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Restriction fragment length polymorphisms of the phytohemagglutinin genes inPhaseolus andVigna (Leguminosae)

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Abstract

Restriction fragment length polymorphisms at the phytohemagglutinin (PHA) locus were determined among 21 genotypes ofPhaseolus vulgaris, P. coccineus, P. acutifolius, P. lunatus, and threeVigna species, using five restriction enzymes and one double digestion, in order to provide molecular evidence for their genetic relatedness. The dissimilarity between genotypes was estimated from binary RFLP data. The dissimilarity was high among species (from 0.75 to 0.95), and of variable extent among genotypes of the same species (0.33–0.89). InP. vulgaris, two different DNA hybridization patterns were found, giving further evidence for two major gene pools in that species. The restriction patterns ofP. vulgaris var.aborigineus, the putative ancestral form ofP. vulgaris, exhibit clear homology toP. vulgaris genotypes. An undefined landrace from Taiwan could be identified as aP. vulgaris genotype. RFLP-based trees for the phytohemagglutinin genes of the species studied were computed with several distance matrix and parsimony methods.

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Authors and Affiliations

  1. Department of Biology, University of Kaiserslautern, P.O. Box 3049, D-67653, Kaiserslautern, Germany

    Dorothea Zink, Klaus Schumann & Walter Nagl

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  1. Dorothea Zink
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  2. Klaus Schumann
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  3. Walter Nagl
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Zink, D., Schumann, K. & Nagl, W. Restriction fragment length polymorphisms of the phytohemagglutinin genes inPhaseolus andVigna (Leguminosae). Pl Syst Evol 191, 131–146 (1994). https://doi.org/10.1007/BF00984660

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  • DOI: https://doi.org/10.1007/BF00984660

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