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  • 1
    ISSN: 1420-9136
    Keywords: Radon ; Anomaly ; Earthquake ; New Madrid Seismic zone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Anomalous soil-radon activity, including several spike-like surges over periods of 5, 2, and 2 1/2 months, and a year-long declining trend, preceded the most significant earthquakes of the central mid-continental region of North America during 1981 and early 1984. The 5-month period of erratic weekly radon activity, February–June 1981, was followed by a tremor of magnitudeM=4.0, 40 km from the monitoring site in the New Madrid seismic zone. An unusual earthquake swarm in central Arkansas, 160 km from the New Madrid seismic zone and 230 km from the monitoring site in the seismic zone, began in January 1982, shortly after a year-long declining trend in anomalous radon emanation. Earthquakes of magnitudes 4.5, 4.1, and 4.0 occurred at the swarm's outset in early 1982, fitting a pattern anticipated for the New Madrid seismic zone on the basis of the radon activity. Two periods of coincident peak radon emanation have since been observed in the Arkansas and New Madrid seismic regions, as have synchronous seismic pulses for the two separate areas. Two more recent periods of highly erratic soil-radon emanation, March–May 1983 and November–January 1984, were followed by a 4.3 earthquake in southwestern Illinois on 15 may 1983, and 3.5–3.6 tremors and swarm activity in the New Madrid seismic zone in late January and mid-February 1984. Prior to the 4.3 event, radon peaked at three widely separated monitoring sites 1–3 weeks before the tremor at distances of 120, 225, and 320 km from the epicentral region, the station at 225 km, in the New Madrid seismic zone, recording the longest period of anomalous radon activity. As for the recent 3.5–3.6 tremors of 1984, seismic activity of this magnitude had been anticipated for January or February on the basis of the amplitude of the November radon anomaly. These observations provide further evidence of (a) the existence of soil-radon anomalies precursory to the large earthquakes in this intraplate region, (b) the utility of such anomalies in anticipating events of small to moderate magnitudes for the region, and (c) the occurrence of regional-scale strain events prior to some of the larger mid-continental earthquakes. A very recent radon anomaly, the strongest yet to be detected in the seven years of monitoring in the mid-continental region, occurred in the New Madrid seismic zone from mid-February through mid-June 1984. A 4.0 earthquake occurred one month after a peak in the radon activity. The amplitude and duration of the anomaly suggest that a significant change in the state of stress or strain may have occurred in the mid-continental region during 1984.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 221 (1969), S. 360-361 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We administered 2 mCi of 4-14C-ethynyl oestradiol (specific activity 40 mCi/mg prepared from 4-14C-oestrone2) intravenously to two women and orally in a gelatine capsule to a further five women. Urine samples were collected over 5 days and blood samples were taken during the 24 h after ...
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  • 3
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Anthropology 18 (1989), S. 157-175 
    ISSN: 0084-6570
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Ethnic Sciences , Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 74 (1987), S. 140-146 
    ISSN: 1432-2242
    Keywords: Wheat ; Protoplasts ; Cell suspensions ; Aneuploidy ; Structural chromosome variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cytology of bread wheat (Triticum aestivum) suspension lines, recycled lines (selected for high division frequency) and their dividing protoplasts, have been examined. Extensive numerical and structural chromosome variation was present in all the lines. The most frequently observed chromosome numbers were around 2n=32, indicating that considerable chromosome loss from the normal wheat complement (2n=6x=42) had occurred during selection of the lines. Chromosome aberrations also indicated loss of chromosome arms and chromosome segments. The implications of this variation for studies on transformation and for the potential regeneration of whole plants from protoplasts of bread wheat are discussed.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 76 (1988), S. 880-886 
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Solanum brevidens ; Somatic hybrids ; Planned release ; Field trial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Following both chemical and electrical fusion of protoplasts of a dihaploid line of potato (Solanum tuberosum), (PDH40), with those of the wild species, Solanum brevidens, 11 and 40 somatic hybrid plants, respectively were obtained. Fifteen of these somatic hybrid genotypes and the two parents were studied further in a small field trial to assess field performance and phenotypic variability. In the UK, somatic hybrid plants are classified as genetically engineered organisms, and the UK Advisory Committee on Genetic Manipulation have imposed various restrictions on field experiments. Examination of the somatic hybrids in the field showed extensive phenotypic variability, and no two genotypes were identical. Some of the variation reflected changes in chromosome numbers, but other factors were also involved. Half the somatic hybrid genotypes produced tubers in the field, although the tubers were smaller and differed morphologically from those of PDH40. The results of the study suggest that the extent of somaclonal variation manifested in somatic hybrids is greater than that found in protoplast-derived plants of potato. The implications of this observation and the current regulations concerning field experiments of somatic hybrid plants in the UK are discussed.
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  • 6
    ISSN: 1432-2242
    Keywords: Potato ; Solanum brevidens ; Somatic hybrids ; Characterization (molecular, cytological, morphological)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fifty-eight somatic hybrid plants, produced both by chemical (11) and electrical fusion (47) of protoplasts of dihaploid Solanum tuberosum and S. brevidens, have been analysed by molecular, cytological and morphological methods. The potentially useful euploid plants constituted 34% of the total, of which 20% were tetraploid and 14% hexaploid; the remainder were aneuploid at the tetraploid, hexaploid and octoploid levels. Analysis of chloroplast DNA showed that 55% of hybrids contained chloroplasts from S. brevidens and 45% from S. tuberosum. Hexaploids, the products of three protoplasts fusing together, were analyzed with specific DNA probes, and this revealed that nuclear genome dosages could be either 2∶1 S. tuberosum∶S. brevidens, or vice-versa. Chloroplast types of hexaploids were not influenced by nuclear genome dosage, and all six possible combinations of genome dosage and chloroplast types were found amongst tetraploids and hexaploids. To examine the morphology of the hybrid population and its possible relation to the chromosome number and chloroplast DNA type, 18 morphological characteristics were measured on greenhouse-grown plants and analyzed by principal component and canonical variate analyses. Both analyses showed that nuclear ploidy has the most prominent influence on the overall morphology of the hybrids. Differential parental genome expression in the morphology of the hybrids is discussed. These results provide useful data on the range of genetic combinations that can be expected to occur amongst somatic hybrid plants.
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  • 7
    ISSN: 1432-2242
    Keywords: Key words Tritordeum ; Transformation ; HMW glutenin subunits ; Seed protein engineering ; Dough functionality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The high-molecular-weight (HMW) subunits of wheat glutenin are the major determinants of the gluten visco-elasticity that allows wheat doughs to be used to make bread, pasta and other food products. In order to increase the proportions of the HMW subunits, and hence improve breadmaking performance, particle bombardment was used to transform tritordeum, a fertile amphiploid between wild barley and pasta wheat, with genes encoding two HMW glutenin subunits (1Ax1 and 1Dx5). Of the 13 independent transgenic lines recovered (a transformation frequency of 1.4%) six express the novel HMW subunits at levels similar to, or higher than, those of the endogenous subunits encoded on chromosome 1B. Small-scale mixograph analysis of T2 seeds from a line expressing the transgene for 1Dx5 indicated that the addition of novel HMW subunits can result in significant improvements in dough strength and stability, thus demonstrating that transformation can be used to modify the functional properties of tritordeum for improved breadmaking.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1572-9788
    Keywords: Triticum turgidum L. var. durum ; pasta wheat ; transformation ; seed protein modification ; flour quality improvement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Particle bombardment has been used to transform three cultivars (L35, Ofanto, Svevo) and one breeding line (Latino × Lira) of durum wheat (Triticum turgidum L. var. durum). These varieties were co-transformed with plasmids containing selectable and scorable marker genes (bar and uidA) and plasmids containing one of two high-molecular-weight (HMW) glutenin subunit genes (encoding subunits 1Ax1 or 1Dx5). Ten independent transgenic lines were recovered from 1683 bombarded scutella (transformation efficiency thus 0.6%). Five lines expressed either subunit 1Dx5 or 1Ax1 at levels similar to those of endogenous subunits encoded on chromosome 1B. To identify the effects of the transgenes on the functional properties of grain, three lines showing segregation for transgene expression were used to isolate sibling T2 plants which were null or positive for the transgene product. Analysis of these plants using a small-scale mixograph showed that expression of the additional subunits resulted in increased dough strength and stability, demonstrating that transformation can be used to modify the quality of durum wheat for bread and pasta making.
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  • 10
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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