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  • Articles  (170)
  • resistance  (170)
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  • 1975-1979  (17)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (170)
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  • Articles  (170)
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  • Springer  (170)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Integrated pest management reviews 1 (1995), S. 65-69 
    ISSN: 1572-9745
    Keywords: Glycine max ; Heterodera glycines ; inheritance ; resistance ; soybean ; soybean cyst nematode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetics of resistance to soybean cyst nematode (SCN), Heterodera glycines Ichinohe is very complex. Crosses involving PI 437654, which is resistant to all races of cyst nematodes with other sources of resistance (Peking, PI 88788, and PI 90763) indicated that resistance to race 3 was controlled by four genes, two of which were dominant resistance genes and the other two were recessive resistance genes. For race 5, a four gene model with two recessive and two dominant resistance genes in epistasis has been proposed. For race 14, the results suggested a three gene model with one dominant and two recessive alleles. Several other plant introductions have been isolated which have different genes conditioning resistance. Most of the currently grown soybean varieties derived resistance from Peking and/or PI 88788. Resistance to SCN in these soybean varieties has broken down because of the emergence of several new races and populations of SCN. The use of PI 437654 or Hartwig and other plant introductions with different genes for resistance will broaden genetic diversity and stabilize yield.
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  • 2
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    Integrated pest management reviews 3 (1998), S. 209-224 
    ISSN: 1572-9745
    Keywords: cassava ; Manihot esculenta ; Africa ; cassava mosaic virus disease ; whitefly vector (Bemisia tabaci) ; control ; varietal diversity ; resistance ; deployment ; durability ; breakdown ; phytosanitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cassava mosaic virus disease (CMD) is prevalent and causes serious losses in cassava (Manihot esculenta) in southern India and in many parts of sub-Saharan Africa. The disease is caused by viruses of the Geminiviridae that are transmitted by the whitefly Bemisia tabaci and disseminated in the stem cuttings used routinely for propagation. The main approach to control is through the use of virus-resistant varieties, but suitable ones are not always available and susceptible varieties are still widely grown. This explains why CMD continues to be a problem in many areas. CMD-resistant varieties have several features which are considered in this review:- • They are not readily infected, even when exposed to large amounts of vector-borne inoculum. • When infected they develop symptoms that tend to be inconspicuous and not associated with obvious deleterious effects on growth or yield. Moreover, the symptoms become even less conspicuous as growth proceeds and plants may eventually recover and become symptomless. • Infected plants support a low virus content and they are likely to be a poor source of inoculum from which further spread can occur. • Virus is not fully systemic within infected plants which can be a source of uninfected planting material when stem cuttings are collected for further propagation. This phenomenon is referred to as ‘reversion’ and it has an important ‘cleansing effect’ in restricting or preventing the progressive deterioration in health status that would otherwise occur during successive cycles of vegetative propagation. The available information on the different components of resistance is discussed and it is concluded that they are inter-related features of the same basic mechanism that restricts virus entry, replication and movement within the host. It is argued that the effectiveness and durability of virus-resistant varieties are likely to be influenced by the way in which they are deployed. However, this topic has received little attention from researchers and there is continuing uncertainty on the effects of CMD on the yield of resistant varieties and on the role of phytosanitation. This involves the use of virus-free planting material and the removal (roguing) of any additional diseased plants that occur. Some consider that these procedures complement the use of virus-resistant varieties and should be adopted, whereas others argue that they are unnecessary and inappropriate. It is concluded that there is considerable scope for utilizing resistant varieties more widely and more effectively than at present, but in doing so it is important to avoid eroding the genetic diversity that is currently such a marked feature of cassava cultivation in Africa.
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  • 3
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    Potato research 32 (1989), S. 397-404 
    ISSN: 1871-4528
    Keywords: resistance ; inoculation of tuber slices ; infection of plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eye-bearing slices, cut from healthy potato tubers and placed between Parafilm membranes, were inoculated with potato leafroll virus (PLRV) byMyzus persicae. PLRV was detected by ELISA and by transmission tests in tuber slices and in plants grown from the slices of the susceptible cv. Désirée, but not in those of the resistant cv. Arkula. These results suggest that PLRV replication and transport within tuber phloem is controlled by specific mechanisms of resistance.M. persicae was also able to acquire and transmit PLRV toPl floridana from slices cut from tubers of infected plants. The aphids effectively transmitted PLRV from slices cut from the sprouting rose end but they failed to transmit it from slices cut from the heel end of tubers.
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  • 4
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    Potato research 41 (1998), S. 143-153 
    ISSN: 1871-4528
    Keywords: resistance ; Solanum tuberosum L. cultivars ; glycoalkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of freeze dried potato powder derived from tubers (cvs Désirée and Epicure) exposed to light as a culture medium on the growth, number and spore size of nine fungal pathogens was determined in vitro with particular emphasis on the role of glycoalkaloids. Greater reductions in pathogen growth reflected increased exposure to light of tubers prior to freeze drying. High correlations (% R2 adj) were obtained between glycoalkaloid concentration and growth rate of the majority of pathogens tested. Where spores were produced, numbers were in general significantly less when cultured on freeze dried powder derived from tubers exposed to light. No effects on spore size ofFusarium species were recorded, but spore length of remaining pathogens was reduced and spore width increased, with the exception ofC. coccodes where spore length and width was increased following culture onto freeze dried powder derived from tubers exposed to light.
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  • 5
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    Potato research 38 (1995), S. 199-210 
    ISSN: 1871-4528
    Keywords: PVY ; resistance ; progeny tests ; inheritance ; potato breeding ; Solanum tuberosum L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Segregation for extreme resistance to PVY was evaluated in progenies derived from crossing two extremely resistant potato clones with parents differing in resistance. Resistance was evaluated after mechanical inoculation with PVYO and PVYN, and after graft inoculation with PVYO. Biological and serological tests (ELISA) were used for virus detection. The extreme resistance is governed by a single dominant gene, but observed segregations deviated from the expected ratios. Considerable modifying effects were detectable, depending on the potato genotype and virus isolate, for a significant excess of susceptible genotypes was observed in some progenies. Moreover, genotypes with non-parental types of resistance to PVY were observed.
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  • 6
    ISSN: 1871-4528
    Keywords: bacterial wilt ; resistance ; transformation ; Agrobacterium ; S. tuberosum L. ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transgenic potato plants of cv. Désirée carrying an antibacterial gene, coding for a cecropin lytic peptide analogue, were inoculated with a virulent strain ofPseudomonas solanacearum under controlled conditions. The disease index scored during three repeated infection trials indicated an increased variability in plant response among the transgenic lines which gave either a more susceptible or a more resistant response to the pathogen when compared with untransformed Désirée. Immunity toP. solanacearum was not observed, but it was possible to select a group of transgenic lines that showed resistance levels and disease development curves comparable to the field resistant cv. Cruza 148.
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  • 7
    ISSN: 1871-4528
    Keywords: post harvest disease ; potato ; Solanum tuberosum L. ; resistance ; toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Virulence of 12Fusarium oxysporum isolates was determined on three potato cultivars Late Harvest, BP1 and Kimberley Choice. The production of fusaric acid by the 12F. oxysporum isolates was also determined. The aim of the study was to determine whether a correlation exists between fusaric acid production and virulence ofF. oxysporum isolates. Late Harvest was the most tolerant and BP1 the most susceptible cultivar. Virulence ranking, order of theF. oxysporum isolates with the different cultivars corresponded with the mean of value of the ranking order for all three cultivars. A correlation was found between virulence of isolates and fusaric acid production except for Late Harvest. Fusaric acid production may play a major role in the development of dry rot in potato tubers.
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  • 8
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    Potato research 40 (1997), S. 317-326 
    ISSN: 1871-4528
    Keywords: Solanum spp. ; Globodera rostochiensis ; G. pallida ; biology ; pathotypes ; damage ; resistance ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A description of the biology of potato cyst nematodes,Globodera rostochiensis andG. pallida, and symptom development in potato crops is presented. This is followed by an overview of resistance and tolerance research and an explanation of how resistance derived fromSolanum andigena was deployed in integrated control schemes on sandy and sandy peat soils in the north-east of The Netherlands. The frequent use of this resistance source resulted in the predominance of ‘new’ pathotypes ofG. rostochiensis and ofG. pallida. The ability of the cyst nematodes to respond to new types of resistance with new pathotypes makes the search and breeding for resistance a continuing process. The absence of tolerance in exS. vernei (VTn)2 62-33-3 cultivars showed that resistance, if it is to be of use in severely infested fields, must be combined with an appreciable level of tolerance.
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  • 9
    ISSN: 1871-4528
    Keywords: antibiosis ; antixenosis ; Solanum sparsipilum ; S. pinnatisectum ; S. berthaultii ; glandular trichomes ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four introductions ofSolanum berthaultii wild species and sevenS. tuberosumxS. berthaultii hybrids were evaluated by antibiosis and antixenosis laboratory tests with potato tuber moth. Some morphological and chemical traits related to the trichomes were analysed. Two clones of the wild speciesS. sparsipilum and five ofS. pinnatisectum, characterised by high tuber resistance, were tested for leaf resistance, both by antibiosis and antixenosis assays. The genotypes with high density of trichomes A and with high exudate PPO activity, showed a moderate negative effect on pupal weight and fecundity in the antibiosis tests. Trichomes B exudate showed a strong repulsive effect on oviposition of adult in the antixenosis tests. TheS. pinnatisectum clones revealed a good leaf resistance level, whereas theS. sparsipilum clones showed lower levels of antibiosis and antixenosis.
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  • 10
    ISSN: 1871-4528
    Keywords: resistance ; potato breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Zusammenfassung Über Unterschiede in der Vermehrung des Kartoffelzystennematoden auf anfälligen Kartoffelsorten wurde schon früher kurz berichtet. Versuche, die im Scottish Crop Research Institute durchgeführt wurden und Ergebnisse aus Prüfungen von Zuchtmaterial haben ergeben dass im Hinblick auf die Resistenz gegen den Kartoffelzystennematoden phänotypische und genotypische Unterschiede zwischen den Klonen bestehen. Hier wird über Untersuchungen repräsentativer Sorten derSolanum tuberosum ssp.tuberosum Gruppe in bezug auf Variationen ihrer Anfälligkeit gegenGlobodera pallida berichtet. Tabelle 1 zeigt eine Reihe von allgemein als anfällig geltenden Kartoffelsorten, die mitG. pallida (Pa 2/3) geprüft wurden. Es wird über fünf Versuche berichtet; in den ersten drei (1A, 1B und 1C) wird die Resistenz von Klonen die aus Knollen aufgewachsen waren, erfasst und in den anderen zwei Versuchen (2A und 2B) die Resistenz von Sämlingen die aus Kreuzungen mit anfälligen Sorten stammen. Im versuch 1A (1978) wurden Augenstecklinge von 9 Sorten in 8 Wiederholungen im Gewächshaus ausgepflanzt. Der Kompost John Innes Nr. 2 wurde mit 40 Eiern/g vonG. pallida (Pa 2/3) inokuliert. Nach 11 Wochen wurden die Zysten ausgezählt und als Prozent der Zysten auf Pentland Crown (% Anfälligkeit) ausgedrückt (siehe Tabelle 1, erste Reihe und statistische Verrechnung in Tabelle 2a) Im Versuch 1B (1982) wurden 25 Sorten in fünf Wiederholungen in geschlossenen Behältern mit 4 verschiedenen Inokulumdichten geprüft: 1500, 2000, 2500 und 3000 Eier Behälter. Sieben Wochen später wurden die Zysten gezählt und als % Anfälligkeit, basierend auf den durchschnittlichen Zystenzahlen der 4 Inokulumdichten (Tab. 1, statistische Verrechnung in Tabelle 2b) ausgedrückt. Im Versuch 1 C (1983) wurden 22 Sorten bei einer Inokulumdichte von 3500 Eiern/Behälter geprüft. Die Ergebnisse zeigt Tabelle 1 und die Statistik Tabelle 2c. In Tabelle 1 sind die Sorten in absteigender Reihenfolge angegeben, beginnend mit P. Javelin, die die höchste Zystenzahl aufwies. Fünf der in Tabelle 1 angegebenen 36 Sorten wurden in allen drei Prüfungen verwendet: P. Javelin, Désirée, M. Piper, Croft und P. Crown. Tabelle 3 zeigt die Varianzanalyse der fünf Sorten und für drei Jahre. Die in den Tabellen 2a, 2b und 2c aufgeführten Analysen zeigen dass signifikante Unterschiede (P〈0,001) im Hinblick auf die Zystenbildung zwischen den Sorten bestehen. Die Analyse in Tabelle 3 veranschaulicht dass die signifikanten Unterschiede in bezug auf die Zahl der gebildeten Zysten auf die Haupteinflüsse (Jahre, Sorten) zurükzuführen ist. Im ersten Sämlingstest (2A) wurde die Resistenz von Nachkommenschaften eines North Carolina 2-Kreuzungsprogrammes untersucht, bei dem elf gegenG. pallida resistente Klone mit sieben anfälligen gekreuzt wurden. Tabelle 4 zeigt die durchschnittlichen Zystenzahlen für die Nachkommenschaften der anfälligen Sorten und in Tabelle 5 ist die statistische Verrechnung aufgeführt. Die Verteilung des allgemeinen Kombinationsfähigkeitseffektes (Tab. 5) zeigt dass die anfälligen Eltern regelmässig, aber mit unterschiedlichem Ausmass zum generellen, inherenten Resistenzgrad gegen Kartoffelzystennematoden in der Nachkommenschaft beitragen. Im zweiten Sämlingstest (2B) wurde die Resistenz gegenG. pallida in 4 Nachkommenschaften anfälliger Eltern untersucht. Diese wurden in 5×5×4,5 cm Plastiktöpfen ‘Plant Pak’ in John Innes Nr. 2 Kompost, inokuliert mit 40 Eiern/g vonG. pallida, untersucht. 24 Einzelsämlinge wurden in jedem von 4 Blöcken randomisiert, Abb. 1 gibt die nach neun Wochen ausgezählten sichtbaren Zystenzahlen wieder. Das zeigt dass in den 4 Nachkommenschaften eine Abstufung der Anfälligkeit vorhanden ist. Die Ergebnisse der fünf Versuche zeigen dass zwischen den ursprünglich als anfällig angesehenen Sorten Unterschiede im Ausmass der Zystenproduktion bestehen. Diese Unterschiede stellen sowohl phänotypische als auch genotypische Variationen dar, die vorher beiSolanum tuberosum ssp.tuberosum nicht deutlich aufgezeigt waren. Diese Unterschiede können auf verschiedenen Faktoren des Phänotyps, ausser dem Resistenzmechanismus an sich, beruhen; im Zusammenhang mit einem Züchtungsprogramm ist aber am wichtigsten dass sie eine vererbbare Komponente haben, die zur Beschleunigung der phänotypischen Ausprägung der Resistenz beitragen kann.
    Abstract: Résumé Les variations de taux de multiplication des nématodes à kyste de la pomme de terre pour des variétés sensibles ont déjà été brièvement relatées. Des essais conduits au Scottish Crop Research Institute et les données de tests de routine dans le programme de sélection montrent que des différences phénotypiques et génotypiques existent entre clones, en ce qui concerne la résistance du nématode à kyste. Les études présentées concernent les variétés représentatives du groupeSolanum tuberosum ssp.tuberosum pour leur variation de sensibilité auGlobodera pallida. Des variétés généralement connues pour leur sensibilité auG. pallida (Pa2/3) sont expérimentées et mentionnées dans le tableau 1. Cinq essais sont réalisés. Les 3 premiers (1A, 1B et 1C) concernant la résistance des clones à partir des plantes issues de tubercules tandis que les deux autres (2A et 2B) se rapportent à la résistance de descendants issus de plantules provenant de croisements avec des variétés sensibles. Dans l'essai 1A (1978), des oeilletons de neuf variétés sont plantés en serre, à raison de 8 répétitions par variété. Le compost John Innes No 2 est inoculé avec 40 oeufs/g deG. pallida (Pa2/3). Les comptages de kystes après 11 semaines, exprimés en pourcentage du nombre de kystes sur Pentland Crown (% sensibilité) sont présentés dans la première colonne du tableau 1 et l'analyse statistique figure dans le tableau 2a. L'essai 1B (1982) comporte 25 variétés répétées cinq fois, en containers clos, et inoculées à 4 niveaux: 1500, 2000, 2500 et 3000 oeufs par container. Les comptages de kystes après 7 semaines sont présentés dans le tableau 1 en pourcentage de sensibilité sur la base des comptages moyens de kystes pour les 4 niveaux d'inoculum et l'analyse dans le tableau 2b. L'essai 1 C (1983) comporte 22 variétés, également en containers clos, maís avec un seul niveau d'inoculum: 3500 oeufs par container. Les résultats sont présentés dans le tableau 1 et l'analyse dans le tableau 2c. Les variétés présentées dans le tableau 1 sont classées par ordre décroissant avec comme première variété P. Javelin, qui produit le plus grand nombre de kystes. Pour les 36 variétés présentées dans le tableau 1, tous les test ont été réalisés sur cinq d'entre elles: P. Javelin, Désirée, M. Piper, Croft et P. Crown. L'arrachage de variance pour les 3 années et les cinq variétés est indiquée dans le tableau 3. Les analyses dans les tableaux 2a, 2b et 2c démontrent que des différences significatives (P〈0,001) existent entre les variétés, quant à la production de kystes. L'analyse dans le tableau 3 montre que la variation significative n'est expliquée que par les facteurs principaux (années et variétés), en ce qui concerne le nombre de kystes. Le premier test sur plantules met en évidence la résistance de la descendance par un programme de croisement North Carolina-2 entre 11 clônes résistants àG. pallida et 7 variétés sensibles. Un résumé des moyennes des kystes dans la descendance des variétés sensibles est indiqué dans le tableau 4 et l'analyse dans le tableau 5. Le part des effets combinés possibles, dans le tableau 5, montre que les parents sensibles contribuent de façon notable, mais à des degrés différents, aux niveaux de résistance propre au nématode pour chaque descendant. Le deuxième test sur plantules met en évidence la résistance àG. pallida pour quatre descendants de variétés sensibles. L'étude est faite dans des pots en plastique ‘plant pak’ de 5×5×4,5 cm remplis de compost John Innes No 2 inoculés avec 40 oeufs/g deG. pallida. 24 plantules individuelles sont placées au hasard dans chacun des quatre blocs. Les kistes visibles sont comptés après 9 semaines (figure 1); les résultats indiquent que différents niveaux de sensibilité existent pour les quatre descendants. Les résultats des cinq expériences démontrent qu'il y a des différences dans les niveaux de production de kystes pour des variétés prealablement classées comme étant d'égale sensibilité. Ces différences représentent des variations à la fois phénotypique et génotypique, au départ non clairement identifiées avecS. tuberosum ssp.tuberosum. Ces différences peuvent provenir de facteurs divers du phénotype autres que les mécanismes de résistance véritables, cependant il est important de considérer ce facteur héréditaire dans un programme de sélection ou il peut être utilisé pour accroître la résistance phénotypique.
    Notes: Summary Many cultivars of theSolanum tuberosum ssp.tuberosum group were investigated in a number of studies for their susceptibility toGlobodera pallida. Phenotypic and genotypic variation for this character is reported in material previously regarded as uniformly susceptible. The implications of these results are discussed in relation to potato breeding policy and nematological studies.
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  • 11
    ISSN: 1871-4528
    Keywords: common scab ; Streptomyces scabies ; potato ; genotype ; resistance ; environment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Zusammenfassung Die Knollen wurden auf ihre Anfälligkeit für Flachschorf bestimmt, indem der Bedeckungsgrad geschätzt und der schlimmste Schorftyp auf den einzelnen Knollen notiert wurden. Der Bedeckungsgrad wurde anhand einer Skala bestimmt, wobei 0=kein Schorf, 1=bis zu 10% der Oberfläche von Schorf befallen und jede weitere Einheit eine Zunahme von 10% bedeuten. Der Schorftyp wurde anhand einer Skala von 0–5 bonitiert: 0 = keine Schorfarten, 1 = wenig, oberflächliche Läsionen, 2 = gross, oberflächliche Läsionen, 3 = gross, rauhe Läsionen, 4 = grosse Läsionen, entweder aufgeworfen oder flachnarbig, 5 = grosse Läsionen, tiefnarbig. Die Mittelwerte für die Parzellen wurden errechnet. Das Verhältnis zwischen dem mit Flachschort befallenen Umfang der Knollenoberfläche und der Schwere des Schorftypes wurde untersucht, indem für acht Versuche an zwei Orten über zwei Vegetationsperioden (Abb. 1) Regressionen für den Umfang pro Typ errechnet und die Homogenität der Regressionen durch Varianzanalysen (Tabelle 1) verglichen wurden. Alle Regressionskoeffizienten waren hoch signifikant und wichen nicht signifikant voneinander ab. Die Tatsache, dass der Schorfbefall sich im gleichen Mass wie der Schorftyp verändert, weist darauf hin, dass — innerhalb der untersuchten Population von Genotypen — die Resistenz der Kartoffel gegen das Eindringen von sich entwickelnden Lentizellen und die Besiedlung von Knollengewebe eng zusammenhängen. Die Interaktion des Kartoffelgenotyps mit der Umwelt kann manchmal Knollen mit nur wenigen, tiefen Läsionen, wie im Versuch mit frühen Sorten in Trumpington 1975 (Abb. 11), oder mit zahlreichen oberflächlichen Schorfen, wie im Versuch mit mittelfrühen bis mittelspäten Sorten in Trumpington 1976 (Abb. 17), bewirken. Es ist daher nicht angezeigt, nur aufgrund des Vorhandenseins von oberflächlichen oder normalen Schorfen eine Auslese auf Resistenz oder Anfälligkeit zu treffen. Obwohl alle Regressionskoeffizienten hoch signifikant waren, gab es doch einzelne Abweichungen von den Regressionslinien. Ein Beispiel ist der in Abb. 15,6 mit × bezeichnete Klon, der eine kleinere Befallsfläche aufwies als 1976 an beiden Orten zu erwarten gewesen wäre.
    Abstract: Résumé On a étudié la sensibilité des tubercules à la gale commune par estimation de la surface atteinte et par notation du type de gale le plus grave sur chaque tubercule. On a établi une échelle de 1 à 10 en fonction du degré d'attaque, ou 0=pas de gale, 1=jusqu'à 10% de la surface atteinte. A chaque unité supplémentaire correspond une augmentation de 10%. Une échelle de 0 à 5 a été utilisée pour le type de gale; 0=pas de gale, 1=petites lésions superficielles, 2=grandes lésions superficielles, 3=grandes lésions rugueuses, 4=grandes lésions en relief ou en creux peu profonds, 5=grandes lésions en creux profonds. On a calculé des moyennes pour chaque parcelle. La relation entre la surface du tubercule couvert de lésions et la sévérité du type de gale a été examinée par calculs de régressions entre ces deux facteurs à partir de huit essais situés à deux endroits durant deux saisons (fig. 1) et par un test d'homogénéité des coefficients de régression par analyse de variance (tableau 1). Tous les coefficients de régression ont été hautement significatifs sans être différents de manière significative entre eux. Le fait que la surface des lésions change de manière uniforme à l'intérieur de la population des génotypes testés, conduit l'auteur à penser que la résistance des pommes de terre à la pénétration par les lenticules formées et la colonisation des tissue du tubercule sont intimement liées. L'interaction entre le génotype et l'environnement peut quelquefois produire des tubercules ayant seulement quelques lésions profondes, comme cela s'est produit dans l'essai récolté tôt de Trumpington en 1975 (fig. 11), où de nombreuses lésions superficielles, comme pour l'essai de Trumpington en 1976 (fig. 17) récolté plus tardivement. Par conséquent, dans le cadre d'un programme de sélection, il est insuffisant de classer des plantes résistantes ou sensibles uniquement à partir de gales respectivement superficielles ou normales. Bien que tous les coefficients de régression soient hautement significatifs il y a des déviations individuelles à partir des courbes de régression. Un exemple est donné par le clone marqué d'une croix (×) dans la fig. 15,6 lequel avait une surface atteinte plus petite que ce que l'on pouvait expecter pour les deux endroits en 1976.
    Notes: Summary The relationship between the area of tuber surface covered with lesions of common scab and the severity of the scab type was examined by calculating regressions of cover on type for eight trials at two sites over two seasons. All the regression coefficients were highly significant and did not differ significantly from each other. This suggests that resistance to penetration and to colonization of tubers by the parasite are closely related. Differences in the position of regression lines were probably due to local environmental conditions. The interaction of the potato genotype with the environment can result in relatively few severe scabs or many superficial scabs.
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  • 12
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    Potato research 28 (1985), S. 27-33 
    ISSN: 1871-4528
    Keywords: infection experiments ; latent infection ; resistance ; symptom development ; ring rot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Zusammenfassung Wurzeln von gekeimten Saatknollen der Sorten Ostara, Laila, Kerrs Pink und Pimpernel wurden durch Tauchen in Suspensionen vonCorynebacterium sepedonicum inokuliert. Dies erbrachte einen beträchtlichen und signifikanten Ertragsrückgang, vor allem bei der Sorte Pimpernel (Tabelle 1); desweiteren zeigte sich eine signifikante Sorte/Infektion-Wechselwirkung. Inokulation ergab, mit Ausnahme von Laila, eine signifikante Senkung der durchschnittlichen Knollenzahl (Tabelle 2); am grössten war der Rückgang bei Kerrs Pink, während das durchschnittliche Knollengewicht am meisten zurückging bei Pimpernel (Tabelle 3). Der Ertragsrückgang bei Laila erfolgte durch Reduktion des durchschnittlichen Knollengewichtes, bei den anderen Sorten jedoch durch Rückgang der durchschnittlichen Knollenzahl. Diese Beobachtung lässt darauf schliessen dass die Entwicklung der Infektion bei Laila nach der Knollen-Initiierung einsetzte, während sie bei den anderen Sorten schon früher einsetzte und somit die Knollenzahl beeinflusste. Im Gegensatz zu den anderen Sorten zeigten einige Tochterknollen von Laila äussere Symptome, in Verbindung mit starker Fäule, während in der Entwicklung interner Symptome nur geringe Unterschiede zwischen den Sorten gefunden wurden (Tabelle 4). Dennoch waren die Konzentrationen vonC. sepedonicum in den meisten symptomlosen Tochterknollen inokulierter Pflanzen hoch (Tabelle 4). Die Ergebnisse zeigen dass die Sorte Laila gegen eineC. sepedonicum-Infektion resistenter als die anderen geprüften Sorten ist, dass jedoch hohe Konzentrationen des Erregers in symptomlosen Knollen von inokulierten Pflanzen gegen eine Verwendung bei der Züchtung sprechen.
    Abstract: Résumé Des racines de tubercules de semence germés (variétés Ostara, Laila, Kerrs Pink et Pimpernel) sont inoculées par immersion dans des suspensions deCorynebacterium sepedonicum. Cela provoque une diminution conéquente et significative du rendement, en particulier pour la variété Pimpernel (tableau 1), et il existe une interaction significative variété/infection. L'inoculation diminue significativement le nombre moyen de tubercules (tableau 2) sauf pour la variété Laila; la diminution est la plus importante pour la variété Kerrs Pink, tandis que le poids moyen de tubercules diminue le plus pour la variété Pimpernel (tableau 3). La diminution du rendement pour la variété Laila est provoquée par une réduction du poids de tubercules. Cette observation suggère que le développement de l'infection pour la variété Laila débute après l'initiation de tubérisation, alors que pour les autres variétés, elle commence plus tôt et de ce fait influence le nombre de tubercules. Peu de tubercules fils de la variété Laila montrent des symptômes externes associés à une pourriture importante en comparaison avec les autres variétés, alors que de petites différences s'observent entre toutes les variétés au niveau du développement de symptômes internes (tableau 4). Les concentrations deC. sepedonicum sont cependant élevées dans la plupart des tubercules fils ne présentant pas de symptômes et provenant de plantes inoculées (tableau 4). Les résultats montrent que la variété Laila est plus résistante à l'infection deC. sepedonicum que les autres variétés testées, mais les fortes concentrations du pathogène dans les tubercules sans symptôme provenant de plantes inoculées empêchent son utilisation en multiplication.
    Notes: Summary Inoculating the roots of potato cvs Ostara, Laila, Kerrs Pink, and Pimpernel withCorynebacterium sepedonicum caused severe rotting of tubers and between 12 and 51 % decrease in yield. There was a significant cultivar/infection interaction. The numbers of tubers decreased significantly except for cv. Laila, which also showed little rotting of tubers, and the average tuber weight decreased most in cv. Pimpernel. The concentration ofC. sepedonicum was high in most inoculated tubers including those not showing symptoms.
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  • 13
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    Potato research 30 (1987), S. 1-8 
    ISSN: 1871-4528
    Keywords: resistance ; latent infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Zusammenfassung Es wurden 13 Kartoffelsorten auf das Vorhandensein vonPhoma exigua var.foveata in Stengeln und grünen Blättern untersucht. Pflanzknollen, die nach künstlicher Infektion faulten, wurden im Feld ausgepflanzt; nach 5, 12 und 17 Wochen wurden pro Sorte vier Stengel mit Blättern nach dem Zufallsprinzip ausgewählt, wobei jeder Stengel eine Pflanze repräsentierte. Die Stengel wurden gewaschen, getrocknet und in 5 cm-Segmente geteilt. Nach Oberflächensterilisation wurden die Segmente und die Blattschnitte in Petrischalen auf ein selektives Agarmedium gebracht, das zur Förderung der Pyknidienentwicklung Paraquat enthielt.P. exigua var.foveata wurde auf Malzextraktagar bestimmt, der Thiophanatmethyl und Chloramphenicol enthielt. Die Sorten reagierten unterschiedlich auf die Infektion, wie an der Anzahl der Schnitte, die das Pathogen lieferten, zu ersehen ist. Die Nachweishäufigkeit war am niedrigsten bei der ersten und am höchsten bei der letzten Probenahme (Tab. 2). Die mittleren Prozentwerte varierten von 13% bis 36%, die Reihenfolge war zwischen den Jahren ähnlich (Tab. 1). Das Verteilungsmuster der Schnitte, die das Pathogen lieferten, war unregelmässig; es wurde überall in Stengelschnitten und Blättchen vom oberirdischen Trieb nachgewiesen und in nur wenigen Fällen auch in Stengelsegmenten unterhalb der Bodenoberfläche (Tab. 3). Die Werte zeigen, dass die Anfälligkeit der Sorten zur symptomlosen Invasion der grünen Triebe differiert. Inwieweit in dieser Phase des Lebenszyklus des Pilzes eine Resistenzauslese lohnend ist, hängt von der möglichen Bedeutung des Stengelinokulums als Quelle für die Infektion der Knollennachkommenschaft ab. Es war mit dieser Untersuchung nicht beabsichtigt, Fragen, die sich auf die Ausbreitung des Pilzes beziehen, zu beantworten; ein besseres Verständnis der Biologie dieses Krankheitserregers ist aber entscheidend zur rationalen Anwendung der Ergebnisse in der Pflanzenzüchtung.
    Abstract: Résumé La présence dePhoma exigua var.foveata dans les tiges et les feuilles vertes est étudiée sur treize variétés de pommes de terre. Des tubercules de semence pourris après contamination artificielle, sont plantés au champ. Après 5, 12 et 17 semaines, 4 tiges avec les feuilles de chaque variété sont prélevées au hasard, chaque tige représentant une plante. Les tiges sont lavées, séchées et coupées en segments de 5 cm. Après une stérilisation de surface les segments et des sections de folioles sont placés en boites de pétri sur un milieu sélectif à base d'agar contenant du paraquat afin de favoriser le développement des pycnides.Phoma exigua var.foveata est identifié sur malt agar contenant du thiophanate-méthyl et chloramphenicol. Les variétés répondent différemment à l'infection, comme le montre le nombre de sections hébergeant le pathogène. La fréquence de détection est la plus faible à la première date d'échantillonnage et la plus élevée à la dernière (tableau 2). Le pourcentage moyen varie entre 13 et 36%, le classement est similaire entre les années (tableau 1). La distribution du pathogène hébergé par les échantillons est irrégulière. Il est détecté sur des sections de tiges et des folioles au dessus du sol et dans quelques cas sur des segments de tiges en dessous de la surface du sol (tableau 3). Les données montrent que la sensibilité des variétés à l'infection des parties aériennes est variable. L'intérêt d'une sélection à la résistance pour cette phase du cycle du champignon dépend de l'importance potentielle de l'inoculum sur tige en temps que source de contamination des tubercules-fils. Cette étude n'a pas pour but d'expliquer la dissémination du champignon, mais une meilleure connaissance de la biologie du pathogène est fondamentale pour une application rationnelle des résultats de la multiplication variétale.
    Notes: Summary Thirteen cultivars were tested for presence ofPhoma exigua var.foveata in stems and green leaves. The frequency of segments yielding the pathogen increased with time during the growing season and varied between cultivars from 13 to 36% and their distribution pattern was irregular. There were significant differences between cultivars to this symptomless invasion of the green shoots. The value of such cultivar differences depends on the interpretation of the importance of stem inoculum in carrying the disease to the next tuber generation.
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    Potato research 39 (1996), S. 395-401 
    ISSN: 1871-4528
    Keywords: germplasm ; TPS ; resistance ; rapid multiplication ; virus ; collaborative research ; research priorities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary After an initial phase of development and programme establishment the activities of the International Potato Center (CIP) grew rapidly from 1981 to 1985, putting emphasis on germplasm distribution, research on diffused-light storage and TPS, and exploration of sources of resistance. Later, strong emphasis was given to genetic resource utilization and field resistance to late blight. The impact of CIP research showed internal rates of return ranging from 26 to 102 percent. At the end of the 1980s CIP's financial resources were cut severely and restructuring the programme was necessary. Through a careful process of prioritizing, programmes are identified which will contribute most to ensuring the continued increase of potato production in developing countries and to continuing the leading role of CIP in potato research.
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  • 15
    ISSN: 1572-9788
    Keywords: Helianthus annuus ; QTL ; resistance ; RFLP ; Sclerotinia scerotorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Restriction fragment length polymorphism and isoenzyme markers were used to investigate quantitative trait loci involved in sunflower resistance to mycelial extension of Sclerotinia sclerotiorum on leaves and capitula. Seed weight, oil content and flowering data were also evaluated. Four quantitative trait loci were demonstrated for leaf resistance and two for capitulum resistance. One of these zones appears involved in resistance to both types of S. sclerotiorum attack while the others appear specific for resistance of one part of the plant. Two quantitative trait loci were detected for seed weight, three for oil content and three for flowering date. Individual quantitative trait loci explained 9% to 48% of the phenotypic variability, confirming the polygenic basis of the quantitative traits studied. Overall, the quantitative trait loci explain 60% of the genetic variation for leaf resistance and 38% for capitulum resistance to S. sclerotiorum. One linkage group is particularly interesting since it includes quantitative trait loci for all the five quantitative traits measured. Hypotheses for linkage versus pleiotropy and consequences of all the results in resistance breeding are discussed.
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  • 16
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    Molecular breeding 3 (1997), S. 213-217 
    ISSN: 1572-9788
    Keywords: geminiviruses ; beet curly top virus ; DI DNA ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Beet curly top virus (BCTV) infection is associated with the de novo synthesis of a heterogeneous population of subgenomic viral DNAs. Nicotiana benthamiana plants transformed with a partial repeat of one such subgenomic DNA remained susceptible to infection but produced ameliorated symptoms when agroinoculated with BCTV. Symptom amelioration is associated with the mobilization of subgenomic DNA from the integrated copy. In an attempt to improve the resistance, N. benthamiana has been transformed with a partial repeat of a much smaller subgenomic DNA. However, transgenic plants showed almost no resistance although subgenomic DNA was mobilised from the host genome. To further understand the molecular basis of the interference phenomenon, we compared the ability of BCTV to replicate and accumulate in leaf discs derived from resistant and non-resistant transgenic plants. Both subgenomic DNAs were able to interfere with virus replication but only in case of resistant plants the DI DNA efficiently suppressed viral accumulation.
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  • 17
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    European journal of plant pathology 102 (1996), S. 773-778 
    ISSN: 1573-8469
    Keywords: apple scab ; environmental factors ; resistance ; Venturia inaequalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The susceptibility of apple leaves toVenturia inaequalis was investigated by assessing disease on individual leaves of seedlings and labelled shoots of orchard trees. Four sets (A-D) of potted seedlings of cv. ‘Golden Delicious’, which had been grown in a glasshouse, each with approximately 30 mature leaves, were exposed to a high-inoculum orchard. Sets A and B were exposed after each other for 47 and 42 days, respectively. As a result of the six and three infection periods during exposure, 94% and 81%, respectively of the seedling shoot tips in set A and set B became infected. However, due to ontogenetic resistance, disease incidence was low in both sets on leaves which were fully expanded at the beginning of exposure. Set C was exposed during both periods (89 days) in which sets A and B were exposed. Not only were all the seedling shoot tips in set C infected, but also — due to the loss of ontogenetic resistance — nearly all of the mature leaves. Ontogenetic resistance was also lost in set D, which was exposed for 57 days at the end of the growing season. The symptoms on fully expanded leaves on plants in sets C and D were typical and similar to those on young leaves. A time course symptom assessment was performed on leaves which had developed early in the season on labelled, field-grown shoots of cv. ‘Golden Delicious’ trees. A steady increase of disease incidence was detected, which could not only have resulted from infections followed by extended incubation periods. In addition to the increase of typical lesions on both leaf surfaces, there was also an abundance of diffuse mycelial development on the lower surfaces of the leaves of the field-grown trees.
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  • 18
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    European journal of plant pathology 103 (1997), S. 653-665 
    ISSN: 1573-8469
    Keywords: avoidance ; Melampsora pinitorqua ; path coefficient analysis ; Pinus pinaster ; resistance ; rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Data collected in a three-year field experiment on infection and damage by Melampsora pinitorqua on maritime pines showing contrasting growth were analysed by means of path analysis. The severity of infection in 2 year-old pines could be mainly explained by the amount of susceptible tissues (elongating shoots). In contrast, two years later, ‘intrinsic susceptibility’, i.e. expressed after inoculation, was shown to have the most important direct effect on the amount of infection. Intrinsic susceptibility could be satisfactorily assessed by artificial inoculations on excised shoots under controlled conditions. Shoot potassium and phosphorous contents were shown to have marked positive effects on susceptibility. The hypothesis of an age effect on the relative importance of different resistance mechanisms, i.e. avoidance and active resistance, in maritime pine interaction with twisting rust is discussed.
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  • 19
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    European journal of plant pathology 104 (1998), S. 11-15 
    ISSN: 1573-8469
    Keywords: black pod ; resistance ; Theobroma cacao
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two species of Phytophthora (P. palmivora and P. capsici) and inoculations at two depths (3 mm and 9 mm) were tested each on 10 clones of Theobroma cacao to determine their effects on pod resistance. Ripe and unripe pods were also assessed to determine the influence of physiological status of the pod on the expression of resistance. The two pathogens tested (P. palmivora and P. capsici) differed significantly in their reactions on pods, with P. palmivora being more aggressive than P. capsici. However, the lack of interaction between clones and pathogen species and the similarity in the ranking of clones based on lesion size suggested that selection for resistant clones can be based on one of the two pathogens, preferably the more aggressive one. Pod reactions differed between inoculation depths (3 mm and 9 mm), and between pod maturity stages (ripe and unripe pods) with relatively larger lesions being recorded at 9 mm depth and on unripe pods as compared to those observed at 3 mm depth and on unripe pods, respectively. The magnitude of increase in lesion sizes, however, varied with genotypes, indicating that inoculation depth and pod maturity stage should be standardized in screening cacao germplasm for resistance to Phytophthora.
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  • 20
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    European journal of plant pathology 104 (1998), S. 449-455 
    ISSN: 1573-8469
    Keywords: lettuce ; lettuce downy mildew ; Bremia lactucae ; phenylamide fungicides ; virulence factors ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Control of lettuce downy mildew (Bremia lactucae) with phenylamide fungicides has failed in some intensive lettuce-producing areas in Northern Italy since Spring 1993. Before then, these chemicals and particularly metalaxyl, provided the best disease control. The sensitivity of Bremia lactucae isolates collected in such areas to metalaxyl was evaluated in the laboratory. These strains grew and sporulated profusely on lettuce seedlings treated with 100 and 200 ppm of metalaxyl, whereas sensitive control strains were completely inhibited when treated with fungicide concentrations ranging from 0.5–1 ppm. Thus in Italy occurrence of resistance to phenylamide fungicides in Bremia lactucae has also been demonstrated, as in almost all the countries where these chemicals were previously authorised. Subsequently, a demonstration of occurrence of resistance was made and the virulence pattern of several strains (resistant and sensitive to metalaxyl) was characterised using differential NL series containing the 13 DM resistant genes or R factors. The results suggest the occurrence of a new pathotype in Italy different from all the 16 NL Bremia lactucae races studied.
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    European journal of plant pathology 105 (1999), S. 431-443 
    ISSN: 1573-8469
    Keywords: basal rot ; cell wall degradation ; Lilium ; pathogenesis ; plasmolysis ; resistance ; transfer cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The early interaction of lily roots with the cortical rot pathogen Fusarium oxysporum f.sp. lilii was studied using roots of lily bulblets grown in Hoagland's solution, inoculated with the pathogen, and sampled up to 48 h later. Conidia produced germ tubes within 6 h, which extended towards and into the mucilage covering the root elongation zone, and along and into the anticlinal grooves and middle lamellae of epidermal cells. By 24–48 h, infecting hyphae had reached the periclinal walls and intercellular spaces between the epidermis and the outermost cells of the cortex. Penetration of intercellularly growing hyphae directly across host cell walls was not observed; invasion of the cell lumen only occurred by gradual infringing of hyphae upon successive primary wall layers. Non-cellulosic wall appositions rich in vesicles and covered by a cellulosic protective-like layer were formed in response to approaching hyphae in resistant cv.Connecticut King, but rarely in susceptible cv. Esther which seemed more susceptible to plasmolysis and rot. Finger-like projections of the appositions into the host cell cytoplasm likely represent early stages of transfer cell formation.
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  • 22
    ISSN: 1573-8469
    Keywords: Ceratocystis fimbriata ; phytoalexins ; Platanus spp. ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inoculation of leaves of resistantPlatanus occidentalis and susceptiblePlatanus acerifolia leaves withCeratocystis fimbriata f. sp.platani, the canker stain disease agent, induced foliar necrosis and biosynthesis of two phytoalexins, scopoletin and umbelliferone. Foliar symptoms keep localized and accumulation of coumarin phytoalexins was rapid for incompatible interactions. Necrosis spread widely and accumulation of these phenolic compounds was much later and lower for compatible interactions. The differential response could be used in a genetic improvement program for resistance against canker stain.
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  • 23
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    European journal of plant pathology 102 (1996), S. 311-316 
    ISSN: 1573-8469
    Keywords: Capsicum annuum ; C. frutescens ; resistance ; Phytaphthora capsici ; host-pathogen interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pepper cultivars from diverse geographic origins were evaluated for resistance to different isolates ofPhytophthora capsici under controlled environmental conditions. All accessions tested were susceptible at the four-leaf stage to the six isolates ofP. capsici. Inoculation at the eight-leaf stage resulted in significantly different interactions among the accessions andP. capsici isolates. The Korean and U.S. cultivars tested were highly susceptible to the isolates ofP. capsici at this stage. In contrast, PI 201234 and PI 201238 had a differential interaction with someP. capsici isolates. At the twelve-leaf stage, Phytophthora blight developed slowly in the Korean and U.S. cultivars that were highly susceptible at the eight-leaf stage. Furthermore, the accessions from the Asian Vegetable Research and Development Center (AVRDC) became highly resistant toP. capsici at this stage.
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  • 24
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    Plant and soil 113 (1989), S. 121-124 
    ISSN: 1573-5036
    Keywords: CAT scanning ; drawdown ; plant roots ; resistance ; water uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using the technique of Computer Assisted Tomography applied to gamma ray attenuation measurement of soil water content, it has been shown that the assumption of uniform absorption of soil water along a plant root is clearly erroneous and that drawdown distance is a function of time. The results suggest that the plant sequentially removes water from the top to the bottom of the root as soil hydraulic resistance becomes a major limiting factor in the upper layers, even at the high soil water potential (−0.30 MPa) used.
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  • 25
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    European journal of plant pathology 103 (1997), S. 15-23 
    ISSN: 1573-8469
    Keywords: breeding ; Dianthus caryophullus ; Fusarium oxysporum ; pectic enzymes ; phytoalexins ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Carnation cultivars with different levels of partial resistance were inoculated with race 2 of Fusarium oxysporum f.sp. dianthi and monitored for accumulation of host phytoalexins, fungal escape from compartmentalization, production of fungal pectin-degrading enzymes and development of external disease symptoms. Accumulation of phytoalexins, assessed after 10 days in the first 5 cm above the inoculation site, was weakly (methoxydianthramide S) or not (hydroxydianthalexin B) correlated with resistance levels after 12 weeks. Fungal escape from compartmentalization, assessed after 3 weeks as percentages colonized plants at 8 cm above the inoculation site, was highly correlated with expression of susceptibility after 12 weeks. Polygalacturonase (PG) activity, assessed after 4 weeks in the first 5 cm above the inoculation site, was highly correlated to final disease development. Linear increases in disease severity were accompanied by quadratic increases in PG activity. In contrast to water-treated plants, that lacked any PG activity, inoculated plants contained two main groups of fungal PGs, the dominant forms of which had estimated pI values of 7.0 and minimally 9.5, respectively. Compared to those of the first group, enzymes of the second group were produced only in trace amounts in liquid media containing pectin or polygalacturonate as sole source of carbon. On these media, the fungus also produced a pectin methyl esterase (PME) with an estimated pI of 9.3. Besides PMEs of host origin, inoculated plants of susceptible cultivars contained the fungal PME while no more than traces were found in resistant ones. Assessment of phytoalexin production by the host during defense responses cannot replace monitoring of external symptoms as a resistance test. Assessment of fungal growth, whether by reisolations above the compartmentalization area or by measurement of PG activity, provides a both rapid and reliable prediction of disease development.
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  • 26
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    European journal of plant pathology 105 (1999), S. 509-512 
    ISSN: 1573-8469
    Keywords: tospovirus ; resistance ; Capsicum sp. ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Infection by two isolates of impatiens necrotic spot tospovirus (INSV) under temperature regimes of 25/18°C (day/night) or 33°C (continuous) was studied in Capsicum annuum (systemically susceptible to tomato spotted wilt tospovirus, TSWV), C. chinense PI 152225 and PI 159236 (reacting hypersensitively to TSWV) and Nicotiana benthamiana (systemically susceptible to both tospoviruses). At 25/18°C infection was systemic in all hosts tested. At 33°C infection in N. benthamiana was systemic whereas in C. annuum and C. chinense it was restricted to the inoculated leaves. The result differed from that reported for TSWV, where high temperature made plants more susceptible, or caused no difference. Exchanging temperature regimes 6 h to 4 days after inoculation did not affect the final results one month later, with plants being only locally infected at 33°C continuous regime, or systemically infected at 25/18°C alternate regime. The two INSV isolates were biologically and serologically stable for 5 passages in N. benthamiana held continuously at 33°C.
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  • 27
    ISSN: 1573-8469
    Keywords: Phytophthora infestans ; potato ; wild Solanum spp. ; resistance ; detached leaves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Physiological and molecular research on resistance responses of Solanum tuberosum cultivars and partially resistant Solanum species to Phytophthora infestans requires a reliable resistance test that can be used in the laboratory. Laboratory tests performed on detached leaves and intact plants were compared with field tests for similarity of late blight reactions. Detached leaves from field-grown plants were as resistant as detached leaves from climate chamber-grown plants when challenged with P. infestans. However, detached leaves incubated in covered trays at high relative humidity were more susceptible than detached leaves kept in open trays or leaves on intact plants. The incubation conditions of detached leaves in covered trays rather than detachment itself appeared to affect the resistance expression. Detached leaves of some wild Solanum genotypes became partially infected, whereas intact plants were completely resistant when inoculated. Inoculation of leaves on intact plants, however, resulted in lower infection efficiencies. These limitations should be taken into account when choosing the appropriate inoculation method for specific purposes. For resistance screening, laboratory tests proved to be a good alternative for field tests. The ranking of resistance levels for twenty plant genotypes was similar under laboratory and field conditions.
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    European journal of plant pathology 101 (1995), S. 51-56 
    ISSN: 1573-8469
    Keywords: resistance ; potato breeding ; roots canister test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fourteen cultivars generally regarded as uniformly susceptible toGlobodera pallida Pa 2/3 were assessed in plastic closed containers for degree of susceptibility and also for root and sprouting characteristics. The material was assessed by scoring degree of sprouting before planting and number of cysts and root growth at the end of experiment at intervals from the time of initial dormancy break through to prolific sprouting. Using covariate analyses, it was found that the degree of root development, particularly in the earlier assessments, significantly influenced the degree of cyst development and so the perceived levels of susceptibility/resistance. Having taken account of root growth characters, significant differences between the cultivars with regard to nematode multiplication were still evident. The implications of the results are discussed in relation to potato breeding and nematological studies.
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    European journal of plant pathology 95 (1989), S. 343-356 
    ISSN: 1573-8469
    Keywords: centre of virus origin ; hypersensitivity-associated resistance ; immunity ; resistance ; resistance sources ; sensitivity ; test plants ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Na inoculatie van een grote collectieNicotiana-soorten met 14 isolaten van de aardappelvirussen A (PVA), X (PVX) en Y (PVY) en tabaksmozaïekvirus (TMV), konden de secties of sectiedelen van het genus op basis van de gemiddelde reactie van hun soorten in vijf groepen worden verdeeld. Soorten uit Australische woestijngebieden en taxonomisch behorend tot de sectieSuaveolentes (subgenusPetunioides) bleken het meest gevoelig en het minst resistent, terwijl de sectiesPaniculatae (subgenusRustica),Tomentosae (Tabacum) enNoctiflorae (Petunioides) het minst gevoelig en het meest resistent waren. Van de collectienummers van de laatstgenoemde secties bleek 61% resistentie tegen minstens twee van de virussen te bezitten. De meest resistente soorten hebben hun verspreidingsgebied vooral in het centrale deel van het Andesgebergte (het zuidelijk deel van Peru, Bolivia en noord-westelijk Argentinië). Mogelijk is dit het ontstaansgebied van deze virussen. De einge andere resistente soort wasN. africana (Suaveolentes) uit zuid-westelijk Afrika. De meest gevoelige Amerikaanse secties, te wetenGenuinae (Tabacum) enTrigonophyllae, Alatae, Acuminatae enBigelovianae (Petunioides), waren gemiddeld gevoeliger danSuaveolentes-soorten die niet uit de Australische woestijnen afkomstig zijn. Het resterende deel van het genus, bestaande uit de Amerikaanse sectiesThyrsiflorae enRusticae (Rustica) enUndulatae, Repandae enNudicaules (Petunioides), stond met betrekking tot gevoeligheid en resistentie tussen laatstgenoemd deel van de sectieSuaveolentes en de groep resistente secties in, maar vertoonde in hoge mate tolerantie voor PVA, PVX en PVY en met overgevoeligheid samengaande resistentie voor TMV. Uit de resultaten blijkt dat de reacties van het genusNicotiana op virussen vooral geografisch bepaald zijn en in mindere mate taxonomisch.
    Notes: Abstract When testing the response of species of the genusNicotiana to 14 isolates of potato viruses A (PVA), X (PVX) and Y (PVY) and tobacco mosaic virus (TMV), sections and section parts of the genus could be divided into five groups according to the overall reaction of their species. Species from arid regions of australia and belonging to the sectionSuaveolentes (subgenusPetunioides) were most sensitive and least resistant, whereas the sectionsPaniculatae (subgenusRustica),Tomentosae (Tabacum) andNoctiflorae (Petunioides) appeared least sensitive and most resistant. Sixty-one percent of the accessions of the latter sections proved resistant to at least two of the viruses. The most resistant species have their main geographical distribution in the central Andes (southern Peru, Bolivia and north-western Argentina), where the viruses may have originated. One other resistant species wasN. africana (Suaveolentes) indigenous to south-western Africa. The most sensitive American sections, viz.Genuinae (Tabacum) andTrigonophyllae, Alatae, Acuminatae andBigelovianae (Petunioides), were generally more sensitive than species of theSuaveolentes section from outside the arid regions of Australia. The remaining part of the genus, viz. the American sectionsThyrsiflorae andRusticae (Rustica) andUndulatae, Repandea andNudicaules (Petunioides) was intermediate between the latter group ofSuaveolentes species and the resistant group of sections with regard to sensitivity and resistance, but had a high rate of tolerance to PVA, PVX and PVY and of hypersensitivity-associated resistance to TMV. The results indicate that the viral respons in the genusNicotiana is mainly determined geographically and to a lesser extent taxonomically.
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  • 30
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    Euphytica 100 (1998), S. 45-50 
    ISSN: 1573-5060
    Keywords: incidence ; Karnal bunt ; occurrence ; resistance ; variability ; wild relatives ; progenitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bunts [common bunt (Tilletia laevis and T. tritici) and dwarf bunt (T. controversa)] and smuts [loose smut (Ustilago tritici) and flag smut (Urocystis agropyri)] of wheat are important cereal diseases in most countries of north Africa and the Near East. There are no peculiarities in the occurrence of bunts and smuts, except for dwarf bunt, which is limited to high-altitude areas of Turkey, Iran and Iraq. Host adaptation, rather than topography, determines the distribution of the common bunt pathogens: T. laevis predominates in bread wheat, whereas T. tritici attacks both bread and durum wheat non-preferentially. Incidence of bunt- and smut-affected wheat heads is generally low, however the frequency of their occurrence in fields is high, indicating their potential threat. Some bunts and smuts occur on hosts other than wheat, for example, flag smut on Aegilops crassa, loose smut on Ae. geniculata and rye, and dwarf bunt on Hordeum and Aegilops spp. Genetic variability of the pathogens causing common bunt and loose smut was investigated. Chemical seed treatment is the most widely used control for bunts and smuts. Organic nutrients as seed treatments to control common bunt of wheat show considerable promise. Sources of resistance to loose smut, and common and dwarf bunts of wheat, are available in wheat and its wild relatives. Triticum boeoticum, T. dicoccoides, and Aegilops species represent excellent sources of resistance to common bunt. There are three major sources of resistance in durum wheats, Senatore Cappelli and Haurani, Jenneh Khetifa, and Mindum. Common bunt resistance genes Bt5, Bt6, Bt8, Bt9, Bt10, and Bt11, and several undescribed resistances remain effective in the screening field at ICARDA, Syria.
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  • 31
    ISSN: 1573-5060
    Keywords: Phytophthora clandestina ; resistance ; root rot disease ; subterranean clover ; Trifolium subterraneum ; virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Infection and sporulation of races of Phytophthora clandestina in susceptible and resistant cultivars of subterranean clover and the effect of the races on growth of the cultivars in pots were studied using a total of five races of the pathogen and eight host cultivars. The pathogen caused severe disease where races and cultivars were compatible, although a significant reduction in root and shoot dry weight occurred only on Woogenellup inoculated with race 2. There was little increase in disease severity after 1 wk from the time of inoculation. Sporangia and oospores were formed at high frequency in tap and lateral roots in all compatible combinations, except in Woogenellup in which sporangia were produced inconsistently. The pathogen caused negligible disease and produced very few sporangia in incompatible combinations. Plant age had a strong effect on disease development. Disease severity caused by virulent races on cultivars Gosse, Trikkala, Meteora and Woogenellup was less on older than on younger plants. A further experiment using aggressive isolates of races 0, 1, 2, 3 and 4 revealed that cultivar Gosse showed no reduction in root and shoot weight, despite it being susceptible to races 1, 3 and 4. This provided evidence of the existence of race-non-specific or horizontal resistance to P. clandestina in cultivar Gosse.
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  • 32
    ISSN: 1573-5060
    Keywords: Anthurium ; Radopholus similis ; resistance ; tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five Anthurium species closely related to two of the cultivated ornamentals, A. andraeanum Lind. ex André and A. scherzerianum Schott, and one species of breeding interest, were screened in vitro for tolerance and resistance to the nematode Radopholus similis Cobb, 1893. Adjustment of the tolerance measurement to the initial and uninoculated treatment measurement improved the screening method. Use of a nematode strain with a relatively slow reproduction rate enabled concurrent screening for tolerance and resistance to R. similis in Anthurium. Based on a lower relative symptom index, A. pittieri Engl., A. ravenii Croat and Baker, A. antioquiense Engl. and A. aripoense N. E. Br. reduce nematode damage as compared to the reference standard, ‘Midori’. However, A. antioquiense and A. aripoense had higher root damage than ‘Midori’. Lower nematode damage in A. pittieri and A. ravenii is positively correlated with greater plant vigor or to fewer target roots for nematode infection. A. ravenii was among the most resistant species as measured by nematode reproduction. This is followed by A. aripoense and A. pittieri. Thus, strong plant vigor, fewer target roots for nematode infection, and/or lower nematode reproduction (higher nematode resistance) resulted in lower nematode damage in A. pittieri and A. ravenii. Combining the nematode damage and nematode reproduction results, A. pittieri and A. ravenii were identified as more tolerant than the reference standard ‘Midori’.
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  • 33
    ISSN: 1573-5060
    Keywords: BSA ; Liriomyza trifolii ; Lycopersicon ; molecular markers ; resistance ; leaf miner
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Resistance to many arthropods, including Liriomyza species, is known to be present in accessions of Lycopersicon hirsutum (f. typicum or f. glabratum). From the cross L. esculentum cv. Moneymaker and L. hirsutum f. glabratum G1561 100 F2 plants were screened in a no-choice test for resistance to Liriomyza trifolii. The Bulked Segregant Analysis approach was used to find Random Amplified Polymorphic DNA markers linked to resistance. Two markers were located on chromosome 2. Restriction Fragment Length Polymorphisms constructed a more detailed genetic linkage map for part of chromosome 2. Kruskal-Wallis analysis showed that this chromosome harbored a Quantitative Trait Locus (QTL) for number of pupae, number of mines and damage. At least one major QTL is essential for resistance and this QTL is located on chromosome 2 nearby the location of the tomato probe TG451.
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  • 34
    ISSN: 1573-5060
    Keywords: Hyacinthus ; hyacinth ; Xanthomonas hyacinthi ; resistance ; breeding (pre)selection ; inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Since 1880 ‘yellow disease’ has been known as a very serious disease in hyacinth. The breeding of resistant varieties can constitute an important contribution to the solution of this problem. Therefore it was first aseertained how the differences in resistance between the varieties can be determined as accurately as possible. To this end bulbs and plants of well known cultivars were inoculated with bacteria, and the degree of infection compared with that of bulbs grown in the field. It was found that the infection of the inoculated bulbs corresponded little, if at all, with infection in practice, but the infection of the inoculated leaves did. So the degree of leaf infection seems a useful selection criterion. Comparison of the infection of juvenile and adult plants also yielded an indication of the possibility of preselection for ‘yellow disease’ resistance in seedling populations.
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  • 35
    ISSN: 1573-5060
    Keywords: Dianthus caryophyllus ; carnation ; Phialophora cinerescens ; verticillium wilt ; resistance ; breeding ; diallel analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A breeding programme is described, aimed at introducing resistance to Phialophora cinerescens in glasshouse carnations of both the standard and the spray type. Four resistant clones, selected from seed-grown garden cultivars, served as resistant parents; the susceptible parents included the major glasshouse cultivar William Sim and some of its mutants and a few other American, French and Italian cultivars. In three generations of breeding, with extensive screening of young seedling populations, an adequate level of resistance had been reached. Selection procedure was henceforth changed to allow for primary selection on quality and productivity, followed by clonal resistance tests. Inheritance of resistance appears to be mainly additive; at least two gene pairs are likely to be involved. Under prolonged high infection pressure a significant non-additive variance can be demonstrated.
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  • 36
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    Euphytica 25 (1976), S. 769-775 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Alternaria triticina ; leaf blight ; resistance ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetics of field resistance to Alternaria triticina was studied in a diallel set of crosses using ten cultivars of wheat which included eight resistant and two susceptibles. Susceptibility if NP 830 was found to be controlled by a dominant gene and that of NP 891 by two dominant complementary genes. The resistant cultivars NP 824, NP 835, NP 852, C 281, E 5477, E 5550, E 5878 and UP 303 carry the recessive alleles of the genes present in NP 830 and NP 891. All the resistant cultivars used in the study carried identical gene(s) for resistance to A. triticina. The gene(s) responsible for resistance appear to have come from NP 4 and Turkey, both of which seem to have evolved simultaneously in nature. The gene(s) for susceptibility in NP 830 and NP 891 were found to be different. It is assumed that these genes come from either Motia (Triticum durum) or Khapli (T. dicoccum) in NP 830 and from Gaza (T. durum) in NP 891.
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  • 37
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    Euphytica 27 (1978), S. 75-79 
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; Pyrenochaeta lycopersici ; brown root rot ; corky root ; grey sterile fungus ; resistance ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tomato accessions (Lycopersicon sp.), along with commercial cultivars and breeding lines were grown in a field infested with the brown root rot (BRR) organism, Pyrenochaeta lycopersici and evaluated for resistance. Three L. esculentum Mill. accessions, P.I. 260397, P.I. 262906 and P.I. 203231, were resistant and were used as male parents in crosses designed to transfer resistance to tomatoes of fresh market type. Through analysis of parental generations and F1 and F2 progenies from three crosses the heritability of resistance in the broad sense was estimated to range from 25 to 43 percent. The minimum number of genes influencing resistance was estimated to be from 4 to 8.
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  • 38
    ISSN: 1573-5060
    Keywords: Tulipa ; tulip ; Fusarium oxysporum ; Fusarium bulb rot ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fusarium bulb rot is a serious tulip disease. Breeding for resistance may contribute considerably to a solution of the problem. It has been demonstrated that juvenile and adult bulbs of the same cultivars in Fusarium contaminated soil showed good agreement in degree of resistance. From an incomplete diallel cross of these cultivars second-year bulblets of 62 progenies were planted in both contaminated and non-contaminated soil. The percentages of non-diseased bulbs harvested provided a criterion for resistance. The analysis of combining ability for the degree of resistance revealed that both the mean square of GCA and that of SCA were significant. The relative magnitudes of the GCA and SCA mean squares suggest that resistance is governed primarily by additive gene action. The GCA of individual parents could be estimated and tested. In general it corresponded with their phenotypic behaviour.
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  • 39
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    Euphytica 27 (1978), S. 461-464 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia graminis ; stem rust ; resistance ; inheritance ; monsomic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetics of rust resistance against stem rust race 122 in ‘Chhoti Lerma’ was studied both by conventional and aneuploid analysis. Observations on F1, F2 and F2 backcross progenies revealed the operation of two recessive genes, controlling resistance in ‘Chhoti Lerma’. Monosomic analysis confirmed the operation of two recessive genes conferring resistance to race 122 located on chromosomes 1D and 7D. A minor gene or modifier was also located on chromosome 1B. This was concluded from the fact that F2 of mono's x ‘Chhoti Lerma’ exhibited skewness in favour of resistant plants.
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  • 40
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    Euphytica 36 (1987), S. 571-576 
    ISSN: 1573-5060
    Keywords: Hordeum ; barley ; Rhopalosiphum padi ; brid cherry-oat aphid ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty-seven accessions of Hordeum species and interspecific hybrids were screened in a growth chamber for resistance against the aphid Rhopalosiphum padi. Resistance measurement was based on the aphids' population growth during a 16-day period. The mean number of nymphs produced per female was only about 14% of that on the control cv. Tellus. H. bogdani was the most resistant accession. As a group, interspecific F1-hybrids with common barley (H. vulgare × H. sp.) held an intermediate position. Differences in resistance between hybrids as well as between species (H. jubatum and H. lechleri) were observed. The most resistant species were all diploids. No effects of the host plant on formation of alatae or on the within-plant distribution of aphids were found.
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  • 41
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    Euphytica 36 (1987), S. 719-724 
    ISSN: 1573-5060
    Keywords: Chrysanthemum sp ; chrysant ; Liriomyza trifolii ; leafminer ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In three experiments, Chrysanthemum morifolium Ramat. cultivars and C. pacificum Nakai, were exposed for four days to adult flies of Liriomyza trifolii (Burgess). The flies fed on all plants and laid eggs which were allowed to developed in a leafminer free greenhouse. Feeding level and oviposition varied between genotypes. The largest differences between entries were visible in the percentage of larvae that reached the pupal stage. In the most susceptible C. morifolium cultivar 97% of the larvae pupated against 1% in the most resistant cultivar ‘Penny Lane’. C. pacificum Nakai was also resistant with larval survival rates of 12%, 2% and 0% respectively in three consecutive experiments. Breeding prospects and areas for further study are discussed.
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  • 42
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    Euphytica 34 (1985), S. 309-315 
    ISSN: 1573-5060
    Keywords: Populus spp ; poplar ; Melampsora medusae ; poplar leaf rust ; aggressiveness ; resistance ; differential interaction ; mutation ; virulence ; avirulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Infection Type (IT) and Uredinial Number per Leaf Disk (ULD), induced on eleven cultivars of poplar in vitro, were employed to compare five radiation induced, mono-uredinial mutant lines of Melampsora medusae Thum. with the wild type race 5A from which they were derived. IT produced by the mutants was higher than (eight cultivars), similar to (two cultivars) and pronouncedly less than (IT from 4 to 1 in P. deltoides cv. 7–2) that of race 5A. Although the five mutants produced a uniform IT within a particular cultivar. ULD varied significantly between mutants on individual cultivars and there was a significant differential interaction of the mutant lines with cultivars. The ranking of mutant lines for aggressiveness on cultivars was not consistent while the differences among the mutants in mean ULD over all cultivars were not associated with the dosage level of irradiation from which they were isolated. The implications of the results in the interaction of leaf rust with poplar cultivars are discussed.
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  • 43
    ISSN: 1573-5060
    Keywords: Wild Lactuca species ; Erysiphe cichoracearum ; powdery mildew ; natural infection ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The natural infection of Erysiphe cichoracearum on 29 accessions of five wild Lactuca species (L. serriola, L. saligna. L. virosa, L. aculeata and L. dentata) and on one hybrid of L. serriola x L. sativa has been investigated for two and three years, respectively. No infection was observed on L. serriola (PI 255665), L. saligna (LSA/92/1 and LSA/92/2), L. virosa (LVIR/26 and LVIR/57/1) and L. dentata (PI 234204). The level of susceptibility of the control cultivars of L. sativa was found to be moderate (‘Fila’) to fairly high (‘Hilde’).
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  • 44
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Erysiphe graminis f. sp. hordei ; powdery mildew ; composite cross populations ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Barley powdery mildew was used as a model to evaluate the potential of barley composite cross populations for conservation of disease resistance. The objective was to determine if increases in resistance to powdery mildew could be detected over periods of time in composite cross populations developed in California, where the disease might have had a selective influence on the populations, and the same populations grown in Montana, where no selective influence of powdery mildew was expected. Four isolates of Erysiphe graminis f. sp. hordei were used to monitor the frequencies of plants with specific mildew resistances through early, intermediate and late generations of three composite cross populations (CCII, CCV, CCXII) grown at Davis, California, and Bozeman and Moccasin, Montana. Changes in frequencies of plants resistant to the four isolates were observed between generations in all populations from the three locations. Trends in the frequencies of resistance are discussed in relation to selection pressure applied by E. graminis. It is suggested that associations with gene complexes other than resistance to E. graminis might help to explain the increased resistance observed in these studies. This research was funded in part by U.S. Agency for International Development Contract No. AID/DSAN-C-0024. The authors are grateful to Dr A. L. Kahler for seed of the composite cross populations and to Dr J. G. Moseman for the powdery mildew cultures.
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  • 45
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; L. hirsutum glabratum ; Trialeurodes vaporariorum ; glasshouse whitefly ; resistance ; tomatine ; glycoalkaloids, capillary gas chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The total steroidal alkaloid compositions of the mature-harvested fruits of the tomato cultivar Allround (Lycopersicon esculentum), of the species L. hirsutum glabratum, resistant to the glasshouse whitefly (Trialeurodes vaporariorum), and of four resistant lines were determined by capillary gas chromatography. The contents of α-tomatine in the red-ripe fruits of ‘Allround’ and in those of the lines were low (≤5 mg/kg fresh weight). The green-mature fruits of the wild species had a high content (3390 mg/kg fresh weight) of α-tomatine. The total alkaloid profiles of ‘Allround’ and of the lines were similar. Comparison of flame ionisation detector response with thermoionic detector response indicated that in addition to tomatidine, the aglycone of α-tomatine, a small amount of one other steroidal alkaloid might be present in ‘Allround’ and in the lines. The wild species contained five compounds which possibly are steroidal alkaloids. The safety level of α-tomatine in tomatoes is discussed. It was concluded that, in respect of the glycoalkaloids of the mature-harvested fruits, the resistant tomato breeding lines are as safe for human cosumption as the standard cultivar.
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  • 46
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    Euphytica 36 (1987), S. 609-614 
    ISSN: 1573-5060
    Keywords: Index words (Lactuca spp. ; Microdochium panattoniana ; Marssonina panattoniana ; lettuce anthracnose ; resistance ; specificity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four hundred and forty-nine lines of Lactuca spp. were screened for resistance to an isolate of Microdochium panattoniana. Eighty-seven lines were resistant. Forty-four lines were then screened for resistance to four isolates; twelve groups of lines were identified on the basis of their pattern of resistance. A differential set of lines was used to analyse nine further isolates; this revealed five races of M. panattoniana. Salad Bowl was the only cultivar found to be resistant to three races. A line of L. saligna, UC83US1, was the only line found to be resistant to all the isolates tested.
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  • 47
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    Euphytica 40 (1989), S. 187-191 
    ISSN: 1573-5060
    Keywords: Lycopersicon esculentum ; tomato ; Pyrenochaeta lycopersici ; corky root ; brown root rot ; resistance ; greenhouse screening method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse screening method for corky root (Pyrenochaeta lycopersici) resistance in the tomato (Lycopersicon esculentum) is described in detail. In determining the reliability of the method, known resistance sources within wild asccessions, commerical cultivars and breeding lines were evaluated for resistance in fields naturally infested with corky root and their response compared under greenhouse conditions in soil heavily infested with corky root and artifically maintained at temperatures between 10–13°C. The procedure developed can be used year round and improves the efficiency of recovering resistant progeny in large segregrating populations.
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  • 48
    ISSN: 1573-5060
    Keywords: Dianthus caryophyllus ; carnation ; cultivar ; races ; Fusarium wilt ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A simultaneous analysis of the virulence of races 1, 2 and 4 of Fusarium oxysporum f.sp. dianthi to a series of nine carnation cultivars revealed the presence of different interactions between races and cultivars, as well as differences in pathogenesis between race 1 on the one hand and race 2 and 4 on the other. The most common race 2 induced typical symptoms of Fusarium wilt in all susceptible cultivars. The cultivars showed considerable variation in resistance to race 2. Only Novada remained free of external symptoms throughout the experiment. In diseased plants of all cultivars studied, infected vascular tissue was white with dark brown margins, and heavy degradation of the cell walls and xylem parenchyma cells had occurred. All Dutch isolates corresponded with race 2. Race 4 induced wilt symptoms similar to those induced by race 2, and there was a similar variation in resistance to race 2 and 4 in the cultivars. On average, the race 4 isolates were less aggressive than those of race 2. Compared with race 2, there was evidence of some genotype × race interactions: Pallas proved to be considerably more susceptible, and Lena more resistant to race 4 than to race 2. The isolates of race 4 induced a nistopathology similar to that induced by race 2, but with less vascular browning. Race 1 induced atypical but severe wilt symptoms and unusual vascular discoloration in Elsy, Niky and Sam's Pride only. The vascular tissue in these cultivars turned pale brown; in spite of heavy colonization of these tissues virtually no degradation of cell walls was observed. All other cultivars tested proved virtually resistant to race 1, providing further evidence for genotype × race interactions. Within races, limited but statistically significant genotype × isolate interactions were found as well, in particular within race 4. These are tentatively attributed to independent variation of two (or more) resistance components.
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  • 49
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    Euphytica 42 (1989), S. 25-30 
    ISSN: 1573-5060
    Keywords: Fragaria sp. ; inheritance ; Phytophthora fragariae ; red core ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary It has been found that complete resistance of the strawberry cultivars and selections Earliglow, Guardian, MdUS 2700, MdUS 2929, MdUS 3816 and Redchief to Phytophthora fragariae is inherited in a one-to-one ratio, suggesting that these genotypes have one major resistance gene effective to the races present in the test field. The high level of partial resistance of Cambridge Favourite has clearly been recovered in its progenies. However, from results presented here, no conclusions could be drawn with regard to the number of genes involved in the partial resistance of Cambridge Favourite. It is, however, suggestive that about half the susceptible selections derived from crosses with Cambridge Favourite have shown a relatively high level of partial resistance. The selections concerned were obtained by selection on horticultural characters in seedling populations planted on an uninfested field.
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  • 50
    ISSN: 1573-5060
    Keywords: Cucurbita maxima × C. ecuadorensis ; interspecific cross ; Zucchini yellow mosaic virus ; resistance ; Elisa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inheritance of resistance to zucchini yellow mosaic virus (ZYMV) in the interspecific cross Cucurbita maxima × C. ecuadorensis was studied in generations P1, P2, F1, F2, BCP1 and BCP2. The continuous variation presented in these generations implies a quantitative inheritance. The resistance in C. ecuadorensis was partially dominant over that of sensitivity in C. maxima. Several genes with major effects along with genes with minor effects seem to control ZYMV resistance. Both the dominant and the additive contribution to resistance were found to be significant; their interaction was also significant. However, the major contribution was due to the additive rather than the dominance effects. The narrow sense heritability is 0.91. Virus titer as measured by enzyme linked immunosorbent assay (ELISA) was positively correlated (r=0.76) with the intensity of visual symptoms. The efficiency of ELISA as a tool to the breeders, and the use of C. ecuadorensis as a source of resistance to ZYMV are discussed.
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  • 51
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    Euphytica 42 (1989), S. 233-240 
    ISSN: 1573-5060
    Keywords: Malus sp. ; apple ; Nectria galligena ; apple canker ; resistance ; screening method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To find a fast and reliable test to assess resistance to Nectria galligena in apple, different methods of inoculation were compared using macroconidia of N. galligena and one-year-old cut shoots from mature trees of Cox's Orange Pippin, IVT 69078-19, James Grieve and Jonathan. With the best inoculation method 11 genotypes were screened for resistance. Elstar, Golden Delicious, Jonathan and Lombart's Calville were highly resistant and the level of resistance of Ingrid marie, Gloster, Melrose, IVT 69078-19, Cox's Orange Pippin, James Grieve and Idared decreased in this order. The best inoculation method proved to be simple, giving results within four to nine weeks after inoculation.
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  • 52
    ISSN: 1573-5060
    Keywords: Triticum dicoccoides ; wild emmer wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; minor genes ; temperature-sensitive genes ; resistance ; race-specificity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty-four entries of wild emmer possessing temperature-sensitive genes for resistance to yellow rust were studied in the seedling stage, at two temperature-profiles, with 15 pathogenic races from 11 countries in South America, Africa, Asia and Europe. It was shown that the majority of the resistance genes in these wild emmer entries were race-specific. In most of these entries a more resistant reaction was displayed at the higher temperature-profile; however in three entries a shift in reaction towards resistance was observed with certain races but towards susceptibility with some of the other races, suggesting that two different kinds of temperature-sensitive genes were involved in each of these entries. The similarity of temperature-sensitive genes occurring in wild emmer and in cultivated wheat is discussed.
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  • 53
    ISSN: 1573-5060
    Keywords: Phaseolus vulgaris ; common bean ; Xanthomonas campestris pv. phaseoli ; bacterial disease ; common blight ; rapid leaf chlorosis ; inoculation methods and concentrations ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Common blight disease in beans (Phaseolus vulgaris), caused by the bacterium Xanthomonas campestris pv. phaseoli, reduces crop yield and seed quality. Information is needed on the variation of leaves and pods disease reaction to strains of the bacterium after different inoculation methods. Phaseolus vulgaris cultivars ‘Red Kidney Charlevoix’, ‘GN Harris’, ‘GN 1140’, and ‘GN Emerson’ were inoculated with three different strains of Xanthomonas campestris pv. phaseoli at two inoculum concentrations (108 and 106 bacterial cells/ml) using water soaking, multiple needle, and razor blade inoculation on leaves, and razor blade scratch, dissecting needle, and razor blade cut inoculation on pods. Differential cultivar disease reactions of leaves, pods, or both to the bacterial strains were observed in some cases. Significant interactions among cultivars, inoculation methods, strains, and inoculum concentrations (leaves) were found. A rapid leaf chlorosis developed 6 to 7 days after inoculation. Strains of bacteria did not show specificity in inducing this reaction, but rapid leaf chlorosis was associated with high inoculum concentration and with the water soaking and multiple needle methods. Another experiment was conducted to count the number of living bacterial cells deposited in the leaf tissue after inoculation by different methods. The number of bacteria deposited by water soaking or multiple needle was higher than that deposited by razor blade.
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  • 54
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    Euphytica 44 (1989), S. 61-63 
    ISSN: 1573-5060
    Keywords: Cicer arietinum ; chickpea ; Botrytis cinerea ; grey mould ; inheritance ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Chickpea (Cicer arietinum L.) line ICC 1069 was selected as resistant parent after screening for resistance to grey mould (Botrytis cinerea Pers.) under artificial inoculation conditions. It was crossed with four high yielding susceptible varieties of chickpea. Crosses ICC 1069 × BGM 413 and ICC 1069 × BG 256 showed monogenic dominant resistance in ratio of 3R (resistant): 1S (susceptible). However, in crosses, ICC 1069 × BGM 419 and ICC 1069 × BGM 408, a ratio of 13S (susceptible) : 3R (resistant) was obtained indicating the presence of epistatic interaction. The results pointed towards the presence of a type of major gene resistance to grey mould in chickpea.
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  • 55
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    Euphytica 88 (1996), S. 165-174 
    ISSN: 1573-5060
    Keywords: wheat ; tritordeum ; Hordeum chilense ; Fusarium culmorum ; Septoria nodorum ; scab ; deoxynivalenol ; ergosterol ; resistance ; Triticum spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases.
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  • 56
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    Euphytica 96 (1997), S. 273-279 
    ISSN: 1573-5060
    Keywords: Cicer arietinum ; chickpea ; Fusarium oxysporum f.sp. ciceri ; wilt ; screening ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A total of 1915 Kabuli chickpea lines were screened in a wilt sick plot containing Fusarium oxysporum f.sp. ciceri race 0 at Béja, Tunisia. Complete resistance was found in 110 lines and this result was confirmed by a laboratory screening method. Principal components analysis showed that 〉 80% of the variation of the resistant lines was explained by hundred seed weight and days to maturity. Cluster analysis divided the resistant lines into four groups: 21 had high seed weight (48.25 ± 3.81 g) and early maturity (95.09 ± 2.50 d), 24 had high seed weight (46.84 ± 2.10 g) and late maturity (117.00 d), 34 had low seed weight (22.35 ± 4.72 g) and early maturity (92.97 ± 3.97 d) and 31 had low seed weight (19.62 ± 5.37 g) and late maturity (112.09 ± 4.51 d).
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  • 57
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    Euphytica 97 (1997), S. 39-44 
    ISSN: 1573-5060
    Keywords: Agropyron ; Elymus ; Fusarium head blight ; scab ; resistance ; genetic resources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Four species of indigenous Japanese Agropyron, namely, Elymus humidus Osada (= Agropyron humidum), E. tsukushiensis Honda var. transiens (= A. tsukushiense), E. racemifer Tsvel. (= A. ciliare) and A. mayebaranum var. intermedium Hatusima were collected and evaluated for their resistance to Fusarium head blight (FHB, scab) after inoculation with a conidial suspension of Fusarium graminearum Schwabe at the flowering stage. The resistance to penetration of FHB and to fungal invasion of rachis internodes was evaluated in each accession in a comparison with six wheat cultivars. The results demonstrated that AG.91-35 of E. humidus and AG.91-24 of E. racemifer had higher resistance to penetration than the resistant wheat cultivars Nobeokabouzu-komugi and Sumai 3, respectively. All accessions of indigenous Japanese species of Agropyron examined, with the exception A. mayebaranum, had a statisticallysimilar resistance to invasion, which was the same as that of Nobeokabouzu-komugi or Sumai 3. In all accessions of E. humidus, with only one exception, no spread of the fungus from the infected spikelets to the rachis internodes was detected at all. The possible application of the strains of Agropyron as genetic resources for development of resistance to FHB in wheat is discussed.
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  • 58
    ISSN: 1573-5060
    Keywords: Cajanus Cajan ; inheritance ; isolates ; pigeonpea ; resistance ; sterility mosaic ; susceptibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies were conducted to determine the inheritance of resistance to two isolates of the sterility mosaic pathogen, in three crosses of pigeonpea, involving resistant (ICP 7035, ICP 7349 and ICP 8850) and susceptible (ICP 8863) lines. Observations of F1 and F2 plants were explained on the basis of two independent non-allelic recessive genes for the less virulent, old Patancheru isolate (isolate 1). The backcrosses corroborated the segregation pattern observed in the F2 generation. For the more virulent, new Patancheru isolate (isolate 2), differential behavior of the F1’s was observed. Resistance was dominant in two crosses (ICP 7035 X ICP 8863 and ICP 7349 X ICP 8863), and susceptibility in the other cross (ICP 8850 X ICP 8863). The disease reaction for isolate 2, appeared to be governed by a single gene with three alleles, with one resistance allele exhibiting dominance and the other being recessive, over the allele for susceptibility. Monogenic inheritance of resistance to both isolates was noticed in the cross ICP 8850 X ICP 8863.
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  • 59
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat-alien translocation ; resistance ; C-banding ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wild relatives of common wheat, Triticum aestivum, and related species are an important source of disease and pest resistance and several useful traits have been transferred from these species to wheat. C-banding and in situ hybridization analyses are powerful cytological techniques allowing the detection of alien chromatin in wheat. C-banding permits identification of the wheat and alien chromosomes involved in wheat-alien translocations, whereas genomic in situ hybridization analysis allows determination of their size and breakpoint positions. The present review summarizes the available data on wheat-alien transfers conferring resistance to diseases and pests. Ten of the 57 spontaneous and induced wheat-alien translocations were identified as whole arm translocations with the breakpoints within the centromeric regions. The majority of transfers (45) were identified as terminal translocations with distal alien segments translocated to wheat chromosome arms. Only two intercalary wheat-alien transloctions were identified, one induced by radiation treatment with a small segment of rye chromosome 6RL (H25) inserted into the long arm of wheat chromosome 4A, and the other probably induced by homoeologous recombination with a segment derived from the long arm of a group 7 Agropyron elongatum chromosome with Lr19 inserted into the long arm of 7D. The presented information should be useful for further directed chromosome engineering aimed at producing superior germplasm.
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  • 60
    ISSN: 1573-5060
    Keywords: biotype ; control method ; cowpea ; inheritance ; resistance ; Striga gesnerioides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pot and laboratory experiments were performed in order to elucidate the genetics of resistance of three cowpea (Vigna unguiculata (L.) Walp.) resistant sources to two biotypes of the parasitic weed Striga gesnerioides (Willd.) Vatke (witchweed). B301, Suvita-2 and IT82D-849 were crossed to susceptible cultivars. Roots of each plant of parental, F1, F2, BC1 and BC2 populations were washed free of soil and examined closely for S. gesnerioides attachment. Data on resistant and susceptible cowpea plants were analyzed using the chi-square test to ascertain the goodness of fit to different genetic ratios. Segregation patterns indicate monogenic dominant inheritance of resistance to both S. gesnerioides biotypes in B301. Suvita-2, which is susceptible to the biotype from Maradi, Niger, showed segregation patterns that indicate monogenic dominant inheritance of resistance to S. gesnerioides biotype from Cinzana, Mali. Results also indicate monogenic dominant inheritance of resistance to S. gesnerioides biotype from Cinzana in IT82D-849. However, the inheritance of resistance to the biotype from Maradi would be monogenic and recessive in this line. These results suggest that the resistance of all three sources would be easy to transfer to promising breeding lines.
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  • 61
    ISSN: 1573-5060
    Keywords: cowpea severe mosaic virus ; genetics ; selection ; screening ; resistance ; tolerance ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract P1, P2, F1, F2, BC1 and BC2 generations of four resistant × susceptible crosses and three resistant × resistant crosses of cowpea (Vigna unguiculata L. Walp) were screened for resistance to cowpea severe mosaic virus (CPSMV), in an insect protected screen house. The segregation ratio, at maturity, showed a 63 susceptible : 1 resistant ratio in the F2 generation indicating that resistance is governed by three major genes. The backcross tests and the F3 test confirmed this. The intermediate levels of symptoms observed in the F1 generation and the progression of symptom expression in the F2 generation suggested that resistance is gene dosage dependent. The genetic basis of immunity, tolerance, high level of resistance is discussed based on the three gene model. Delayed symptom expression was evident in genotypes with one or two susceptible alleles. The implications of delayed expression of symptoms in selection and breeding for resistance to CPSMV are discussed.
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  • 62
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    Plant and soil 192 (1997), S. 23-30 
    ISSN: 1573-5036
    Keywords: aluminium ; callose ; protoplasts ; resistance ; toxicity ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The screening of 37 Zea mays L. cultivars in nutrient solution using root elongation (24 h) as a parameter showed large genotypic differences in Al resistance among the genetic material evaluated. Callose concentrations in root tips were closely and positively related to Al-induced inhibition of root elongation. Therefore, Al-induced callose formation in root tips appears to be an excellent indicator of Al injury and can be used as a selection criteria for Al sensitivity. In contrast, aluminium concentrations in root tips were not related to Al-induced inhibition of root elongation, nor to Al-induced callose formation. Callose formation was also induced by short-term A1 treatment in root tip protoplasts, and the response of protoplasts clearly reflected the cultivar-specific response to Al of intact roots. This indicates that in maize, Al sensitivity is expressed on the protoplast level.
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  • 63
    ISSN: 1573-5036
    Keywords: aluminium ; binding forms ; element compartmentation ; Picea abies ; resistance ; root exudates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to investigate if Al resistance in Norway spruce (Picea abies[L.] Karst.) can be attributed to similar exclusion mechanisms as they occur in several crop plants, three-year-old Norway spruce plants were treated for one week in hydroculture with either 500 μM AlCl3 or CaCl2 solutions at pH 4. Sequential root extraction with 1 M NH4Cl and 0.01 M HCl and EDX microanalysis revealed that Al and Ca in cell walls and on the surface participated in exchange processes. About half of the Al extracted by the sequential extraction was not exchangeable by 1 M NH4Cl. Phenolics and phosphate present in the root extracts are possible ligands for Al adsorbed to or precipitated at the root in a non-exchangeable form. In both treatments, C release during the first period of 2 d was much higher than during the remaining time of the experiment. Al treated plants released less total C, carbohydrates and phenolics than did Ca treated plants. Acetate was the only organic acid anion that could be detected in some samples of both treatments. Free amino acids were present at micromolar concentrations but as hydrolysis did not increase their yield, there was no evidence of peptide release. One to two thirds of the released C were large enough not to pass a 1 kDa ultrafilter. The results suggest that exudation of soluble organic complexors is not a major Al tolerance mechanism in Norway spruce, although complexation of Al by phenolic substances released by the root could be detected by fluorescence spectroscopy. Aluminium tolerance could rather be attributed to immobilization in the root apoplast, where strong binding sites are available or precipitation may occur.
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  • 64
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust ; breeding ; resistance ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The most important breeding objectives in crop improvement are improving grain yield, grain quality, and resistances to various biotic and abiotic stresses. The objectives of our study were to compare two crossing and four selection schemes for grain yield, yield traits, and slow rusting resistance to leaf rust (Puccinia recondita) based on additive genes in wheat (Triticum aestivum), and to identify the most efficient crossing and selection methodologies in terms of genetic gains and cost efficiency. Segregating populations were derived from 18 simple crosses and the same number of top (three-way) crosses. Half of the crosses were derived from Yecora 70 and the other half from Veery #10 as the common leaf rust susceptible parents. The four selection schemes were: pedigree, modified bulk (F2 and F1-top as pedigree, selected lines in F3, F4, F2-top, F3-top as bulk; and pedigree in F5 and F4-top populations), selected bulk (selected plants in F2, F3, F4, F1-top, F2-top and F3-top as bulk; and pedigree in F5 and F4-top populations), and nonselected bulk (bulk in F2, F3, F4, F1-top, F2-top and F3-top; and pedigree in F5 and F4-top populations). A total of 320 progeny lines, parents and checks were tested for grain yield, other agronomic traits and leaf rust resistance during the 1992/93 and 1993/94 seasons in Ciudad Obregon (Sonora State, Mexico) which represents a typical high yielding irrigated site. The influence of the type of cross and the selection scheme on the mean grain yield and other traits of the progenies was minimal. The selection of parents was the most important feature in imparting yield potential and other favourable agronomic traits. Moreover, the highest yielding lines were distributed equally. Progeny lines derived from Veery #10 crosses had significantly higher mean grain yield compared to those derived from the Yecora 70 crosses. Furthermore, a large proportion of the highest yielding lines also originated from Veery #10 crosses. Mean leaf rust severity of the top cross progenies was lower than that of the simple cross progenies possibly because two parents contributed resistance to top cross progenies. Mean leaf rust severity of the nonselected bulk derivatives was twice that of lines derived from the other three schemes. Selected bulk appears to be the most attractive selection scheme in terms of genetic gains and cost efficiency.
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  • 65
    ISSN: 1573-5060
    Keywords: diallel analysis ; Fusarium culmorum ; heterosis ; resistance ; scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fusarium head blight (FHB, scab) caused by Fusarium spp. is a widespread disease of cereals causing relevant yield and quality losses and contaminating cereal products with mycotoxins. Breeding resistant cultivars is the method of choice for controlling the disease. Resistance to FHB is a quantitative trait and is most likely governed by several genes. We present the results of an F1 diallel analysis of FHB resistance involving six resistant and one susceptible European winter wheat genotypes of diverse origin in order to identify promising combinations for the selection of improved cultivars. Parents and F1s including reciprocals were evaluated for FHB resistance in an artificially inoculated field trial. Two traits were assessed: visual disease symptoms on the heads and the percentage of Fusarium damaged kernels in a harvested sample. General combining ability (GCA) and specific combining ability (SCA) effects were statistically significant for visual symptoms and kernel damage, whereas reciprocal effects were small or not significant. Heterosis for resistance was common, indicating that the parental genotypes possess different resistance genes. Selection of transgressive segregates should be feasible from such heterotic combinations.
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  • 66
    ISSN: 1573-5060
    Keywords: Phaseolus vulgaris ; common bean ; Phaseolus coccineus ; runner bean ; Colletotrichum lindemuthianum ; anthracnose ; resistance ; physiological races
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The utilization of American and European bean cultivars as host differentials for distinction of races of Colletotrichum lindemuthianum has been discussed. The new race occurring at Ebnet. Germany, since 1973 is named ‘kappa’. It broke down resistance derived from the Are gene originating from Cornell 49–242. Resistance to this kappa race appeared to be present in some European and Asiatic bean cultivars as well as in some American bean accessions.
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  • 67
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    Euphytica 26 (1977), S. 55-62 
    ISSN: 1573-5060
    Keywords: barely ; Hordeum vulgare ; powdery mildew ; Erysiphe graminis f. sp. hordei ; resistance ; resistance genes ; genes for resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten barley mutants and five Ethiopian barley lines representing 11 independently arisen powdery mildew resistance genes in the ml-o locus were tested at the seedling stage to cultures of the powdery mildew fungus from Europe, Israel, USA. Canada, and Japan. They were resistant with infection type 0/(4) in all tests. They were also resistant to field populations of the pathogen when scored in disease nurseries at more than 78 locations in 29 countries in Europe, the Near East, North and South America. New Zealand, and Japan. This indicates that the 11 genes confer the same, world-wide spectrum of powdery mildew resistance. They have no effect on several other barley diseases such as stripe rust and leaf rust.
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  • 68
    ISSN: 1573-5060
    Keywords: Tulipa ; tulip ; Fusarium oxysporum ; resistance ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fusarium oxysporum causes the most serious fungus disease in tulip. Breeding for resistant cultivars may considerably contribute to a solution of the problem. Bulbs of various cultivars planted in contaminated soil showed important and significant differences in resistance. Inoculation trials in the field and in the glasshouse have led to recommendations for the screening of juvenile and adult bulbs.
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  • 69
    ISSN: 1573-5060
    Keywords: Interspecific hybridization ; resistance ; potato leafroll ; virus ; non-tuberous Solanum species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The results of extensive crosses between the non-tuberous species Solanum brevidens and S. etuberosum on the one hand and ten tuber-bearing Solanum species on the other are presented. Three crosses gave rise to viable progeny. Two progenies consisted of diploid plants only of the strictly self-incompatible species of the mother parent. One cross, viz. S. etuberosum × S. pinnatisectum, produced highly vigorous but fully male sterile F1 hybrids. It is suggested that this hybrid together with those between the tomato, Lycopersicon esculentum, and S, pennellii and S. lycopersicoides constitute piers of a bridge between tomato and potato species which in the future might enable gene transfer between these two crops via their wild relatives. However, such idea has to be treated with all proper reserve. The production of this new hybrid is the first step in making accessible to potato breeding the valuable genes which have been detected in S. brevidens and S. etuberosum, viz. the genes for high resistance to frost, leafroll and Y-virus.
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  • 70
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    Euphytica 28 (1979), S. 531-532 
    ISSN: 1573-5060
    Keywords: Linum usitatissimum L. ; linseed ; Oidium lini ; powdery mildew ; resistance ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Resistance to powdery mildew (Oidium lini) in each of the four linseed cultivars, viz., LC 216, LC 255, LC 256 and LC 269 was found to be conditioned by one dominant gene. Crosses between the resistant parents indicated that the same gene was present in all the cultivars and is designated as Ol.
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  • 71
    ISSN: 1573-5060
    Keywords: Nicotiana tabacum ; tobacco ; haploids ; doubled haploids ; tobacco mosaic virus ; potato virus Y ; root knot nematode ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Methods are described for producing large numbers of haploid plantlets from anthers of a flue-cured tobacco hybrid with monogenic resistance to tobacco mosaic virus, (TMV), potato virus Y (PVY) and root knot (RK), respectively. Additional details are given on colchicine treatment for converting haploids to doubled haploids (DH's) and on the frequency of spontaneous DH's among untreated plantlets. Disparate genetic ratios of TMV-resistant to TMV-susceptible plants were obtained among colchicine-treated haploid plantlets, induced DH's and untreated haploids when compared with F2 and BC1 progenies. Haploids (gametes) with the gene for TMV resistance occurred more frequently than expected and plantlets with the gene for RK resistance occurred less frequenctly than expected. Transmission of the gene for PVY resistance differed only slightly from Mendelian expectations. These unexpected ratios, in addition to the frequent occurrence of plastid chimeras among anther-derived plantlets, strengthened our conviction that haploidy is somehow associated with mutation.
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  • 72
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    Euphytica 36 (1987), S. 227-236 
    ISSN: 1573-5060
    Keywords: Vicia faba ; broad bean ; fababean ; Orobanche crenata ; resistance ; root biomass ; shoot biomass ; tubercle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Orobanche attack on twelve broad bean cultivars was measured in connection with vegetative growth by means of two pot tests with different innoculum densities. Orobanche attack appeared to be related to the growth vigour of the host. The higher the root and shoot biomass of a cultivar, the higher the number and the more advanced the development of the tubercles was, independent of innoculum density. Therefore, in spite of the observed variation for Orobanche attack, the tested cultivars were considered to have the same level of susceptibility, however with exception of BPL 2210. This cultivar showed a lower attack than was expected of its growth vigour.
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  • 73
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    Euphytica 34 (1985), S. 425-430 
    ISSN: 1573-5060
    Keywords: Vicia faba ; faba bean ; Ascochyta fabae ; inoculation ; infection conditions ; resistance ; age of leaf
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method was developed for screening faba bean seedlings for resistance to Ascochyta fabae. Several factors were investigated, including amount and concentration of inoculum, period of high humidity and age of leaves. Seedlings of different cultivars were tested and results compared with available field data. Older leaves proved to be less susceptible than younger leaves. Seedling tests reflected differences in resistance in the field, especially in material uniform in growth habit.
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  • 74
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    Euphytica 34 (1985), S. 801-809 
    ISSN: 1573-5060
    Keywords: Ribes ; black currant ; Dasyneura tetensi ; host range ; resistance ; genetics ; gene Dt ; Sphaerotheca mors-uvae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Established Western European black currant cultivars are susceptible to Dasyneura tetensi but the Scandinavian ‘Sunderbyn II’, ‘Kangosfors’ and ‘Hedda’, and 10 out of 33 Russian cultivars showed very slight or no leaf symptoms of midge attack in the field. Of species within the black currant Section Eucoreosma, Ribes dikuscha, R. ‘pauciflorum’ (probably=syn R. ussuriense), and R. americanum were not attacked, but an accession of R. bracteosum proved very susceptible. Species within other sections of the genus were not attacked. A strong dominant resistance gene in R. dikuscha, designated Dt, appeared to be linked with (a) gene(s) controlling resistance to American gooseberry mildew, Sphaerotheca mors-uvae. Resistance in ‘Sunderbyn II’ and, probably, in R. ‘pauciflorum’was oligogenic and dominant.
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  • 75
    ISSN: 1573-5060
    Keywords: Brassica oleracea var. botrytis ; Snowball cauliflower ; Indian cauliflower ; Xanthomonas campestris ; black rot ; resistance ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Black rot disease caused by Xanthomonas campestris pv. campestris is a limiting factor in the commercial production of the cauliflower crop. Crosses were attempted between SN 445, a mid season cultivar resistant to black rot and two highly susceptible commercial cultivars (Pusa Snowball-1 and K-1). Studies of the F1's, F2's and back crosses indicated that SN 445, carries a dominant gene imparting resistance to black rot.
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  • 76
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    Euphytica 35 (1986), S. 993-999 
    ISSN: 1573-5060
    Keywords: Avena ; oats ; Rhopalosiphum padi ; bird cherry-oat aphid ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Thirty-two accessions of Avena species and breeding lines were evaluated for resistance, primarily antibiosis, to the aphid Rhopalosiphum padi (L.) in greenhouse and growth room tests. The highest levels of resistance were found in A. barbata and in the perennial species A. macrostachya. One breeding line, Obee, was also found to have interesting resistance characters. Resistance in A. macrostachya is discussed in relation to perenniality. A different screening method for rapid testing of large collections of varieties is evaluated.
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  • 77
    ISSN: 1573-5060
    Keywords: Raparadish x Brassicoraphanus ; Brassica rapa ; Raphanus sativus ; amphidiploid ; intergeneric hybrid ; fertility ; resistance ; beet cyst nematode ; Heterodera schachtii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Raparadish, x Brassicoraphanus, the amphidiploid hybrid between Brassica rapa (syn. B.campestris) and Raphanus sativus (fodder radish) was made by Dolstra (1982). Primary hybrid plants grew vigorously, suggesting that the amphidiploid AARR might be useful as a fodder crop. Three populations of this new material were studied, with special attention to improvement of fertility and resistance to beet cyst nematode (Heterodera schachtii), whilst preserving genetic variability. For lack of progress one of the populations was abandoned after the fourth generation. The other two populations were observed through nine or ten generations. Apart from the last two generations mass selection for seed set was carried out on the basis of single plants. This led to a considerable increase in average seed production, without losing a wide variation for this trait. Thus more progress is being expected. Five cycles of mass selection for resistance to beet cyst nematodes led to a considerable increase of the level of resistance of both populations. The prospects of this new agricultural crop are discussed.
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  • 78
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    Euphytica 40 (1989), S. 21-29 
    ISSN: 1573-5060
    Keywords: Lactuca sativa ; lettuce ; Macrosiphum euphorbiae ; Myzus persicae ; Nasonovia ribisnigri ; leaf aphids ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three lines of lettuce with resistance to Nasonovia ribisnigri, based on the dominant Nr-gene, and four lines selected for partial resistance to Myzus persicae were tested against three species of leaf aphid: N. ribisnigri, M. persicae and Macrosiphum euphorbiae. The effect of the Nr-gene was also studied in a segregating F2 population. In the material tested, resistance to N. ribisnigri was exclusively based on the Nr-gene, lines selected only for resistance to M. persicae showed no resistance to N. ribisnigri. The Nr-gene also induces partial resistance to M. persicae, but the level of this resistance is influenced by other genes, because the lines with Nr-gene differed significantly from each other for reproduction of M. persicae. The Nr-gene had no effect on the resistance of lettuce to M. euphorbiae. In lines with the Nr-gene, levels of resistance to M. persicae and to M. euphorbiae were correlated, suggesting that the resistance may be determined by the same genes. The Nr-line with highest resistance to M. persicae was comparable for this characteristic to the lines selected for resistance to M. persicae. The cultivars Taiwan and Ravel possess a resistance factor to M. euphorbiae that has no effect on M. persicae or N. ribisnigri. Lines selected for resistance to M. persicae also showed partial resistance to M. euphorbiae. Based on the present results no conclusions can be drawn whether this resistance is based on the same genes that provide resistance to M. persicae, or on a resistance factor comparable to that found in Taiwan and Ravel.
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  • 79
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    Euphytica 40 (1989), S. 103-109 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; triticale ; wheat-triticale hybrids ; Secale cereale ; rye ; Gaeumannomyces graminis ; take-all ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifteen triticale and wheat-triticale hybrid lines were evaluated for resistance to the take-all fungus Gaeumannomyces graminis var. tritici and compared with five wheat and two rye lines in inoculated field and pot trials. The triticale and wheat-triticale hybrid lines varied in rye chromosome number and degree of resistance expressed. One line, Venus with seven pairs of rye chromosomes consistently showed levels of resistance intermediate between wheat and rye. A trend was observed where increasing rye chromosome content led to greater resistance but exceptions showed that variation within triticales could not be ascribed to rye chromosome content alone.
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  • 80
    ISSN: 1573-5060
    Keywords: Glycine max ; soybean ; genetics ; peanut stripe virus ; resistance ; incomplete dominance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inheritance of resistance to a soybean isolate of peanut stripe virus (PStV-strain PN) was studied in three soybean varieties, AGS 129, Ankur, and PI 230971. Genetic analysis was based on necrotic, mosaic and symptomless reactions in inoculated soybeans. A single incompletely dominant gene in AGS 129 was found to confer the resistance to PStV and was tentatively designated as Pst. The homozygous parent AGS 129, possessing the genotype Pst Pst, was immune while Ankur and PI 230971, with a genotype of pst pst, were susceptible showing mosaic symptoms. The heterozygous genotype Pst pst resulting from the cross of either Ankur or PI 230971 with AGS 129 reacted with necrosis, distinctly different from either of the homozygous genotypes. This genotypic effect was confirmed through the phenotypic segregation in BC, F2, and F3.
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  • 81
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    Euphytica 40 (1989), S. 227-232 
    ISSN: 1573-5060
    Keywords: Lactuca ; lettuce ; Bremia lactucae ; resistance ; seedlings
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary More than 200 accessions of three wildLactuca species were screened in the laboratory for race-specific resistance toBremia lactucae. Only a fewLactuca entries showed resistance both as seedlings and in a leaf disc test. Accessions ofL. serriola andL. sativa had a low degree of resistance. The best entries were also compared under field conditions. TheL. saligna entries were totally free from disease in the field test. High resistance was also recorded in a fewL. serriola entries and in lettuce cultivars such as Saffier and Mariska.
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  • 82
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    Euphytica 42 (1989), S. 31-33 
    ISSN: 1573-5060
    Keywords: Capsicum annuum ; garden pepper ; potato virus Y ; resistance
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Bell pepper suffers considerable losses from a strain of potato virus Y (PVYo-sbp). Crosses were attempted between two resistant lines Perennial and S41-1 and two highly susceptible bell pepper commercial cultivars California Wonder and Yolo Wonder. Studies of F1's, F2's, back crosses and F3's indicated that Perennial and S41-1 carry a recessive gene imparting resistance to potato virus Y.
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  • 83
    ISSN: 1573-5060
    Keywords: Lolium perenne ; perennial ryegrass ; L. multiflorum ; Italian ryegrass ; Festuca pratensis ; meadow fescue ; F. arundinacea ; tall fescue ; Festuca × Lolium hybrids ; Phleum pratense ; timothy ; Dactylis glomerata ; cocksfoot ; Meloidogyne naasi ; root-knot nematode ; resistance ; selection
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Reactions of 13 grasses to Meloidogyne naasi varied with species; ryegrasses, fescues and their hybrids were generally susceptible and cocksfoot and timothy resistant. Marked variation in host resistance levels occurred between genotypes within cultivars. Selection of single plants, followed by tests on replicate tillers, identified resistant and susceptible genotypes in both Italian and perennial ryegrass cultivars. Resistant plants had few nematode-induced galls and fewer females and eggs than susceptibles. There was more or less continuous variation, with many genotypes intermediate between extremes of resistance and susceptibility. Selected resistant and susceptible genotypes are of use in assessing variation in nematode populations and as controls for breeding and selection programmes.
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  • 84
    ISSN: 1573-5060
    Keywords: Glucine max ; soybean ; peanut stripe virus ; resistance ; genetics ; linkage
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The possible linkage relationship between hypocotyl color, leaflet shape, resistance to soybean mosaic virus (SMV) and to a soybean isolate of peanut stripe virus (PStV-isolate PN) was examined in two soybean lines AGS 129 and Ankur. Hypocotyl color, leaf shape and reactions to SMV-G1 and PStV were found to be inherited monogenically, with purple hypocotyl color, ovate leaf shape and resistance to both of the viruses being dominant. The reactions to SMV and PStV were conditioned by genes with 9 ± 2.4 percent recombination as coupling phase. They were inherited independently from hypocotyl color and leaf shape.
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  • 85
    ISSN: 1573-5060
    Keywords: Collapse ; cucumis melo ; melon ; resistance ; sudden wilt ; variation
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences in the response of melon genotypes to the sudden wilt disease were observed in several field trials conducted during 1993–1994 in the Arava region of southern Israel. Generally, the disease was more severe in the late summer growing season which is shorter and has higher temperatures than the spring and autumn growing seasons. The Oriental pickling melon breeding line P6a was the most tolerant among the entries tested. The response to the disease was also studied using two segregating families and their progenitors. BSK (tolerant) × P202 (susceptible) and P6a (tolerant) × D17 (susceptible). Wilting percentages of F1, F2 and backcross families were intermediate between the parents, suggesting an additive mode of gene action.
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  • 86
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    Euphytica 96 (1997), S. 327-330 
    ISSN: 1573-5060
    Keywords: amphiploids ; Karnal bunt ; resistance ; Tilletia indica ; tritordeum ; Hordeum chilense
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Reactions of Hordeum chilense accessions H1 and H7 and their amphiploids, HT8, HT9 and HT28 (named as tritordeum) alongwith wheat lines, T22, T24 and T59 used in their synthesis, were studied for resistance to the Karnal bunt pathogen (Tilletia indica) of wheat. Both the accessions of H. chilense and one tritordeum line, HT8, were rated as highly resistant with zero co-efficient of infection, whereas the other two tritordeum lines HT28 and HT9 were rated as moderately susceptible and susceptible with 5.2 and 10.5 co-efficients of infection, respectively, compared to reaction of the wheat lines involved in their synthesis. Karnal bunt infection was maximum on the susceptible wheat cultivar WL-711 with 24.3 co-efficient of infection. All the wheat lines involved in the synthesis of amphiploids were susceptible to Karnal bunt except, T59 (Triticum sphaerococcum) (6X), which showed a moderate level of resistance.
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  • 87
    ISSN: 1573-5060
    Keywords: head scab ; resistance ; Triticeae ; Fusarium graminearum ; ecological conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 1507 accessions from 93 species of 18 genera in Triticeae were screened for resistance to initial infection and resistance to pathogen spread with multi-floret and single-floret injection inoculation methods respectively. The accessions with high resistance were mainly found in perennial genera: Roegneria, Hystrix, Agropyron, Kengyilia and Elymus. Based on differences in resistance, 18 genera screened in Triticeae could be classified into five groups. The species with high resistance were mainly distributed in humid ecological environments.
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  • 88
    ISSN: 1573-5060
    Keywords: Phytophthora clandestina ; resistance ; root rot disease ; subterranean clover ; Trifolium subterraneum ; virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The resistance of 19 cultivars of subterranean clover was screened against 4 races of P. clandestina by mycelial inoculation of roots of 10-day-old seedlings growing in water agar and by growing seedlings in pasteurised potting mix containing infested vermiculite in controlled conditions. The cultivars showed differential resistance (vertical resistance) to races of the pathogen and can be divided into 4 resistance groups. Cultivars Clare, Esperance, Green Range, Junee, Mount Barker, Rosedale, Woogenellup and Yarloop were susceptible to all races. Cultivars Bacchus Marsh, Denmark, Enfield, Gosse, Goulburn, Karridale, Larisa, Leura and Trikkala were susceptible to races 1 and 3, but resistant to races 0 and 2. Cultivar Meteora was susceptible to races 2 and 3, but resistant to races 0 and 1. Cultivar Seaton Park (LF) was resistant to all races. Cultivars also varied in their race-non-specific (horizontal) resistance: cultivars that were susceptible to particular races usually varied in their degree of susceptibility to those races. In particular, Junee was more resistant to all four races than the other cultivars within its group. Similarly, cultivars Gosse, Larisa, Denmark and Trikkala were more resistant to races 1 and 3 than the other cultivars in their group. Races of the pathogen varied in their aggressiveness as well as in their virulence, as shown by the variation in aggressiveness of different isolates of race 0.
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  • 89
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    Euphytica 92 (1996), S. 81-87 
    ISSN: 1573-5060
    Keywords: barley ; net blotch ; Pyrenophora teres ; resistance ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seedlings of a differential barley (Hordeum vulgare L.) series (21 genotypes) and of six check genotypes were used in the greenhouse to assess variation in virulence among 20 single-spore isolates of the net blotch pathogen. Pyrenophora teres Drechs. f.teres Smedeg., collected from various sites in Finland. The experiment was run twice and symptom expression was recorded on the first three leaves. Analysis of second leaf symptom scores from Run 1 indicated differences in virulence between isolates, all of which were pathogenic, and differential resistance among the barleys. The virulence of P. teres isolates appeared to be conditioned by the host barley from which the isolate derived; the average virulence of isolates collected from a susceptible host was greater than that of isolates collected from a less susceptible host. Results from Run 2 were similar regarding resistance within the barleys, but variation in virulence among the P. teres isolates was not consistent with that from Run 1. CI 9819 caries duplicate genes for resistance to P. teres (Rpt1b and Rpt1c), and CI 7548 possesses Rpt3d. Both genotypes were highly resistant to all isolates. The Rpt1a gene of Tifang (CI4407) conferred resistance to all isolates in Run 2, but only to about half of the isolates in Run 1. The checks, including two of the symptomatically most resistant Nordic barley genotypes, were universally susceptible during these stringent tests. No selective pressure has been placed on Finnish isolates of P. teres through previous deployment of major resistance genes, and it is speculated that any variation in virulence among isolates is likely to be due to a combination of evolutionary forces including, natural selection, random genetic drift and gene flow.
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    Euphytica 93 (1997), S. 353-360 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Secale cereale ; powdery mildew ; Erysiphe graminis f.sp. tritici ; resistance ; suppression ; segregation distortion ; wheat ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The location of wheat gene Pm8 for resistance to powdery mildew in the 1RS chromosome segment derived from cereal rye cultivar Petkus was confirmed. There was reduced transmission of the 1BL.1RS chromosome relative to its wheat homologue in four of the five crosses examined. Pm8 was not expressed in some wheat cultivars and selections which possessed the 1RS chromosome identified by the presence of relevant genes for resistance to the three rusts, due to the presence of a dominant suppressor gene(s) in the wheat genome. The frequency of suppression in 1BL.1RS wheats from Mexico was significantly higher than in a group of wheats (both local and exotic) introduced from China and was probably much higher than in European wheats.
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  • 91
    ISSN: 1573-5060
    Keywords: Allium porrum ; autotetraploidy ; introgression ; Phytophthora porri ; resistance ; screening methods ; leek
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new screening method was developed to evaluate resistance of leek (Allium porrum) to Phytophthora porri, based on inoculation by 24 h-immersion of leek plantlets in the 3–6 leaf stage in a suspension of ca. 100 zoospores.ml-1. The immersion test was used for identifying new sources of resistance and to determine the genetic basis of resistance. Within winter leek, five resistance classes were defined on the basis of average field scores of 21 plants. Clones from these plants were tested with the immersion technique. The average scores per resistance class in immersion and field tests were significantly correlated (P〈0.01). The correlation of single-date field scores with the immersion test scores was better in the second half of the epidemic season. A selection experiment yielded a strong response to selection for resistance (53–97%) but no response to selection for susceptibility. This may indicate that resistance is due to few recessive genes in the studied winter leek. Crosses between landraces and winter leek were analyzed by means of F2 (selfed F1) and BC1, progenies. This analysis indicated the presence of few loci with dominant genes for resistance in accession CGN 873243, and additive polygenes in accession Pl 368351.
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  • 92
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    Euphytica 94 (1997), S. 31-36 
    ISSN: 1573-5060
    Keywords: Triticum ; Fusarium graminearum ; resistance ; head scab ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Multi-floret and single-floret injection inoculation methods were used to test 1076 accessions of Triticum for resistance to initial infection and resistance to pathogen spread within spike tissue respectively. The data obtained between years or inoculation dates were comparatively similar and were little influenced by climatic factors, especially humid condition. All the tested materials uniformly exhibited susceptibility to initial infection, but there were great differences in resistance to spread among species or cultivars. The diploid and tetraploid wheats were severely susceptible. Only 30 genotypes of common wheat of T. aestivum concv. vulgare showed high resistance to spread. The highest frequency of high spread resistance existed in these landraces which were distributed in Zhejiang, Hubei, Hunan, Jiangsu provinces and Shanghai City, Guizhou, Sichuan and Yunnan provinces ranked second. The majority of wheat landraces from Hebei, Shanxi, Shaanxi Province and Beijing City showed susceptibility or high susceptibility. No resistance was found in Tibetian wheats.
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  • 93
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    Euphytica 94 (1997), S. 69-73 
    ISSN: 1573-5060
    Keywords: foxtail millet ; herbicide ; resistance ; interspecific hybridization ; sethoxydim ; inheritance ; Setaria italica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Interspecific hybridization between foxtail millet cultivars (Setaria italica) and a green foxtail (S. viridis) resistant to the herbicide sethoxydim were undertaken to breed foxtail millet for improved herbicide resistance. Parents, reciprocal F1 hybrids, F2 selfed derived populations and BC1 backcross progeny were produced and analysed for mortality and fresh weight over a range of dosages. All resistant progeny were 700 times more resistant than susceptible cultivars and was symptom free under current field dosages. Segregations of resistant and susceptible progeny (3:1 in F2 and 1:1 in BC1) were not dependent upon dosage. Heterozygous individuals displayed the same magnitude of resistance as homozygous plants at twelve times the recommended field dosage. Results suggested that sethoxydim resistance in foxtail millet was controlled by a single, completely dominant, nuclear gene.
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  • 94
    ISSN: 1573-5060
    Keywords: Cucumis sativus ; Frankliniella occidentalis ; plant age ; leaf position ; reproduction ; resistance ; cucumber ; western flower thrips
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three greenhouse cucumber (Cucumis sativus L.) accessions, previously selected for low levels of damage after infestation with Frankliniella occidentalis (Pergande), were tested for resistance against F. occidentalis in a no-choice greenhouse experiment at the mature plant stage. The three accessions showed a strong reduction in thrips damage compared to the susceptible control. The effects of leaf position and plant age of the four cucumber accessions on the reproduction of F. occidentalis were determined in a leaf disc assay. Leaf position had a significant effect on thrips reproduction, whereas plant age, within the test range, had not. In general, reproduction was higher on young leaves. Reproduction as measured on leaf discs from certain leaf positions was strongly correlated with resistance of mature plants in the greenhouse, and can therefore be used as a quick test to screen cucumber accessions for resistance against thrips.
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  • 95
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    Genetic resources and crop evolution 43 (1996), S. 569-574 
    ISSN: 1573-5109
    Keywords: bacterial wilt ; germplasm evaluation ; Lycopersicon esculentum ; Pseudomonas solanacearum ; resistance ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seedlings of two hundred and thirty-three accessions of the tomato collection maintained at the Centro Agronómico Tropical de Investigación y Enseñanza, Turrialba, Costa Rica (CATIE) and 7 cultivars used as controls were evaluated for host-plant resistance to 4 virulent strains of Pseudomonas solanacearum representing race 1 biovars 1 and 3. In general, biovar 3 strains wilted seedlings faster than biovar 1 strains but, after 20 days post-inoculation, no significant differences were noted in susceptible control ratings. Significant differences for disease index were noted, but no line with complete resistance was found. For the USA biovar 1 strain UW-25, only 5 accessions, CATIE 17331, 17334, 17349, 17739, 17740, and 2 of the control cultivars, ‘Hawaii 7998’ and ‘UC-82B’ showed some degree of resistance. Conversely, both the frequency and the degree of resistance were high for Costa Rican biovar 1 strain UW-256. For biovar 3, the Costa Rican strain UW-255 was more virulent than the Peruvian strain UW-130. Eight CATIE accessions, 5539, 17331, 17333, 17334, 17345, 17349, 17742, and MIP-CH1, were as resistant as the resistant control ‘Hawaii 7998’ to 3 strains and accession 17740 was as resistant as ‘Hawaii 7998’ to all 4 strains.
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  • 96
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    Genetic resources and crop evolution 43 (1996), S. 79-84 
    ISSN: 1573-5109
    Keywords: Cucurbita pepo ; squash ; genetic resources ; Erysiphe cichoracearum ; cucurbit powdery mildew ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifty-eight cultivars of Cucurbita pepo (mostly zucchini type) were studied in three-year experiments for field resistance to powdery mildew caused by Erysiphe cichoracearum. No genotypes were found with complete resistance to powdery mildew. There were significant differences in the expression of field resistance characterized by some basic epidemiological parameters (maximum infection degree, area below curve). The highest level of field resistance occurred in 13 cultivars including Acceste F1, Albina, ambassador F1, CU-235, Elite F1, Goldfinger, Parmanta F1, Seneca Hybr. F1.
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  • 97
    ISSN: 1573-5109
    Keywords: antibiois ; Mayetiola destructor ; resistance ; Triticum turgidum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hessian fly, Mayetiola destructor (Say), is the major pest of wheat in North Africa. In Morocco, durum wheat (Triticum turgidum L. subsp. durum (Desf). Husn.) losses due to this pest have been estimated at 32%. Genetic resistance is the only economical and practical means of controlling this insect. Field and greenhouse screening of durum wheat genotypes resulted in the identification of one source of resistance to Hessian fly in Morocco. This is the first source of durum wheat Hessian fly-resistance identified in Morocco. This source of resistance expresses a medium level of antibiosis against first-instar Hessian fly larvae; about 25% of the larvae survive on resistant plants. The deployment of varieties that allow for larval survival on resistant plants should reduce selection for biotype development. This source of resistance is being used by CIMMYT/ICARDA and Moroccan breeders to develop resistant durum wheat varieties.
    Type of Medium: Electronic Resource
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  • 98
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 42 (1995), S. 371-372 
    ISSN: 1573-5109
    Keywords: bacterial blight ; interspecific hybridisation ; Matthiola ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Accessions ofM. tricuspidata (L.) R. Br.,M. aspera Boiss. andMatthiola longipetala (Vent.) DC. were tested for their reaction to bacterial blight of stock (M. incana R. Br.), caused byXanthomonas campestris pv.incanae. Potted plants were sprayed with bacterial cell suspension, adjusted to 108 cells/ml. The tested accessions of the three species were found highly resistant, while control plants ofM. incana were severely infected. InterspecificF 1 hybrid plants betweenM. incana andM. tricuspidata also exhibited high resistance to the disease. The implications and the expected difficulties in breeding stock for bacterial blight resistance are discussed.
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  • 99
    ISSN: 1573-5109
    Keywords: Aegilops ; antibiosis ; Mayetiola destructor ; Morocco ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hessian fly, Mayetiola destructor (Say), is the major insect pest of wheat in Morocco. Host plant resistance has been the most effective and practical method of controlling this pest. When 347 accessions of Aegilops species were screened in the greenhouse for resistance to Hessian fly, several accessions of Ae. geniculata Roth, Ae. triuncialis L., Ae. neglecta Req.ex Bertol., Ae. ventricosa Tausch, Ae. cylindrica Host and Ae. markgrafii (Greuter) Hammer showed resistance reaction. All expressed antibiosis as the mechanism of resistance against first instar Hessian fly larvae. These Aegilops sources of resistance could be exploited for transferring Hessian fly resistance to wheat.
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  • 100
    Electronic Resource
    Electronic Resource
    Springer
    Genetic resources and crop evolution 44 (1997), S. 271-275 
    ISSN: 1573-5109
    Keywords: Cucumis spp. ; germplasm evaluation ; wild progenitors ; Pseudomonas syringae pv. lachrymans ; artificial inoculation ; seedlings ; necrotization ; resistance ; gene centers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Under controlled inoculation, a set of 49 accessions belonging to 16 wild species and varieties of the genus Cucumis was tested for resistance to Pseudomonas syringae pv. lachrymans. There was no complete resistance to this pathogen in Cucumis accessions, but limited necrotization of leaves was observed in four accessions (C. africanus (PI 203974), C. anguria var. longipes (CUC 28/1974), C. melo subsp. conomon Baj-Gua (CUM 238/1974), C. zeyheri (PI 299572)). There were some quantitative differences between Cucumis accessions in disease symptom development.
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