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  • 1
    Electronic Resource
    Electronic Resource
    Structural studies of Bacillus subtilis glutamine synthetase - Further purification, sulfhydryl groups, and the NH"2-terminal amino acid sequence
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 178 (1977), S. 644-651 
    Details
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-9861(77)90236-3
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  • 2
    Electronic Resource
    Electronic Resource
    Interaction of a radiolabeled cytokinin photoaffinity probe with a receptor protein
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 96 (1980), S. 1325-1334 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(80)90096-0
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation of two neuropeptides in the AKH/RPCH-family from horseflies (diptera)
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 151 (1988), S. 656-663 
    Details
    ISSN: 0006-291X
    Keywords: AKH,; adipokinetic hormone ; CA,; corpora allata ; CC,; corpora cardiaca ; DCCI,; Dipteran corpora cardiaca factor I ; DCCII,; Dipteran corpora cardiaca factor II ; HPLC,; high performance liquid chromatography ; Hz-AKH,; Heliothis zea AKH ; PITC,; phenylisothiocyanate ; PTC,; phenylthiocarbamyl ; RPCH,; red pigment concentrating hormone ; SIR,; standard integrated response ; TEAP,; triethylammonium phosphate ; TFA,; trifluoroacetic acid ; UV,; ultraviolet
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(88)80331-0
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of two neuropeptides in the AKH/RPCH-family from horseflies (diptera)
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 151 (1988), S. 656-663 
    Details
    ISSN: 0006-291X
    Keywords: AKH,; adipokinetic hormone ; CA,; corpora allata ; CC,; corpora cardiaca ; DCCI,; Dipteran corpora cardiaca factor I ; DCCII,; Dipteran corpora cardiaca factor II ; HPLC,; high performance liquid chromatography ; Hz-AKH,; Heliothis zea AKH ; PITC,; phenylisothiocyanate ; PTC,; phenylthiocarbamyl ; RPCH,; red pigment concentrating hormone ; SIR,; standard integrated response ; TEAP,; triethylammonium phosphate ; TFA,; trifluoroacetic acid ; UV,; ultraviolet
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(88)80331-0
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  • 5
    Electronic Resource
    Electronic Resource
    The RecE recombination pathway mediates recombination between partially homologous DNA sequences: Structural analysis of recombination products
    Amsterdam : Elsevier
    Journal of Structural Biology 104 (1990), S. 97-106 
    Details
    ISSN: 1047-8477
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/1047-8477(90)90063-I
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization and application of soybean YACs to molecular cytogenetics (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 483-488 
    Details
    ISSN: 1617-4623
    Keywords: Degenerate oligonucleotide-primed PCR (DOP-PCR) ; Fluorescence in situ hybridization ; Glycine max ; Yeast artificial chromosomes ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Yeast artificial chromosomes (YACs) are widely used in the physical analysis of complex genomes. In addition to their value in chromosome walking for map-based cloning, YACs represent excellent probes for chromosome mapping using fluorescence in situ hybridization (FISH). We have screened such a library for low-copy-number clones by hybridization to total genomic DNA. Four clones were chosen for chromosome tagging based upon their low or moderate signal. By using degenerate oligonucleotide-primed PCR (DOP-PCR), we were able to use relatively small amounts of soybean YAC DNA, isolated directly by preparative pulsed-field gel electrophoresis, as FISH probes for both metaphase chromosome spreads and interphase nuclei. FISH chromosomal analysis using the three of the clones as probes resulted in relatively simple hybridization patterns consistent with a single homologous locus or two homoeologous loci. The fourth YAC probe resulted in a diffuse hybridization pattern with signal on all metaphase chromosomes. We conclude that YACs represent a valuable source of probes for chromosomal analysis in soybean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173014
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  • 7
    Electronic Resource
    Electronic Resource
    Characterization and application of soybean YACs to molecular cytogenetics (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 483-488 
    Details
    ISSN: 1617-4623
    Keywords: Key words Degenerate oligonucleotide-primed PCR(DOP-PCR) ; Fluorescence in situ hybridization ; Glycine max ; Yeast artificial chromosomes ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Yeast artificial chromosomes (YACs) are widely used in the physical analysis of complex genomes. In addition to their value in chromosome walking for map-based cloning, YACs represent excellent probes for chromosome mapping using fluorescence in situ hybridization (FISH). We have screened such a library for low-copy-number clones by hybridization to total genomic DNA. Four clones were chosen for chromosome tagging based upon their low or moderate signal. By using degenerate oligonucleotide-primed PCR (DOP-PCR), we were able to use relatively small amounts of soybean YAC DNA, isolated directly by preparative pulsed-field gel electrophoresis, as FISH probes for both metaphase chromosome spreads and interphase nuclei. FISH chromosomal analysis using the three of the clones as probes resulted in relatively simple hybridization patterns consistent with a single homologous locus or two homoeologous loci. The fourth YAC probe resulted in a diffuse hybridization pattern with signal on all metaphase chromosomes. We conclude that YACs represent a valuable source of probes for chromosomal analysis in soybean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173014
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  • 8
    Electronic Resource
    Electronic Resource
    Ribosomal RNA genes in soybean and common bean: chromosomal organization, expression, and evolution (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 136-141 
    Details
    ISSN: 1432-2242
    Keywords: Key words Fluorescence in situ hybridization ; Glycine max ; Phaseolis vulgaris ; Nucleolus ; rDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ribosomal RNA (5S and 45S) genes were investigated by FISH in two related legumes: soybean [Glycine max (L.) Merr.] and common bean (Phaseolis vulgaris L.). These species are both members of the same tribe (Phaseoleae), but common bean is diploid while soybean is a tetraploid which has undergone diploidization. In contrast to ploidy expectations, soybean had only one 5S and one 45S rDNA locus whereas common bean had more than two 5S rDNA loci and two 45S rDNA loci. Double hybridization experiments with differentially labelled probes indicated that the soybean 45S and 5S rDNA loci are located on different chromosomes and in their distal regions. Likewise, the common bean 45S and 5S rDNA loci were on unique chromosomes, though two of the 5S rDNA loci were on the same chromosome. FISH analysis of interphase nuclei revealed the spatial arrangement of rDNA loci and suggested expression patterns. In both species, we observed one or more 5S rDNA hybridization sites and two 45S rDNA hybridization sites associated with the nucleolar periphery. The 45S rDNA hybridization patterns frequently exhibited gene puffs as de-condensed chromatin strings within the nucleoli. The other condensed rDNA sites (both 5S and 45S) were spatially distant from the nucleolus in nucleoplasmic regions containing heterochromatin. The distribution of rDNA between the nucleoplasm and the nucleoli is consistent with differential gene expression between homologous alleles and among homoeologous loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050258
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  • 9
    Electronic Resource
    Electronic Resource
    Rpg1, a soybean gene effective against races of bacterial blight, maps to a cluster of previously identified disease resistance genes (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1013-1021 
    Details
    ISSN: 1432-2242
    Keywords: Key words Nucleotide binding site ; Leucine-rich repeats ; RFLP mapping ; Recombinant-inbred lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Alleles, or tightly linked genes, at the soybean (Glycine max L. Merr.) Rpg1 locus confer resistance to races of Pseudomonas syringae pv. glycinea that express the avirulence genes avrB or avrRpm1. In this study we demonstrate that Rpg1 maps to a cluster of previously identified resistance genes, including those effective against fungal, viral and nematode pathogens. Rpg1 is in molecular linkage group (MLG) F, flanked by the markers K644 and B212. The RFLP markers R45, php2265 and php2385 cosegregated with Rpg1, as did the marker nbs61, which encodes a protein related to previously isolated resistance genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050833
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  • 10
    Electronic Resource
    Electronic Resource
    DNA methylation and AFLP marker distribution in the soybean genome (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 785-792 
    Details
    ISSN: 1432-2242
    Keywords: Key words Soybean ; Glycine max ; AFLP ; Methylation ; Genetic mapping ; Centromeres
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Amplified fragment length polymorphisms (AFLPs) have become important markers for genetic mapping because of their ability to reliably detect variation at a large number of loci. We report here the dissimilar distribution of two types of AFLP markers generated using restriction enzymes with varying sensitivities to cytosine methylation in the soybean genome. Initially, AFLP markers were placed on a scaffold map of 165 RFLP markers mapped in 42 recombinant inbred (F6:7) lines. These markers were selected from a map of over 500 RFLPs analyzed in 300 recombinant inbred (F6:7) lines generated by crossing BSR101×PI437.654. The randomness of AFLP marker map position was tested using a Poisson-model distribution. We found that AFLP markers generated using EcoRI/MseI deviated significantly from a random distribution, with 34% of the markers displaying dense clustering. In contrast to the EcoRI/MseI AFLP markers, PstI/MseI-generated AFLP markers did not cluster and were under represented in the EcoRI/MseI marker clusters. The restriction enzyme PstI is notably sensitive to cytosine methylation, and these results suggest that this sensitivity affected the distribution of the AFLP markers generated using this enzyme in the soybean genome. The common presence of one EcoRI/MseI AFLP cluster per linkage group and the infrequent presence of markers sensitive to methylation in these clusters are consistent with the low recombination frequency and the high level of cytosine methylation observed in the heterochromatic regions surrounding centromeres. Thus, the dense EcoRI/MseI AFLP marker clusters may be revealing structural features of the soybean genome, including the genetic locations of centromeres.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051297
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