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Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato

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Summary

Small amounts of potato tuber and leaf tissues are extracted in perchloric acid. After staining with I2-KI solution absorbancies at 618 and 550 nm are measured. The amylose/amylopectin ratio can be estimated from the ratio of the absorbancies by using a formula or a graph in which the specific absorptions of the two compounds are introduced. Weighing of samples is not required. The method can be used for the estimation of the starch composition of minitubers and is especially suitable for the detection of mutants with an altered starch composition.

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Authors and Affiliations

  1. Department of Genetics, University of Groningen, Center of Biological Sciences, Kerklaan 30, 9751 NN, Haren, the Netherlands

    J. H. M. Hovenkamp-Hermelink, J. N. De Vries, P. Adamse, E. Jacobsen & W. J. Feenstra

  2. Department of Biochemistry, University of Groningen, Nijenborgh 16, 9747 AG, Groningen, the Netherlands

    B. Witholt

Authors
  1. J. H. M. Hovenkamp-Hermelink
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  2. J. N. De Vries
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  3. P. Adamse
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  4. E. Jacobsen
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  5. B. Witholt
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  6. W. J. Feenstra
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Hovenkamp-Hermelink, J.H.M., De Vries, J.N., Adamse, P. et al. Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato. Potato Res 31, 241–246 (1988). https://doi.org/10.1007/BF02365532

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  • DOI: https://doi.org/10.1007/BF02365532

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