ISSN:
1573-5044
Keywords:
Colocasia esculenta
;
growth inhibition
;
in vitro storage
;
low temperature
;
mannitol
;
transfer regime
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The storage of a clone ofColocasia esculenta at 28/24°C over a 12-h photoperiod in the absence of mannitol, was not feasible with transfer intervals of more than eight months. Mannitol had a positive effect on survival at this temperature, but caused abnormalities at high concentrations. At 9°C in total darkness,C. esculenta could be stored for more than eight years with transfer intervals of approximately three years. After this period, more than 90% of the cultures showed living shoots, but not all shoots per culture showed regrowth. Cultures which were transferred three times during the experimental period, had a significantly (γ=0.05) lower number of regrowing shoots than cultures which were transferred twice. This might be due to the fact that the former cultures were transferred at a less favourable developmental stage. The addition of mannitol to the storage medium did not improve survival and regrowth, nor did a more gradual lowering of the temperature to 9°C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01983224
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