Publication Date:
2013-07-19
Description:
The tyrosine phosphatase SHP2, encoded by PTPN11, is required for the survival, proliferation and differentiation of various cell types. Germline activating mutations in PTPN11 cause Noonan syndrome, whereas somatic PTPN11 mutations cause childhood myeloproliferative disease and contribute to some solid tumours. Recently, heterozygous inactivating mutations in PTPN11 were found in metachondromatosis, a rare inherited disorder featuring multiple exostoses, enchondromas, joint destruction and bony deformities. The detailed pathogenesis of this disorder has remained unclear. Here we use a conditional knockout (floxed) Ptpn11 allele (Ptpn11(fl)) and Cre recombinase transgenic mice to delete Ptpn11 specifically in monocytes, macrophages and osteoclasts (lysozyme M-Cre; LysMCre) or in cathepsin K (Ctsk)-expressing cells, previously thought to be osteoclasts. LysMCre;Ptpn11(fl/fl) mice had mild osteopetrosis. Notably, however, CtskCre;Ptpn11(fl/fl) mice developed features very similar to metachondromatosis. Lineage tracing revealed a novel population of CtskCre-expressing cells in the perichondrial groove of Ranvier that display markers and functional properties consistent with mesenchymal progenitors. Chondroid neoplasms arise from these cells and show decreased extracellular signal-regulated kinase (ERK) pathway activation, increased Indian hedgehog (Ihh) and parathyroid hormone-related protein (Pthrp, also known as Pthlh) expression and excessive proliferation. Shp2-deficient chondroprogenitors had decreased fibroblast growth factor-evoked ERK activation and enhanced Ihh and Pthrp expression, whereas fibroblast growth factor receptor (FGFR) or mitogen-activated protein kinase kinase (MEK) inhibitor treatment of chondroid cells increased Ihh and Pthrp expression. Importantly, smoothened inhibitor treatment ameliorated metachondromatosis features in CtskCre;Ptpn11(fl/fl) mice. Thus, in contrast to its pro-oncogenic role in haematopoietic and epithelial cells, Ptpn11 is a tumour suppressor in cartilage, acting through a FGFR/MEK/ERK-dependent pathway in a novel progenitor cell population to prevent excessive Ihh production.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148013/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4148013/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yang, Wentian -- Wang, Jianguo -- Moore, Douglas C -- Liang, Haipei -- Dooner, Mark -- Wu, Qian -- Terek, Richard -- Chen, Qian -- Ehrlich, Michael G -- Quesenberry, Peter J -- Neel, Benjamin G -- 8P20GM103468/GM/NIGMS NIH HHS/ -- NIH R21AR57156/AR/NIAMS NIH HHS/ -- P20 RR025179/RR/NCRR NIH HHS/ -- R21 AR057156/AR/NIAMS NIH HHS/ -- R37CA49152/CA/NCI NIH HHS/ -- England -- Nature. 2013 Jul 25;499(7459):491-5. doi: 10.1038/nature12396. Epub 2013 Jul 17.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Orthopaedics, Brown University Alpert Medical School and Rhode Island Hospital, Providence, Rhode Island 02903, USA. wyang@lifespan.org〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23863940" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bone Neoplasms/drug therapy/genetics/*metabolism/*pathology
;
Cartilage/metabolism/pathology
;
Cathepsin K/deficiency/genetics/metabolism
;
Cell Division
;
Cell Lineage
;
Chondromatosis/drug therapy/genetics/*metabolism/*pathology
;
Exostoses, Multiple Hereditary/drug therapy/genetics/*metabolism/*pathology
;
Fibroblast Growth Factors/metabolism
;
Gene Deletion
;
Gene Expression Regulation/drug effects
;
Genes, Tumor Suppressor/physiology
;
Hedgehog Proteins/antagonists & inhibitors/*metabolism
;
MAP Kinase Signaling System
;
Macrophages/metabolism
;
Mesenchymal Stromal Cells/cytology/*metabolism
;
Mice
;
Mice, Knockout
;
Mice, Transgenic
;
Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors/metabolism
;
Monocytes/metabolism
;
Osteoclasts/metabolism
;
Osteopetrosis/genetics/metabolism/pathology
;
Parathyroid Hormone-Related Protein/metabolism
;
Protein Tyrosine Phosphatase, Non-Receptor Type
;
11/*deficiency/genetics/metabolism
;
*Signal Transduction/drug effects
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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