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  • wheat  (55)
  • RFLP
  • Springer  (84)
  • 1990-1994  (84)
  • 1991  (84)
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  • Springer  (84)
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  • 1990-1994  (84)
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  • 1
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA variation in geographic populations of Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera; Chrysomelidae) (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 483-485 
    Details
    ISSN: 1420-9071
    Keywords: Mitochondrial DNA ; RFLP ; Leptinotarsa decemlineata ; Colorado potato beetle ; population genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary This study demonstrates variability in restriction enzyme cleavage sites of mitochondrial DNA (mtDNA) among four popalations of Colorado potato beetle (CPB). A suite of three enzymes (EcoRI,HpaI,PstI) was sufficient to discriminate among the populations tested. Individuals heteroplasmic for restriction enzyme patterns were found in some populations. Variability in CPB mtDNA should prove useful in efforts to trace the origin and dispersal of the species in North America.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01959950
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of the incubation conditions on the production of patulin by Penicillium griseofulvum isolated from wheat (1991)
    Springer
    Mycopathologia 115 (1991), S. 163-168 
    Details
    ISSN: 1573-0832
    Keywords: Penicillium griseofulvum ; patulin ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sixty-four wheat samples from Spanish flour factories were screened for patulin and patulin-producing moulds. None of them was found to contain any patulin, whereas samples experimentally contaminated with this toxin proved it to be highly unstable. On the other hand, Penicillium griseofulvum was the only in vitro patulin-producing species found (19 samples). Mould growth in the samples was investigated by using yeast-sucrose medium (YES) and high-performance liquid chromatography (HPLC) to measure the amounts of toxin produced during 40 day's incubation at 20 and 28°C. The highest yield rate of patulin was obtained between the 20th and 30th day of incubation; such a rate, however, was very low throughout the vigorous growth phase, during the first 20 days of incubation. The more appropriate temperature for incubation and patulin production was 28 °C. We also investigated the influence of other incubation conditions in the yield and found stationary dark cultures to be more efficient that shaken or fermentation cultures in YES medium. The best patulin yield achieved was 11.9 mg in the culture broth and 6.3 mg in the mycelium from 100 ml of medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00462220
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of level of application on the residual value of superphosphate on a sandy soil in south-western Australia (1991)
    Springer
    Nutrient cycling in agroecosystems 29 (1991), S. 163-172 
    Details
    ISSN: 1573-0867
    Keywords: Superphosphate ; residual value ; sandy soil ; leaching of phosphorus ; lupins ; barley ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on deep, yellow, sandy soil near Badgingarra, Western Australia, the residual value of superphosphate applied one and two years previously was measured relative to freshly-applied superphosphate using yields of narrow-leafed lupin (Lupinus angustifolius), barley and wheat. In addition, soil samples were collected for measurement of bicarbonate-extractable soil P. This was also used to estimate the residual value of the superphosphate. For lupins and wheat, and for bicarbonate-extractable soil P, the residual value decreased with increasing level of application. For barley grain, the residual value was not significantly affected by the level of application. The decrease in residual value of superphosphate with increasing level of application is attributed to increased leaching of applied phosphorus (P) down the profile of the sandy soils as the level of application increases. This may reduce subsequent plant yields due to the delay in seedling roots reaching the P in the soil during the crucial early stages of plant growth. For lupins, the relationship between yield and the level of superphosphate applied was markedly sigmoidal. The relationship for wheat and barley was exponential. Consequently, at suboptimal levels of P application, lupins required about two to three times more P than wheat or barley to produce the same yield. However, lupins required less P to achieve near-maximum yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048956
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  • 4
    Electronic Resource
    Electronic Resource
    A segment of rye chromosome 1 enhances growth and embryogenesis of calli derived from immature embryos of wheat (1991)
    Springer
    Plant cell reports 10 (1991), S. 148-151 
    Details
    ISSN: 1432-203X
    Keywords: wheat ; rye ; embryogenesis ; growth ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the short arm of rye chromosome 1 (1RS) from Secale cereale var. Imperial on the growth and differentiation of callus cultures from wheat Triticum aestivum var. Chinese Spring immature embryos was analysed. This chromosome arm was found to stimulate both embryogenesis and the rate of growth of calli. Recombinant lines carrying segments of 1RS were used to delineate the regions of 1RS responsible for the tissue culture effects. The enhancement of embryogenesis and the stimulation of growth were shown to be associated with two distinct genetic regions of the chromosome arm; the former is located between the centromere and the Sec 1 locus, while the latter is situated in the immediate vicinity of the Sec 1 locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232047
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization of members of a pseudogene subfamily of the wheat α-gliadin storage protein genes (1991)
    Springer
    Plant molecular biology 16 (1991), S. 335-337 
    Details
    ISSN: 1573-5028
    Keywords: Triticum ; wheat ; endosperm ; gliadin ; pseudogene ; duplication ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020565
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  • 6
    Electronic Resource
    Electronic Resource
    Wheat ubiquitin gene exhibits a conserved protein coding region and a diverged 3′ non-coding region (1991)
    Springer
    Plant molecular biology 16 (1991), S. 907-908 
    Details
    ISSN: 1573-5028
    Keywords: ubiquitin ; wheat ; heat shock protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015082
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  • 7
    Electronic Resource
    Electronic Resource
    Site-directed mutagenesis and expression inEscherichia coli of WMAI-1, a wheat monomeric inhibitor of insect α-amylase (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1005-1011 
    Details
    ISSN: 1573-5028
    Keywords: α-amylase inhibitor ; expression inE. coli ; glycosylation versus activity ; insect α-amylase ; mutagenesis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wheat monomeric inhibitor WMAI-1 (syn. 0.28) produced inEscherichia coli using the pT7-7 expression ventor has the correct N-terminal sequence and the same electrophoretic mobility and specific activity towards the α-amylase from the insectTenebrio molitor as the native WMAI-1 isolated from wheat. This confirms that the native inhibitor is not glycosylated and contradicts claims that a putative glycosyl moiety was essential for inhibition. Thirteen mutants have been obtained at six different sites. Substitution of the highly conserved N-terminal S by the sequence ARIRAR increased the pre-incubation time required for maximum activity. A similar result was obtained by insertion of GPRLPW after position 4, while insertion of EPRAPW at the same position rendered the inhibitor inactive. The substitution D/EGPRL and insertions DGP or D, at position 58, produced complete inactivation. All other mutations had only minor effects on activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037140
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  • 8
    Electronic Resource
    Electronic Resource
    A Triticum aestivum cDNA clone encoding a low-molecular-weight heat shock protein (1991)
    Springer
    Plant molecular biology 17 (1991), S. 273-275 
    Details
    ISSN: 1573-5028
    Keywords: Tahsp17.3 ; low-molecular-weight HSP ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039504
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  • 9
    Electronic Resource
    Electronic Resource
    Cloning and sequencing of cDNAs encoding a pathogen-induced putative peroxidase of wheat (Triticum aestivum L.) (1991)
    Springer
    Plant molecular biology 16 (1991), S. 329-331 
    Details
    ISSN: 1573-5028
    Keywords: pathogen-induced ; peroxidase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here the complete amino acid sequence of a pathogen-induced putative peroxidase from wheat (Triticum aestivum L.) as deduced from cDNA clones representing mRNA from leaves infected with the powdery mildew fungus Erysiphe graminis. The protein consists of 312 amino acids, of which the first 22 form a putative signal sequence, and has a calculated pI of 5.7. Sequence comparison revealed that the putative wheat peroxidase is most similar to the turnip (Brassica rapa) peroxidase, with which it shares 57% identical and 13% conserved amino acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020563
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  • 10
    Electronic Resource
    Electronic Resource
    Restriction fragment length polymorphism analysis of CCDD genome species of the genus Oryza L. (1991)
    Springer
    Plant molecular biology 16 (1991), S. 831-839 
    Details
    ISSN: 1573-5028
    Keywords: RFLP ; Oryza ; rice ; genome evolution ; allotetraploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment length polymorphisms (RFLPs) were studied in fourteen accessions of CCDD genome allotetraploid wild rice species (Oryza latifolia, O. alta and O. grandiglumis). Fourteen nuclear RFLP markers previously mapped in AA genome-cultivated rice were used as probes. A phylogenetic tree, constructed by parsimony analysis based on RFLPs, grouped the accessions according to their geographic origin from Central or South America. Oryza alta, O. grandiglumis and one accession of O. latifolia grouped together as a subgroup, and our results suggested that the three taxa should be considered as populations of a single complex species. Duplicate loci, representing the two constituent genomes of the allotetraploid, were observed for most RFLP markers. By comparing RFLPs from the allotetraploids with those from a CC genome diploid wild species (O. officinalis), it was possible to detect RFLPs specific for both the CC and DD genomes of the allotetraploid. In inter-accession F2 populations, independent segregation of RFLP markers for CC and DD genomes was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015075
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  • 11
    Electronic Resource
    Electronic Resource
    Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.) (1991)
    Springer
    Plant molecular biology 16 (1991), S. 171-174 
    Details
    ISSN: 1573-5028
    Keywords: peroxidase gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic libary of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen-nor wound-induced in leaves but is constitutively expressed in roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017928
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  • 12
    Electronic Resource
    Electronic Resource
    Heterozygosity in tetraploid potatoes revealed by rDNA polymorphism analysis of their dihaploid progenies: a contribution to chromosome assignment (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1045-1054 
    Details
    ISSN: 1573-5028
    Keywords: chromosome assignment ; dihaploid progenies ; heterozygosity ; potato ; rDNA ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction map and organization of rDNA was inferred from analysis of dihaploid progenies of two tetraploid genotypes of cultivated potato. Each tetraploid genotype was characterized by a specific distribution of different types of rDNA repetition units on their four homologous chromosomesII. The genotypes were heterozygous and differed by the kind of units carried by each chromosomeII. Models for the generation of the observed organization are discussed and supported by first cloning studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037143
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  • 13
    Electronic Resource
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    Sequence analysis of a cDNA encoding a Group 3 LEA mRNA inducible by ABA or dehydration stress in wheat (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1073-1076 
    Details
    ISSN: 1573-5028
    Keywords: abscisic acid ; dehydration ; LEA ; water stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475–486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23 000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016078
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  • 14
    Electronic Resource
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    A novel proline-rich protein from wheat (1991)
    Springer
    Plant molecular biology 16 (1991), S. 663-670 
    Details
    ISSN: 1573-5028
    Keywords: gene expression ; proline-rich protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich proteins. The WPRP1 cDNA clone was used to determine the copy number and chromosomal location of the WPRP1 gene by restriction fragment length polymorphism analysis of wheat inbred lines. Although WPRP1 is encoded by a single-copy gene it is also a representative of a larger family of related sequences. RNA gel blot analysis showed that expression of WPRP1 is highest in rapidly growing tissue which together with its amino acid composition suggests a structural role for the encoded protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023430
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  • 15
    Electronic Resource
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    A wheat glutathione-S-transferase gene with transposon-like sequences in the promoter region (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1089-1091 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; glutathione-S-transferase ; transposon-like sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016083
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  • 16
    Electronic Resource
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    Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding the waxy protein (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1099-1101 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; waxy protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016086
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  • 17
    Electronic Resource
    Electronic Resource
    Structure and sequence of a wheat phosphoribulokinase gene (1991)
    Springer
    Plant molecular biology 17 (1991), S. 167-168 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; monocot gene ; phosphoribulokinase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036823
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  • 18
    Electronic Resource
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    Nucleotide sequence of a Triticum aestivum cDNA clone which is homologous to the 26 kDa chloroplast-localized heat shock protein gene of maize (1991)
    Springer
    Plant molecular biology 17 (1991), S. 255-258 
    Details
    ISSN: 1573-5028
    Keywords: Tahsp26.6 ; chloroplast-localized HSP ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039500
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  • 19
    Electronic Resource
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    Identification of an RFLP marker tightly linked to theHt1 gene in maize (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 393-398 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Helminthosporium turcicum race1 ; RFLP ; NILs ; Mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have identified tight linkage of an RFLP marker to theHt1 gene of maize that confers resistance to the fungal pathogenHelminthosporium turcicum race 1. This was accomplished by the use of four pairs of near isogenic lines (NILs; B73, A619, W153R, and CM105), each differing by the presence or the absence of the geneHt1. SinceHt1 maps to chromosome 2, 26 clones already mapped to this chromosome were labeled and probed against Southern blots of these NILs DNA digested with three restriction enzymes:EcoRI,BamHI, andHindIII. Six markers exhibited an RFLP for at least one pair of NILs. Presumptive linkage was further tested by analyzing the segregation of five of the six markers (one was monomorphic in the cross studied) and resistance toH. turcicum race 1 on 95 F2 individuals from the cross DF20 × LH146Ht. The results indicate a tight linkage between one of the DNA markers,UMC150B, and theHt1 gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00588588
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  • 20
    Electronic Resource
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    Bamboo germplasm screening with nuclear restriction fragment length polymorphisms (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 697-703 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; Bamboo ; Phyllostachys ; Chloroplast DNA ; Germplasm screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Bamboo species are difficult to identify because flowering material is seldom available and taxonomy is of necessity based on vegetative characters. To evaluate the utility of restriction fragment length polymorphism (RFLP) analysis in bamboo systematics and germplasm screening, a library of random genomic probes from a Phyllostachys nigra PstI library was constructed. Probes from the library were used to screen bamboo germplasm consisting mostly of temperate bamboos of the genus Phyllostachys. RFLP variation was abundant, and species-specific patterns were readily obtained. Chloroplast DNA showed little variation among the bamboo accessions analyzed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227313
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  • 21
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    Restriction fragment length polymorphism analysis of polymerase chain reaction products amplified from mapped loci of rice (Oryza sativa L.) genomic DNA (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 489-498 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; PCR ; Rice ; Inheritance ; Nonradioactive
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty mapped Indica rice genomic (RG) clones were partially sequenced from each end. From such sequence data, pairs of oligonucleotides were synthesized to act as primers for polymerase chain reaction (PCR) amplification of the corresponding loci in crude total DNA preparations. The PCR products from DNA of Indica varieties were of the sizes expected from the sizes of the corresponding RG clones. However, size polymorphisms were seen between PCR products from Indica and Japonica varieties, and among wildOryza species. Restriction fragment length polymorphism (RFLP) was observed between PCR products of Indica varieties simply by electrophoretic analysis of restricted products, without the need for Southern hybridization or radiolabelling. The RFLPs noted between varieties ARC6650 and Phalguna were inherited in recombinant inbred lines derived from a cross between them. The RFLPs were detectable in PCR products amplified from DNA extracted by a simple procedure from single seedlings or leaves, and revealed genetic heterogeneity in cultivated lines. An approach is described that is relevant to the acceleration of classical plant breeding through molecular techniques.
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    URL: http://dx.doi.org/10.1007/BF00588604
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  • 22
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    RFLP variation in diploid and tetraploid alfalfa (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 89-96 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; Alfalfa ; Genetic diversity ; Phylogenetic tree ; Gene duplication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alfalfa (Medicago sativa L.) is a major forage crop throughout the world. Although alfalfa has many desirable traits, continued breeding is required to incorporate pest resistances and other traits. We conducted this study to determine the amount of restriction fragment length polymorphism (RFLP) variability present within and between diploid and tetraploid alfalfa populations, and whether or not this variability is sufficient for construction of an RFLP map. Diploid plants from M. sativa ssp. falcata, ssp. coerulea, and ssp. sativa and tetraploid spp. sativa cultivars ‘Apollo,’ ‘Florida 77,’ and ‘Spredor 2’ were included. A total of 19 cDNA clones was probed onto genomic Southern blots containing DNA digested by EcoRI, HindIII, or BamHI. Phylogenetic trees were produced, based on parsimony analysis of shared restriction fragments. Evidence for extensive gene duplication was found; most probes detected complex patterns of restriction fragments. Large amounts of variation are present within all diploid subspecies. M. sativa ssp. falcata plants formed clusters distinct from ssp. sativa or ssp. coerulea plants, which were not distinctly clustered. Some M. sativa ssp. falcata plants were more similar to the other groups than to other plants within ssp. falcata. Variation among tetraploid cultivars showed that Florida 77 and Apollo had more similarities than either showed with Spredor 2. All three cultivars showed large within-population variation, with Apollo being the most diverse and Spredor 2 the least. Based on these results, development of an RFLP map at the diploid level appears possible. Also, differentiation of cultivars, particularly ones of divergent origin, seems possible based on RFLP patterns.
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    URL: http://dx.doi.org/10.1007/BF00229230
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  • 23
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    RFLP mapping of I1, a new locus in tomato conferring resistance against Fusarium oxysporum f. sp. lycopersici race 1 (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 22-26 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon esculentum ; L. pennellii ; Fusarium wilt ; RFLP ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance and linkage relationships of a gene for resistance to Fusarium oxysporum f. sp. lycopersici race 1 were analyzed. An interspecific hybrid between a resistant Lycopersicon pennellii and a susceptible L. esculentum was backcrossed to L. esculentum. The genotype of each backcross-1 (BC1) plant with respect to its Fusarium response was determined by means of backcross-2 progeny tests. Resistance was controlled by a single dominant gene, I1, which was not allelic to I, the traditional gene for resistance against the same fungal pathogen that was derived from L. pimpinellifolium. Linkage analysis of 154 molecular markers that segregated in the BC1 population placed I1 between the RFLP markers TG20 and TG128 on chromosome 7. The flanking markers were used to verify the assignment of the I1 genotype in the segregating population. The results are discussed with reference to the possibility of cloning Fusarium resistance genes in tomato.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231273
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  • 24
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    RFLP analysis of nuclear DNAs homologous with mitochondrial plasmid-like DNAs in cultivated rice (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 179-184 
    Details
    ISSN: 1432-2242
    Keywords: Plasmid-like DNA ; Rice ; Mitochondrial DNA ; RFLP ; Nucleo-mitochondrial DNA transmission
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary B1 and B2 are small, circular, mitochondrial plasmid-like DNAs found in male-sterile cytoplasm (cms-Bo) of rice. In this study, nuclear sequences homologous to these DNAs were investigated among a number of rice cultivars. Several copies of nuclear B1-and B2-homologous sequences were detected in all examined cultivars, regardless of the presence or absence of the B1 and B2 DNAs in mitochondria, indicating that the existence of the B1- and B2-homologous sequences in the rice nuclear genome was widespread. A restriction fragment length polymorphism (RFLP) was detected for both sequences, and we propose that these DNAs could be useful RFLP markers for the rice nuclear genome. To analyze these nuclear homologues genetically, segregation analysis of the RFLP was carried out in the F2 progenies of an Indica-Japonica rice hybrid. Of the B1 homologues, there were two nonallelic fragments, one specific to the Indica parent and the other to the Japonica. These results indicate that the B1 and B2 homologues were dispersed in the nuclear genome. The integration of B1-homologous DNA into the nuclear DNA may have occurred independently after sexual isolation of the Indica and Japonica rice varietal groups, or a intranuclear transposition of these sequences took place during the process of rice differentiation into the varietal groups.
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    URL: http://dx.doi.org/10.1007/BF00226210
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  • 25
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    Analyses of Phaseolus vulgaris L. and P. coccineus Lam. hybrids by RFLP: preferential transmission of P. vulgaris alleles (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 703-709 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus interspecific hybrid ; RFLP ; Beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphism (RFLP) was determined among P. vulgaris genotypes and Phaseolus species using 19 probes. The incidence of polymorphism was high (70–86%) between species, but relatively low (22–26%) between genotypes of P. vulgaris. Suitable probes were identified for the analysis of P. vulgaris and P. coccineus hybrids. The segregation pattern in F2 populations was Mendelian for two probes (LHB and VEE20) and non-Mendelian for GS-g, CHS, and CHI. Statistical analyses indicated gametic selection with preferential transmission of the P. vulgaris alleles, which may account for the selective recovery of P. vulgaris progeny types observed earlier. The available hybrids of P. vulgaris and P. coccineus and the high degree of interspecific RFLP will facilitate the construction of a linkage map for Phaseolus.
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    URL: http://dx.doi.org/10.1007/BF00226740
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    RFLP-based genetic map of rye (Secale cereale L.) chromosome 1R (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 174-178 
    Details
    ISSN: 1432-2242
    Keywords: Rye ; Genetic mapping ; RFLP ; Storage protein ; Isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A map of chromosome 1R of rye was constructed using 16 molecular and biochemical loci. From long arm to short arm, known-function loci were placed in the order: XAdh — XLee — Glu-R1[Sec-3] — XPpdk-1R — XEm-1R-1 — XEm-1R-2 — Centromere — XNor-R1 —Gpi-R1 — XGli-R1 [Sec-1a] along with six anonymous genomic and cDNA clones from wheat. The map, which spans 106 cM with 12 loci clustered in a 15-cM region around the centromere, shows reasonably good agreement with previously published maps for the centromeric region, whereas the XNor-R1 — Gpi-R1 region gives a much larger distance than previously reported.
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    URL: http://dx.doi.org/10.1007/BF00226209
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    RFLP maps of potato and their alignment with the homoeologous tomato genome (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 49-57 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; Potato ; Tomato ; Genetic maps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An RFLP linkage map of the potato is presented which comprises 304 loci derived from 230 DNA probes and one morphological marker (tuber skin color). The self-incompatibility locus of potato was mapped to chromosome I, which is homoeologous to tomato chromosome I. By mapping chromosome-specific tomato RFLP markers in potato and, vice versa, potato markers in tomato, the different potato and tomato RFLP maps were aligned to each other and the similarity of the potato and tomato genome was confirmed. The numbers given to the 12 potato chromosomes are now in accordance with the established tomato nomenclature. Comparisons between potato RFLP maps derived from different genetic backgrounds revealed conservation of marker order but differences in chromosome and total map length. In particular, significant reduction of map length was observed in interspecific compared to intraspecific crosses. The distribution of regions with distorted segregation ratios in the genome was analyzed for four potato parents. The most prominent distortion of recombination was found to be caused by the self-incompatibility locus.
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    URL: http://dx.doi.org/10.1007/BF00229225
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    Characterization of disomic addition lines Brassica napus-Brassica nigra by isozyme, fatty acid, and RFLP markers (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 43-49 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Genome ; Isozymes ; RFLP ; Fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six Brassica napus — B. nigra disomic addition lines were characterized by isozyme, fatty acid, and RFLP markers. The markers were arranged in six synteny groups, representing six of the eight chromosomes present in the B. nigra genome. Synteny group 1 displayed high levels of linoleic and linolenic acids in the seeds of the B. nigra parent. Synteny group 3 accumulated higher levels of eicosenoic and erucic acid than B. nigra. Three of the lines transmitted the alien chromosome to 100% of the progeny. The rest had variable transmission rates but all were above 50%. Most of the lines produced disomic addition plants in their progeny, suggesting pollen transmission of the alien chromosome. In addition to the marked lines, six others remained unmarked. These could be grouped into two classes according to their alien chromosome transmission. It is likely that they represent the two other B. nigra chromosomes that remained uncharacterized by the markers. No diploid individuals carrying B. nigra genome-specific markers were detected in the progenies studied.
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    URL: http://dx.doi.org/10.1007/BF00226110
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    RFLP mapping using near-isogenic lines in the soybean [Glycine max (L.) Merr.] (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 189-198 
    Details
    ISSN: 1432-2242
    Keywords: Glycine max ; Near-isogenic lines ; Molecular markers ; RFLP ; Linkage ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage between that molecular marker and the introgressed marker. In this study, we examined the utility of RFLPs as molecular markers for the NIL genemapping approach. The allelic status of fifteen RFLP loci was determined in 116 soybean RP/NIL/DP line sets; 66 of the ‘Clark’ RP type and 50 of the ‘Harosoy’ RP type. Of the 1740 possible allelic comparisons (116 NILs x 15 RFLP loci), 1638 were tested and 462 (33.9%) of those were informative (i.e., the RP and DP had different RFLP alleles). In 15 (3.2%) of these 462 cases the NIL possessed the DP-derived RFLP allele, leading to a presumption of linkage between the RFLP locus and the introgressed conventional marker locus. Two presumptive linkages, pK-3 — and pK-472 — Lf i, were subsequently confirmed by cosegregation linkage analysis. Although not yet confirmed, two other associations, pk-7 ab and pK-229 — y 9 seemed to be plausible linkages, primarily because the pk-7 — ab association was detected in two independently derived NILs and both markers of the pK-229 — y 9 association were known to be linked to Pb. The data obtained in this investigation indicated that RFLP loci were useful molecular markers for the NIL gene-mapping technique.
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    URL: http://dx.doi.org/10.1007/BF00215722
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    Molecular and cytological evidence of deletions in alien chromosomes for two monosomic addition lines of Brassica campestris-oleracea (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 221-226 
    Details
    ISSN: 1432-2242
    Keywords: RFLP ; Deletion mapping ; Genome evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A series of RFLP and isozyme markers were followed in the progenies of two alien addition lines of Brassica campestris-oleracea. One of the lines, carrying the C genome chromosome 4 as the alien chromosome, was surveyed for six markers. Fifty-four percent of the plants carrying alien chromosomes displayed all the expected makers, whereas the rest had one to five markers missing. The second line for C genome chromosome 5 displayed a similar behavior when surveyed for three markers. All three markers were transmitted together in 46% of the plants carrying alien chromosomes, whereas the rest carried only one or two of the markers. The loss of markers was associated with reduced chromosome size caused by deletions. The observed chromosome deficiencies permitted deletion analysis for a rough physical mapping and ordering of the markers on the two C genome chromosomes. The deletions observed may represent another mechanism for molding the chromosomes of the Brassica genomes during their evolution.
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    URL: http://dx.doi.org/10.1007/BF00215726
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    RFLP analysis of Zea mays callus cultures and their regenerated plants (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 227-232 
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    ISSN: 1432-2242
    Keywords: Callus cultures ; RFLP ; Zea mays ; Oryza sativa ; Regeneration ; Somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tissue culture of the Zea mays inbred line A188 resulted in the regeneration of plants having a high level of phenotypic variation compared to seed-grown control plants. To determine how such variation was induced and whether this could be related to specific in vitro culture methods, callus cultures were established and maintained on different, commonly used culture media. Plants were regenerated and the genomic DNA of callus cultures and regenerants analysed for RFLP differences. The results show that regardless of the gene probe used, callus formation resulted in significant deviations from the DNA pattern normally found in seed-grown control plants. Alterations in gene copy number also occurred. As differentiation and organogenesis began, the level of DNA variation fell, and most of the regenerated plants showed a genetic similarity to the controls; those with RFLP differences were the somaclonal variants.
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    URL: http://dx.doi.org/10.1007/BF00215727
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    A chromosome-specific DNA sequence which reveals a high level of RFLP in wheat (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 397-400 
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    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; Chromosome-specific ; DNA sequence ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An unusual genomic DNA clone, PSR454, was isolated from a partial genomic library of wheat. This sequence is moderately repeated and detects at least 30 related sequences, all located in a tight linkage block on the long arm of chromosome 3B. When used as a RFLP probe, PSR454 detects a high level of polymorphism between wheat varieties that carry the sequence. There is no detectable hybridisation to sequences in one-third of the varieties tested, providing an “on-off” polymorphism that can be detected on dot blots, rather than the more resource-consuming conventional Southern analysis.
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    URL: http://dx.doi.org/10.1007/BF00228682
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    RFLP variability in peanut (Arachis hypogaea L.) cultivars and wild species (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 565-570 
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    ISSN: 1432-2242
    Keywords: Peanut ; RFLP ; Plant breeding ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RFLP variability was studied in eight U.S. peanut cultivars, representing the four market types, and in 14 wild Arachis species accessions, using random genomic clones from a PstI library. Very low levels of RFLP variability were found among the allotetraploids, which included the U.S. cultivars and Arachis monticola, a wild species. The diploid wild species were very diverse, however. RFLP patterns of the allotetraploids were more complex than the diploids, and the two constituent genomes could usually be distinguished. On the basis of RFLP band sharing, A. ipaensis, A. duranensis, and A. spegazzinii appeared most closely related to the diploid progenitor species of the allotetraploids. A dendrogram of relationships among the diploid wild species was constructed based on band sharing.
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    Distribution and organization of a tandemly repeated 352-bp sequence in the oryzae family (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 57-64 
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    ISSN: 1432-2242
    Keywords: Cultivated rice ; Wild rice ; RFLP ; Satellite DNA ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 352-bp EcoRI fragment from rice DNA was cloned and shown to be a member of a tandem repeat. Sequence determination revealed homologies with human alpha satellite DNA and maize knob heterochromatin specific repeat. This 352-bp sequence is highly specific for the AA genome of rice. However, copy number and sequence organization are variable, depending on the accession analyzed. Several examples of amplification were observed in O. rufipogon and O. longistaminata. Use of resolutive polyacrylamide gel electrophoresis and 4-bp cutter enzymes allowed one to distinguish between the Indica and Japonica subtypes of O. sativa. The same method also discriminates between two groups of O. rufipogon, the presumed ancestor of O. sativa, suggesting that the present day Indica and Japonica subtypes originated independently from two O. rufipogon distinct populations.
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    URL: http://dx.doi.org/10.1007/BF00231278
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    α-Amylase structural genes in rye (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 771-776 
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    ISSN: 1432-2242
    Keywords: Secale cereale ; RFLP ; α-Amylase ; Genetics ; Isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rye α-Amy1, α-Amy2, and α-Amy3 genes were studied in the cross between inbred lines using wheat α-amylase cDNA probes. The α-Amy1 and α-Amy2 probes uncovered considerable restriction fragment length polymorphism, whereas the α-Amy3 region was much more conserved. The numbers of restriction fragments found and the F2 segregation data suggest that there are three α-Amy1 genes, two or three α-Amy2 genes, and three α-Amy3 genes in rye. These conclusions were supported by a simultaneous study of α-amylase isozyme polymorphism. The F2 data showed the three individual α-Amy1 genes to span a distance of 3cM at the locus on chromosome 6RL. The genes were mapped relative to other RFLP markers on 6RL. On chromosome 7RL two α-Amy2 genes were shown to be separated by 5 cM. Linkage data within α-Amy3 on 5RL were not obtained since RFLP could be detected at only one of the genes.
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    URL: http://dx.doi.org/10.1007/BF00227324
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    Genome-wide reduction in recombination of backcross progeny derived from male versus female gametes in an interspecific cross of tomato (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 173-178 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon ; Sex ; Crossing-over ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have determined that meiotic recombination differs between male and female gametes derived from the same plant. A single F1 plant was backcrossed to each of the parents, Lycopersicon esculentum and L pennellii, as the male (BCE) and female (BCP) parent, respectively. A total of 85 RFLP markers, covering more than 75% of the tomato genome, was used to construct a genetic map for both populations. Since both recurrent parents were homozygous, recombination measured in each population reflects crossing-over rates leading to male (BCE) and female (BCP) gametes. Comparisons were made by interval (genetic distance between two adjacent markers), by chromosome, and for the total length of the genome. Significantly less recombination was observed for male gametes at all levels. No significant relationship was found between areas of reduced recombination and approximate location to the centromere. That selection plays some role could not be eliminated, but no clear evidence was observed for single-locus selection as a major factor in the general reduction of crossing-overs in male gametes.
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    URL: http://dx.doi.org/10.1007/BF00226248
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    Characterization and organization of gene families at the Gli-1 loci of bread and durum wheats by restriction fragment analysis (1991)
    Springer
    Theoretical and applied genetics 83 (1991), S. 209-216 
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    ISSN: 1432-2242
    Keywords: Wheat ; Gli-1 loci ; Gliadins ; LMW glutenins ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Probes related to γ-gliadins and to the LMW subunits of glutenin were used to determine the complexity of the Gli-1 loci, by RFLP analysis of euploid and aneuploid lines of bread wheat cv Chinese Spring and durum wheat cv Langdon. The two probes hybridised to separate sets of fragments derived from chromosomes 1 A, 1 B and 1D. The fragments related to the LMW subunit probe had a total copy number in HindIII digests of about 35 in Chinese Spring and 17 in Langdon, with more fragments derived from chromosomes 1D. The fragments hybridising to the γ-gliadin probe could be divided into two classes, based on whether they hybridised to the whole probe at high stringency or to the 3′ nonrepetitive region at moderate stringency. The fragments that failed to hybridise under these conditions were considered to be related to ω-gliadins. The fragments related to γ — and co-gliadins had total copy numbers of about 39 and 16, respectively, in HindIII digests of Chinese Spring, and about 24 and 12, respectively, in Langdon.
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    RFLP mapping on potato chromosomes of two genes controlling extreme resistance to potato virus X (PVX) (1991)
    Springer
    Molecular genetics and genomics 227 (1991), S. 81-85 
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    ISSN: 1617-4623
    Keywords: Potato virus X ; Resistance genes ; RFLP ; Solanum tuberosum ; Genetic introgression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two different chromosomal locations of major genes controlling extreme resistance to potato virus X (PVX) were found by restriction fragment length polymorphism (RFLP) analysis of two populations segregating for the resistance. The resistance geneRx1 mapped to the distal end of chromosome XII, whereasRx2 was located at an intermediate position on linkage group V in a region where reduced recombination and segregation distortion have also been observed. These linkage anomalies were due to abnormal behaviour of the chromosome contributed by the resistant parent P34. The results presented were obtained using two different strategies for mapping genes of unknown location. One approach was the use of probes revealing polymorphic loci spread throughout the genome and resulted in the mapping ofRx1. The second approach was based on the assumption of possible linkage between the resistance gene and clone-specific DNA fragments introduced from a wild potato species.Rx2 was mapped by adopting this strategy.
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    The genetic locus controlling supernodulation in soybean (Glycine max L.) co-segregates tightly with a cloned molecular marker (1991)
    Springer
    Molecular genetics and genomics 228 (1991), S. 221-226 
    Details
    ISSN: 1617-4623
    Keywords: Soybean ; Symbiosis ; Nitrogen fixation ; RFLP ; Plant genome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetic locus (nts) controlling nitrate-tolerant nodulation, supernodulation, and diminished autoregulation of nodulation of soybean (Glycine max (L.) Merill) was mapped tightly to the pA-132 molecular marker using a restriction fragment length polymorphism (RFLP) detected by subclone pUTG-132a. The nts (nitrate-tolerant symbiotic) locus of soybean was previously detected after its inactivation by chemical mutagenesis. Mutant plant lines were characterized by abundant nodulation (supernodulation) and tolerance to the inhibitory effects of nitrate on nodule cell proliferation and nitrogen fixation. The large number of RFLPs between G. max line nts382 (homozygous for the recessive nts allele) and the more primitive soybean G. soja (P1468.397) allowed the detection of co-segregation of several diagnostic markers with the supernodulation locus in F2 families. We located the nts locus on the tentative RFLP linkage group E about 10 cM distal to pA-36 and directly next to marker pA-132. This very close linkage of the molecular marker and the nts locus may allow the application of this clone as a diagnostic probe in breeding programs as well as an entry point for the isolation of the nts gene.
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    Role of bacterial cyanide production in differential reaction of plant cultivars to deleterious rhizosphere pseudomonads (1991)
    Springer
    Plant and soil 133 (1991), S. 93-100 
    Details
    ISSN: 1573-5036
    Keywords: cultivars ; growth inhibition ; hydrogen cyanide ; lettuce ; Pseudomonas fluorescens ; volatile metabolites ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lettuce and wheat cultivars, differing in reaction to root inoculation with plant growth-inhibitory bacteria, were tested for sensitivity to (i) gaseous metabolites produced by deleterious, cyanogenic isolates of Pseudomonas fluorescens, and to (ii) pure cyanide. Reactions were read as shoot and/or root elongation after exposure of seedlings to the volatiles in vitro. Lettuce cultivar Salad Bowl was significantly less sensitive than cv. Montana, both to bacterial volatiles and to pure cyanide, and a similar difference between these cultivars was also obtained in greenhouse experiments where bacteria were inoculated directly on the roots. Cultivar differences were, however, not recorded, either in vitro or in the greenhouse, when the bacteria were grown on a medium which did not support cyanide production. In wheat, a difference in sensitivity to bacteria-produced volatiles was recorded between two cultivars (Drabant and Besso) differing in reaction to bacterial inoculation, but in contrast to lettuce cultivars, the wheat cultivars tested did not react differentially to pure cyanide. The results suggest that in lettuce differential sensitivity to cyanide is one factor behind cultivar differences in reaction to the bacteria tested, even though bacterial metabolites other than cyanide may contribute to the plant growth inhibition recorded. In wheat, however, differential cultivar responses to these bacteria could not be shown to be related to cyanide.
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    Variation for root traits and their genetic control in spring wheat (1991)
    Springer
    Euphytica 59 (1991), S. 1-8 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; root ; combining ability ; correlation ; dry matter ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat root characters which influence vital plant processes have scarcely been explored for their genetic control. This study was conducted to i) examine the diversity of root traits and associated shoot traits in spring wheat (Triticum aestivum L.) cultivars; ii) study the nature of genetic control of selected traits; and iii) examine associations among root and shoot traits. Three experiments were conducted in the greenhouse with plants grown in the vermiculite medium in clear plastic tubes. In the first experiment, 42 spring wheat cultivars were grown for three weeks and measurements were taken on root length, leaf length, root number, leaf number, root dry weight, and top dry weight. In the second study, 15F1's originating from a partial diallel mating of six cultivars along with the parents were evaluated for 4 weeks. The data on root length, leaf length, and root number were subjected to diallel analysis according to Griffing's method 4, fixed model. A third experiment consisted of studying 2 F2 populations with 141 plants per population. In the first study the 42 cultivars exhibited a wide range of variation for all six traits. Diallel analysis revealed significant effects of both general combining ability (GCA) and specific combining ability (SCA) for root length, leaf length, and root number. Parents with high GCA estimates were identified. Significant positive correlation coefficients were found among root and shoot traits. Analysis of F2's for root length indicated quantitative nature of inheritance of root length.
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    Hybridization barriers between wheat and rye: In vitro pollen assays and electrophoretic survey (1991)
    Springer
    Euphytica 52 (1991), S. 147-153 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; hybridization ; pollen-tube-growth ; wide-crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five- to seven-day-old ovaries from Triticum aestivum vars Hope (non-crossable), Chinese Spring (CS, crossable) and the chromosomal substitution line Chinese Spring/Hope 5B (CS/H5B) were used to test the hypothesis that an ovarian substance inhibits rye (Secale cereale var Wrens) pollen tube growth. In vitro assays on a semi-solid medium demonstrated that a soluble, dialyzed lysate from Hope or CS/H5B ovaries inhibited rye pollen tube elongation significantly more than a similar lysate from CS ovaries (26%, 13% and 5% respectively compared to controls). When only the 20 longest tubes from each sample were included in the analysis the relationship was maintained (33%, 22% and 15% for Hope, CS/H5B and CS respectively). Heating the Hope lysate at 100° C for 10 min reduced the inhibition from 26% to 14% for all pollen tubes measured (n = 51 per sample) and from 33% to 20% when only the 20 longest pollen tubes per sample were analyzed. Isoelectric focusing followed by sodium dodecyl sulfate polyacrylamide electrophoresis (IEF/SDS-PAGE) and non-equilibrium pH gradient electrophoresis (NEPHGE) revealed several differences in the polypeptide profiles of soluble lysates from vars Hope, CS and CS/H5B. While two striking differences were observed, a 50 kilodalton (kD) polypeptide with an isoelectric point (pI) of approximately 8.5 and 100 kD polypeptide (pI∼4) from CS lysates which were considerably reduced in lysates of Hope and CS/H5B, none were obviously associated with the Hope 5B chromosome and therefore cross-incompatibility.
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    Crossability of some bread wheat landraces and improved cultivars from western Himalayas with rye (1991)
    Springer
    Euphytica 53 (1991), S. 137-141 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; crossability ; Himalayas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crossability of 62 bread wheat accessions (14 landraces from Himachal Pradesh and 48 others) was examined with rye. The 3 rye cultivars did not differ in their relative crossability with 4 of the wheat accessions studied. On the other hand, the wheat cultivars differed greatly among themselves in their crossability with rye. Most of the wheat cultivars showed poor (〈10%) crossability. Two of the 14 landraces from Himachal Pradesh were found to be free from the crossability inhibitors as they showed very high (〉50%) crossability, whereas none of the other 48 cultivars studied was so.
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    Major gene control of tolerance of bread wheat (Triticum aestivum L.) to high concentrations of soil boron (1991)
    Springer
    Euphytica 55 (1991), S. 217-228 
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    ISSN: 1573-5060
    Keywords: boron ; inheritance ; tolerance ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic control of tolerance of wheat to high concentrations of soil boron was studied for five genotypes. Each genotype represented one of five categories of response to high levels of boron, ranging from very sensitive to tolerant. Tolerance to boron was expressed as a partially dominant character, although the response of an F1 hybrid, relative to the parents, varied with the level of boron applied. The F1 hybrids responded similarly to the more tolerant parent at low B treatments and intermediate to the parents at higher treatments. Ratios consistent with monogenic segregation were observed for the F2 and F3 generations for the combinations (WI*MMC) × Kenya Farmer, Warigal × (WI*MMC) and Halberd × Warigal. The three genes, Bo1, Bo2 and Bo3, while transgressive segregation between two tolerant genotypes, G61450 and Halberd, suggested a fourth locus controlling tolerance to boron.
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    URL: http://dx.doi.org/10.1007/BF00021242
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    Pedigree analysis of the origin of manganese tolerance in Canadian spring wheat (Triticum aestivum L.) cultivars (1991)
    Springer
    Euphytica 56 (1991), S. 107-120 
    Details
    ISSN: 1573-5060
    Keywords: Canada ; genotypic differences ; manganese toxicity ; manganese tolerance ; origin ; pedigree ; screening ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Breeding wheat (Triticum aestivum L.) for tolerance to manganese (Mn) might be in some cases more feasible and economical than use of soil amendments. As part of research on the heritability of Mn tolerance, a study on the level of Mn tolerance in Canadian wheat cultivars and its probable origin was accomplished by analysis of cultivar pedigrees and drawing phylogenetic maps to discern filial relationships. Cultivar tolerance to Mn was determined by relative root weight (RRW) in solution culture in the presence of 500 μM Mn. A total of 91 cultivars were screened, 76 of which were Canadian. These data, together with data from another 28 cultivars reported in the literature, were used to draw two pedigree maps, a map for Canadian cultivars only, and a map for the Mn-tolerant Canadian cultivars Norquay and Laura. Results indicated a range of tolerance to Mn among Canadian cultivars. Manganese tolerance, found in either Canadian or foreign germplasm, and of either recent or older selection or origin, seems to have originated from land races from Rio Grande do Sul, the southernmost state of Brazil. Tolerance may have been introduced into Canadian germplasm directly by the use of Brazilian cultivars as parents, or indirectly by the introduction of Mexican germplasm with Brazilian parentages. This information will help the plant breeder to develop plant breeding systems, and may also help in the study of the mechanisms for Mn tolerance in wheat.
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    URL: http://dx.doi.org/10.1007/BF00042053
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    Greenhouse evaluation of red winter wheats for resistance to the Russian wheat aphid (Diuraphis noxia, Mordvilko) (1991)
    Springer
    Euphytica 57 (1991), S. 221-225 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Diuraphis noxia ; Russian wheat aphid ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Russian wheat aphid (Diuraphis noxia, Mordvilko) (RWA) is responsible for significant economic damage to cereal crops in arid and semi-arid environments. In this research 20 red winter wheats originating from Iran were evaluated for resistance to RWA. Leaf rolling, leaf folding, and leaf chlorosis were measured using 0 to 3 scales. An overall mean damage score was calculated as the average of the three measured damage symptoms. Plants from seven central Asian accessions (PI222666, PI222668, PI225226, PI225267, PI225271, PI243630, and PI243642) had mean damage scores significantly lower (p 〈 0.001) than ‘Stephens’ wheat (RWA susceptible) and not significantly different from ‘Border’ oat (RWA resistant). These results are consistent with previous studies which found a high frequency of resistant wheats collected from the central Asian region.
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    URL: http://dx.doi.org/10.1007/BF00039668
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    Breeding for hard red winter wheat cultivars adapted to conventional-till and no-till systems in northern latitudes (1991)
    Springer
    Euphytica 58 (1991), S. 57-63 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; cold hardiness ; cultivar x tillage interaction ; tillage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Conservation tillage in the Great Plains of North America has increased in recent years, in part, to control soil erosion, increase soil water storage and reduce production costs. No-till production is recommended for winter wheat (Triticum aestivum L.) in northern latitudes for the purpose of trapping snow and protecting the crop from freezing temperatures. A 5 year field study was conducted at four North Dakota locations from 1984–85 through 1988–89 to determine if significant cultivar x tillage interactions existed for grain yield of winter wheat. Fourteen hard red winter wheat cultivars were planted in both conventional-till and no-till systems. Significant cultivar x tillage interactions were obtained for grain yield from both a weighted analysis across all environments and from individual analyses of 12 of the 16 environments in which wheat survived under both tillage systems (P〈0.05). Regression of cultivar yield on mean environment yield produced b values that varied little between conventional till and no-till for 10 of the 14 cultivars. The top five yielding cultivars were the same for both tillage systems and had b values 〉 1.0. Although the cultivar x tillage interaction may be a result of differential winter survival rather than tillage per se, this study suggests that selection for winter wheats with superior yield performance can be conducted under either no-till or conventional-till systems.
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    URL: http://dx.doi.org/10.1007/BF00035340
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    Genes for resistance to stripe rust in four spring wheat varieties (1991)
    Springer
    Euphytica 58 (1991), S. 171-181 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four spring wheat (Triticum aestivum L.) varieties differing in origin and reaction in the seedling stage to pathotype CDL-6 (extant in California) were intercrossed and examined in greenhouse conditions in F1, F2, and F3 generations. Digenic and transgressive segregation was found in all crosses. The four varieties each had infection types (1 immune, 9 susceptible) and putative resistance genes as follows: ‘Anza’, IT 7, YrA; ‘Glennson 81’, IT 2, Yr9; ‘Yecora Rojo’, IT 6, YrC; and ‘Ollanta’, IT 4–6, YrL. ‘Anza’ was classified as susceptible, ‘Yecora Rojo’ and ‘Ollanta’ as intermediate in seedling resistance, and ‘Glennson 81’ as resistant in the seedling stage.
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    URL: http://dx.doi.org/10.1007/BF00022818
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    In vitro culture variation of wheat and rye caused by genes affecting plant growth habit in vivo (1991)
    Springer
    Euphytica 61 (1991), S. 233-240 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; day-length insensitivity ; semi-dwarfness ; tissue culture response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of genes affecting the ‘plant growth habit’ in wheat (Rht8 and Ppd1) and rye (ct1 and ct2) on tissue culture response was studied using immature embryos. Whereas the semi-dwarfing gene Rht8 seems to promote only a minor effect, the day-length sensitive allele ppd1 determined a major increase in callus growth and regeneration ability. With regards to their tissue culture efficiency, the four alleles studied could be ranked as follows: ppd1〉Rht8〉rht8〉Ppd1. In contrast to wheat, the GA insensitive semi-dwarfing genes of rye (ct1 and ct2) appear not to influence in vitro response.
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    Organic nitrogen content and nitrate and nitrite reductase activities in tritordeum and wheat grown under nitrate or ammonium (1991)
    Springer
    Plant and soil 135 (1991), S. 251-256 
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    ISSN: 1573-5036
    Keywords: nitrate reductase ; nitrite reductase ; organic nitrogen ; tritordeum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tritordeum is a fertile amphiploid derived from durum wheat (Triticum turgidum L. conv. durum) × a wild barley (Hordeum chilense Roem. et Schultz.). The organic nitrogen content of tritordeum grain (34 mg g-1 DW) was significantly higher than that of its wheat parent (25 mg g-1 DW). Leaf and root nitrogen content became higher in tritordeum than in wheat after four weeks of growth, independently of the nitrogen source (either NO3 - or NH4 +). Under NO3 - nutrition, tritordeum generally exhibited higher levels of nitrate reductase (NR) activity than wheat. Nitrite reductase (NiR) levels were however lower in tritordeum than in its wheat parent. In NH4 +-grown plants, both NR and NiR activities progressively decreased in the two species, becoming imperceptible after 3 to 5 weeks of growth. Results indicate that, in addition to a higher rate of NO3 - reduction, other physiological factors must be responsible for the greater accumulation of organic nitrogen in tritordeum grain.
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    URL: http://dx.doi.org/10.1007/BF00010913
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    Chlorophyll content and leaf elongation rate in wheat seedlings as a measure of manganese tolerance (1991)
    Springer
    Plant and soil 136 (1991), S. 1-9 
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    ISSN: 1573-5036
    Keywords: chlorophyll ; genotypic differences ; leaf elongation rate ; Mn toxicity ; Mn tolerance ; screening ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract After aluminum toxicity, manganese (Mn) toxicity is probably the second most important growth limiting factor in acid soils. The purpose of this study was to determine the feasibility of using chlorophyll content and leaf elongation rate (LER) for regrowth of Mn stressed seedlings as a rapid seedling based screening bioassay for Mn tolerance in segregating populations of wheat (Triticum aestivum L.). In one experiment, chlorophyll was determined for the cultivars Norquay (Mn-tolerant) and Columbus (Mn-sensitive) subjected to twelve Mn levels (2 to 2000 μM) in nutrient solutions. As Mn concentration increased, chlorophyll ‘a’ and ‘b’ contents of the Mn-tolerant cultivar decreased up to 9%, while in the Mn-sensitive cultivar it was reduced by as much as 43%. The chlorophyll ‘a/b’ ratio did not differ among Mn concentrations for either cultivar. In a second experiment, chlorophyll content and LER for regrowth of Mn stressed seedlings (1000 μM) was determined for Columbus and Katepwa (Mn-sensitive), Oslo (Mn-intermediate), and Norquay and Laura (Mn-tolerant). Manganese tolerance as assayed by chlorophyll ‘a’ and ‘b’ and LER was significantly correlated with Mn tolerance as assayed by the relative root weight methodology (RRW). Thus, chlorophyll content of Mn-stressed seedlings and LER of seedling regrowth appear to be suitable techniques for screening unreplicated selections of segregating populations for tolerance to Mn.
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    URL: http://dx.doi.org/10.1007/BF02465214
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    Distribution of magnesium in wheat (Triticum aestivum L.) in relation to supply (1991)
    Springer
    Plant and soil 136 (1991), S. 183-193 
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    ISSN: 1573-5036
    Keywords: diagnosis ; distribution ; magnesium deficiency ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aims of this study were to describe the distribution of magnesium (Mg) and its retranslocation within wheat, in order to develop diagnostic procedures for Mg deficiency. Plants were grown in solution culture with both constant supply (0, 5, 10, 20, 40, 80, 160 μMMg) and discontinued supply (40 μM and 160 μM decreased to nil). Magnesium was depleted from old leaves when Mg supply to the roots was halted. However, initial deficiency symptoms occurred on young leaves under constant but inadequate supply, contrasting with previous reports. Magnesium concentrations were also lower in young leaves compared to old leaves. Symptoms of yellowing and necrosis occurred if the leaf tissue contained 〈1194 μgg−1, irrespective of leaf age. The minimum Mg concentration in whole shoots associated with maximum shoot weight was 932 μgg−1; for the youngest emerged blade (YEB) it was 861 μgg−1. Symptoms were apparent on the young leaf before a reduction in shoot weight was measurable. The concentration of Mg in the YEB and whole shoot were better related to solution Mg concentration than was the Mg concentration in the old leaf.
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    The fate of carbon in pulse-labelled crops of barley and wheat (1991)
    Springer
    Plant and soil 136 (1991), S. 205-213 
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    ISSN: 1573-5036
    Keywords: barley ; carbon ; 14C ; exudates ; partitioning ; respiration ; rhizodeposition ; root ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (cv. Gutha) and barley (cv. O'Connor) were grown as field crops on a shallow duplex soil (sand over clay) in Western Australia with their root systems contained within pvc columns. At four stages during growth, the shoots were pulse-labelled for 1.5h with14CO2; immediately prior to labelling, the soil was isolated from the shoot atmosphere by pvc sheets. After labelling, the soil atmosphere was pumped through NaOH to trap respired CO2 and after 2.5, 5, 7.5 and 24 h from the start of labelling, columns were destructively sampled to recover14C from the roots, soil and shoot. Both species showed similar patterns of14C distribution and changes in distribution through the growing season. During early tillering, 15–25% of the14C recovered after 24 h had been respired by the roots and rhizosphere, 17–27% was retained in the roots, 0.4–1.8% was recovered as water-soluble14C in the soil and the remainder (45–67%) was present in the shoot. These percentages changed during growth so that during grain filling only 2–3% of the14C recovered after 24 h was as respired CO2, 2–6% was in the roots, 0.2% was in the soil and over 90% was in the shoot. The distribution of14C in components of the soil-plant system changed during the 24 h after labelling with the most rapid changes occurring generally during the first 7.5 h after labelling. Using growth measurements from adjacent plots, the amounts of C added to the soil were estimated for the whole season. Carbon input to the soil was about 48 gC m−2 for wheat and 58 gC m−2 for barley; the crops produced total shoot dry matter of 494 (wheat) and 735 g m−2 (barley). Of the C input to the soil, 27.8% (wheat) and 40.3% (barley) was as respired C and only 3.3 (wheat) and 4.1% (barley) was collected as exudate (water-soluble material).
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    URL: http://dx.doi.org/10.1007/BF02150051
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    Cytoplasmic genetic diversity in bananas and plantains (1991)
    Springer
    Euphytica 52 (1991), S. 19-23 
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    ISSN: 1573-5060
    Keywords: Musa acuminata ; Musa balbisiana ; banana ; plantain ; cytoplasm ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Concerns over yield declines in bananas and plantains due to the spread of Black Sigatoka disease in Musa have drawn attention to the collection of Musa germplasm and its use in conventional and biotechnological improvement programs. This report demonstrates the use of chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) for differentiating cytoplasms of various Musa clones. DNA was extracted from lyophilized leaf blade tissue and digested with either Eco R1, Hind III, Bam H1 or Pst I. Southern blots onto nylon membranes were probed with radioactively labeled heterologous orchid and lettuce cpDNA fragments. Among the 14 Musa clones examined, a single balbisiana and four acuminata-type cytoplasms were differentiated. The ability to distinguish between cytoplasms and to place plants within a cytoplasmic grouping demonstrates the usefulness of RFLP technology in evaluating diversity and determining the ancestry of Musa clones.
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    Components of variation, heritabilities and correlations for indices of sprouting tolerance and seed dormancy in Triticum spp. (1991)
    Springer
    Euphytica 52 (1991), S. 221-229 
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    ISSN: 1573-5060
    Keywords: Triticum ; wheat ; sprouting tolerance ; dormancy ; variation ; heritability ; correlation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Germination of wheat (Triticum spp) kernels prior to harvest reduces the economic value of grain. Losses attributable to pre-harvest sprouting could be reduced by developing sprouting tolerant (ST) cultivars if heritable variation exists for this trait. Objectives of this study were to compare various assays that measure ST, seed dormancy (SD), and alpha-amylase activity, and to divide the total variation for these traits into parts relating to genetic differences, genotype-year interaction, and residual variation. Twenty-six hexaploid wheat genotypes (Triticum aestivum L.) (eight red-kernelled and eighteen white-kernelled), and three white-kernelled tetraploid wheat genotypes (T. turgidum L. var. durum) were subjected to various assays that measure ST, SD, and alpha-amylase production using artificial wetting treatments. Genotype effects accounted for 44% to 90% of the phenotypic variation. The genetic component of variation, when expressed as a proportion of the total variation, was 2 to 6 times greater than the proportion attributable to error. Heritability expressed on a genotype mean basis was highly significant for all variables and ranged from 0.59 to 0.93. The phenotypic correlation for any ST or SD trait or alpha-amylase activity measured on samples collected at two dates (T1 and T2 = T1+14 days) was highly significant and positive. The phenotypic correlation matrix among assays performed on the unthreshed spikes, germination tests and alpha-amylase activity at both T1 and T2 were positive and highly significant. Increased ST is an attainable objective, and direct selection for ST using an artificial wetting treatment is an appropriate breeding strategy.
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    URL: http://dx.doi.org/10.1007/BF00029399
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    Application of a set of 14 c-DNA probes from wheat to detect restriction fragment length polymorphism (RFLP) in barley (1991)
    Springer
    Euphytica 53 (1991), S. 115-119 
    Details
    ISSN: 1573-5060
    Keywords: barley ; Hordeum vulgare ; RFLP ; chromosome arm ; molecular marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A set of 14 probes from wheat cDNA clones was used to search for restriction fragment length polymorphism (RFLP) in six barley lines. The degree of polymorphism among the lines varied greatly between probes and between the various restriction enzymes. Two probes revealed a high degree of polymorphism in all probe/enzyme combinations. Seven of 14 probes did not reveal RFLP. The average level of polymorphism based on all 840 pairwise comparisons was 14.0%, which is higher than has been reported in wheat, but lower than in maize, rice, potato and lettuce. Most of the probes that detected RFLP correspond to sites on the long arms of wheat chromosomes.
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    URL: http://dx.doi.org/10.1007/BF00023791
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    A comparison of methods for identifying and surveying the presence of 1BL.1RS translocations in bread wheat (1991)
    Springer
    Euphytica 54 (1991), S. 45-53 
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    ISSN: 1573-5060
    Keywords: Triucum aestivum ; wheat ; storage protein ; bread-making quality ; N-banding PA ; subtilisin inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four methods for detecting 1BL. 1RS translocations in bread wheat have been compared winter wheat cultivars: N-banding of mitotic metaphase chromosomes, sodium lactate electrophoresis at pH 3.1, sodium dodecyl sulphate-polyacrylamide gel electrophorests under conditions, and a recently characterised protein, subtilisin inhibitor, separated by isoelectric N-banding was much the most labour intensive method, and, of the three electrophoretic recommend the use of subtilisin inhibitor, which is at least as easy to interpret as the other is often faster for screening purposes. The sources of the 1BL. 1RS translocation in this matenal Avrora. Kavkaz and Skorospelka 35, which have been extensively used as parents in breeding programmes. Out of 59 cultivars that include a line carrying the 1BL. 1RS pedigree. 23 of them did not carry the translocation; thus the effect on plant phenotype of insufficient to guarantee its selection during breeding.
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    Mass selection of wheat for grain filling without transient photosynthesis (1991)
    Springer
    Euphytica 54 (1991), S. 111-116 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; drought resistance ; yield ; selection ; grain filling ; kernel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Post-anthesis chemical desiccation of wheat (Triticum aestivum L.) plants in the field eliminates transtent photosynthesis by killing all green tissues, thus revealing the plant's capacity for grain filling from stored stem reserves, as the case is for post-anthesis stress such as drought or leaf diseases. This study was conducted to investigate whether mass selection for large kernels under chemical desiccation would lead to the improve ment of grain filling in the absence of transient photosynthesis. Six crosses of common spring wheat were subjected to three cycles of mass selection from F2 through F1 when selection was performed for large kernels by sieving grains from plants that were erther chemically desiccated after anthesis, or not (controls). The resulting 36 bulks (six crosses by three selection cycles by two selection environments) were compared with their respective F2 base populations, when tested with and without chemical desiccation. Selection for large kernels under potential conditions (without chemical desiccation) did not improve kernel weight under potnetial conditions, evidently because these materials were lacking in genetic variation for kernel weight under potential conditions. In four of the crosses, 3rd cycle selection for large kernels under potential conditions decreased kernel weight under chemical desiccation. On the other hand, selection for large kernels under chemical desiccation was effective in improving kernel weight and test weight under chemical desiccation, depending on the cross and the selection cycle, with no genetic shift in mean days to heading or mean plant height. Selection for large kernels under chemical desiccation was also effective in some cases in increasing kernel weight under potential conditions. The results are interpreted to show that selection under potential conditions and under chemical desiccation operate on two different sources for grain filling, namely transient photosynthesis and stem reserve utilization, respectively. In order to expose genetic variability for stem reserve utilization to selection pressure, transient photosynthesis must be eliminated, as done by chemical desiccation in this study.
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    Gliadin allele composition of Yugoslav winter wheat cultivars (1991)
    Springer
    Euphytica 54 (1991), S. 285-295 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; gliadins ; APAGE ; multiple allelism ; variation ; Yugoslavia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The complete gliadin allele composition of 57 Yugoslav common winter wheat cultivars was studied. Large differences were found in gliadin genotypes among cultivars bred at different Yugoslav breeding centres as well as between early and recent wheats bred in the Novi Sad breeding centre. Yugoslav wheats have limited variation in gliadin alleles, of which Gli-A1a, Gli-B1b, Gli-B1l, Gli-D1b, Gli-A2e, Gli-B2b, Gli-D2a are shown to be the most frequent. Examples of favoured alleles to new cultivars are described. 40% of the studied wheat cultivars have natural biotypes differing in gliadin allele composition. The frequency of cultivars with the biotypes differs greatly among breeding centres.
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    Assessment of the degree of genetic variation in beet based on RFLP analysis and the taxonomy of Beta (1991)
    Springer
    Euphytica 55 (1991), S. 1-6 
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    ISSN: 1573-5060
    Keywords: Beta vulgaris ; beet ; RFLP ; Beta taxonomy ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Clones derived from Beta vulgaris and Beta maritima were assayed for their ability to detect restriction fragment length polymorphisms (RFLPs) in different beet accessions. The clones able to detect polymorphism were used as genetic markers to assess the degree of genetic variation existing between and within different species of the genus Beta. The data support the current taxonomy of the Beta vulgaris section, while the great genetic similarity found between Beta webbiana and Beta procumbens indicates that they could belong to the same species. Enough variation was found between Beta vulgaris cultivars, allowing the isolation of a sufficient number of genetic markers for the construction of detailed genetic maps.
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    Comparison of resistance of triticale, wheat and spelt to septoria nodorum blotch at the seedling and adult plant stages (1991)
    Springer
    Euphytica 55 (1991), S. 43-48 
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    ISSN: 1573-5060
    Keywords: adult plant resistance ; seedling resistance ; Septoria nodorum ; septoria nodorum blotch ; X Triticosecale ; triticale ; Triticum aestivum ; wheat ; T. spelta ; spelt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary On average, the cereal species studied were susceptible to septoria nodorum blotch (SNB), except for spring triticale on leaf and head and winter titicale on leaf, that appeared to be significantly more resistant, than the other ones. In all three species the SNB response of the adult plants was to a limited extent only predicted by the reaction on first leaf seedlings. In most cases it was impossible to predict the response to SNB of adult plants on the basis of seedling reaction. Correlations between the adult plant stage and the seedling stage, or detached seedling leaves, appears not to be sufficient for use in practical breeding work. A reversal of reaction to SNB was even found between the above growth stages in studied spring and winter wheat varieties.
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    Combining ability analysis for harvest index in winter wheat (1991)
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    Euphytica 55 (1991), S. 229-234 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; harvest index ; diallel analysis ; genotypic and phenotypic correlations ; assimilate partitioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cereal breeders have used harvest index (HI) as a selection criterion in segregating generations to identify physiologically superior lines with improved partitioning of total assimilate into grain. Information on combining ability for HI of the hard red winter wheat (Triticum aestivum L.) cultivars of the Southern Great Plains is not available. A study was undertaken to examine HI of seven genetically diverse winter wheat parents, evaluate their general combining ability (GCA) and specific combining ability (SCA) effects, and study correlations of HI with several agronomic traits. The seven parents were crossed in a half-diallel mating design to produce 21 crosses. The F1's, their F2 progenies, and the parents were evaluated in replicated field tests at Stillwater and at Lahoma, OK. The combining ability analysis was performed using Griffing's Method 4, Model 1. The results showed significant variation among parents for HI. The GCA and the SCA effects were mostly inconsistent between generations and between environments. However, parents with consistently high HI and positive GCA estimates were identified. The progeny with high HI mostly resulted from parents with high GCA estimates. The correlations between HI and agronomic traits indicated that improvement in HI should also result in high grain yield, early maturity, and short plant height.
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    URL: http://dx.doi.org/10.1007/BF00021243
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    Additive and epistatic effects of allelic variation at the high molecular weight glutenin subunit loci in determining the bread-making quality of breeding lines of wheat (1991)
    Springer
    Euphytica 55 (1991), S. 277-285 
    Details
    ISSN: 1573-5060
    Keywords: HMW glutenin subunits ; bread-making quality ; Triticum aestivum ; wheat ; epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relation has been studied between the high molecular weight glutenin (HMWg) subunit alleles and the bread-making quality of 226 lines of winter wheat (T. aestivum L.), grown in The Netherlands. The lines represented a wide range of genetic backgrounds, and had not been selected for quality, in contrast to the established varieties used by other authors. The variation in HMWg subunit genotypes accounted for about 20% of the total variation in loaf volume among the lines. Most important was the allelic variation at the Glu-D1 locus. The Glu-D1 allele encoding the subunits 5+10 was superior to its allelic counterpart, encoding 2+12. The difference in average of loaf volume between groups of lines containing 5+10 or 2+12 was negatively related with protein content of the flours. When protein content was below 9.2%, no effect of allelic variation at the Glu-D1 locus was present. Epistatic effects between the Glu-I loci also contributed to the variation in loaf volume of the lines: i.e. the effect of allelic variation at Glu-A1 and Glu-B1 depended on the allele present at the Glu-D1. The contribution of the epistatic effects was about half the contribution of the additive effects, and should therefore be included in predictive models for bread-making quality.
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    URL: http://dx.doi.org/10.1007/BF00021248
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    The ‘European Apple Genome Mapping Project’-developing a strategy for mapping genes coding for agronomic characters in tree species (1991)
    Springer
    Euphytica 56 (1991), S. 89-94 
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    ISSN: 1573-5060
    Keywords: Malus ; apple ; genetic mapping ; isozymes ; DNA techniques ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A recently initiated collaborative project involving apple breeders in seven European countries is described. The objective is to improve the European apple crop by molecular-aided breeding to increase efficency and reduce the time-scale in breeding for resistance, tree habit and fruit quality. The strategy adopted provides a model for similar studies in fruit, forest and other woody species. The project is based on progenies from a small number of crosses involving many important agronomic genes. Replication of these reference progenies by vegetative propagation will enable studies to be carried out simultaneously in each country. By developing a range of molecular markers, including isozymes, RFLPs and sequence-tagged DNA probes, an integrated molecular map is being constructed for use in a wide range of breeding and genetic studies. Construction of a database recording many mapped molecular markers will enable efficient exploitation of data in future genetic, breeding and physiological studies of apple. Aspects of the adopted strategy, techniques and management are discussed in the context of mapping genes in perennial crop genomes.
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    Wheats with purple and blue grains: a review (1991)
    Springer
    Euphytica 56 (1991), S. 243-258 
    Details
    ISSN: 1573-5060
    Keywords: Triticum species ; wheat ; grain colour ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In addition to white and red grains, wheats with purple and blue grains may occur. Purple grain colour is caused by anthocyanins in the pericarp whereas blue colour is caused by anthocyanins in the aleurone layer. Purple grains occur in tetraploid wheats from Ethiopia, and in one bread wheat accession apparently native to China. Although the use of the purple and blue grain characters as markers has been suggested, their expression is often erratic, especially when heterozygous. No hexaploid wheat with blue grains was described prior to the artificial introgression of genes from diploid wheat and Agropyron species. The number of different sources of blue aleurone gene(s) from Agropyron elongatum is unknown. It is possible that with exchange between researchers the same or related accessions have been used at several research stations. Accessions of diploid wheats are known to possess blue aleurone. The breeding history of a number of purple and blue grained accessions is described. Research should indicate the source species of the gene for blue aleurone of the blue-grained Barevna.
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    Genes for leaf rust resistance in Indian and Pakistani wheats tested with Mexican pathotypes of Puccinia recondita f.sp. tritici (1991)
    Springer
    Euphytica 57 (1991), S. 27-36 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita tritici ; leaf rust ; rust resistance ; slow rusting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genes conferring low seedling reaction to Mexican pathotypes of Puccinia recondita f. sp. tritici in 71 bread wheat (Triticum aestivum L.) cultivars from India and Pakistan were postulated. In total, 9 known and one unknown genes were identified, either singly or in combination: Lr1 (in 20 cultivars), Lr3 (5), Lr10 (21), Lr11 (1), Lr13 (43), Lr17 (5), Lr23 (14), Lr26 (2), Lr27 + Lr31 (2), and the unknown gene in 2 cultivars. Additional temperature-sensitive seedling resistance appeared to occur in 27 cultivars. This resistance in at least 15 cultivars appeared to be due to Lr34. Area under the disease progress curve (AUDPC) for these 27 cultivars indicated variable levels of adult plant resistance. Several other cultivars with high seedling infection types to one or more of the predominant field pathotypes were also partially resistant in the field. High levels of adult plant resistance occurred in some cultivars even in the absence of known seedling resistance genes with major effects.
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    URL: http://dx.doi.org/10.1007/BF00040475
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    Influence of race and post infection temperature on two components of partial resistance to wheat leaf rust in seedlings of wheat (1991)
    Springer
    Euphytica 58 (1991), S. 13-20 
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    ISSN: 1573-5060
    Keywords: Adult plant resistance ; infection frequency ; latency period ; partial resistance ; Puccinia recondita f.sp. tritici ; race-specificity ; temperature sensitivity ; Triticum aestivum ; wheat ; wheat leaf rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Components of partial resistance, infection frequency and latency period, were determined in 71 winter and spring wheat genotypes in the seedling stage, after infection with three races of leaf rust (‘Felix 3B’, ‘Clement B’ and ‘Betuwe 85C’) at three different day/night temperature regimes (24/18°C, 18/12°C and 12/6°C). The genotypes were split into two groups and two separate experiments were carried out. Five genotypes, SVP 84039, Akabozu, Banco, BH 1146 and Orso, conferred a low infection frequency and a long latency period and Westphal 12A a long latency period, indicating a relatively high level of partial resistance. The correlation coefficient between infection frequency and latency period was low. Race-specificity was not found. There was a significant temperature effect on the latency period. In the second experiment the temperature x genotype interaction was significant. Temperature-response functions of transformed data demonstrated that the latency periods of four relatively resistant genotypes, Westphal 12A, Banco, BH 1146 and Orso and of Sarno and Mirela were most sensitive to temperature. The range between the genotypes with the longest and the shortest latency period was highest at 12°C. Therefore, low temperature regimes are preferred to distinguish differences in level of partial resistance.
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    An investigation of the relationship between the F1 potential and the measures of genetic distance among wheat lines (1991)
    Springer
    Euphytica 58 (1991), S. 165-170 
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    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; choice of parents ; distance analysis ; standardized potence ; character selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three groups of characters selected using three methods were used to calculate genetic distance among two groups of winter wheat lines using Euclidean distance based on principal components. These wheat lines were then clustered by the unweighted pair-group arithmetic average method. The relationship between genetic distance and standardized potence was seemingly influenced by genetic materials. In order to obtain promising crosses, it is advisable to select parents from intercluster rather than intracluster materials. The results suggested that the repeatability method was better than the conventional and weighting methods for selecting characters for distance analysis.
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    Inhibition of tillering in cereals by substituted 2-phenoxypropionic acids (1991)
    Springer
    Plant growth regulation 10 (1991), S. 205-214 
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    ISSN: 1573-5087
    Keywords: tillering ; wheat ; barley ; rice ; 2-phenoxypropionic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The properties of various mono- and di-substituted analogues of 2-phenoxypropionic acid as inhibitors of tillering were investigated on wheat, barley and rice. Highest levels of activity were shown by (R)2-Cl,5-Cl, (R)2-Cl,5-F, and (R)2-Cl,5-methyl analogues. Few or no signs of phytotoxic effects (leaf chlorosis or necrosis) were evident on wheat or barley following spray application of these compounds. Rice was both more susceptible to inhibition of tillering and phytotoxic effects. However, almost complete inhibition of tillering was achieved by application of some compounds to rice with little or no phytotoxicity. Comparisons were made between the properties of these compounds and commercially used phenoxyacetic and phenoxypropionic herbicides and plant growth regulators. Dichlorprop inhibited tillering in rice, fenoprop in wheat and rice, and fluroxypyr in wheat, all without phytotoxic effects.
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    Wheat anther culture: effect of genotype and environmental conditions (1991)
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    Plant cell, tissue and organ culture 24 (1991), S. 233-236 
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    ISSN: 1573-5044
    Keywords: anther culture ; cultivars ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-two cultivars and lines of winter and spring wheat (Triticum aestivum L.) were studied, most for the first time, for their anther culture response. The response was genotype dependent. Plants grown in the field gave higher callus induction frequency than those grown in the greenhouse and the controlled environment chamber. Donor plants grown in a season of low drought stress as compared to a season of severe drought stress resulted in a higher frequency of callus induction. Spherical microcalli were observed in two wheat genotypes in some of only those anthers that were placed with only one loculus in contact with the medium. Wheat lines that were more responsive to anther culture were identified.
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    Adherence of Agrobacterium tumefaciens to the cells of immature wheat embryos (1991)
    Springer
    Plant cell, tissue and organ culture 25 (1991), S. 199-208 
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    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; cocultivation ; enzymatic digestion ; tissue culture ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agrobacterium attached to wheat embryos in vitro. This attachment was plasmid independent, and occurred on both wounded and unwounded cell surfaces. The pattern of attachment clearly demonstrated that bacterial attachment to cereal cells follows the same trends observed for dicotyledonous plants. During the inoculation period the bacterial cells attach to the plant cell walls either with lateral or polar orientation. Wounding (mechanical or enzymatic) preferentially promoted adherence of the bacteria at the wound site, however, attachment was not wound dependent.
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    Agrobacterium tumefaciens-gene transfer into wheat tissues (1991)
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    Plant cell, tissue and organ culture 25 (1991), S. 209-218 
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    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; cocultivation ; tissue culture ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA can be transferred by Agrobacterium tumefaciens to wheat, albeit at very low frequencies. Transfer of agrobacterial DNA occurred in cultures where the embryos had been subjected to partial enzymatic digestion prior to cocultivation with the bacteria. It is unclear whether this is by the normal process mediated by the Ti virulence genes and the border repeats of the T-DNA. The Southern hybridization patterns indicate that in one cell line the T-DNA had undergone extensive rearrangements, and might indicate that the process of T-DNA transfer and integration might differ in the case of cereals. This could suggest the method of transfer and ultimately the expression of these genes in cereal cells may be different to that observed in other monocotyledonous and dicotyledonous species.
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    Phosphate sorption approach for determining phosphorus requirements of wheat in calcareous soils (1991)
    Springer
    Nutrient cycling in agroecosystems 28 (1991), S. 67-72 
    Details
    ISSN: 1573-0867
    Keywords: Phosphate adsorption ; external P requirement ; wheat ; fertilizer requirements ; calcareous soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five field experiments involving P application rates from 0 to 66 kg P ha−1 were conducted on irrigated wheat at Tandojam, Pakistan. The soils belonged to two great soil groups, Torrifluvent and Camborthid. All soils were calcareous. Olsen-P contents ranged from 3.5 to 6.3 mg P kg−1. Phosphate sorption curves were developed for soils from control (no P) plots at each site. Concentrations of P in solution established by fertilization in the field as estimated from the sorption curves ranged from 0.008 to 0.16mg P L−1. Actual grain yields were converted to relative grain yields and plotted against corresponding concentrations of P in solution. Yield response to P application was obtained in each experiment. Control plot yields ranged from 57 to 89% of maximum yield of respective experiments. Phosphorus requirements of wheat were 0.032 mg L−1 for 95% yield as determined from a composite yield response curve. Predicted quantities of P required to attain 0.032 mg P L−1 ranged from 18 to 29 kg P ha−1. The results of the study suggest that the P sorption approach can be used as a rational basis for making P fertilizer recommendations for various soil-crop combinations.
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    Selenium content of wheat as affected by selenate and selenite contained in a Cl- or SO4-based NPK fertilizer (1991)
    Springer
    Nutrient cycling in agroecosystems 30 (1991), S. 1-7 
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    ISSN: 1573-0867
    Keywords: Chloride ; pH ; residual effect ; sandy soil ; selenate ; selenite ; sulphate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In areas where soils supply inadequate Se to crops to meet animal nutritional needs, it may be necessary to supplement Se in some fashion. This greenhouse experiment was designed to study the effect of selenate and selenite, incorporated with a Cl- or a SO4-based NPK (16-5-12) fertilizer added to a low-Se soil, on the Se concentration in wheat (Triticum aestivum L.). The sandy soil was limed to pH 5.5 and 6.5 prior to receiving 0, 0.03, or 0.06 mg Se kg−1 of soil with each NPK source each year for two years. The SO4-based NPK fertilizer treatment was used only at the 0.06 mg Se kg−1 rate. In the third year, the residual effect of the Se applied in the first 2 years was studied. The grain yield among treatments differed significantly but the effects of the rate or source were not consistent in all the years. However, significantly higher yields were obtained at pH 6.5 than at pH 5.5. Selenium concentration and uptake in wheat grain increased in proportion to the applied selenate but not selenite. However, selenite increased the Se concentration and uptake over the control. The concentration and uptake of Se were higher from selenate than from selenite. The effect of the Cl- and SO4-based NPK fertilizers was different on selenate and selenite. Selenium accumulation in wheat grain was greater from the Cl-based NPK fertilizer when selenate was used but it was greater from the SO4-based NPK fertilizer when selenite was used. The Se concentration in wheat, especially from selenate, was generally higher at the higher pH level except in the first year where it was higher at the lower pH level. The uptake of Se from the Se-enriched NPK fertilizers was generally as high as from the Se applied separately. The residual effect of both sources of Se applied in the first 2 years was very poor in the third year. The results suggest that the Se concentration in wheat grain can be increased by incorporating the Se salts in the NPK fertilizers but more selenate is recovered than selenite. Selenate should, however, be incorporated in a Cl-based NPK fertilizer to avoid the ionic competition from SO4 ions in the SO4-based NPK fertilizer. The uptake of Se is generally higher at pH 6.5 than at pH 5.5.
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    Effects of a long chain aliphatic alcohol mixture on growth and solute accumulation in water stressed wheat seedlings under laboratory conditions (1991)
    Springer
    Plant growth regulation 10 (1991), S. 223-234 
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    ISSN: 1573-5087
    Keywords: moisture stress ; polyethylene glycol ; long-chain aliphatic alcohols ; seedling growth, solute accumulation ; hydrolases ; wheat ; Triticum aestivum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Polyethylene glycol (PEG)-induced water stress adversely affected the germination and seedling growth of Sonalika and WL2265 cultivars of wheat (Triticum aestivum L.). Application of a mixture of long-chain aliphatic alcohols having the composition C-24 Tetracosanol (10%), C-26 Hexacosanol (16%), C-28 Octacosanol (15%), C-30 Tricontanol (30%), C-32 Dotriacontanol (15%) and C-34 Tetratriacontanol (14%), partially ameliorated these effects and promoted both percent germination and seedling growth. Application also stimulated the activities of the hydrolases α- and β-amylase and acid invertase, so increasing free sugar accumulation. A role for long chain aliphatic alcohols in the regulation of carbohydrate metabolism is suggested. The alleviation of moisture stress by application of this mixture suggest that long chain aliphatic alcohols may be most effective at low water potentials.
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    Characterization of stem rust resistant derivatives of wheat cultivar Amigo (1991)
    Springer
    Euphytica 58 (1991), S. 245-252 
    Details
    ISSN: 1573-5060
    Keywords: Introgression ; leaf rust ; Puccinia spp. ; rust resistance ; species-specific probes ; stem rust ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Originally developed for resistance to greenbug derived from Insave rye, Amigo wheat carries two genes for resistance to stem rust. One of these genes is associated with a rye chromosome 1RS segment carrying the Sec-1 protein marker and presumably greenbug resistance. The second gene which is genetically linked to leaf rust resistance is associated with an Agropyron-derived segment. Rust tests in Canada confirmed that these genes were Sr24 and Lr24. In contrast to Agent and certain 3D/Ag derivatives from Dr. E.R. Sears, the Amigo source of Sr24/Lr24 freely recombined with white seed colour during backcrossing.
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    Transport of Rb and Sr to the ear in mature, excised shoots of wheat: Effects of temperature and stem length on Rb removal from the xylem (1991)
    Springer
    Plant and soil 132 (1991), S. 281-288 
    Details
    ISSN: 1573-5036
    Keywords: internode ; phloem ; rubidium ; solute transfer ; strontium ; temperature ; Triticum aestivum L. ; wheat ; xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (Triticum aestivum L. cv. ‘Arina’) shoots grown in the field were excised post-anthesis and incubated in the laboratory for 72 h standing in 2 mM RbCl+2 mM SrCl2. Strontium is a phloemimmobile, xylem-mobile element and indicates the distribution of the xylem sap in the plant. Rubidium is easily transported in the phloem and behaves similarly to the highly mobile K as far as the redistribution within the plant is concerned, although Rb cannot substitute physiologically or biochemically for K. The Sr contents in the ear were hardly affected by stem length or by steam-girdling (phloem-interruption). Rubidium on the other hand accumulated in the stem. A peduncle length of 5 cm was sufficient to decrease the Rb concentration in the xylem by more than 50% at 25°C. Only a minor quantity of Rb reached the ear after passing through 20 cm of stem without nodes and this transport was prevented by steam-girdling. A remarkable flux of Rb into the ear was observed in shoots with a vascular connection between the flag leaf lamina and the ear. Our results suggest that Sr was transported with the transpiration stream, while Rb was rapidly eliminated from the xylem and reached the ear via the phloem. The temperature optimum for the removal of Rb from the xylem was around 35°C. The nodes may further contribute, but are not prerequisites for this redistribution. The observed transfer processes could allow a solute specific transport via the xylem and phloem of maturing cereals and may be an important factor influencing the nutrient economy in the field.
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    Tolerance to high concentrations of boron for the amphiploid of Triticum aestivum × Agropyron elongatum (1991)
    Springer
    Plant and soil 133 (1991), S. 297-299 
    Details
    ISSN: 1573-5036
    Keywords: Agropyron ; amphiploid ; boron toxicity ; tolerance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The response to high concentrations of B in soil was compared for Triticum aestivum L. (vars. Chinese Spring and Halberd) and the amphiploid of Chinese Spring × Agropyron elongatum (Host). The tolerance of the three genotypes, with respect to yield at the high B treatments, was amphipolid〉Halberd〉Chinese Spring. The concentration of B in whole shoots was similar for the amphiploid and Halberd and significantly higher for Chinese Spring. The mechanism for tolerance to B appears to be the same for the amphiploid and wheat and is related to reduced accumulation of B in shoots.
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    In vitro techniques for selecting wheat (Triticum aestivum L.) for Fusarium-resistance. I. Double-layer culture technique (1991)
    Springer
    Euphytica 57 (1991), S. 251-257 
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    ISSN: 1573-5060
    Keywords: Fusarium resistance ; Fusarium spp. ; wheat ; Triticum aestivum ; double-layer technique ; in vitro selection ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Calluses of spring and winter wheats (Triticum aestivum L.) were selected for Fusarium resistance in vitro, using the double-layer culture technique. Potato-dextrose agar medium in vials was inoculated with mycelia of Fusarium graminearum and F. culmorum. After one week, fungal cells were killed by autoclaving and the agar medium containing the thermostable toxic metabolites was overlayered with MS callus-growing medium. Later, wheat calluses were placed on the upper medium for 4–5 weeks, and from the surviving calluses plants were regenerated. R2 seedling populations from self-fertilized R1 plants of 4 varieties were tested for Fusarium resistance by artificial infections in the greenhouse, and 3% of the regenerated R2 plants have been found to be more resistant than the original cultivars.
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    Production and cytogenetic analysis of the intergeneric hybrids between nine Elymus species and common wheat (Triticum aestivum L.) (1991)
    Springer
    Euphytica 58 (1991), S. 81-95 
    Details
    ISSN: 1573-5060
    Keywords: Elymus species ; genome ; germplasm resources ; intergeneric hybridization ; meiosis ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Intergeneric hybridizations were performed between Triticum aestivum L. (2n=6x=42, AABBDD) and twelve Elymus species, ten tetraploids (2n=4x=28, SSYY) and two hexaploids (2n=6x=42, SSHHYY and SSYY??), namely, E. pendulinus (Nevski) Tzvelev, E. altissimus (Keng), Löve, E. anthosachnoides (Keng) Löve, E. brevipes (Keng) Löve, E. dolichatherus (Keng) Löve, E. parviglumis (Keng) Löve, E. pseudonutans (Keng) Löve, E. tibeticus (Melderis) G. Singh, E. semicostatus (Nees ex Steud.) Melderis, E. caucasicus (C. Koch) Tzvelev (tetraploids), and E. tsukushiensis Honda and E. tschimganicus (Drobov) Tzvelev (hexaploids). The hybrid seed sets in different combinations varied from 4.8%–72.2%, and embryo yields ranged from 3.2%–36.7%. Nine combinations produced adult hybrid plants, eight pentaploids and one hexaploid, through embryo rescue. The production of hybrid plants varied from 1.2%–30%. Chromosome pairing was analysed at metaphase I in the parental species and the hybrids. Average meiotic configurations of 33.51 I+0.74 II+0.03 III+0.001 IV were observed amongst the pentaploid hybrids although there was a large variation, and 31.42 I+5.21 II+0.04 III was recorded in the hexaploid hybrid. It is concluded from the study that: 1) very little pairing occurred between the ABD genomes in T. aestivum and the different genomes in the tetraploid Elymus species. even though the pairing behaviour varied in different combinations; 2) the hexaploid combination had distinctly higher chromosome pairing than the other pentaploid combinations, which could be due either to a low degree of homoeology between the T. aestivum and E. tschimganicus genomes or that the latter species contains, certain gene(s) which increase the meiotic pairing between genomes from the same parental species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035343
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  • 81
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    Zinc deficiency and the formation of white structures in immature embryo cultures of wheat (Triticum aestivum L.) (1991)
    Springer
    Plant cell, tissue and organ culture 24 (1991), S. 9-12 
    Details
    ISSN: 1573-5044
    Keywords: culture medium ; embryo culture ; microelements ; wheat ; zinc ; zinc deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of microelements on the induction of embryogenic callus from epiblast and scutellum of wheat (Triticum aestivum L.) embryos was studied by the sequential omission of each of the microelements from Murashige & Skoog medium. Omission of iron caused a marked decrease in yield and poor shoot formation from embryogenic callus. The yield of embryogenic callus on medium without added manganese was also reduced. Omission of boron, copper-cobalt, iodine, and molybdenum had little effect on the induction of embryogenic epiblast callus. By contrast there was a marked increase in the formation of white structures on the medium without any microelements or, specifically without addition of zinc. Since the formation of typical embryoids of wheat is associated with the formation of white structures, our result highlights the importance of certain microelements on somatic embryogenesis of wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044258
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  • 82
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    TheMspI restriction fragment length polymorphism of human aldolase B gene on chromosome 9q21.3–q22.2 (1991)
    Springer
    Journal of human genetics 36 (1991), S. 325-329 
    Details
    ISSN: 1435-232X
    Keywords: genetic linkage map ; RFLP ; human aldolase B ; PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The probe containing the exon 9 of human aldolase B gene revealedMspI polymorphism involving two fragments 6.5 and 3.0 kb long with the high frequency of heterozygosity (21%). The two alleles can be distinguished efficiently by the DNA PCR-restriction fragment length polymorphism procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01883606
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  • 83
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    In vitro production of pycnidia by Septoria tritici (1991)
    Springer
    European journal of plant pathology 97 (1991), S. 65-72 
    Details
    ISSN: 1573-8469
    Keywords: speckled leaf blotch ; wheat ; growth type ; pseudopycnidia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The in vitro production of pycnidia bySeptoria tritici was examined on six media reported to induce the formation of fruiting bodies. Among 26 freshly isolated cultures from various parts of the world, consistent differences in growth type were found which were only partially influenced by nutritional and environmental conditions. Cultures with yeast-like growth produced hardly any pycnidia or pseudopycnidia, while cultures with intermediate or mycelial growth types produced them frequently. Incubation in continuous darkness induced intermediate to mycelial growth types rather than yeast-like growth types in some cultures, and concomitantly the production of more pycnidia. Potato-dextrose agar induced intermediate to mycelial growth types and production of (pseudo)pycnidia more often than V8 agar and wheat leaf extract agar, which had previously been reported to be especially beneficial to (pseudo) pycnidium formation byS. tritici. Isolates with a consistently yeast-like growth type, producing (virtually) no fructifications under any of the experimental conditions, were slightly stimulated to form pseudopycnidia on water agar supplemented with sterile pieces of maize, wheat or carnation leaves.
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    URL: http://dx.doi.org/10.1007/BF01974270
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  • 84
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    La localisation, le comportement et les relations «Proie-prédateur» chezCoccinella septempunctata dans un champ de blé (1991)
    Springer
    BioControl 36 (1991), S. 213-225 
    Details
    ISSN: 1573-8248
    Keywords: Coccinella septempunctata ; distribution verticale ; comportement ; alimentation ; puceron ; blé ; Coccinella septempunctata ; vertical distribution ; behaviour ; feeding ; aphid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary Observations were made in a wheat field during daylight hours to determine how the vertical distribution of adults and larvae ofCoccinella septempunctata changed with season and in response to aphid populations on which this coccinellid feeds. In early spring, this thermophilic coccinellid (larvae and adults) remained close to the ground and on the lower part of tiller leaves where it was highly active. Adults frequently groomed their head appendages. By June, most adults and larvae were found on the upper parts of tillers, the area preferred by their aphid prey. The beetles remained close to such sites and hence their vertical distribution corresponded to that of the aphids on which they fed. The fact that the beetles appeared to spend most of their time resting and moving within wheat crops, and little time feeding, raises the question of how the larvae and adults satisfy their dietary needs.
    Notes: Abstract Des adultes et des larves deC. septempunctata ont été observés individuellement dans un champ de blé afin de préciser, au cours de la journée et en fonction de la saison, leur distribution sur le végétal, la durée des principales séquences de leur comportement et leurs relations avec les pucerons. Au début du printemps, cette espèce thermophile est présente sur le sol et sur les feuilles basses des talles où elle est très mobile. Les adultes font très fréquemment la toilette des appendices de la tête. En juin, la population larvaire et imaginale se regroupe sur la partie supérieure des talles où se trouvent les pucerons. A cette époque, l'immobilité domine. Ces variations de la distribution verticale sont confirmées par la position sur le végétal des proies capturées. Les données suggèrent que, dans les champs, cette coccinelle consacre peu de temps à son alimentation. Le faible nombre de proies ingérées pose le problème de la couverture de ses besoins trophiques.
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    URL: http://dx.doi.org/10.1007/BF02374557
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