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  • Column liquid chromatography  (495)
  • Immunocytochemistry  (267)
  • evolution  (202)
  • Springer  (964)
  • American Association for the Advancement of Science
  • 1990-1994  (959)
  • 1970-1974  (5)
  • 1950-1954
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  • 1
    Electronic Resource
    Electronic Resource
    Are the oviposition traits of the South India strain of Callosobruchus maculatus maintained by natural selection? (1990)
    Springer
    Entomologia experimentalis et applicata 57 (1990), S. 143-150 
    Details
    ISSN: 1570-7458
    Keywords: Bruchidae ; Callosobruchus maculatus ; competition ; development ; evolution ; fecundity ; growth rates ; host preferences ; life tables ; mortality ; natural selection ; net reproductive rate ; oviposition traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Le taux partiel de reproduction nette (R inf0 sup* ) dépend de l'espèce de la plante sur laquelle les œufs sont pondus et du nombre de larves entrant dans la graine. La survie larvaire est réduite par 1/(le nombre de larves par graine) parce qu'une seule larve se développe dans une graine. La fécondité n'est pas modifiée par la compétition subie par les larves, la mortalité larvaire a l'effet le plus important sur R inf0 sup* . Les femelles éliminent ou réduisent la compétition larvaire en dispersant leurs œufs uniformément et font si peu d'erreurs avec une hyperdispersion que l'évolution d'un comportement plus précis n'accroîtrait R inf0 sup* que de 4% au maximum. Des femelles retournant à une distribution des œufs au hasard provoqueraient une réduction de R inf0 sup* de 25% au moins. Les légumineuses généralement cultivées dans l'Inde du Sud sont des hôtes acceptables quand elles sont présentées seules. Le choix des femelles entre 2 hôtes élève R inf0 sup* de 30% ou plus par rapport à une distribution au hasard. Les préférences les plus nettes concernent des combinaisons présentant la plus grande différence de R inf0 sup* . Les femelles qui hyperdispersent leurs œufs, choisissent leurs hôtes et évitent les pertes par compétition en empêchant que les œufs ne donnent plus de descendants que ne le ferait une ponte au hasard. Les particularités de la ponte sont variables et héritables. Les lignées se sélectionnent bien, en fonction de la dispersion de leurs œufs sur les graines, de la discrimination des plantes hôtes, et de la modulation de leur taux de ponte. La sélection naturelle maintient ces particularités du comportement d'une façon sédentaire.
    Notes: Abstract The deposition of eggs by this strain of Callosobruchus maculatus (Fab.) (Bruchidae: Coleoptera) departs from randomness in three ways; eggs are uniformly dispersed, oviposition rates drop when beans begin to carry 2 or more eggs, and there are sharp host preferences. Using random egg placement for the unspecialized condition, these traits are evaluated for their effect on a female's contributions of offspring to the next generation (R0, the net reproductive rate). The major increases in R0 result from females dispersing eggs so uniformly that larval competition is either reduced or eliminated. Females reduce their oviposition rate when the larva from an egg added to a bean is almost certain to die in competitive encounters. Host preferences and larval survival in a host are positively associated with the abundance of the host in South India. The three oviposition traits act together to give and R0 that is 25–50% than that of eggs placed at random. These traits are known to be variable and heritable, hence, the conditions necessary for natural selection are statisfied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343502
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  • 2
    Electronic Resource
    Electronic Resource
    Variation between strains of the flour beetle Tribolium castaneum in relative performance on five flours (1991)
    Springer
    Entomologia experimentalis et applicata 60 (1991), S. 173-182 
    Details
    ISSN: 1570-7458
    Keywords: Genetics ; evolution ; host adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When populations are exposed to different environments, evolutionary processes can lead either to genetically differentiated strains or to the appearance of increased generalism at the individual level. For evolution to occur, genetic variability in performance in different environments is required. Here, intraspecific genetic variation across environments was estimated in the flour beetle Tribolium castaneum (Herbst) by comparing the responses of two strains of T. castaneum to different flour types. Replicated groups from each strain were allowed to develop on either the standard whole wheat medium or on one of four novel flours (wheat, rice, corn and oat). In several of the novel flours, clear differences in mean development time or population size of one or both strains were seen relative to performance in the standard medium. Moreover, the strains differed significantly in their phenotypic responses to the flours. One strain did particularly poorly on oat flour. Reduced oviposition, reduced larval survivorship and increased larval cannibalism were examined as possible causes of the low productivity on oat flour. These three factors accounted for about 70% of the reduction in population size when this strain oviposited and developed in oat flour. The difference between these two outbred strains in response to these five flours suggests that genetic variation in resource use is present within T. castaneum and may also be present within strains and natural populations in grain storage facilities. Such variation would permit an evolutionary response to selection in multiple environments (flours). This process has agricultural implications when several types of grain are stored in a single location because it could eventually lead to the evolution of highly generalized populations of T. castaneum, an important pest of stored products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00177038
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  • 3
    Electronic Resource
    Electronic Resource
    Cockroach mating behaviors, sex pheromones, and abdominal glands (Dictyoptera: Blaberidae) (1993)
    Springer
    Journal of insect behavior 6 (1993), S. 715-735 
    Details
    ISSN: 1572-8889
    Keywords: Aphrodisiac ; cockroach ; evolution ; mating behavior ; sex pheromone ; sternal glands ; tergal glands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two chemical signals are essential in all cockroach sexual behavioral sequences: the sex pheromone released by one partner, generally the female (for long distance attraction), and an aphrodisiac sex pheromone produced exclusively by male tergal glands (for female mounting and tergal contact or “feeding” behavior). Unlike the other cockroach groups, the males of the Oxyhaloinae species produce both chemical signals: the pheromone and the aphrodisiac. The occurrence of three patterns of mating behavior (A, B, and C), the production of male sex pheromones, and the existence in the male of developed sternal and tergal glands in seven related Oxyhaloinae species, make these cockroaches a useful model for studying the evolution of mating behavior patterns. The various types of mating behavior were not classified in the previous studies by Roth and Barth. In this report, they have been named type A (female in upper position), B (male in upper position), and C (male and female end to end). In type A mating, the male tergal glands, which are licked by the females, are well developed, whereas in types B and C, there is no licking of the male's tergal secretion by the females and the tergal glands are much less developed; the aphrodisiacs secreted by the tergal glands may no longer act in this case through contact chemoreception, but through an olfactory process involving volatile components. One common sex pheromone component seems to be acetoin. I suggest that the mating behavior tends from A toward B and C during the evolutionary process with a concomitant regression of the tergal glands and changes in the aphrodisiac emission levels. The mating behavioral sequences of cockroaches (Dictyoptera) and crickets (Orthoptera) show a striking degree of similarity and are probably examples of convergent evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01201672
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  • 4
    Electronic Resource
    Electronic Resource
    Egg attendance and brooding by males of the giant water bugLethocerus medius (Guerin) in the field (Heteroptera: Belostomatidae) (1993)
    Springer
    Journal of insect behavior 6 (1993), S. 93-106 
    Details
    ISSN: 1572-8889
    Keywords: Belostomatidae ; giant water bugs ; paternal care ; eggs ; reproduction ; behavior ; brooding ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Males of the giant water bug Lethocerus medius(Guerin) typify their monobasic subfamily, the Lethocerinae, in that they do not brood eggs attached to their backs as do males of all members of the subfamily Belostomatinae. Exclusive male parental investment as expressed in the Belostomatinae is extremely rare behavior among animals, and evolution of the trait is obscure. Lethocerus mediusmales apparently remain with their mates through oviposition and are consistently found in attendance of eggs after the female has departed. This behavior may enhance paternity assurance at no cost in opportunity for polygyny. Two double clutches of eggs were found, from which we infer the potential for polygynous matings and shared parental investment. Male L. mediusbrood attended egg clutches above the surface of the water, where they may moisten them, shade them, and defend them against predation. Egg attendance/brooding by L. mediusand other Lethocerusspecies may represent a plesiomorphic state from which paternal back- brooding evolved in the Belostomatinae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01049150
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  • 5
    Electronic Resource
    Electronic Resource
    Ancient DNA: An introduction (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 521-523 
    Details
    ISSN: 1420-9071
    Keywords: Ancient DNA ; evolution ; conservation ; biology ; anthropology ; plant biology ; PCR (polymerase chain reaction)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01921719
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  • 6
    Electronic Resource
    Electronic Resource
    A novel mechanism for jumping in the indian antHarpegnathos saltator (Jerdon) (Formicidae, Ponerinae) (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 63-71 
    Details
    ISSN: 1420-9071
    Keywords: Insect ; behaviour ; high-speed cinematography ; jumping ; electrophysiology ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The Indian antHarpegnathos saltator may be unique among insects in using its jumping capacity not only as an escape mechanism but also as a normal means of locomotion, and for catching its prey in flight. High-speed cinematography used to analyse the various phases of the jump suggests thatHarpegnathos employs a novel jumping mechanism to mediate these behaviours: namely the synchronous activation of its middle and hindlegs. Electrophysiological recordings from muscles or nerves in pairs of middle and hindlegs show remarkably synchronous activity during fictive jumping, supporting the synchronous activation hypothesis.Harpegnathos is not the only ant to jump, and a cladistic analysis suggests that jumping behaviour evolved independently three times during ant evolutionary history.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01992052
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  • 7
    Electronic Resource
    Electronic Resource
    Eucaryotic codes (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 1106-1117 
    Details
    ISSN: 1420-9071
    Keywords: Genetic code ; eucaryotic cell ; evolution ; code ambiguity ; code universality ; convergence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This article is a review of the rules used by eucaryotic cells to translate a nuclear messenger RNA into a polypeptide chain. The recent observation that these rules are not identical in two species of a same phylum indicates that they have changed during the course of evolution. Possible scenarios for such changes are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01936920
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  • 8
    Electronic Resource
    Electronic Resource
    Biology of halophilic bacteria, Part II (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 1027-1036 
    Details
    ISSN: 1420-9071
    Keywords: Archaea (archaebacteria) ; extreme halophiles ; archaeol phospholipids ; archaeol glycolipids ; membrane function ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Archaebacteria (archaea) are comprised of three groups of prokaryotes: extreme halophiles, methanogens and thermoacidophiles (extreme thermophiles). Their membrane phospholipids and glycolipids are derived entirely from a saturated, isopranoid glycerol diether,sn-2,3-diphytanylglycerol (‘archaeol’) and/or its dimer, dibiphytanyldiglyceroltetraether (‘caldarchaeol’). In extreme halophiles, the major phospholipid is the archaeol analogue of phosphatidylglycerolmethylphosphate (PGP-Me); the glycolipids are sulfated and/or unsulfated glycosyl archaeols with diverse carbohydrate structure characteristic of taxons on the generic level. Biosynthesis of these archaeol-derived polar lipids occurs in a multienzyme, membrane-bound system that is absolutely dependent on high salt concentration (4 M). The highly complex biosynthetic pathways involve intermediates containing glycerol ether-linked C20-isoprenyl groups which are reduced to phytanyl groups to give the final saturated polar lipids. In methanogens, polar lipids are derived both from archaeol and caldarchaeol, and thermoacidophiles contain essentially only caldarchaeol-derived polar lipids. The function of these membrane polar lipids in maintaining the stability, fluidity and ionic properties of the cell membrane of extreme halophiles, as well as the evolutionary implications of the archaeol and caldarchaeol-derived structures will be discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01929909
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  • 9
    Electronic Resource
    Electronic Resource
    Presence of a partial urea cycle in the leech,Poecilobdella granulosa (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 729-731 
    Details
    ISSN: 1420-9071
    Keywords: Urea cycle ; leech ; botryoidal tissue ; hirudineans ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ornithine carbamoyltransferase (OCT) and arginase, but not arginine synthetase (AS), were detected in the body wall and gut tissues of the leech. The activities of these enzymes were not altered by starvation. The high activity of arginase in body wall is probably due to the association of the latter with botryoidal tissue. Hirudineans, which evolved from oligochaete ancestors, appear to have lost the citrulline-arginine segment of the urea cycle due to their ammonotelic mode of nitrogen excretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02124288
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic aspects of the hsp70 multigene family in vertebrates (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 987-1001 
    Details
    ISSN: 1420-9071
    Keywords: Hsp70 ; evolution ; gene duplication ; gene homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The family of genes encoding heat shock proteins of about 70 kDa (hsp70) in vertebrates is reviewed under genetic aspects. After a detailed description of the various hsp70 genes more general characteristics of the organization and evolution of the multigene family are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923453
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  • 11
    Electronic Resource
    Electronic Resource
    Chitin in the epidermal cuticle of a vertebrate (Paralipophrys trigloides, Blenniidae, Teleostei) (1993)
    Springer
    Cellular and molecular life sciences 49 (1993), S. 317-319 
    Details
    ISSN: 1420-9071
    Keywords: Chitin ; cuticle ; evolution ; vertebrates ; bony fish ; Blenniidae ; Paralipophrys trigoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Lectin binding, endo-chitinase binding and enzymatic degradation studies show that the epidermal cuticle of the bony fishParalipophrys trigloides (Blenniidae) is chitinous. This is the first evidence that a vertebrate species possesses a chitinous tissue. Recently aXenopus gene has been identified which has significant sequence similarity to the catalytic domain of yeast chitin synthase III, a chitin producing enzyme1,2. Taken together these two findings imply that chitin synthesis capability may be a basic vertebrate feature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01923410
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  • 12
    Electronic Resource
    Electronic Resource
    Genetic ecology: A new interdisciplinary science, fundamental for evolution, biodiversity and biosafety evaluations (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 429-437 
    Details
    ISSN: 1420-9071
    Keywords: Genetics ; ecology ; DNA-transfer ; conjugation ; transformation ; transduction ; transposons ; dormant cells ; epilithon ; microbial colonisation ; symbiosis ; virus resistance ; biosafety ; release of genes ; insults to humanity ; evolution ; biodiversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Genetic ecology is the extension of our modern knowledge in molecular genetics to studies of viability, gene expression and gene movements in natural environments like soils, aquifers and digestive tracts. In such milieux, the horizontal transfer of plasmid-borne genes between phylogenetically distant species has already been found to be much more frequent than had been expected from laboratory experience. For the study of exchanges involving chromosomally-located genes, more has to be learned about the behaviour of transposons in such environments. The results expected from studies in genetic ecology are relevant for considerations of evolution, biodiversity and biosafety. The role of this new field of research in restoring popular confidence in science and in its biotechnological applications is stressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01920741
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  • 13
    Electronic Resource
    Electronic Resource
    Basic rules of change (1990)
    Springer
    Journal for general philosophy of science 21 (1990), S. 231-257 
    Details
    ISSN: 1572-8587
    Keywords: basic rules ; change ; discipline-neutral ; evolution ; analogy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Philosophy , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Summary A small step is made in the direction of defining some general basic rules which can serve as a framework for research in several fields of the social sciences. The method of working with analogies asks for a more accurate approach. Starting from the concept of evolution in the form of a basic rule another basic rule is formulated. This rule shows what are the most important factors in long term developments and what types of development one can expect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01801037
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  • 14
    Electronic Resource
    Electronic Resource
    Evolution: Teleology or chance? (1991)
    Springer
    Journal for general philosophy of science 22 (1991), S. 133-141 
    Details
    ISSN: 1572-8587
    Keywords: evolution ; teleology ; chance ; purpose ; anthropomorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Philosophy , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Summary Revaluation of the problem of natural teleology seems an important precondition for elucidating our environmental crisis and for formulating an ‘ecological ethics’, because it calls for a recognition of an intrinsic value in nature and organisms. Therefore, it is necessary to show that the concept of natural teleology is not in contradiction with scientific theories, in particular not with the theory of evolution. In this paper I shall argue that there is a fundamental misunderstanding about the concepts of teleology and chance in modern thinking. This as a result of a radical transformation of the Aristotelian concept of teleology by Christian theologians during the Middle Ages. This confusion resulted in the rejection of teleology from evolution and in an exaggeration of the role of chance. However, not a solution for the problem of teleology is given here, but only an attempt to prove that neither the fossil-record, nor the role of chance in evolution can give adequate arguments for the negation of teleology in evolution. That is not to say that, therefore there exists teleology in evolution, but the problem of teleology in nature cannot, be solved by the scientific theory of evolution, but only be elucidated by philosophical analysis. At the end of the paper it is argued that teleology must be rather presupposed in evolution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01801253
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  • 15
    Electronic Resource
    Electronic Resource
    Life and teleology (1992)
    Springer
    Journal for general philosophy of science 23 (1992), S. 85-103 
    Details
    ISSN: 1572-8587
    Keywords: life ; teleology ; evolution ; reality ; representation ; experience
    Source: Springer Online Journal Archives 1860-2000
    Topics: Philosophy , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Summary A comprehensive definition of the phenomenon called “life” led to the addition of many dimensions to the natural sciences, and especially the conscious mental dimension. Historical attention is paid not only to those employing the natural philosophical paradigms, but also to evolutionary theories and to the Kantian teleological philosophy. The belief that science can solve the riddle of life is a category of purposal thinking. A revised version of critical teleology is essential for comprehension of life.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01801797
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  • 16
    Electronic Resource
    Electronic Resource
    Evolution of insect-plant relationships — a devil's advocate approach (1993)
    Springer
    Entomologia experimentalis et applicata 66 (1993), S. 3-12 
    Details
    ISSN: 1570-7458
    Keywords: evolution ; coevolution ; selection ; insect attack ; plant defense ; competition ; enemy free space ; chemoreception ; specialization ; plant recognition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Most hypotheses concerning the evolution of insect-plant relationships are based on the assumptions that, (1) phytophagous insects reduce plant fitness, and that (2) insect-plant relationships are the result of unconstrained selection. It can be shown, however, that there is little evidence to support these assumptions. As an alternative, it is proposed that the evolution of insect-plant relationships results primarily from autonomous evolutionary events; namely from heritable functional changes within the insects' nervous system that determine plant recognition and ultimately host plant specificity. These changes cannot be evoked by selective ecological agents. They originate from intrinsic changes (mutationssensu lato) within the insect genome. Ecological factors play a secondary role: by either supporting or preventing the establishment of the new genotype with the novel food preference.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02381541
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  • 17
    Electronic Resource
    Electronic Resource
    Evolutionary genetics of the aphid Cryptomyzus, with a preliminary analysis of the inheritance of host plant preference, reproductive performance and host-alteration (1990)
    Springer
    Entomologia experimentalis et applicata 57 (1990), S. 65-76 
    Details
    ISSN: 1570-7458
    Keywords: Cryptomyzus ; aphid ; hybridization ; host plant preference ; reproductive performance ; host-alternation ; speciation ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé C. galeopsidis Kaltenbach contient plusieurs formes qui ont différentes relations avec des plantes hôtes et des cycles distincts. Des croisements ont permis d'élucider la taxonomie de ces formes et d'étudier l'hérédité de préférences d'hôtes, des performances reproductives et de l'alternance d'hôtes. Une des formes apparaît comme une espèce distincte par suite de la valeur adaptative réduite des hybrides. Les autres formes avec alternance ou non des hôtes sont considérées comme conspécifiques et représentant deux stratégies vitales différentes. Les performances reproductives sont probablement polygéniques puisque les hybrides ont des performances intermédiaires. Les préférences d'hôtes des hybrides montrent certains degrés de dominance et semblent déterminées par quelques gènes seulement. L'alternance des hôtes est envisagée comme ayant une hérédité monofactorielle. Les conséquences sur la spéciation sont discutées.
    Notes: Abstract The aphid species Cryptomyzus galeopsidis (Kaltenbach) includes several distinct forms which have different host plant relationships and life cycles. Cross breeding was used to elucidate the taxonomic status of these forms and to investigate the inheritance of host preference, reproductive performance and host-alternation. One of the forms appeared to be a distinct species because of the reduced fitness of the hybrids. Other host-alternating and non host-alternating forms are considered conspecific and represent two life cycle strategies. Reproductive performance is probably controlled polygenically, since hybrids show an intermediate performance. Host preference in hybrids showed some degree of dominance and seemed to be determined by only a few genes. Host-alternation is presumed to be inherited monofactorially. The implications for speciation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00349596
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  • 18
    Electronic Resource
    Electronic Resource
    Extracellular localization of cathepsin D in ossifying cartilage (1973)
    Springer
    Calcified tissue international 12 (1973), S. 313-321 
    Details
    ISSN: 1432-0827
    Keywords: Cathepsin D ; Extracellular ; Ossification ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé De la cathepsine D extracellulaire d'origine principalement ostéoblastique a été démontrée par immunohistochimie dans le cartilage en voide de calcification de cultures d'os des membres d'embryons de poulet. L'intérêt de ce fait pour l'ossification est discuté.
    Abstract: Zusammenfassung Es wurde mit einer immunohistochemischen Methode gezeigt, daß extrazelluläres Kathepsin D, welches hauptsächlich aus Osteoblasten gewonnen wurde, Knorpel von kultivierten Knochen von Kükenembryonen mineralisiert. Die Bedeutung dieser Feststellung für den Mechanismus der Knochenbildung wird diskutiert.
    Notes: Abstract Extracellular cathepsin D derived mainly from osteoblasts has been demonstrated immunohistochemically in ossifying cartilage of cultured embryonic chick limb bones. The relevance of this observation to the mechanism of ossification is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013744
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  • 19
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    Opsin localization and chromophore retinoids identified within the basal brain of the lizard Anolis carolinensis (1993)
    Springer
    Journal of comparative physiology 172 (1993), S. 33-45 
    Details
    ISSN: 1432-1351
    Keywords: Photoreception ; Extraretinal Photoreceptor ; Chromophore ; Opsin ; Reptile ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Since the beginning of this century evidence has accumulated which demonstrates that non-mammalian vertebrates possess photoreceptors situated deep within the brain. While many attempts have been made to localize these sensory cells, studies have either failed or been inconclusive. In this report we have used several experimental approaches to localize the deep brain photoreceptors of the lizard Anolis carolinensis. Using 3 antibodies that bind vertebrate cone opsins, we have immunolabelled cerebrospinal fluid (CSF)-contacting neurons located at the ventricular border within the nucleus ventromedialis of the septum. Western blot analysis indicates that these antibodies recognized a single 40 kD protein in ocular, anterior brain, and pineal extracts. Immunoblots of rodent brain did not show a similar protein band. We have also identified specific retinoids associated with phototransduction (11-cis and all-trans-3,4-didehydroretinaldehyde) within anterior brain extracts. This combined data provides the most detailed analysis of deep brain photoreceptors in any vertebrate. Consequently, we feel Anolis provides an excellent model to study this unexplored sensory system of the vertebrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214713
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  • 20
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    Isolation of an egg-laying hormone-binding protein from the gonad of Aplysia californica and its localization in oocytes (1993)
    Springer
    Journal of comparative physiology 173 (1993), S. 475-483 
    Details
    ISSN: 1432-1351
    Keywords: Egg laying hormone ; Aplysia ; Binding protein ; Immunocytochemistry ; Reproductive system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A protein solubilized from a membrane preparation of the gonad of Aplysia californica has been isolated by affinity chromatography, using bag cell egg-laying hormone (ELH) as the bound ligand, and partially purified and characterized by gel electrophoresis. The protein has an apparent molecular weight of 52 kDa and consists of two disulfide-linked subunits of about 30 kDa each. The protein is glycosylated and has an acidic pI. Approximately 10–15 μg of this protein can be isolated from a single ovotestis, representing less than 1% of the total protein in the gonad; but the protein could not be detected in buccal mass or body wall, tissues which do not have apparent response to ELH. Antibodies generated against this ELH-binding protein (ELHBP) were used to localize sites in the ovotestis which might contain this molecule and thus represent targets for egg-laying hormone. Immunocytochemical results indicate that the oocytes are a rich source of this protein, since their cytoplasm was the only detectable site of immunoreactivity. Whether this binding protein represents an egg-laying hormone receptor is uncertain, but its prevalence in oocytes suggests that ELH plays a signaling role on these gametes.
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    URL: http://dx.doi.org/10.1007/BF00193520
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    Charophyta: their use in paleolimnology (1994)
    Springer
    Journal of paleolimnology 10 (1994), S. 43-52 
    Details
    ISSN: 1573-0417
    Keywords: Charophyta ; evolution ; gyrogonite morphology ; ecology-paleoecology ; Argentina ; South America
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Charophyta are common algae in limnic waters from many regions and are an interesting group from an evolutionary point-of-view, as they are believed to be related to the Chlorophyceae and land plants. Paleontological-botanical systematics are discussed, taking into consideration some new advances. Charophytes live in all types of inland waters and are sensitive to ecological change, and so they are very useful paleolimnological markers. Gaps concerning gyrogonite morphology in extant taxa and their responses to different environmental conditions must be described. This paper discusses data concerning ecological factors affecting the distribution of Argentinian Charophyta (principally distributed between 30°S and 40°S), gyrogonite morphology related to different ecological conditions, and the way that Charophyta can modify the environment.
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    URL: http://dx.doi.org/10.1007/BF00683145
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    Pleistocene Charophyta from Arroyo Perucho Verna, Province of Entre Rios, Argentina (1994)
    Springer
    Journal of paleolimnology 10 (1994), S. 53-58 
    Details
    ISSN: 1573-0417
    Keywords: Charophyta ; evolution ; gyrogonite morphology ; ecology-paleoecology ; Argentina ; South America
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The Pleistocene charophytes from Arroyo Perucho Verna, Province of Entre Ríos, were analyzed.Chara contraria Br. ex Kütz.,C. contraria var.longilinea Cáceres,C. globularis Thuill. andTolypella intricata (Trent. ex Roth.) Leonh. var.apiculata (A. Br.) Wood were described and illustrated with scanning electron microscope. The assemblage indicates fresh alkaline to slightly saline waters, not very deep, in a lentic or sometimes lotic environment. Extant assemblages provide data for this paleoecological reconstruction.
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    URL: http://dx.doi.org/10.1007/BF00683146
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    Effects of aflatoxin B1 on in vitro cultures ofNicotiana tabacum var. Samsun (1994)
    Springer
    Mycopathologia 125 (1994), S. 107-117 
    Details
    ISSN: 1573-0832
    Keywords: Aflatoxin B1 ; Immunocytochemistry ; Regeneration ; Tissue culture ; Tobacco plantlets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effects of aflatoxin B1, (0.5–25 µg ml−1) on in vitro root and shoot development in young tobacco explants were investigated. Despite an initial apparent stimulatory effect on most measured parameters at 0.5 µg ml−1 AFB1, the number of leaves, root and leaf mass per plantlet were progressively inhibited with increasing AFB1 concentration. The number of explants developing roots was reduced to 34% at the highest (25 µg ml−1) AFB1 concentration, following 3 weeks exposure to the toxin. Leaf chlorophyll content at this toxin concentration was significantly lower than that measured for control plantlets. Thin layer chromatography confirmed the absorption of AFB1 by the plantlets. Using immunocytochemical techniques, AFB1 was immunolocated predominantly in the vacuoles, the nucleus and the cytoplasm (possibly intravesicularly). The results are discussed in terms of this immunolocation within the cell.
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    URL: http://dx.doi.org/10.1007/BF01371099
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    Metabolic pathways inParacoccus denitrificans and closely related bacteria in relation to the phylogeny of prokaryotes (1992)
    Springer
    Antonie van Leeuwenhoek 61 (1992), S. 1-33 
    Details
    ISSN: 1572-9699
    Keywords: Paracoccus denitrificans ; denitrification ; methylotrophy ; cytochromec ; cytochrome oxidase ; phylogeny ; evolution ; lateral gene transfer ; nitrogen fixation ; Thiosphaera pantotropha
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Denitrification and methylotrophy inParacoccus denitrificans are discussed. The properties of the enzymes of denitrification: the nitrate-nitrite antiporter, nitrate reductase, nitrite reductase, nitric oxide reductase and nitrous oxide reductase are described. The genes for none of these proteins have yet been cloned and sequenced fromP. denitrificans. A number of sequences are available for enzymes fromEscherichia coli, Pseudomonas stutzeri andPseudomonas aeruginosa. It is concluded that pathway specificc-type cytochromes are involved in denitrification. At least 40 genes are involved in denitrification. In methanol oxidation at least 20 genes are involved. In this case too pathway specificc-type cytochromes are involved. The sequence homology between the quinoproteins methanol dehydrogenase, alcoholdehydrogenase and glucose dehydrogenase is discussed. This superfamily of proteins is believed to be derived from a common ancestor. ThemoxFJGI operon determines the structural components of methanol dehydrogenase and the associatedc-type cytochrome. Upstream of this operon 3 regulatory proteins were found. The mox Y protein shows the general features of a sensor protein and the moxX protein those of a regulatory protein. Thus a two component regulatory system is involved in both denitrification and methylotrophy. The phylogeny of prokaryotes based on 16S rRNA sequence is discussed. It is remarkable that the 16S rRNA ofThiosphaera pantotropha is identical to that ofP. denitrificans. Still these bacteria show a number of differences.T. pantotropha is able to denitrify under aerobic circumstances and it shows heterotrophic nitrification. Nitrification and heterotrophic nitrification are found in species belonging to the β-and γ-subdivisions of purple non-sulfur bacteria. Thus the occurrence of heterotrophic nitrification inT. pantotropha which belongs to the α-subdivision of purple non-sulfur bacteria is a remarkable property. FurthermoreT. pantotropha contains two nitrate reductases of which the periplasmic one is supposed to be involved in aerobic denitrification. The nitrite reductase is of the Cu-type and not of the cytochromecd 1 type as inP. denitrificans. Also the cytochromeb of theQbc complex ofT. pantotropha is highly similar to its counterpart inP. denitrificans. It is hypothesized that the differences between these two organisms which both contain large megaplasmids is due to a combination of loss of genetic information and plasmid-coded properties. The distribution of a number of complex metabolic systems in eubacteria and in a number of species belonging to the α-group of purple non sulphur bacteria is reviewed. Two possibilities to explain this haphazard distribution are considered: 1. Lateral gene transfer between distantly related micro organisms occurs frequently. 2. The eubacterial ancestors must have possessed already these properties. The distribution of these properties is due to sporadic loss during evolutionary divergence. With respect to the occurrence and frequency of lateral gene transfer two opposing views exist. According to molecular biologists lateral gene transfer occurs frequently and is very easy. Bacteria are supposed to form one large gene pool. On the other hand population geneticists have provided evidence that strong systems operate that establish reproductive isolation between diverged species and even between closely related cell lines. Data on amino acid sequences of nitrogenase proteins, cytochromesc, cytochrome oxidases, β-subunits of ATP synthase and tryptophan biosynthetic enzymes of various micro organisms were reviewed. In all these cases phylogenetic trees could be constructed based on the amino acid sequence data. In all cases this phylogenetic tree was similar to the one based on 16S rRNA homology. Only in one case evidence for the occurrence of lateral gene transfer was obtained. Therefore it is concluded that lateral gene transfer played a minor role in the distribution of complex metabolic systems among prokaryotes. It must be stressed that this does not exclude the possibility that lateral gene transfer occurred frequently in the initial stage of bacterial evolution. It is hypothesized that the appearance of nitrogen fixation, denitrification and cytochrome oxidase formation were early events in the evolution of micro organisms. Both systems are supposed to have evolved only once. Subsequently the capacity to fix nitrogen or to denitrifymust have been lost many times, just as photosynthetic capacity is supposed to have been lost many times. During evolution many systems have been lost leading to a haphazard distribution of metabolic characters among bacteria. As an example it is suggested that organisms with a respiratory chain similar to that ofEscherichia coli arose by loss of the capacity to form the Qbc complex andc-type cytochromes. The remaining systems could be controlled much better however than in the ancestral organisms.
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    The evolution of pathways for aromatic hydrocarbon oxidation inPseudomonas (1994)
    Springer
    Biodegradation 5 (1994), S. 195-217 
    Details
    ISSN: 1572-9729
    Keywords: Aromatic catabolism ; by bacteria (Pseudomonas) ; evolution ; of catabolic pathways ; hydrocarbons ; catabolism of aromatic ; Pseudomonas ; evolution of catabolism in ; oxygenases ; evolution of
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The organisation and nucleotide sequences coding for the catabolism of benzene, toluene (and xylenes), naphthalene and biphenylvia catechol and the extradiol (meta) cleavage pathway inPseudomonas are reviewed and the various factors which may have played a part in their evolution are considered. The data suggests that the complete pathways have evolved in a modular way probably from at least three elements. The commonmeta pathway operons, downstream from the ferredoxin-like protein adjacent to the gene for catechol 2,3-dioxygenase, are highly homologous and clearly share a common ancestry. This common module may have become fused to a gene or genes the product(s) of which could convert a stable chemical (benzoate, salicylate, toluene, benzene, phenol) to catechol, thus forming the lower pathway operons found in modern strains. The upper pathway operons might then have been acquired as a third module at a later stage thus increasing the catabolic versatility of the host strains.
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    URL: http://dx.doi.org/10.1007/BF00696460
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    Progressive determination of cell fates along the dorsoventral axis in the sea urchin Heliocidaris erythrogramma (1994)
    Springer
    Development genes and evolution 204 (1994), S. 62-69 
    Details
    ISSN: 1432-041X
    Keywords: Cell determination ; direct development dorsoventral axis ; echinoids ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the direct-developing sea urchinHeliocidaris erythrogramma the first cleavage division bisects the dorsoventral axis of the developing embryo along a frontal plane. In the two-celled embryo one of the blastomeres, the ventral cell (V), gives rise to all pigmented mesenchyme, as well as to the vestibule of the echinus rudiment. Upon isolation, however, the dorsal blastomere (D) displays some regulation, and is able to form a small number of pigmented mesenchyme cells and even a vestibule. We have examined the spatial and temporal determination of cell fates along the dorsoventral axis during subsequent development. We demonstrate that the dorsoventral axis is resident within both cells of the two-celled embryo, but only the ventral pole of this axis has a rigidly fixed identity this early in development. The polarity of this axis remains the same in half-embryos developing from isolated ventral (V) blastomeres, but it can flip 180° in half-embryos developing from isolated dorsal (D) blastomeres. We find that cell fates are progressively determined along the dorsoventral axis up to the time of gastrulation. The ability of dorsal half-embryos to differentiate ventral cell fates diminishes as they are isolated at progressively later stages of development. These results suggest that the determination of cell fates along the dorsoventral axis inH. erythrogramma is regulated via inductive interactions organized by cells within the ventral half of the embryo.
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    Polymorphism in the giant cocoa termite,Neotermes papua (Desneux) (1991)
    Springer
    Insectes sociaux 38 (1991), S. 263-272 
    Details
    ISSN: 1420-9098
    Keywords: Isoptera ; Kalotermitidae ; Neotermes papua ; termites ; caste differentiation ; division of labour ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The direct development ofNeotermes papua (Isoptera: Kalotermitidae) comprises four larval and three nymphal instars before the alate. The first five instars can be easily characterized. The second stage nymphs come morphologically close to the pseudergates, characterized by reduced wing buds. These nymphs can moult stationarily, i.e. with little morphological change, or to presoldiers, or proceed to the alate via the third nymphal stage. Pseudergates originate through a late and reversible deviation from the straight development to the alate. Presoldiers may derive from several stages, up to the last nymphal one; their production is subject to an inhibition by extant soldiers. This developmental schema is congruent with those described in other Kalotermitidae and the Termopsidae. By pinpointing the existence of a large pool of pluripotent individuals, in which the penultimate nymphal stage mingles with pseudergates, the present study also reveals a great similarity betweenNeotermes andProrhinotermes, and suggests that this developmental schema might be generally applicable to termites devoid of a permanent worker caste.
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    URL: http://dx.doi.org/10.1007/BF01314912
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    Molecular cloning and sequencing of 18S rDNA gene fragments from six different ant species (1993)
    Springer
    Insectes sociaux 40 (1993), S. 325-335 
    Details
    ISSN: 1420-9098
    Keywords: Formicidae ; social parasitism ; PCR ; 18 S ribosomal RNA ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The evolutionary relationship between socially parasitic ants and their hosts is still an unsolved problem. We have compared a 1.2 kb sequence of the 18 S ribosomal RNA genes of the parasitic antsDoronomyrmex kutteri, Harpagoxenus sublaevis andChalepoxenus muellerianus to the sequence of the host speciesLeptothorax acervorum andL. recedens (all subfamily Myrmicinae, tribe Leptothoracini) and to an out-group antCamponotus ligniperda (Formicinae). We found that parasitic species and the host species and alsoCamponotus ligniperda differ at less than 1% of the base positions of the 1.2 kb segment of the 18S rRNA gene. The sequences showed 80.3% identity to the 18 S ribosomal RNA genes of the beetleTenebrio molitor and only 66.5% to that of the dipteranDrosophila melanogaster.
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    Development of non-reproductive castes in the neotropical termite generaCornitermes, Embiratermes andRhynchotermes (Isoptera, Nasutitermitinae) (1992)
    Springer
    Insectes sociaux 39 (1992), S. 313-324 
    Details
    ISSN: 1420-9098
    Keywords: Isoptera ; Termitidae ; Nasutitermitinae ; caste differentiation ; phylogeny ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental pattern of the neuter castes was studied in the mandibulate nasute generaCornitermes, Embiratermes andRhynchotermes. InCornitermes walkeri, all the workers and soldiers are male. There are two larval and a single worker instar. Workers can molt into presoldiers. InEmbiratermes chagresi andRhynchotermes perarmatus, both sexes are present among the neuters. A slight sexual dimorphism (males 〉 females) is discernible among both larval instars and among workers ofE. chagresi; female workers can molt into presoldiers. InR. perarmatus, the sexual dimorphism is conspicuous from the first larval instar on. Male larvae go through two instars, then give rise to workers, which do not molt. InR. perarmatus, there is no worker stage in females, but a third larval instar, preceding the presoldier. Hypotheses are proposed as to the evolution of these caste patterns, attempting to conciliate present knowledge of Nasutitermitinae phylogeny and known evolutionary trends affecting termite caste patterns, according to the species' ecology.
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    URL: http://dx.doi.org/10.1007/BF01323951
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    Social organization in some primitive Australian ants. I.Nothomyrmecia macrops Clark (1992)
    Springer
    Insectes sociaux 39 (1992), S. 425-438 
    Details
    ISSN: 1420-9098
    Keywords: Formicidae ; Nothomyrmecia ; evolution ; sociogram ; ethogram ; recognition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Results of laboratory-based ethological studies on twoNothomyrmecia macrops colonies with individually marked workers are reported. Interactive behavioural acts constituted less than 1% of all those recorded, revealing a strong tendency by the ants not to engage in social contact. Very few workers performed queen-directed acts. They stayed near the queen, though seldom in direct contact. Division of labour was otherwise barely apparent, except that some individuals showed a propensity to guard the nest entrance. No exchange of food was observed between workers, workers and queen, or adults and larvae (apart from worker placement of prey items with larvae). A queen fed from aDrosophila carcass retrieved from the nest floor, without assistance from workers. Systematic scanned observations confirmed levels of inactivity higher than previously observed in ants (comprising almost 2/3 of recorded behavioural acts). The time budget for activities directed toward the immature stages was the same in both colonies, and fluctuated during the circadian period. Non-nestmate larvae added to worker groups were more frequently licked than nestmate larvae, but this might not involve the particular recognition of nestmateversus non-nestmate brood. These observations support the hypothesis thatNothomyrmecia is primitively eusocial, and of special significance in myrmecology.
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    The genetic code in mitochondria and chloroplasts (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 1117-1126 
    Details
    ISSN: 1420-9071
    Keywords: Genetic code ; mitochondria ; evolution ; organelles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The universal genetic code is used without changes in chloroplasts and in mitochondria of green plants. Non-plant mitochondria use codes that include changes from the universal code. Chloroplasts use 31 anticodons in translating the code; a number smaller than that used by bacteria, because chloroplasts have eliminated 10 CNN anticodons that are found in bacteria. Green plant mitochondria (mt) obtain some tRNAs from the cytosol, and genes for some other tRNAs have been acquired from chloroplast DNA. The code in non-plant mt differs from the universal code in the following usages found in various organisms: UGA for Trp, AUA for Met, AGR for Ser and stop, AAA for Asn, CUN for Thr, and possibly UAA for Tyr. CGN codons are not used byTorulopsis yeast mt. Non-plant mt, e.g. in vertebrates, may use a minimum of 22 anticodons for complete translation of mRNA sequences. The following possible causes are regarded as contributing to changes in the non-plant mt: directional mutation pressure, genomic economization, changes in charging specificity of tRNAs, loss of release factor RF2, changes in RF1, changes in anticodons, loss of lysidine-forming enzyme system, and disappearance of codons from coding sequences.
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    The fractal lung: Universal and species-related scaling patterns (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 251-254 
    Details
    ISSN: 1420-9071
    Keywords: Bronchial tree ; evolution ; fractal ; lung airway ; morphogenesis ; renormalization group theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mammalian lung exhibits features of a fractal tree: heterogeneity, self-similarity and the absence of a characteristic scale. The finite nature of the lung ultimately limits the range over which self-similarity scaling characteristics are applicable. However, generalization based on the scaling features of fractals, provides unique insight into geometric organization of anatomic structures. Furthermore, the mathematical theory of renormalization groups provides a description of the harmonically-modulated inverse power-law scaling observed for bronchial tree dimensions observed in different species. Compared to several mammalian species (dog, rat, hamster), the human lung shows marked differences in the phase of the harmonic modulation for both length and diameter measurements. These inter-species scaling differences suggest that evolutionary factors modify certain universal features of morphogenesis.
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    Endogenous synthesis of peptidoglycan in eukaryotic cells; a novel concept involving its essential role in cell division, tumor formation and the biological clock (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 921-931 
    Details
    ISSN: 1420-9071
    Keywords: Biological clock ; cell division cycle ; diaminopimelate ; evolution ; FSu ; lysine ; muramate ; muramyl dipeptide ; peptidoglycan ; sleep muropeptide ; tumor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Degradation products of peptidoglycan, the universal bacterial cell wall constituent, were previously found in animal tissues and urine. Reassessment and quantitative analysis of available data lead to an original concept, i.e. that eukaryotic cells synthesize peptidoglycan. We present a model in which this endogenously synthesized peptidoglycan is essential for the processes of eukaryotic cell division and sleep induction in animals. Genes for peptidoglycan metabolism, like those for lysine biosynthesis in plants, are probably inherited from endosymbiotic bacteria, the ancestors of mitochondria and chloroplasts. Corollaries of this concept, i.e. roles for peptidoglycan metabolism in tumor formation and in the biological clock, are supported by abundant evidence. We propose that many interactions between bacteria and eukaryotes are conditioned by their common genetic heritage.
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    Progressive determination of cell fates along the dorsoventral axis in the sea urchin Heliocidaris erythrogramma (1994)
    Springer
    Development genes and evolution 204 (1994), S. 62-69 
    Details
    ISSN: 1432-041X
    Keywords: Cell determination ; direct development dorsoventral axis ; echinoids ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the direct-developing sea urchin Heliocidaris erythrogramma the first cleavage division bisects the dorsoventral axis of the developing embryo along a frontal plane. In the two-celled embryo one of the blastomeres, the ventral cell (V), gives rise to all pigmented mesenchyme, as well as to the vestibule of the echinus rudiment. Upon isolation, however, the dorsal blastomere (D) displays some regulation, and is able to form a small number of pigmented mesenchyme cells and even a vestibule. We have examined the spatial and temporal determination of cell fates along the dorsoventral axis during subsequent development. We demonstrate that the dorsoventral axis is resident within both cells of the two-celled embryo, but only the ventral pole of this axis has a rigidly fixed identity this early in development. The polarity of this axis remains the same in half-embryos developing from isolated ventral (V) blastomeres, but it can flip 180° in half-embryos developing from isolated dorsal (D) blastomeres. We find that cell fates are progressively determined along the dorsoventral axis up to the time of gastrulation. The ability of dorsal half-embryos to differentiate ventral cell fates diminishes as they are isolated at progressively later stages of development. These results suggest that the determination of cell fates along the dorsoventral axis in H. erythrogramma is regulated via inductive interactions organized by cells within the ventral half of the embryo.
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    Chemical defense of a primitive Australian bombardier beetle (Carabidae):Mystropomus regularis (1991)
    Springer
    Chemoecology 2 (1991), S. 29-34 
    Details
    ISSN: 1423-0445
    Keywords: defensive secretion ; hot secretion ; elytral flanges ; evolution ; benzoquinones ; hydrocarbons ; bombardier beetle ; Coleoptera ; Carabidae ; Paussinae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Australian bombardier beetle,Mystropomus regularis, sprays a mixture of quinones (1,4-benzoquinone, 2-methyl-1,4-benzoquinone, 2-ethyl-1,4-benzoquinone) and hydrocarbons (principallyn-pentadecane). The defensive fluid ist generated explosively in two-chambered glands, and is ejected audibly and hot (maximal recorded temperature = 59°C).Mystropomus is a member of the paussoid lineage of bombardiers. In common with other members of the group, it has a pair of elytral flanges (flanges of Coanda), associated with the gland openings, that serve as launching guides for anteriorly-aimed ejections of spray. It is argued thatMystropomus may be the least derived of flanged paussoids, and the closest living relative of the most primitive of extant bombardiers (Metriini).
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    The egg-jelly macromolecule, a fucose sulphate glycoconjugate, originates from the accessory cells of the ovary in the sea urchin Hemicentrotus pulcherrimus (1992)
    Springer
    Development genes and evolution 201 (1992), S. 179-189 
    Details
    ISSN: 1432-041X
    Keywords: Sea urchin ; Jelly coat ; Accessory cell ; Oogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The immunocytochemical localization of the egg-jelly macromolecule, a fucose sulphate glycoconjugate (FSG) that induces the acrosome reaction in spermatozoa, was investigated in ovaries of the sea urchin Hemicentrotus pulcherrimus by use of a polyclonal antibody. The polyclonal antibody reacted with the accessory cells and oocytes in the ovarian lumen. In the accessory cells, evidence of an intense immunohistochemical reaction was observed in many globules of variable density. Products of the specific immunohistochemical reaction were frequently observed in the surface region of oocytes, at a distance from the ovarian wall. At the ultrastructural level, the polyclonal antibody was found to react with the material present in the vacuole-like structures of the globules in the accessory cells. Many gold particles, demonstrating specific immunolabelling, were associated with well-developed microvilli on the vitellogenic oocytes. In the mature oocytes, intense labelling was observed in the jelly coat but not in the vitelline coat. By contrast, oogonia and early oocytes were barely labelled. Quantitative data indicated that the extent of immunolabellings in the surface region of oocytes was very high in the vitellogenic and mature oocytes. In all cases, neither the oocyte cytoplasm nor the subcellular organelles were labelled. These results suggest that FSG is produced by the accessory cells and is deposited initially on the surface of vitellogenic oocytes for the formation of jelly. These findings may provide a new insight into the role of the accessory cells in the reproductive process of the sea urchin.
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    Seasonal variations in the production of the egg-jelly macromolecule, a fucose sulphate glycoconjugate, by the accessory cells in the ovary of the sea urchin Hemicentrotus pulcherrimus (1994)
    Springer
    Development genes and evolution 203 (1994), S. 402-410 
    Details
    ISSN: 1432-041X
    Keywords: Sea urchin ; Egg jelly ; Ovary ; Development ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the sea urchin Hemicentrotus pulcherrimus, the egg-jelly macromolecule, a fucose sulphate glycoconjugate (FSG) that induces the acrosome reaction in spermatozoa, originates from the accessory cells in the ovary. In the present study we examined the seasonal variations in the distribution of FSG in the ovary by immunocytochemistry with a polyclonal antibody. An enzyme-linked immunosorbent assay indicated that FSG was present in supernatants of extracts of ovaries throughout the development of the ovary. However, the immunohistochemical study showed that there are marked seasonal changes in the distribution of FSG in ovaries. The polyclonal antibody reacted strongly with globules of accessory cells before the beginning of the breeding season (August to December). During the breeding season (February to April), the immunohistochemical reaction was found on the surface of oocytes but was weak in the accessory cells. At the ultrastructural level, the antibody reacted with globules of variable density in accessory cells. Intense immunolabelling was observed in the vacuole-like structures of the globules. Sometimes, products of the specific immunocytochemical reaction were found in the Golgi apparatus in these globules. Quantitative examination indicated that FSG was actively produced by the accessory cells from the late non-breeding season to the pre-breeding season. These results suggest that there are marked seasonal variations in the production of FSG by the accessory cells in the sea urchin ovary. These findings also provide new evidence that accessory cells exhibit dynamic changes during the reproductive process in the sea urchin.
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    URL: http://dx.doi.org/10.1007/BF00188689
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    Implications of toxins in the ecology and evolution of plant pathogenic microorganisms: Bacteria (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 791-803 
    Details
    ISSN: 1420-9071
    Keywords: Phytotoxins ; ecology ; phylogeny ; evolution ; biosynthesis ; coronatine ; phaseolotoxin ; rhizobitoxine ; syringomycin ; syringotoxin ; syringostatin ; tabtoxin ; tagetitoxin ; tropolone ; fireblight toxin ; thaxtomin ; 3-methylthiopropionic acid ; carboxylic acids ; Pseudomonas ; Xanthomonas ; Xanthomonas ; Streptomyces ; Erwinia ; Bradyrhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract This review attempts to rationalise what is known about bacterial phytotoxins and associate it with the ecology and possible evolution of the producing organisms. Study of non-toxin producing variants gives insight into the ecological role of the toxin. Elucidation of chemical structures of phytotoxins has shown that many exist as families of analogous compounds. Studies on the variation of chemical structures and how they are distributed across species and genera can lead to development of hypotheses on evolutionary relationships. Knowledge on biosynthetic pathways to tosins allows recognition of specific enzymatic steps involved in developing the characteristic features of the structures. Phytotoxins often have a potent biochemical activity, and in some cases the producing organism has associated mechanisms to prevent action of the toxin upon itself; in such cases toxigenesis is clearly not a chance event. The various aspects of bacterial toxigenesis indicate that bacterial phytotoxins are special secondary metabolic products that play beneficial roles to the producing organisms in their various ecological niches.
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    URL: http://dx.doi.org/10.1007/BF01922459
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    The Light Green Cells ofLymnaea: A neuroendocrine model system for stimulus-induced expression of multiple peptide genes in a single cell type (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 464-473 
    Details
    ISSN: 1420-9071
    Keywords: Molluscan insulin-related peptides ; schistosomin ; neuropeptide gene family ; generation of neuropeptide diversity ; stimulus-dependent expression ; information-handling capacity ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We review recent experiments showing that the cerebral neuroendocrine Light Green Cells (LGCs) of the freshwater snail,Lymnaea stagnalis, express a family of distinct though related molluscan insulin-related peptide (MIP) genes. The LGCs are involved in the regulation of a wide range of interrelated life processes associated with growth, (energy) metabolism and reproduction. We consider the mechanism of generation of diversity among MIPs, and present evidence that conditions with distinct effects on growth, metabolism and reproduction also can induce distinct patterns of expression of the MIP and schistosomin genes. The stimulus-dependent expression of multiple neuropeptide genes enormously increases the adaptive potential of a peptidergic neuron. We suggest that this contributes significantly to the information-handling capacity of the brain.
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    URL: http://dx.doi.org/10.1007/BF01928165
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    Parental care in terrestrial gastropods (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 5-14 
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    ISSN: 1420-9071
    Keywords: parental investment ; juvenile survival ; evolution ; gastropods ; molluscs ; ovoviviparity ; viviparity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Parental care in terrestrial gastropods includes the of oviposition sites, production of large, heavily-yolked eggs supplied with calcium carbonate, provisioning of hatchings with eggs in specis with facultative sibling cannibalism, egg retention, and ovoviviparity. Evidence for true viviparity is scarce in terrestrial gastropods, as it is for postlaying care of eggs, though external egg carrying on the shell occurs in a few species. Care of young has not been observed in any terrestrial gastropod species. Provisioning of eggs with nutrients and calcium carbonate might be the most common form of parental investment. Ovoviviparity allows terrestrial gastropods to persist in habitats otherwise unsuitable for oviparous species (e.g. exposed rock walls). An interspecific comparison demonstrates that egg-retaining and ovoviviparous species produce smaller clutches than oviparous species and suggests a cost of parental care.
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    Immunocytochemical identification of androgen receptor in mouse osteoclast-like multinucleated cells (1994)
    Springer
    Calcified tissue international 54 (1994), S. 325-326 
    Details
    ISSN: 1432-0827
    Keywords: Androgen Receptor ; Osteoclast ; Mouse ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Expression of androgen receptor (AR) in mouse osteoclast-like multi-nucleated cells (OCs) was examined with immunocytochemical techniques. Murine OCs were obtained by co-culturing mouse osteoblastic cells and bone marrow cells. Three preparations of polyclonal anti-AR antibody which were raised in rabbit against different parts of the human AR were employed for the experiments. Specific staining for AR was demonstrated in the nuclei and the perinuclear area of mouse OCs. This is the first report demonstrating the presence of AR in osteoclast-like cells.
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    Expression of growth hormone receptor by immunocytochemistry in rat molar root formation and alveolar bone remodeling (1992)
    Springer
    Calcified tissue international 50 (1992), S. 541-546 
    Details
    ISSN: 1432-0827
    Keywords: Growth hormone ; Growth hormone receptor ; Odontogenesis ; Bone remodeling ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Growth hormone (GH) may regulate tooth formation and bone remodeling associated with tooth eruption. This study reports the distribution of growth hormone receptor/binding protein in developing rat molars and adjacent alveolar bone by immunocytochemistry using well-characterized anti-growth hormone receptor monoclonal antibodies. These tissues represent an excellent model for studying the ontogenic changes that occur in odontogenic and osteogenic cells, as these cells are found in linear arrays displaying the various stages of morphological and functional differention, and differentiated function. Immunoreactivity was first seen in precementoblasts in contact with the epithelial root sheath, and preodontoblasts. However, growth hormone receptor immunoreactivity was associated primarily with the cytoplasm of odontogenic and osteogenic cells forming their respective matrices. Thus, cementoblasts and odontoblasts at sites of new matrix formation showed intense immunoreactivity whereas cementocytes and mature odontoblasts at later stages of tooth development were nonreactive. Osteoblasts engaged in intramembranous ossification in the alveolar bone were positive, although osteocytes and endosteal cells were immunonegative. Osteoclasts at sites of alveolar bone remodeling resorption were also immunopositive. These patterns of receptor expression parallel the ontogenic sequences of odontogenic and osteogenic cells and suggest that GH promotes the functional state of these cells. Our results also imply that GH may influence differentiation or differentiated functions associated with odontogenesis, osteogenesis, and bone remodeling independent of systemic insulin-like GF-I.
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    Microfilaments (F-actin) in generative cells and sperm: an evaluation (1992)
    Springer
    Sexual plant reproduction 5 (1992), S. 89-100 
    Details
    ISSN: 1432-2145
    Keywords: Actin ; Cytoskeleton ; Generative cell ; Immunocytochemistry ; Microtubule ; Mitosis ; Phragmoplast ; Pollen ; Rhodamine phalloidin ; Sperm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Disagreement has arisen over the presence of actin-containing microfilaments (Mfs) in angiosperm generative cells and sperm (GSP). In order to address this issue, we subjected GSP of Tradescantia virginiana, Nicotiana tabacum and Rhododendron laetum to a series of localizations using different antiactins, rhodamine phalloidin and antimyosin. Coordinate staining with antitubulin and Hoechst 33258 defined the status of the microtubule (Mt) cytoskeleton and stages of generative cell division. Additional experiments utilized cytochalasin D (CD). In no instance could Mfs be detected in GSP of the three species. Instead, Mfs seen at the periphery of GSP appear to be continuous with vegetative Mfs and thus are in the vegetative cytoplasm. Mfs are not seen in the constriction zone of dividing T. virginiana generative cells, nor are they indicated in the phragmoplast of N. tabacum and R. laetum. Myosin localizations reveal punctate staining in the vegetative cytoplasm and a thin line of fluorescence around the the outside of the generative cell. While CD seems to delay generative cell division, cytokinesis still takes place. CD-induced Mf fragments are evident in the vegetative cytoplasm but not in GSP. The weight of evidence therefore indicates that GSP do not contain Mfs. The implications of this conclusion for the behavior of GSP and the mechanism of cytokinesis in dividing generative cells are considerable.
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    Circadian photoreception in the retinally degenerate mouse (rd/rd) (1991)
    Springer
    Journal of comparative physiology 169 (1991), S. 39-50 
    Details
    ISSN: 1432-1351
    Keywords: Photoreception ; Retinally degenerate ; Mouse ; Circadian ; Rods ; Cones ; 11-cis retinaldehyde ; Immunocytochemistry ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have examined the effects of light on circadian locomotor rhythms in retinally degenerate mice (C57BL/6J mice homozygous for the rd allele: rd/rd). The sensitivity of circadian photoreception in these mice was determined by varying the irradiance of a 15 min light pulse (515 nm) given at circadian time 16 and meauring the magnitude of the phase shift of the locomotor rhythm. Experiments were performed on animals 80 days of age. Despite the loss of visual photoreceptors in the rd/rd retina, animals showed circadian responses to light that were indistinguishable from mice with normal retinas (rd/+ and +/+). While no photoreceptor outersegments were identified in the retina of rd/rd animals (80–100 days of age), we did identify a small number of perikarya that were immunoreactive for cone opsins, and even fewer cells that contained rod opsin. Using HPLC, we demonstrated the presence and photoisomerization of the rhodopsin chromophore 11-cis retinaldehyde. The rd/rd retinas contained about 2% of 11-cis retinaldehyde found in +/+ retinas. We have yet to determine whether the opsin immunoreactive perikarya or some other unidentified cell type mediate circadian light detection in the rd/rd retina.
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    Periplasmic location of nitrous oxide reductase and its apoform in denitrifying Pseudomonas stutzeri (1992)
    Springer
    Archives of microbiology 157 (1992), S. 218-222 
    Details
    ISSN: 1432-072X
    Keywords: Denitrification ; N2O reductase ; Nitrite reductase ; Immunocytochemistry ; Localization ; Two-dimensional electrophoresis ; Cell fractionation ; Pseudomonas stutzeri
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immunogold labelling techniques on ultrathin sections of low temperature embedded cells yielded evidence for the periplasmic location of the respiratory enzymes N2O reductase and nitrite reductase (cytochrome cd 1) in Pseudomonas stutzeri strain ZoBell. Cell fractionation by spheroplast preparation and two-dimensional electrophoresis showed the absence of a membrane association of these enzymes. Immunocytochemical localization of N2O reductase in a mutant strain deficient in the chromophore of N2O reductase showed the gold label at the cell periphery, indicating that the copper chromophore processing takes place after export of this protein's apoform.
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    The origin of life — A task in molecular engineering (1992)
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    Molecular engineering 1 (1992), S. 377-399 
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    ISSN: 1572-8951
    Keywords: Origin of life ; molecular engineering ; biology ; evolution ; genetic code ; translation machine ; self instruction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In attempting to understand how life originated, we search for a detailed sequence of experimentally testable physico-chemical steps in an appropriately structured system. This goal is approached in two stages. First we search for the organizational structure of processes leading to systems with the basic features of living organisms. This is an engineering problem: finding a certain construct by taking care of logical requirements and restrictions from physics. Then we face this construct with the chemical and geophysical reality, and this leads to the view that systems with the essential features of early living organisms evolve following a distinct pathway. Energy supply and the presence of a particular structure in space and time are necessary to induce and drive the processes triggered by stochastic events; but if these particular conditions are given, the broad line of the evolutionary processes is determined by logical requirements and by chemical and geophysical constrains and invariants. The genetic machinery considered to evolve in this manner agrees, in its organizational structure and in many details, with the actual genetic machinery of biosystems. A surprising simplicity and transparency is observed in the logic of the basic processes involved in the origin of life. In the present view, the processes leading to the origin of life begin in a very particular, highly structured, small region where the relevant chemistry can be quite different from overall prebiotic chemistry. Energy-rich compounds are present in ample amounts and a succession of physico-chemical processes, which are per se thermodynamically allowed, takes place. This is in contrast to popular views that the origin of life is connected with fundamental thermodynamic questions related to the problem of getting order out of chaos.
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    The evolution of the mammalian Y chromosome (1990)
    Springer
    Behavior genetics 20 (1990), S. 109-126 
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    ISSN: 1573-3297
    Keywords: Y chromosome ; DNA ; mammals ; evolution ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract There is a predominant theory for the evolution of the mammalian Y chromosome. This theory hypothesizes that genes for sex determination and male-specific traits, as well as sequences for X-Y meiotic pairing, are conserved on the mammalian Y chromosome across all lineages and that all other Y chromosomal genes or sequences have been or will be lost in each mammalian lineage. There are effects of mouse Y chromosomal genes on behaviors and other traits that are not male specific. Under the predominant theory, these Y chromosomal genes could be the same as the conserved genes for sex determination or malespecific traits, or they could be genes that have been lost from the Y chromosomes of other mammalian lineages and that will eventually be lost from the Y chromosome of the rodent lineage. Recently, the evolution of the primate and rodent Y chromosomes has been studied at the DNA level. These studies are summarized and reviewed in this article. The findings of these studies are not fully consistent with the predominant theory for the evolution of the mammalian Y chromosome. Also, they imply that there are other possibilities for the phylogenetic history of Y chromosomal genes of mice with effects on behavior. These are that Y chromosomal genes with effects on mouse behaviors or other traits could be conserved genes other than those for sex determination or malespecific traits or that they could be novel genes on the Y chromosome of the rodent orMus lineage.
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    Evolution of the glycophorin gene family in the hominoid primates (1990)
    Springer
    Biochemical genetics 28 (1990), S. 209-222 
    Details
    ISSN: 1573-4927
    Keywords: glycophorin A ; glycophorin B ; evolution ; primates ; chimpanzee ; gorilla ; orangutan ; gibbon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Analysis of nucleotide sequences of the human glycophorin A (GPA) and glycophorin B (GPB) genes has indicated that the GPA gene most closely resembles the ancestral gene, whereas the GPB gene likely arose from the GPA gene by homologous recombination. To study the evolution of the glycophorin gene family in the hominoid primates, restricted DNA on Southern blots from man, pygmy chimpanzee, common chimpanzee, gorilla, orangutan, and gibbon was probed with cDNA fragments encoding the human GPA and GPB coding and 3′-untranslated regions. This showed the presence in all of the hominoid primates of at least one GPA-like gene. In addition, at least one GPB-like gene was detected in man, both chimpanzee species, and gorilla, strongly suggesting that the event that produced the GPB gene occurred in the common ancestor of man-chimpanzee-gorilla. An unexpected finding in this study was the conservation ofEcoRI restriction sites relative to those of the other four enzymes used; the significance of this observation is unclear, but raises the question of nonrandomness ofEcoRI restriction sites in noncoding regions. Further analysis of the evolution of this multigene family, including nucleotide sequence analysis, will be useful in clarification of the evolutionary relationships of the hominoid primates, in correlation with the structure and function of the glycophorin molecules, and in assessment of the role of evolution in the autogenicity of glycophorin determinants.
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    Poly(ADP-ribose) polymerase: Structural conservation among different classes of animals and its implications (1994)
    Springer
    Molecular and cellular biochemistry 138 (1994), S. 25-32 
    Details
    ISSN: 1573-4919
    Keywords: NAD ; evolution ; polymerase chain reaction ; zinc finger ; leucine zipper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Poly(ADP-ribose) polymerase cDNAs have been isolated from different classes of animals. Cloning of genes from lower eukaryotes has allowed us to investigate directly the biological functions of poly(ADP-ribosyl)ationin vivo. The conservation of specific regions among mammals, chicken,Xenopus laevis, andDrosophila melanogaster reveals the essential structural elements required for recognition of breaks in DNA and for catalytic activity. Cys, His and basic residues in the zinc-finger consensus region are conserved. The carboxyl terminal region corresponding to an NAD-binding domain is strongly conserved. The dinucleotide-binding consensus sequence and β1-αA-β2, Rossmann fold structure, and β-sheet structures are completely conserved from mammals to insect. InDrosophila, a putative leucine-zipper motif has been identified, and other poly(ADP-ribose) polymerases also contain an α-helical, amphipathic structure in the auto-modification domain. In this article, we review the recent structural analyses of the functional domains of poly(ADP-ribose) polymerase in phylogenetically divergent species, and discuss the implications of structural conservation for its biological functions.
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    The gene coding for glutamate dehydrogenase inDrosophila melanogaster (1990)
    Springer
    Biochemical genetics 28 (1990), S. 337-346 
    Details
    ISSN: 1573-4927
    Keywords: glutamate dehydrogenase ; Drosophila melanogaster ; gene expression ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We have isolated theDrosophila melanogaster locus coding forl-glutamate dehydrogenase (EC 1.4.1.3) by virtue of its similarity to the corresponding human gene. There is only one copy of this gene in the fruit fly genome, located on the right arm of chromosome 3 (95D1-4). The transcript includes at least one large intron and matures to a ∼2.4-kb-long polyadenylated RNA whose expression is under developmental control.
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    Multiple restriction fragment length polymorphisms associated with the Vc determinant of the MN blood group-related chimpanzee V-A-B-D system (1990)
    Springer
    Biochemical genetics 28 (1990), S. 223-231 
    Details
    ISSN: 1573-4927
    Keywords: restriction fragment length polymorphisms ; glycophorin A ; glycophorin B ; MN blood group ; V-A-B-D blood group ; Vc antigen ; chimpanzee ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Twelve restriction fragment length polymorphisms (RFLPs) were detected in common chimpanzee using two restriction enzymes (HindIII andMspI) and four DNA probes to the coding regions of the human glycophorin A (GPA) and glycophorin B (GPB) genes and their 3′-untranslated regions. Seven RFLPs correlated with red cell expression of the Vc determinant of the MN blood group-related V-A-B-D system and five RFLPs correlated with nonexpression of this antigen. Animals heterozygous for theV allele that encodes the Vc determinant had all 12 polymorphic restriction fragments and appeared to show reduced intensity of probe hybridization to these fragments, consistent with the presence of aV and a non-V allele. No RFLPs were detected withEcoRI,SstI, orBamHI, in spite of the relatively large segment of DNA (at least 20 kb) involved in the polymorphisms. The RFLPs were chimpanzee specific and were not found in man, gorilla, orangutan, or gibbon. Multiple RFLPs distinguishing primate species are rare and may be useful markers for molecular evolution.
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    Sequence homology and structure predictions of the creatine kinase isoenzymes (1994)
    Springer
    Molecular and cellular biochemistry 133-134 (1994), S. 245-262 
    Details
    ISSN: 1573-4919
    Keywords: creatine kinase ; arginine kinase ; protein sequence comparison ; evolution ; CK framework ; ‘diagnostic boxes’ ; secondary structure prediction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Comparisons of the protein sequences and gene structures of the known creatine kinase isoenzymes and other guanidino kinases revealed high homology and were used to determine the evolutionary relationships of the various guamidino kinases. A ‘CK framework’ is defined, consisting of the most conserved sequence blocks, and ‘diagnostic boxes’ are identified which are characteristic for anyone creatine kinase isoenzyme (e.g. for vertebrate B-CK) and which may serve to distinguish this isoenzyme from all others (e.g. from M-CKs and Mi-CKs). Comparison of the guanidino kinases by near-UV and far-UV circular dichroism further indicates pronounced conservation of secondary structure as well as of aromatic amino acids that are involved in catalysis.
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    Evolution of behavioral responses to sex pheromone in mutant laboratory colonies ofTrichoplusia ni (1994)
    Springer
    Journal of chemical ecology 20 (1994), S. 231-238 
    Details
    ISSN: 1573-1561
    Keywords: Trichoplusia ni ; Lepidoptera ; Noctuidae ; sex pheromone ; behavior ; evolution ; sexual selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male cabbage looper moths,Trichoplusia ni, from two colonies in which all females express an abnormal sex pheromone production phenotype were evaluated in a laboratory wind tunnel for upwind flight responses to the normal and abnormal sex pheromones. The abnormal sex pheromone blend consisted of 20 times as much (Z)-9-tetradecenyl acetate and 30-fold less (Z)-5-dodecenyl acetate compared to the normal pheromone blend. Initially, these males exhibited poor behavioral responses to the abnormal sex pheromone and maximum responses to the normal pheromone blend, indicating that there was no linkage between signal production and response. After 49 generations of laboratory rearing, males from the mutant colonies maintained good responses to the normal pheromone and increased their behavioral response to the abnormal sex pheromone to the same levels as for the normal pheromone. Over the same period, normal males maintained their preference for the normal pheromone. These results indicated that evolution had occurred in mutant colonies in favor of greater male responsiveness to the abnormal sex pheromone, resulting in the broadening of the response spectrum to pheromone blend ratios. This evolution presumably resulted from a mating advantage to those males that did not discriminate against mutant-type females in the mutant colonies.
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    The gene coding for glutamate dehydrogenase inDrosophila melanogaster (1990)
    Springer
    Biochemical genetics 28 (1990), S. 337-346 
    Details
    ISSN: 1573-4927
    Keywords: glutamate dehydrogenase ; Drosophila melanogaster ; gene expression ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We have isolated theDrosophila melanogaster locus coding forl-glutamate dehydrogenase (EC 1.4.1.3) by virtue of its similarity to the corresponding human gene. There is only one copy of this gene in the fruit fly genome, located on the right arm of chromosome 3 (95D1-4). The transcript includes at least one large intron and matures to a ∼2.4-kb-long polyadenylated RNA whose expression is under developmental control.
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    Personality and reproductive fitness (1990)
    Springer
    Behavior genetics 20 (1990), S. 563-568 
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    ISSN: 1573-3297
    Keywords: personality ; fertility ; family size ; fitness ; extraversion ; neuroticism ; impulsivity ; genetic architecture ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract The relationship between reproductive success (number of biological children) and personality was explored in 1101 postmenopausal females from the Australian twin registry. The quadratic response surface relating fitness to extraversion (E) and neuroticism (N) showed a saddle point at intermediate levels of E and N. Selection was shown to be stabilizing, i.e.,having an intermediate optimum, along the axis low E, low N-high E, high N and more mildly disruptive, having greater fitness in the extremes, along the axis low N, high E-high N, low E. Neither dimension of personality considered by itself showed a significant linear or quadratic relationship to reproductive success. Sections through the fitness surface, however, show selection tends to favor high neuroticism levels in introverts and low neuroticism levels in extroverts.
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    Nucleotide sequence ofatpB,rbcL,trnR,dedB andpsaI chloroplast genes from a fernAngiopteris lygodiifolia: a possible emergence of Spermatophyta lineage before the separation of Bryophyta and Pteridophyta (1992)
    Springer
    Plant molecular biology 18 (1992), S. 79-82 
    Details
    ISSN: 1573-5028
    Keywords: Angiopteris lygodiifolia ; atpB ; chloroplast genome ; evolution ; rbcL ; trnR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fernAngiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained theatpB,rbcL,trnR-CCG,dedB andpsaI genes. Comparisons of the deduced amino acid and nucleotide sequences of these genes from the three plant groups indicate thatAngiopteris sequences are more closely related to those of Bryophyta species (85% identity on average) than to those of seed plants (76% identity on average), supporting a hypothesis that the Bryophyta and Pteridophyta diverged more recently from one another than their common progenitor diverged from that of the Spermatophyta.
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    URL: http://dx.doi.org/10.1007/BF00018458
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    The activation process of Arabidopsis thaliana A1 gene encoding the translation elongation factor EF-1α is conserved among angiosperms (1992)
    Springer
    Plant molecular biology 18 (1992), S. 1083-1089 
    Details
    ISSN: 1573-5028
    Keywords: translation elongation factor genes ; promoter analysis ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Arabidopsis thaliana, the activation process of the A1 EF-1α gene depends on several elements. Using the GUS reporter gene, transient expression experiments have shown that mutations of upstream cis-acting elements of the A1 promoter, or the deletion of an intron located within the 5′ non-coding region, similarly affect expression in dicot or monocot protoplasts. The results reported here strongly suggest that this 5′ intron is properly spliced in Zea mays. We show that two trans-acting factors, specifically interacting with an upstream activating sequence (the TEF 1 box), are present in nuclear extracts prepared from A. thaliana, Brassica rapa, Nicotiana tabacum and Z. mays. In addition, a DNA sequence homologous to the TEF 1 box, found at approximately the same location within a Lycopersicon esculentum EF-1α promoter, interacts with the same trans-acting factors. Homologies found between the A. thaliana and L. esculentum TEF 1 box sequences have allowed us to define mutations of this upstream element which affect the interaction with the corresponding trans-acting factors. These results support the notion that the activation processes of A. thaliana EF-1α genes have been conserved among angiosperms and provide interesting data on the functional structure of the TEF 1 box.
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    URL: http://dx.doi.org/10.1007/BF00047711
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    A rice (Oryza sativa L.) cDNA encodes a protein sequence homologous to the eukaryotic ribosomal 5S RNA-binding protein (1993)
    Springer
    Plant molecular biology 23 (1993), S. 409-413 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; cloning ; rice ; L5 ; ribosomal 5 S RNA-binding protein ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rice (Oryza sativa L.) cDNA clone coding for the cytoplasmic ribosomal protein L5, which associates with 5 S rRNA for ribosome assembly, was cloned and its nucleotide sequence was determined. The primary structure of rice L5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. These include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three repeated amino acid sequences; the conservation of glycine residues. This protein was identified as the nuclear-encoded cytoplasmic ribosomal protein L5 of rice by sequence similarity to other eucaryotic ribosomal 5 S RNA-binding proteins of rat, chicken, Xenopus laevis, and Saccharomyces cerevisiae. Rice L5 shares 51 to 62% amino acid sequence identity with the homologues. A group of ribosomal proteins from archaebacteria including Methanococcus vanniellii L18 and Halobacterium cutirubrum L13, which are known to be associated with 5 S rRNA, also related to rice L5 and the other eucaryotic counterparts, suggesting an evolutionary relationship in these ribosomal 5 S RNA-binding proteins.
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    URL: http://dx.doi.org/10.1007/BF00029016
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    A 16 kb small single-copy region separates the plastid DNA inverted repeat of the unicellular red alga Cyanidium caldarium: physical mapping of the IR-flanking regions and nucleotide sequences of the psbD-psbC, rps16, 5S rRNA and rpl21 genes (1992)
    Springer
    Plant molecular biology 19 (1992), S. 1001-1010 
    Details
    ISSN: 1573-5028
    Keywords: Cyanidium caldarium Geitler ; evolution ; Galdieria sulphuraria ; inverted repeat ; plastid DNA ; psbD-psbC operon ; red algae ; 5S rRNA ; rpl21 ; rps16
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The four inverted repeat (IR) flanking regions of the Cyanidium caldarium plastid DNA were cloned. Southern blotting, transcript and sequence analyses of the border regions revealed the psbD-psbC operon and the rps16 gene within the large single-copy region upstream of the 16S rDNA gene and the rpl21 gene downstream of the 5S rDNA within the 16 kb small single-copy region. The size of the IR is ca. 5 kb. The nucleotide sequences of the psbD-psbC, rps16, rpl21 and 5S rRNA genes with the corresponding alignments and physical maps of the regions are presented. Northern analysis revealed a less complex psbD-psbC transcription pattern than has been found in higher plants. Comparisons to other red algal data point to structural diversity within red algal plastid DNA.
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    URL: http://dx.doi.org/10.1007/BF00040531
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    Comparison of regions coding for tRNAHis genes of mitochondrial and chloroplast DNA in sunflower: a proposal concerning the classification of ‘CP-like’ tRNA genes (1992)
    Springer
    Plant molecular biology 20 (1992), S. 1-4 
    Details
    ISSN: 1573-5028
    Keywords: higher plants ; sunflower ; chloroplast genome ; mitochondrial genome ; tRNAHis genes ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029143
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    Genomic structure, expression and evolution of the alfalfa aspartate aminotransferase genes (1994)
    Springer
    Plant molecular biology 25 (1994), S. 387-399 
    Details
    ISSN: 1573-5028
    Keywords: aspartate aminotransferase ; gene structure ; nodule ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic clones encoding two isozymes of aspartate aminotransferase (AAT) were isolated from an alfalfa genomic library and their DNA sequences were determined. The AAT1 gene contains 12 exons that encode a cytosolic protein expressed at similar levels in roots, stems and nodules. In nodules, the amount of AAT1 mRNA was similar at all stages of development, and was slightly reduced in nodules incapable of fixing nitrogen. The AAT1 mRNA is polyadenylated at multiple sites differing by more than 250 bp. The AAT2 gene contains 11 exons, with 5 introns located in positions identical to those found in animal AAT genes, and encodes a plastid-localized isozyme. The AAT2 mRNA is polyadenylated at a very limited range of sites. The transit peptide of AAT2 is encoded by the first two and part of the third exon. AAT2 mRNA is much more abundant in nodules than in other organs, and increases dramatically during the course of nodule development. Unlike AAT1, expression of AAT2 is significantly reduced in nodules incapable of fixing nitrogen. Phylogenetic analysis of deduced AAT proteins revealed 4 separate but related groups of AAT proteins; the animal cytosolic AATs, the plant cytosolic AATs, the plant plastid AATs, and the mitochondrial AATs.
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    URL: http://dx.doi.org/10.1007/BF00043868
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    Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach (1993)
    Springer
    Plant molecular biology 21 (1993), S. 331-340 
    Details
    ISSN: 1573-5028
    Keywords: cDNA sequences ; evolution ; fructose-1,6-bisphosphate aldolase ; Spinacia oleracea ; transit peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the two enzymes from spinach share only 54% homology on their amino acid level whereas the homology of the cytosolic enzyme of spinach with the known sequences of cytosolic aldolases of maize, rice and Arabidopsis range from 67 to 92%. The sequence of the chloroplastic enzyme includes a stroma-targeting N-terminal transit peptide of 46 amino acid residues for import into the chloroplast. The transit peptide exhibits essential features similar to other chloroplast transit peptides. Southern blot analysis implies that both spinach enzymes are encoded by single genes.
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    Sorghum phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolution (1993)
    Springer
    Plant molecular biology 21 (1993), S. 487-502 
    Details
    ISSN: 1573-5028
    Keywords: C4 metabolism ; evolution ; GC content ; gene family ; PEPC ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Although housekeeping functions have been shown for the phosphoenolpyruvate carboxylase (EC 4.1.1.31, PEPC) in plants and in prokaryotes, PEPC is mainly known for its specific role in the primary photosynthetic CO2 fixation in C4 and CAM plants. We have shown that in Sorghum, a monocotyledonous C4 plant, the enzyme is encoded in the nucleus by a small multigene family. Here we report the entire nucleotide sequence (7.5 kb) of the third member (CP21) that completes the structure of the Sorghum PEPC gene family. Nucleotide composition, CpG islands and GC content of the three Sorghum PEPC genes are analysed with respect to their possible implications in the regulation of expression. A study of structure/function and phylogenetic relationships based on the compilation of all PEPC sequences known so far is presented. Data demonstrate that (1) the different forms of plant PEPC have very similar primary structures, functional and regulatory properties, (2) neither apparent amino acid sequences nor phylogenetic relationships are specific for the C4 and CAM PEPCs and (3) expression of the different genes coding for the Sorghum PEPC isoenzymes is differently regulated (i.e. by light, nitrogen source) in a spatial and temporal manner. These results suggest that the main distinguishing feature between plant PEPCs is to be found at the level of genes expression rather than in their primary structure.
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    URL: http://dx.doi.org/10.1007/BF00028806
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    Genomic rearrangement of the α-globin gene complex during mammalian evolution (1993)
    Springer
    Biochemical genetics 31 (1993), S. 473-484 
    Details
    ISSN: 1573-4927
    Keywords: hydroxyacyl glutathione hydrolase (glyoxalase II) ; chromosome mapping ; evolution ; Mus musculus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In man, the gene for hydroxyacyl glutathione hydrolase (HAGH; glyoxalase II) is closely linked to the α-globin locus (HBα) on Chromosome 16. HAGH polymorphism in the mouse has now enabled the mapping of the murine homologue. Deletion mapping, congenic strain studies, and characterization of 41 recombinant inbred strains establish that the mouseHagh locus lies very close to the α-globin pseudogene (Hba-ps4) in the vicinity of the major histocompatibility locus (H-2) on chromosome 17. Several other loci have been identified previously that are also closely linked to the human α-globin locus but near the α-globin pseudogeneHba-ps4 in the mouse. These linkage relationships suggest that during the evolution of mice a translocation occurred that subdivided the α-globin locus, leaving one inactive α-globin gene still associated with theHagh locus and linked sequences, while moving and inserting the active α-globin locus and all distal sequences into an internal location on another autosome, the predecessor to mouse chromosome 11.
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    ADrosophila homologue of theSchizosaccharomyces pombe act2 gene (1993)
    Springer
    Biochemical genetics 31 (1993), S. 329-341 
    Details
    ISSN: 1573-4927
    Keywords: actin superfamily ; Drosophila genetics ; ATPase domain ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Diverse proteins that are 35% to 55% identical to actins have been discovered recently in yeasts, nematodes, and vertebrates. In order to study these proteins systematically and relate their functions to those of conventional actins, we are isolating the corresponding genes from the genetically tractable eukaryote,Drosophila melanogaster. Here we report the isolation and partial characterization of aDrosophila homologue of theSchizosaccharomyces pombe act2 gene. Degenerate oligonucleotide primers specifying peptides that are highly conserved within the actin protein superfamily were used in conjunction with polymerase chain reaction (PCR) to amplify a portion of theDrosophila gene that we have namedactr66B. The corresponding full-length cDNA sequence encodes a protein of 418 residues that is 65% identical to the product of theS. pombe act2 gene, 80% identical to the bovineact2 homologue, but only 48% identical to the principalDrosophila cytoplasmic actin encoded by theAct5C actin gene. Alignment of the yeast, bovine, andDrosophila actin-related proteins shows that they have four peptide insertions, relative to conventional actins, three of which are well placed to modify actin polymerization and one that is likely to perturb the binding of myosin. Locations of two of the fiveactr66B introns are conserved betweenDrosophila and yeast genes, further attesting that they evolved from a common ancestor and are likely to encode proteins having similar functions. We demonstrate that theDrosophila gene is located on the left arm of chromosome 3, within subdivision 66B. Finally, we show by RNA blot-hybridization that the gene is expressed at low levels, relative to conventional nonmuscle actin, in all developmental stages. From these and other observations we infer that the actr66B protein is a minor component of all cells, perhaps serving to modify the polymerization, structure, and dynamic behavior of actin filaments.
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    Genomic rearrangement of the α-globin gene complex during mammalian evolution (1993)
    Springer
    Biochemical genetics 31 (1993), S. 473-484 
    Details
    ISSN: 1573-4927
    Keywords: hydroxyacyl glutathione hydrolase (glyoxalase II) ; chromosome mapping ; evolution ; Mus musculus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In man, the gene for hydroxyacyl glutathione hydrolase (HAGH; glyoxalase II) is closely linked to the α-globin locus (HBα) on Chromosome 16. HAGH polymorphism in the mouse has now enabled the mapping of the murine homologue. Deletion mapping, congenic strain studies, and characterization of 41 recombinant inbred strains establish that the mouseHagh locus lies very close to the α-globin pseudogene (Hba-ps4) in the vicinity of the major histocompatibility locus (H-2) on chromosome 17. Several other loci have been identified previously that are also closely linked to the human α-globin locus but near the α-globin pseudogeneHba-ps4 in the mouse. These linkage relationships suggest that during the evolution of mice a translocation occurred that subdivided the α-globin locus, leaving one inactive α-globin gene still associated with theHagh locus and linked sequences, while moving and inserting the active α-globin locus and all distal sequences into an internal location on another autosome, the predecessor to mouse chromosome 11.
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    ADrosophila homologue of theSchizosaccharomyces pombe act2 gene (1993)
    Springer
    Biochemical genetics 31 (1993), S. 329-341 
    Details
    ISSN: 1573-4927
    Keywords: actin superfamily ; Drosophila genetics ; ATPase domain ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Diverse proteins that are 35% to 55% identical to actins have been discovered recently in yeasts, nematodes, and vertebrates. In order to study these proteins systematically and relate their functions to those of conventional actins, we are isolating the corresponding genes from the genetically tractable eukaryote,Drosophila melanogaster. Here we report the isolation and partial characterization of aDrosophila homologue of theSchizosaccharomyces pombe act2 gene. Degenerate oligonucleotide primers specifying peptides that are highly conserved within the actin protein superfamily were used in conjunction with polymerase chain reaction (PCR) to amplify a portion of theDrosophila gene that we have namedactr66B. The corresponding full-length cDNA sequence encodes a protein of 418 residues that is 65% identical to the product of theS. pombe act2 gene, 80% identical to the bovineact2 homologue, but only 48% identical to the principalDrosophila cytoplasmic actin encoded by theAct5C actin gene. Alignment of the yeast, bovine, andDrosophila actin-related proteins shows that they have four peptide insertions, relative to conventional actins, three of which are well placed to modify actin polymerization and one that is likely to perturb the binding of myosin. Locations of two of the fiveactr66B introns are conserved betweenDrosophila and yeast genes, further attesting that they evolved from a common ancestor and are likely to encode proteins having similar functions. We demonstrate that theDrosophila gene is located on the left arm of chromosome 3, within subdivision 66B. Finally, we show by RNA blot-hybridization that the gene is expressed at low levels, relative to conventional nonmuscle actin, in all developmental stages. From these and other observations we infer that the actr66B protein is a minor component of all cells, perhaps serving to modify the polymerization, structure, and dynamic behavior of actin filaments.
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    Models of the three-dimensional structures of echidna, horse, and pigeon lysozymes: Calcium-binding lysozymes and their relationship with α-lactalbumins (1994)
    Springer
    The protein journal 13 (1994), S. 569-584 
    Details
    ISSN: 1573-4943
    Keywords: Calcium-binding lysozyme ; α-lactalbumin ; three-dimensional structure ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Similarities in amino acid sequences, three-dimensional structures, and the exon-intron patterns of their genes have indicated thatc-type lysozymes andα-lactalbumins are homologous proteins, i.e., descended by divergent evolution from a common ancestor. Like theα-lactalbumins, echidna milk, horse milk, and pigeon eggwhite lysozymes all bind Ca(II). Models of their three-dimensional structures, based on their amino acid sequences and the known crystal structures of domestic hen eggwhite and human lysozymes and baboon and humanα-lactalbumins, have been built. The several structures have been compared and their relationships discussed.
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    URL: http://dx.doi.org/10.1007/BF01901539
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    Sequence of the iaa and ipt region of different Agrobacterium tumefaciens biotype III octopine strains: reconstruction of octopine Ti plasmid evolution (1991)
    Springer
    Plant molecular biology 16 (1991), S. 601-614 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; Ti plasmid ; evolution ; IS elements ; host range
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The TA regions of biotype III octopine/cucumopine (OC) Ti plasmids are closely related to the TL region of the biotype I octopine Ti plasmids pTiAch5 and pTi15955. Sequence analysis shows that the limited and wide host range biotype III OC TA regions are derived from a common ancestor structure which lacked the 6a gene found in the biotype I octopine TL region. The TA region of the wide host range OC Ti plasmids has conserved most of the original TL-like structure. In most wide host range OC isolates the TA-iaaH gene is inactivated by the insertion of an IS866 element. However, the TA region of the wide host range isolate Hm1 carries an intact TA-iaaH gene. This gene encodes a biologically active product, as shown by root induction tests and indole-3-acetic acid measurements. The limited host range OC Ti plasmids pTiAB3 and pTiAg57 have shorter TA regions which are derived from a wide host range TA region. The AB3 type arose by an IS868-mediated, internal TA region deletion which removed the iaa genes and part of the ipt gene and left a copy of IS868 at the position of the deleted fragment. The pTiAB3 iaa/ipt deletion was followed by insertion of a second IS element, IS869, immediately 3′ of the ipt gene. pTiAg57 underwent the same iaa-ipt deletion as pTiAB3, but lacks the IS868 and IS869 elements. Analysis of the various TA region structures provides a detailed insight into the evolution of the biotype III OC strains.
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    Characterization of members of a pseudogene subfamily of the wheat α-gliadin storage protein genes (1991)
    Springer
    Plant molecular biology 16 (1991), S. 335-337 
    Details
    ISSN: 1573-5028
    Keywords: Triticum ; wheat ; endosperm ; gliadin ; pseudogene ; duplication ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00020565
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    An equivalent to bacterialompR genes is encoded on the plastid genome of red algae (1992)
    Springer
    Plant molecular biology 18 (1992), S. 777-780 
    Details
    ISSN: 1573-5028
    Keywords: Cyanidium caldarium ; evolution ; Galdieria sulphuraria ; rRNA operon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00020019
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    Molecular cloning and evolutionary analysis of the calcium-modulated contractile protein, centrin, in green algae and land plants (1993)
    Springer
    Plant molecular biology 23 (1993), S. 1243-1254 
    Details
    ISSN: 1573-5028
    Keywords: calcium-binding protein ; centrin ; EF hand ; evolution ; green algae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Centrin (= caltractin) is a ubiquitous, cytoskeletal protein which is a member of the EF-hand superfamily of calcium-binding proteins. A centrin-coding cDNA was isolated and characterized from the prasinophyte green alga Scherffelia dubia. Centrin PCR amplification primers were used to isolate partial, homologous cDNA sequences from the green algae Tetraselmis striata and Spermatozopsis similis. Annealing analyses suggested that centrin is a single-copy-coding region in T. striata and S. similis and other green algae studied. Centrin-coding regions from S. dubia, S. similis and T. striata encode four colinear EF-hand domains which putatively bind calcium. Phylogenetic analyses, including homologous sequences from Chlamydomonas reinhardtii and the land plant Atriplex nummularia, demonstrate that the domains of centrins are congruent and arose from the two-fold duplication of an ancestral EF hand with Domains 1+3 and Domains 2+4 clustering. The domains of centrins are also congruent with those of calmodulins demonstrating that, like calmodulin, centrin is an ancient protein which arose within the ancestor of all eukaryotes via gene duplication. Phylogenetic relationships inferred from centrin-coding region comparisons mirror results of small subunit ribosomal RNA sequence analyses suggesting that centrin-coding regions are useful evolutionary markers within the green algae.
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    Structural features of the plastid ribosomal RNA operons of two red algae: Antithamnion sp. and Cyanidium caldarium (1991)
    Springer
    Plant molecular biology 16 (1991), S. 537-546 
    Details
    ISSN: 1573-5028
    Keywords: Antithamnion sp. ; chloroplast ; Cyanidium caldarium Geitler ; evolution ; red algae ; rRNA operon ; rRNA spacer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nucleotide sequences of the plastid 16S rDNA of the multicellular red alga Antithamnion sp. and the 16S rDNA/23S rDNA intergenic spacers of the plastid DNAs of the unicellular red alga Cyanidium caldarium and of Antithamnion sp. were determined. Sequence comparisons support the idea of a polyphyletic origin of the red algal and the higher-plant chloroplasts. Both spacer regions include the unsplit tRNAIle (GAU) and tRNAAla (UGC) genes and so the plastids of both algae form a homogeneous group with those of chromophytic algae and Cyanophora paradoxa characterized by ‘small-sized’ rDNA spacers in contrast to green algae and higher plants. Nevertheless, remarkable sequence differences within the rRNA and the tRNA genes give the plastids of Cyanidium caldarium a rather isolated position.
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    Evolution of the Rubisco operon from prokaryotes to algae: Structure and analysis of the rbcS gene of the brown alga Pylaiella littoralis (1991)
    Springer
    Plant molecular biology 17 (1991), S. 853-863 
    Details
    ISSN: 1573-5028
    Keywords: algae ; endosymbiosis ; evolution ; plastidial DNA ; Rubisco operon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rbcS gene coding for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) of the brown alga Pylaiella littoralis is located within the plastid genome and is transcribed as a single polycistronic mRNA with the gene for the large subunit of Rubisco, rbcL. The structure of the Rubisco operon from P. littoralis was determined. Molecular phylogenies for rbcS and rbcL with a wide range of prokaryotes and eukaryotes were constructed which are congruent with recent evidence for polyphyletic plastid origins. Both rbcL and rbcS of the β-purple bacterium Alcaligenes eutrophus clearly cluster with the rhodophyte and chromophyte proteins. The data suggest that the Rubisco operons of red algal and chromophytic plastids derive from β-purple eubacterial antecedents, rather than the cyanobacterial lineage of eubacteria from which other of their genes derive. This implies a lateral transfer of Rubisco genes from β-purple eubacterial ancestors to the cyanobacterial ancestor of rhodophyte and chromophyte plastids.
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    The atp1 and atp2 operons of the cyanobacterium Synechocystis sp. PCC 6803 (1991)
    Springer
    Plant molecular biology 17 (1991), S. 641-652 
    Details
    ISSN: 1573-5028
    Keywords: F-ATPase ; cyanobacteria ; operon ; evolution ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The two operons atp1 and atp2, encoding the subunits of the FOF1 ATP-synthase, have been cloned and sequenced from the cyanobacterium Synechocystis sp. PCC 6803. The organization of the different genes in the operons have been found to resemble that of the cyanobacteria Synechococcus sp. PCC 6301 and Anabaena sp. PCC 7120. The Synechocystis FOF1 ATP-synthase has nine subunits. A tenth open reading frame with unknown function was detected at the 5′ end of atp1, coding for a putative gene product similar to uncI in Escherichia coli. A promoter structure was inferred for the Synechocystis atp operons and compared to other known promoters of cyanobacteria. Even though the operon structure of atp1 and atp2 in Synechocystis resembles the corresponding operons of Synechococcus, the amino acid sequences of individual gene products show marked differences. Genetic distances between cyanobacterial genes and genes for ATP-synthase subunits from other species have been calculated and compiled into evolutionary trees.
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    URL: http://dx.doi.org/10.1007/BF00037050
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    Perspectives on zinc finger protein function and evolution - an update (1994)
    Springer
    Molecular biology reports 20 (1994), S. 1-8 
    Details
    ISSN: 1573-4978
    Keywords: review ; zinc finger protein ; DNA recognition ; evolution ; development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Complexity is one of the hallmarks that applies to C2H2 type zinc finger proteins (ZFPs). Structurally distinct clusters of zinc finger modules define an extremely large superfamily of nucleic acid binding proteins with several hundred, perhaps thousands of different members in vertebrates. Recent discoveries have provided new insights into the biochemistry of RNA and DNA recognition, into ZFP evolution and genomic organization, and also into basic aspects of their biological function. However, as much as we have learned, other fundamental questions about ZFP function remain highly enigmatic. This essay is meant to define what we personally feel are important questions, rather than trying to provide a comprehensive, encyclopaedic review.
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    URL: http://dx.doi.org/10.1007/BF00999848
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    The Rubisco activase (rca) gene is located downstream from rbcS in Anabaena sp. strain CA and is detected in other Anabaena/Nostoc strains (1993)
    Springer
    Plant molecular biology 21 (1993), S. 753-764 
    Details
    ISSN: 1573-5028
    Keywords: Rubisco activase ; rca ; rbcLrbcS ; cyanobacteria ; expression ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene encoding ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase (rca) was found downstream from the rbcLrbcS operon in the heterocystous cyanobacterium Anabaena sp. strain CA. Two unknown open reading frames were shown to be located between rbcS and rca in strain CA and all the genes, rbcLrbcS, ORF1, ORF2, and rca were in the same transcriptional orientation. The deduced amino acid sequence of the Anabaena Rubisco activase showed both similarities and differences to the plant enzyme with considerable differences at the carboxy and amino termini. Proposed ATP-binding sites were conserved in the cyanobacterial protein. Recombinant cyanobacterial Rubisco activase, however, reacted with antisera to spinach Rubisco activase. Hybridization studies, using the Anabaena sp. strain CA rca gene as a heterologous probe, detected homologous sequences in heterocystous Anabaena/Nostoc strains but not in unicellular or nonheterocystous filamentous cyanobacteria, suggestive of a close evolutionary relationship of chloroplasts and heterocystous cyanobacteria.
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    URL: http://dx.doi.org/10.1007/BF00027109
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    Chloroplast encoded thioredoxin genes in the red algae Porphyra yezoensis and Griffithsia pacifica: evolutionary implications (1994)
    Springer
    Plant molecular biology 25 (1994), S. 13-21 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; evolution ; red algae ; thioredoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A gene encoding a thioredoxin protein was identified in the chloroplast genome of the rhodophyte Porphyra yezoensis. The P. yezoensis trxA gene contains 324 bp and is transcribed into a 0.7 kb messenger RNA. Analysis of the transcription start site demonstrates that canonical chloroplast −10 and −35 sequences are not present. The deduced amino acid sequence of the thioredoxin gene from the red algae has the greatest similarity to type m thioredoxins, providing strong support for the hypothesis that type m thioredoxins in photosynthetic eukaryotes originated from an engulfed bacterial endosymbiont. Hybridization analysis of nuclear and chloroplast DNAs from several members of the phyla Chromophyta and Rhodophyta using P. yezoensis DNA as a probe demonstrated strong hybridization to the chloroplast and nuclear genomes of Griffithsia pacifica and a weak cross-hybridization to the chromophyte P. foliaceum. The G. pacifica chloroplast gene has a 66% identity with the P. yezoensis DNA, contains conserved active site amino acid residues, but lacks a methionine start codon.
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    URL: http://dx.doi.org/10.1007/BF00024194
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    Peptidergic motoneurons in the buccal ganglia of Aplysia californica Immunocytochemical, morphological, and physiological characterizations (1991)
    Springer
    Journal of comparative physiology 168 (1991), S. 323-336 
    Details
    ISSN: 1432-1351
    Keywords: Aplysia ; Motoneurons ; Immunocytochemistry ; Small cardioactive peptides ; Facilitation ; Depression ; Buccal ; Feeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We used physiological recordings, intracellular dye injections and immunocytochemistry to further identify and characterize neurons in the buccal ganglia of Aplysia calif ornica expressing Small Cardioactive Peptide-like immunoreactivity (SCP-LI). Neurons were identified based upon soma size and position, input from premotor cells B4 and B5, axonal projections, muscle innervation patterns, and neuromuscular synaptic properties. SCP-LI was observed in several large ventral neurons including B6, B7, B9, B10, and B11, groups of s1 and s2 cluster cells, at least one cell located at a branch point of buccal nerve n2, and the previously characterized neurons B1, B2 and B15. B6, B7, B9, B10 and B11 are motoneurons to intrinsic muscles of the buccal mass, each displaying a unique innervation pattern and neuromuscular plasticity. Combined, these motoneurons innervate all major intrinsic buccal muscles (I1/I3, I2, I4, I5, I6). Correspondingly, SCP-LI processes were observed on all of these muscles. Innervation of multiple nonhomologous buccal muscles by individual motoneurons having extremely plastic neuromuscular synapses, represents a unique form of neuromuscular organization which is prevalent in this system. Our results show numerous SCPergic buccal motoneurons with widespread ganglionic processes and buccal muscle innervation, and support extensive use of SCPs in the control of feeding musculature.
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    URL: http://dx.doi.org/10.1007/BF00198352
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    Occurrence and expression of acid phosphatase of Hymenoscyphus ericae (Read) Korf & Kernan, in isolation or associated with plant roots (1992)
    Springer
    Mycorrhiza 1 (1992), S. 137-146 
    Details
    ISSN: 1432-1890
    Keywords: Ericaceae ; Mycorrhizal fungi ; Acid phosphatase ; Protein expression ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The activity of acid phosphatase produced in pure culture by the endomycorrhizal fungus Hymenoscyphus ericae (Read) Korf & Kernan (H. ericae LPA 2) was inhibited by high phosphorus levels, alkaline pH, fluoride, molybdate and mannosidase, and activated by concanavalin A. Over 80% of the enzyme activity was due to two wall-bound acid phosphatase isozymes with the characteristics of mannose-rich glycoproteins. Antiserum was raised against the major, low-molecular-weight wall isozyme and its activity tested by immunoblotting and ELISA. The antiserum cross reacted 100% with exocellular (excreted) and 28% with cytoplasmic cellular fractions of H. ericae (LPA 2) cultures, and showed high reactivity with other strains of H. ericae but not with fungal isolates from Erica hispidula L. or E. mauritanica L. Ultrastructural localization of acid phosphatase by cytoenzymology and indirect immunogold labelling confirmed its association with the fungal wall in pure culture and showed that the influence of a high phosphorus level, fluoride and molybdate is through inactivation of the enzyme. Intense acid phosphatase activity, sensitive to the latter inhibitors, was also present on external hyphae growing over a host or non-host root but it was weak or absent from intracellular hyphae where these developed within a host root. Indirect immunolabelling confirmed that this acid phosphatase was of fungal origin and that the specific inhibitory effect of host cells is due to inactivation of the enzyme rather than repression of its synthesis. Possible implications of fungal acid phosphatase in ericoid endomycorrhizal infection processes are discussed together with mechanisms that may be regulating the enzyme activity.
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    URL: http://dx.doi.org/10.1007/BF00203287
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    Immunocytochemical study of seminal γ-glutamyltransferase using monoclonal antibodies (1994)
    Springer
    Cell & tissue research 277 (1994), S. 33-38 
    Details
    ISSN: 1432-0878
    Keywords: γ-Glutamyl transpeptidase ; Seminal γ-glutamyltransferase ; Prostate gland ; Seminal vesicle ; Monoclonal antibodies ; Immunocytochemistry ; Reproductive organs, male ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We produced three monoclonal antibodies, SG1, SG2 and SG3, specific for human seminal γ-glutamyltransferase when characterized by enzyme-linked immunosorbent assay and immunoblotting. Seminal γ-glutamyltransferase was localized, by immunostaining, to the epithelial cells of the ductus epididymidis, seminal vesicle and prostate gland with SG1, those of the prostate gland with SG2, and those of the seminal vesicle with SG3. Rabbit polyclonal anti-seminal γ-glutamyltransferase serum reacted with the proximal convolution of the kidney and the bile capillaries of the liver, and with the epithelial cells of the reproductive organs. However, immunoreactivity was not observed in the kidney or liver with the monoclonal antibodies. Thus, these monoclonal antibodies are probably all specific to seminal γ-glutamyltransferase but recognize different epitopes.
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    Localization of mammary-derived growth inhibitor in capillary endothelial cells of the bovine mammary gland (1994)
    Springer
    Cell & tissue research 277 (1994), S. 457-464 
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    ISSN: 1432-0878
    Keywords: Mammary-derived growth inhibitor ; Fatty acid-binding proteins ; Differentiation ; Vascularization ; Immunohistochemistry ; Immunocytochemistry ; Mammary gland ; Cow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mammary-derived growth inhibitor (MDGI) has previously been localized in the mammary parencyma, dependent on the stage of differentiation of the mammary gland. Here, we have elucidated the distribution of MDGI in the mammary stroma by a combined immunohisto-and cytochemical analysis with antibodies raised against MDGI. Distinct staining of capillary endothelial cells has been revealed. Although its subcellular distribution resembles former observations in secretory epithelial cells, the expression of MDGI in capillary endothelial cells clearly precedes that in secretory epithelial cells. On the other hand, no endothelial MDGI staining has been detected in bovine heart, which contains a fatty acid-binding protein almost identical to MDGI. The localization of MDGI in the mammary capillary endothelium is discussed in terms of its possible involvement in the intracellular transport of hydrophobic ligands or in the regulation of endothelial cell proliferation.
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    Luteinizing hormone —Releasing hormone (LH-RH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidase-antiperoxidase method (1974)
    Springer
    Cell & tissue research 153 (1974), S. 211-217 
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    ISSN: 1432-0878
    Keywords: Luteinizing hormone-releasing hormone ; Hypothalamus ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Utilizing the unlabeled antibody enzyme method, we report the distribution of hypothalamic elements immunoreactive with antibodies to luteinizing hormone-releasing hormone (LH-RH) in the rat. Immunostained elements, resembling neural processes, were distributed along a pathway corresponding to the tuberoinfundibular tract which appeared to terminate near vascular elements in the external layer of the preand post-infundibular median eminence. No cell bodies stained specifically for LH-RH. Similar topographic arrangements were noted (in coronal and sagittal sections) in diestrous females, ovariectomized females and a hypophysectomized male. The same results were obtained with three different preparations of antisera to LH-RH. Our studies agree with those of other investigators using immunohistochemical techniques as well as with localization studies of LH-RH in the hypothalamus using bioassay and radioimmunoassay. Our results suggest that the unlabeled antibody enzyme technique will have unique value for identifying and tracing fiber systems related to specific functions within the hypothalamus.
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    C-PON immunoreactive neurons in the neostriatum of the hedgehog (Erinaceus europaeus): a correlated light- and electron-microscopic study (1994)
    Springer
    Cell & tissue research 277 (1994), S. 177-181 
    Details
    ISSN: 1432-0878
    Keywords: Key words: C-PON ; Neuropeptide Y ; Neostriatum ; Immunocytochemistry ; Ultrastructure ; Erinaceus europaeus (Insectivora)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The present study provides light- and electron-microscopic immunocytochemical data on the presence of neurons that are immunoreactive to the C-terminal flanking peptide of neuropeptide Y, C-PON, in the neostriatum of the hedgehog (Erinaceus europaeus). Positive neurons have mostly fusiform or round perikarya from which two to four poorly branched processes arise. Immunostained fibers and puncta are also evenly distributed throughout the neostriatum. Ultrastructurally, each neuron exhibits a deeply invaginated nucleus surrounded by abundant cytoplasm with a well-developed rough endoplasmic reticulum and Golgi apparatus. Positive neurons receive symmetric and asymmetric synapses from unlabeled terminals. The results of this study can be correlated with previous findings, as the C-PON-positive neurons of the hedgehog resemble medium-sized neostriatal neurons that are known to be local circuit neurons exhibiting C-PON in the rat. Thus, a high degree of C-PON neuronal system phylogenetic conservation and function can be postulated for the neostriatum of mammals.
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    Ontogeny of some endocrine cells of the digestive tract in sea bass (Dicentrarchus labrax): An immunocytochemical study (1994)
    Springer
    Cell & tissue research 277 (1994), S. 373-383 
    Details
    ISSN: 1432-0878
    Keywords: Endocrine cells ; Gut ; Ontogeny ; Regulatory peptides ; Immunocytochemistry ; Dicentrarchus labrax (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Serotonin- and ten peptide-immunoreactive (IR) cell types were identified in the digestive tract of sea bass (Dicentrarchus labrax L.) larvae of four morphofunctional phases ranging in age from hatching to 61 days. The sequence of appearance and location of endocrine cells during ontogenetic development of the larvae was determined. The differentiation of endocrine cells followed a distal-proximal gradient in the gut which paralleled the morphofunctional differentiation. Serotonin-IR cells were identified in the last portion of the digestive tract from phase I onwards and in the gastric region from phase III, before these regions were morphofunctionally differentiated; met-enkephalin-IR cells were identified from phase II onwards in both the differentiated rectum and the undifferentiated intestine; cholecystokinin (CCK)- and synthetic human gastrin-34-IR cells were located only in the intestine and first found in the undifferentiated intestine of phase II; human gastrin-17-, peptide YY (PYY)- and neuropeptide Y (NPY)-IR cells appeared in the intestine from phase II and in stomach in phase IV, when it showed gastric glands; pancreatic polypeptide (PP)- and glucagon-IR cells were observed in both intestine and stomach, but insulin- and somatostatin-IR cells only in stomach, from phase III, during which the intestine but not the stomach was differentiated. PP- and PYY-, PP- and glucagon-, and PYY- and glucagon-like immunoreactivities coexisted from their first appearance in some cells of the gut.
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    Ontogeny of some endocrine cells of the digestive tract in sea bass (Dicentrarchus labrax): An immunocytochemical study (1994)
    Springer
    Cell & tissue research 277 (1994), S. 373-383 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Endocrine cells ; Gut ; Ontogeny ; Regulatory peptides ; Immunocytochemistry ; Dicentrarchuslabrax (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Serotonin- and ten peptide-immunoreactive (IR) cell types were identified in the digestive tract of sea bass (Dicentrarchus labrax L.) larvae of four morphofunctional phases ranging in age from hatching to 61 days. The sequence of appearance and location of endocrine cells during ontogenetic development of the larvae was determined. The differentiation of endocrine cells followed a distal-proximal gradient in the gut which paralleled the morphofunctional differentiation. Serotonin-IR cells were identified in the last portion of the digestive tract from phase I onwards and in the gastric region from phase III, before these regions were morphofunctionally differentiated; met-enkephalin-IR cells were identified from phase II onwards in both the differentiated rectum and the undifferentiated intestine; cholecystokinin (CCK)- and synthetic human gastrin-34-IR cells were located only in the intestine and first found in the undifferentiated intestine of phase II; human gastrin-17-, peptide YY (PYY)- and neuropeptide Y (NPY)-IR cells appeared in the intestine from phase II and in stomach in phase IV, when it showed gastric glands; pancreatic polypeptide (PP)- and glucagon-IR cells were observed in both intestine and stomach, but insulin- and somatostatin-IR cells only in stomach, from phase III, during which the intestine but not the stomach was differentiated. PP- and PYY-, PP- and glucagon-, and PYY- and glucagon-like immunoreactivities coexisted from their first appearance in some cells of the gut.
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    Chemically defined collateral projections from the pons to the central nucleus of the amygdala and hypothalamic paraventricular nucleus in the rat (1994)
    Springer
    Cell & tissue research 277 (1994), S. 289-295 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Serotonin ; Substance P ; Choline-acetyltransferase ; Retrograde tracers ; Immunocytochemistry ; Laterodorsal tegmental nucleus ; Dorsal raphe nucleus ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Triple fluorescence labelling was employed to reveal the distribution of chemically identified neurons within the pontine laterodorsal tegmental nucleus and dorsal raphe nucleus which supply branching collateral input to the central nucleus of the amygdala and hypothalamic paraventricular nucleus. The chemical identity of neurons in the laterodorsal tegmental nucleus was revealed by immunocytochemical detection of choline- acetyltransferase or substance P; in the dorsal raphe nucleus, the chemical content of the neurons was revealed with antibody recognizing serotonin. The projections were defined by injections of two retrograde tracers, rhodamine- and fluorescein-labelled latex microspheres, in the central nucleus of the amygdala and paraventricular nucleus, respectively. Neurons projecting to both the central nucleus of the amygdala and the paraventricular nucleus were distributed primarily within the caudal extensions of the laterodorsal tegmental nucleus and dorsal raphe nucleus. Approximately 11% and 7% of the labelled cells in the laterodorsal tegmental nucleus and dorsal raphe nucleus projected via branching collaterals to the paraventricular nucleus and central nucleus of the amygdala. About half of these neurons in the laterodorsal tegmental nucleus were cholinergic, and one-third were substance-P-ergic; in the dorsal raphe nucleus, approximately half of the neurons containing both retrograde tracers were serotonergic. These results indicate that pontine neurons may simultaneously transmit signals to the central nucleus of the amygdala and paraventricular nucleus and that several different neuroactive substances are found in the neurons participating in these pathways. This coordinated signalling may lead to synchronized responses of the central nucleus of the amygdala and paraventricular nucleus for the maintenance of homeostasis. Interactions between different neuroactive substances at the target site may serve to modulate the responses of individual neurons.
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    URL: http://dx.doi.org/10.1007/s004410050155
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    Ultrastructural, cytochemical, and immunocytochemical characterization of haemocytes of the hard tick Ixodes ricinus (Acari; Chelicerata) (1994)
    Springer
    Cell & tissue research 277 (1994), S. 493-504 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Haemocytes ; Immunocytes ; invertebrate ; Immunity ; Immunocytochemistry ; Ixodes ricinus (Chelicerata)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Haemocytes of the hard tick Ixodes ricinus were characterized on the basis of their ultrastructure, their ability to ingest foreign material, and to produce or store molecules of the immune defence. Distinction was made between types of haemocytes according to the absence or presence of granular inclusions, shape and size of the lysosomal compartment or the rough endoplasmic reticulum, and ultrastructural and functional similarity to the corresponding haemocytes of insects. Three types of haemocytes were found in adult ticks: plasmatocytes and type-I and type-II granular haemocytes, respectively. The precipitated reaction product of acid phosphatase activity revealed the shape of the lysosomal compartment. The additional injection of particulate materials into the haemocoel further revealed the endocytic activity of the haemocytes. The lysozyme-like immunoreactivity of the haemocytes suggests bactericidal potential. Detection of immunoreactivity in haemocytes to a 25 kDa antigenic protein involved in cuticle formation further suggests their involvement in wound healing and encapsulation.
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    Development of sensory innervation in chick skin: comparison of nerve fibre and chondroitin sulphate distributions in vivo and in vitro (1994)
    Springer
    Cell & tissue research 277 (1994), S. 519-529 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Skin ; Development ; ontogenetic ; Chondroitin sulphate proteoglycan ; Neuritic guidance ; Immunocytochemistry ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In bird skin, nerve fibres develop in the dermis but do not enter the epidermis. In co-cultures of 7-day-old chick embryo dorsal root ganglia and epidermis, the neurites also avoid the epidermis. Previous studies have shown that chondroitin sulphate proteoglycans may be involved. Chondroitin sulphate has therefore been visualized by immunocytochemistry, using the monoclonal antibody CS-56, both in vivo and in vitro using light and electron microscopy. Its distribution was compared to those of 2 other chondroitin sulphate epitopes and to that of the growing nerve fibres. In cultures of epidermis from 7-day-old embryonic chicks, immunoreactivity is found uniformly around the epidermal cells while at 7.5 days the distribution in dermis is heterogeneous, and particularly marked in feather buds. In vivo, chondroitin sulphate immunoreactivity is detected in the epidermis, on the basal lamina, on the surfaces of fibroblasts and along collagen fibrils. This localization is complementary to the distribution of cutaneous nerves. Chondroitin sulphate in the basal lamina could prevent innervation of the epidermis and the dermal heterogeneities could partly explain the nerve fibres surrounding the base of the feathers. Chondroitin sulphate could therefore be important for neural guidance in developing chick skin.
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    Immunoreactivity of the corpuscles of Stannius of the garpike, Lepisosteus osseus, to antisera against salmon and trout stanniocalcins (1994)
    Springer
    Cell & tissue research 277 (1994), S. 511-518 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Corpuscles of Stannius ; Stanniocalcin ; Immunocytochemistry ; Immunohistochemistry ; Western blot ; Lepisosteus osseus (Holostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Stanniocalcin-immunoreactive cells were localized in the corpuscles of Stannius of a holostean fish, the garpike (Lepisosteus osseus), using antisera against salmon and trout stanniocalcins and the peroxidase-antiperoxidase and protein A-gold immunohistochemical methods. The stanniocalcin-immunoreactive cells were periodic acid-Schiff-positive, and antibody staining was abolished if the antiserum was preabsorbed with corpuscle homogenate. Immunocytochemistry revealed two reactive cell types in the glandular parenchyma, and immunoreactivity was confined to the secretory granules. Staining of the granules was also abolished when the antisera were blocked with crude corpuscle homogenate. When corpuscle extracts from garpike were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot analysis, a single dense band was evident with a molecular weight of ∼68 kDa under non-reducing conditions, whereas three bands were observed (∼29, ∼31, and ∼34 kDa) under reducing conditions. Staining of all bands disappeared following preabsorption of the antiserum with salmon stanniocalcin, trout stanniocalcin, or garpike corpuscle extract. The results are compared with stanniocalcins from another extant holostean, the bowfin (Amia calva), and from more modern bony fishes, the teleosts.
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    Immunoreactivity of the corpuscles of Stannius of the garpike, Lepisosteus osseus, to antisera against salmon and trout stanniocalcins (1994)
    Springer
    Cell & tissue research 277 (1994), S. 511-518 
    Details
    ISSN: 1432-0878
    Keywords: Corpuscles of Stannius ; Stanniocalcin ; Immunocytochemistry ; Immunohistochemistry ; Western blot ; Lepisosteus osseus (Holostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Stanniocalcin-immunoreactive cells were localized in the corpuscles of Stannius of a holostean fish, the garpike (Lepisosteus osseus), using antisera against salmon and trout stanniocalcins and the peroxidase-antiperoxidase and protein A-gold immunohistochemical methods. The stanniocalcin-immunoreactive cells were periodic acid-Schiff-positive, and antibody staining was abolished if the antiserum was preabsorbed with corpuscle homogenate. Immunocytochemistry revealed two reactive cell types in the glandular parenchyma, and immunoreactivity was confined to the secretory granules. Staining of the granules was also abolished when the antisera were blocked with crude corpuscle homogenate. When corpuscle extracts from garpike were subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot analysis, a single dense band was evident with a molecular weight of ∼68 kDa under non-reducing conditions, whereas three bands were observed (∼29, ∼31, and ∼34 kDa) under reducing conditions. Staining of all bands disappeared following preabsorption of the antiserum with salmon stanniocalcin, trout stanniocalcin, or garpike corpuscle extract. The results are compared with stanniocalcins from another extant holostean, the bowfin (Amia calva), and from more modern bony fishes, the teleosts.
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    Keratin expression: a measure of phenotypic modulation of human prostatic epithelial cells by growth inhibitory factors (1994)
    Springer
    Cell & tissue research 277 (1994), S. 11-18 
    Details
    ISSN: 1432-0878
    Keywords: Prostate gland ; Keratin ; Vitamin A ; Epithelium ; Immunocytochemistry ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Expression of certain cytokeratins can be indicative of the state of differentiation of epithelial cells. The basal cells in the normal adult human prostatic epithelium are characterized by the expression of cytokeratins 5 and 14, whereas the secretory luminal cells contain cytokeratins 8 and 18. Cells cultured from the prostatic epithelium expressed cytokeratins 5, 8, and 18, and thus had features of both basal and luminal cells. Certain growth-inhibitory conditions altered keratin expression in conjunction with growth modulation. Deletion of peptide factors and hormones from the culture medium induced the expression of cytokeratins 1 and 10, associated with a squamous phenotype. These same squamous keratins were found in very dense, stratified cultures that were maintained at confluency in standard, complete medium for extended periods. Retinoic acid enhanced the expression of secretory luminal cell-associated cytokeratins 8 and 18 in semi-confluent cultures. Other growth inhibitory factors such as suramin, transforming growth factor-β, and interferon-γ had no effect on keratin expression. These observations indicate that the differentiation of prostatic epithelial cells can be directed toward alternate pathways, either squamous or secretory, by different growth-inhibitory conditions. However, not all growth inhibitory factors altered differentiation, demonstrating that growth inhibition in itself is not a sufficient inducer of differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303075
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  • 93
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    Intra-acrosomal organization of a 90-kilodalton antigen during spermiogenesis in the rat (1994)
    Springer
    Cell & tissue research 277 (1994), S. 61-67 
    Details
    ISSN: 1432-0878
    Keywords: Acrosome development ; Antigen localization ; Intra-acrosomal migration ; Golgi apparatus ; Spermiogenesis ; Immunocytochemistry ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The localization of an acrosomal protein was studied using a monoclonal antibody MN7 raised against mouse spermatozoa. MN7 specifically recognized the anterior acrosome of several mammalian (mouse, rat, hamster) spermatozoa fixed with paraformaldehyde. An immunoblot study with periodate treatment showed that MN7 recognized a carbohydrate region of a 90 kDa protein in an extract of mouse and rat cauda epididymal spermatozoa. The change in distribution of the MN7 antigen during acrosome development was investigated in the rat testis using the pre-embedding immunoperoxidase technique. The antigen first appeared in the proacrosomic granules of spermatids in steps 1–2. Small vesicles adjacent to the outer acrosomal membrane and the developing acrosomic system were immunoreactive during steps 4–7. The majority of the antigen was then redistributed to the head-cap portion during steps 8–18, and finally restricted to the anterior acrosome in the step 19-spermatid. These results suggest that the antigen is transported to the acrosome by way of the vesicles that originate from the Golgi apparatus during early spermiogenesis, and are then delivered to the final destination within the acrosome by the intra-acrosomal migration during late spermiogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303081
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  • 94
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    Functional morphology of the neuropeptidergic light-yellow-cell system in pulmonate snails (1994)
    Springer
    Cell & tissue research 277 (1994), S. 531-538 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Light yellow neuropeptidergic cells ; Immunocytochemistry ; Blood pressure regulation ; Pulmonata ; Lymnaea stagnalis ; Helix aspersa (Mollusca)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The light yellow neuropeptidergic cell system of the basommatophoran snail Lymnaea stagnalis is homologous to the R3-R14 system of the opisthobranch Aplysia californica, and produces three different neuropeptides. Systems homologous to the light yellow cells of Lymnaea stagnalis have been investigated morphologically in two Basommatophora (Lymnaea ovata, Bulinus truncatus) and three Stylommatophora (Helix aspersa, Cepaea nemoralis, Deroceras reticulatum). To this end, an antibody to synthetic light-yellow-cell peptide-II and oligonucleotides to mRNAs encoding parts of peptide-I and peptide-III, were used. The in situ hybridization probes gave negative results. On the other hand, neuronal cell clusters were observed in the central nervous system of all species studied by immunocytochemistry. These clusters were located in the ganglia of the visceral complex. The neurons project axons into all nerves of these ganglia, especially into the pallial nerves, into the connective tissue of the central nervous system, and into the neuropile of various ganglia. The morphology of the systems is similar to that of the light-yellow-cell system of Lymnaea stagnalis. In all species, the wall of the aorta was innervated by immunoreactive axons. Peripheral innervation by the light-yellow-cell system was investigated in Helix aspersa and Deroceras reticulatum. Serial and alternate sections of whole snails were studied. Reconstructions were made of the heart-kidney-lung complex of these animals. In both species, the muscular vessels of the pulmonary system at the right side of the body were strongly innervated by immunoreactive axons. Furthermore, immunopositive innervation was observed to muscles in the secondary ureter-pneumostome area. The light-yellow-cell system of pulmonates is thus probably involved in the regulation of blood pressure and urine release.
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    URL: http://dx.doi.org/10.1007/BF00300226
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  • 95
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    The infundibular organ of the lancelet (Branchiostoma lanceolatum, Acrania): an immunocytochemical study (1994)
    Springer
    Cell & tissue research 277 (1994), S. 107-114 
    Details
    ISSN: 1432-0878
    Keywords: Reissner's fiber ; Infundibular organ ; Immunocytochemistry ; Lectin binding ; Flexural organ ; Amphioxus, Branchiostoma lanceolatum (Acrania)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Reissner's fibers are secretions produced by different ependymal areas of the chordate brain, viz., in adult vertebrates, by the dorsal subcommissural organ, and in all stages of cephalochordates (Branchiostoma lancelets), by the ventral infundibular organ. Fibers produced by these different organs are seemingly identical and the two fiber sources also share some immunocytochemical and lectin-binding properties. The secretions in these two glands are, however, not identical; the infundibular organ cells are strongly reactive with antibodies against vertebrate Reissner's fibers, but they do not react with antibodies raised against the source of the vertebrate fibers, viz., the subcommissural organ. The results support the possibility that, in adult vertebrates, the Reissner's fibers are composed of material not only from the subcommissural organ, but also from another, not yet identified, source that is identical or equivalent to the infundibular organ of the lancelet. There are indications that the infundibular organ is immunocytochemically closely akin to some secretory cells in the vertebrate embryonic brain and also to those that produce the juvenile vertebrate Reissner's fibers, viz., secretory cells in the flexural organ.
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    URL: http://dx.doi.org/10.1007/BF00303086
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  • 96
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    Chemically defined collateral projections from the pons to the central nucleus of the amygdala and hypothalamic paraventricular nucleus in the rat (1994)
    Springer
    Cell & tissue research 277 (1994), S. 289-295 
    Details
    ISSN: 1432-0878
    Keywords: Serotonin ; Substance P ; Choline-acetyltransferase ; Retrograde tracers ; Immunocytochemistry ; Laterodorsal tegmental nucleus ; Dorsal raphe nucleus ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Triple fluorescence labelling was employed to reveal the distribution of chemically identified neurons within the pontine laterodorsal tegmental nucleus and dorsal raphe nucleus which supply branching collateral input to the central nucleus of the amygdala and hypothalamic paraventricular nucleus. The chemical identity of neurons in the laterodorsal tegmental nucleus was revealed by immunocytochemical detection of choline-acetyltransferase or substance P; in the dorsal raphe nucleus, the chemical content of the neurons was revealed with antibody recognizing serotonin. The projections were defined by injections of two retrograde tracers, rhodamine-and fluorescein-labelled latex microspheres, in the central nucleus of the amygdala and paraventricular nucleus, respectively. Neurons projecting to both the central nucleus of the amygdala and the paraventricular nucleus were distributed primarily within the caudal extensions of the laterodorsal tegmental nucleus and dorsal raphe nucleus. Approximately 11% and 7% of the labelled cells in the laterodorsal tegmental nucleus and dorsal raphe nucleus projected via branching collaterals to the paraventricular nucleus and central nucleus of the amygdala. About half of these neurons in the laterodorsal tegmental nucleus were cholinergic, and one-third were substance-P-ergic; in the dorsal raphe nucleus, approximately half of the neurons containing both retrograde tracers were serotonergic. These results indicate that pontine neurons may simultaneously transmit signals to the central nucleus of the amygdala and paraventricular nucleus and that several different neuroactive substances are found in the neurons participating in these pathways. This coordinated signalling may lead to synchronized responses of the central nucleus of the amygdala and paraventricular nucleus for the maintenance of homeostasis. Interactions between different neuroactive substances at the target site may serve to modulate the responses of individual neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327776
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  • 97
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    Distribution and anatomy of GABA-like immunoreactive neurons in the central and peripheral nervous system of the snail Helix pomatia (1994)
    Springer
    Cell & tissue research 277 (1994), S. 189-198 
    Details
    ISSN: 1432-0878
    Keywords: Key words: GABA ; Glutamate ; Immunocytochemistry ; Nervous system ; central ; Nervous system ; peripheral ; Helix pomatia (Mollusca)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Gamma-aminobutyric acid (GABA)-like immunoreactive neurons were studied in the central and peripheral nervous system of Helix pomatia by applying immunocytochemistry on whole-mount preparations and serial paraffin sections. GABA-immunoreactive cell bodies were found in the buccal, cerebral and pedal ganglia, but only GABA-immunoreactive fibers were found in the viscero-parietal-pleural ganglion complex. The majority of GABA-immunoreactive cell bodies were located in the pedal ganglia but a few could be found in the buccal ganglia. Varicose GABA-ir fibers could be seen in the neuropil areas and in distinct areas of the cell body layer of the ganglia. The majority of GABA-ir axonal processes run into the connectives and commissures of the ganglia, indicating an important central integrative role of GABA-immunoreactive neurons. GABA may also have a peripheral role, since GABA-immunoreactive fibers could be demonstrated in peripheral nerves and the lips. Glutamate injection did not change the number or distribution of GABA-immunoreactive neurons, but induced GABA immunoreactivity in elements of the connective tissue ensheathing the muscle cells and fibers of the buccal musculature. This shows that GABA may be present in different non-neural tissues as a product of general metabolic pathways.
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    URL: http://dx.doi.org/10.1007/BF00303096
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  • 98
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    Immunologicalization of complement C1s and matrix metalloproteinase 9 (92kDa gelatinase/type IV collagenase) in the primary ossification center of the human femur (1994)
    Springer
    Cell & tissue research 277 (1994), S. 239-245 
    Details
    ISSN: 1432-0878
    Keywords: Bone ; Ossification ; Cartilage ; Matrix ; Chondrocytes ; Complement ; Matrix metalloproteinase ; Immunocytochemistry ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The first component of complement $$C\bar 1s$$ has been shown to degrade type I and type II collagens (Yamaguchi et al. 1990), the latter of which is a major constituent of the cartilage matrix. In order to understand the physiological roles of $$C\bar 1s$$ in cartilage resorption, the expression of C1s was examined by immunohistochemistry in the primary ossification center where the matrix is removed and replaced by bone marrow. Hypertrophic chondrocytes, endothelium and hematogenous elements in the capillary buds were intensely stained by a monoclonal antibody against C1s. Matrix metalloproteinase 9 (MMP-9, 92kDa gelatinase/type IV collagenase) was also immunolocalized in hypertrophic chondrocytes, mesenchymal cells in the primitive bone marrow and the cartilage matrix adjacent to the marrow. In addition, $$C\bar 1s$$ was found to activate the zymogen of MMP-9. These observations suggest that $$C\bar 1s$$ and MMP-9 coordinately participate in matrix degradation in cartilage.
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    URL: http://dx.doi.org/10.1007/BF00327771
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  • 99
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    Basement membrane components of bronchial epithelium in humans suffering from chronic nonspecific lung diseases (1994)
    Springer
    Cell & tissue research 277 (1994), S. 505-510 
    Details
    ISSN: 1432-0878
    Keywords: Collagen IV ; Laminin ; Immunocytochemistry ; Basement membrane ; Bronchial epithelium ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Collagen IV and laminin are important constituents of the basement membrane (BM). By use of immunocytochemistry we examined the occurrence and distribution of these two components in the BM beneath normal, mucoid and metaplastic epithelium of large bronchi in 22 adults suffering from chronic nonspecific lung diseases. Both collagen IV and laminin were expressed as a thin and continuous layer beneath the epithelium in most tissue specimens with normal epithelium. In a few specimens the layer showed interruptions with a patchy distribution of the immunoreactivity. Three patterns of distribution of BM components were found under the metaplastic epithelium. Total absence of immunoreactive collagen IV and laminin was the most common variant. Weak and scarce staining for both proteins in the BM characterized the second pattern. The third variant showed strong collagen IV immunoreactivity but lack of laminin. The BM beneath the mucoid epithelium was characterized by irregular distribution of collagen IV and laminin. We suggest that the occurrence and distributional pattern of the BM components are related to the type of overlying epithelium and connected with an altered synthesis of these components.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00300223
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  • 100
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    Localisation par immunocytologie de sécrétions apparentées aux hormones thyréotropes, gonadotropes et corticotropes dans l'hypophyse distale de l'Axolotl (Ambystoma mexicanum, Shaw) (1973)
    Springer
    Cell & tissue research 142 (1973), S. 539-548 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Axolotl ; Glycoprotein hormones ; Corticotrophs ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les anticorps anti-TSH (hormone thyréotrope) d'origine bovine, anti-LH β (hormone lutéinisante) d'origine ovine et anti β (1–24) corticotropine de synthèse ont été appliqués à l'hypophyse de l'Axolotl (Ambystoma mexicanum, Shaw) selon une technique d'immunofluorescence histologique indirecte. Les anticorps anti-TSH se fixent sur une population de cellules fusiformes localisées dans les régions caudale et ventrale de l'hypophyse distale. Les éléments révélés par les anticorps anti-LH β possèdent un noyau ovalaire entouré d'un cytoplasme intensément fluorescent et sont répartis dans l'ensemble du lobe hypophysaire distal. Les cellules fixant l'anticorps anti β (1–24) corticotropine sont disposées en bordure de l'éminence médiane et présentent un noyau ovalaire et un cytoplasme abondant. Il s'agirait respectivement des cellules à fonction thyréotrope, gonadotrope et corticotrope. L'examen des caractéristiques cytologiques et histochimiques de ces trois types cellulaires complète ces données immunologiques.
    Notes: Summary Antisera prepared respectively against TSH (thyreotropin) of bovine origin, against LH (luteotropin) of ovine origin and against synthetic β (1–24) corticotropin were applied to histological sections of Axolotl pituitary (Ambystoma mexicanum, Shaw) with the indirect immunofluorescence procedure. The anti-TSH antibodies from the first antiserum attach themselves to a population of fusiform cells situated in the caudal and ventral areas of the pars distalis. The cells revealed by the second antiserum show an oval nucleus surrounded by a strongly fluorescent cytoplasm. They are scattered throughout the whole pars distalis. The cells revealed by the third antiserum are arranged around the median eminence. Their nucleus is oval and their cytoplasm abundant. Presumably, these three cell types correspond respectively to the thyreotropic, gonadotropic and corticotropic cells as indicated by a parallel examination of their cytological and histochemical characteristics.
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    URL: http://dx.doi.org/10.1007/BF00306714
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