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  • 1
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Glycine max (L.) Merr.] have been developed over the past 10 yr. These maps are primarily based on restriction fragment length polymorphism (RFLP) markers. Parental surveys have shown that most RFLP loci have only two known alleles. However, because the soybean is an ancient polyploid, RFLP probes typically hybridize and map to more than one position in the genome. Thus, the polymorphic potential of an RFLP probe is primarily a function of the frequency of the two alleles at each locus the probe detects. In contrast, simple sequence repeat (SSR) markers are single locus markers with multiple alleles. The polymorphic potential of an SSR marker is dependent on the number of alleles and their frequencies. Single locus markers provide an unambiguous means of defining linkage group homology across mapping populations. The objective of the work reported here was to develop and map a large set of SSR markers. A total of 606 SSR loci were mapped in one or more of three populations: the USDA/Iowa State G. max × G. soja F2 population, the Univ. of Utah Minsoy × Noir 1 recombinant inbred population, and the Univ. of Nebraska Clark × Harosoy F2 population. Each SSR mapped to a single locus in the genome, with a map order that was essentially identical in all three populations. Many SSR loci were segregating in two or all three populations. Thus, it was relatively simple to align the 20+ linkage groups derived from each of the three populations into a consensus set of 20 homologous linkage groups presumed to correspond to the 20 pairs of soybean chromosomes. On the basis of in situ segregation or linkage reports in the literature all but one of the classical linkage groups can now be assigned to a corresponding molecular linkage group.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Irrigation science 10 (1989), S. 99-111 
    ISSN: 1432-1319
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soybean [Glycine max (L.) Merr.] producers in the Great Plains region usually employ either a seasonal soil water balance approach, or a growth stage sensitivity approach, relative to scheduling sprinkler irrigation events. We conducted an empirical evaluation of the response of six soybean cultivars to three irrigation strategies. One was an irrigation scheduling (IS) system based solely on maintaining a soil water content in the root zone between 50% and 80% of the total plant available soil water capacity. The other two strategies involved the same depletion criterion for triggering irrigation events, except that the first irrigation was intentionally delayed until the flowering (FL) stage, or the mid-pod elongation (PD) stage. The total water amount applied during each season was approximately similar for the IS, FL, and PD strategies. Thus, the primary difference among the three strategies was the time frame during which irrigation events were scheduled. In the 1983 test, the yields attained in the IS, FL, and PD treatments were not significantly different from each other (i.e. 4.08, 4.08, and 4.04 Mg/ha, respectively), and were nearly double the yield obtained in the nonirrigated (NI) check treatment (2.29 Mg/ha). In the 1984 test, the yields of the IS, FL, and PD treatments were again not significantly different (2.02, 2.05, and 2.22 Mg/ha, respectively). However, the 1984 yield response to irrigation was also not significant relative to the NI check (1.90 Mg/ha), primarily because of low plant populations and a shorter growing season. Thus, this two-year experiment indicated that delaying irrigation until the FL or the PD stages of soybean reproductive development could be just as effective (i.e. 1983 data), or at least no more ineffective (i.e. 1984 data), in enhancing soybean yield compared to the IS strategy (Fig. 1). The soil water balance and soybean growth stage sensitivity approaches, when combined, could thus constitute an effective strategy of soybean sprinkler irrigation management in the Great Plains region.
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  • 3
    ISSN: 1432-2242
    Keywords: Glycine max ; Chloroplast DNA ; RFLP ; Population ; Male-sterile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphisms (RFLPs) were used to assess chloroplast DNA (cpDNA) variation in a population of soybeans subjected to continuous cycles of forced outcrossing. This population was derived by crossing 39 female lines with four male-sterile (Ms2ms2) maintainer lines and advancing each generation by selecting only outcrossed seed borne on male-sterile (ms2ms2) plants. Analysis of the original 39 female lines revealed three groups based on cpDNA RFLPs. These three groups had been previously documented in soybeans, and the distribution of these groups among the female parents of this population was similar to that observed in germ plasm surveys of soybean. Thirty-four of the female parents had group I cpDNA, 3 had group II, and 2 had group III. Plants collected from this population after seven cycles of outcrossing were scored for four morphological traits (flower color, pubescence color, seed color, and pubescence type) known to be controlled by alleles at single nuclear loci. The frequencies of the phenotypes observed in this study indicated that the population underwent random mating with respect to flower and pubescence color, but deviated from random mating at the other two loci. Analysis of 158 of these same plants collected from the population after seven cycles of outcrossing revealed no individuals with group II or group III cpDNAs. The fixation of the group I cpDNA marker in this outcrossing population was judged to result primarily from selection against individuals in the population with the rare cpDNAs.
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  • 4
    ISSN: 1432-2242
    Keywords: Glycine max ; Near-isogenic lines ; Molecular markers ; RFLP ; Linkage ; Genetic map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage between that molecular marker and the introgressed marker. In this study, we examined the utility of RFLPs as molecular markers for the NIL genemapping approach. The allelic status of fifteen RFLP loci was determined in 116 soybean RP/NIL/DP line sets; 66 of the ‘Clark’ RP type and 50 of the ‘Harosoy’ RP type. Of the 1740 possible allelic comparisons (116 NILs x 15 RFLP loci), 1638 were tested and 462 (33.9%) of those were informative (i.e., the RP and DP had different RFLP alleles). In 15 (3.2%) of these 462 cases the NIL possessed the DP-derived RFLP allele, leading to a presumption of linkage between the RFLP locus and the introgressed conventional marker locus. Two presumptive linkages, pK-3 — and pK-472 — Lf i, were subsequently confirmed by cosegregation linkage analysis. Although not yet confirmed, two other associations, pk-7 ab and pK-229 — y 9 seemed to be plausible linkages, primarily because the pk-7 — ab association was detected in two independently derived NILs and both markers of the pK-229 — y 9 association were known to be linked to Pb. The data obtained in this investigation indicated that RFLP loci were useful molecular markers for the NIL gene-mapping technique.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 96 (1998), S. 1064-1068 
    ISSN: 1432-2242
    Keywords: Key words Glycine max ; Genome size ; Seed size ; Leaf size ; Flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A correlation between genome size and agronomically important traits has been observed in many plant species. The goal of the present research was to determine the relationship between genome size, seed size, and leaf width and length in soybean [Glycine max (L.) Merr.] Twelve soybean strains, representing three distinct seed size groups, were analyzed. Flow cytometry was used to estimate their 2C nuclear DNA contents. Data on seed size and leaf size of the 12 strains were obtained from 1994 and 1995 field experiments. Variation of 2C nuclear DNA among the 12 soybean strains was 4.6%, ranging from 2.37 pg for a small-seed strain to 2.48 pg for a large-seed strain. Strain seed size was positively associated with leaf width (r=0.92) and leaf length (r=0.93). Genome size was highly correlated with seed size (r=0.97), leaf width (r=0.90) , and leaf length (r=0.93). The results of our study indicate that there is a significant correlation between genome size and leaf and seed size in soybean. It is possible that selection for greater seed size either leads to, or results from, greater genome size. If so, this relationship might be worth exploring at a more fundamental level.
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  • 6
    Publication Date: 1992-03-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 7
    Publication Date: 1992-03-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 8
    Publication Date: 1999-07-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 9
    Publication Date: 1980-09-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
    Publication Date: 1983-05-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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