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  • 1
    Electronic Resource
    Electronic Resource
    Response of different genotypes of Brassica carinata to microspore culture (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sixteen lines of Brassica carinata were evaluated for microspore culture and plant regeneration. The highest values of cell division and embryo yield resulted from buds between 2.5 and 3.5 mm long with 3 days of pretreatment at 32°C and plating densities of 100 000-150 000 microspores/ml. Ten out of 16 lines tested (63%) responded positively to microspore culture. In all breeding and F1 lines, both cell division and embryo yield varied over a wide range, but embryogenic response was higher in F1 lines than in the breeding lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.1999.118001079.x
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  • 2
    Electronic Resource
    Electronic Resource
    Bread-making quality in hexaploid tritordeum with substitutions involving chromosome 1D (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Hexaploid tritordeum, the amphiploid Hordeum chilense×Triticum turgidum, has potential for bread making. In order to estimate the potential of bread wheat chromosome 1D for improving the bread-making quality of tritordeum, and the processing properties and agronomic performance of euploid tritordeum, (1Hch)1D and (1A)1D substitution lines have been evaluated in field trials. No significant differences for agronomical traits were observed between the two substitution lines and the sister euploid tritordeum, except for the kernel weight of the (1Hch)1D tritordeum substitution, which was lower than that of euploid tritordeum. Gluten strength, estimated by alveograph deformation energy (W), and loaf volume were substantially higher in both substitution lines than in the euploid tritordeum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00806.x
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  • 3
    Electronic Resource
    Electronic Resource
    Doubled haploid lines of Brassica carinata with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Brassica carinata is a potential oilseed crop for the Mediterranean area. Chemical mutagenesis has been applied to microspores of B. carinata with the purpose of identifying lines with altered erucic acid content. From a population of nearly 400 doubled haploid plants recovered, nine lines have been identified that exhibit promising useful changes in erucic acid concentration in the seed oil. Three lines showed erucic acid contents below 25%, with a minimum of 17.1%, and in six lines the level of this fatty acid was greater than 52%. Changes in other fatty acids are also described and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00602.x
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  • 4
    Electronic Resource
    Electronic Resource
    Fluorescence and photochemical properties of phytochromes in wild-type wheat and a transgenic line overexpressing an oat phytochrome A (PHYA) gene: functional implications (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 24 (2001), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Etiolated seedlings of wild-type wheat and a transgenic line overexpressing an oat PHYA gene were investigated by the use of in situ low-temperature fluorescence spectroscopy. The red-absorbing phytochrome form, Pr, was characterized by (1) fluorescence emission spectrum; (2) total phytochrome content, and (3) by the extent of the Pr → lumi-R photoconversion at low temperature (γ1), and of the Pr → Pfr photoconversion at ambient temperature (γ2) as derived from emission data. All the characteristics were shown to be variable and to depend on (1) organ and tissue used; (2) seedling age; (3) transgenic wheat modification, and (4) continuous far-red irradiation of seedlings during their growth. These variations were interpreted in terms of the existence in wheat seedlings of the two phenomenological Pr types: (a), Pr′– major longer wavelength (687/673 nm, emission/absorption maxima) variable and light-labile with γ1 ≈ 0·5; and (b), Pr′′– minor, shorter wavelength (682/668 nm), relatively constant with its concentration not changing significantly with the increase of total phytochrome content in tissues and light-stable with γ1 ≤ 0·05–0·1. Overexpression of oat phyA increases primarily the content of Pr′ suggesting that it is comprised of phyA (phyA′) whereas Pr′′ is believed to consist of the minor phyA fraction (phyA′′) and phyB. The transgenic wheat line has been demonstrated to have a modified phenotype – the appearance of the far-red high irradiance reaction (FR-HIR) (Shlumukov et al. Plant, Cell and Environment 24, 703–712). The increased content of phyA′ in the transgenic line, whereas the total [phyA′′ + phyB] remains the same as in the wild type, indicates that the phyA′ pool is primarily responsible for the observed modification of the phenotype and suggests that even in wild-type plants the phyA′ component of the phyA pool may mediate the FR-HIR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2001.00780.x
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  • 5
    Electronic Resource
    Electronic Resource
    Establishment of far-red high irradiance responses in wheat through transgenic expression of an oat phytochrome A gene (2001)
    Oxford, UK : Blackwell Science, Ltd
    Plant, cell & environment 24 (2001), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A transgenic wheat line over-expressing an oat phytochrome A gene under the control of the constitutive maize ubiquitin promoter was generated using a biolistic particle delivery system from immature wheat embryos. The resulting line showed increased levels of total phytochrome A protein in both dark-grown and light-grown plants. When grown under continuous far-red light, seedlings of this line showed additional inhibition of the coleoptile extension in comparison with wild-type seedlings. Unlike the response of wild-type seedlings to continuous far-red, this additional inhibition was dependent on fluence rate and was not observed under half-hourly pulses of far-red delivering the same total fluence as the continuous irradiation treatment. These observations suggest that increase in phytochrome A levels in wheat leads to the establishment of a far-red high irradiation reaction in this monocotyledonous plant. Exposure to continuous red light caused a similar inhibition of coleoptile extension in both the wild types and the transgenic seedlings. When wild-type seedlings were grown under continuous far-red, their coleoptiles remained completely colourless and first leaves remained tightly rolled. In contrast, transgenic seedlings grown in the same conditions produced significant levels of anthocyanins in their coleoptiles and their first leaves became unrolled. Taken together, our data suggest that the increased levels of phytochrome A in wheat can change the type of response of some developmental processes to light signals, leading to the generation of a high irradiance reaction which is otherwise absent in the wild types under the conditions used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2001.00718.x
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  • 6
    Electronic Resource
    Electronic Resource
    Genomic influence on somatic embryogenesis in the Triticeae (1999)
    Springer
    Plant cell reports 18 (1999), S. 769-772 
    Details
    ISSN: 1432-203X
    Keywords: Key words Somatic embryogenesis ; Triticeae ; Amphiploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryogenesis is generally accepted to be under genetic control. The influence of genome interactions is not well understood given the difficulties of obtaining the appropriate vegetal material. Synthetic alloploids in which genomes from two species are fused together are suitable subject material to analyse this factor. In the Triticeae tribe amphiploids can be easily synthesised, which provides the opportunity to carry on this type of study. Crossing three autotetraploids, Hordeum chilense (HHHH), Triticum tauschii (DDDD) and Secale cereale (RRRR), we obtained three tetraploid amphiploids, ×Tritordeum (HHDD), ×Triticale (DDRR) and ×Hordecale (HHRR). Somatic embryogenesis from immature inflorescence and flag leaves were scored on parents and amphiploids. Immature inflorescences had a higher embryogenic response than the flag leaves. The amphiploids showed higher regeneration ability than their parents. The best genomic combination was the tetraploid triticale DDRR for every inflorescence and flag leaf size tested, followed by HHDD and HHRR. Heterosis was found to be the main genetic factor affecting the in vitro culture response although there are clear differences among the three amphiploids tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050658
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  • 7
    Electronic Resource
    Electronic Resource
    The influence of auxins on transformation of wheat and tritordeum and analysis of transgene integration patterns in transformants (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 684-695 
    Details
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Auxins ; Bombardment ; Transformation efficiency ; Transgene integration pattern
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Although it is possible to transform wheat, broad application of the technology is limited because of the low overall efficiency and the lack of reliability of the technique. In addition there is little published data on transgene integration patterns and inheritance in wheat. We have generated a population of transgenic wheat and tritordeum lines under different auxin regimes and show that, under the conditions described, the presence of picloram results in higher transformation efficiencies than the presence of 2,4-D. Molecular analysis shows low-copy numbers and simple integration patterns to be prevalent in the transgenic lines. Mendelian inheritance of transgenes in T 1 progeny was observed for the majority of lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050944
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  • 8
    Electronic Resource
    Electronic Resource
    Altered functional properties of tritordeum by transformation with HMW glutenin subunit genes (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 851-858 
    Details
    ISSN: 1432-2242
    Keywords: Key words Tritordeum ; Transformation ; HMW glutenin subunits ; Seed protein engineering ; Dough functionality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The high-molecular-weight (HMW) subunits of wheat glutenin are the major determinants of the gluten visco-elasticity that allows wheat doughs to be used to make bread, pasta and other food products. In order to increase the proportions of the HMW subunits, and hence improve breadmaking performance, particle bombardment was used to transform tritordeum, a fertile amphiploid between wild barley and pasta wheat, with genes encoding two HMW glutenin subunits (1Ax1 and 1Dx5). Of the 13 independent transgenic lines recovered (a transformation frequency of 1.4%) six express the novel HMW subunits at levels similar to, or higher than, those of the endogenous subunits encoded on chromosome 1B. Small-scale mixograph analysis of T2 seeds from a line expressing the transgene for 1Dx5 indicated that the addition of novel HMW subunits can result in significant improvements in dough strength and stability, thus demonstrating that transformation can be used to modify the functional properties of tritordeum for improved breadmaking.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051305
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  • 9
    Electronic Resource
    Electronic Resource
    Organic nitrogen content and nitrate and nitrite reductase activities in tritordeum and wheat grown under nitrate or ammonium (1991)
    Springer
    Plant and soil 135 (1991), S. 251-256 
    Details
    ISSN: 1573-5036
    Keywords: nitrate reductase ; nitrite reductase ; organic nitrogen ; tritordeum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tritordeum is a fertile amphiploid derived from durum wheat (Triticum turgidum L. conv. durum) × a wild barley (Hordeum chilense Roem. et Schultz.). The organic nitrogen content of tritordeum grain (34 mg g-1 DW) was significantly higher than that of its wheat parent (25 mg g-1 DW). Leaf and root nitrogen content became higher in tritordeum than in wheat after four weeks of growth, independently of the nitrogen source (either NO3 - or NH4 +). Under NO3 - nutrition, tritordeum generally exhibited higher levels of nitrate reductase (NR) activity than wheat. Nitrite reductase (NiR) levels were however lower in tritordeum than in its wheat parent. In NH4 +-grown plants, both NR and NiR activities progressively decreased in the two species, becoming imperceptible after 3 to 5 weeks of growth. Results indicate that, in addition to a higher rate of NO3 - reduction, other physiological factors must be responsible for the greater accumulation of organic nitrogen in tritordeum grain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00010913
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  • 10
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    Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference (2010)
    National Academy of Sciences
    Details
    Publication Date: 2010-09-09
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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