ALBERT

Description: Tidal disruption events (TDEs) are transient flares produced when a star is ripped apart by the gravitational field of a supermassive black hole (SMBH). We have observed a transient source in the western nucleus of the merging galaxy pair Arp 299 that radiated 〉1.5 x 10 52 erg at infrared and radio wavelengths but was not luminous at optical or x-ray wavelengths. We interpret this as a TDE with much of its emission reradiated at infrared wavelengths by dust. Efficient reprocessing by dense gas and dust may explain the difference between theoretical predictions and observed luminosities of TDEs. The radio observations resolve an expanding and decelerating jet, probing the jet formation and evolution around a SMBH.

Description: Galaxy mergers and gas accretion from the cosmic web drove the growth of galaxies and their central black holes at early epochs. We report spectroscopic imaging of a multiple merger event in the most luminous known galaxy, WISE J224607.56–052634.9 (W2246–0526), a dust-obscured quasar at redshift 4.6, 1.3 billion years after the Big Bang. Far-infrared dust continuum observations show three galaxy companions around W2246–0526 with disturbed morphologies, connected by streams of dust likely produced by the dynamical interaction. The detection of tidal dusty bridges shows that W2246–0526 is accreting its neighbors, suggesting that merger activity may be a dominant mechanism through which the most luminous galaxies simultaneously obscure and feed their central supermassive black holes.

Description: Compact neutron star binary systems are produced from binary massive stars through stellar evolution involving up to two supernova explosions. The final stages in the formation of these systems have not been directly observed. We report the discovery of iPTF 14gqr (SN 2014ft), a type Ic supernova with a fast-evolving light curve indicating an extremely low ejecta mass (0.2 solar masses) and low kinetic energy (2 x 10 50 ergs). Early photometry and spectroscopy reveal evidence of shock cooling of an extended helium-rich envelope, likely ejected in an intense pre-explosion mass-loss episode of the progenitor. Taken together, we interpret iPTF 14gqr as evidence for ultra-stripped supernovae that form neutron stars in compact binary systems.

Description: The light emitted by all galaxies over the history of the Universe produces the extragalactic background light (EBL) at ultraviolet, optical, and infrared wavelengths. The EBL is a source of opacity for gamma rays via photon-photon interactions, leaving an imprint in the spectra of distant gamma-ray sources. We measured this attenuation using 739 active galaxies and one gamma-ray burst detected by the Fermi Large Area Telescope. This allowed us to reconstruct the evolution of the EBL and determine the star formation history of the Universe over 90% of cosmic time. Our star formation history is consistent with independent measurements from galaxy surveys, peaking at redshift z ~ 2. Upper limits of the EBL at the epoch of reionization suggest a turnover in the abundance of faint galaxies at z ~ 6.

Description: Galaxies grow inefficiently, with only a small percentage of the available gas converted into stars each free-fall time. Feedback processes, such as outflowing winds driven by radiation pressure, supernovae, or supermassive black hole accretion, can act to halt star formation if they heat or expel the gas supply. We report a molecular outflow launched from a dust-rich star-forming galaxy at redshift 5.3, 1 billion years after the Big Bang. The outflow reaches velocities up to 800 kilometers per second relative to the galaxy, is resolved into multiple clumps, and carries mass at a rate within a factor of 2 of the star formation rate. Our results show that molecular outflows can remove a large fraction of the gas available for star formation from galaxies at high redshift.

Description: Schneider et al . (Reports, 5 January 2018, p. 69) used an ad hoc statistical method in their calculation of the stellar initial mass function. Adopting an improved approach, we reanalyze their data and determine a power-law exponent of 2.05–0.13+0.14 . Alternative assumptions regarding dataset completeness and the star formation history model can shift the inferred exponent to 2.11–0.17+0.19 and 2.15–0.13+0.13 , respectively.

Description: Farr and Mandel reanalyze our data, finding initial mass function slopes for high-mass stars in 30 Doradus that agree with our results. However, their reanalysis appears to underpredict the observed number of massive stars. Their technique results in more precise slopes than in our work, strengthening our conclusion that there is an excess of massive stars (〉30 solar masses) in 30 Doradus.

Description: The 30 Doradus star-forming region in the Large Magellanic Cloud is a nearby analog of large star-formation events in the distant universe. We determined the recent formation history and the initial mass function (IMF) of massive stars in 30 Doradus on the basis of spectroscopic observations of 247 stars more massive than 15 solar masses ( M ). The main episode of massive star formation began about 8 million years (My) ago, and the star-formation rate seems to have declined in the last 1 My. The IMF is densely sampled up to 200 M and contains 32 ± 12% more stars above 30 M than predicted by a standard Salpeter IMF. In the mass range of 15 to 200 M , the IMF power-law exponent is 1.90–0.26+0.37 , shallower than the Salpeter value of 2.35.

Description: Spaceflight imposes multiple stresses on biological systems resulting in genome-scale adaptations. Understanding these adaptations and their underlying molecular mechanisms is important to clarifying and reducing the risks associated with spaceflight. One such risk is infection by microbes present in spacecraft and their associated systems and inhabitants. This risk is compounded by results suggesting that some microbes may exhibit increased virulence after exposure to spaceflight conditions. The yeast, S. cerevisiae, is a powerful microbial model system, and its response to spaceflight has been studied for decades. However, to date, these studies have utilized common lab strains. Yet studies on trait variation in S. cerevisiae demonstrate that these lab strains are not representative of wild yeast and instead respond to environmental stimuli in an atypical manner. Thus, it is not clear how transferable these results are to the wild S. cerevisiae strains likely to be encountered during spaceflight. To determine if diverse S. cerevisiae strains exhibit a conserved response to simulated microgravity, we will utilize a collection of 100 S. cerevisiae strains isolated from clinical, environmental and industrial settings. We will place selected S. cerevisiae strains in simulated microgravity using a high-aspect rotating vessel (HARV) and document their transcriptional response by RNA-sequencing and quantify similarities and differences between strains. Our research will have a strong impact on the understanding of how genetic diversity of microorganisms effects their response to spaceflight, and will serve as a platform for further studies.

Description: V350 Sgr is a classical Cepheid suitable for mass determination. It has a hot companion which is prominent in the ultraviolet (UV) and which is not itself a binary. We have obtained two high-resolution echelle spectra of the companion at orbital velocity maximum and minimum with the Space Telescope Imaging Spectrograph on the Hubble Space Telescope in the 1320 to 1510 region. By cross-correlating these spectra we obtained the orbital velocity amplitude of the companion with an uncertainty in the companion amplitude of 1.9 km s(exp 1). This provides a mass ratio of the Cepheid to the companion of 2.1. The UV energy distribution of the companion provides the mass of the companion, yielding a Cepheid mass of 5.2 0.3 solar mass. This mass requires some combination of moderate main sequence core convective overshoot and rotation to match evolutionary tracks.

Description: Alabama is one of the most biodiverse states in the United States and has the greatest diversity of aquatic species. As urbanization continues to increase in Alabama, this biodiversity is at risk. This project partnered with the Land Trust of North Alabama to identify sensitive habitats that are at risk for urbanization within Madison and Limestone counties. The Land Trust of North Alabama works to preserve land, primarily in Madison and Limestone counties of North Alabama, and encourages stewardship through environmental education. The team conducted a supervised classification of land class types utilizing data from Landsat 5 Thematic Mapper (TM), Landsat 8 Operational Land Imager (OLI), and Shuttle Radar Topography Mission Version 4 (SRTM) to identify land cover changes and areas most vulnerable to future urbanization. Through incorporating land classification analysis and additional parameters indicative of urbanization, the team produced an urbanization prediction tool and a landscape fragmentation map. The urban prediction tool identified land highly suitable for development and found that, by 2045, 25% of highly suitable land will be urbanized using the measured 1% growth rate. Ecological impact was established using observation data of species of interest to the project partners. These tools will enable the Land Trust to target high risk areas of land for preservation.

Description: We present 15 high-mass X-ray binary (HMXB) candidates in the disk of M31 for which we are able to infer compact object type, spectral type of the donor star, and age using multiwavelength observations from NuSTAR, Chandra, and the Hubble Space Telescope. The hard X-ray colors and luminosities from NuSTAR permit the tentative classification of accreting X-ray binary systems by compact object type, distinguishing black hole from neutron star systems. We find hard-state black holes, pulsars, and non-magnetized neutron stars associated with optical point-source counterparts with similar frequency. We also find nine non-magnetized neutron stars coincident with globular clusters and an equal number of pulsars with and without point-source optical counterparts. We perform spectral energy distribution (SED) fitting for the most likely optical counterparts to the HMXB candidates, finding seven likely high-mass stars and one possible red helium-burning star. The remaining seven HMXB optical counterparts have poor SED fits, so their companion stars remain unclassified. Using published star formation histories, we find that the majority of HMXB candidatesX-ray sources with UV-bright point-source optical counterpart candidatesare found in regions with star formation bursts less than 50 Myr ago, and three are associated with young stellar ages (〈10 Myr). This is consistent with similar studies of HMXB populations in the Magellanic Clouds, M33, NGC 300, and NGC 2403.

Description: Biocene is the period of new life. When our descendants look back at this period in time, they will see evidence, in the geologic and electronic record, of anthropic climate change, growing population, and scarcity of resources. But they will also see the rebirth of human ingenuity as we overcame the challenges that faced us through nature-inspired exploration. The Periodic Table of Life (PeTaL) is a proposed tool and open source framework that uses artificial intelligence to aid in the systematic inquiry of biology for its application to human systems. This presentation defines the PeTaL concept and workflow. Biomimicry, biophysics, biomimetics, bionics and numerous other terms refer to the use of biology and biological principles to inform practices in other disciplines. For the most part, the domain of inquiry in these fields have been confined to extant biological models with the proponents of biomimicry often citing the evolutionary success of extant organisms relative to extinct ones. The primary objective of this paper is to expand the domain of inquiry for human processes that seek to model those that are, were or could be found in nature with examples that relate to the field of aerospace and to spur development of tools that can work together to accelerate the use of artificial intelligence in problem solving. Specifically specialized fields such as paleomimesis, anthropomimesis and physioteleology are proposed in conjunction with artificial evolution. Blockchain technology may be vital in allowing open source design tools such as PeTaL to democratize design and yet protect intellectual property. The overarching philosophy outlined here can be thought of as physiomimetics, a holistic and systematic way of learning from natural history. The backbone of PeTaL integrates an unstructured database with an ontological model consisting of function, morphology, environment, state of matter and ecosystem. Tools include text classification, thesaurus, data visualization, and analysis. Applications of PeTaL include guiding human space exploration, understanding human and geological history, and discovering new or extinct life.

Description: The OSIRIS-REx spacecraft launched on September 8, 2016, on a seven-year journey to return samples from asteroid (101955) Bennu. This presentation summarizes the scientific results from the Approach and Preliminary Survey phases. Bennu observations are set to begin on August 17, 2018,when the asteroid is bright enough for detection by the PolyCam. PolyCam and MapCam collect data to survey the asteroid environment for any hazards and characterize the asteroid point-source photometric properties. Resolved images acquired during final approach, starting in late October 2018, allow the creation of a shape model using stereophotoclinometry (SPC), needed by both the navigation team and science planners. The OVIRS and OTES spectrometers characterize the point- source spectral properties over a full rotation period, providing a first look at any features and thermophysical properties. TAGSAM is released from the launch container and deployed into the sampling configuration then returned to the stow position.Preliminary Survey follows the Approach Phase in early December 2018. This phase consists of a series of hyperbolic trajectories that cross over the North and South poles and the equator of Bennu at a close-approach distance of 7 km. Images from these Preliminary Survey passes provide data to complete the 75-cm resolution SPC global shape model and solve for the rotation state. Once the shape model is complete, the asteroid coordinate system is defined for co-registration of all data products. These higher-resolution images also constrain the photometric properties and allow for an initial assessment of the geology. In Preliminary Survey the team also obtains the first OLA data, providing a measure of the surface topography. OVIRS and OTES collect data as "ride-along" instruments, with the spacecraft pointing driven by imaging constraints. These data provide a first look at the spectral variation across the surface of Bennu. Radio science measurements, combined with altimetry and imagery, determine Bennu's mass, a prerequisite to placing the spacecraft into orbit in late December 2018. Together, data from the Approach and Preliminary Survey phases set the stage for the extensive mapping planned for 2019. These dates are the baseline plan. Any contingency or unexpected discovery may change this mission profile.

Description: Clouds and hazes are commonplace in the atmospheres of solar system planets and are likely ubiquitous in the atmospheres of extrasolar planets as well. Clouds affect every aspect of a planetary atmosphere, from the transport of radiation, to atmospheric chemistry, to dynamics and they influence - if not control - aspects such as surface temperature and habitability. In my presentations I aim to provide an introduction to the role and properties of clouds in exoplanetary atmospheres and will discuss the lessons learned from the past two decades of studying clouds in brown dwarf atmospheres. I will consider the role clouds play in influencing the spectra of extrasolar giant planets and will discuss the relative simple approaches that have been taken so far to model exoplanet clouds. I will also review how the scattering and extinction efficiencies of cloud particles may be approximated in certain limiting cases of small and large particles in order to facilitate physical understanding and will discuss the need for optimized cloud models that can be applied to exoplanet transmission spectra. Finally I will discuss the various sources of aerosol opacity, including photochemistry, disequilibrium chemistry, and equilibrium condensation.

Description: Meteor showers occur when the Earth encounters a stream of particles liberated from the surface of a comet or, more rarely, an asteroid. Initially, meteoroids follow a trajectory that is similar to that of their parent comet but modified by both the outward flow of gas from the nucleus and radiation pressure. Sublimating gases impart an "ejection velocity" to solid particles in the coma; this ejection velocity is larger for smaller particles but cannot exceed the speed of the gas itself. Radiation pressure provides a repulsive force that, like gravity, follows an inverse square law, and thus effectively reduces the central potential experienced by small particles. Depending on the optical properties of the particle, the speed of the particle may exceed its effective escape velocity; such particles will be unbound and hence excluded from meteoroid streams and meteor showers. These processes also modify the heliocentric distance at which meteoroid orbits cross the ecliptic plane, and can thus move portions of the stream out of range of the Earth. This talk presents recent work on these components of the early evolution of meteoroid streams and their implications for the meteoroid environment seen at Earth.

Description: NASA's Kepler spacecraft, launched in 2009, has been a resounding success. More than 4000 planet candidates have been identified using data from Kepler primary mission, which ended in 2013, and greater than 2000 of these candidates have been verified as bona fide exoplanets. After the loss of two reaction wheels ended the primary mission, the Kepler spacecraft was repurposed in 2014 to observe many fields on the sky for short periods. This new mission, dubbed K2, has led to the discovery of greater than 600 planet candidates, approximately 200 of which have been verified to date; most of these exoplanets are closer to us than the majority of exoplanets discovered by the primary Kepler mission. TESS, launching in 2018, will survey most of the sky for exoplanets, with emphasis on those orbiting nearby and/or bright host stars, making these planets especially well-suited for follow-up observations with other observatories to characterize atmospheric compositions and other properties. More than one-third of the planet candidates found by NASA's are associated with target stars that have more than one planet candidate, and such 'multis' account for the majority of candidates that have been verified as true planets. The large number of multis tells us that flat multiplanet systems like our Solar System are common. Virtually all of the candidate planetary systems are stable, as tested by numerical integrations that assume a physically motivated mass-radius relationship. Statistical studies performed on these candidate systems reveal a great deal about the architecture of planetary systems, including the typical spacing of orbits and flatness. The characteristics of several of the most interesting confirmed Kepler & K2 multi-planet systems will also be discussed.

Description: Humanity is currently on the precipice of a new era: one where human civilization is no longer bound to the confines of a single planet. Now, organizations like the National Aeronautics and Space Administration (NASA) have extended their areas of research and are beginning to focus not just on getting humans into space, but also to keep them safe, healthy, and sane. This focus falls under the Human Research Program (HRP). A focus of this program is "dedicated to discovering the best methods and technologies to support safe, productive human space travel" [1]. For "safe and productive space travel" to occur, astronauts must have proper nutrition [1]. While sending up large amounts of dried and packaged food with every shuttle might work fairly well in the short term, when only a few people need to be fed, it will not be sustainable, especially as NASA looks toward longer space journeys beyond the Earth's orbit. Research into this area falls under Advanced Life Support (ALS), whose mission is to develop regenerative life support systems to support future NASA long-duration missions [2]. This would involve growing crops in space to supplement astronaut diets [2]. An important, yet often overlooked, part of growing crops in any environment is the microbial organisms that inhabit the plants' microbiomes. The Seed Microbiome Project aims to investigate the microbial presence throughout the life stages of three crops, Mizuna Mustard, 'Outredgeous' Red Romaine Lettuce, and 'Red Robin' Tomato, that have either been or will be grown on the ISS.

Description: The International Life Sciences Research Announcement (ILSRA) is a grant which focuses on 'Pick and Eat' salad-crop productivity, nutritional value, and acceptability to supplement the International Space Station (ISS) food system. My contributions into this project were to 1) manage the Veggie chambers to maintain optimal plant growth and attend to any plant needs, 2) analyze data for the VEG-04 Science Verification Test to create a water delivery schedule for the astronauts aboard the ISS, and 3) assume the role as a VEG-04 Experiment Verification Test (EVT) 'pseudonaut' to confirm that all schedules and flight procedures produce quality results. The VEG-04 EVT will continue up until the last day of my internship. Additionally, I'm currently working on two independent research projects, both of which provide insight to potential plant growth hardware options for lunar or Martian surfaces. One of which is a cable culture hydroponics system (favorable in areas with highly limited space), and the other are two aeroponic systems (a subset of hydroponics which uses no media and misting as its delivery for water and nutrients). All independent projects are still being tested and therefore results are not yet established. Lastly, I have been in charge of maintaining the AeroGarden (Registered Trademark), a hydroponic-aeroponic hybrid system which is currently being investigated as a concept to the potential for minimizing human involvement in the process of growing plants. This has the potential to play a major role in future technological project designs for spaceflight hardware. This system is an ongoing project that will continue once my internship ends.

Description: Currently, the goal of plant growth research at KSC (Kennedy Space Center) is to provide a sustainable source of healthy food on long-duration space flights so astronauts and future residents of Mars can get the nutrition they need and produce food, recycle the atmosphere, and aid in recycling water. The sustainable production of food will aid in the efforts of closed life support. Plants have a vital application for bioregenerative life support as demands for food and oxygen can be provided through photosynthesis, while the carbon dioxide from human respiration is removed. In order for a growing medium to provide for plant growth, it needs to provide support, oxygen, ions, and liquid to the plant. This summer research determines how amended Martian regolith simulant acts as a growing medium for plants through looking at the structural properties of each amendment. Mars is covered with regolith which is crushed volcanic rock that is composed of a high amount of perchlorate salts. The composition of Martian soil is such that it is roughly basaltic and consists of sulfur, nitrates, and oxychlorine species. Martian regolith simulants have been generated to further analyze plant growth and aim to replicate features of the reference sample. The JSC (Johnson Space Center) Mars-1A regolith simulant resembles Martian regolith at the Viking I landing site. The objectives of the research this summer at Kennedy Space Center are to devise a plant growth in amended JSC Mars-1A Martian regolith simulant and perform crop studies to assess the performance of full duration crop growth in amended Martian regolith simulant. This research will provide a deeper understanding of how the combination of soil amendments to Martian regolith has a synergistic effect on improving crop production The plant growth experiment will be completed with three treatments each containing inorganic and organic soil amendments.

Description: We demonstrate KLIP forward modeling spectral extraction on Gemini Planet Imager coronagraphic data of HR8799, using PyKLIP. We report new and re-reduced spectrophotometry of HR8799 c, d, and e from H-K bands. We discuss a strategy for choosing optimal KLIP PSF subtraction parameters by injecting fake sources and recovering them over a range of parameters. The K1/K2 spectra for planets c and d are similar to previously published results from the same dataset. We also present a K band spectrum of HR8799e for the first time and show that our H-band spectra agree well with previously published spectra from the VLT/SPHERE instrument. We compare planets c, d, and e with M, L, and T-type field objects. All objects are consistent with low gravity mid-to-late L dwarfs, however, a lack of standard spectra for low gravity late L-type objects lead to poor fit for gravity. We place our results in context of atmospheric models presented in previous publications and discuss differences in the spectra of the three planets.

Description: This release note discusses the science data products produced by the Science Processing Operations Center at Ames Research Center from Sector 1 observations made with the TESS spacecraft and cameras as a means to document instrument performance and data characteristics.

Description: Carbonaceous materials are a primary compo-nent of interstellar dust, forming in the outflow of carbon stars and the diffuse interstellar me-dium (DISM). Over time, the low density DISM is swept into dense molecular clouds, the principal formation sites and repositories of most interstellar molecules. Organic com-pounds created in these clouds are the first step towards the complex materials that help to make planets habitable. Ground- and space-based telescopic observations trace interstellar organics from the diffuse to dense interstellar clouds, revealing that organic material in the diffuse ISM is predominantly hydrocarbon in nature, possessing little N or O, with the C distributed between the aromatic and aliphatic forms. A remarkable similarity between the hydrocarbons in dust in our Galaxy to that of distant galaxies, indicates that this organic component of the DISM is widespread and may be an important universal reservoir of prebiotic organic carbon. Spectroscopy of background stars seen through quiescent dust in clouds with no star formation activity re-veals that chemistry occurs early-on. Mean-while, observations of certain asteroids, com-ets, interplanetary dust particles, planets and planetary satellites present an intricate inter-weaving of preserved interstellar components and those that have been subsequently altered. The composition ofinterstellar dust grains and the evolution of dust between dense clouds (where stars and planetary systems form) and the diffuse inter-stellar medium (where stardust components are ejected), will be presented in this workshop on Carbon in the Solar System.

Description: The Life Sciences Glovebox (LSG) is a rack-level payload facility designed to house biological investigations in a "workbench" type environment aboard the International Space Station (ISS). The facility is scheduled to be launched in September 2018 and will be installed in the Japanese Experiment Module (JEM) of the ISS. LSG is comprised of an extendable work volume, an airlock, an avionics package, a laptop, and supporting structure. The 450L work volume provides two levels of containment for investigations via the physical barrier of its structure and an internal, filtered airflow that results in a negative pressure relative to ISS cabin pressure. The facility provides many other resources for investigation use including 28 VDC power, 120 VAC power, heat rejection, data connections, and video. Portions of the interior surfaces of the work volume are ferrous, allowing investigations and supporting hardware to be magnetically affixed to these surfaces. A UV decontamination system and a variety of ancillary hardware are available for investigations to use while occupying LSG. An engineering unit on the ground is used for payload development and integrated verification testing. Once the facility is installed and commissioned, it will be managed by the same team that manages the Microgravity Science Glovebox (MSG), another rack-level payload facility that has been operating aboard the ISS since 2002. Experienced Investigation Payload Integration Managers (IPIMs), who also support MSG payloads, will be available to assist payload developers through the integration process. This presentation will provide an overview of the LSG facility and the planned investigation flow for the near future.

Description: Organic matter exists in comets (most notably in 81P/Wild 2 [Stardust], 67P/Churyomov- Gerasimenko (67P/C-G) [Rosetta], chondritic porous IDPS, and UCAMMs) and in primitive carbonaceous chondrites that likely retain some chemistry that reflects an origin in the prenatal cold molecular cloud (Alexander+2017). Heavy isotopic enrichments, 15N/14N and possibly D/H, signify preserved molecular cloud organics. In the cold outer disk, if grains are lofted above the disk mid-plane then organics likely experience significant UV processing (Ciesla+2012). In remote sensing of comet comae, organics in the dust are considered refractory or semirefractory. Semi-refractory organics have limited comae lifetimes and produce distributed sources of molecules (H2CO and CO). Rosetta's close passes of 67P/C-G's nucleus (10-15 km) reveals a distributed source of glycine, methyl amine and ethylamine (Altwegg+2016). Cometary samples and primitive meteorites have two types of organic matter: (1) acid-insoluble organic matter (IOM), which is a macromolecular polymer with a mixture of aromatic and aliphatic moieties, and (2) labile, soluble organics, which includes the amino acids, such as glycine (Stardust, Elsila+2009). Meteoritic IOM is robust, withstanding experimental temperatures of 1200 K (Dobrica+2011, Cody+2008). Nanoglobules are a type of IOM; they have a distinct physical structure, but often share the same chemistry as the other IOM from the same meteorite. Moderate-sized PAHs (20 C-atoms) are detected in Stardust samples (Clemett+2010). Refractory organic IOM is ubiquitous yet has a great diversity of abundances between cometary samples. IOM is in primitive chondrites, 67P/C-G (Rosetta), 81P/Wild 2 (Stardust), 1P/Halley, 26P/Grigg-Skjellerup, UCAMMs, anhydrous IDPs, and in chondritic porous IDPs (CP IDPs) and larger cluster IDPs (e.g., Fray+2016, Fomenkova+94, Busemann+ 09, Dobrica+2011, Dobrica+2012). 81P's refractory organic matter is of two types (De Gregorio+2011): nanoglobules of highly aromatic refractory organic matter and polyaromatic carbonyl-containing organic matter, which is similar to IOM in primitive meteorites and IDPs. Fray+2017 estimate that 50% of carbon in 67P/C-G is in IOM. 67P/C-G's organics appear to lack the soluble organic matter, aliphatic carbon, amino acids, and PAHs (Fray+2016). Other notable aspects of the diversity in IOM in cometary samples are the ranges of atomic ratios of N/C, O/C, and H/C, and the range of isotopic enrichments of 15N/14N and D/H. Aqueous and thermal processing on asteroids changes the balance of soluble to insoluble organics, and may be important for diversifying the range of OM delivered to Earth.

Description: In the subalpine zone of the Rocky Mountains, climate change is predicted to result in an increase in the frequency and severity of spruce beetle outbreaks. Climate change itself may affect vegetation, potentially leading to changes in species composition. The direct and indirect effects of climate and disturbances on forest composition, biomass, and dynamics open the possibility for non-linear ecosystem responses. Modeling studies allow for the study of the interaction of these effects and their impact on the forest system. University of Virginia Forest Model Enhanced (UVAFME), an individual-based gap model that simulates forest dynamics and characteristics, is updated with a spruce beetle subroutine that calculates the probability for beetle infestation and potential mortalityof each tree on a plot. The updated model is then run with multiple scenarios that combine beetle infestation with current or altered climate at sites across the southern Rocky Mountains. Results show that spruce beetle infestations acted to facilitate competition with invading lower-elevation species, resulting in an increase in the biomass of historically lower elevation species and a further decline in Engelmann spruce biomass than occurred with solely bark beetle disturbance or solely climate change. We also found an initial enhancing effect between spruce beetle infestation and climate change; however, by the end of 100 yr of climate change and potential beetle infestation, climate had a dampening effect on spruce beetle infestation, through loss of host trees. These results are an important step in understanding the possible futures for vegetation of the Rocky Mountains as well as for spruce forests across the western United States and Canada.

Description: We have successfully flown the EcAMSat (Escherichia coli Antimicrobial Satellite) free-flyer mission. This was a 6U (six unit - CubeSat) small satellite that autonomously conducted an experiment in low Earth orbit to explore the impact of the space environment on antibiotic resistance in uropathogenic E. coli (UPEC) and the role a particular sigma factor plays in the response. After being held in stasis during transport to orbit, two strains - a wildtype UPEC and an isogenic mutant with a deleted gene that encodes a sigma factor - were grown to stationary phase in a fluidic card inside EcAMSat's payload, then incubated with three concentrations of the antibiotic gentamicin. The payload then administered alamarBlue (registered trademark), a redox indicator, into all wells of the fluidic card. The cells were then incubated for 144 hours and metabolic activity was measured optically using the payloads' LED (Light-Emitting Diode) and detector system. Data were then telemetered to the ground and compared to a control experiment conducted in an identical satellite in a lab. The results of this experiment will help us better understand important therapeutic targets for treating bacterial infections on Earth and in space. Such targets are particularly relevant to deep-space and long-duration missions where crew may be more susceptible to infection and treatments for them may work differently.

Description: The universality of water as the solvent for life is usually justified by its role in supporting the rich organic chemistry. It has been pointed out, however, that even richer synthetic chemistry is possible in other organic solvents. Does it mean that water is not necessary for life? Here, other, essential criteria for solvent for life that have not been sufficiently considered are discussed. In biological systems, complex molecules are not only constantly synthesized but also degraded. Solvent-mediated degradation is essential for regulating cell content, preventing overcrowding and allowing for recycling organic material. Achieving a balance between synthetic and degradative processes is facile in water, but not in many other organic liquids. Thus, the so-called water paradox according to which water is both necessary to life and toxic to biopolymer synthesis might not be paradoxical at all. The machinery of life is based on non-covalent interactions that do not involve making or breaking chemical bonds. Their strength needs to be properly tuned. If they are too weak, there might be undesired response to natural fluctuations of physical or chemical parameters. If they are too strong, the kinetics and energetics of cellular processes could adversely influenced. The solvent must allow for balancing these interactions, which provides strong, universal constraints on the medium for life. Water influences non-covalent interactions mainly by two mechanisms. First, it reduces strong, electrostatic interactions between molecules, chemical groups or atoms carrying electric charge or dipole. Second, it induces the hydrophobic effect, the tendency to remove non-polar (hydrophobic) molecules and groups from direct contact with aqueous solution and, instead, interact with each other. In living systems, the hydrophobic effect is largely responsible for self-organization of molecules to more complex structures, such as aggregation of lipid molecules to form biological membranes and protein folding. Water exists as stable liquid in a large temperature range, and the hydrophobic effects are a consequence of the temperature insensitivity of essential properties of its liquid state. In summary, water accomplishes an amazing feat it reduces strong interactions between dissolved species and simultaneously increases the strength of weak interactions, bringing all of them to the right range. Once we consider not only synthetic capabilities but also other required traits of the solvent for life, no viable alternative to water is currently known.

Description: The Alpha Centauri system contains the Solar System's closest stellar neighbors. If an earth-like planet is present in the system, it could in principle be detected using a small space-based telescope. As Alpha Centauri is billions of years old, planets are only expected to be found in regions where their orbits are long-lived. We evaluate the extent of the regions within the Alpha Centauri AB system where small planets are able to orbit for billion-year timescales, as well as how closely-spaced planetary orbits can be within those regions in which individual planets can survive. Individual planets on nearly circular, coplanar orbits can survive throughout the habitable zones of both stars. However, perturbations from the companion star imply that the spacing of such planets in multi-planet systems must be significantly larger than the spacing of similar systems orbiting single stars in order to be long-lived. Because the binary companion induces a forced eccentricity upon the orbits of planets in orbit around either star, appropriately-phased circumstellar orbits with small initial eccentricities are stable to slightly larger initial semi-major axes than are initially circular orbits. Small initial eccentricities have a much larger effect on how closely planetary orbits can be spaced. Our results are of special interest as they can guide observers designing instrumentation and search strategies to attempt to discover planets orbiting the nearest sun-like stars. Results remain significantly higher than for planets orbiting single stars.

Description: NASA's New Horizons mission acquired a large set of images and other data making possible thorough geological analysis of landscapes in the Pluto System. Pluto and Charon exhibit strikingly different surface appearances, despite their similar densities and presumed bulk compositions. Systematic investigation, modelling and mapping revealed that much of Pluto's surface is attributed to surface-atmosphere interactions and the mobilization of volatile ices by insolation. Many mapped valley systems appear to be the consequence of glaciation involving nitrogen ice. Other geological activity requires or required long periods of internal heating, such as Pluto's extensive tectonic fabric. The convection and advection of volatile ices in Sputnik Planitia are thought to be powered by present-day radiogenic heat loss. The prominent mountains at the western margin of Sputnik Planitia, and the strange, multi-km-high mound features to the south, probably composed of H2O, are young geologically as inferred by light cratering and superposition relationships. These multi-km-high mound features might be cryo-volcanoes. Their origin, and what drove their formation so late in Solar System history, is under investigation. East of Sputnik Planitia are large fields of aligned ranks of sharp-crested ridges found only at high altitude that are apparently composed of massive deposits of CH4 ice, referred to as Bladed Terrain. New Horizons found evidence that Bladed Terrain may cover much of Pluto's low latitudes and may have originally formed there as a consequence of Pluto's very high obliquity. Currently Bladed Terrain is undergoing net erosion. This observation, along with evidence for formally more extensive nitrogen glaciation implies that Pluto undergoes significant climate evolution. The dynamic remolding of landscapes by volatile transport seen on Pluto is not unambiguously evident in the mapping of Charon. Charon does, however, display a large resurfaced plain and globally engirdling extensional tectonic network attesting to its early endogenic vigor.

Description: No abstract available

Description: Human behavior often consists of a series of distinct activities, each characterized by a unique pattern of interaction with the visual environment. This is true even in a restricted domain, such as a piloting an aircraft, where activities with distinct visual signatures might be things like communicating, navigating, and monitoring. We propose a novel analysis method for gaze-tracking data, to perform blind discovery of these hypothetical activities. The method is in some respects similar to recurrence analysis, but here we compare not individual fixations, but groups of fixations aggregated over a fixed time interval. The duration of this interval is a parameter that we will refer to as delta. We assume that the environment has been divided into a set of N different areas-of-interest (AOIs). For a given interval of time of duration delta, we compute the proportion of time spent fixating each AOI, resulting in an N-dimensional vector. These proportions can be converted to integer counts by multiplying by delta divided by the average fixation duration (another parameter that we fix at 280 milliseconds). We compare different intervals by computing the chi-square statistic. The p-value associated with the statistic is the likelihood of observing the data under the hypothesis that the data in the two intervals were generated by a single process with a single set of probabilities governing the fixation of each AOI. The method has been applied to approximately 100 hours of eye movement data collected from pilots in a high-fidelity B747 flight simulator, and the results have been compared to synthetic data in which the each activity is represented as first-order Markov process with random probabilities assigned to the AOIs. Randomly-generated synthetic activities can require thousands of fixations to be discriminated with statistical significance, while the human data can be clustered using averaging windows of some 10's of seconds, suggesting that the actual activities are much more narrowly focused than random Markov models.

Description: "NASA's GeneLab Project has evolved from being a simple repository that hosts multi-omics datasets generated from spaceflight experiments, to a complete solution for analysis and visualization of spaceflight related omics. We will show how Omics can help elucidate the impact of spaceflight factors (e.g. CO2) on organisms, tissues and cells."

Description: No abstract available

Description: NASA's BioSentinel mission is one of thirteen secondary payloads to be deployed on the Space Launch System Exploration Mission-1 (SLS EM-1). The BioSentinel nanosatellite will be sent into a heliocentric orbit beyond Low Earth Orbit (LEO), to study the effects of deep space radiation on the budding yeast, Saccharomyces cerevisiae. Ionizing radiation encountered in deep space can create damaging lesions in DNA, including double strand breaks (DSBs). Budding yeast is suitable as a biological model to study these effects, as it is eukaryotic, and can be desiccated for prolonged periods while retaining viability, thus serving as a robust analog for human cells. On the ground, yeast cells are grown in liquid medium, then loaded into the wells of microfluidic cards and air dried prior to integration into the payload. Once the spacecraft reaches its target heliocentric orbit, a mixture of growth medium and metabolic indicator dye will be pumped into the microwells at specific time points to rehydrate the cells and allow them to grow. A 3-color LED detection system will measure changes in growth and metabolism resulting from ionizing radiation exposure. BioSentinel contains a wild type control strain and a rad51 mutant that is defective for DNA damage repair. In this study, we will determine the optimal amount of time to grow diploid yeast cells in liquid culture before they are desiccated for space flight. After an extended time in stationary phase, they become more tolerant to desiccation due to stress caused by nitrogen starvation. However, excessive exposure can lead to loss of viability and to a heterogeneous cell population due to sporulation. Since viability loss during desiccation poses a risk to mission success, a stress preconditioning process during initial growth may increase long-term cell viability. To determine the growth period that improves desiccation tolerance but allows for retention of uniform radiation sensitivity, we will grow both strains in liquid medium for a varying number of days (4 to 7), desiccate the cells, and then observe changes to cell viability and ionizing radiation sensitivity over time. Supported by the Space Life Sciences Training Program at NASA Ames Research Center.

Description: Although there are a large number of known exoplanets, there is little data on their global atmospheric properties.Phase-resolved spectroscopy of transiting planets - continuous spectroscopic observation of planets during their full orbits - probes varied depths and longitudes in the atmospheres thus measuring their three-dimensional thermal and chemical structure and contributing to our understanding of their global circulation. Planets with characteristics suitable for atmospheric characterization have orbits of several days, so phase curve observations are highly resource intensive, especially for shared use facilities. The Exoplanet Climate Infrared TElescope (EXCITE) is a balloon-borne near-infrared spectrometer designed to observe from 1 to 5 meters to perform phase-resolved spectroscopy of hot Jupiters. Flying from a long duration balloon (LDB) platform, EXCITE will have the stability to continuously stare at targets for days at a time and the sensitivity to produce data of the quality and quantity needed to signicantly advance our understanding of exoplanet atmospheres. We describe the EXCITE design and show results of analytic and numerical calculations of the instrument sensitivity. We show that an instrument like EXCITE will produce a wealth of quality data, both complementing and serving as a critical bridge between current and future space-based near infrared spectroscopic instruments.

Description: No abstract available

Description: To search for giant X-ray pulses correlated with the giant radio pulses (GRPs) from the Crab pulsar, we performed a simultaneous observation of the Crab pulsar with the X-ray satellite Hitomi in the 2300 keV band and the Kashima NICT radio telescope in the 1.41.7 GHz band with a net exposure of about 2 ks on 2016 March 25, just before the loss of the Hitomi mission. The timing performance of the Hitomi instruments was confirmed to meet the timing requirement and about 1000 and 100 GRPs were simultaneously observed at the main pulse and inter-pulse phases, respectively, and we found no apparent correlation between the giant radio pulses and the X-ray emission in either the main pulse or inter-pulse phase. All variations are within the 2 fluctuations of the X-ray fluxes at the pulse peaks, and the 3 upper limits of variations of main pulse or inter-pulse GRPs are 22% or 80% of the peak flux in a 0.20 phase width, respectively, in the 2300 keV band. The values for main pulse or inter-pulse GRPs become 25% or 110%, respectively, when the phase width is restricted to the 0.03 phase. Among the upper limits from the Hitomi satellite, those in the 4.510 keV and 70300 keV bands are obtained for the first time, and those in other bands are consistent with previous reports. Numerically, the upper limits of the main pulse and inter-pulse GRPs in the 0.20 phase width are about (2.4 and 9.3) 10(exp 11) erg cm(exp 2), respectively. No significant variability in pulse profiles implies that the GRPs originated from a local place within the magnetosphere. Although the number of photon-emitting particles should temporarily increase to account for the brightening of the radio emission, the results do not statistically rule out variations correlated with the GRPs, because the possible X-ray enhancement may appear due to a 〉0.02% brightening of the pulse-peak flux under such conditions.

Description: No abstract available

Description: Due to advancements in RNA research, mi (micro) RNAs and other small nucleotide RNAs have become a major research field in biology including spaceflight research. The regulation of RNA transcription and processing by miRNAs makes miRNAs an appealing topic for genetics and molecular research. It has been estimated that over 60% of human gene transcripts are targets of miRNA regulation. In fact, this is true for all organisms, including plants and insects. Small nucleotide RNAs can also play a role in regulating gene expression, meaning that gene expression alone is not a complete picture of the potential genetic changes that occur in an organism during spaceflight. The goal of the WetLab-2 project is to isolate and recover miRNAs from various tissue sources on the International Space Station (ISS). No system currently exists that can isolate and recover small nucleotide RNA in space. However, the WetLab-2 system that was validated on the ISS in 2016 can be adapted to fit this purpose. We are currently testing the new modified protocols by running plant and mouse blood experiments in parallel, allowing us to demonstrate the effectiveness of the procedure on different sample types. We expect to be able to optimize and implement the modified miRNA protocols for use on future ISS flights.

Description: Long duration missions to deep space will require new approaches for supplying astronauts. In-space microbial manufacturing could generate many important compounds (such as nutrients, pharmaceuticals and fuels) but there are significant barriers to deploying reliable bioproduction platforms to space. These include ensuring adequate production and proper purification of the desired product, especially in the unique radiation and microgravity environment. Here we are focused on developing methods and technologies to feed microbial factories using the resources available in space. CO2, found in abundance in spacecraft cabins and the Mars atmosphere, can be sequestered and converted into bioproducts. While autotrophic organisms can use CO2 directly, they are generally slow growing and have less-developed biotechnology toolkits. Therefore we are developing an alternative paradigm in which CO2 is first reduced to more energetic carbon compounds that can support more rapid growth of workhorse biotechnology platforms (E. coli, S. cerevisiae, P. pastoris).Various technologies exist or are being developed to convert CO2. For example, the Sabatier system currently installed on the ISS, reacts CO2 and H2 to generate CH4 and H2O. This methane could be consumed by engineered methanotrophic bacteria. Alternatively, electrochemical systems can convert CO2 into formate (CHO2) which could be consumed by formatotrophic bacteria. In either case, synthetic biology techniques allow these microbes to serve as reprogrammable biofactories capable of producing a vast number or products.

Description: The International Space Station (ISS) is a semi-closed habitat in low Earth orbit with environmental conditions provided by an advanced life support system that controls temperature and recycles air and most of the potable water. The crew's activities, such as eating, sleeping, hygiene, and laboratory research, are performed in relatively close proximity. Research in the laboratory includes a myriad of experiments, including those with rodents, plants, and pathogenic microorganisms. Despite these conditions, in-flight monitoring of ISS indicates that the microbial diversity is similar to homes on earth. Accordingly, the crew is generally very healthy, however infectious disease does occur and potential routes of infection by obligate and opportunistic pathogens cannot be completely prevented. Determining the extent of this risk is further complicated, as microorganisms can alter their characteristics in response to spaceflight culture, as exemplified by the increase in virulence of the enteric pathogen Salmonella enterica Typhimurium during spaceflight compared to otherwise identical cultures grown on Earth. Taken together, these factors suggest a need for continued microbiological monitoring and research to understand and mitigate the risk of infectious disease during long duration missions.

Description: Sandy gaps in the shrub matrix of oak (Quercus L.)-saw palmetto (Serenoa repens (W. Bartram) Small) scrub are created by fire but typically close quickly because of rapid regrowth. Such gaps are important habitat features for rare scrub flora and fauna and appear to have been more common in the historical landscape. We followed, from 1993 to 2016, the dynamics of 12 gaps (32.2-98.1 sq m) created by burning slash piles as part of restoration of long-unburned scrub. Gaps closed slowly, primarily by canopy spread of oaks around the gaps. In the absence of subsequent fire, gaps closed within approximately 12 yr. When burned a second or third time, gap area increased to near the initial after-burn size but then declined in area more rapidly than after the initial fire. Vegetation that reestablished in gaps differed from that of the scrub matrix in having less cover of scrub oaks, less cover of S. repens. 〉 0.5 m, greater cover of native shrubs and forbs. 〉 0.5 m, and more bare ground. Soil heating from slash-pile burning killed the roots and rhizomes from which scrub oaks, Serenoa, and ericaceous shrubs sprout; this altered and slowed the after-fire recovery.

Description: Growth of fresh, nutritious, palatable produce for crew consumption during spaceflight may provide health-promoting, bioavailable nutrients and enhance the dietary experience as we move toward longer-duration missions. Tending plants also may serve as a countermeasure for crew psychological stresses associated with long duration spaceflight. However, requirements to support consistent growth of a variety of high quality, nutritious crops under spaceflight environmental conditions is unknown. This study is exploring the potential to grow plants for food production on the International Space Station (ISS) using the Veggie vegetable production system. Ground testing is underway to compare the impacts of several fertilizer and lighting treatments on growth, quality, and nutritional composition of the leafy green crop mizuna, and the dwarf tomato crop "Red Robin" when subjected to Veggie ISS environmental conditions. Early testing focused on the leafy crop "Tokyo Bekana" Chinese cabbage, but ground tests indicated that this plant suffered from stress responses when grown under LEDs and the chronically elevated CO2 levels found on the ISS. Mizuna, a related leafy variety that grows well in the presence of high CO2, and has excellent organoleptic characteristics, was selected as an alternate crop. Tomato crops have been grown using two fertilizer formulations and two pollination techniques, and growth tests using different red:blue lighting environments are underway. Chemical analysis is also being conducted and these data, when coupled with the growth results, will be used to down-select to the two best lighting treatments and best fertilizer treatment for future testing of each crop on the ISS. Additionally, seed-source testing has become important, with mizuna seeds from two different vendors growing very differently. A seed source has been selected, and seed-surface-sanitizing methods have been confirmed for mizuna, but these remain under development for tomato. A crop-handling protocol is also being evaluated to support food safety. All harvests reserve a subset of samples for microbial analysis to determine baseline microbial levels and help establish critical control points for food safety. Testing was initially conducted in hardware analogs of the standard Veggie plant pillows. However, a new Veggie watering system, the Passive Orbital Nutrient Delivery System or PONDS, has been designed and is being prepared for future flight experiments. With the selection of this growth system, ground tests have shifted to analog PONDS systems. Crop tests on ISS, designated VEG-04 for mizuna and VEG-05 for tomato, are planned in 2018 to evaluate any additional impacts of spaceflight on the light and fertilizer conditions down-selected from ground tests. A set of Veggie-specific questions has been developed to characterize the psychological impacts of plant growth and plant-care activities during spaceflight. Organoleptic questionnaires have been developed to assess produce attributes in microgravity taste sessions. These tests for plants growing in the Veggie hardware on ISS will help to mitigate the risk of an inadequate food supply for long duration missions by developing methods and determining hardware requirements to integrate fresh vegetables as a dietary supplement. This research was co-funded by the Human Research Program and Space Biology (MTL#1075) in the ILSRA 2015 NRA call.

Description: The Origins Space Telescope (OST) is studied as a future Mid- and Far-Infrared flagship-class observatory. OST will cover the wavelength range from 6 to 600 microns. To reach the sky background for 200-micron wavelengths temperatures of 4 degrees Kelvin or lower are required. To achieve this low temperature active cooling is required, along with passive shielding and passive radiation to deep space. Currently two concepts are being studied: Concept 1 with a 9-meter-diameter primary and a suite of 5 extremely capable instruments providing both imaging and spectroscopy over the entire wavelength range. Concept 2 is a more modest sized telescope with a collecting area equivalent to a 5-meter primary, fewer deployments and 3 or 4 instruments also covering the entire wavelength range for imaging and spectroscopy, although with somewhat reduced spectroscopic resolution, and somewhat slower mapping speed. This paper will describe OST Concept 2's cryogenic thermal architecture and thermal model results.

Description: An asteroid entering Earth's atmosphere deposits energy along its path due to thermal ablation and dissipative forces that can be measured by ground-based and spaceborne instruments. Inference of pre-entry asteroid properties and characterization of the atmospheric breakup is facilitated by using an analytic fragment-cloud model (FCM) in conjunction with a Genetic Algorithm (GA). This optimization technique is used to inversely solve for the asteroid's entry properties, such as diameter, density, strength, velocity, entry angle, and strength scaling, from simulations using FCM. The previous parameters' fitness evaluation involves minimizing error to ascertain the best match between the physics-based calculated energy deposition and the observed meteors. This steady-state GA provided sets of solutions agreeing with literature, such as the meteor from Chelyabinsk, Russia in 2013 and Tagish Lake, Canada in 2000, which were used as case studies in order to validate the optimization routine. The assisted exploration and exploitation of this multi-dimensional search space enables inference and uncertainty analysis that can inform studies of near-Earth asteroids and consequently improve risk assessment.

Description: Long duration missions will require astronauts to subsist on a closed food system for at least three years. Resupply will not be an option, and the food supply will be older at the time of consumption and more static in variety than previous missions. The space food variety requirements that will both supply nutrition and support continued interest in adequate consumption for a mission of this duration is unknown. Limited food variety of past space programs (Gemini, Apollo, International Space Station) as well as in military operations resulted in monotony, food aversion, and weight loss despite relatively short mission durations of a few days up to several months. In this study, food consumption data from 10 crew members on 3-6-month International Space Station missions was assessed to determine what percentage of the existing food variety was used by crew members, if the food choices correlated to the amount of time in orbit, and whether commonalities in food selections existed across crew members. Complete mission diet logs were recorded on ISS flights from 2008 - 2014, a period in which space food menu variety was consistent, but the food system underwent an extensive reformulation to reduce sodium content. Food consumption data was correlated to the Food on Orbit by Week logs, archived Data Usage Charts, and a food list categorization table using TRIFACTA software and queries in a SQL SERVER 2012 database.

Description: The Wide-Field Infrared Survey Telescope (WFIRST) is planned to have a coronagraphic instrument (CGI) to enable high-contrast direct imaging of exoplanets around nearby stars. The majority of nearby FGK stars are located in multi-star systems, including the Alpha Centauri stars which may represent the best quality targets for the CGI on account of their proximity and brightness potentially allowing the direct imaging of rocky planets. However, a binary system exhibits additional leakage from the off-axis companion star that may be brighter than the target exoplanet. Multi-Star Wavefront Control (MSWC) is a wavefront-control technique that allows suppression of starlight of both stars in a binary system thus enabling direct imaging of circumstellar planets in binary star systems such as Alpha Centauri. We explore the capabilities of the WFIRST CGI instrument to directly image multi-star systems using MSWC. We consider several simulated scenarios using the WFIRST CGI's Shaped Pupil Coronagraph Disk Mask. First, we consider close binaries such as Mu Cassiopeia that require no modifications to the WFIRST CGI instrument and can be implemented as a purely algorithmic solution. Second, we consider wide binaries such as Alpha Centauri that require a diffraction grating to enable suppression of the off-axis starlight leakage at Super-Nyquist separations. We demonstrate via simulation dark holes in 10 percent broadband compatible with the WFIRST CGI.

Description: No abstract available

Description: No abstract available

Description: While it has been shown that decades of astronauts and cosmonauts suffer from immune disorders both during and after spaceflight, the underlying causes are still poorly understood, due in part to the fact that there are so many variables to consider when investigating the human immune system in a complex environment. Invertebrates have become popular models for studying human disease because they are cheap, highly amenable to experimental manipulation, and have innate immune systems with a high genetic similarity to humans. Fruit flies (Drosophila melanogaster) have been shown to experience a dramatic shift in immune gene expression following spaceflight, but are still able to fight off infections when exposed to bacteria. Furthermore, a recent spaceflight mission showed that flies are more susceptible to infection following exposure to microgravity conditions, compared to ground-reared flies from the same population. Additionally, the common bacterial pathogen Serratia marcescens was shown to become more lethal to fruit flies (both space- and ground-reared) after being cultured in space, suggesting that not only do we need to consider host changes in susceptibility, but also changes in the pathogen itself after spaceflight conditions. Being able to simulate spaceflight conditions in a controlled environment on the ground gives us the ability to not only evaluate the effects of microgravity on the host immune system, but also how the microorganisms that cause immune disorders are being affected by these drastic environmental shifts. In this study, I use both spaceflight and ground-based (simulated microgravity) environments to examine the genetic changes associated with increased S. marcescens virulence in order to understand how microgravity is affecting this pathogen, as well as to evaluate how these genetic changes influence and interact with the host immune system. This study will provide us with more directed approaches to studying the effects of spaceflight on human beings, with the ultimate goal of being able to ameliorate human immune dysfunction in future space exploration.

Description: We present a reanalysis of five transit and eight eclipse observations of the ultrashort-period super-Earth 55 Cancri e observed using the Spitzer Space Telescope during 2011-2013. We use pixel-level decorrelation to derive accurate transit and eclipse depths from the Spitzer data, and we perform an extensive error analysis. We focus on determining possible variability in the eclipse data, as was reported in Demory et al. From the transit data, we determine updated orbital parameters, yielding T (sub 0) = 2,455,733.0037 +/- 0.0002, P = 0.7365454 +/- 0.0000003 days, i = 83.5 +/- 1fdg3, and R p = 1.89 +/- 0.05 R . Our transit results are consistent with a constant depth, and we conclude that they are not variable. We find a significant amount of variability between the eight eclipse observations and confirm agreement with Demory et al. through a correlation analysis. We convert the eclipse measurements to brightness temperatures, and generate and discuss several heuristic models that explain the evolution of the planet's eclipse depth versus time. The eclipses are best modeled by a year-to-year variability model, but variability on shorter timescales cannot be ruled out. The derived range of brightness temperatures can be achieved by a dark planet with inefficient heat redistribution intermittently covered over a large fraction of the substellar hemisphere by reflective grains, possibly indicating volcanic activity or cloud variability. This time-variable system should be observable with future space missions, both planned (JWST) and proposed (i.e., ARIEL).

Description: Extended exposure to radiation and microgravity in space has been linked to astronauts developing chronic diseases upon returning to Earth. The Gram-negative pathogen Serratia marcescens has been shown to potentially cause significant infections in humans and in insect models on Earth. Our recent findings also showed that S. marcescens shows an increase in virulence after a short period of growth in the spaceflight environment, which raises initiatives to find the correlation between space environment and the increased virulence. Because we know that the health of astronauts is immunocompromised in space, it is possible that the combination of increased bacterial virulence and the weakened immune system will cause astronauts to be more susceptible to chronic diseases in extended spaceflight. With 75% of human disease genes being conserved in the fruit fly Drosophila melanogaster, these insects act as an ideal model organism to study the human immune system. The high accessibility, low cost, high rate of reproductivity, and short lifespans of D. melanogaster facilitate efficient, high-quality research that seeks to understand altered virulence of this opportunistic pathogen. In this ground-based study, we will use a rotating wall vessel apparatus to simulate microgravity and determine how pathogenicity changes by evaluating differences in gene expression for S. marcescens between bacteria grown in simulated microgravity conditions and controls. We will compare the results of our findings to gene expression patterns in actual spaceflight samples of S. marcescens grown on the ISS (International Space Station) during a recent validation mission, to see if there are common mechanisms across our simulated microgravity and actual spaceflight microgravity samples that both show increased virulence in the fruit fly. With extended space travel in the foreseeable future, understanding how human physiology will be affected by these different factors will help mitigate risks and deaths.

Description: NASA's Kepler Mission was launched in March 2009 as NASA's first mission capable of finding Earth-size planets orbiting in the habitable zone of Sun-like stars, that range of distances for which liquid water would pool on the surface of a rocky planet. Kepler has discovered over 1000 planets and over 4600 candidates, many of them as small as the Earth. Today, Kepler's amazing success seems to be a fait accompli to those unfamiliar with her history. But twenty years ago, there were no planets known outside our solar system, and few people believed it was possible to detect tiny Earth-size planets orbiting other stars. Motivating NASA to select Kepler for launch required a confluence of the right detector technology, advances in signal processing and algorithms, and the power of supercomputing.

Description: No abstract available

Description: A fully automated plant growth facility for conducting plant research supporting space biology and food production projects on the ISS.

Description: The oxygenic photosynthetic bacterium Synechocystis sp. PCC 6803 (S6803) is a model cyanobacterium widely used for fundamental research and biotechnology applications. Due to its polyploidy, existing methods for genome engineering of S6803 require multiple rounds of selection to modify all genome copies, which is time consuming and inefficient. In this study, we engineered the Cas9 tool for onestep, segregationfree genome engineering. We further used our Cas9 tool to delete three of seven S6803 native plasmids. Our results show that all three smallsize native plasmids, but not the largesize native plasmids, can be deleted with this tool. To further facilitate heterologous gene expression in S6803, a shuttle vector based on the native plasmid pCC5.2 was created. The shuttle vector can be introduced into Cas9containing S6803 in one step without requiring segregation and can be stably maintained without antibiotic pressure for at least 30 days. Moreover, genes encoded on the shuttle vector remain functional after 30 days of continuous cultivation without selective pressure. Thus, this study provides a set of new tools for rapid modification of the S6803 genome and for stable expression of heterologous genes, potentially facilitating both fundamental research and biotechnology applications using S6803.

Description: NASA's GeneLab Data System is a repository that hosts multi-omics datasets generated by biological experiments flown onboard the International Space Station. Strategies regarding how GeneLab envisions the involvement of the scientific community and the public at large will be discussed, and current and future capabilities of the system will be described. Information describing how scientists can participate in analyzing the current datasets on plants, microbes, invertebrates or mammals will be provided, and initial findings from the current datasets will be discussed during this presentation. Anyone interested in genomics, transcriptomics, epigenomics and proteomics, and systems biology, or who is curious to understand how space modifies living organisms should attend.

Description: Planetary protection is defined as: a) the prevention of contamination of extraterrestrial bodies by terrestrial microorganisms, and b) biohazard containment of returned samples from bodies in the Solar System that could harbor life.1 While the majority of interplanetary missions to date have involved robotic exploration, future missions will include human explorers. Current planetary protection requirements do not address the unique challenges associated with human exploration. The purpose of this abstract is to review planetary protection efforts for crewed missions and provide a forward plan for implementing them at the systems level. Article IX of the UN Outer Space Treaty of 1967 provides the definition of planetary protection, outlined above.1 COSPAR holds the international standard in line with this treaty2, while NASA's Planetary Protection Policy (NPD 8020.7G) outlines the U.S. implementation of the COSPAR standard. 3;4 NPI 8020.7 groups future human spaceflight planetary protection studies as follows: 1) microbial monitoring, 2) contamination mitigation and control, and 3) environmental effects. Additionally, a NPI 8020.7 outlines a five-step plan for forward work: 1) a literature review, 2) community inputs, 3) completion of recommended studies, 4) developing a draft NPR, and 5) implementation with NASA teams. The literature review was published in 2016.5 Inputs from the community were gathered at the Planetary Protection Knowledge Gaps for Human Extraterrestrial Missions, held in 2015.6 Johnson and Race (2016) outlined notional requirements and prioritized studies needed before final requirements can be produced. This prior work sets the stage for completing the necessary studies and finalizing planetary protection requirements for human spaceflight. We propose a continuation of the systems engineering approach adopted thus far. The challenges associated with the implementation of notional requirements will be quantified in detailed discussions with internal stakeholders. The status and results of high-priority studies that have been completed since 2016 or are ongoing will be incorporated into discussions with stakeholders. In this way, we plan to bridge the gap between the science behind planetary protection and the engineering development that will implement it, allowing finalized planetary protection requirements to be developed for future human space missions.

Description: The use of antimicrobials to control microbiological growth in manned spaceflight water-based systems has and will continue to have a unique set of challenges and needs. The challenges are varied, and include antimicrobial effectiveness, crew health and safety, materials compatibility, optimal system functionality, antimicrobial shelf life, means to monitor antimicrobial concentration, and means to re-introduce biocides periodically in the case of depletion. Needs vary from application to application, and include control of pathogens for crew health, control of biofilm formation for optimal system functionality, inhibition and prevention of microbiologically influenced corrosion, optimization of wetted metallic material life, and general living quarter and consumable aesthetics with respect to odor and taste. This paper outlines and discusses the various antimicrobials used in prior and current manned spaceflight water-based applications with focus on pros, cons and lessons learned. Design factors such as minimum inhibitory concentration, minimum lethal concentration, required circulated concentrations, materials selection, means to introduce, means to monitor real-time, and concentration maintenance are discussed. The challenges associated with longer term missions, as well as long-term system dormancy as envisioned for exploration missions, lunar habitats, and a manned Mars mission are outlined with respect to anticipated needs and potential design solutions.

Description: In support of advanced air revitalization technologies to enable human spaceflight beyond low earth orbit, performance studies have been conducted using a liquid amine, Diglycolamine (DGA) between teams at NASA's Johnson Spaceflight Center (JSC) and Ames Research Center (ARC). Liquid amines have been used in regenerable earth-based systems to remove CO2 from industrial systems as well as for closed-environment air revitalization because they can be regenerated at lower temperatures than solid sorbent systems. As an additional advantage to solid sorbent-based systems, liquid sorbents can be cycled between an adsorbing contactor and degassing chamber, thereby reducing system complexity by operation in a continuous loop. In an effort to inform a regeneration system design for micro-gravity applications, ARC has performed a number of tests to characterize the degas mechanics of DGA. In order to accurately measure the amount of CO2 captured or released by the amine, methods such as gravimetric weighing and chemical desorption are reasonable, however the first iteration test setup for a scaled down degas system required analysis on small sample sizes. Fourier-transform infrared spectroscopy (FTIR) analysis was experimentally evaluated to analyze CO2 concentration because it can produce measurements with sample sizes on the order of 100's of L. Calibration against chemical desorption showed relatively good correlation and test data showed reasonable adherence to expected trends, however more extensive testing should be conducted to fully validate the usage of FTIR to determine CO2 loading on DGA.

Description: Currently no standards or requirements exist for microbial food safety for space grown produce (fresh plant foods). Without standards it is difficult to assess produce handling and sanitization options for the ISS and future exploration missions. We are conducting a literature review of microbial levels on fresh food and then carrying out measurements (microbial counts) of grocery store purchased and controlled environment-grown crops. Testing will include lettuce, mizuna, cherry tomato, pepper, and radish, all candidate crops for pick-and-eat testing on ISS and near term exploration missions. Growth chamber conditions will be set to mimic an ISS or spacecraft environment. Assays will include specific pathogens (Enterobacteriacea, Salmonella sp., and Aspergillus flavus) and total culturable microorganisms using aerobic plate counts, and total yeast and mold counts. Analyses will follow the FDA Bacteriological Analytical Manual methods. The goal of the project is to establish a baseline for expected microbial levels found on fresh plant foods that might be grown on ISS and near term missions, and develop risk assessment and microbial safety recommendations for these types of fresh foods.

Description: A newly discovered assemblage of predominantly small tracks from the Cretaceous Patuxent Formation at NASAs Goddard Space Flight Center, Maryland, reveals one of the highest track densities and diversities ever reported (~70 tracks, representing at least eight morphotypes from an area of only ~2m(exp 2)). The assemblage is dominated by small mammal tracks including the new ichnotxon Sederipes goddardensis, indicating sitting postures. Small crow-sized theropod trackways, the first from this unit, indicate social trackmakers and suggest slow-paced foraging behavior. Tracks of pterosaurs, and other small vertebrates suggest activity on an organic-rich substrate. Large well-preserved sauropod and nodosaurs tracks indicate the presence of large dinosaurs. The Patuxent Formation together with the recently reported Angolan assemblage comprise the worlds two largest Mesozoic mammal footprint assemblages. The high density of footprint registration at the NASA site indicates special preservational and taphonomic conditions. These include early, penecontemporaneous deposition of siderite in organic rich, reducing wetland settings where even the flesh of body fossils can be mummified. Thus, the track-rich ironstone substrates of the Patuxent Formation, appear to preserve a unique vertebrate ichnofacies, with associated, exceptionally-preserved body fossil remains for which there are currently no other similar examples preserved in the fossil record.

Description: No abstract available

Description: Florida scrub-jays are a species listed under the Endangered Species Act. The NASA Ecology program has been a partner for conservation, recovery, and translocation across the species range. The objectives of this talk are to update members of the Archie Carr Working Group recovery, conservation, and translocation activities and describe how the Archie Carr National Wildlife Refuge and nearby conservation lands relate to species recovery actions.

Description: Spaceflight imposes multiple stresses on biological systems resulting in genome-scale adaptations. Understanding these adaptations and their underlying molecular mechanisms is important to clarifying and reducing the risks associated with spaceflight. One such risk is infection by microbes present in spacecraft and their associated systems and inhabitants. This risk is compounded by results suggesting that some microbes may exhibit increased virulence after exposure to spaceflight conditions. The yeast, S. cerevisiae, is a powerful microbial model system, and it's response to spaceflight has been studied for decades. However, to date, these studies have utilized common lab strains. Yet studies on trait variation in S. cerevisiae demonstrate that these lab strains are not representative of wild yeast and instead respond to environmental stimuli in an a typical manner. Thus, it is not clear how transferable these results are to the wild S. cerevisiae strains likely to be encountered during spaceflight. To determine if diverse S. cerevisiae strains exhibit a conserved response to simulated microgravity, we will utilize a collection of 100 S. cerevisiae strains isolated from clinical, environmental and industrial settings. We will place selected S. cerevisiae strains in simulated microgravity using a high-aspect rotating vessel (HARV) and document their transcriptional response by RNA-sequencing and quantify similarities and differences between strains. Our research will have a strong impact on the understanding of how genetic diversity of microorganisms effects their response to spaceflight, and will serve as a platform for further studies.

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Description: In space, astronauts are more susceptible to pathogens, viral reactivation and immunosuppression, which poses limits to their health and the mission. Interestingly, during space flight, stress-inducible heat shock proteins (HSP) are robustly induced, and the overexpression of HSPs have been implicated in immune dysregulation, therefore HSPs may be critically involved in regulating immune homeostasis. HSP40/DNAJ1 plays a major role in proper protein translation and folding. Its loss of function has been implicated in susceptibility to microbial infection, while its overexpression has been implicated in autoimmunity, collectively suggesting its complicated, but necessary, role in maintaining immunological function. To determine the role of HSP40 during stress-induced altered gravity conditions, wild-type and Hsp40 mutant Drosophila melanogaster were exposed to ground-based chronic hypergravity conditions, followed by quantitative PCR (qPCR) analysis of immune gene expression. In addition, larval hemocytes were collected to determine the functional output in response to E. coli bioparticle phagocytosis. Preliminary data indicates a required role for Hsp40 in strengthening immune function during stress-induced spaceflight in flies. In short, a critical need to evaluate the relationship between HSPs and immune suppression during space flight is necessary. Since space travel may become available to the general public in the not too distant future, and for the possibility of long-term space missions, a more comprehensive evaluation of the molecules responsible for immune dysfunction observed during space flight is required.

Description: We obtained high-resolution (lambda divided by delta times lambda equals approximately 25,000) pre-perihelion spectra of Comet C/2013 V5 (Oukaimeden) using NIRSPEC at Keck II on UT 2014 September 5-6, and CSHELL (Cryogenic Echelle Spectrograph) at the NASA-Infrared Telescope Facility on September 11-13, altogether spanning a range in heliocentric distance R (sub h) equals 0.789-0.698 astronomical units. We report water production rates, and production rates and abundance ratios relative to co-measured H2O for eight trace molecules: CO, H2CO, CH3OH, CH4, C2H2, C2H6, HCN, and NH3. Our measured water production rates from NIRSPEC ( Near Infra-Red Spectrograph) and CSHELL observations remained relatively constant and were close to those from SOHO (Solar and Heliospheric Observatory) / Solar Wind Anisotropies observations that encompassed our dates, suggesting H2O production dominated by release directly from or within approximately 2000 kilometers of the nucleus. All trace volatiles were depleted relative to their respective median abundances among comets, excepting NH3, which was consistent with its median abundance. Most surprising were pronounced increases in abundance ratios for CH3OH (by 51 percent relative to simultaneously measured H2O) and especially C2H6 (by 87 percent) between September 5 and 6. On September 5, C2H6 was severely depleted, consistent with its lowest abundance yet measured for any comet. It also tracked the spatial profile of H2O, suggesting C2H6 was associated with a polar ice phase dominating gas production. On September 6, C2H6 was only moderately depleted and was spatially distinct from H2O, suggesting both polar- and nonpolar-dominated ice phases contributed to the activity then. Our results are consistent with a nonhomogeneous volatile composition for C/2013V5, implying differential processing of its constituent ices.

Description: Sulfur has been observed to be severely depleted in dense clouds leading to uncertainty in the molecules that contain it and the chemistry behind their evolution. Here, we aim to shed light on the sulfur chemistry in young stellar objects (YSOs) by using high-resolution infrared spectroscopy of absorption by the 3 rovibrational band of SO2 obtained with the Echelon-Cross-Echelle Spectrograph on the Stratospheric Observatory for Infrared Astronomy. Using local thermodynamic equilibrium models we derive physical parameters for the SO2 gas in the massive YSO MonR2 IRS3. This yields a SO2/H abundance lower limit of 5.6 0.5 10(exp -7), or 〉4% of the cosmic sulfur budget, and an intrinsic line width (Doppler parameter) of b 〈 3.20 km s(exp -1). The small line widths and high temperature (Tex = 234 15 K) locate the gas in a relatively quiescent region near the YSO, presumably in the hot core where ices have evaporated. This sublimation unlocks a volatile sulfur reservoir (e.g., sulfur allotropes as detected abundantly in comet 67P/ChuryumovGerasimenko), which is followed by SO2 formation by warm, dense gas-phase chemistry. The narrowness of the lines makes formation of SO2 from sulfur sputtered off grains in shocks less likely toward MonR2 IRS3.

Description: We present simultaneous optical and radar observations of meteors observed over the Arecibo radar in Puerto Rico. These observations were obtained during ~24 h of radar time over 3 different nights in May 2012. The radar was operating in a dual-frequency mode at 430 MHz (UHF) and 46.8 MHz (VHF). The optical observations were made at 41 frames per second with an Andor Electron Multiplying CCD camera with a field of view of ~6, enabling the determination of accurate horizontal meteor velocities and optical light curves. There are varying detection sensitivities of the 3 different techniques (2 radar, 1 optical). The UHF radar is the most sensitive, resulting in a total of 8710 detected meteors during that period, while the VHF radar is significantly less sensitive resulting in only 278 detected meteors. The optical observations are the least sensitive, resulting in a total of 178 detections. Of all of these meteors, only 19 were simultaneously detected by the 3 instruments. This paper focuses on the analysis of these 19 common events. The meteoroid masses determined from the optical light curves were found to be in the range of 20 g to 1.2 mg and did not correlate with the maximum Signal-to-Noise Ratio (SNR) of either radar. In addition, the UHF radar detected 5 out of these 19 meteors with far side-lobes, beyond the main-beam and first side-lobe. This indicates that a significant fraction of events detected by the UHF radar could be in far side lobes and therefore have significantly underestimated Radar Cross Sections (RCS), given the smaller backscattered powers received in the side lobes.

Description: This payload overview presentation will be presented at the Payload Operations Integration Working Group (POIWG) on October 25th, 2018. It provides a high-level overview of Cell Science-03 operations.

Description: The Imaging X-ray Polarimetry Explorer (IXPE) will expand the information space for study of cosmic sources, by adding linear polarization to the properties (time, energy, and position) observed in x-ray astronomy. Selected in 2017 January as a NASA Astrophysics Small Explorer (SMEX) mission, IXPE will be launched into an equatorial orbit in 2021. The IXPE mission will provide scientifically meaningful measurements of the x-ray polarization of a few dozen sources in the 2-8 keV band, including polarization maps of several x-ray-bright extended sources and phase-resolved polarimetry of many bright pulsating x-ray sources.

Description: Future NASA concept missions that are currently under study, like the Habitable Exoplanet Imaging Mission (HabEx) and the Large Ultra-violet Optical Infra Red Surveyor (UVOIR), could discover a large diversity of exoplanets. We propose here a classification scheme that distinguishes exoplanets into different categories based on their size and incident stellar flux, for the purpose of providing the expected number of exoplanets observed (yield) with direct imaging missions. The boundaries of this classification can be computed using the known chemical behavior of gases and condensates at different pressures and temperatures in a planetary atmosphere. In this study, we initially focus on condensation curves for sphalerite ZnS, H2O, CO2, and CH4. The order in which these species condense in a planetary atmosphere define the boundaries between different classes of planets. Broadly, the planets are divided into rocky planets (0.5-1.0 solar radius), super-Earths (1.0-1.75 solar radius), sub-Neptunes (1.75-3.5 solar radius), sub-Jovians (3.5-6.0 solar radius), and Jovians (6-14.3 solar radius) based on their planet sizes, and "hot," "warm," and "cold" based on the incident stellar flux. We then calculate planet occurrence rates within these boundaries for different kinds of exoplanets, eta (sub planet) [i.e. exoplanet], using the community coordinated results of NASA's Exoplanet Program Analysis Group's Science Analysis Group-13 (SAG-13). These occurrence rate estimates are in turn used to estimate the expected exoplanet yields for direct imaging missions of different telescope diameters.

Description: The Gaia catalogue second data release and its implications to optical observations of man-made Earth orbiting objects. Abstract and not the Final Paper is attached. The Gaia spacecraft was launched in December 2013 by the European Space Agency to produce a three-dimensional, dynamic map of objects within the Milky Way. Gaia's first year of data was released in September 2016. Common sources from the first data release have been combined with the Tycho-2 catalogue to provide a 5 parameter astrometric solution for approximately 2 million stars. The second Gaia data release is scheduled to come out in April 2018 and is expected to provide astrometry and photometry for more than 1 billion stars, a subset of which with a the full 6 parameter astrometric solution (adding radial velocity) and positional accuracy better than 0.002 arcsec (2 mas). In addition to precise astrometry, a unique opportunity exists with the Gaia catalogue in its production of accurate, broadband photometry using the Gaia G filter. In the past, clear filters have been used by various groups to maximize likelihood of detection of dim man-made objects but these data were very difficult to calibrate. With the second release of the Gaia catalogue, a ground based system utilizing the G band filter will have access to 1.5 billion all-sky calibration sources down to an accuracy of 0.02 magnitudes or better. In this talk, we will discuss the advantages and practicalities of implementing the Gaia filters and catalogue into data pipelines designed for optical observations of man-made objects.

Description: No abstract available

Description: GeneLab as a general tool for the scientific community; Utilizing GeneLab datasets to generate hypothesis and determining potential biological targets against health risks due to long-term space missions; How can OpenTarget be used to discover novel drugs to test as countermeasures that can be utilized by astronauts.

Description: NASA has flown animals to space as part of trailblazing missions and to understand the biological responses to spaceflight. Mice traveled in the Lunar Module with the Apollo 17 astronauts and now mice are frequent research subjects in LEO on the ISS. The ISS rodent missions have focused on unravelling biological mechanisms, better understanding risks to astronaut health, and testing candidate countermeasures. A critical barrier for longer-duration animal missions is the need for humans-in-the-loop to perform animal husbandry and perform routine tasks during a mission. Using autonomous or telerobotic systems to alleviate some of these tasks would enable longer-duration missions to be performed at the Deep Space Gateway. Rodent missions performed using the Gateway as a platform could address a number of critical risks identified by the Human Research Program (HRP), as well as Space Biology Program questions identified by NRC Decadal Survey on Biological and Physical Sciences in Space, (2011). HRP risk areas of potentially greatest relevance that the Gateway rodent missions can address include those related to visual impairment (VIIP) and radiation risks to central nervous system, cardiovascular disease, as well as countermeasure testing. Space Biology focus areas addressed by the Gateway rodent missions include mechanisms and combinatorial effects of microgravity and radiation. The objectives of the work proposed here are to 1) develop capability for semi-autonomous rodent research in cis-lunar orbit, 2) conduct key experiments for testing countermeasures against low gravity and space radiation. The hardware and operations system developed will enable experiments at least one month in duration, which potentially could be extended to one year in duration. To gain novel insights into the health risks to crew of deep space travel (i.e., exposure to space radiation), results obtained from Gateway flight rodents can be compared to ground control groups and separate groups of mice exposed to simulated Galactic Cosmic Radiation (at the NASA Space Radiation Lab). Results can then be compared to identical experiments conducted on the ISS. Together results from Gateway, ground-based, and ISS rodent experiments will provide novel insight into the effects of space radiation.

Description: No abstract available

Description: Clinical exposure to ionizing radiation could put cancer radiotherapy or bone allograft patients at an increased risk of fracture. In these applications, ionizing radiation levels can range from accumulative 50 Gy for radiotherapy cancer treatment, to acute 35,000 Gy for allograft sterilization. Ionizing radiation has been shown to decrease bon equality through reduced strength and post-yield properties and degrade collagen integrity through either increased crosslinks (advanced glycation end products, AGEs)or fragmentation. It is unclear which collagen structural change accounts for reduced strength. The dose-dependent effect of ionizing radiation on mechanical and biochemical properties of whole bones are not well understood, particularly for ex vivo doses ranging from 50 to 35,000 Gy.

Description: Unloading during spaceflight is known to adversely affect mammalian physiology. Mechanical stimulation is required for repair and regeneration by stem cell lineages to maintain tissue health and mass. CDKN1a/p21 functions as a potent cell cycle arrest molecule and we previously found that CDKN1a/p21 was overexpressed in mouse bone during 15-days of spaceflight on STS-131 and localized to osteoprecursor cells in the femur. Therefore, we hypothesized that altered expression of CDKN1a/p21 leads to an arrest of bone formation during spaceflight in response to altered load. To study CDKN1a/p21 and its role in stem cell-based tissue regeneration, we use a CDKN1a/p21 knockout (KO) mouse to investigate the impact on bone structure, osteoprogenitor proliferation, and mineralized nodule formation. We have shown that bone marrow stem cells isolated from juvenile (11-week-old) and skeletally mature (18-week-old) KO mice have an increased bone formation potential as evidenced by increased proliferation and mineralization rates. In addition, we have shown that juvenile KO mice display significantly increased bone volume fraction (BV/TV) relative to wildtype (WT) mice, but not in skeletally mature KO mice, indicating increased resorption and bone turnover in adult mice. To more closely examine age differences in the KO mouse, we will study a wider spectrum of mice ranging from 4 weeks to 12 months in age. To do this, we will analyze differences in bone morphometric parameters using MicroCT and osteoblastogenesis assays. The pelvis, femur, and tibia are key in distributing weight and we expect to see altered remodeling and stem cell potential with age. In combination with histomorphometry, these results will help elucidate the complex mechanisms underlying bone tissue maintenance and stem cell regeneration.

Description: No abstract available

Description: Goals of Food Production in Space: NASA Human Research Program (HRP) near-term food production systems - supplement key nutrients (Vitamins B1,C, K and potassium) that degrade to inadequate levels on exploration class missions; Study behavioral health aspect - caring for plants and improving the acceptability of the astronaut diet by supplementing it with fresh produce (currently anecdotal); Develop sustainable water delivery technologies - current TRL (Technological Readiness Level) 9 particulate based systems (i.e. used in research conducted in plant growth facilities APH (Advanced Plant Habitat), Veggie, BPS (Biomass Production System), LADA/SVET (Russian space plant chamber systems)) require resupply of bulky consumables and generate waste media.

Description: Long-term spaceflight leads to profound changes in multiple organs systems attributable to unloading and fluid shifts in microgravity. Future space explorations beyond low earth orbit will expose astronauts to space radiation, which may result in additional deficits that are not yet fully understood.The Space Life Sciences Research and Applications Division is hosting a lunch and learn briefing by Dr. Ruth Globus of the Ames Research Center. The topic is how living in space causes changes in the human body that resemble age-related diseases on earth (like osteoporosis), and how we experimentally explore coping responses.modulating the responses of bone to the challenges of spaceflight. This presentation will highlight how knowledge from studies on fundamental bone biology can inform the design of intervention strategies against spaceflight-induced bone loss.

Description: No abstract available