysis of diploid Leu+ revertants revealed that approximately 3% of mutations of leu1-12 to LEU1 result in LEU1/LEU1 homozygotes. Red-white sectored Leu+ colonies exhibit genotypes that implicate post-replicational chromatid breakage and exchange near the site of leu1-12 reversion, chromosome loss, and subsequent restitution of diploidy, in the sequence of events leading to mutational homozygosis. By analogy, diploid cell populations can yield variants homozygous for novel recessive gene mutations at biologically significant rates. Mutational homozygosis may be relevant to both carcinogenesis and the evolution of asexual diploid organisms.

Description: The hypothesis derived from models of the multi-oligomeric chaperone complex suggests that partially denatured proteins bind in a central cavity in the aggregate. To test this hypothesis, the molecular chaperone, alpha crystallin, was bound to partially denatured forms of gamma crystallin, and the binding site was visualized by immunogold localization. In an alternative approach, gold particles were directly complexed with gamma crystallin, followed by binding to the alpha crystallin aggregate. In both cases, binding was localized to the central region of the aggregate, confirming for the first time that partially denatured proteins do indeed bind to a central region of the molecular chaperone aggregate.

Description: The plasma concentration of 1,25-dihydroxyvitamin D [1,25(OH)2D] decreases during skeletal unloading and increases when normal weight bearing is restored. To determine whether these changes in plasma 1,25(OH)2D reflect changes in production, metabolic clearance, or both we measured the kinetics of 1,25(OH)2D metabolism in rats whose skeletons were normally loaded, unloaded, or reloaded after a period of nonweight bearing. Skeletal unloading produced a transient but striking fall in the production (-73%) and plasma concentration (-72%) of 1,25(OH)2D without having a significant effect (〈 20%) on metabolic clearance. Skeletal reloading returned production to normal. Bone formation predictably decreased during unloading and returned to normal after return to weight bearing. No consistent changes in blood ionized calcium, plasma immunoreactive parathyroid hormone (irPTH), or plasma phosphorus occurred. These data suggest that the changes in plasma 1,25-(OH)2D associated with changes in skeletal weight bearing primarily reflect changes in 1,25(OH)2D production. The data provide no evidence that the changes in 1,25(OH)2D production are a consequence of changes in blood ionized calcium, plasma irPTH, or phosphorus.

Description: A three-dimensional model of the Tetrahymena thermophila group I intron is used to further explore the catalytic mechanism of the transphosphorylation reaction of the cleavage step. Based on the coordinates of the catalytic core model proposed by Michel and Westhof (Michel, F., Westhof, E. J. Mol. Biol. 216, 585-610 (1990)), we first converted their ligation step model into a model of the cleavage step by the substitution of several bases and the removal of helix P9. Next, an attempt to place a trigonal bipyramidal transition state model in the active site revealed that this modified model for the cleavage step could not accommodate the transition state due to insufficient space. A lowering of P1 helix relative to surrounding helices provided the additional space required. Simultaneously, it provided a better starting geometry to model the molecular contacts proposed by Pyle et al. (Pyle, A. M., Murphy, F. L., Cech, T. R. Nature 358, 123-128. (1992)), based on mutational studies involving the J8/7 segment. Two hydrated Mg2+ complexes were placed in the active site of the ribozyme model, using the crystal structure of the functionally similar Klenow fragment (Beese, L.S., Steitz, T.A. EMBO J. 10, 25-33 (1991)) as a guide. The presence of two metal ions in the active site of the intron differs from previous models, which incorporate one metal ion in the catalytic site to fulfill the postulated roles of Mg2+ in catalysis. The reaction profile is simulated based on a trigonal bipyramidal transition state, and the role of the hydrated Mg2+ complexes in catalysis is further explored using molecular orbital calculations.

Description: Atrial natriuretic peptide (ANP) is a fluid-regulating peptide hormone that promotes vasorelaxation, natriuresis, and diuresis. The mechanisms for the release of ANP and for its clearance from the circulation play important roles in modulating its biological effects. Recently, we have reported that the cell surface of an endothelial cell line, CPA47, could degrade 125I-ANP in the presence of EDTA. In this study, we have characterized this degradation of 125I-ANP. The kinetics of ANP degradation by the surface of CPA47 cells were first order, with a Km of 320 +/- 60 nM and Vmax of 35 +/- 14 pmol of ANP degraded/10 min/10(5) cells at pH 7.4. ANP is degraded by the surface of CPA47 cells over a broad pH range from 7.0-8.5. Potato carboxypeptidase inhibitor and bestatin inhibited 125I-ANP degradation, suggesting that this degradative activity on the surface of CPA47 cells has exopeptidase characteristics. The selectivity of CPA47 cell-surface degradation of ANP was demonstrated when 125I-ANP degradation was inhibited in the presence of neuropeptide Y and angiotensin I and II but not bradykinin, bombesin, endothelin-1, or substance P. The C-terminal amino acids phe26 and tyr28 were deduced to be important for ANP interaction with the cell-surface peptidase(s) based on comparison of the IC50 of various ANP analogues and other natriuretic peptides for the inhibition of ANP degradation. These data suggest that a newly characterized divalent cation-independent exopeptidase(s) that selectively recognizes ANP and some other vasoactive peptides exists on the surface of endothelial cells.

Description: The alpha crystallins are cytosolic proteins that co-localize and co-purify with actin-containing microfilaments. Affinity column chromatography employing both covalently-coupled actin or alpha crystallin was used to demonstrate specific and saturable binding of actin with alpha crystallin. This conclusion was confirmed by direct visualization of alpha aggregates bound to actin polymerized in vitro. The significance of this interaction in relation to the functional properties of these two polypeptides will be discussed.

Description: A variety of electrolytes (10-30 mol m-3) increased the relative growth rate of etiolated cucumber (Cucumis sativus L. cv. Burpee's Pickler) hypocotyls by 20-50% relative to water-only controls. The nonelectrolyte mannitol inhibited growth by 10%. All salts tested were effective, regardless of chemical composition or valence. Measurements of cell-sap osmolality ruled out an osmotic mechanism for the growth stimulation by electrolytes. This, and the nonspecificity of the response, indicate that an electrical property of the solutions was responsible for their growth-stimulating activity. Measurements of surface electrical potential supported this reasoning. Treatment with electrolytes also enhanced nutation and altered the pattern of phototropic curvature development. A novel analytical method for quantitating these effects on growth was developed. The evidence indicates that electrolytes influence an electrophysiological parameter that is involved in the control of cell expansion and the coordination of growth underlying tropisms and nutations.

Description: No abstract available

Description: Immunoreactive neuropeptide Y and dynorphin have been localized in the brain and pituitary gland of the platyfish, Xiphophorus maculatus, at different ages and stages of development from birth to sexual maturity. Immunoreactive neuropeptide Y was found in perikarya and tracts of the nucleus olfactoretinalis, telencephalon, ventral tegmentum and in the neurohypophysis and in the three regions of the adenohypophysis. Immunoreactive dynorphin was found in nerve tracts in the olfactory bulb and in cells of the pars intermedia and the rostral pars distalis of the pituitary gland.

Description: The purpose of this study was to determine if fluid-electrolyte, renal, hormonal, and cardiovascular responses during and after multi-hour water immersion were associated with aerobic training. Additionally, we compared these responses in those who trained in a hypogravic versus a 1-g environment. Seventeen men comprised three similarly aged groups: six long-distance runners, five competitive swimmers, and six untrained control subjects. Each subject underwent 5 h of immersion in water [mean (SE)] 36.0 (0.5) degrees C to the neck. Immediately before and at each hour of immersion, blood and urine samples were collected and analyzed for sodium (Na), potassium, osmolality, and creatinine (Cr). Plasma antidiuretic hormone and aldosterone were also measured. Hematocrits were used to calculate relative changes in plasma volume (% delta Vpl). Heart rate response to submaximal cycle ergometer exercise (35% peak oxygen uptake) was measured before and after water immersion. Water immersion induced significant increases in urine flow, Na clearance (CNa), and a 3-5% decrease in Vpl. Urine flow during immersion was greater (P 〈 0.05) in runners [2.4 (0.4) ml.min-1] compared to controls [1.3 (0.1) ml.min-1]. However, % delta Vpl, CCr, CNa and CH2O during immersion were not different (P 〉 0.05) between runners, swimmers, and controls. After 5 h of immersion, there was an increase (P 〈 0.05) in submaximal exercise heart rate of 9 (3) and 10 (3) beats.min-1 in both runners and controls, respectively, but no change (P 〉 0.05) was observed in swimmers.(ABSTRACT TRUNCATED AT 250 WORDS).

Description: On a partition chromatographic column in which the support is Kieselguhr and the stationary phase is sulfuric acid solution (2 mol/L), three components of compound theophylline tablet were simultaneously eluted by chloroform and three other components were simultaneously eluted by ammonia-saturated chloroform. The two mixtures were determined by computer-aided convolution curve method separately. The corresponding average recovery and relative standard deviation of the six components were as follows: 101.6, 1.46% for caffeine; 99.7, 0.10% for phenacetin; 100.9, 1.31% for phenobarbitone; 100.2, 0.81% for theophylline; 99.9, 0.81% for theobromine and 100.8, 0.48% for aminopyrine.

Description: No abstract available

Description: In tissues such as bone marrow with normally high rates of cell division, proliferation is tightly coordinated with cell differentiation. Survival, proliferation and differentiation of early hematopoietic progenitor cells depend on the growth factors, interleukin 3 (IL-3) and/or granulocyte-macrophage colony stimulating factor (GM-CSF) and their synergism with other cytokines. We provide evidence that a characteristic shared by a diverse group of compounds with demonstrated leukemogenic potential is the ability to act synergistically with GM-CSF. This results in an increase in recruitment of a resting population of hematopoietic progenitor cells normally unresponsive to the cytokine and a twofold increase in the size of the proliferating cell population normally regarded to be at risk of transformation in leukemogenesis. These findings support the possibility that transient alterations in hematopoietic progenitor cell differentiation may be an important factor in the early stages of development of leukemia secondary to chemical or drug exposure.

Description: In fertilized eggs of the frog Xenopus, the vegetal yolk mass rotates away from the future dorsal side (J. P. Vincent and J. Gerhart, 1987, Dev. Biol. 123, 526-539), and a major rearrangement of the deep animal hemisphere cytoplasm produces a characteristic swirl in the prospective dorsal side (M. V. Danilchik and J. M. Denegre, 1991, Development 111, 845-856). The relationship between this swirl and determination of the dorsal-ventral axis was further investigated by attempting to experimentally separate the positions of the swirl and the dorsal-ventral axis. Eggs were obliquely oriented in the gravity field to respecify the direction of yolk mass rotation and the position of the dorsal-ventral axis. When yolk mass rotation occurred in the absence of a sperm, as in activated eggs, a swirl pattern formed on the side away from which the yolk mass had rotated. In fertilized eggs tipped with the sperm entry point (SEP) down or to the side, swirl patterns were always found to form on the side away from which the yolk mass was displaced. However, in eggs tipped SEP up, in which the yolk mass was forced to rotate away from the SEP, more complicated rearrangements were observed in addition to the rotation-oriented swirl. Because the direction of yolk mass rotation was found to be influenced by both gravity and the actual position of the SEP in obliquely oriented eggs (SEP to the side), such complicated rearrangement patterns may result from opposing forces generated by both yolk mass rotation and the expanding sperm aster. Thus, except in cases in which the influences of SEP position and unit gravity opposed each other, it was not possible to experimentally separate the position of the deep cytoplasmic swirl from the direction of yolk mass rotation, and therefore the position of the prospective dorsal side.

Description: This study ascertained the effects of 9 days of zero gravity on the relative (percentage of total) and calculated absolute (mg/muscle) content of isomyosin expressed in both antigravity and locomotor skeletal muscle of ground control (CON) and flight-exposed (FL) rats. Results showed that although there were no differences in body weight between FL and CON animals, a significant reduction in muscle mass occurred in the vastus intermedius (VI) (P 〈 0.05) but not in the vastus lateralis (VL) or the tibialis anterior. Both total muscle protein and myofibril protein content were not different between the muscle regions examined in the FL and CON groups. In the VI, there were trends for reductions in the relative content of type I and IIa myosin heavy chains (MHCs) that were offset by increases in the relative content of both type IIb and possibly type IIx MHC protein (P 〉 0.05). mRNA levels were consistent with this pattern (P 〈 0.05). The same pattern held true for the red region of the VL as examined at both the protein and mRNA level (P 〈 0.05). When the atrophy process was examined, there were net reductions in the absolute content of both type I and IIa MHCs that were offset by calculated increases in type IIb MHC in both VI and red VL. Collectively, these findings suggest that there are both absolute and relative changes occurring in MHC expression in the "red" regions of antigravity skeletal muscle during exposure to zero gravity that could affect muscle function.

Description: The benefits of using a hypersonic waverider for spacecraft trajectory modification are presented. A waverider is a hypersonic vehicle specifically designed so that the undersurface bow shock is attached to the leading edge, which provides for the highest known lift-to-drag ratios achievable at high Mach number flight. Several viable space missions are suggested which could use such configurations for low-drag aero-assisted maneuvers in planetary atmospheres. It is shown that large changes in the spacecraft velocity vector can be accomplished with acceptably small losses in energy due to drag using a waverider aeroshell. The primary advantage of an aero-assist maneuver is suggested by comparison to a traditional gravity-assist trajectory. Some scaling laws are presented for comparing waveriders designed for different planetary atmospheres, and it is shown that the compositional differences between the terrestrial planets has a minimal impact on waverider design.

Description: The severity and significance for the future use of space of the continued deposition of orbital debris into the near-earth environment is examined, and approaches to controlling the growing orbital debris population are discussed. Three categories of options are considered: those requiring minimal impact on operations, those requiring changes in hardware or operations, and those requiring technology development. The necessary role of the international community is these efforts is emphasized.

Description: The scientific objective of the NASA Small-class Explorer Mission SAMPEX is summarized. A brief history of the Small Explorer program is provided along with a description of the SAMPEX project development and structure. The spacecraft and scientific instrument configuration is presented. The orbit of SAMPEX has an altitude of 520 by 670 km and an 82 deg inclination. Maximum possible power is provided by articulated solar arrays that point continuously toward the sun. Highly sensitive science instruments point generally toward the local zenith, especially over the terrestrial poles, in order to measure optimally the galactic and solar cosmic ray flux. Energetic magnetospheric particle precipitation is monitored at lower geomagnetic latitudes. The spacecraft uses several innovative approaches including an optical fiber bus, powerful onboard computers, and large solid state memories (instead of tape recorders). Spacecraft communication and data acquisition are discussed, and the space- and ground-segment data flows are summarized. A mission lifetime of 3 years is sought with the goal of extending data acquisition over an even longer portion of the 11-year solar activity cycle.

Description: Unsteady flow behavior and load characteristics of a 2D VR-7 airfoil with and without a leading-edge slat were studied in the water tunnel of the Aeroflightdynamics Directorate, NASA Ames Research Center. Both airfoils were oscillated sinusoidally between 5 and 25 deg at Re = 200,000 to obtain the unsteady lift, drag, and pitching moment data. A fluorescent dye was released from an orifice located at the leading edge of the airfoil for the purpose of visualizing the boundary layer and wake flow. The flowfield and load predictions of an incompressible Navier-Stokes code based on a velocity-vorticity formulation were compared with the test data. The test and predictions both confirm that the slatted VR-7 airfoil delays both static and dynamic stall as compared to the VR-7 airfoil alone.

Description: Part of the objective of the cooled infrared telescope on Spacelab 2 was to determine the particle environment around the Space Shuttle. The telescope scanned the sky in six wavelength bands ranging from 2 to 100 microns with high time resolution. Dust particles could be identified from their particular signature in the data stream. Particle data from about 4 h early in the mission were analyzed in terms of size, color temperature, velocity, and time. The 1100 particles that were seen were slow moving and ranged in color temperature from 190 to 350 K with a few much hotter. The minimum detectable diameter is between 5 and 13 microns, depending on temperature. The size distribution resembles sample distributions collected at a Shuttle preparation facility. Although particle detection rates varied widely with time, no specific events were identified to be associated with particle production. It was not possible to determine the particle composition, although it was probably not ice.

Description: Analytic expressions have been derived for estimating the nonablating laminar and turbulent boundary-layer convective heating rates on inclined flat surfaces for the Martian atmosphere in thermochemical equilibrium. The equations are valid in the speed and altitude regime where aerobraking would occur at Mars. Comparisons with limited experimental measurements and calculations for CO2 (the Martian atmosphere is 95.6 percent CO2) yielded reasonably good agreement, especially for the ratios of heating rates in CO2 to those in air at the same conditions. In the aerobraking speed regime, the laminar flat surface boundary layer heating rates are 15-25 percent greater at Mars than in air. The differences between the turbulent heating rates are even more pronounced. The turbulent heating rates can be over 50 percent greater at Mars than in air at the same flight conditions.

Description: The ways of plausibly evaluating the scientific payoff of prospective mission architectures within the administrative framework of NASA's Space Exploration Initiative (SEI) are discussed. Attention is given to the expanding goals of a modestly implemented unmanned exploration focus, an aggressive implementation of that focus, and an equally aggressive implementation of a manned exploration priority, relative to the Apollo moon-landing program of the 1960s-1970s.

Description: The general purpose experiment facility flown aboard Space Shuttle USML-1 and known as the Glovebox is briefly discussed. Glovebox enabled scientists to perform materials science, fluids, and combustion experiments safely without contaminating the closed environment of Spacelab and endangering the crew. The evolution of Glovebox, its special features, and its hardware are described. The Glovebox experiments are summarized along with postmission and crew debriefing. Future uses of Glovebox are discussed.

Description: An incompressible three-dimensional laminar boundary-layer flow over a swept wing is used as a model to study both the wall-curvature and streamline-curvature effects on the stationary crossflow instability. The basic state is obtained by solving the full Navier-Stokes (N-S) equations numerically. The linear disturbance equations are cast on a fixed, body-intrinsic, curvilinear coordinate system. Those nonparallel terms which contribute mainly to the streamline-curvature effect are retained in the formulation of the disturbance equations and approximated by their local finite difference values. The resulting eigenvalue problem is solved by a Chebyshev collocation method. The present results indicate that the convex wall curvature has a stabilizing effect, whereas the streamline curvature has a destabilizing effect. A validation of these effects with an N-S solution for the linear disturbance flow is provided.

Description: The paper presents a general approach to constructing mean velocity profiles for compressible turbulent boundary layers with isothermal or adiabatic walls. The theory is based on a density-weighted transformation that allows the extension of the incompressible similarity laws of the wall to the compressible regions. The velocity profile family is compared to a range of experimental data, and excellent agreement is obtained. A self-consistent skin friction law, which satisfies the proposed velocity profile family, is derived and compared with the well-known Van Driest II theory for boundary layers in zero pressure gradient. The results are found to be at least as good as those obtained by using the Van Driest II transformation.

Description: A number of chemical-kinetic problems related to phenomena occurring behind a shock wave surrounding an object flying in the earth atmosphere are discussed, including the nonequilibrium thermochemical relaxation phenomena occurring behind a shock wave surrounding the flying object, problems related to aerobraking maneuver, the radiation phenomena for shock velocities of up to 12 km/sec, and the determination of rate coefficients for ionization reactions and associated electron-impact ionization reactions. Results of experiments are presented in form of graphs and tables, giving data on the reaction rate coefficients for air, the ionization distances, thermodynamic properties behind a shock wave, radiative heat flux calculations, Damkoehler numbers for the ablation-product layer, together with conclusions.

Description: The development of implicit upwind algorithms for the solution of the three-dimensional, time-dependent Euler equations on unstructured tetrahedral meshes is described. The implicit temporal discretization involves either a two-sweep Gauss-Seide relaxation procedure, a two-sweep Point-Jacobi relaxation procedure, or a single-sweep Point-Implicit procedure; the upwind spatial discretization is based on the flux-difference splitting of Roe. Detailed descriptions of the three implicit solution algorithms are given, and calculations for the Boeing 747 transport configuration are presented to demonstrate the algorithms. Advantages and disadvantages of the implicit algorithms are discussed. A steady-state solution for the 747 configuration, obtained at transonic flow conditions using a mesh of over 100,000 cells, required less than one hour of CPU time on a Cray-2 computer, thus demonstrating the speed and robustness of the general capability.

Description: Two-dimensional CFD analyses are presented related to the ground testing of hypersonic, air-breathing models which feature scramjet exhaust flow simulation. CFD analysis indicates that it is possible to test aftbody powered hypersonic airbreather configurations in a static, pumped-down environment to obtain aftbody aerodynamic performance data.

Description: Experimental studies have been conducted to assess Reynolds and Mach number effects on a supercritical multielement airfoil. The airfoil is representative of the stall-critical station of an advanced transport wing design. The experimental work was conducted as part of a cooperative program between the Douglas Aircraft Company and the NASA Langley Research Center to improve current knowledge of high-lift flows and to develop a validation data base with practical geometries/conditions for emerging computational methods. This article describes results obtained for both landing and takeoff multielement airfoils (four- and three-element configurations) for a variety of Mach/Reynolds number combinations up to flight conditions. Effects on maximum lift are considered for the landing configurations, and effects on both lift and drag are reported for the takeoff geometry. The present test results revealed considerable maximum lift effects on the three-element landing configuration for Reynolds number variations, and significant Mach number effects on the four-element airfoil.

Description: This paper presents an alternate approach for the generation of volumetric grids for supersonic and hypersonic flows about complex configurations. The method uses parametric two-dimensional block-face grid definitions, within the framework of GRIDGEN2D. The incorporation of a face decomposition reduces complex surfaces to simple shapes. These simple shapes are recombined to obtain the final face definition. The advantages of this method include the reduction of overall grid generation time through the use of vectorized computer code, the elimination of generating matching block faces, and simplified boundary conditions.

Description: An algorithm is described that calculates inviscid, laminar, and turbulent flows on triangular meshes with an upwind discretization. A brief description of the base solver and the multigrid implementation is given, followed by results that consist mainly of convergence rates for inviscid and viscous flows over a NACA four-digit airfoil section. The results show that multigrid does accelerate convergence when the same relaxation parameters that yield good single-grid performance are used; however, larger gains in performance can be realized by doing less work in the relaxation scheme.

Description: An evaluation is presented of the consequences of NASA's study of the ways in which the launch of a Space Shuttle generates a plume of HCl and Al2O3; in combination with water from the Shuttle's sound-suppressor cooling water system, these substances can create a corrosive and hard-to-remove Al2O3 coating on nearby surfaces. Attention is given to the site-specific character of such environmental complications, the need to involve environmental-risk specialists in hardware-development teams, and the nonlinearity of many environmental impacts.

Description: The interaction of a turbulent boundary layer with rarefaction and shock waves in flows past inclined steps was investigated experimentally and theoretically. The experiments were carried out in supersonic wind tunnels with test sections of 0.6 x 0.6 m and 0.2 x 0.2 m, with adiabatic conditions on the surface model. Based on detailed measurements of pressure fields, velocity, and surface friction, combined with results of optical visualization and analysis of limiting flow lines, three characteristic flow regimes are identified. These are (1) nonseparated flow at small step angles, (2) formation of a local separation zone with a free separation point at moderate step angles, and (3) formation of a large-scale separated flow with a fixed separation point at sufficiently large step angles. The quantitative gasdynamic schemes and surface pressure distributions for these flow regimes are shown for Mach 2.85.

Description: An account is given of experimental and computational results on the mixing of single, double, and opposed rows of jets characterized by an either isothermal or variable temperature mainstream in a confined subsonic crossflow; these flow configurations are typical of gas turbine combustor dilution chambers. It is established that the momentum-flux ratio is the most significant flow variable. Combinations of flow and geometry yielding optimum mixing were identified from experimental and computational results. The orifice spacing and momentum-flux relationships affected jet structure, which was significantly different between jets injected from the inner wall of a turn and those injected from the outer wall.

Description: The Space Shuttle Orbiter Endeavour undertook its maiden mission (STS-49) on May 7, 1992, exactly one year after its arrival at the Kennedy Space Center, in order to attach a perigee 'kick-motor' to the Intelsat-VI communications satellite which would carry it from the LEO where it was marooned to GEO. Endeavour's secondary payload was the Assembly of Space Station by Extravehicular Methods experiment. A log is here presented of STS-49.

Description: The essential elements of a computational fluid dynamics analysis of the HL-20/personnel launch system aerothermal environment at hypersonic speeds including surface definition, grid generation, solution techniques, and visual representation of results are presented. Examples of solution technique validation through comparison with data from ground-based facilities are presented, along with results from computations at flight conditions. Computations at flight points indicate that real-gas effects have little or no effect on vehicle aerodynamics and, at these conditions, results from approximate techniques for determining surface heating are comparable with those obtained from Navier-Stokes solutions.

Description: Definition of the aerothermal environment is critical to any vehicle such as the HL-20 Personnel Launch System that operates within the hypersonic flight regime. Selection of an appropriate thermal protection system design is highly dependent on the accuracy of the heating-environment prediction. It is demonstrated that the entry environment determines the thermal protection system design for this vehicle. The methods used to predict the thermal environment for the HL-20 Personnel Launch System vehicle are described. Comparisons of the engineering solutions with computational fluid dynamic predictions, as well as wind-tunnel test results, show good agreement. The aeroheating predictions over several critical regions of the vehicle, including the stagnation areas of the nose and leading edges, windward centerline and wing surfaces, and leeward surfaces, are discussed. Results of predictions based on the engineering methods found within the MINIVER aerodynamic heating code are used in conjunction with the results of the extensive wind-tunnel tests on this configuration to define a flight thermal environment. Finally, the selection of the thermal protection system based on these predictions and current technology is described.

Description: Aerobraking Magellan would provide the possibility of obtaining gravity field data for Venus all the way to the poles. Attempts to accomplish aerobraking, which began on May 25, 1993 in the Magellan Transition Experiment, are discussed.

Description: Experiments were conducted to observe the cross-sectional structure and streamwise growth of round transverse liquid jets injected into a highly accelerated boundary layer in supersonic flow. The accompanying shock structure was also visualized. In one case, a round jet of acetone was injected into a fully turbulent Mach 2.5 boundary layer that was subsequently accelerated and partially laminarized through a sharp Prandtl-Meyer expansion corner. In the second case, a jet was injected into the laminarized Mach 3.2 boundary layer downstream of the expansion corner at the same jet-to-freestream momentum ratio. The jet and shock structure in both cases were visualized using schlieren optics. Wall-flow patterns were visualized using paints. It was found that the lateral spreading of jets injected downstream of the expansion fan was augmented close to the wall and had a cross-sectional structure significantly different from that of the jet injected upstream: the upstream jet spreads rapidly at the expansion corner in both the lateral and vertical directions.

Description: Computational results are presented to illustrate the powered aftbody effects of representing the scramjet inlet on a generic hypersonic vehicle with a fairing, to divert the external flow, as compared to an operating flow-through scramjet inlet. This study is pertinent to the ground testing of hypersonic, airbreathing models employing scramjet exhaust flow simulation in typical small-scale hypersonic wind tunnels. The comparison of aftbody effects due to inlet representation is well-suited for computational study, since small model size typically precludes the ability to ingest flow into the inlet and perform exhaust simulation at the same time. Two-dimensional analysis indicates that, although flowfield differences exist for the two types of inlet representations, little, if any, difference in surface aftbody characteristics is caused by fairing over the inlet.

Description: The Voyager mission based on two spacecraft, Voyager 1 and Voyager 2, launched on September 5, 1977 and August 20 1977, respectively, is reviewed. The mission was designed to take advantage of a rare geometric arrangement of Jupiter, Saturn, Uranus, and Neptune that occurs only once every 176 years. Particular attention is given to data on Jupiter's ring and moons, the discovery of active volcanoes on Io, Jovian magnetism, Saturn's atmosphere, Titan and other moons, Uranus and Miranda, Uranian moons and rings, Triton's atmosphere.

Description: The launching of Discovery on the STS-56 mission in April 1993 is described. The installation of experiments and launch delays of the space shuttle are discussed. A review of the activities of the crew is given for each day of the mission.

Description: In a tethered-satellite system, two satellites travelling in different orbits are forced to circle the earth in the same time period. The lower satellite is dragged by the tether to a higher orbital speed, while the upper one tends to move higher. This generates a tension which maintains the system in a stable configuration; the tether is aligned with a radius projecting outward from the earth's center. Such a system has been demonstrated by the TSS-1 tethered satellite carried by the Space Shuttle's STS-46 mission. The dynamic and the electrodynamic behavior of the system at long tether lengths were not, however, evaluated due to system malfunctions.

Description: A detailed description of the bow-shock behavior associated with a conical-walled cavity with a flat circular base at M(infinity) = 10 is presented. An experimental test was performed on this configuration, and measurements of shock-oscillation frequency and amplitude, as well as shock shape, were recorded by a number of techniques. A laser-interferometer system was used for the first time during this test to determine bow-shock oscillation frequency in a nonintrusive manner. The primary behavior was a stable, periodically oscillating bow shock. Attention is also given to a violent bow-shock instability.

Description: Hypersonic reactive flowfield results are presented for the flow about an axisymmetric representation of a three-dimensional aeroassisted vehicle using the viscous shock-layer (VSL) and Navier-Stokes (NS) equations for low- and high-altitude entry conditions, respectively. Solutions are limited to the stagnation region. The NS results have been obtained by employing the surface recombination boundary condition for an 11-species air model, whereas the VSL calculations are carried out by using a 5-species model. Three widely used surface recombination rate models are implemented with these two flowfield calculation procedures. The low-density (or high altitude) NS solutions are obtained with surface slip equations and compare quite well with the direct simulation Monte Carlo predictions. For higher densities (or lower altitudes), the no-slip VSL solutions are considered adequate. Results from this study highlight the effect of surface catalyticity on surface heating and electron number density.

Description: Qualitative features of the inviscid instability characteristics of hypersonic boundary-layer flows over a flat plate are considered. The instability of a viscous hypersonic boundary layer which exists far downstream from the leading edge of the plate. It is shown that the vorticity mode of instability operates on a different lengthscale from that obtained using a Chapman viscosity law. The growth rate predicted by a linear viscosity law is found to overestimate the size of the growth rate. The inviscid instability of the boundary layer near the leading edge interaction zone is discussed focusing on the strong-interaction zone which occurs sufficiently close to the leading edge. The vorticity mode in this regime is found to be unstable.

Description: The processing and course of the STS-46 Space Shuttle Atlantis mission are described. Problems experienced by the astronaut team in deploying the Tethered Satellite System during the mission are recounted.

Description: A closed-form analysis of flow in a two-dimensional subsonic wind tunnel that uses sidewall suction around the model to reduce sidewall boundary-layer effects is presented. The model problem that is treated involves a flat plate airfoil in a tunnel with a suction window shaped to permit an analytic solution. This solution shows that the lift coefficient depends explicitly on the porosity parameter of the suction window and implicitly on the suction pressure differential. For a given sidewall displacement thickness, the lift coefficient increases as the suction-window porosity decreases.

Description: The Abdominal gene is a member of the single homeotic complex of the beetle, Tribolium castaneum. An integrated developmental genetic and molecular analysis shows that Abdominal is homologous to the abdominal-A gene of the bithorax complex of Drosophila. abdominal-A mutant embryos display strong homeotic transformations of the anterior abdomen (parasegments 7-9) to PS6, whereas developmental commitments in the posterior abdomen depend primarily on Abdominal-B. In beetle embryos lacking Abdominal function, parasegments throughout the abdomen are transformed to PS6. This observation demonstrates the general functional significance of parasegmental expression among insects and shows that the control of determinative decisions in the posterior abdomen by homeotic selector genes has undergone considerable evolutionary modification.

Description: In one approach to modeling brain function, sensorimotor integration is described as geometrical mapping among coordinates of non-orthogonal frames that are intrinsic to the system; in such a case sensors represent (covariant) afferents and motor effectors represent (contravariant) motor efferents. The neuronal networks that perform such a function are viewed as general tensor transformations among different expressions and metric tensors determining the geometry of neural functional spaces. Although the non-orthogonality of a coordinate system does not impose a specific geometry on the space, this "Tensor Network Theory of brain function" allows for the possibility that the geometry is non-Euclidean. It is suggested that investigation of the non-Euclidean nature of the geometry is the key to understanding brain function and to interpreting neuronal network function. This paper outlines three contemporary applications of such a theoretical modeling approach. The first is the analysis and interpretation of multi-electrode recordings. The internal geometries of neural networks controlling external behavior of the skeletomuscle system is experimentally determinable using such multi-unit recordings. The second application of this geometrical approach to brain theory is modeling the control of posture and movement. A preliminary simulation study has been conducted with the aim of understanding the control of balance in a standing human. The model appears to unify postural control strategies that have previously been considered to be independent of each other. Third, this paper emphasizes the importance of the geometrical approach for the design and fabrication of neurocomputers that could be used in functional neuromuscular stimulation (FNS) for replacing lost motor control.

Description: We have identified three distinct cell phenotypes with respect to the conditions under which cells became susceptible to TNF-mediated lysis. These conditions include: 1) treatment with the protein synthesis inhibitor, cycloheximide; 2) contact with activated macrophages, and 3) infection with vaccinia virus. Whereas vaccinia virus-infected 3T3 cells became sensitive to soluble TNF, F5b cells required contact with activated macrophages. We showed that the "macrophage-resistant" F5m cells did not become sensitive to TNF or to killing by activated macrophages after infection with vaccinia virus. Therefore, vaccinia infection does not sensitize all cells to TNF. We also determined the pathways of lysis for cells after sensitization. Whereas 3T3, LM929, and F5b cells were killed by the process of necrosis, F5m cells lysis was characterized by the release of low mol wt DNA fragments (apoptosis).

Description: This study extends to the protein level our previous observations, which had established the stage and cellular specificity of expression of hsp86 and hsp84 in the murine testis in the absence of exogenous stress. Immunoblot analysis was used to demonstrate that HSP86 protein was present throughout testicular development and that its levels increased with the appearance of differentiating germ cells. HSP86 was most abundant in the germ cell population and was present at significantly lower levels in the somatic cells. By contrast, the HSP84 protein was detected in the somatic cells of the testis rather than in germ cells. The steady-state levels of HSP86 and HSP84 paralleled the pattern of the expression of their respective mRNAs, suggesting that regulation at the level of translation was not a major mechanism controlling hsp90 gene expression in testicular cells. Immunoprecipitation analysis revealed that a 70-kDa protein coprecipitated with the HSP86/HSP84 proteins in testicular homogenates. This protein was identified as an HSP70 family member by immunoblot analysis, suggesting that HSP70 and HSP90 family members interact in testicular cells.

Description: Depression of lymphocyte response to mitogens in cosmonauts after space flight was reported for the first time in the early 1970s by Soviet immunologists. Today we know that depression of lymphocyte function affects at least 50% of space crew members. Investigations on the ground on subjects undergoing physical and psychological stress indicate that stress is a major factor in immune depression of astronauts. This is despite the fact that weightlessness per se has a strong inhibitory effect on lymphocyte activation in vitro. Although the changes observed never harmed the health of astronauts, immunological changes must be seriously investigated and understood in view of long-duration flight on space stations in an Earth orbit, to other planets such as Mars and to the Moon.

Description: The Tetrahymena group I ribozyme catalyses a sequence-specific phosphodiester cleavage reaction on an external RNA oligonucleotide substrate in the presence of a divalent metal cation cofactor. This reaction proceeds readily with either Mg2+ or Mn2+, but no detectable reaction has been reported when other divalent cations are used as the sole cofactor. Cations such as Ca2+, Sr2+ and Ba2+ can stabilize the correct folded conformation of the ribozyme, thereby partially alleviating the Mg2+ or Mn2+ requirement. But catalysis by the ribozyme involves coordination of either Mg2+ or Mn2+ at the active site, resulting in an overall requirement for one of these two cations. Here we use an in vitro evolution process to obtain variants of the Tetrahymena ribozyme that are capable of cleaving an RNA substrate in reaction mixtures containing Ca2+ as the divalent cation. These findings extend the range of different chemical environments available to RNA enzymes and illustrate the power of in vitro evolution in generating macromolecular catalysts with desired properties.