Abstract
Phytogeographical, morphological, and molecular evidence for the widespread but sporadic occurrence of sterile hybrids betweenLeucaena leucocephala subsp.glabrata andL. esculenta subsp.esculenta in South-Central Mexico is presented. Most morphological and DNA characters studied in the putative hybrids showed states intermediate between the proposed parental taxa. The occurrence of non-additive nuclear ribosomal DNA phenotypes is discussed and the need to use a suite of nuclear taxon-specific markers to determine hybridity is emphasized. The origin of the hybrid is discussed in relation to the disruption of the distributions of both parental taxa through use by man as minor food plants, providing another example of the important influence of human interference on the evolution ofLeucaena. The successful use of dried leaf material as a source of DNA is highlighted as an efficient way to identify sterile hybrids at the molecular level.
Similar content being viewed by others
References
Abbott, R. J., 1992: Plant invasions, interspecific hybridisation and the evolution of new taxa. — Trends Ecol. Evol.7: 401–405.
—, Ashton, P. A., Forbes, D. G., 1992: Introgressive origin of the radiate groundsel,Senecio vulgaris L. var.hibernicus Syme. — Heredity68: 425–435.
Adams, R. P., 1982: A comparison of multivariate methods for the detection of hybridization. — Taxon31: 646–661.
Alston, R. E., Turner, B. L., 1963: Natural hybridization among four species ofBaptisia (Leguminosae). — Amer. J. Bot.50: 159–173.
Anderson, E., 1949: Introgressive hybridization. — New York: Wiley.
Ashton, P. A., Abbott, R. J., 1992: Multiple origins and genetic diversity in the newly arisen allopolyploid speciesSenecio cambrensis Rosser (Compositae). — Heredity68: 25–32.
Brenan, J. P. M., 1959:Leguminosae. Mimosoideae. — InHubbard, C. E., Milne-Redhead, E., (Eds): Flora of Tropical East Africa. — London: Crown Agents for Overseas Governments and Administration.
Brewbaker, J. L., 1987:Leucaena: a multipurpose tree genus for tropical agroforestry. — InSteppler, H. A., Nair, P. K., (Eds): Agroforestry: a decade of development, pp. 289–322. — Nairobi, Kenya: Int. Council for Research on Agroforestry.
—, Sorensson, C. T., 1990:Leucaena: new tree crops from interspecificLeucaena hybrids. — InJanick, J., Simon, J., (Eds): Advances in new crops, pp. 283–289. — Oregon, USA: Timber Press.
Casas, A., 1992: Etnobotánica y procesos de domesticación enLeucaena esculenta (Moç. & Sessé exA.D.C.)Benth. — MSc Thesis, México: Facultad de Ciencias, Universidad Auton. Nac. de México.
Chase, M. W., Hills, H. H., 1991: Silica gel: An ideal material for field preservation of leaf samples for DNA studies. — Taxon40: 215–220.
De Freitas, L. H. C., Whitmann, M. T. S., Paim, N. R., 1991: Floral characteristics, chromosome number and meiotic behaviour of hybrids betweenLeucaena leucocephala (2n = 104) and tetraploidL. diversifolia(2n = 104) (Leguminosae). — Rev. Brasil. Genet.14: 781–789.
Doyle, J. J., 1991: DNA protocols for plants. — InHewitt, G. M., Johnston, A. W. B., Young, J. P. W., (Eds): Molecular techniques in taxonomy. — NATO ASI Series H,57, pp. 283–285. — Berlin, Heidelberg, New York: Springer.
—, Brown, A. H. D., 1989: 5s nuclear ribosomal gene variation in theGlycine tomentella polyploid complex. — Syst. Bot.14: 621–624.
—, Beachy, R. N., Lewis, W. H., 1984: Evolution of rDNAs inClaytonia polyploid complexes. — InGrant, W. F., (Ed.): Plant biosystematics, pp. 321–341. — London: Academic Press.
Ebinger, J. E., Seigler, D. S., 1987: Introgressive hybridization in Mexican populations ofAcacia macracantha andA. pennatula. — Bull. Int. Group StudyMimosoideae 15: 73–85.
Elamin, H. M., 1976:Acacia laeta R. Br. exBenth., considered as a species of hybrid origin. — Sudan Silva21: 14–23.
Fabijanski, S., Fedak, G., Armstrong, K., Altosaar, I., 1990: A repeated sequence probe for the C genome inAvena (oats). — Theor. Appl. Genet.79: 1–7.
Feinberg, A. P., Vogelstein, B., 1983: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. — Anal. Biochem.132: 6–13.
Filer, D. L., 1993: BRAHMS: Botanical research and herbarium management system. Reference manual version 3.1 April 1993. — Oxford: University of Oxford.
Flavell, R. B., O'Dell, M., Thompson, W. F., Vincentz, M., Sardana, R., Barker, R. F., 1986: The differential expression of ribosomal DNA genes. — Philos. Trans. Roy. Soc. London B314: 385–397.
Frahm-Leliveld, J. A., 1957: Observations cytologiques sur quelques Leguminoses tropicales et subtropicales. — Rev. Cytol. Biol. Veg.8: 273–287.
—, 1960: Chromosome numbers in leguminous plants. — Acta Bot. Neerl.9: 327–329.
Gallez, G. P., Gottlieb, L. D., 1982: Genetic evidence for the hybrid origin of the diploid plantStephanomeria diegensis. — Evolution36: 1158–1167.
Gerlach, W. L., Bedbrook, J. R., 1979: Cloning and characterisation of ribosomal RNA genes from wheat and barley. — Nucleic Acids Res.7: 1869–1885.
Gonzalez, V., Brewbaker, J. L., Hamill, D. E., 1967:Leucaena cytogenetics in relation to the breeding of low mimosine lines. — Crop Sci.7: 140–143.
Gottlieb, L. D., 1972: Levels of confidence in the analysis of hybridization in plants. — Ann. Missouri Bot. Gard.59: 435–446.
—, 1977: Electrophoretic evidence and plant systematics. — Ann. Missouri Bot. Gard.64: 161–180.
Grant, V., 1981: Plant speciation. — New York, London: Columbia University Press.
Harris, S. A., 1993: DNA analysis of tropical plant species: An assessment of different drying methods. — Pl. Syst. Evol.188: 57–64.
—, Ingram, R., 1992: Molecular systematics of the genusSenecio L. I: hybridization in a British polyploid complex. — Heredity69: 1–10.
—, Chamberlain, J. R., Hughes, C. E., 1994: New insights into the evolution ofLeucaena Benth. — InPolhill, R. M., Pickersgill, B., (Eds): Advances in legume systematics. — London: Royal Botanic Gardens, Kew (in press).
Hasler, H. J., 1983: AnAcacia hybrid on the Mornington Peninsula, Victoria. — Bull. Int. Group StudyMimosoideae 11: 38–42.
Hernandez, H. M., 1991: Taxonomía, distribución geográfica y biología reproductiva deCalliandra calothyrsus (Leguminosae, Mimosoideae), una especie con potencial agroforestal. — Ann. Inst. Biol. Univ. Nac. Autónoma de México, Ser. Bot.62: 121–132.
Hilu, K. W., Boyd, S., Felker, P., 1982: Morphological diversity and taxonomy of California mesquites (Prosopis, Leguminosae). — Madroño29: 237–254.
Holmgren, P. K., Holmgren, N. H., Barnett, L. C., 1990: Index herbariorum 1: the herbaria of the world, 8th edn. — New York: New York Botanical Garden.
Hughes, C. E., 1991:Leucaena improvement through use of lesser known species. —Leucaena Res. Reports12: 98–100.
Hunziker, J. H., Naranjo, C. A., Palacios, R. A., Poggio, L., Saidman, B. O., Burghardt, A. D., 1986: Studies on the taxonomy, hybridization and genetic variation of Argentine species ofProsopis. — Forest Ecol. Management16: 301–315.
Hutton, E. M., 1981: Natural crossing and acid tolerance in someLeucaena species. —Leucaena Res. Reports2: 2–4.
Irwin, J. A., Abbott, R. J., 1992: Morphometric and isozyme evidence for the hybrid origin of a new tetraploid radiate groundsel in York, England. — Heredity69: 431–439.
Leach, G. J., Whiffen, T., 1978: Analysis of a hybrid swarm betweenAcacia brachbotrya andA. calmifolia. — Bot. J. Linn. Soc.76: 53–69.
Liston, A., Rieseberg, L. H., Adams, R. P., Do, N., Zhu, G.-I., 1990: A method for collecting dried plant specimens for DNA and isozymes analysis and the results of a field test in Xinjiang, China. — Ann. Missouri Bot. Gard.77: 859–863.
Manly, B. F. J., 1986: Multivariant statistical methods. A primer. — London: Chapman & Hall.
McVaugh, R., 1987: Flora Novo-Galiciana,5.Leguminosae. — Ann Arbor, Michigan: University of Michigan Press.
Moffet, A. A., 1965: Genetical studies in acacias IV. Natural hybrids betweenAcacia irrorata andA. mearnsii. — Heredity20: 621–629.
—, Nixon, K., 1958: Genetical studies in acacias II. Leaf characters in hybrids between black and green wattles. — Heredity12: 199–218.
Palacios, R. A., Bravo, L. D., 1981: Hibridización natural enProsopis (Leguminosae) en la region chaqueňa argentina. Evidencias morfológicas y cromotográficas. — Darwiniana23: 3–35.
—, Brizuela, M. M., Burghardt, A. D., Zallochi, E. M., Mom, M. P., 1991:Prosopis burkartii and its possible hybrid origin. — Bull. Int. Group StudyMimosoideae 11: 38–42.
Palmer, J. D., 1986: Isolation and structural analysis of chloroplast DNA. — Meth. Enzym.118: 167–186.
—, Thompson, W. F., 1981: Rearrangements in the chloroplast genomes of mung bean and pea. — Proc. Natl. Acad. Sci. USA78: 5533–5537.
Pan, F. J., Brewbaker, J. L., 1988: Cytological studies in the genusLeucaena Benth. — Cytologia (Tokyo)53: 393–399.
Pyle, M. M., Adams, R. P., 1989: In situ preservation of DNA in plant specimens. — Taxon38: 576–581.
Rieseberg, L. H., Brunsfield, S. J., 1992: Molecular evidence and plant introgression. — InSoltis, P. S., Soltis, D. E., Doyle, J. J., (Eds): Molecular systematics of plants, pp. 151–176. — London: Chapman & Hall.
—, Beckstrom-Sternberg, S., Doan, K., 1990a:Helianthus annuus subsp.texanus has chloroplast DNA and nuclear ribosomal genes ofHelianthus debilis subsp.cucumerifolius. — Proc. Natl. Acad. Sci. USA87: 593–597.
—, Carter, R., Zona, S., 1990b: Molecular tests of the hypothesized hybrid origin of the two diploidHelianthus species (Asteraceae). — Evolution44: 1498–1511.
Ross, J. H., 1971: A note on theAcacia giraffae ×A. haematoxylon hybrid. — Bothalia10: 359–362.
- 1979: A conspectus of the AfricanAcacia species. — Mem. Bot. Survey S Africa44.
Sedgley, M., Harbard, J., Smith, R. M. M., Wickneswari, R., Griffin, A. R., 1992: Reproductive biology and interspecific hybridisation ofAcacia mangium andAcacia auriculiformis A. Cunn exBenth. (Leguminosae: Mimosoideae). — Austral. J. Bot.40: 37–48.
Shibata, K., 1962: Estudios citológicos de plantas colombianos silvestres y cultivadas. — J. Agric. Sci.8: 49–62.
Soltis, D. E., Soltis, P. S., 1989: Allopolyploid speciation inTragopogon: insights from chloroplast DNA. — Amer. J. Bot.76: 1119–1124.
—, —, 1990: Chloroplast DNA and nuclear rDNA variation: insights into autopolyploid and allopolyploid evolution. — InKawano, S., (Ed.): Biological approaches and evolutionary trends in plants, pp. 97–117. — San Diego: Academic Press.
Soltis, P. S., Soltis, D. E., 1991: Multiple origin of the allotetraploidTragopogon minus (Compositae): rDNA evidence. — Syst. Bot.16: 407–413.
—, Doyle, J. J., Soltis, D. E., 1992: Molecular data and polyploid evolution in plants. — InSoltis, P. S., Soltis, D. E., Doyle, J. J., (Eds): Molecular systematics of plants, pp. 177–201. — London: Chapman & Hall.
Sorensson, C. T., 1989: Status and mechanisms of self-incompatibility and self-compatibility inLeucaena species. — Pl. Cell Incomp. Newsl.21: 77–85.
- 1994: Seed productivity of artificial hybridizations between fifteen species inLeucaena Benth. — InLambeth, C. C., Dvorak, W. S., (Eds): IUFRO conference on tropical tree breeding, pp. 359–365. — Cali, Colombia.
—, Brewbaker, J. L., 1994: Interspecific incompatibility among fifteenLeucaena species (Mimosoideae) via artificial hybridization. — Amer. J. Bot.81: 240–247.
Stace, C. A., 1975: Hybridization and the flora of the British Isles. — London: Academic Press.
—, 1989: Plant taxonomy and biosystematics. — London: Edward Arnold.
Standley, P. C., 1922: Trees and shrubs of Mexico. — Contrib. US Natl. Herb.23.
Stebbins, G. L., 1959: The role of hybridization in evolution. — Proc. Amer. Philos. Soc.103: 231–251.
Tijo, J. H., 1948: The somatic chromosomes of some tropical plants. — Hereditas34: 135–146.
Wells, H., 1980: A distance coefficient as a hybridization index: an example usingMimulus longifolius andM. flemingii (Scrophulariaceae) from Santa Cruz Island, California. — Taxon29: 53–65.
Whitaker, T. W., Cutler, H. C., 1966: Food plants in a Mexican market. — Econ. Bot.20: 6–16.
Wilson, P., 1992: On inferring hybridity from morphological intermediacy. — Taxon41: 11–23.
Zarate, S. P., 1982: Las especies deLeucaena Benth. de Oaxaca con notas sobre la sistemática del género para México. — MSc. Thesis. Mexico City: Facultad de Ciencias, Universidad Auton. Nac. de México.
—, 1984: Taxonomic revision of the genusLeucaena Benth. from Mexico. — Bull. Int. Group StudyMimosoideae 12: 24–34.
—, 1987: Taxonomic identity ofLeucaena leucocephala (Lam.)De Wit with a new combination. — Phytologia63: 304–306.
Zimmer, E. A., Jupe, E. R., Walbot, V., 1988: Ribosomal gene structure, variation, and inheritance in maize and its ancestors. — Genetics120: 1125–1136.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hughes, C.E., Harris, S.A. The characterisation and identification of a naturally occurring hybrid in the genusLeucaena (Leguminosae: Mimosoideae). Pl Syst Evol 192, 177–197 (1994). https://doi.org/10.1007/BF00986251
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00986251