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  • 1
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    Advances in Plant Taxonomy and Systematics (2023)
    MDPI - Multidisciplinary Digital Publishing Institute
    Details
    Publikationsdatum: 2023-08-08
    Beschreibung: This reprint of the Special Issue "Advances in Plant Taxonomy and Systematics", includes an Editorial and fifteen high-quality papers published between April 2022 and April 2023 by global researchers. This collection addresses scholars and students interested in current progresses in taxonomy and systematics, which are crucial for all plant applications.
    Schlagwort(e): alpine species ; chromosome number ; Irano-Turanian region ; biogeography ; rapid radiation ; Veronica ; endemism ; morphometrics ; image analysis ; molecular analysis ; niche similarity ; nomenclature ; Magnoliaceae ; chloroplast genome ; phylogenomics ; intergeneric relationship ; Crassula ; Crassulaceae ; plastome ; codon usage ; codon aversion ; DNA barcoding ; evolutionary rates ; phylogeny ; Adonanthe ; molecular phylogeny ; taxonomy ; steppic plant ; demographic systematics ; ethology ; evolution ; natural selection ; next-generation sequencing ; ordination ; reproductive isolation ; sexual deceit ; speciation ; species circumscription ; Astragalus subgenus Hypoglottis ; Leguminosae ; Iran ; rapid diversification ; section Hypoglottidei ; section Stereothrix ; Australia ; carnivorous plants ; non-core Caryophyllales ; Nepenthales ; sundews ; typification ; Adelieae ; Argythamnia ; Caperonia ; Caperonieae ; Chiropetalum ; Ditaxeae ; Ditaxis ; phylogenetics ; Philyra ; Arecaceae ; Bentinckia ; biogeographic analysis ; karyomorphology ; Asteraceae ; ecology ; ddRADseq ; geography ; leaf morphology ; polyploidy ; chromosome numbers ; Crocus heuffelianus group ; Crocus series Verni ; dysploidy ; genome size ; genotyping-by-sequencing (GBS) ; morphometry ; apomixis ; hybridisation ; multiple origins ; Sorbus austriaca ; genomics ; geometric morphometrics ; Ranunculus auricomus ; taxonomically complex groups (TCGs) ; arid lands ; Cactaceae ; colonization ; Mammillaria ; Mexican Plateau ; Miocene ; Pleistocene ; recent diversification ; n/a ; bic Book Industry Communication::G Reference, information & interdisciplinary subjects::GP Research & information: general ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PST Botany & plant sciences
    Sprache: Englisch
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    E-BOOK
  • 2
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    Filamentous Bacteriophage in Bio/Nano/Technology, Bacterial Pathogenesis and Ecology (2021)
    Frontiers Media SA
    Details
    Publikationsdatum: 2024-04-05
    Beschreibung: Filamentous phage (genus Inovirus) infect almost invariably Gram-negative bacteria. They are distinguished from all other bacteriophage not only by morphology, but also by the mode of their assembly, a secretion-like process that does not kill the host. “Classic” Escherichia coli filamentous phage Ff (f1, fd and M13) are used in display technology and bio/nano/technology, whereas filamentous phage in general have been put to use by their bacterial hosts for adaptation to environment, pathogenesis, biofilm formation, horizontal gene transfer and modulating genome stability. Many filamentous phage have a “symbiotic” life style that is often manifested by inability to form plaques, preventing their identification by standard phage-hunting techniques; while the absence or very low sequence conservation between phage infecting different species often complicates their identification through bioinformatics. Nevertheless, the number of discovered filamentous phage is increasing rapidly, along with realization of their significance. “Temperate” filamentous phage whose genomes are integrated into the bacterial chromosome of pathogenic bacteria often modulate virulence of the host. The Vibrio cholerae phage CTXf genome encodes cholera toxin, whereas many filamentous prophage influence virulence without encoding virulence factors. The nature of their effect on the bacterial pathogenicity and overall physiology is the next frontier in understanding intricate relationship between the filamentous phage and their hosts. Phage display has been widely used as a combinatorial technology of choice for discovery of therapeutic antibodies and peptide leads that have been applied in the vaccine design, diagnostics and drug development or targeting over the past thirty years. Virion proteins of filamentous phage are integral membrane proteins prior to assembly; hence they are ideal for display of bacterial surface and secreted proteins. The use of this technology at the scale of microbial community has potential to identify host-interacting proteins of uncultivable or low-represented community members. Recent applications of Ff filamentous phage extend into protein evolution, synthetic biology and nanotechnology. In many applications, phage serves as a monodisperse long-aspect nano-scaffold of well-defined shape. Chemical or chenetic modifications of this scaffold are used to introduce the necessary functionalities, such as fluorescent labels, ligands that target specific proteins, or peptides that promote formation of inorganic or organic nanostructures. We anticipate that the future holds development of new strategies for particle assembly, site-specific multi-functional modifications and improvement of existing modification strategies. These improvements will render the production of filamentous-phage-templated materials safe and affordable, allowing their applications outside of the laboratory.
    Schlagwort(e): QR1-502 ; Q1-390 ; pathogenic bacteria ; filamentous bacteriophage ; phage display ; Glioblastoma ; Liposome ; Vaccine ; microbial communities ; dip-stick ; chemical modification ; Nanorods ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSG Microbiology (non-medical)
    Sprache: Englisch
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  • 3
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    New combinations in Senegalia and Vachellia (Leguminosae: Mimosoideae) for Southeast Asia and China (2013)
    In:  Blumea - Biodiversity, Evolution and Biogeography of Plants (0006-5196) vol.58 (2013) nr.1 p.39
    Details
    Publikationsdatum: 2015-03-06
    Beschreibung: Fragmentation of the former broadly circumscribed genus Acacia (Leguminosae: Mimosoideae) necessitates combinations in Senegalia (syn. Acacia subg. Aculeiferum) and Vachellia (syn. former Acacia subg. Acacia) for the following 40 taxa that are recorded for the region encompassed by Southeast Asia and China. Senegalia andamanica, S. borneensis, S. caesia, S. comosa, S. delavayi, S. delavayi var. kunmingensis, S. donnaiensis, S. gageana, S. intsia, S. kekapur, S. kostermansii, S. meeboldii, S. megaladena, S. megaladena var. garrettii, S. megaladena var. indochinensis, S. merrillii, S. palawanensis, S. pennata subsp. hainanensis, S. pennata subsp. insuavis, S. pluricapitata, S. pluriglandulosa, S. pruinescens, S. pseudointsia, S. sulitii, S. tawitawiensis, S. teniana, S. thailandica, S. tonkinensis, S. torta, S. verheijenii, S. vietnamensis and S. yunnanensis. Vachellia harmandiana, V. inopinata, V. kingii, V. leucophloea, V. leucophloea var. microcephala, V. myaingii, V. siamensis (syn. Acacia craibii) and V. tomentosa. Senegalia rugata replaces the well-known name Acacia concinna. There are four doubtful names for the region, namely, Acacia brunnescens, A. microcephala, A. obcordata and A. pseudoarabica.
    Schlagwort(e): Acacia ; China ; Leguminosae ; Mimosoideae ; SE Asia ; Senegalia ; Vachellia
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: Article / Letter to the editor
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  • 4
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    New combinations in Senegalia and Vachellia (Leguminosae: Mimosoideae) for Southeast Asia and China (2013)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 58 no. 1, pp. 39-44
    Details
    Publikationsdatum: 2024-04-10
    Beschreibung: Fragmentation of the former broadly circumscribed genus Acacia (Leguminosae: Mimosoideae) necessitates combinations in Senegalia (syn. Acacia subg. Aculeiferum) and Vachellia (syn. former Acacia subg. Acacia) for the following 40 taxa that are recorded for the region encompassed by Southeast Asia and China. Senegalia andamanica, S. borneensis, S. caesia, S. comosa, S. delavayi, S. delavayi var. kunmingensis, S. donnaiensis, S. gageana, S. intsia, S. kekapur, S. kostermansii, S. meeboldii, S. megaladena, S. megaladena var. garrettii, S. megaladena var. indochinensis, S. merrillii, S. palawanensis, S. pennata subsp. hainanensis, S. pennata subsp. insuavis, S. pluricapitata, S. pluriglandulosa, S. pruinescens, S. pseudointsia, S. sulitii, S. tawitawiensis, S. teniana, S. thailandica, S. tonkinensis, S. torta, S. verheijenii, S. vietnamensis and S. yunnanensis. Vachellia harmandiana, V. inopinata, V. kingii, V. leucophloea, V. leucophloea var. microcephala, V. myaingii, V. siamensis (syn. Acacia craibii) and V. tomentosa. Senegalia rugata replaces the well-known name Acacia concinna. There are four doubtful names for the region, namely, Acacia brunnescens, A. microcephala, A. obcordata and A. pseudoarabica.
    Schlagwort(e): Acacia ; China ; Leguminosae ; Mimosoideae ; SE Asia ; Senegalia ; Vachellia
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
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  • 5
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    Cynometra cebuensis, a new species of Leguminosae (Caesalpinioidae) from the Philippines (2013)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 58 no. 1, pp. 18-20
    Details
    Publikationsdatum: 2024-04-10
    Beschreibung: A new species of Cynometra from the Tabunan forest on Cebu island in the Philippines is described.
    Schlagwort(e): Caesalpinioidae ; Cebu ; Cynometra cebuensis ; endemism ; Leguminosae ; Tabunan forest
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
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  • 6
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    Notes on Malesian Fabaceae (Leguminosae-Papilionoideae) 15. Notes on Indigofera (2011)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 56 no. 3, pp. 270-272
    Details
    Publikationsdatum: 2024-01-12
    Beschreibung: Notes on some morphological features of Indigofera as well as taxonomic notes on several species are given. The recently described I. erectifructa is reduced to a synonym of I. luzoniensis.
    Schlagwort(e): Fabaceae ; Indigofera ; Leguminosae ; Malesia ; Papilionoideae
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
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  • 7
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    A natural infrageneric classification for Cicer (Leguminosae, Cicereae) (2007)
    In:  Blumea - Biodiversity, Evolution and Biogeography of Plants (0006-5196) vol.52 (2007) nr.2 p.379
    Details
    Publikationsdatum: 2015-03-06
    Beschreibung: A comprehensive morphological survey and analysis of all taxonomically recognised wild species of Cicer L. (Leguminosae, Cicereae) is presented. The data (104 characters from 152 herbarium specimens representing 34 of the 44 recognised taxa in the genus Cicer with supplementary data for the remaining taxa taken from the literature) were analysed using multivariate statistics (cluster analysis, factor analysis and ordination techniques). The results are discussed in the context of extant classifications and the re-organisation of a novel infrageneric classification also incorporating information from published genetic data. A revised classification with 3 subgenera, 5 sections and 2 series is proposed.
    Schlagwort(e): Leguminosae ; Cicereae ; infrageneric classification ; morphology ; wild chickpeas
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: Article / Letter to the editor
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  • 8
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    A new species of Dalbergia (Leguminosae, Dalbergieae) from Western Africa (2007)
    In:  Blumea - Biodiversity, Evolution and Biogeography of Plants (0006-5196) vol.52 (2007) nr.1 p.11
    Details
    Publikationsdatum: 2015-03-06
    Beschreibung: Dalbergia hepperi from Western Africa is described and illustrated. The combination of glabrous ovaries, flat and glabrous fruits and ovate to obovate leaflets with a conspicuous acuminate apex is not known from any other Dalbergia species from this region.
    Schlagwort(e): Leguminosae ; Dalbergia ; Africa ; Liberia ; Sierra Leone ; taxonomy
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: Article / Letter to the editor
    Format: application/pdf
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  • 9
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    Notes on Malesian Fabaceae (Leguminosae–Papilionoideae). 13. The genus Inocarpus (2007)
    In:  Blumea - Biodiversity, Evolution and Biogeography of Plants (0006-5196) vol.52 (2007) nr.2 p.401
    Details
    Publikationsdatum: 2015-03-06
    Beschreibung: The genus Inocarpus J.R. & G. Forst. is revised. Three species are recognized of which one is new (I. glabellus Adema). A key to the species is provided.
    Schlagwort(e): Inocarpus ; Leguminosae ; Papilionoideae ; Dalbergieae ; Malesia ; Pacific
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: Article / Letter to the editor
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  • 10
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    A natural infrageneric classification\nfor Cicer (Leguminosae, Cicereae) (2007)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 52 no. 2, pp. 379-400
    Details
    Publikationsdatum: 2024-01-12
    Beschreibung: A comprehensive morphological survey and analysis of all taxonomically recognised wild species of Cicer L. (Leguminosae, Cicereae) is presented. The data (104 characters from 152 herbarium specimens representing 34 of the 44 recognised taxa in the genus Cicer with supplementary data for the remaining taxa taken from the literature) were analysed using multivariate statistics (cluster analysis, factor analysis and ordination techniques). The results are discussed in the context of extant classifications and the re-organisation of a novel infrageneric classification also incorporating information from published genetic data. A revised classification with 3 subgenera, 5 sections and 2 series is proposed.
    Schlagwort(e): Leguminosae ; Cicereae ; infrageneric classification ; morphology ; wild chickpeas
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
    Format: application/pdf
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  • 11
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    A new species of Dalbergia (Leguminosae, Dalbergieae) from Western Africa (2007)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 52 no. 1, pp. 11-14
    Details
    Publikationsdatum: 2024-01-12
    Beschreibung: Dalbergia hepperi from Western Africa is described and illustrated. The combination of glabrous ovaries, flat and glabrous fruits and ovate to obovate leaflets with a conspicuous acuminate apex is not known from any other Dalbergia species from this region.
    Schlagwort(e): Leguminosae ; Dalbergia ; Africa ; Liberia ; Sierra Leone ; taxonomy
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
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  • 12
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    Notes on Malesian Fabaceae (Leguminosae\xe2\x80\x93Papilionoideae). 13. The\ngenus Inocarpus (2007)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 52 no. 2, pp. 401-407
    Details
    Publikationsdatum: 2024-01-12
    Beschreibung: The genus Inocarpus J.R. & G. Forst. is revised. Three species are recognized of which one is new (I. glabellus Adema). A key to the species is provided.
    Schlagwort(e): Inocarpus ; Leguminosae ; Papilionoideae ; Dalbergieae ; Malesia ; Pacific
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
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  • 13
    Digitale Medien
    Digitale Medien
    In Vivo Gene Transfer to the Mouse Nasal Cavity Mucosa Using a Stable Cationic Lipid Emulsion (2000)
    Springer
    Molecules and cells 10 (2000), S. 142-147 
    Details
    ISSN: 0219-1032
    Schlagwort(e): DNA Delivery ; Heparin ; In Vivo Transfection ; Liposome ; Nasal Mucosa ; Physicochemical Property
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We evaluate a new cationic emulsion as a mucosal gene carrier and elucidate the relationship between the transfection efficiency and the stability of the carrier/DNA complex. A cationic lipid emulsion was formulated with soybean oil and 1,2-dioleoyl-sn-glycero-3-trimethylammonium-propane (DOTAP) as major components and was used to transfer genes to the epithelial cells of the mouse nasal cavity via intranasal instillation. Correlation between the transfection efficiency and the stability of the carrier/DNA complex was investigated by measuring the carrier size changes and by observing the degree of DNA protection against DNase I digestion in the presence of heparin. The cationic emulsion showed at least 3 times better transfection activity than the liposomal carriers in nasal mucosae. The cationic emulsion was stable in the presence of heparin whereas the liposomal carriers became very unstable. Unlike DNA in liposome/DNA complexes, DNA in the emulsion/DNA complex was resistant to heparin exchange and DNase I digestion. The cationic emulsion was more effective in delivering DNA to nasal mucosae than commercially available liposomal carriers. The transfection activities of the lipid carriers in nasal cavity mucosae are in agreement with the stability of the lipid carriers and their complexes with DNA.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s10059-000-0142-1
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  • 14
    Digitale Medien
    Digitale Medien
    Transfection of Lymantria dispar insect cell lines (2000)
    Springer
    Methods in cell science 22 (2000), S. 257-263 
    Details
    ISSN: 1573-0603
    Schlagwort(e): In vitro ; Liposome ; Lymantria dispar ; Transfection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Lepidopteran cell lines derived from the gypsy moth, Lymantria dispar, have not been widely used in protein expression studies or systems because they are weakly adherent, have specific growth requirements and characteristics, and are generally difficult to transfect. Using lipid-mediated transfection of a reporter plasmid, we modify the standard method for transfection of L. dispar-derived embryonic cell lines IPLB-LdEp and -LdEIta, obtaining transfection efficiencies of 34% and 30%, respectively, as determined by image analysis assays. Using the standard lipid-mediated method, we obtain transfection efficiencies for L. dispar-derived cell line IPLB-Ld652Y of at least 40% with high mean expression levels, indicating the IPLB-Ld652Y cell line may be a superior choice for expression studies or systems requiring L. dispar-derived cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1017533119177
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  • 15
    Digitale Medien
    Digitale Medien
    Single-flowered vetch (Vicia articulata Hornem.): A relic crop in Italy (2000)
    Springer
    Genetic resources and crop evolution 47 (2000), S. 461-465 
    Details
    ISSN: 1573-5109
    Schlagwort(e): C-banding ; crop genetic resources ; Leguminosae ; Sardinia ; seed quality ; Vicia articulata Hornem
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Single-flowered vetch (Vicia articulata Hornem.), a crop native of the Mediterranean area, is widespread mainly in south Europe, in west Asia and Australia for forage, green manure and human food. In Italy this crop was still cultivated in the 1950s but, later, no records concerning its cultivation were available. This strongly suggested to place V. articulata among the Italian crop species that disappeared in the 20th century. In the course of a collecting expedition in Sardinia, a relic population, mistaken for lentil, was found. This population has been characterised from a botanical, nutritional and cytological point of view. V. articulata appears to be lower in nutritional quality than lentil. However, within the framework of a sustainable agriculture, V. articulata could find interesting opportunities. In fact, its role in soil conservation and rescue of marginal areas could be interesting, as well as its potentialities as a source of useful genes in breeding programmes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008711022396
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  • 16
    Digitale Medien
    Digitale Medien
    In vitro organogenesis and plant formation in Acacia sinuata (2000)
    Springer
    Plant cell, tissue and organ culture 61 (2000), S. 23-28 
    Details
    ISSN: 1573-5044
    Schlagwort(e): Acacia sinuata ; Leguminosae ; regeneration ; tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In vitro morphogenesis via organogenesis was achieved from callus cultures derived from hypocotyl explants of Acacia sinuata on MS (Murashige and Skoog, 1962) medium. Calli were induced from hypocotyl explants excised from 7-day-old seedlings on MS medium containing 3% sucrose, 0.8% agar, 6.78 μM 2,4-dichlorophenoxyacetic acid and 2.22 μM 6-benzylaminopurine. Regeneration of adventitious buds from callus was achieved when they were cultured on MS medium supplemented with 10% coconut water, 13.2 μM 6-benzylaminopurine and 3.42 μM indoleacetic acid. Addition of gibberellic acid (1.73 μM) favored shoot elongation. Regenerated shoots produced prominent roots when transferred to half strength MS medium supplemented with 7.36 μM indolebutyric acid. Rooted plantlets, thus developed were hardened and successfully established in the soil. This protocol yielded an average of 20 plants per hypocotyl explant over a period of 4 months.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006442818277
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  • 17
    Digitale Medien
    Digitale Medien
    Purification and Properties of a Nucleotide Pyrophosphatase from Lentil Seedlings (2000)
    Springer
    The protein journal 19 (2000), S. 209-214 
    Details
    ISSN: 1573-4943
    Schlagwort(e): Lens esculenta ; Leguminosae ; nucleotide pyrophosphatase ; nucleotides
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract A nucleotide pyrophosphatase (EC 3.6.1.9) was purified to homogeneity from lentil seedlings. The enzyme is a single polypeptide chain of 75 ± 2 kDa that exhibits hydrolytic activities toward pyrophosphate linkages of several substrates. Reduced and oxidized forms of NAD(P) were shown to be hydrolyzed to nicotinamide mononucleotide and AMP. Other dinucleotides such as FAD and dinucleoside oligophosphates were hydrolyzed as well, but with lower efficiency. Pyrophosphatase activity was increased in the presence of divalent cations such as Ca2+, Mg2+, and Mn2+, whereas Cu2+, Zn2+, and Ni2+ ions inhibited this activity. The active site in the enzyme was not defined, but histidine residue(s) seemed to be crucial for the enzymatic activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1007007803996
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  • 18
    Digitale Medien
    Digitale Medien
    Secretory cavities and pellucid dots in leaflets ofLonchocarpus (Leguminosae, Papilionoideae, Millettieae) (2000)
    Springer
    Plant systematics and evolution 221 (2000), S. 61-68 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Papilionoideae ; Lonchocarpus ; leaflets ; secretory cavities ; false secretory cavities ; pellucid dots ; glands ; anatomy ; taxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The occurrence of leaflet secretory cavities in 23 Brazilian species ofLonchocarpus was surveyed anatomically and compared with data from external morphology (presence of pellucid dots) to evaluate their taxonomic significance. This study revealed three cases: presence of secretory cavities associated with pellucid dots, pellucid dots corresponding to false secretory cavities, and absence of pellucid dots and secretory cavities. These results indicate that in this genus the “glandular pellucid dots” cited in morphological descriptions do not always correspond to secretory cavities, and that their nature must always be confirmed by histological sections. Secretory cavities have systematic significance at subgeneric taxonomic level for the genusLonchocarpus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01086380
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  • 19
    Digitale Medien
    Digitale Medien
    Catalase Activity of Nodules in the Ureide- and Amide-transporting Legume Plants (2000)
    Springer
    Russian journal of plant physiology 47 (2000), S. 721-727 
    Details
    ISSN: 1608-3407
    Schlagwort(e): Leguminosae ; symbiotic nitrogen fixation ; effective and ineffective symbiosis ; root nodules ; catalase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The relations of catalase activity to the efficiency of symbiotic dinitrogen fixation and leghemoglobin (Lb) content were investigated in roots and nodules of several legume plant species together with the catalase distribution between the inner bacteroidal and the outer cortical nodule tissues. The catalase activity in the nodules exceeded that of the roots of the amide- and ureide-synthesizing plant species by one and two orders of magnitude. During the growth period, catalase activity and Lb content changed in parallel and reached their highest levels early in the stage of flowering or fruit formation, depending on plant species. In the case of effective symbiosis, catalase activity in the nodules was 2.5–5 times higher than in the case of ineffective symbiosis. Catalase activity in the bacteroidal zone of the nodules was several times higher than that of the cortical tissue, and two nodule tissues differed in catalase activity more notably in the plant species exporting ureides. The authors suggest that high catalase activity in the nodules, especially in their bacteroidal zone, is essential for the efficient functioning of the symbiotic system of dinitrogen fixation in both ureide- and amide-transporting plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1026694709210
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  • 20
    Digitale Medien
    Digitale Medien
    Software dependent phenetic relationships of non-nodulating African species ofAcacia (2000)
    Springer
    Plant systematics and evolution 220 (2000), S. 139-146 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Acacia ; classification ; Leguminosae ; morphology ; phenetics ; RAPD ; software ; taxonomy ; UPGMA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The phenetic analysis of non-nodulatingAcacia species by Harrier et al. (1997) was repeated to illustrate how different computer programs may generate alternative UPGMA trees for the very same data, even in the absence of data input order effects (ties). For example, all Harrier et al.'s UPGMA dendrograms produced by software from the Scottish Agricultural Statistics Service differed from those obtained by the packages NTSYS and MVSP87. Particularly, the positions ofA. albida, A. rovumae, andA. pentagona, as well as the relationships betweenDiacanthae andTriacanthae were affected by this phenomenon. Hence, whenever clustering techniques are used, care should be taken to consider possible software-dependent caveats and artefacts. Nevertheless, all programs provided clusterings that largely coincided with the subgeneric and sectional groupings proposed by Vassal (1972) although the positions of some species varied depending on whether morphological or molecular data were considered (e.g.A. albida andA. rovumae).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985042
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  • 21
    Digitale Medien
    Digitale Medien
    Structure and property changes in certain materials influenced by the external qi of qigong (1999)
    Springer
    Materials research innovations 2 (1999), S. 349-359 
    Details
    ISSN: 1433-075X
    Schlagwort(e): Key words Qigong ; Qi ; Material structure ; Material property ; Raman ; Water ; Protein ; Liposome ; Saline ; Glucose
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau
    Notizen: Abstract  Temperature, time, pressure (or stress) are considered important factors in changing the Gibbs free energy and optimizing the structure and properties of materials during materials processing. The effects of some other variables, including the magnetic field, electrical field, electromagnetic and ultrasonic radiation, and chemical reactions have also been well characterized. These factors have been widely applied in materials processing, and their limitations have been discovered. Thus additional factors and innovative techniques are constantly being sought to overcome those limitations. This paper presents such an innovative technique called qigong. Three sets of materials-related experiments conducted by qigong doctor Yan and his collaborators are described in which for the first time the effects of qi on inanimate matter samples with no mechanical or electrical connection to the system are revealed on laboratory instruments. These experiments show that external qi of qigong produces significant structural changes in water and aqueous solutions, alters the phase behavior of dipalmitoyl phosphatidyl choline (DPPC) liposomes, and enables the growth of Fab protein crystals. These results demonstrate objective phenomena resulting from qigong and the potential of this ancient technology system, even in material processing. Important attributes of qi are summarized and the possible implications of these results from the materials perspective are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s100190050113
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  • 22
    Digitale Medien
    Digitale Medien
    Light scattering characterization of extruded lipid vesicles (1999)
    Springer
    European biophysics journal 28 (1999), S. 187-199 
    Details
    ISSN: 1432-1017
    Schlagwort(e): Key words Membrane ; Liposome ; Morphology ; Elongation ; Osmotic effect
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Physik
    Notizen: Abstract By modeling extruded unilamellar lipid vesicles as thin-walled ellipsoidal shells, mathematical analysis provides simple equations which relate the mean elongation and other morphological characteristics of a vesicle population to quantities readily obtained from combined static and dynamic light scattering measurements. For SOPC vesicles extruded through a 100 nm pore-size filter into a 72.9 mm NaCl solution, the inferred elongation ratio (vesicle long axis to short axis) is approximately 3.7±0.6. When these vesicles were dialyzed into hypertonic or hypotonic solutions, this elongation ratio varied from 1 (for spherical liposomes) in strongly hypotonic solutions to greater than 6 in increasingly hypertonic solutions, beyond which abrupt morphological transformations appear. These results are quantitatively consistent with a mechanism of vesicle formation by extrusion and with the expectation that vesicle volumes change to equalize internal and external osmolarity via water flow, subject to the constraint of constant bilayer area. Our analysis also provides simplified equations to assess the effects of vesicle elongation and polydispersity on liposome parameters that are commonly required to characterize vesicle preparations for diverse applications. The implications of this study for routine light scattering characterization of extruded vesicles are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002490050199
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  • 23
    Digitale Medien
    Digitale Medien
    An improved method for the purification of stellate cells from rat liver with Dichloromethylene Diphosphate (CL2MDP) (1999)
    Springer
    Methods in cell science 21 (1999), S. 19-24 
    Details
    ISSN: 1573-0603
    Schlagwort(e): Dichloromethylene diphosphate ; Hepatic stellate cell isolation ; Liposome ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Hepatic perisinusoidal cell population consists of hepatic stellate cells, Kupffer cells, endothelial cells, and Pit cells. These cells are isolated by enzymic digestion and purified by density gradient centrifugation. With isolation of stellate cells, conventional method is unable to eliminate the contamination of Kupffer cells because the densities of these two cells are similar. We report here an improved method for isolation of highly purified hepatic stellate cells, using dichloromethylene diphosphate (CL2MDP), which has selective cytotoxicity of Kupffer cells. Three days after the single intravenous administration of liposome-encapsulated CL2MDP, the Kupffer cells disappeared almost completely from the liver. Following Percoll density gradient centrifugation, the purity of the hepatic stellate cells exceeded 98% without any contamination of the Kupffer cells. Kupffer cells are reported to affect the physiological functions of stellate cells. The availability of highly purified stellate cells will facilitate the investigation of their functions in primary culture.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009872219428
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  • 24
    Digitale Medien
    Digitale Medien
    Production of flavonoids by Psoralea hairy root cultures (1999)
    Springer
    Plant cell, tissue and organ culture 56 (1999), S. 96-103 
    Details
    ISSN: 1573-5044
    Schlagwort(e): cell line establishment ; coumestrol ; daidzein ; flavonoid ; hairy root ; Leguminosae ; Psoralea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Eighteen transformed root cultures from 7 Psoralea plant species (Leguminosae) were established with the objective of producing daidzein and related flavonoids. All the 18 hairy root lines grew fast and had the same capacities for biomass production. Each of them produced daidzein as an intracellular secondary metabolite. The Lach5 hairy root line, obtained from P. lachnostachys, was a high producing line for daidzein and was further studied for biomass and flavonoid production. This root line showed exponential growth. Chitosan was used for elicitation purposes as well as for its permeabilizing effect. Little elicitation effect could be demonstrated and the metabolite release in the medium was weak (about 1%) and limited to the first 29 h after chitosan addition. Daidzein was demonstrated to be more concentrated in young parts (apexes) whereas coumestrol content was higher in older parts (brown tissues). Compared to callus cultures from the same plant species, hairy roots displayed comparable concentrations. However, high-producing lines were more frequently found with hairy roots (4 out of 18) than with callus cultures (4 out of 217)
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006206104795
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  • 25
    Digitale Medien
    Digitale Medien
    Reproductive biology ofCaesalpinia calycina andC. pluviosa (Leguminosae) of the caatinga of north-eastern Brazil (1999)
    Springer
    Plant systematics and evolution 217 (1999), S. 43-53 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Caesalpinia ; Angiosperms ; bee-pollination ; andromonoecy ; late-acting self-incompatibility ; fruiting success
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The pollination biology, breeding system and fruiting success ofCaesalpinia calycina andC. pluviosa var.sanfranciscana were studied in caatinga vegetation in Bahia, NE Brazil. The principal pollinators for both species were carpenter bees.Caesalpinia calycina is andromonoecious but inC. pluviosa all flowers are hermaphrodite. InC. calycina all selfed flowers were abscised within 72 h despite rapid self-pollen tube growth to the ovary and ovule penetration. Prevention of selfing therefore seems to be controlled by a post-zygotic mechanism. Both species had very low fruit-set and it is suggested that this is at least in part due to geitonogamous pollinations with ovule penetration by self pollen tubes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984921
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  • 26
    Digitale Medien
    Digitale Medien
    Pollination mechanisms and pollen-ovule ratios in someGenisteae (Fabaceae) from Southwestern Europe (1999)
    Springer
    Plant systematics and evolution 216 (1999), S. 23-47 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Genisteae ; Spain ; nectar ; pollen ; pollination mechanisms ; pollen/ovule ratios ; anther biomass
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We studied the biology and floral rewards of 34 taxa ofGenisteae from the SW of Europe. Most of the floral attributes show a statistically significant direct relationship. Anther biomass of the lower whorl (lW) is significantly lower than that of the upper whorl (uW), and the ratio of the two (Rv) differs among the taxa. All taxa are polliniferous, andRetama sphaerocarpa also secretes nectar. They can be considered xenogamous or facultative xenogams on the basis of their high pollen/ovule (P/O) ratios. Three principal mechanisms of pollen releasing are identified in this tribe: valvular, pump and explosive; the latter comprises specialized and nonspecialized subtypes. Pollination is sternotribic except in the specialized explosive mechanism, in which it is noto-sternotribic. Thus some pollen serves as food (pollen from the uW, which adheres to the ventral surface of the insect) and part of the pollen fulfils a polliniferous function (pollen from the lW, which adheres to the dorsal surface). Species that use a pump mechanism have very low Rv values (Rv=0.08−0.26); species with valvular or nonspecialized explosive mechanisms have Rv values between 0.24 and 0.58; those with a specialized mechanism of pollen presentation have high Rv values (0.6−0.76). In contrast to expectations, the highest P/O ratios appear in the specialized explosive system, which allows a single visit from the insect.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985099
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  • 27
    Digitale Medien
    Digitale Medien
    Floral ontogeny in tribe Dalbergieae (Leguminosae: Papilionoideae):Dalbergia brasiliensis, Machaerium villosum s. l.Platymiscium floribundum, andPterocarpus rotundifolius (1999)
    Springer
    Plant systematics and evolution 219 (1999), S. 1-25 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Fabaceae ; Leguminosae ; Dalbergieae ; Dalbergia brasiliensis ; Machaerium villosum ; Platymiscium floribundum ; Pterocarpus rotundifolius ; Ontogeny ; flower ; initiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Floral organogenesis and development of the tropical legume treesDalbergia brasiliensis, Machaerium villosum, Platymiscium floribundum, andPterocarpus rotundifolius were studied using scanning electron microscopy. The aims were to compare ontogenies and to elucidate if floral ontogenetic data will provide new character states diagnostic of the tribe Dalbergieae, which is considered a basal papilionoid tribe and primarily defined on fruit characters. Organ inception is principally acropetal in all taxa studied. Carpel inception is, however, consistently precocious. InD. brasiliensis sepals are initiated in an order not previously reported in Papilionoideae. It may be considered modified helical. InP. rotundifolius the inner whorl of stamens initiate in an unusual way, this is lateral two stamens first, then the two abaxial ones, and last the adaxial one, opposed to the unidirectional order usually seen in Papilionoideae. Generally the differences in flower development among the studied genera appear at initiation and late stage in ontogenesis, rather than at mid-stage.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01090296
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  • 28
    Digitale Medien
    Digitale Medien
    Investigation and documentation of hybridization betweenParkinsonia aculeata andCercidium praecox (Leguminosae:Caesalpinioideae) (1999)
    Springer
    Plant systematics and evolution 216 (1999), S. 49-68 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Parkinsonia aculeata ; Cercidium praecox ; Parkinsonia ×carterae ; Hybridization ; morphometric analysis ; cytology ; weeds
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Morphometric, cytogenetic, geographical and ecological evidence for hybridization betweenParkinsonia aculeata andCercidium praecox is presented. Morphometric investigation using the character count procedure and cytogenetic observations confirm hybrid status. All diagnostic morphometric characters were intermediate in the hybrid. Both parents (2n = 28) show regular tetrad formation and pollen fertility greater than 94%. Hybrids have a chromosome number of 2n = 28 or 2n = 30, and display meiotic abnormalities including lagging chromosomes and micronucleus formation; less than 21% of hybrid pollen was fertile. Ecological and geographical information suggests that hybridization is occurring at increasing frequency due to the expanding range ofP. aculeata associated with cultivation as an ornamental, coupled with ecological disturbance and weediness, and the cultivation ofC. praecox and hybrids as fodder, ornamental and shade trees. Hybrid fertility and phenological observations, in conjunction with F-weighted principal component analysis, suggest that the progeny of F1 hybrids are established. The hybrid is formally described asP. ×carterae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985100
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  • 29
    facet.materialart.
    Unbekannt
    Notes on Malesian Fabaceae (Leguminosae-Papilionoideae). 3. The genera Dioclea, Luzonia, and Macropsychanthus (1998)
    In:  Blumea - Biodiversity, Evolution and Biogeography of Plants (0006-5196) vol.43 (1998) nr.1 p.233
    Details
    Publikationsdatum: 2015-03-06
    Beschreibung: The genera Dioclea Humb., Bonpl. & Kunth, Luzonia Elmer, and Macropsychanthus Harms are briefly discussed. Keys to the species and notes to various taxa are given. Dioclea decandra Amshoff is proposed as a new name for Macropsychanthus ferrugineus Merr. Macropsychanthus lauterbachii Harms is lectotypified; its subspecies glabricalyx Verdc. and parviflorus Verdc. are lowered in rank to varieties, subsp. neobrittanicus Verde. is synonymized to var. parviflorus (Verdc.) Adema.
    Schlagwort(e): Leguminosae ; Dioclea ; Luzonia ; Macropsychanthus ; Malesia
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: Article / Letter to the editor
    Format: application/pdf
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  • 30
    facet.materialart.
    Unbekannt
    Notes on Malesian Fabaceae (Leguminosae-Papilionoideae). 3. The genera Dioclea, Luzonia, and Macropsychanthus (1998)
    In:  Blumea: Biodiversity, Evolution and Biogeography of Plants vol. 43 no. 1, pp. 233-239
    Details
    Publikationsdatum: 2024-01-12
    Beschreibung: The genera Dioclea Humb., Bonpl. & Kunth, Luzonia Elmer, and Macropsychanthus Harms are briefly discussed. Keys to the species and notes to various taxa are given. Dioclea decandra Amshoff is proposed as a new name for Macropsychanthus ferrugineus Merr. Macropsychanthus lauterbachii Harms is lectotypified; its subspecies glabricalyx Verdc. and parviflorus Verdc. are lowered in rank to varieties, subsp. neobrittanicus Verde. is synonymized to var. parviflorus (Verdc.) Adema.
    Schlagwort(e): Leguminosae ; Dioclea ; Luzonia ; Macropsychanthus ; Malesia
    Repository-Name: National Museum of Natural History, Netherlands
    Materialart: info:eu-repo/semantics/article
    Format: application/pdf
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  • 31
    Digitale Medien
    Digitale Medien
    Genetic differentiation of groundnut seed-beetle populations in Senegal (1998)
    Springer
    Entomologia experimentalis et applicata 87 (1998), S. 171-180 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Caryedon serratus ; Coleoptera ; Bruchidae ; genetic differentiation ; biotypes ; Senegal ; groundnut ; Caesalpinioideae ; Leguminosae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Caryedon serratus, the groundnut seed-beetle, is a major pest of groundnut (Arachis hypogaea), an introduced legume in the subfamily Papilionoideae. Native hosts of C. serratus in Senegal include Bauhinia rufescens, Cassia sieberiana, Piliostigma reticulatum and Tamarindus indica, all of which belong to the legume subfamily Caesalpinioideae. The biology and natural history of C. serratus suggest that it is a candidate for population differentiation via host-race formation. Evidence for host-tree associated differentiation in C. serratus would be important for the design of rational pest management practices. To test this possibility, we analyzed the genetic structure of 20 adult collections of C. serratus from six sites in Western Senegal, on its five hosts. Results show a strong differentiation of insects from different host trees, with specimens from C. sieberiana possibly representing a sibling species and insects from B. rufescens a distinct host-race.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003268331207
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  • 32
    Digitale Medien
    Digitale Medien
    Production of daidzein by callus cultures of Psoralea species and comparison with plants (1998)
    Springer
    Plant cell, tissue and organ culture 53 (1998), S. 35-40 
    Details
    ISSN: 1573-5044
    Schlagwort(e): callus culture ; daidzein ; flavonoid ; furanocoumarin ; Leguminosae ; secondary metabolite
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Callus cultures were established from five Psoralea species (Leguminosae) with the objective of producing daidzein (isoflavone). The biomass doubling times ranged from 7 to 16 days according to the species and a 48 weeks period was necessary to obtain lines with stable growth characteristics. All the 217 callus lines were analyzed for their daidzein content using HPLC. Our callus collection showed a large interspecific variation and the highest concentrations were recovered in P. obtusifolia callus lines (maximum of 0.9680% DW). Intraspecific variation was also important and allowed the recovery of high-producing lines (production exceeding 0.3000% DW) with four out of the five Psoralea species studied. The daidzein repartition was investigated in planta with P. cinerea in order to evaluate the potential of in vivo production. Mature fruits were the richest organs for daidzein concentration in P. cinerea and were used as indicators to evaluate the possible production with the other four plant species. In vitro concentrations were always much higher than in planta, and no correlation could be established between the calluses and plants for the five species. Our callus lines contained concentrations comparable to Psoralea hairy root lines. They can be considered as an interesting material to further study the production of daidzein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006057211490
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  • 33
    Digitale Medien
    Digitale Medien
    Isozyme diversity, RFLP of the rDNA and phylogenetic affinities among cultivated Lima beans,Phaseolus lunatus (Fabaceae) (1998)
    Springer
    Plant systematics and evolution 213 (1998), S. 153-164 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Phaseolus ; Isozymes ; genetic distance ; Lima bean ; rDNA ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Genetic variation inPhaseolus lunatus (Lima bean) was investigated at isozyme and DNA levels. Sixty cultivated accessions, including representatives of the Mesoamerican and Andean gene pools and intermediate types, were analyzed for variability at 17 isozyme loci. Some accessions were also examined for restriction fragment length polymorphism (RFLP) at the rDNA level. These data were used to construct two dendrograms showing clear separation in two distinct groups corresponding to each of the gene pools and an intermediate one probably representing a transitional group.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985196
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  • 34
    Digitale Medien
    Digitale Medien
    A second spontaneous hybrid in the genusLeucaena (Leguminosae, Mimosoideae) (1998)
    Springer
    Plant systematics and evolution 212 (1998), S. 53-77 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Leucaena leucocephala ; L. diversifolia ; L. ×spontanea ; Hybridization ; hybrid detection ; spontaneous hybrid ; RAPD ; RFLP ; chloroplast DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The detection of hybridity inLeucaena is discussed in relation to: (i) traditional criteria, (ii) molecular criteria and (iii) models to predict hybrid leaf morphology. Morphological, geographical and molecular evidence for the occurrence of interspecific hybrids betweenL. leucocephala andL. diversifolia in south-central Mexico, northern Guatemala, Jamaica, Dominican Republic, the Philippines and Papua New Guinea is presented. Predicted mean hybrid leaf trait values calculated from parent material are compared with data from putative hybrids and shown to be similar. The origin of these hybrids is discussed and shown to be the result of artificial sympatry resulting from indigenous, and recent exotic, domestication of the parent species. The hybrid is described asL. ×spontanea.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985221
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  • 35
    Digitale Medien
    Digitale Medien
    Fire-type and forestry management effects on the early postfire vegetation dynamics of a Pinus pinaster woodland (1998)
    Springer
    Plant ecology 134 (1998), S. 27-41 
    Details
    ISSN: 1573-5052
    Schlagwort(e): Cistus ladanifer ; Crown-fire ; Fire severity ; Leguminosae ; Mediterranean-type ecosystems ; Spain ; Surface-fire
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The objective of this research was to study the effects of type of fire, prefire-, and postfire-management on the postfire vegetation dynamics of a Pinus pinaster woodland in Central Spain, burned at 15 yr of age. The effects of type of fire (crown-, or surface-fire), prefire-management (thinning out of trees and clearing of brush or no such actions) and postfire-management (removal of burned trees one year after the fire or no such action) on the postfire vegetation were studied during the first three years after the fire. Herbaceous plant abundance, species richness, and diversity, as well as abundance, growth and density of the dominant shrub species (Cistus ladanifer) were measured during the first three years after the fire. Our results show that the effects of the type of fire on the vegetation were minimal. Prefire-management effects were significant on the abundance of herbaceous species, mainly during the second and third year after fire, in particular for the Leguminosae species. Prefire managed areas were more diverse in species, and produced higher plant biomass than unmanaged areas. Postfire-management effects on the shrubs and herbs were minimal, except for the Leguminosae, which increased their cover where the trees had been removed. Plant dynamics were marked by the interaction between prefire-management and fire-type through the dynamics of the shrub cover. On most occasions, plots that resulted in lower cover of C. ladanifer had greater abundance of herbaceous plants and, in particular, of the Leguminosae. In general, our results show that irrespective of fire-type, prefire-, or postfire-management all areas tended to be very similar in their vegetation three years after the fire.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009733818670
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  • 36
    Digitale Medien
    Digitale Medien
    Influence of heat on seed germination of seven Mediterranean Leguminosae species (1998)
    Springer
    Plant ecology 136 (1998), S. 95-103 
    Details
    ISSN: 1573-5052
    Schlagwort(e): Hardseedness ; Heat ; Leguminosae ; Seed germination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The influence of high temperatures (dry heat and hot water) on germination of seven Mediterranean Leguminosae species typical of fire-prone ecosystems in southern Spain is analyzed, in order to know the response of seeds to wildfires and the possible implications in their regeneration after this disturbance. Seeds were heated to a range of temperatures (50 °–150 °C) and exposure times (1–60 min) similar to those registered in the upper soil layers during wildfires. Germination tests were carried out in plastic Petri dishes over 60 days. In general, the degree of seed germination promotion by dry heat treatments showed a wide interspecific variation, although the final germination level was increased in all the studied species except for Scorpiurus muricatus. The thermal pretreatment of 50 °C, however, was not effective for germination in any species, and rising the temperature to 70 °C only slightly enhanced the germination in Cytisus patens. The preheatings of 90 °C (5 and 10 min), 120 °C (5 and 10 min), and 150 °C (1 min) were the most effective in promoting seed germination. Hot water (100 °C) scarification also increased the final germination level in all cases, with the exception of C. patens. The germination rates after preheating were much lower than in mechanically scarified seeds and closely resembled those of the untreated seeds, except for C. reverchonii, whose seed germination rate decreased with heat. The response of species to heat shock had no clear relationship with life trait or with the specific post-fire regeneration strategy (obligate seeder or facultative resprouter). Those species coexisting in the same habitats had different heat optimal requirements for seed germination, an strategy suggested by some authors as minimizing interspecific competition in the secondary succession started after fire.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009702318641
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  • 37
    Digitale Medien
    Digitale Medien
    Chemotaxonomic features of lipophilic components inAdesmia species (Leguminosae) (1998)
    Springer
    Plant systematics and evolution 210 (1998), S. 141-145 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Adesmia grandiflora ; A. retrofracta ; A. incana ; A. bicolor ; A. trijuga ; A. horrida ; Leguminosae ; Chemical composition ; chemotaxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Studies were performed on GC-MS to assess the lipophilic composition of sixAdesmia species representing two subgenera and three series. Normal fatty acids and hydrocarbons were mainly found, as well as acetylenic compounds, dibasic acids, cyclic hydrocarbons, high molecular weight alcohols and one sterol.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984732
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  • 38
    Digitale Medien
    Digitale Medien
    Regeneration of plants from callus tissue of Desmodium affine and Desmodium uncinatum (1997)
    Springer
    Biologia plantarum 39 (1997), S. 309-313 
    Details
    ISSN: 1573-8264
    Schlagwort(e): Leguminosae ; growth regulators ; tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Plants were in vitro regenerated from leaf callus of Desmodium affine and D. uncinatum. Leaf explants were induced to form callus when aseptically cultured on Murashige and Skoog medium (MS) supplemented with 6 mg dm-3 6-benzylaminopurine (BAP) in combination with 1 mg dm-3 naphthaleneacetic acid (NAA). Regeneration of shoots was induced when callus was cultured on MS medium supplemented with 6 mg dm-3 BAP and 0.01 mg dm-3 NAA. Roots regenerated in high frequency when differentiated shoots were subcultured on MS medium supplemented only with 0.01 mg dm-3 NAA. The regenerated plantlets were successfully grown in pots. Calli from D. incanum failed to regenerate shoots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1000696319639
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  • 39
    Digitale Medien
    Digitale Medien
    Oviposition Stimulants for Sulfur ButterflyColias erate poliographys: Cyanoglucosides as Synergistis Involved in Host Preference (1997)
    Springer
    Journal of chemical ecology 23 (1997), S. 323-331 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Oviposition stimulants ; Colias erate ; Lepidoptera ; Pieridae ; Trifolium repens ; Leguminosae ; cyanoglucosides ; linamarin ; lotaustralin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Host-plant chemicals stimulating oviposition by a Leguminosae-feeding pierid butterflyColias erate poliographyswere isolated and identified from one of its primary host plants, white clover (Trifolium repens). Females readily deposited eggs in response to methanolic extracts of the plant, and subsequent partition of the extracts with organic solvents revealed that chemical constituents critical for host recognition reside in the water-soluble fraction. Further fractionation of the hydrosoluble fraction by column chromatography led to the separation of an active fraction and two cyanoglucosides, linamarin and lotaustralin. Conspicuous oviposition response was evoked by unidentified polar compound(s), while these cyanoglucosides exerted no stimulatory activity by themselves. However, ovipositing females preferred samples containing either of the two cyanoglucosides. In dual-choice bioassays, significantly more eggs were laid on samples admixed with the cyanoglucosides, suggesting that the cyanoglucosides serve as synergistic oviposition stimulants and could play an important role in host selection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/B:JOEC.0000006362.96722.c9
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  • 40
    Digitale Medien
    Digitale Medien
    Distribution of allozyme variation inVicia benghalensis (Leguminosae) suggests the existence of two genepools (1997)
    Springer
    Plant systematics and evolution 207 (1997), S. 99-110 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Vicia benghalensis ; Genetic diversity ; genetic structure ; isozymes ; reproductive system
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract InVicia benghalensis two biotypes differing in biochemical and cytological features were identified in previous studies. It was hypothesized that these biotypes could also vary in reproductive behaviour and be genetically isolated. To better understand this issue, 25 distinct samples were studied using allozymes. Nine enzyme systems, accounting for 15 putative loci, were scored. The data obtained were analysed using population diversity parameters; Nei's genetic distance was calculated and used to construct a dendrogram. Principal component analysis was conducted to verify the dendrogram and to determine which alleles had stronger influence on the grouping of populations. The analyses demonstrated that the two previously identified biotypes are genetically different: Nei's distance between the two groups is larger than the distance within each group. Three-dimensional plotting of the first three principal components corroborates this division. Genetic diversity parameters confirm that the two biotypes also differ in outcrossing rates. These results denote the existence of two highly divergent genepools inV. benghalensis and suggest that a speciation process may be acting. The consequence of the present findings on the management of gene bank collections is analysed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985211
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  • 41
    Digitale Medien
    Digitale Medien
    Isozyme variation inFaidherbia albida (Leguminosae, Mimosoideae) (1997)
    Springer
    Plant systematics and evolution 207 (1997), S. 119-132 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Faidherbia albida ; Acacia albida ; Isozyme variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Genetic variation has been assessed in 30 populations (931 families) ofFaidherbia albida (Leguminosae, Mimosoideae) from across its entire African range, using six isozyme loci identified by five enzyme systems. Among the populations studied a null allele was proposed to explain the absence ofLap-1 activity in populations from southern and eastern Africa. The mean percentage of polymorphic loci per population, the mean number of alleles per locus and the mean genetic diversity within populations were 31.7%, 1.6 and 0.127 respectively. Genetic diversity was greatest in populations from West Africa and lowest in populations from eastern/southern Africa, with Ethiopian/Sudanese populations intermediate. The overall degree of genetic differentiation between populations (GST) indicated that approximately 56% of the enzyme variation resided within populations. Clustering of Nei's unbaised genetic distances calculated between all populations produced a dendrogram that generally followed the geographic distribution of the populations. Two major groups were identified that may be considered the eastern/southern African and the Ethiopian/West African clusters. Within the Ethiopian/West African cluster two subclusters could be recognised, one broadly corresponding to those populations from Ethiopia/Sudan and the other to those populations from West Africa. The implications of these results for theories regarding the origin ofF. albida in Africa are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985213
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  • 42
    Digitale Medien
    Digitale Medien
    Genetic analysis among and within populations forming ecotypes and cultivars of lucerne,Medicago sativa (Leguminosae), using RAPD fragments (1997)
    Springer
    Plant systematics and evolution 208 (1997), S. 107-119 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Medicago sativa ; Alfalfa ; cultivar discrimination ; DNA ; RAPD ; population genetics ; Mahalanobis distance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We conducted this study to determine whether the analysis of the population frequency of individual RAPD fragments amplified from DNA of single seedlings could be used to discriminate cultivars of lucerne and compare the relatedness of cultivars of differing geographic and genetic origin. Canonical discriminant analysis of RAPD banding data obtained from amplification of DNA from 40 seedlings of each of ten cultivars (six Iranian ecotypic cultivars, two cultivars from New Zealand and two from the USA) with one selected primer enabled discrimination of most cultivars (78% of pairwise comparisons at P 〈 0.01 among the ten cultivars examined in this study). Comparison of the Mahalanobis generalized distances among the cultivars produced results for genetic relatedness which were generally consistent with geographical origin and breeding relationships. Cultivar uniformity was assessed by determining the percentage of seedlings correctly classified into the named cultivar; this was variable among cultivars and the Iranian ecotypic cultivars had a particularly wide range of variability. Analysis of population genetics in lucerne using RAPD banding data may be useful for plant improvement, in descriptions of new cultivars and also when assessing cultivar purity in seed certification programmes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00986085
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  • 43
    Digitale Medien
    Digitale Medien
    Phenetic investigation of non-nodulating African species ofAcacia (Leguminosae) using morphological and molecular markers (1997)
    Springer
    Plant systematics and evolution 205 (1997), S. 27-51 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Mimosoideae ; Acacia ; Aculeiferum ; Monacanthea ; African acacias ; classification ; molecular markers ; RAPD ; morphology ; nodulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Morphological and RAPD markers were used to assess the relationships among nodulating and non-nodulating species of AfricanAcacia. Non-nodulating species of AfricanAcacia are only found within subg.Aculeiferum sect.Monacanthea. African species of sect.Monacanthea examined were found to form a group distinct from the other African species examined on a morphological and molecular basis. All lack the ability to nodulate, suggesting that non-nodulation may be used as a taxonomic tool. The species of sect.Aculeiferum were separated by RAPD and morphological analysis into two groups depending on whether they were armed with prickles in pairs and/or prickles in threes, or solitary. A third group of species was identified within sect.Acacia: further subdivision of this group was achieved into subsectt.Pluriseriae andUniseriae. The position ofA. albida relative to other AfricanAcacia species was found to be distinct but not totally independent of the genus. The partitioning and distribution of the genetic variability within the genus is further elucidated by the RAPD analysis of populations ofAcacia species. A population analysis ofA. polyacantha demonstrated geographical and site-specific variation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00982796
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  • 44
    Digitale Medien
    Digitale Medien
    Molecular phylogeny and phylogeography ofLupinus (Leguminosae) inferred from nucleotide sequences of therbcL gene and ITS 1 + 2 regions of rDNA (1997)
    Springer
    Plant systematics and evolution 208 (1997), S. 139-167 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Papilionoideae ; Genisteae ; Lupinus ; Molecular phylogeny ; cpDNA ; rbcL ; rDNA ; internal transcribed spacer (ITS)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Total DNA was extracted from 55 species of theLeguminosae (including 29 species ofLupinus). The chloroplast generbcL and the ITS 1 + 2 regions of nuclear RNA genes were amplified by polymerase chain reaction (PCR) and sequenced directly. The sequences obtained were evaluated with character state (Maximum Parsimony) and distance methods (Neighbour Joining). Phylogenetic trees obtained with both data sets and methods are mostly congruent.Genisteae andCrotalarieae are sister groups and share ancestry with theThermopsideae/Podalyrieae. The genusLupinus, which forms a monophyletic clade within theGenisteae, shows a distinct Old-New World disjunction and appears to be divided into several more or less distinct groups: (1) The species from the eastern part of South America. (2) The homogeneous rough-seeded group (Scabrispermae) of the Old World species which is well distinguished from the smooth-seeded group (Malacospermae). (3) Within the rather heterogeneous smooth-seeded lupins a smaller subgroup withL. angustifolius, L. hispanicus andL. luteus is recognized. (4) Also separated are North American lupins and South American species with a western distribution. Genetic distances imply that the genusLupinus evolved during the last 12–14 million years, ruling out the hypothesis that the present Old-New World disjunction can be interpreted as a result of the continental drift. The genetic data suggest an origin in the Old World and an independant colonisation of the Eastern parts of South America as opposed to North America and the Western parts of South America.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985439
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  • 45
    Digitale Medien
    Digitale Medien
    Characterisation of a developmentally related polypeptide with glutelin solubility characteristics from Lupinus albus L. (1996)
    Springer
    Planta 198 (1996), S. 221-229 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Germination ; Glutelin ; Glycoprotein ; Leguminosae ; Lupinus ; Seed development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Proteins from Lupinus albus L. cv. Rio Maior seeds were fractionated according to solubility criteria. Patterns of concanavalin A (ConA)-binding polypeptides from the different classes, albumins, globulins, glutelins and prolamins, were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Two bands of apparent molecular masses of 29 and 23.5 kDa with glutelin solubility characteristics bound the lectin. The 23.5-kDa band was separated by two-dimensional electrophoresis into two components: one glycosylated and heterogeneous with an isoelectric point of approx. 10 (designated as G23) and another, not detected with ConA, precipitating in the first dimension. The amino acid and hexosamine analysis of G23 showed that it is particularly rich in Gly (11.2%), Glx (10.0%), Ser (9.0%), Leu (8.2%), Asx (7.5%), and Pro (6.7%) and that it has a considerable content of the sulphur-containing amino acids Met (2.0%) and Cys (5.8%) and contains glucosamine. The determined N-terminal amino acid sequence of G23 was: 1KG(R)V5KGTGD10(T)PXXV15XLY(N)R20T, and this had no significant similarity to any of the amino acid sequences contained in the data bank SWISS-PROT 26. The glycoprotein G23 was completely deglycosylated with peptide-N-glycosidase F, yielding a homogeneous 21-kDa polypeptide composed of approximately 191 amino acids. The structures of the major N-linked neutral oligosaccharides of G23, determined by exoglycosidase sequencing, were as follows: Manα2Manα6(Manα3) Manα6(Manα2Manα2Manα3)Manβ4GlcNAcβ4GlcNAc (13%); ± Manα2Manα6(Manα3)Manα6(± Manα2 Manα2 Manα3)Manβ4GlcNAcβ4GlcNAc (29%); Manα6(Manα3) Manα6(Manα2Manα3)Manβ4GlcNAcβ4GlcNAc (13%); Manα6(Manα3)Manα6(Manα3)Manβ4GlcNAcβ4GlcNAc (16%); Manα6(Manα3)(Xylβ2)Manβ4GlcNAc β4GlcNAc (28%). Changes in G23 abundance during seed development, germination and seedling growth were monitored with a specific antibody. The glycoprotein G23 started to accumulate appreciably during seed formation between the 40th and the 50th days after anthesis and was detected following seed imbibition, until the 9th day in cotyledons, the 2nd day in roots and the 4th day in hypocotyls and leaves.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00206247
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  • 46
    Digitale Medien
    Digitale Medien
    Symbiotic specialization and the potential for genotypic coexistence in a plant-bacterial mutualism (1996)
    Springer
    Oecologia 108 (1996), S. 361-367 
    Details
    ISSN: 1432-1939
    Schlagwort(e): Bradyrhizobium ; Competition ; Genetic diversity ; Leguminosae ; Mutualism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Genotypes of the annual legume Amphicarpaea bracteata vary in their degree of specialization toward different nitrogen-fixing bacteria. Plants of lineages “Ib” and “II” are specialized for mutualism with a limited group of bacterial genotypes. Lineage “Ia” plants are symbiotic generalists, interacting with all bacteria associated with specialist (Ib and II) plants, and also with a distinct class of bacteria that only fix nitrogen with lineage Ia plants. The relative performance of generalist and specialist plant lineages was measured in four symbiotic environments: (1) in the absence of nitrogen-fixing bacteria, (2) with bacteria with broad host ranges, (3) with bacteria specialized on lineage Ia hosts, and (4) with a mixture of the bacteria with broad and narrow host ranges. In the presence of bacteria with broad host ranges, the relative performance of different plant lineages was inconsistent among experimental replicates. However, lineage Ia plants had nearly 3 times higher total biomass and 6 times higher seed biomass than lineage Ib or II plants when grown with bacteria specialized on Ia hosts. When exposed to a mixture of bacteria with broad and narrow host ranges, generalist plants had 72% higher total biomass and 〉100% higher seed biomass than specialist plants. These results imply that in diverse natural populations, where all plants have a choice of symbiotic partners, mutualistic interactions are likely to foster competitive exclusion rather than stable coexistence of different plant lineages.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00334662
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  • 47
    Digitale Medien
    Digitale Medien
    Identification of sex pheromones from cowpea weevil,Callosobruchus maculatus, and related studies withC. analis (coleoptera: Bruchidae) (1996)
    Springer
    Journal of chemical ecology 22 (1996), S. 2233-2249 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Carboxylic acids ; Leguminosae ; pulses ; stored products ; seed weevils ; Callosobruchus maculatus ; Coleoptera ; cowpea weevil
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Female cowpea weevils,Callosobruchus maculatus, produce a sex pheromone that elicits orientation and sexual behavior in males. Bioassay-directed isolation of the sex pheromone was conducted and compounds in the active fraction were identified and synthesized. Volatiles were collected from individual virgin females by adsorption on filter paper dises and hexane extraction. A bioassay was used in which the locomotory response of single males in glass vials was recorded upon exposure to treatments or controls. Crude extracts were subjected to silica gel column chromatography with solvents of increasing polarity; all activity eluted with methanol. Activity in the highly polar methanol fraction suggested a carboxylic acid or a compound with multiple polar functionality. Acid-base partitioning of the crude extract isolated all activity in the acid fraction, confirming that the pheromone was a carboxylic acid. The acid fraction was further fractionated by preparative GC with a Carbowax column. The most active GC fraction contained the following five 8-carbon acids identified by GC-MS and comparison with synthetic candidates: 3-methyleneheptanoic acid, (Z)-3-methyl-3-heptenoic acid, (E)-3-methyl-3-heptenoic acid, (Z)-3-methyl-2-heptenoic acid, and (E)-3-methyl-2-heptenoic acid. Each of the synthetic acids was active individually for males, and combinations of two or more of the acid pheromones had an additive effect. Upwind flight responses to natural and synthetic pheromones were observed in a flight tunnel. (Z)-3-Methyl-2-heptenoic acid was previously identified as the sex pheromone for the relatedC. analis, but this and the other four acid pheromones fromC. maculatus were inactive for maleC. analis. There was no cross-attraction betweenC. maculatus andC. analis in reciprocal studies using extracted volatiles from females of both species, GC-MS analysis ofC. analis female volatiles failed to detect any of theC. maculatus compounds but did find an unidentified C-8 acid with a GC retention time different from any of theC. maculatus pheromones.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02029543
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  • 48
    Digitale Medien
    Digitale Medien
    Compensatory growth response of the legume, Medicago sativa, to defoliation and denodulation (1996)
    Springer
    Entomologia experimentalis et applicata 78 (1996), S. 243-252 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Leguminosae ; denodulation ; defoliation ; compensatory growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A laboratory study was conducted to determine the effects of defoliation and denodulation on compensatory growth of Medicago sativa (L.). Plants grown hydroponically in clear plastic growth pouches were subjected to 0 and 50% nodule pruning, and 0, 25, 50, and 75% defoliation by clipping trifoliate leaves. An additional experiment was conducted to determine if clipping leaves simulated herbivory by Hypera postica (Gyllenhal) larvae. Previously, we determined that nodule pruning accurately simulated herbivory by Sitona hispidulus (L.) larvae (Quinn & Hall, 1992). Results indicated that denodulation stimulated nodule growth and caused exact compensation in standing and total number of nodules per plant within 15 days and in standing nodule biomass within 22 days of treatment. Denodulation caused a significant reduction (13%) in final shoot biomass, but did not affect significantly final root biomass. Percentage of change in number of trifoliate leaves per plant increased with the level of defoliation. Within 22 days of treatment, total number of trifoliate leaves per plant was similar to controls. However, final standing shoot biomasses were significantly less that controls, indicating undercompensatory growth. Shoot biomasses of the 25-, 50-, and 75%-defoliated plants were 18, 20, and 36% lower than controls, respectively. Nodule biomass per plant was reduced by 24 and 32% in 50- and 75%-defoliated plants, respectively, but was not affected significantly by 25% defoliation. Root biomass was affected by all levels of defoliation. Clipping trifoliate leaves accurately simulated defoliation by H. postica larvae. Our results indicated that partial defoliation affected shoot, root, and nodule biomass of M. sativa, but that partial denodulation only affected shoot biomass.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00193443
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  • 49
    Digitale Medien
    Digitale Medien
    Niche relations in Vicia-inhabiting Bruchus spp. (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 152-155 
    Details
    ISSN: 1570-7458
    Schlagwort(e): predispersal seed predator guild ; Leguminosae ; Bruchidae ; Curculionidae ; Lepidoptera ; intra-and interspecific competition ; Monte Carlo simulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00194746
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  • 50
    Digitale Medien
    Digitale Medien
    Adaptation of a Callosobruchus maculatus strain to Vicia faba, as its new host plant (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 156-159 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Bruchidae ; glucosides ; Leguminosae ; larval development ; host-plant adaptation ; β-glucosidase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00194747
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  • 51
    Digitale Medien
    Digitale Medien
    Sequence of a new Bowman-Birk inhibitor fromTorresea acreana seeds and comparison withTorresea cearensis trypsin inhibitor (TcTI2) (1996)
    Springer
    The protein journal 15 (1996), S. 553-560 
    Details
    ISSN: 1573-4943
    Schlagwort(e): Leguminosae ; protein sequencing ; Torresea acreana, serine proteinase inhibitor, Bowman-Birk inhibitor
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract TaTI (Torresea acreana trypsin inhibitor), a new member of the Bowman-Birk trypsin inhibitor family, was purified from seeds ofTorresea acreana, one of the two known species ofTorresea, a Brazilian native Leguminosae of the Papilionoideae subfamily. Purification was performed by acetone fractionation, anion-exchange chromatography, and gel filtration. The TaTI appears asM r 7000 in SDS-PAGE under reducing conditions. There are 63 amino acid residues present in the TaTI sequence, which was confirmed by mass spectrometry (8388 daltons). The putative reactive sites residues were Lys-15 and Arg-42 at the first and second site, respectively. The antibodies raised against TcTI2,Torresea cearensis trypsin inhibitor 2, showed a cross-reaction with TaTI, but not with other Bowman-Birk inhibitors purified from Leguminosae. The inhibition constants of TaTI and TcTI2 were comparable when measured against trypsin, chymotrypsin, and factor XIIa, but not on plasmin. The latter was tenfold more effectively inhibited by TcTI2 then by TaTI. Neither TaTI nor TcTI2 affects thrombin, plasma kallikrein, or factor Xa.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01908537
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  • 52
    Digitale Medien
    Digitale Medien
    Survey of leaf anatomy, especially secretory structures, of tribeCaesalpinieae (Leguminosae, Caesalpinioideae) (1996)
    Springer
    Plant systematics and evolution 200 (1996), S. 21-39 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Caesalpinioideae ; Cavities ; glands ; idioblasts ; leaf anatomy ; stomata ; trichomes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We studied leaflet anatomy, emphasizing secretory structures, from herbarium specimens of 128 species of 44 genera of tribeCaesalpinieae, using clearings, resin sections, and scanning electron microscopy. These observations, combined with those from our three earlier papers, provide a survey of 210 species representing all genera. Seventy-three species had secretory structures: 21 had glands or gland-like trichomes, 40 had living mesophyll idioblasts, and nine had cavities (three species each had two different types). Five additional species, all inCercidium (Caesalpinia group), had paired or clustered large spheroidal, thick-walled, empty cells (veinlet idioblasts) interconnected by perforation plate-like gaps. Secretory structures have systematic significance at various taxonomic levels.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984746
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  • 53
    Digitale Medien
    Digitale Medien
    Genista valsecchiae (Leguminosae), a new species from Sardinia (1996)
    Springer
    Plant systematics and evolution 200 (1996), S. 273-279 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Genista ; Taxonomy ; Flora of Sardinia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Genista valsecchiae from SW Sardinia is described and illustrated as a species new to science. It is very similar toG. ephedroides and can be distinguished by morphological differences of habit and inflorescence. It typically occurs in the garigue or maquis and is widespread in SW Sardinia.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984941
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  • 54
    Digitale Medien
    Digitale Medien
    Phylogenetic relationships in the genusStylosanthes (Leguminosae) based upon chloroplast DNA variation (1996)
    Springer
    Plant systematics and evolution 200 (1996), S. 193-211 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Stylosanthes ; Chloroplast DNA ; RFLPs ; phylogeny ; systematics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A detailed analysis of chloroplast DNA restriction fragment length variation was undertaken to reconstruct the maternal phylogeny of 18 taxa from both sections of the papilionoid tropical forage legume genusStylosanthes. Data were analysed by means of the computer program PAUP, using an heuristic search with Wagner parsimony. The resulting cladogram dividedStylosanthes into four separate clades, which comprised: (i) theS. guianensis complex and related species (i.e.S. gracilis, S. grandifolia andS. montevidensis); (ii)S. hispida, tetraploidS. hamata s. l.,S. sympodialis, S. humilis, S. leiocarpa, S. angustifolia and certain accesions ofS. scabra; (iii)S. calcicola, S. viscosa, diploidS. hamata s. str., andS. fruticosa, plus accessions ofS. scabra, S. capitata and one accession ofS. grandifolia; and (iv)S. macrocephala and other accessions ofS. capitata not included within clade 3. Results are generally congruent with previously established interspecific relationships and, moreover, enabled identification of putative maternal progenitors for four tetraploid taxa:S. humilis was identified as a likely maternal parent of bothS. sympodialis andS. hamata s. l.,S. viscosa as a maternal parent ofS. scabra, andS. macrocephala as a maternal parent ofS. capitata.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984935
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  • 55
    Digitale Medien
    Digitale Medien
    Functional reconstitution of ion channels from Paramecium cortex into artificial liposomes (1995)
    Springer
    The journal of membrane biology 144 (1995), S. 199-208 
    Details
    ISSN: 1432-1424
    Schlagwort(e): Channel reconstitution ; Ca2+-release channel ; Paramecium ; Liposome ; Internal membrane
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Toward isolating channel proteins from Paramecium, we have explored the possibility of functionally reconstituting ion channels in an artificial system. Proteins from Paramecium cortex reconstituted with soybean azolectin retained several channels whose activities were readily registered under patch clamp. The most commonly encountered activities were three: (i) a 71-pS cation channel that opens at all voltages unless dior trivalent cations were added to close them, (ii) a 40 pS monovalent cation channel, and (iii) a large-conductance channel that prefers anions and exhibits many subconductance states. These channels survived mild detergent treatments without observable functional alterations. The possible origin of these channels from internal membranes, the possible role of 71-pS channel in internal Ca2+ release, and the prospects of their purification are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00236833
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  • 56
    Digitale Medien
    Digitale Medien
    Pea leaf weevil herbivory on pea seedlings: effects on growth response and yield (1995)
    Springer
    Entomologia experimentalis et applicata 76 (1995), S. 255-269 
    Details
    ISSN: 1570-7458
    Schlagwort(e): herbivory ; Curculionidae ; Sitona lineatus (L.) ; Leguminosae ; Pisum sativum L. ; compensatory growth ; yield
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Field experiments were conducted to determine growth and yield responses ofPisum sativum L. to defoliation by adultSitona lineatus (L.). Seedlings grown under conventional (moldboard plowed) and conservation (chisel plowed) tillage treatments were infested for a 1-week period with 0, 1 and 8 weevils per plant at two times: at 75% field emergence and 1 week later. After the early infestation, defoliation for the control, low and high weevil densities was about 0,15 and 50%, respectively, while defoliation after the late infestation was about 0, 10 and 35%. An undercompensatory growth response was observed in one experiment after seedlings were subjected to moderate levels of early defoliation. Exact compensation was observed in two experiments after early infestations of low and highSitona densities.Sitona defoliation reduced the number of pods per plant and pod length in two experiments. However, seed biomass was never significantly reduced. Averaged over all experiments, reduction in seed biomass due to highSitona densities was 10 and 5% for early and late infestations, respectively. Tillage treatments did not affectPisum compensatory growth response, although yield components were sometimes greater in conservation tillage than in conventional tillage, possibly due to slightly greater soil moisture in the conservation tillage plots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02382992
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  • 57
    Digitale Medien
    Digitale Medien
    Relationship between physicochemical properties and chemical stability of muramyldipeptide derivative B30-MDP in liposomal solutions (1995)
    Springer
    Colloid & polymer science 273 (1995), S. 399-404 
    Details
    ISSN: 1435-1536
    Schlagwort(e): B30-MDP ; Fluidity ; Liposome ; Octyl-β-D-glucoside ; Stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Maschinenbau
    Notizen: Abstract The muramyldipeptide derivative B30-MDP has immunoadjuvant activity and vesicle-forming ability in aqueous environments. It is therefore important to evaluate the relationship between its physicochemical properties and chemical stability for use as a vaccine adjuvant. We studied the effects of octyl-β-D-glucoside (O.G.) incorporation on the physicochemical properties and chemical stability in aqueous solution at pH 7.4. The changes in particle size and in the membrane fluidity of B30-MDP liposomes, which were induced by the addition of O.G., were measured to confirm the transition from micelle phase to vesicle phase. The degradation of B30-MDP in both liposomal and mixed micellar solutions was measured by reverse-phase high-performance liquid chromatography. This degradation occurred by a pseudo first-order reaction at 313, 323 and 333 K. The shelf-life of the B30-MDP solution supplemented with O.G. was approximately one-seventh of that of B30-MDP alone in the liposomal solution. The changes in thek obs values of B30-MDP correlated well with those in membrane fluidity induced by O.G. incorporation. These results indicate that an increase in membrane fluidity labilizes B30-MDP in liposomal solution.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00652356
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  • 58
    Digitale Medien
    Digitale Medien
    Antitermitic activity ofLonchocarpus castilloi flavonoids and heartwood extracts (1995)
    Springer
    Journal of chemical ecology 21 (1995), S. 455-463 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Feeding deterrents ; termites ; Cryptotermes brevis ; Isoptera ; Lonchocarpus castilloi ; Leguminosae ; heartwood ; flavonoids ; antitermitic activity ; castillen D ; castillen E
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The heartwood of the tropical treeLonchocarpus castilloi Standley (Leguminosae) is highly resistant to attack by the drywood termitesCryptotermes brevis (Walker); nevertheless successive extraction with hexane, diethyl ether, acetone, methanol, and water reduced its resistance to these organisms. Antitermitic properties of the extracts were bioassayed using impregnated filter paper disks. Although the five extracts reduced both feeding and survival ofC. brevis, no significant differences among them were detected. Choice feeding tests showed that termites avoided eating the paper treated with the extracts. Two flavonoid compounds isolated from the heartwood, castillen D and castillen E, impregnated into filter paper showed concentration-dependent feeding deterrent activity, but were not toxic toC. brevis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02036742
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  • 59
    Digitale Medien
    Digitale Medien
    Influence of two successive learning processes on the response ofEupelmus vuilleti Crw (Hymenoptera: Eupelmidae) to volatile stimuli from hosts and host plants (1995)
    Springer
    Journal of insect behavior 8 (1995), S. 751-762 
    Details
    ISSN: 1572-8889
    Schlagwort(e): Eupelmidae ; Bruchidae ; Leguminosae ; ectoparasitoid ; host-searching ; preemergence learning ; ovipositional experience
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Eupelmus vuilleti (Crw) is a solitary ectoparasitoid of several bruchid species which develop inside Leguminosae seeds. In this hymenopteran a preemergence learning process is responsible for a specific response of females toward the host and host-plant volatiles. This learning occurs after the imaginal molt, while the adult remains in the larval chamber of its host. The persistence of the response toward host and host-plant volatiles of females reared onBruchidius atrolineatus (Pic) larvae developing inVigna unguiculata (Walp) seed was investigated in this study. In the absence of any reinforcement, the response towardB. atrolineatus larvae persisted for 6 days after female emergence from the seeds, whereas the response towardV. unguiculata seed volatiles was maintained for 7 days. Our experiments showed that a later learning process also takes place inE. vuilleti. An ovipositional experience on a given plant-host complex enabled females to learn the stimuli from that complex. This learning occurred even if the complex experienced during oviposition differed from the one on which females were reared. After an ovipositional experience on a new plant-host complex, the response acquired through preemergence learning was maintained. These experiments demonstrate that theE. vuilleti females can memorize cues from several species during successive learning processes. The adaptive significance of these learning capacities, which confer an important behavioral plasticity to this generalist parasitoid, is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02009504
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  • 60
    Digitale Medien
    Digitale Medien
    Systematics and randomly amplified polymorphic DNA in the genusLeucaena (Leguminosae, Mimosoideae) (1995)
    Springer
    Plant systematics and evolution 197 (1995), S. 195-208 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Leucaena ; RAPDs ; systematics ; cpDNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Randomly amplified polymorphic DNA (RAPD) was used to examine genomic diversity in taxa of the neotropical legume genusLeucaena. Data were analysed using both similarity- and parsimony-based approaches and the data compared to a parsimonybased analysis of chloroplast DNA restriction fragment length polymorphisms (RFLP). Distance-based methods of RAPD analysis produced groups inconsistent with those identified by RFLP analysis. Parsimony-based analysis of the data produced groupings largely consistent with those identified using RFLPs. The major differences were grouping of the two subspecies ofLeucaena diversifolia (subsp.diversifolia and subsp.stenocarpa) in the RAPD tree, but their separation in the RFLP tree. The value of RAPD data in systematics as a result of these data and our understanding of the molecular basis of RAPDs are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984639
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  • 61
    Digitale Medien
    Digitale Medien
    Microgametophytic plastid nucleoid content and reproductive and life history traits of tribeTrifolieae (Fabaceae) (1995)
    Springer
    Plant systematics and evolution 196 (1995), S. 89-98 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Medicago ; alfalfa ; Microgametophyte ; biparental inheritance ; DAPI
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Microgametophytic plastid nucleoids were quantified for 18 species representing the four core genera of the tribeTrifolieae (Fabaceae),Medicago, Melilotus, Trigonella, andTrifolium. Generative cells of all taxa contained nucleoids, establishing that biparental plastid inheritance is common in theTrifolieae. Nucleoid number and volumes of pollen grains and generative cell nuclei differed among taxa. Nucleoid number was positively correlated with pollen grain and generative cell nuclear volumes, flower size and style length. These relationships disappeared after adjusting nucleoid number for pollen grain and generative cell nuclear volumes. Adjusted nucleoid numbers provided no evidence to support hypotheses that plastid content is associated with ploidy level, mating system, perenniality or size of the reproductive apparatus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985337
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  • 62
    Digitale Medien
    Digitale Medien
    The morphology of styles and stigmas inVicia (Leguminosae), and its systematic implications (1995)
    Springer
    Journal of plant research 108 (1995), S. 17-24 
    Details
    ISSN: 1618-0860
    Schlagwort(e): Leguminosae ; Morphology ; Stigma ; Style ; Vicia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The morphology and development of styles and stigmas ofVicia, one of the five genera of tribe Vicieae, were studied by scanning electron microscopy to contribute to a revision of infrageneric classification. Among the 22 species examined, variations were found as to: (1) whether a boundary between stigma and style is V-shaped when viewed from the adaxial side or not; (2) whether stylar hairs sprout on all sides of the style or only on the abaxial side; (3) whether a hairy area of the style connects with the stigma area first at lateral sides or at an adaxial side; (4) whether styles are tapered or bottle-neck-shaped; (5) whether styles are straight or bend abaxially. Based on these variations, five morphological types of styles and stigmas were provided with a diagnostic key. Comparisons with related tribes Cicereae, Coronilleae, Galegeae, Hedysareae, Loteae and Trifolieae as possible outgroups suggested that the V-shaped boundary between stigma and style is a synapomorphy of some species withinVicia, and that a dorsally compressed and abaxially tufted style, which was distributed in separate subgenera and sections sensu Kupicha but is now known to occur together with the V-shaped boundary, has evolved only one time in the genus, rather than more than once as supposed earlier.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02344301
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  • 63
    Digitale Medien
    Digitale Medien
    Liposome capillary electrophoresis for analysis of interactions between lipid bilayers and solutes (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1519-1523 
    Details
    ISSN: 0173-0835
    Schlagwort(e): Capillary electrophoresis ; Drug ; Interaction free energy ; Liposome ; Peptide ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Liposomes, which mimic biomembranes, were used as a pseudostationary phase in capillary zone electrophoresis. The decrease in the mobility of an analyte owing to the presence of liposomes reflected interaction between the analyte and the liposomes. Equations were derived to calculate the specific capacity factor Ks (the capacity factor, K′, normalized to the liposome concentration 1 M) from the migration times and to estimate the difference in free energy, Δ(ΔG0), of the weak analyte/liposome interactions. The order of Ks values for the drugs tested was aspirin 〈 salicylic acid 〈 warfarin 〈 sulfasalazine. The peptide TyrGlySerThrProGlyCysCys interacted more strongly with the liposomes (Ks = 10.1 M-1) than did TyrGlySerThrProGlySerSer (Ks = 9.1 M-1). These results were similar to those obtained earlier by immobilized liposome chromatography.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/elps.11501601251
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  • 64
    Digitale Medien
    Digitale Medien
    An examination of the peroxidases from Lupinus albus L. hypocotyls (1994)
    Springer
    Planta 194 (1994), S. 311-317 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Cell wall ; Conformer (peroxidase) ; Indole-3-acetic acid (oxidases) ; Leguminosae ; Lupinus (hypocotyl) ; Peroxidase (acidic, basic)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Peroxidases (EC 1.11.1.7) from hypocotyls of Lupinus albus L. cv. Rio Maior have been characterised using one- and two-dimensional, native electrophoretic techniques. Data are presented showing the complexity in charge and molecular size or shape of these peroxidases. We report the finding of a new acidic peroxidase and several new basic peroxidases in these hypocotyls, and of their stability to treatments considered to break ligand-induced variants and conformational variants derived from differences in polypeptide folding. Densitometric data demonstrate that these new peroxidases contribute up to 60‰ of the total peroxidase activity in hypocotyls. Studies of intercellular fluid, cell-wall and soluble fractions, with assays of purity were conducted in an attempt to define the subcellular locations of these additional peroxidases. The acidic form (pI 4.1) is greatly enriched in soluble fractions, three of the basic peroxidases (pIs 9.5, 9.7 and 〉9.7) are strongly associated to the cell wall, ad a minor, basic component (pI 9.7) is enriched in the intercellular fluid. Individual peroxidase activities with the substrates coniferyl alcohol, ferulic acid or indole acetic acid were compared by densitometric analysis of zymograms with those for guaiacol, and notable differences between these peroxidases in their capacity to oxidise indole acetic acid in vitro were identified. The possible functions of these peroxidases in vivo and their implications to current understanding of peroxidases in L. albus are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00197530
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  • 65
    Digitale Medien
    Digitale Medien
    Production of RNA by a polymerase protein encapsulated within phospholipid vesicles (1994)
    Springer
    Journal of molecular evolution 39 (1994), S. 555-559 
    Details
    ISSN: 1432-1432
    Schlagwort(e): RNA ; Liposome ; Biogenesis ; Origin of life ; Polynucleotide phosphorylase ; Polymerase ; Permeability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Catalyzed polymerization reactions represent a primary anabolic activity of all cells. It can be assumed that early cells carried out such reactions, in which macromolecular catalysts were encapsulated within some type of boundary membrane. In the experiments described here, we show that a template-independent RNA polymerase (polynucleotide phosphorylase) can be encapsulated in dimyristoyl phosphatidylcholine vesicles without substrate. When the substrate adenosine diphosphate (ADP) was provided externally, long-chain RNA polymers were synthesized within the vesicles. Substrate flux was maximized by maintaining the vesicles at the phase transition temperature of the component lipid. A protease was introduced externally as an additional control. Free enzyme was inactivated under identical conditions. RNA products were visualized in situ by ethidium bromide fluorescence. The products were harvested from the liposomes, radiolabeled, and analyzed by polyacrylamide gel electrophoresis. Encapsulated catalysts represent a model for primitive cellular systems in which an RNA polymerase was entrapped within a protected microenvironment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00160400
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  • 66
    Digitale Medien
    Digitale Medien
    Permeation of membranes by the neutral form of amino acids and peptides: Relevance to the origin of peptide translocation (1994)
    Springer
    Journal of molecular evolution 39 (1994), S. 1-5 
    Details
    ISSN: 1432-1432
    Schlagwort(e): Peptides ; Amino acid ; Weak bases ; Transmembrane pH gradient ; Liposome ; Biogenesis ; Permeability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The flux of amino acids and other nutrient solutes such as phosphate across lipid bilayers (liposomes) is 105 slower than facilitated inward transport across biological membranes. This suggests that primitive cells lacking highly evolved transport systems would have difficulty transporting sufficient nutrients for cell growth to occur. There are two possible ways by which early life may have overcome this difficulty: (1) The membranes of the earliest cellular life-forms may have been intrinsically more permeable to solutes; or (2) some transport mechanism may have been available to facilitate transbilayer movement of solutes essential for cell survival and growth prior to the evolution of membrane transport proteins. Translocation of neutral species represents one such mechanism. The neutral forms of amino acids modified by methylation (creating protonated weak bases) permeate membranes up to 1010 times faster than charged forms. This increased permeability when coupled to a transmembrane pH gradient can result in significantly increased rates of net unidirectional transport. Such pH gradients can be generated in vesicles used to model protocells that preceded and were presumably ancestral to early forms of life. This transport mechanism may still play a role in some protein translocation processes (e.g., for certain signal sequences, toxins and thylakoid proteins) in vivo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00178243
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  • 67
    Digitale Medien
    Digitale Medien
    Chloroplast DNA variation inDesmodium subgenusPodocarpium (Leguminosae): Infrageneric phylogeny and infraspecific variations (1994)
    Springer
    Journal of plant research 107 (1994), S. 349-354 
    Details
    ISSN: 1618-0860
    Schlagwort(e): Chloroplast DNA ; Desmodium ; Leguminosae ; Molecular phylogeny ; SubgenusPodocarpium ; Restriction site variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Chloroplast DNA (cpDNA) restriction site variation was examined in five species ofDesmodium subgenusPodocarpium (Leguminosae; Papilionoideae; Desmodieae). Twenty four phylogenetically informative cpDNA mutations were scored. The cladistic analysis of characters based on the 24 mutations resulted in the most parsimonious tree which supports the monophyly of the subgenus.Desmodium elegans of subgenusDollinera was the sister group of subgenusPodocarpium in this tree. The groupings obtained from the cpDNA characters were consistent with the present infrageneric classification system for the subgenus except for the infraspecific taxa ofD. podocarpum. Three groups withinD. podocarpum, which were incongruent with the infraspecific classification of the species, were distinguished by a total of four site mutations. The first group consisted of subsp.podocarpum, subsp.fallax, and subsp.oxyphyllum var.oxyphyllum; the second subsp.oxyphyllum var.oxyphyllum; and the last subsp.oxyphyllum var.oxyphyllum and var.mandshuricum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02344263
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  • 68
    Digitale Medien
    Digitale Medien
    Restriction fragment length polymorphisms of the phytohemagglutinin genes inPhaseolus andVigna (Leguminosae) (1994)
    Springer
    Plant systematics and evolution 191 (1994), S. 131-146 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Phaseolus ; Vigna ; Lectin genes ; phylogeny ; diversity ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Restriction fragment length polymorphisms at the phytohemagglutinin (PHA) locus were determined among 21 genotypes ofPhaseolus vulgaris, P. coccineus, P. acutifolius, P. lunatus, and threeVigna species, using five restriction enzymes and one double digestion, in order to provide molecular evidence for their genetic relatedness. The dissimilarity between genotypes was estimated from binary RFLP data. The dissimilarity was high among species (from 0.75 to 0.95), and of variable extent among genotypes of the same species (0.33–0.89). InP. vulgaris, two different DNA hybridization patterns were found, giving further evidence for two major gene pools in that species. The restriction patterns ofP. vulgaris var.aborigineus, the putative ancestral form ofP. vulgaris, exhibit clear homology toP. vulgaris genotypes. An undefined landrace from Taiwan could be identified as aP. vulgaris genotype. RFLP-based trees for the phytohemagglutinin genes of the species studied were computed with several distance matrix and parsimony methods.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984660
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  • 69
    Digitale Medien
    Digitale Medien
    To bee or not to bee?: The pollination biology ofApios americana (Leguminosae) (1994)
    Springer
    Plant systematics and evolution 192 (1994), S. 147-149 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Apios americana ; Bee pollination ; triploid clones
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The only hard evidence from field studies in the natural range ofApios americana (Leguminosae) indicates that this species is frequently triploid and seed sterile, and that the only legitimate pollinators are Megachilid bees. Claims of fly pollination based exclusively on examination of floral features of a European botanic garden specimen are unwarranted.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985913
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  • 70
    Digitale Medien
    Digitale Medien
    The characterisation and identification of a naturally occurring hybrid in the genusLeucaena (Leguminosae: Mimosoideae) (1994)
    Springer
    Plant systematics and evolution 192 (1994), S. 177-197 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Leucaena esculenta ; L. leucocephala ; Hybridization ; sterile triploid ; morphometric analysis ; DNA characters ; restriction site analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Phytogeographical, morphological, and molecular evidence for the widespread but sporadic occurrence of sterile hybrids betweenLeucaena leucocephala subsp.glabrata andL. esculenta subsp.esculenta in South-Central Mexico is presented. Most morphological and DNA characters studied in the putative hybrids showed states intermediate between the proposed parental taxa. The occurrence of non-additive nuclear ribosomal DNA phenotypes is discussed and the need to use a suite of nuclear taxon-specific markers to determine hybridity is emphasized. The origin of the hybrid is discussed in relation to the disruption of the distributions of both parental taxa through use by man as minor food plants, providing another example of the important influence of human interference on the evolution ofLeucaena. The successful use of dried leaf material as a source of DNA is highlighted as an efficient way to identify sterile hybrids at the molecular level.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00986251
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  • 71
    Digitale Medien
    Digitale Medien
    A phylogenetic analysis ofLeucaena (Leguminosae: Mimosoideae) (1994)
    Springer
    Plant systematics and evolution 191 (1994), S. 1-26 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Mimoseae ; Leucaena ; Phylogeny ; chloroplast DNA ; polyploidy ; evolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Chloroplast DNA restriction fragment length polymorphisms have been used to reconstruct the maternal phylogeny of all the known taxa in the small neotropical legume genusLeucaena. Three major plastome clades were recognized, but these did not conform with relationships between the taxa proposed on other characters from morphology, cytology or hybridization. The maternal parentage of tetraploids within the genus has been proposed. Evidence for introgression was found between “diploid”L. diversifolia and “tetraploid”L. diversifolia. The implications of these results for the origin of the cultivated taxa are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00985339
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  • 72
    Digitale Medien
    Digitale Medien
    Leaf anatomy inCaesalpinia andHoffmannseggia (Leguminosae, Caesalpinioideae) with emphasis on secretory structures (1994)
    Springer
    Plant systematics and evolution 192 (1994), S. 231-255 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Caesalpinioideae ; Caesalpinia ; Hoffmannseggia ; Crystals ; idioblasts ; glands ; leaf anatomy ; secretory cavities ; stomata ; trichomes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Leaflets of 65 species ofCaesalpinia s.l. and seven species ofHoffmannseggia were studied in clearings supplemented by resin sections and scanning electron microscopy. Three types of secretory structure occurred among 46 species; in 43 species they were distributed mutually exclusively (external glands: 8 species; internal cavities: 5 species; idioblastic cells: 30 species); three other species each had two types. Species with secretory structures conform mostly to proposed subgenera and informal “groups.” Other unusual features were external glands with internal spaces, thickened walls or conspicuous localized wall thickenings in epidermal cells or mesophyll cells of certain species, and differentially stained epidermal cells surrounding stomata. Prismatic crystals predominate but druse crystals also occur.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00986254
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  • 73
    Digitale Medien
    Digitale Medien
    Effect of microwave radiation on the permeability of carbonic anhydrase loaded unilamellar liposomes (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 15 (1994), S. 303-313 
    Details
    ISSN: 0197-8462
    Schlagwort(e): microwaves ; Liposome ; permeability changes ; enzyme ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Physik
    Notizen: The influence of 2.45 GHz microwave exposure (6 mW/g) on the diffusion processes in enzyme-loaded unilamellar liposomes as bioreactors was studied. The enzyme carbonic anhydrase (CA) was entrapped into cationic unilamellar vesicles. Previous kinetic experiments showed a very low self-diffusion rate of the substrate p-nitrophenyl acetate (PNPA) across intact liposome bilayer. A twofold increase in the diffusion rate of PNPA through the lipid bilayer was observed after 120 min of microwave radiation compared to temperature control samples. The microwave effect was time dependent. The enzyme activity, as a function of increased diffusion of PNPA, rises over 120 min from 22.3% to 80%. The increase in stearylamine concentration reduces the enzyme activity from 80% to 65% at 120 min. No enzyme leakage was observed. © 1994 Wiley-Liss, Inc.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bem.2250150405
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  • 74
    Digitale Medien
    Digitale Medien
    Asymmetric somatic hybrid plants between Medicago sativa L. (alfalfa, lucerne) and Onobrychis viciifolia Scop. (sainfoin) (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 455-463 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Intergeneric hybrid ; Protoplast fusion ; Leguminosae ; Total-genomic probing ; Bloat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract This paper reports on the production of intergeneric somatic hybrid plants between two sexually incompatible legume species. Medicago sativa (alfalfa, lucerne) leaf protoplasts were inactivated by lethal doses of iodoacetamide. Onobrychis viciifolia (sainfoin) suspension-cell protoplasts were gamma-irradiated at lethal doses. Following electrofusion under optimized conditions about 50,000 viable heterokaryons were produced in each test. The fusion products were cultured with the help of alfalfa nurse protoplasts. Functional complementation permitted only the heterokaryons to survive. A total of 706 putative heterokaryon-derived plantlets were regenerated and 570 survived transplantation to soil. Experimentation was aimed at the introduction of proanthocyanidins (condensed tannins) from sainfoin, a bloat-safe plant, to alfalfa, a bloat-causing forage crop; however, no tannin-positive regenerant plants were detected. Most regenerant plants have shown morphological differences from the fusion parents, although, as expected, all resembled the “recipient” parent, alfalfa. Southern analysis using an improved total-genomic probing technique has shown low levels of sainfoin-specific DNA in 43 out of 158 tested regenerants. Cytogenetic analysis of these asymmetric hybrids has confirmed the existence of euploid (2n=32; 17%) as well as aneuploid (2n=30, 33–78; 83%) plants. Pollen germination tests have indicated that the majority of the hybrids were fertile, while 35% had either reduced fertility or were completely sterile.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215091
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  • 75
    Digitale Medien
    Digitale Medien
    Transfer of bipiperidyl and quinolizidine alkaloids toViscum cruciatum Sieber (Loranthaceae) hemiparasitic onRetama sphaerocarpa boissier (Leguminosae) (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 2389-2393 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Viscum cruciatum ; Loranthaceae ; hemiparasites ; alkaloids ; Leguminosae ; Retama sphaerocarpa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Plant material ofViscum cruciatum Sieber contains bipiperidyl (ammodendrine) and quinolizidine alkaloids (lupanine, 5,6-dehydrolupanine, retamine, cytisine,N-methylcytisine). This plant obtains the alkaloids by root parasitism onRetama sphaerocarpa Boissier (host plant). These results have important implications forViscum ecology, for the study of herbivores that areViscum specialists, and in the development of systems for the investigation of the role of alkaloids as plant defenses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00979672
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  • 76
    Digitale Medien
    Digitale Medien
    Allelopathic potentials of alfalfa (Medicago sativa) saponins: Their relation to antifungal and hemolytic activities (1993)
    Springer
    Journal of chemical ecology 19 (1993), S. 1063-1074 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Medicago sativa L. ; Triticum aestivum L. ; Leguminosae ; saponins ; triterpenes ; biological activity ; allelopathy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A wheat seedling bioassay was used to indicate the relationship between the chemical structure of alfalfa saponins and their allelopathic activity. None of the tested saponins significantly influenced wheat germination. Seedling growth bioassays indicated significant differences among the activities of individual glycosides. The most active were medicagenic acid, its glycosides substituted at the C-3 position with glucose, and hederagenin monoglycoside. Medicagenic acid glycosides, substituted at the C-3 position with glucuronic acid, and zahnic acid tridesmoside were less active. No significant correlation was found among the allelopathic, hemolytic, and antifungal activities of the individual glycosides.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00987369
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  • 77
    Digitale Medien
    Digitale Medien
    Comparison of production efficiency among field crops related to nitrogen nutrition and application (1993)
    Springer
    Plant and soil 155-156 (1993), S. 207-210 
    Details
    ISSN: 1573-5036
    Schlagwort(e): growth efficiency ; Gramineae ; Leguminosae ; respiration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract It has been generally considered that the low productivity of Leguminosae is caused by accumulation in the reproductive organs of a large amount of protein and lipid, since the biochemical costs of synthesizing these compounds is higher than that for carbohydrate. However, we report here on results which show that: the growth efficiencies (dry matter accumulated/ (dry matter accumulated + respiration)) of reproductive organs of Gramineae and Leguminosae were similar; the growth efficiency of rice in the vegetative stage was greater than that of soybean and field bean, regardless of nitrogen application rate; and when 14CO2, 14C-sucrose or 14C-asparagine were introduced to the leaf at the maturation stage, respiratory loss of the introduced 14C was greater in soybean and field bean, especially in the light, than in rice. Thus, it is assumed that the low productivity in Leguminosae is caused by a larger respiratory loss under both dark and light condition in the shoot, and not in the reproductive organs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00025020
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  • 78
    Digitale Medien
    Digitale Medien
    Seedling morphology ofLespedeza (Leguminosae) (1993)
    Springer
    Journal of plant research 106 (1993), S. 121-128 
    Details
    ISSN: 1618-0860
    Schlagwort(e): Desmodieae ; Leguminosae ; Lespedeza ; Morphology ; Seedling
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Seedling morphology was studied in 22 species ofLespedeza, of which six belong to subgenusMacroles-pedeza and 16 to subgenusLespedeza. Two seedling types were recognized: 1) those with opposite leaves at the first node; 2) those having the first and subsequent leaves alternate. The two types are distinguished in the number of leaf primordia in the plumule of the seed: the opposite type has two leaf primordia, but the alternate type has only one primordium. Most species exhibited one of the two types, but rarely both types were observed in several species. In species having two types, one type always far outnumbers the other. The opposite type was common in Asian species, while the alternate type was common in North American ones. Because seedlings are of the opposite type inKummerowia which is the most closely related genus withLespdeza, the alternate type is considered to be apomorphic inLespedeza. The apomorphic seedling morphology is, there-fore, dominantly occurred in North American species ofLespedeza. This fact may be an evidence which suggests a monophyletic origin for North American species from an Asian ancesfor.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02344415
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  • 79
    Digitale Medien
    Digitale Medien
    DNA analysis of tropical plant species: An assessment of different drying methods (1993)
    Springer
    Plant systematics and evolution 188 (1993), S. 57-64 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Leguminosae ; Rubiaceae ; Vochysiaceae ; Leucaena ; Vochysia ; Qualea ; Coffea ; Molecular analysis ; drying methods ; ribosomal DNA ; restriction analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Use of molecular techniques to study plant systematics has been restricted to either largely temperate groups or groups from which seed is readily obtained. Dried material has advantages over other methods of preservation for molecular analyses; it is cheap, easily undertaken, overcomes the difficulties associated with seed recalcitrance and the absence of seeding plants in the field, and most taxonomists are familiar with the method. Three drying techniques were assessed using material collected in the tropics. No differences in either the quantity or the quality of DNA extracted from material dried by these methods was detected. The implications of using dried material in molecular analyses are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00937835
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  • 80
    Digitale Medien
    Digitale Medien
    Some polyphagous Homoptera gain protection from their natural enemies by feeding on the toxic plantsSpartium junceum andErythrina corallodendrum (Leguminosae) (1992)
    Springer
    Chemoecology 3 (1992), S. 118-124 
    Details
    ISSN: 1423-0445
    Schlagwort(e): chemical defence ; tri-trophic interactions ; alkaloids ; Homoptera ; Aphis craccivora ; Icerya spp. ; Lepidosaphes ulmi ; Planococcus citri ; Coleoptera ; Coccinellidae ; Cryptolaemus montrouzieri ; Rodolia cardinalis ; Chilocorus bipustulatus ; Neuroptera ; Chrysopidae ; Chrysoperla carnea ; Hymenoptera ; Encyrtidae ; Encyrtus infelix ; Leguminosae ; Erythrina corallodendrum ; Spartium junceum ; Citrus sinensis ; Euphorbia tirucalli ; Pittosporum tobira
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Interactions were studied among alkaloid-containing legumes (Erythrina corallodendrum andSpartium junceum) and non-toxic plants (Citrus sinensis, Cucurbita moschata andEuphorbia tirucalli), several polyphagous homopterans,Aphis craccivora (Aphididae),Icerya purchasi, I. aegyptiaca (Margarodidae),Lepidosaphes ulmi (Diaspididae) andPlanococcus citri (Pseudococcidae), and some major natural enemies of these homopterans. Significant reductions in survival due to negative effects of alkaloid containing as compared with non-alkaloidal plants were recorded for the predatorsRodolia cardinalis andChilocorus bipustulatus, but not forCryptolaemus montrouzieri (Coleoptera: Coccinellidae),Chrysoperla carnea (Neuroptera: Chrysopidae) andSympherobius sanctus (Neuroptera: Sympherobiidae). The development time of the larvae or pupae ofR. cardinalis, C. carnea andS. sanctus was longer on the toxic plants than on the non-toxic ones. The percentage of parasitism ofA. craccivora collected from the non-alkaloidal plantsVicia palaestina andMelilotus albus was much higher than that onS. junceum. The parasitoid complexes ofA. craccivora differed between both plant groups. The nutritive value of honeydew ofI. purchasi andA. craccivora, as expressed by the life span ofEncyrtus infelix (Hymenoptera: Encyrtidae) adults, was also investigated. Life spans were significantly longer when the wasps fed on honeydew produced on non-alkaloidal plants (C. sinensis andPittosporum tobira) than on alkaloid containing plants whenI. purchasi — but notA. craccivora — was the producer. It is suggested that the chemical defense ofE. corallodendrum andS. junceum is exploited by polyphagous phytophages to reduce predation. In nature, population growth and density of four of the investigated homopterans are conspicuously high when they developed on the alkaloid containing plant species, and very low on non-alkaloid plants. The efficiency of their natural enemies may be reduced by sequestration of alkaloids (or other toxic plant compounds) or their transfer into excreted honeydew. Therefore it is assumed that a generalist phytophagous homopteran may be protected from its natural enemies, although at different rates of efficiency, if it can safely sequester the host allelochemical when it develops on toxic species within its host range.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01370139
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  • 81
    Digitale Medien
    Digitale Medien
    Compensatory response of a legume root-nodule system to nodule herbivory by Sitona hispidulus (1992)
    Springer
    Entomologia experimentalis et applicata 64 (1992), S. 167-176 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Curculionidae ; Sitona hispidulus ; Leguminosae ; nodule herbivory ; compensatory growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A laboratory study was conducted to examine the hypothesis that herbivory of nitrogen-fixing root nodules on legumes causes an exact compensatory response in nodule growth. Plants of Medicago sativa (L.) were grown hydroponically in clear plastic growth pouches so that the number and biomass of root nodules could be estimated nondestructively before, and 10 and 18 days after, partial denodulation. For treatments, plants were subjected to 23% denodulation by first-instar larvae of Sitona hispidulus (F.) (a common herbivore of Medicago and Trifolium) or 50% nodule pruning; additional plants were left untreated. Results indicated that nodule herbivory and nodule pruning caused an overcompensatory response in number of nodules. This was also true for number of nodule units (an indirect measure of nodule biomass) per plant at 10 days after denodulation but had changed to an exact compensatory response by day 18. An inverse relationship between change in number of nodule units and initial number of nodules indicated that compensatory nodulation was regulated by a feedback mechanism. Shoot and root biomasses were not affected by denodulation in this study.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00162989
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  • 82
    Digitale Medien
    Digitale Medien
    Hymenaea protera sp.n. (Leguminosae, Caesalpinioideae) from Dominican amber has African affinities (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 1075-1082 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Hymenaea ; Leguminosae ; Caesalpinioideae ; fossil ; amber ; Dominican Republic
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Hymenaea protera is described from amber originating from La Toca mine in the Dominican Republic. The fossil species is characterized by the presence of reduced petals as well as distinctly clawed showy petals with cordate to reniform bases and a glabrous, verrucose ovary with long hirsute hairs at its base and along one margin. The fossil species most closely resembles the extantH. verrucosa Gaertner which occurs in East Africa and adjacent islands. These findings are interpreted as supporting a hypothesis that the genusHymenaea arose in the late Cretaceous on the combined South American-African continents and that Cretaceous and early Tertiary landmass movements were significant in determining the present amphi-Atlantic distribution of the genus. It is proposed that at least the majority, if not all, of the amber recovered from La Toca mine and other mines in the vicinity with similar-aged deposits originated fromH. protera.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01923347
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  • 83
    Digitale Medien
    Digitale Medien
    Immunosome technology to improve antigen presentation for efficient and safe viral vaccines (1991)
    Springer
    Molecular engineering 1 (1991), S. 275-293 
    Details
    ISSN: 1572-8951
    Schlagwort(e): Liposome ; envelope glycoprotein ; antigen presentation ; subunit vaccine ; protection ; influenza virus ; rabies virus ; human immunodeficiency virus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The role of liposomes in antigen presentation and in induction of humoral and cell-mediated immune responses has been investigated by anchoring viral envelope glycoproteins into the phospholipidic bilayer of preformed liposomes to produce Immunosomes. Using purified glycoproteins of three different enveloped viruses, it was found that in mice immunosomes induced high titres of neutralizing antibodies, whereas equal amounts of the purified glycoproteins alone failed to induce or induced much lower neutralizing antibodies. Similarly, immunosomes induced in vaccinated animals antigenspecific interleukin-2 production upon in vitro restimulation with the same antigen, whereas no secondary response was observed in animals vaccinated with equal amounts of the free antigen. Finally, influenza and rabies immunosomes were shown to be efficient in protecting animals from a challenge with the corresponding virulent strain.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00161669
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  • 84
    Digitale Medien
    Digitale Medien
    Primary structure of kunitz-type trypsin inhibitor-2a (pI 5.9) fromPsophocarpus tetragonolobus (L.) DC seed (1991)
    Springer
    The protein journal 10 (1991), S. 183-188 
    Details
    ISSN: 1573-4943
    Schlagwort(e): Trypsin inhibitor ; Kunitz-type seed inhibitor ; amino acid sequence ; sequence similarity ; Leguminosae ; winged bean ; Psophocarpus tetragonolobus (L.) DC
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The primary structure of acidic trypsin inhibitor-2a (WBTI-2a,pI 5.9) fromPsophocarpus tetragonolobus (L.) DC seed was determined. This inhibitor consists of a single polypeptide chain of 180 amino acids including four half-cystine residues and has an N-terminal residue of pyroglutamic acid. The sequence of WBTI-2a,pI 5.9, showed 84% identity to acidic trypsin inhibitor-2 (WBTI-2,pI 5.1) but only 57% identity to the basic trypsin inhibitor (WBTI-1,pI 8.9) and 50% identity to the chymotrypsin inhibitor of winged bean. The data indicate that winged bean seed contains a family of three Kunitz-type inhibitors which have about 50% identity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01024782
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  • 85
    Digitale Medien
    Digitale Medien
    Reaction characteristics and stability of a membrane-bound enzyme reconstituted in bilayers of liposomes (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 809-813 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Membrane-bound enzyme ; Reconstitution ; Liposome ; Immobilization ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The effects ensuing from the interaction between membrane-bound sarcosine dehydrogenase and the surrounding lipids as well as the effects of membrane fluidity were described in this study. A 25-fold activation was observed upon the reconstitution of the enzyme in bilayers of SUVs made of DMPC. The considerable decrease in Km and increase in Vmax suggest the induction of favorable conformational changes in both the substrate-binding site and the catalytic site of the enzyme due to the lipid-protein interaction. In SUVs of negatively charged phospholipids, the enzyme retained its initial activity over 1 month. The break point in the Arrhenius plot of the activity of reconstituted enzyme was found at temperatures close to the gel-liquid crystalline transition point of the phospholipid showing that the activity is sensitive to the physical state of membrane phospholipids. Further, immobilization of the reconstituted enzyme by use of ENT prepolymer resulted in a high activity, whereas no remarkable activity was detected with the immobilized enzyme without reconstitution.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260370904
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  • 86
    Digitale Medien
    Digitale Medien
    Medicago truncatula f.laxicycla (Leguminosae), a new taxon with loose fruit coiling promoting rapid germination of the fruit-retained seeds (1991)
    Springer
    Plant systematics and evolution 174 (1991), S. 37-46 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Medicago truncatula f.laxicycla ; M. laxispira ; Evolution ; adaptation ; seed germination ; chromosome number ; Flora of Libya
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Medicago truncatula is a member ofMedicago sect.Spirocarpos, in which all 34 species have coiled fruit, usually tightly coiled, that permanently retain the seeds. A variant ofM. truncatula from Libya is unique for the species in possessing very loosely coiled fruits, and is here recognized as a new forma,M. truncatula f.laxicycla E. Small. In germination tests of a number of species ofMedicago, the unusual Libyan variant proved to be the only one in which rapid and simultaneous germination of most of the seeds occurred while still within a fruit. The loose coiling of the fruit of the Libyan variety appears to facilitate water entry and seedling escape. Its rapid germination might adapt it to respond quickly to intermittently available moisture, but at the cost of abandoning the strategy of intermittent germination adopted by other populations ofMedicago sect.Spirocarpos.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00937692
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  • 87
    Digitale Medien
    Digitale Medien
    Allozyme variation and phylogeny in annual species ofCicer (Leguminosae) (1991)
    Springer
    Plant systematics and evolution 175 (1991), S. 11-21 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Cicer ; Allozymes ; isozyme gene duplication ; phylogenetic relations ; polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Allozymic variation at 30 isozyme loci was examined electrophoretically in nine annual and one perennial species ofCicer. While most of the accessions examined were monomorphic, species can be differentiated on the basis of their enzyme phenotypes. Several groups of species were identified based upon genetic distance values. For example,C. arietinum, C. reticulatum, andC. echinospermum shared the same alleles for most of the loci exmained. PerennialC. anatolicum is also closely related to this group. Similarly,C. judaicum, C. bijugum, andC. pinnatifidum formed another group. Two annual species,C. chorassanicum andC. yamashitae clustered together, whereasC. cuneatum was the most distantly related species. Correlations were found between genetic distances and geographic distribution. Results from enzyme electrophoresis tend to support the previously reported taxonomic treatments based upon crossability and morphological similarity. However,C. yamashitae, which has been classified in the second crossability group, is quite distinct genetically and morphologically from the remaining species of the group. An isozyme gene duplication observed in the genus suggested the monophyletic origin of the species examined in the present study.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00942142
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  • 88
    Digitale Medien
    Digitale Medien
    Cytotaxonomic studies of Eastern MediterraneanVicia species (Leguminosae) (1991)
    Springer
    Plant systematics and evolution 177 (1991), S. 221-234 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Papilionoideae ; Vicieae ; Vicia ; Vetch ; chromosome number ; DNA amount ; microdensitometry ; cytotaxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Collected material of rare and newVicia spp. was investigated from a cytotaxonomic point of view. Nineteen species were examined in total. The study includes 14 novel chromosome counts and measurements of DNA amount, as well as a comparison of chromosome morphology between species. The results of the cytological study support to an extent a recent taxonomical revision of subgenusVicia.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00937959
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  • 89
    Digitale Medien
    Digitale Medien
    Translocation of Ca2+ across lipid bilayer membrane due to defects induced by teleocidin (1990)
    Springer
    Colloid & polymer science 268 (1990), S. 1052-1058 
    Details
    ISSN: 1435-1536
    Schlagwort(e): Liposome ; Ca2+ translocation ; phosphatidylcholine ; teleocidin ; TPA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Maschinenbau
    Notizen: Abstract The effect of defects in a dipalmitoylphosphatidylcholine (DPPC) membrane on Ca2+ permeability across the membrane was studied. Addition of teleocidin to a suspension of DPPC vesicles encapsulating Quin 2 increased the fluorescence intensity of Quin 2. Change of fluorescence intensity was significant below the phase-transition temperature of the membrane, and increased according to the kind of divalent metal ions in the medium in the order of Mg2+〈Ba2+〈Ca2+. It was confirmed that DPPC vesicles did not change the vesicular structure upon binding teleocidin to the membrane. Therefore, the fluorescence increase below the phase-transition temperature was ascribed to the influx of divalent cations into DPPC vesicles through cracks formed in the membrane upon distribution of teleocidin. By contrast, 12-0-tetradecanoylphorbol-13-acetate (TPA) did not change the fluorescence intensity of Quin 2 significantly. It should be noted that teleocidin, which located at the membrane surface, yielded more significant defects across the lipid membrane than TPA, which was incorporated into the hydrophobic core of the membrane.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01410594
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  • 90
    Digitale Medien
    Digitale Medien
    Nuclear DNA variability withinPisum sativum (Leguminosae): Cytophotometric analyses (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 179-185 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Pisum sativum ; Cytophotometry ; nuclear DNA content ; DNA variability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The cultivars or experimental lines ofPisum sativum were analyzed cytophotometrically for nuclear DNA content of early prophases after ethanol-acetic acid fixation. Wide variability was found (from 3.93 to 5.07pg per haploid nucleus). This result was confirmed by the cytophotometric analysis of interphase nuclei isolated from leaf tissues fixed in formalin. Analysis of interphase nuclei at different thresholds of optical density showed that certain chromatin fractions are involved in the variations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00940861
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  • 91
    Digitale Medien
    Digitale Medien
    Metabolism of [1,2-3H]gibberellin A4 by epicotyls and cell-free preparations from Phaseolus coccineus L. seedlings (1989)
    Springer
    Planta 178 (1989), S. 267-274 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Gibberellin metabolism (in vitro, in vivo) ; Gibberellin transport ; Leguminosae ; Phaseolus (gibberellin metabolism) ; Seedling
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cell-free systems were prepared from germinating seed and seedlings of Phaseolus coccineus. Gibberellin A4 (GA4)-metabolising activity was detected in vitro using preparations from roots, shoots and cotyledons of germinating seed, but only up to 24 h after imbibition. Cell-free preparations from cotyledons converted [3H]GA4 to GA1, GA34, GA4-glucosyl ester and a putative O-glucoside of GA34, and, in addition converted [3H]GA1 to GA8. Preparations from embryo tissues contained 2β-hydroxylase activity, converting [3H]GA4 to GA34 and [3H]GA1 to GA8. The presence of GA-metabolising enzymes was also indicated by in-vivo feeds of [3H]GA4 to epicotyls of intact 4-d-old seedlings, which resulted in the accumulation of GA1, GA8, GA3-3-O-glucoside, GA4-glucosyl ester, GA8-2-O-glucoside and a putative O-glucoside of GA34. Gibberellin A1 was the first metabolite detected, 15 min after application of [3H]GA4, but after 24 h most of the label was associated with GA8-2-O-glucoside. Over 90% of the recovered radioactivity was found in the shoot. Within the shoot, movement was preferentially acropetal, and was not dependent upon metabolism of the applied [3H]GA4.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00393203
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  • 92
    Digitale Medien
    Digitale Medien
    Preferential infestation of fruits within theMedicago truncatula — M. littoralis (Leguminosae) complex by the bean weevilBruchidius bimaculatus (1989)
    Springer
    Plant systematics and evolution 163 (1989), S. 35-41 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Medicago truncatula ; Medicago littoralis ; Bruchidius bimaculatus ; Bruchid infestation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The bean weevilBruchidius bimaculatus was found in fruits of 68 of 217 populations of theMedicago truncatula — M. littoralis complex of Israel, Greece, Italy and Spain. Infestation was higher in fruits of somewhat larger than mean size, and in the more pubescent and tightly coiled fruits. Bean weevil size proved to be independent of fruit size, so that the “preference” for larger fruits does not seem due to adaptation to a larger food source or a larger domicile. Fruit size has been used to delimit the host “species”M. truncatula andM. littoralis, but the character proved to be unimodal, and it is apparent that the taxonomy of the plant complex requires further clarification. Of the 11 472 fruits examined, less than 4% were infested. Given that the plants have evolved indehiscent legumes in which typically only one of the approximately six seeds survives to reproduce, and that very few seeds of a fruit are destroyed, the bruchid's infestation appears to be non-harmful, and indeed the association may be mutualistic.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00936151
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  • 93
    Digitale Medien
    Digitale Medien
    Karyotypic and chloroplast genomic diversity inMedicago sect.Lupularia (Leguminosae) (1989)
    Springer
    Plant systematics and evolution 166 (1989), S. 69-78 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Medicago lupulina ; M. secundiflora ; Chromosomes ; karyotypes ; chloroplast DNA evolution ; phylogeny
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Studies were made on the chromosome complements and chloroplast genomes ofMedicago lupulina andM. secundiflora, which comprise sectionLupularia ofMedicago. Both types of analyses indicated more substantial differences between these species than suggested by external morphology.Medicago lupulina has a relatively asymmetrical karyotype in terms of centromeric position and relative length. The karyotype ofM. secundiflora is comparatively more asymmetrical in centromeric position and reduced in absolute size but exhibits greater symmetry in relative length. The restriction endonuclease fragmentation patterns of the chloropiast DNA of these two species (with Bam HI, Eco RI, Bgl II, and Xho I) show little similarity, with only 17% of the fragments matching in size. The lack of interspecific congruence among data of morphology, karyology and cpDNA inLupularia is contrary to consistency exhibited among these data inMedicago subsect.Intertextae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00937876
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  • 94
    Digitale Medien
    Digitale Medien
    A serological contribution to the systematics of the genusLupinus (Fabaceae) (1989)
    Springer
    Plant systematics and evolution 166 (1989), S. 265-278 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Fabaceae ; Genisteae ; Sophoreae ; Thermopsideae ; Lupinus ; Serological systematics ; phylogeny
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The immunological comparison of the seed reserve proteins suggests thatLupinus is a natural genus, the American and the Euro-African species belonging to the same stock. Among the Lupines of the Old World, the smooth-seeded and the rough-seeded species from two natural segregates. The genusLupinus is serologically related to the rest of theGenisteae and to the AsiaticSophoreae rather than to AmericanSophoreae andThermopsideae. The data suggest thatLupinus may have originated with the remainder of theGenisteae from primitiveSophoreae of temperate-subtropical Asia. America and the Mediterranean-African region are regarded as secondary centres of speciation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00935955
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  • 95
    Digitale Medien
    Digitale Medien
    Freeze-fracture of lipids and model membrane systems (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 13 (1989), S. 277-287 
    Details
    ISSN: 0741-0581
    Schlagwort(e): Liposome ; Unilamellar vesicle ; Hexagonal HII phase ; Lipidic particle ; Filipin ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Allgemeine Naturwissenschaft
    Notizen: Model membrane systems are used extensively in all aspects of membrane research, and freeze-fracture is the preeminent procedure for directly visualizing local structure in these lipid dispersions. Here we describe in detail the formation of liposomes and how freeze-fracture is routinely employed as a complementary technique to biophysical and biochemical procedures in the characterization of multilamellar vesicles (most commonly known as liposomes) and unilamellar vesicles. Many preparative procedures exist for the formation of multi- and unilamellar vesicles. Examples of each system are given and their properties as well as freeze-fracture morphology are discussed. The detection of lipid-phase transitions is considered, in particular, with emphasis on the application of freeze-fracture to the study of lipid polymorphism. We briefly discuss the fracturing of apolar lipids which do not adopt bilayer structures but which can be stabilized into microemulsions by a phospholipid monolayer. Finally, a critical assessment is made of filipin as a morphological marker for cholesterol domains in the plane of the bilayer.
    Zusätzliches Material: 11 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jemt.1060130403
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  • 96
    Digitale Medien
    Digitale Medien
    Pollen-ovule patterns in tribeTrifolieae (Leguminosae) (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 195-205 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Fabaceae ; Leguminosae ; Medicago ; Trigonella ; Melilotus ; Trifolium ; Parochetus ; Ononis ; Pollen ; pollination ; ovule ; adaptation ; evolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Relative pollen and ovule production in the genera of the legume tribeTrifolieae is explored particularly as to howMedicago with its explosive pollination mechanism compares with its allies.Medicago produces much larger, although much fewer pollen per ovule than the other five genera in the tribe; this is interpreted as a consequence of its highly specialized, irreversible pollination mechanism, which allows only one effective exchange of pollen with pollinators.Melilotus andTrifolium produce a comparatively large quantity of pollen; this can be related to their floral characteristics requiring abundant pollen to achieve fertilization. InMedicago andTrigonella, annuals convert a higher proportion of pollen grains and ovules into seeds than perennials.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00936047
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  • 97
    Digitale Medien
    Digitale Medien
    Transfer of pyrrolizidine and quinolizidine alkaloids toCastilleja (Scrophulariaceae) hemiparasites from composite and legume host plants (1987)
    Springer
    Journal of chemical ecology 13 (1987), S. 1917-1925 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Castilleja ; Scrophulariaceae ; Senecio ; Asteraceae ; Lupinus ; Leguminosae ; hemiparasites ; alkaloids ; plant-insect interactions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Castilleja (Scrophulariaceae) species of the western United States contain pyrrolizidine and quinolizidine alkaloids. TheCastilleja obtain the alkaloids by root parasitism on host plants, withSenecio atratus andS. triangularis (Asteraceae) furnishing the pyrrolizidines, and quinolizidines being obtained fromLupinus species andThermopsis montana (Leguminosae). Individual plants within a givenCastilleja species population may be devoid of alkaloids while others have high alkaloid content. No populations have been found which are made up of both pyrrolizidine- and quinolizidine-containing individuals. These results have important implications forCastilleja ecology, for the study of insect herbivores which areCastilleja specialists, and in the development of systems for the investigation of the role of alkaloids as plant defenses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01013240
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  • 98
    Digitale Medien
    Digitale Medien
    Relationship between tolerance and accumulation characteristics of cadmium in higher plants (1987)
    Springer
    Plant and soil 104 (1987), S. 275-280 
    Details
    ISSN: 1573-5036
    Schlagwort(e): accumulation ; Cd ; Cruciferae ; Cucurbitaceae ; Gramineae ; Leguminosae ; NH4NO3-extractable Cd ; sand soil ; soil pH ; Solanaceae ; Taxonomy ; tolerance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Ten plant species belonging to 5 families,i.e., Cruciferae, Cucurbitaceae, Gramineae, Leguminosae, and Solanaceae, were grown in a sand soil at two pH levels. The soil was subjected to an application of CdCl2 at rates of 0 to 700 mgCd·kg−1 soil. The relationship between Cd concentration in the shoots (tc) and soil (sc; NH4NO3 extractable) was expressed by the equation: log (tc)=α+βlog (sc). The coefficients α and β were estimated for each species at each level of soil pH. Plottings of the scores on α and β axes showed that the Cd accumulation characteristics in the plants appeared to depend on the families irrespective of soil pH. Based on theupper critical concentration of Cd in the tops (Ct), the Cruciferae and Leguminosae species were found to be the most and the least tolerant to Cd, respectively. The Ct values correlated exceedingly well with the values of α.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02372542
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  • 99
    Digitale Medien
    Digitale Medien
    Plant regeneration from leaf-derived callus cultures of the tropical pasture legume Stylosanthes scabra Vog. (1987)
    Springer
    Plant cell, tissue and organ culture 9 (1987), S. 3-8 
    Details
    ISSN: 1573-5044
    Schlagwort(e): Stylosanthes scabra ; callus culture ; organogenesis ; regeneration ; Leguminosae ; in vitro
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Callus cultures of 5 genotypes of S. scabra Vog. were optimally established from leaf tissue on Murashige and Skoog (MS) basal medium supplemented with 0.5–2.0 mg l-1 2, 4-Dichlorophenoxy acetic acid (2, 4-D) and 1.0–2.0 mg l-1 6-benzylaminopurine (BAP). On media containing 2, 4-D only, calli were soft, and rhizogenesis occurred on calli of 4 genotypes. Calli formed on media containing BAP only, but not with kinetin only. In the presence of 2, 4-D, BAP inhibited rhizogenesis and promoted better callus growth than kinetin. High frequency shoot induction was achieved for 3 genotypes on MS +2.0 mg l-1 BAP. Roots formed on shoots when sub-cultured on half-strenght MS without growth regulators. The form of cytokinin used in the callus induction media appeared to affect subsequent shoot organogenesis. Genotypic differences were observed for shoot organogenesis. There was some morphological variation evident among regenerants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00046073
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  • 100
    Digitale Medien
    Digitale Medien
    Onobrychis aliacmonia (Leguminosae) — the unusual story of a rediscovery (1987)
    Springer
    Plant systematics and evolution 155 (1987), S. 215-217 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Fabaceae ; Onobrychis aliacmonia ; Endemism ; threatened plants ; rediscovery ; Flora of Greece (Makedhonia, Peloponnisos)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Onobrychis aliacmonia was discovered in 1956 and described in 1973, the year when it was last collected. Its single known locality was flooded soon afterward by an artificial lake now extending over the middle portion of the Aliakmon Valley (western Greek Makedhonia), and the species was believed extinct. It has now been rediscovered in the southern Peloponnisos, in an area far south of the locus classicus and in a widely different ecological context.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00936300
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