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  • starch composition  (5)
  • somatic embryogenesis  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Revertants of the amylose-free potato clone 86.040 (2n = 1x = 12)
    Amsterdam : Elsevier
    Plant Science 67 (1990), S. 177-182 
    Details
    ISSN: 0168-9452
    Keywords: granule bound starch synthase ; mutation ; potato ; reversion ; starch composition
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90241-F
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  • 2
    Electronic Resource
    Electronic Resource
    Development of a cyclic somatic embryogenesis regeneration system for leek (Allium ampeloprasum L.) using zygotic embryos (1995)
    Springer
    Plant cell reports 14 (1995), S. 227-231 
    Details
    ISSN: 1432-203X
    Keywords: leek ; Allium ampeloprasum L. ; somatic embryogenesis ; genotypic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten families were investigated for somatic embryogenesis. There was a genetic relationship with respect to somatic embryo production between the reciprocal crosses. From each family one genotype was selected for investigating cyclic somatic embryogenesis. Different levels of somatic embryo production were found between the expiants of zygotic and somatic embryos. The two best genotypes, 92.001-03 and 92.002-33 produced twice as many somatic embryos as the overall average. On average, 56% of the somatic embryos finally developed into greenhouse plantlets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233638
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  • 3
    Electronic Resource
    Electronic Resource
    Phenotypic and genotypic characterization of an amylose-free starch mutant of the potato (1989)
    Springer
    Euphytica 44 (1989), S. 43-48 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; mutant ; starch composition ; amylose-free ; embryo rescue ; inheritance ; phenotype ; breeding ; karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amylose-free (amf) potato mutant 86.040 has been characterized phenotypically and genotypically. Not only storage starch in tubers and metabolic starch in leaves but also starch in cells with specific functions, such as columella cells in the root cap and guard cells of stomata, was amylose-free. Doubled amf-clones of 86.040 flowered well, but the percentage of stainable pollen was low (8.2%). Therefore, they were used as female parent in crosses with diploid amylose-containing plants. Over 400 pollinations gave 13 berries, all dropping off prematurely; they contained 78 ovules with embryos at different developmental stages. Twenty F1 plants were obtained by embryo rescue, all having amylose starch. Three of them were characterized more extensively. They flowered well and pollen stainabilitity as well as seed set were high. Analysis of the F1 plants and of F1 × F1 progeny plants showed that the amf-character is monogenic and recessive. The locus involved was designated amf +and the mutated allele amf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022597
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  • 4
    Electronic Resource
    Electronic Resource
    Plant regeneration via organogenesis and somatic embryogenesis in callus cultures derived from mature zygotic embryos of leek (Allium ampeloprasum L.) (1996)
    Springer
    Euphytica 91 (1996), S. 261-270 
    Details
    ISSN: 1573-5060
    Keywords: Allium ampeloprasum ; 2,4-dichlorophenoxy acetic acid ; Murashige and Skoog medium ; organogenesis ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A high frequency plant regeneration system via organogenesis and somatic embryogenesis was established with callus cultures derived from mature zygotic embryos of different leek genotypes (Allium ampeloprasum L.). Four different callus types with varying morphogenetic potential were obtained. Relatively high concentrations of the auxin 2,4-dichlorophenoxy-acetic acid reduced callus weight and subsequent shoot regeneration and primordia formation of the callus. Shoot regeneration and primordia formation of the callus decreased after prolonged subculture on media containing 2,4-dichlorophenoxy acetic acid. A callus growth period of six weeks on Murashige and Skoog medium with 0.25–0.5 mg l-1 2,4-dichlorophenoxy acetic acid showed the highest rate of shoot regeneration after transfer of callus to regeneration medium with 1 mg l-1 kinetin. Differences between leek genotypes in callus type, callus weight, shoot regeneration and primordia formation were observed. Histological observations showed that plant regeneration took place, both via the pathway of somatic embryogenesis and organogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033086
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  • 5
    Electronic Resource
    Electronic Resource
    Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato (1991)
    Springer
    Euphytica 59 (1991), S. 83-91 
    Details
    ISSN: 1573-5060
    Keywords: Agrobacterium rhizogenes ; antisense RNA ; granule-bound starch synthase ; Solanum tuberosum ; starch composition ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025364
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  • 6
    Electronic Resource
    Electronic Resource
    Cytological studies on adventitious shoots and minitubers of a monoploid potato clone (1988)
    Springer
    Euphytica 39 (1988), S. 213-219 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; adventitious shoot induction ; minituber induction ; starch composition ; cytology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A three step procedure for adventitious shoot regeneration on leaf explants of monoploid potato clone H7322 and a minituber induction procedure on stem segments have been described. Chromosome counts on 92 adventitious shoots showed that 85% of them had been polyploidized, i.e., 71% were diploid, 1% tetraploid, and 13% were mixoploid. Cytophotometric studies on nuclei of soil grown tubers of tetraploid cv Astarte, of 1x, 2x and 4x adventitious shoots of H7322, and of diploid H2578 showed in all cases polyploidization with prominent classes up to 8C and 16C. However, nuclei of pith cells of 5 weeks old minitubers which had developed on monoploid H7322 itself or on 1x adventitious shoots of H7322 showed predominantly 1C and 2C values. Pith cells of minitubers of monoploid H7322 were screened, after iodine staining, for the presence of variant cells containing reddish-brown staining (amylose-free) starch. In more than 75% of the investigated minitubers one or a few of such variant cells were found indicating that such a variation occurs in minitubers of monoploid potato and that this variant character is expressed in cells of vegetative storage organs like potato tubers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037098
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  • 7
    Electronic Resource
    Electronic Resource
    Regeneration and transformation of cassava (1997)
    Springer
    Euphytica 96 (1997), S. 153-161 
    Details
    ISSN: 1573-5060
    Keywords: cassava ; transformation ; review ; somatic embryogenesis ; adventitious shoot formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A prerequisite for the development of a successful transformation system is the availability of efficient regeneration systems. Up to 1995 the only available regeneration system in cassava was an organized type of somatic embryogenesis. Transformation of these organized somatic embryogenic cultures with particle bombardment or Agrobacterium tumefaciens resulted in chimeric transformed embryos. However, the transformed sector was lost after repeated cycles of secondary somatic embryogenesis. After 1995 a less organized system of somatic embryogenesis was developed, so called friable embryogenic callus (FEC) and a system of adventitious shoot regeneration. The FEC regeneration system was combined successfully with particle bombardment. Selection of transgenic plants was based on either luciferase activity, or resistance to the aminoglycoside paromomycin or the herbicide phosphinothricin. Furthermore, protoplasts of FEC are able to regenerate into plants and can be transformed by electroporation. The adventitious shoot regeneration system was combined successfully with Agrobacterium tumefaciens. For this mature somatic embryos were cocultivated with Agrobacterium and cultured for adventitious shoot development. After selection based on the aminoglycoside geneticin or on hygromycin transgenic plants were formed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002970925897
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  • 8
    Electronic Resource
    Electronic Resource
    Induction, germination and shoot development of somatic embryos in cassava (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 151-156 
    Details
    ISSN: 1573-5044
    Keywords: cassava ; plant regeneration ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four Indonesian and two Latin-American cassava genotypes (Manihot esculenta Crantz), were evaluated for their ability to develop somatic embryos from young leaf lobes. All genotypes formed somatic embryos but they differed in the frequency of embryos induced. The best genotypes, M. Col 22 and Tjurug, produced germinating embryos (GE) on 81% (22.1 GE/initial leaf lobe) and 46% (4.3 GE/initial leaf lobe) of the cultured leaf lobes, respectively. Up to 57% of the germinating embryos of M. Col 22 and 12% of Tjurug produced either normal or malformed shoots. Most malformed shoots developed into shoots with normal morphology after prolonged culture. All shoots formed roots after transfer to medium without BAP. Roots of all normal and most malformed regenerants had the original ploidy level (2n=36). Regardless of whether the plants were multipliedin vitro (150 plants) or in the greenhouse (30 plants) there were no morphological differences compared to parent plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01983228
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  • 9
    Electronic Resource
    Electronic Resource
    Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato (1988)
    Springer
    Potato research 31 (1988), S. 241-246 
    Details
    ISSN: 1871-4528
    Keywords: minitubers ; starch composition ; mutant detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Small amounts of potato tuber and leaf tissues are extracted in perchloric acid. After staining with I2-KI solution absorbancies at 618 and 550 nm are measured. The amylose/amylopectin ratio can be estimated from the ratio of the absorbancies by using a formula or a graph in which the specific absorptions of the two compounds are introduced. Weighing of samples is not required. The method can be used for the estimation of the starch composition of minitubers and is especially suitable for the detection of mutants with an altered starch composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02365532
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