Summary
A high frequency plant regeneration system via organogenesis and somatic embryogenesis was established with callus cultures derived from mature zygotic embryos of different leek genotypes (Allium ampeloprasum L.). Four different callus types with varying morphogenetic potential were obtained. Relatively high concentrations of the auxin 2,4-dichlorophenoxy-acetic acid reduced callus weight and subsequent shoot regeneration and primordia formation of the callus. Shoot regeneration and primordia formation of the callus decreased after prolonged subculture on media containing 2,4-dichlorophenoxy acetic acid. A callus growth period of six weeks on Murashige and Skoog medium with 0.25–0.5 mg l-1 2,4-dichlorophenoxy acetic acid showed the highest rate of shoot regeneration after transfer of callus to regeneration medium with 1 mg l-1 kinetin.
Differences between leek genotypes in callus type, callus weight, shoot regeneration and primordia formation were observed. Histological observations showed that plant regeneration took place, both via the pathway of somatic embryogenesis and organogenesis.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- MS:
-
Murashige and Skoog (1962) medium
References
Abo El Nil, M.M., 1977. Organogenesis and embryogenesis in callus cultures of garlic (Allium sativum L.). Plant Sci Lett 9: 259–264.
Baumunk-Wende, E., 1989. Application of tissue culture in leek breeding (Allium porrum L.). Gartenbauwissenschaft 54: 20–24.
Bhaskaran, S. & R.Smith, 1990. Cell biology and molecular genetics; regeneration in cereal tissue culture. Crop Sci 30: 1328–1336.
Brown, D.C. & T.A.Thorpe, 1984. Plant regeneration by organogenesis. In: I.K.Vasil (Ed.), Cell Culture and Somatic Cell Genetics of Plants, Vol. 3, pp. 49–65. Academic Press, Orlando.
Buiteveld, J., P.van derValk, J.Jansen, J.Creemers-Molenaar & C.M.Colijn-Hooymans, 1993. Callus induction and plant regeneration from explants of commercial cultivars of leek (Allium ampeloprasum var. porrum L.). Plant Cell Rep 12: 431–434.
Debergh, P. & R.Standaert-De Metsenaere, 1976. Neoformation of bulbils in Allium porrum L. cultured in in vitro. Sci Hort 5: 11–12.
Doré, C., 1988. Multiplication végétative et conservation in vitro chez le poireau (Allium porrum L.). Agronomie 8: 509–511.
Dunstan, D.I. & K.C.Short, 1977. In vitro studies on organogenesis and growth in Allium cepa tissue cultures. Acta Hort 78: 139–148.
Dunstan, D.I. & K.C.Short, 1978. Shoot production from onion callus tissue cultures. Sci Hort 9: 99–110.
Dunstan, D.I. & K.C.Short, 1979. Shoot production from cultured Allium porrum tissues. Sci Hort 11: 37–43.
Emons, A.M.C. & H.deDoes, 1993. Origin and development of embryo and bud primordia during maturation of embryogenic calli of Zea mays L. Can J Bot 71: 1349–1356.
Emons, A.M.C., 1994. Somatic embryogenesis: cell biological aspects. Acta Bot Neerl 43 (1): 1–14.
Feder, N. & T.P.O'Brian, 1968. Plant microtechnique: some principles and new methods. Amer J Bot 55: 123–142.
Haccius, B., 1978. Question of unicellular origin of non zygotic embryos in callus cultures. Phytomorphology 28: 74–81.
Havel, L. & F.J.Nov]'ak, 1988. Regulation of somatic embryogenesis and organogenesis in Allium carinatum L. J Plant Physiol 132: 373–377.
Keller, J., 1992. In vitro cultivation of Allium species—a method for application in plant breeding and germplasm conservation. In: P. Hanelt, K. Hammer & H. Knupffer (Eds), The Genus Allium-Taxonomic Problems and Genetic Resources, pp. 137–152. Proc. International Symposium Gatersleben June 11–13, 1991.
Lu, C.C., L.Currah & E.B.Peffley, 1989. Somatic embryogenesis and plant regeneration in diploid Allium fistulosum x A. cepa F1 hybrid onions. Plant Cell Rep 7: 696–700.
Murashige, T. & F.Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497.
Nabors, M.W., J.W.Heyser, T.A.Dykes & K.J.DeMott, 1983. Long duration high frequency plant regeneration from cereal tissue cultures. Planta 157: 385–391.
Novák, F.J. & L.Havel, 1981. Shoot production from in vitro cultured flower heads of Allium porrum L. Biol Plant. 23 (4): 266–269.
Novák, F.J., L.Havel & J.Dolezel, 1986a. Onion, garlic and leek (Allium species). In: Y.P.S.Bajaj (Ed.), Biotechnology in Agriculture and Forestry, vol. 2, Crops I, pp. 387–408. Springer Verlag, Berlin, Heidelberg, New York, Tokyo.
Novák, F.J., L.Havel & J.Dolezel, 1986b. Allium. In: D.A.Evans, W.R.Sharp & P.V.Ammirato (Eds), Handbook of Plant Cell Culture, Vol. 4, pp. 419–456. MacMillan, New York.
Novák, F.J., 1990. Allium tissue culture. In: H.D.Rabinowitch & J.L.Brewster (Eds), Onions and Allied Crops, Vol. I, pp. 234–250. CRC Press, Inc., Boca Raton, Florida.
Phillips, G.C. & K.J.Luteyn, 1983. Effects of picloran and other auxins on onion tissue cultures. J Amer Soc Hort Sci 108: 948–953.
Pierik, R.L.M., 1987. In Vitro Culture of Higher Plant. Martinus Nijhoff Publishers, Dordrecht.
Rauber, M. & J.Grunewaldt, 1988. In vitro regeneration in Allium species. Plant Cell Rep 7: 426–429.
Schavemaker, T. & E.Jacobsen, 1995. Development of a cyclic somatic embryogenesis regeneration system for leek (Allium ampeloprasum L.) using zygotic embryos. Plant Cell Rep 14: 227–231.
Shahin, A.E. & K.Kaneko, 1986. Somatic embryogenesis and plant regeneration from callus cultures of non bulbing onions. Hort Sci 21 (2): 294–295.
Van Doorne, L.E., A.J. Stolte & A.M. van Harten, 1988. Application of in vitro techniques for multiplying and breeding leek (Allium porrum), 4th EUCARPIA Allium Symposium 1988.
Van derValk, P., O.E.Scholten, R.C.Verstappen & J.J.Dons, 1992. High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species. Plant Cell Tissue Org Cult 30: 181–191.
Vasil, I.K., 1985. Somatic embryogenesis and its consequences in the Gramineae. In: R.R.Henke, K.W.Hughes, M.J.Constantin & A.Hollaender (Eds), Tissue Culture in Forestry and Agriculture, pp. 31–47. Plenum Press, New York.
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Silvertand, B., van Rooyen, A., Lavrijsen, P. et al. Plant regeneration via organogenesis and somatic embryogenesis in callus cultures derived from mature zygotic embryos of leek (Allium ampeloprasum L.). Euphytica 91, 261–270 (1996). https://doi.org/10.1007/BF00033086
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DOI: https://doi.org/10.1007/BF00033086