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  • Cells, Cultured  (92)
  • American Association for the Advancement of Science (AAAS)  (92)
  • American Chemical Society
  • American Meteorological Society
  • 1980-1984  (92)
  • 1925-1929
  • 1983  (47)
  • 1982  (45)
Collection
Keywords
Publisher
  • American Association for the Advancement of Science (AAAS)  (92)
  • American Chemical Society
  • American Meteorological Society
Years
  • 1980-1984  (92)
  • 1925-1929
Year
  • 1
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    Radiosensitization of hypoxic tumor cells by depletion of intracellular glutathione (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-08-06
    Description: Depletion of glutathione in Chinese hamster ovary cells in vitro by diethyl maleate resulted in enhancement of the effect of x-rays on cell survival under hypoxic conditions but not under oxygenated conditions. Hypoxic EMT6 tumor cells were similarly sensitized in vivo. The action of diethyl maleate is synergistic with the effect of the electron-affinic radiosensitizer misonidazole, suggesting that the effectiveness of misonidazole in cancer radiotherapy may be improved by combining it with drugs that deplete intracellular glutathione.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bump, E A -- Yu, N Y -- Brown, J M -- CA-15201/CA/NCI NIH HHS/ -- CM-87207/CM/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Aug 6;217(4559):544-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7089580" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Anoxia ; Cell Survival/drug effects/*radiation effects ; Cells, Cultured ; Cricetinae ; Cricetulus ; Drug Synergism ; Glutathione/*metabolism ; Maleates/administration & dosage ; Mice ; Mice, Inbred BALB C ; Misonidazole/administration & dosage ; Neoplasms, Experimental/metabolism ; *Oxygen Consumption
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
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    In vitro Evidence for two distinct hippocampal growth factors: basis of neuronal plasticity? (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-07-02
    Description: The rat hippocampal formation was tested for the presence of factors that would accelerate neurite extension from chick parasympathetic (ciliary ganglion) or sympathetic (lumbar chain) neurons in vitro. Two growth factors were identified in extracts of this brain region. One accelerated neurite extension from sympathetic neurons and was blocked by antiserum to nerve growth factor. The other accelerated neurite extension from parasympathetic neurons but was not affected by the antiserum. These results suggest that specific growth factors account for the specificity of neuronal sprouting.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Crutcher, K A -- Collins, F -- NS 17131/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1982 Jul 2;217(4554):67-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7089542" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Axons/physiology ; Cells, Cultured ; Chick Embryo ; Ganglia, Parasympathetic/physiology ; Ganglia, Sympathetic/physiology ; Growth Substances/*physiology ; Hippocampus/*physiology ; Neurons/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    Receptors for maleylated proteins regulate secretion of neutral proteases by murine macrophages (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-11-05
    Description: Receptors for maleylated or acetylated proteins as well as for alpha-2-macroglobulin-protease complexes on macrophages serve as scavengers by mediating the uptake of macromolecules from the extracellular compartment. Described in this report is a novel function of these receptors on macrophages: regulation of neutral protease secretion. The binding of maleylated bovine serum albumin to macrophages triggered secretion of three neutral proteases: neutral caseinases, plasminogen activator, and cytolytic proteinase. Release of acid phosphatase, however, was not induced. An important biological consequence of protease secretion by macrophages, tumor-cytolysis, was also triggered by engagement of the receptor for maleylated bovine serum albumin. By contrast, the binding of alpha-2-macroglobulin-protease complexes to the macrophages suppressed secretion of all three proteases. Thus two receptors heretofore believed to serve principally as scavengers also regulate secretory functions of macrophages.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Johnson, W J -- Pizzo, S V -- Imber, M J -- Adams, D O -- New York, N.Y. -- Science. 1982 Nov 5;218(4572):574-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6289443" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Glycoproteins/*metabolism ; Macrophages/*enzymology ; *Metalloendopeptidases ; Mice ; Peptide Hydrolases/*secretion ; Plasminogen Activators/secretion ; Receptors, Cell Surface/*physiology
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Eukaryotic transcriptional regulation and chromatin-associated protein phosphorylation by cyclic AMP (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-12-24
    Description: Cyclic adenosine monophosphate (AMP) analogs or agents that increase intracellular cyclic AMP rapidly stimulate transcription of the prolactin gene in a line of cultured rat pituitary cells. This effect is correlated with the phosphorylation of a chromatin-associated basic protein designated BPR. These data are consistent with the postulate that increased intracellular cyclic AMP concentrations induce rapid transcriptional effects on specific genes in eukaryotes, mediated by direct or indirect phosphorylation of a specific chromatin-associated protein or proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Murdoch, G H -- Rosenfeld, M G -- New York, N.Y. -- Science. 1982 Dec 24;218(4579):1315-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6293056" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Chromatin/*metabolism ; Cyclic AMP/analogs & derivatives/*metabolism ; Nucleoproteins/metabolism ; Phosphorylation ; Pituitary Gland/metabolism ; Prolactin/genetics ; Rats ; *Transcription, Genetic
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    Brain injury causes a time-dependent increase in neuronotrophic activity at the lesion site (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-08-27
    Description: A cavity was made in the brain (entorhinal cortex) of developing or adult rats, and a small piece of Gelfoam was emplaced to collect fluid secreted into the wound. The neuronotrophic activity of the fluid was assayed with sympathetic and parasympathetic neurons in culture. The results show that wounds in the brain of developing or adult rats stimulate the accumulation of neuronotrophic factors and that the activity of these factors increases over the first few days after infliction of the damage.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nieto-Sampedro, M -- Lewis, E R -- Cotman, C W -- Manthorpe, M -- Skaper, S D -- Barbin, G -- Longo, F M -- Varon, S -- AG-00538/AG/NIA NIH HHS/ -- MH-19691/MH/NIMH NIH HHS/ -- NS-16349/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1982 Aug 27;217(4562):860-1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7100931" target="_blank"〉PubMed〈/a〉
    Keywords: Adrenergic Fibers/physiology ; Animals ; Brain/*physiology ; Brain Injuries/*physiopathology ; Cell Survival/drug effects ; Cells, Cultured ; Cholinergic Fibers/physiology ; Kinetics ; Nerve Growth Factors/*metabolism/pharmacology ; *Nerve Regeneration ; Rats ; Rats, Inbred Strains ; Wound Healing
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    Proliferative capacity of murine hematopoietic stem cells in vitro (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-03-26
    Description: Large numbers of granulocytes can be collected repeatedly from the supernatant medium of long-term cultures of mouse bone marrow cells. A constant relationship was found between the number of adherent hematopoietic stem cells and the lifetime cell production per culture. The data indicate that there is a limit to the proliferative capacity of normal and of irradiated stem cells. A similar limitation was found in the production of marked granulocytes from clonal cultures of "beige" C57 (bg/bgJ) stem cells placed in limiting dilutions into stromal culture layers.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Reincke, U -- Hannon, E C -- Rosenblatt, M -- Hellman, S -- CA 10941/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Mar 26;215(4540):1619-22.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7071580" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Bone Marrow Cells ; Cell Division/radiation effects ; Cells, Cultured ; Granulocytes/physiology ; *Hematopoiesis/radiation effects ; Hematopoietic Stem Cells/*cytology ; Mice ; Spleen/cytology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    Hormonal regulation of gonadotropin receptors and steroidogenesis in cultured fetal rat tests (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-10-22
    Description: Gonadotropic activation of the adult rat testis in vitro and in vivo is followed by down-regulation of luteinizing hormone receptors and decreased androgen responses to subsequent hormonal stimulation. In contrast, treatment of cultured fetal testes with gonadotropins and dibutyryl adenosine 3',5'-monophosphate enhanced steroidogenic responsiveness and did not cause the luteinizing hormone-receptor loss and desensitization that is characteristic of the adult gonad. The analysis of gonadotropin receptors and action in cultured fetal testis cells facilitates developmental studies of gonadal function, and has revealed significant differences in the responses of fetal and adult Leydig cells to gonadotropic regulation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Warren, D W -- Dufau, M L -- Catt, K J -- 1F33-HD06192/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1982 Oct 22;218(4570):375-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6289438" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Bucladesine/pharmacology ; Cell Differentiation/drug effects ; Cells, Cultured ; Chorionic Gonadotropin/pharmacology ; Hydroxyprogesterones/biosynthesis ; Leydig Cells/*drug effects ; Luteinizing Hormone/pharmacology ; Male ; Progesterone/biosynthesis ; Rats ; Receptors, Cell Surface/*drug effects/metabolism ; Receptors, LH ; Testis/*embryology/metabolism ; Testosterone/biosynthesis
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    Homeostasis of the antibody response: immunoregulation by NK cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-11-11
    Description: When injected into mice, the synthetic double-stranded polynucleotide poly(inosinic) X poly(cytidylic) acid induces high natural killer (NK) cell activity within 4 to 12 hours. Induction of NK activity in mice immunized 2 or 3 days previously, or the addition of NK cells to cultures immunized in vitro 2 or 3 days previously, promotes early termination of the ongoing primary immunoglobulin M antibody response. A target for NK cells is a population of accessory cells that has interacted with antigen and is necessary for sustaining the antibody response. The inference is strong that NK cells induced normally by immunization also terminate the usual antibody response in vivo by elimination of antigen-exposed accessory cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Abruzzo, L V -- Rowley, D A -- 5-T32-CA-09267/CA/NCI NIH HHS/ -- R01-10242/PHS HHS/ -- New York, N.Y. -- Science. 1983 Nov 11;222(4624):581-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6685343" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Antibody Formation ; Antibody-Producing Cells/immunology ; Cells, Cultured ; Homeostasis ; Killer Cells, Natural/*immunology/radiation effects ; Lymphocyte Cooperation ; Lymphocytes/*immunology ; Mice ; Poly I-C/immunology ; Spleen/immunology
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
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    Primary murine bone marrow cultures support continuous growth of infectious human trypanosomes (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-04-22
    Description: The human parasite Trypanosoma brucei gambiense grew continuously at 37 degrees C in primary cultures of murine bone marrow. Cultured parasites remained virulent for mice. Rapid parasite growth coincided with the appearance of adherent adipocyte-epitheloid cell aggregates that also promoted hematopoiesis. This culture system should permit studies of host cell control of trypanosome proliferation, pathogenic effects of trypanosomes on blood cell development, and the relative trypanocidal and marrow suppressive activities of drugs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Balber, A E -- CA 14049/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1983 Apr 22;220(4595):421-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6836284" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Bone Marrow ; Cells, Cultured ; Culture Media ; Humans ; Mice ; Mice, Inbred BALB C ; Trypanosoma brucei brucei/growth & development ; Trypanosoma brucei gambiense/*growth & development ; Trypanosomiasis, African/parasitology
    Print ISSN: 0036-8075
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  • 10
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    Wound tissue can utilize a polymeric template to synthesize a functional extension of skin (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-01-08
    Description: Prompt and long-term closure of full-thickness skin wounds is guinea pigs and humans is achieved by applying a bilayer polymeric membrane. The membrane comprises a top layer of a silicone elastomer and a bottom layer of a porous cross-linked network of collagen and glycosaminoglycan. The bottom layer can be seeded with a small number of autologous basal cells before grafting. No immunosuppression is used and infection, exudation, and rejection are absent. Host tissue utilizes the sterile membrane as a culture medium to synthesize neoepidermal and neodermal tissue. A functional extension of skin over the entire wound area is formed in about 4 weeks.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yannas, I V -- Burke, J F -- Orgill, D P -- Skrabut, E M -- GM 21700/GM/NIGMS NIH HHS/ -- GM 23946/GM/NIGMS NIH HHS/ -- HL 14322/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1982 Jan 8;215(4529):174-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7031899" target="_blank"〉PubMed〈/a〉
    Keywords: Adolescent ; Adult ; Animals ; Burns/*therapy ; Cells, Cultured ; Child ; Child, Preschool ; Collagen/therapeutic use ; Female ; Glycosaminoglycans/therapeutic use ; Guinea Pigs ; Humans ; Male ; Middle Aged ; Silicone Elastomers/therapeutic use ; *Skin Transplantation ; *Wound Healing
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 11
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    Collagen formation by the hepatocyte in primary monolayer culture and in vivo (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-03-18
    Description: Immunohistochemical techniques were used to confirm biochemical evidence that parenchymal cells isolated from adult rat liver and maintained in nonreplicating monolayer culture for 2 days synthesized type IV basement membrane collagen. On continued incubation in serum-free medium, the hepatocytes also synthesized the interstitial collagens, types I and III. Consistent with these results in culture, type IV collagen was localized to the hepatocytes in slices of pathologic rat liver. Hence collagen formation is a previously unrecognized function of the hepatocyte that may be important in the pathogenesis of liver fibrosis or cirrhosis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Diegelmann, R F -- Guzelian, P S -- Gay, R -- Gay, S -- AM18976/AM/NIADDK NIH HHS/ -- DE02570/DE/NIDCR NIH HHS/ -- HL11310/HL/NHLBI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1983 Mar 18;219(4590):1343-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6828863" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Basement Membrane/metabolism ; Cells, Cultured ; Collagen/*biosynthesis/immunology ; Liver/cytology/*metabolism ; Molecular Weight ; Rats
    Print ISSN: 0036-8075
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  • 12
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    Transformation of Bloom's syndrome fibroblasts by DNA transfection (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-12-09
    Description: Nonmalignant diploid human fibroblast cells (GM3498B) derived from a skin biopsy of a patient with Bloom's syndrome have been transformed by transfection with DNA from a tumorigenic mouse cell line (Ha-8) carrying a single copy of the Harvey murine sarcoma virus (Ha-MuSV) genome. The transformed cell lines have an extended life-span, form colonies in agarose, and proliferate in nude mice--characteristics of neoplastic transformation. Like the parental cells, they also exhibit a high spontaneous level of sister chromatid exchanges. Finally, the transformed cells contain most, if not all, of the Ha-MuSV genome as well as the human rasH sequence. These experiments show that these diploid nonmalignant human cells can be used as recipients in transfection experiments for studying the genetic control of neoplastic transformation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Doniger, J -- Di Paolo, J A -- Popescu, N C -- New York, N.Y. -- Science. 1983 Dec 9;222(4628):1144-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6648529" target="_blank"〉PubMed〈/a〉
    Keywords: Bloom Syndrome/*genetics ; Cell Adhesion ; *Cell Transformation, Neoplastic ; Cells, Cultured ; DNA, Neoplasm/*genetics ; Humans ; Oncogenes ; Transfection
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  • 13
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    Formaldehyde damage to DNA and inhibition of DNA repair in human bronchial cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-04-08
    Description: Cultured bronchial epithelial and fibroblastic cells from humans were used to study DNA damage and toxicity caused by formaldehyde. Formaldehyde caused the formation of cross-links between DNA and proteins, caused single-strand breaks in DNA, and inhibited the resealing of single-strand breaks produced by ionizing radiation. Formaldehyde also inhibited the unscheduled DNA synthesis that occurs after exposure of cells to ultraviolet irradiation or to benzo[a]pyrene diolexpoxide but at doses substantially higher than those required to inhibit the resealing of x-ray-induced single-strand breaks. Therefore, formaldehyde could exert its mutagenic and carcinogenic effects by both damaging DNA and inhibiting DNA repair.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Grafstrom, R C -- Fornace, A J Jr -- Autrup, H -- Lechner, J F -- Harris, C C -- New York, N.Y. -- Science. 1983 Apr 8;220(4593):216-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6828890" target="_blank"〉PubMed〈/a〉
    Keywords: Bronchi/*cytology/drug effects ; Cells, Cultured ; *DNA/biosynthesis ; DNA Repair/*drug effects ; Epithelium/drug effects ; Fibroblasts/drug effects ; Formaldehyde/*pharmacology ; Humans
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  • 14
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    Neuron-glia adhesion is inhibited by antibodies to neural determinants (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-10-07
    Description: Suspensions of embryonic chick neuronal cells adhered to monolayers of glial cells, but few neurons bound to control monolayers of fibroblastic cells from meninges or skin. Neuronal cell-glial cell adhesion was inhibited by prior incubation of the neurons with Fab' fragments of antibodies to neuronal membranes. In contrast, antibodies to the neural cell adhesion molecule (N-CAM) did not inhibit the binding. These results suggest that a specific adhesive mechanism between neurons and glial cells exists and that it is mediated by CAM's that differ from those so far identified.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Grumet, M -- Rutishauser, U -- Edelman, G M -- AI-11378/AI/NIAID NIH HHS/ -- HD-09635/HD/NICHD NIH HHS/ -- HD-16550/HD/NICHD NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1983 Oct 7;222(4619):60-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6194561" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibodies, Monoclonal ; Antigen-Antibody Complex ; *Cell Adhesion ; Cell Membrane/immunology ; Cells, Cultured ; Chick Embryo ; Epitopes ; Immunoglobulin Fab Fragments ; Neuroglia/*physiology ; Neurons/immunology/*physiology
    Print ISSN: 0036-8075
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  • 15
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    Tumor-derived growth factor increases bone resorption in a tumor associated with humoral hypercalcemia of malignancy (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-09-23
    Description: Evidence is presented that a tumor-derived transforming growth factor is responsible for stimulating bone resorption and causing hypercalcemia in an animal tumor model of the hypercalcemia of malignancy. Both conditioned medium harvested from cultured tumor cells and tumor extracts of the transplantable rat Leydig cell tumor associated with hypercalcemia contained a macromolecular bone resorbing factor with the chemical characteristics of a tumor-derived transforming growth factor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ibbotson, K J -- D'Souza, S M -- Ng, K W -- Osborne, C K -- Niall, M -- Martin, T J -- Mundy, G R -- AM-28149/AM/NIADDK NIH HHS/ -- CA-29537/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1983 Sep 23;221(4617):1292-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6577602" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Bone Resorption ; Calcium ; Cells, Cultured ; Culture Media ; Growth Substances/*physiology ; Hypercalcemia/*etiology ; Leydig Cell Tumor/complications/*physiopathology ; Male ; Neoplasm Proteins/*physiology ; Neoplasms, Experimental/complications/physiopathology ; Peptides/*physiology ; Rats ; Transforming Growth Factors
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  • 16
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    Fragile sites in chromosomes: possible model for the study of spontaneous chromosome breakage (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-04-01
    Description: The tissue culture condition that is required for the type of chromosome breakage seen at most fragile sites, namely, the absence of folic acid and thymidine in the medium, greatly enhanced micronucleus formation in proliferating lymphocyte cultures from normal individuals. This suggests that chromosome breakage at fragile sites and the apparently spontaneous damage that gives rise to micronuclei are controlled by the same mechanism.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jacky, P B -- Beek, B -- Sutherland, G R -- New York, N.Y. -- Science. 1983 Apr 1;220(4592):69-70.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6828880" target="_blank"〉PubMed〈/a〉
    Keywords: Adolescent ; Adult ; Cell Nucleus/drug effects/ultrastructure ; Cells, Cultured ; Child ; *Chromosome Aberrations ; Chromosome Fragile Sites ; *Chromosome Fragility ; Culture Media ; Dose-Response Relationship, Drug ; Female ; Folic Acid/pharmacology ; Humans ; Lymphocytes/ultrastructure ; Male ; Middle Aged ; Thymidine/pharmacology
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  • 17
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    Oxygen tension regulates the expression of angiogenesis factor by macrophages (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-09-23
    Description: When cultured in a hypoxic environment similar to that found in the center of a wound, macrophages secreted active angiogenesis factor into the medium. Under conditions similar to those of well-oxygenated tissue, macrophages did not secrete active angiogenesis factor. Macrophages that secreted the factor at hypoxic conditions stopped secreting it when returned to room air. Thus the control of angiogenesis in wound healing may be the result of macrophages responding to tissue oxygen tension without the necessity of interacting with other cell types or biochemical signals.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Knighton, D R -- Hunt, T K -- Scheuenstuhl, H -- Halliday, B J -- Werb, Z -- Banda, M J -- GM27345/GM/NIGMS NIH HHS/ -- HL26323/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1983 Sep 23;221(4617):1283-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6612342" target="_blank"〉PubMed〈/a〉
    Keywords: Angiogenesis Inducing Agents/*biosynthesis ; Animals ; Anoxia/physiopathology ; Cells, Cultured ; Cornea ; Growth Substances/*biosynthesis ; Macrophages/*physiology ; Models, Biological ; Oxygen/*physiology ; Rabbits ; *Wound Healing
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  • 18
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    Drug-induced alterations of cytokeratin organization in cultured epithelial cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-02-04
    Description: The distribution of keratin intermediate filaments, previously considered static in organization and imperturbable by conventional drugs used to alter the structure and organization of the cytoskeleton, can be altered significantly by treatment with colchicine and cytochalasin D. The loss of microfilaments and microtubules converts the keratin cytoskeleton from a branching, even distribution to a series of starlike structures whose filaments are maintained by multiple membrane attachment sites. These findings provide a means for manipulating cytokeratin organization to investigate the role of keratins in cytoskeletal structure and function.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Knapp, L W -- O'Guin, W M -- Sawyer, R H -- New York, N.Y. -- Science. 1983 Feb 4;219(4584):501-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6186022" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Colchicine/*pharmacology ; Cytochalasin D ; Cytochalasins/*pharmacology ; Cytoskeleton/*drug effects ; Epithelium ; *Keratins ; Mice ; Microtubules/drug effects
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  • 19
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    Human endothelial cells: use of heparin in cloning and long-term serial cultivation (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-11-11
    Description: Endothelial cells from human blood vessels were cultured in vitro, with doubling times of 17 to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were established for the first time and had similar proliferative capacities. This vigorous cell growth was achieved by addition of heparin to culture medium containing reduced concentrations of endothelial cell growth factor. The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thornton, S C -- Mueller, S N -- Levine, E M -- AG-00839/AG/NIA NIH HHS/ -- T32-CA-09171/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1983 Nov 11;222(4624):623-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6635659" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Division/drug effects ; Cells, Cultured ; Clone Cells/enzymology ; Endothelium/*cytology ; Growth Substances/pharmacology ; Heparin/*pharmacology ; Humans ; Time Factors
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  • 20
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    Interaction with membrane remnants of target myotubes maintains transmitter sensitivity of cultured neurons (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-05-27
    Description: Parasympathetic neurons, when cultured alone, lose sensitivity to acetylcholine, but if striated muscle is included in the culture, neuronal chemosensitivity is maintained. The membrane remnants of myotubes ruptured by osmotic shock also supported the responsiveness of the cultured neurons to transmitter, whereas muscle-conditioned medium or membrane remnants of nonmuscle embryonic skin cells did not support this responsiveness. The regulation of chemosensitivity by contact of neurons with the target cell membrane may be important in the formation and maintenance of neuronal circuitry.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tuttle, J B -- NS-10338/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1983 May 27;220(4600):977-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6133352" target="_blank"〉PubMed〈/a〉
    Keywords: Acetylcholine/physiology ; Animals ; Cell Membrane/physiology ; Cells, Cultured ; Chick Embryo ; Fibroblasts/physiology ; Muscles/*physiology ; Nervous System/growth & development ; Neurons/*physiology ; Neurotransmitter Agents/*physiology ; Synapses/physiology
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  • 21
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    High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-10-28
    Description: A protoplast fusion method was developed to stably transfect human cells with pSV2-derived plasmids at frequencies greater than 10(-3). This procedure made it possible to test the biological effect of a hepatitis B virus (HBV) gene independent of the viral structures required for infection. A pSV2gpt+ plasmid constructed to carry a subgenomic fragment of HBV that contained the core antigen gene (HBc gene) was transfected into human cells. A human epithelial cell line was stably transfected with the HBc+ gene by selecting recipient cells for expression of guanine phosphoribosyl transferase expression. With this gpt+/HBc+ cell line it was shown that growth in serum-free medium or treatment with 5'-azacytidine stimulates the production of the HBV core antigen. A hepatocellular carcinoma carrying the entire HBV genome was stimulated to produce the HBc gene product in response to the same factors that stimulated HBcAg production in the gpt+/HBc+ cell line constructed by transfection. The temporal relation between the cytopathologic response and HBc gene expression was similar for both cell types, indicating a primary role for HBc gene expression in the cytopathology of HBV-infected human liver.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yoakum, G H -- Korba, B E -- Lechner, J F -- Tokiwa, T -- Gazdar, A F -- Seeley, T -- Siegel, M -- Leeman, L -- Autrup, H -- Harris, C C -- New York, N.Y. -- Science. 1983 Oct 28;222(4622):385-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6194563" target="_blank"〉PubMed〈/a〉
    Keywords: Azacitidine/pharmacology ; Cell Fusion ; *Cell Transformation, Viral ; Cells, Cultured ; Cytopathogenic Effect, Viral ; Gene Expression Regulation/drug effects ; Genes, Viral ; Hepatitis B Core Antigens/*genetics ; Humans ; Transfection
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  • 22
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    Insulin receptor: evidence that it is a protein kinase (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-01-21
    Description: Highly purified preparations of insulin receptor catalyzed the phosphorylation of the 95,000-dalton subunit of the insulin receptor. This subunit of the insulin receptor was also labeled with [alpha-32P]8-azidoadenosine 5'-triphosphate, a photoaffinity label for adenosine triphosphate binding sites. The identity of the 95,000-dalton band was confirmed in both cases by precipitation with a monoclonal antibody to the insulin receptor. These results suggest that the insulin receptor is itself a protein kinase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roth, R A -- Cassell, D J -- New York, N.Y. -- Science. 1983 Jan 21;219(4582):299-301.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6849137" target="_blank"〉PubMed〈/a〉
    Keywords: Adenosine Triphosphate/metabolism ; Cell Line ; Cells, Cultured ; Lymphocytes ; Molecular Weight ; Phosphoproteins/physiology ; Protein Kinases/*physiology ; Receptor, Insulin/*physiology
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  • 23
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    Recombination during gene transfer into mouse cells can restore the function of deleted genes (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-01-14
    Description: Two plasmids containing nonoverlapping deletions of the herpes simplex virus thymidine kinase gene were introduced into thymidine kinase-deficient mouse L cells by DNA-mediated gene transfer. Thymidine kinase-producing transformants were generated by a mixture of the two plasmids at a frequency significantly greater than that generated by either plasmid alone. Southern blot analyses demonstrated that functional thymidine kinase genes were generated by homologous recombination between the two deletion mutants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Small, J -- Scangos, G -- New York, N.Y. -- Science. 1983 Jan 14;219(4581):174-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6294829" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; Cells, Cultured ; Chromosome Deletion ; *Genetic Engineering ; Mice ; Mutation ; *Plasmids ; *Recombination, Genetic ; Simplexvirus ; Thymidine Kinase/*genetics
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  • 24
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    Human macrophages armed with murine immunoglobulin G2a antibodies to tumors destroy human cancer cells (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-08-26
    Description: Macrophages isolated from tumor-bearing patients as well as cultured human monocytes express Fc receptors that cross-react strongly with murine immunoglobulins of the G2a but only slightly or not at all with the G1, G2b, or G3 subclasses. Such macrophages in the presence of murine immunoglobulin G2a monoclonal antibodies to tumors mediated the killing of tumor cells in vitro. These data suggest that monoclonal antibodies of the G2a subclass may be useful in the immunotherapy of human cancer.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Steplewski, Z -- Lubeck, M D -- Koprowski, H -- CA-10815/CA/NCI NIH HHS/ -- CA-21124/CA/NCI NIH HHS/ -- CA-25874/CA/NCI NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1983 Aug 26;221(4613):865-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6879183" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibodies, Monoclonal/*immunology ; Cells, Cultured ; Cytotoxicity, Immunologic ; Humans ; *Immunity, Cellular ; Immunoglobulin G/immunology ; Immunotherapy ; Macrophages/*immunology ; Mice ; Monocytes/immunology ; Neoplasms, Experimental/immunology/therapy ; Receptors, Fc/*immunology ; Species Specificity
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  • 25
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    Hepatitis B virus infection in cultured human lymphoblastoid cells (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-08-12
    Description: Since it has been postulated that liver hepatocytes may become infected by hepatitis B virus (HBV) in vivo through direct contact with infected macrophages, the possibility that a circulating cell of hematopoietic origin might be susceptible to infection with HBV was investigated. Cells positive for HBV surface antigen were identified in aspirates of bone marrow cells from people infected with HBV. These cells were used to prepare a lymphoblastoid suspension culture that contains HBV-infected cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Romet-Lemonne, J L -- McLane, M F -- Elfassi, E -- Haseltine, W A -- Azocar, J -- Essex, M -- New York, N.Y. -- Science. 1983 Aug 12;221(4611):667-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6867736" target="_blank"〉PubMed〈/a〉
    Keywords: Cells, Cultured ; Hepatitis B/*microbiology/pathology ; Hepatitis B Surface Antigens/immunology ; Hepatitis B virus/growth & development ; Humans ; Liver/pathology ; Lymphocytes/*microbiology/pathology ; Male ; Middle Aged
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  • 26
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    Synaptic activity mediates death of hypoxic neurons (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-04-29
    Description: Cultured hippocampal neurons, when exposed to cyanide or an anoxic atmosphere in the early stages of differentiation, were not visibly affected. However, neurons in the mature cultures died when exposed to cyanide or anoxia. Cell death could be prevented by treatment with magnesium, which eliminates synaptic activity. These observations suggest that damage in hypoxic neurons is mediated by synaptic activity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rothman, S M -- 5 K07 N500568-02/PHS HHS/ -- New York, N.Y. -- Science. 1983 Apr 29;220(4596):536-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6836300" target="_blank"〉PubMed〈/a〉
    Keywords: Action Potentials/drug effects ; Animals ; Anoxia/*metabolism/physiopathology ; Cells, Cultured ; Hippocampus/cytology ; Magnesium/pharmacology ; Magnesium Chloride ; Membrane Potentials/drug effects ; Neurons/metabolism/*physiology ; Rats ; Sodium Cyanide/pharmacology ; Synapses/drug effects/*physiology
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  • 27
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    Infection of normal human epithelial cells by Epstein-Barr virus (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-03-11
    Description: Primary cultures of epithelial cells were grown from the tonsils and adenoids of patients with diseases not related to Epstein-Barr virus. The cells could not be infected by Epstein-Barr virus. Fluorescein-labeled Epstein-Barr virus and a cytofluorograph were then used to show that the epithelial cells do not have detectable receptors for the virus. However, implantation with Epstein-Barr virus receptors gave the cells the ability to bind the labeled virus. One to 5 percent of receptor-implanted cells exposed to the transforming B95-8 substrain of the virus expressed Epstein-Barr nuclear antigen. The early and viral capsid Epstein-Barr virus-determined antigens were not detected in the virus-infected cultures. The results show that normal human epithelial cells from the nasopharynx become susceptible to infection by Epstein-Barr virus when the membrane barrier resulting from the lack of viral receptors is overcome by receptor implantation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shapiro, I M -- Volsky, D J -- 1R01 CA33386-01/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1983 Mar 11;219(4589):1225-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6298935" target="_blank"〉PubMed〈/a〉
    Keywords: Cells, Cultured ; Epithelium/*microbiology ; Herpesviridae Infections/*microbiology ; Herpesvirus 4, Human/growth & development ; Humans ; Receptors, Virus/metabolism ; Virus Replication
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  • 28
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    Axonal proteins of presynaptic neurons during synaptogenesis (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-09-23
    Description: Changes occur in the synthesis and axonal transport of neuronal proteins in dorsal-root ganglia axons as a result of contact with cells from the spinal cord during synapse formation. Dorsal-root ganglia cells were cultured in a compartmental cel culture system that allows separate access to neuronal cell bodies and their axons. When cells from the ventral spinal cord were cultured with the dorsal-root ganglia axons, synapses were established within a few days. Metabolic labeling and two-dimensional electrophoresis revealed that four of more than 300 axonal proteins had changed in their expression by the time synapses were established. The highly selective nature of these changes suggests that the proteins involved may be important in the processes of axon growth and synapse formation and their regulation by the regional environment.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sonderegger, P -- Fishman, M C -- Bokoum, M -- Bauer, H C -- Nelson, P G -- New York, N.Y. -- Science. 1983 Sep 23;221(4617):1294-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6612344" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Axons/*metabolism ; Cells, Cultured ; Chick Embryo ; Isoelectric Point ; Molecular Weight ; Nerve Tissue Proteins/*biosynthesis ; Synapses/*metabolism
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  • 29
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    Latrunculins: novel marine toxins that disrupt microfilament organization in cultured cells (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-02-04
    Description: Two toxins, latrunculins A and B, which contain a new class of 16- and 14-membered marine macrolides attached to the rare 2-thiazolidinone moiety, were purified recently from the Red Sea sponge Latrunculia magnifica. The effects of these toxins on cultured mouse neuroblastoma and fibroblast cells have been evaluated. In both types of cells, submicromolar toxin concentrations rapidly induce striking changes in cell morphology that are reversible upon removal of the toxin. Immunofluorescence studies with antibodies specific for cytoskeletal proteins reveal that the toxins cause major alterations in the organization of microfilaments without obvious effects on the organization of the microtubular system.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Spector, I -- Shochet, N R -- Kashman, Y -- Groweiss, A -- New York, N.Y. -- Science. 1983 Feb 4;219(4584):493-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6681676" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/metabolism ; Animals ; *Bicyclo Compounds, Heterocyclic ; Cells, Cultured ; Cytoskeleton/*drug effects ; Fibroblasts/ultrastructure ; Marine Toxins/*pharmacology ; Microscopy, Fluorescence ; Microtubules/drug effects ; Neuroblastoma/ultrastructure ; Thiazoles/*pharmacology ; Thiazolidines
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  • 30
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    Phagocyte impotence caused by an invasive bacterial adenylate cyclase (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-09-03
    Description: For unknown reasons, humans infected with the bacterium Bordetella pertussis are exceptionally vulnerable to secondary infections. Bordetella species elaborate a soluble, heat-stable, and highly active adenylate cyclase. This enzyme is internalized by phagocytic cells and catalyzes the unregulated formation of adenosine 3',5'-monophosphate (cyclic AMP), thereby disrupting normal cellular function. This unusual phenomenon may explain Bordetella-induced aphylaxis and may prove to be useful for investigating a variety of cyclic AMP-governed processes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Confer, D L -- Eaton, J W -- 5T32H- L07062/PHS HHS/ -- New York, N.Y. -- Science. 1982 Sep 3;217(4563):948-50.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6287574" target="_blank"〉PubMed〈/a〉
    Keywords: Adenylyl Cyclases/*metabolism ; Animals ; Bordetella pertussis/*enzymology ; Cells, Cultured ; Cyclic AMP/biosynthesis ; Humans ; Macrophages/physiology ; Neutrophils/physiology ; Phagocytes/*physiology ; Rabbits ; Superoxides/metabolism ; Temperature
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  • 31
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    Fragment spanning the SV40 replication origin is the only DNA sequence required in cis for viral excision (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-12-17
    Description: A 311-base pair fragment containing the SV40 origin of replication was linked to the chicken thymidine kinase gene on a recombinant plasmid. This molecule was transfected into human 143 thymidine kinase-deficient (TK-) cells, and colonies positive for thymidine kinase were selected. When cell lines derived from these colonies were fused to permissive simian cells that produce SV40 T antigen, the recombinant plasmid excised itself from the human cellular genome and replicated with a high copy number per cell. These results show that this segment of the viral genome is the only sequence required in cis to mediate SV40 excision and replication upon fusion to permissive cells. In addition, we have shown that excised plasmids apparently identical to the input DNA can be efficiently rescued in Escherichia coli. SV40 excision and replication may therefore be useful for the recovery of cloned genes from eukaryotic cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Conrad, S E -- Liu, C P -- Botchan, M R -- CA 30490/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Dec 17;218(4578):1223-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6293055" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; Cells, Cultured ; Chickens ; *DNA Replication ; DNA, Viral/*genetics ; Gene Expression Regulation ; Genes, Viral ; Humans ; Recombination, Genetic ; Simian virus 40/*genetics ; *Virus Replication
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  • 32
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    Cellular transforming genes (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-08-27
    Description: Cellular genes potentially capable of inducing oncogenic transformation have been identified by homology to the transforming genes of retroviruses and by the biological activity of cellular DNA's in transfection assays. DNA's of various tumors induce transformation with high efficiencies, indicating that oncogenesis can involve dominant genetic alterations resulting in activation of cellular transforming genes. The identification and characterization of cellular transforming genes and their possible involvement in naturally occurring cancers, is discussed.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cooper, G M -- New York, N.Y. -- Science. 1982 Aug 27;217(4562):801-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6285471" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; *Cell Transformation, Neoplastic ; Cells, Cultured ; Chick Embryo ; DNA/genetics ; DNA Restriction Enzymes ; DNA, Viral/genetics ; Gene Expression Regulation ; *Genes ; Genes, Viral ; Humans ; Mice ; Neoplasms/*genetics ; Oncogene Protein pp60(v-src) ; Rats ; Retroviridae/*genetics ; Transfection ; Viral Proteins/genetics
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    Multiplication of tobacco mosaic virus in tobacco leaf disks is inhibited by (2'-5) oligoadenylate (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-06-25
    Description: The oligonucleotide (2'-5') oligoadenylate that is induced in interferon-treated animal cells protects plant tissue from infection by the tobacco mosaic virus. This inhibition of virus multiplication was obtained at concentrations comparable to those affecting protein synthesis and antiviral activities in animal cells. After treatment with (2'-5') oligoadenylate, the multiplicability of tobacco mosaic virus was reduced by 80 to 90 percent as measured by enzyme-linked immunosorbent assay. These results, along with the observation that human interferon protects tobacco tissue from infection by tobacco mosaic virus, indicate that plants and animals may have a common pathway for virus resistance.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Devash, Y -- Biggs, S -- Sela, I -- New York, N.Y. -- Science. 1982 Jun 25;216(4553):1415-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6178155" target="_blank"〉PubMed〈/a〉
    Keywords: Adenine Nucleotides/*pharmacology ; Animals ; Cells, Cultured ; Interferons/pharmacology ; Kinetics ; Oligonucleotides/*pharmacology ; Oligoribonucleotides/*pharmacology ; Plants, Toxic ; Tobacco/microbiology ; Tobacco Mosaic Virus/*drug effects ; Virus Replication/drug effects
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    Nucleotide sequence of the p21 transforming protein of Harvey murine sarcoma virus (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-09-03
    Description: Harvey murine sarcoma virus is a retrovirus which transforms cells by means of a single virally encoded protein called p21 has. We have determined the nucleotide sequence of 1.0 kilobase in the 5' half of the viral genome which encompasses the has coding sequences and its associated regulatory signals. The nucleotide sequence has identified the amino acid sequence of two additional overlapping polypeptides which share their reading frames and the carboxyl termini with p21 but which contain additional NH2-terminal amino acids.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dhar, R -- Ellis, R W -- Shih, T Y -- Oroszlan, S -- Shapiro, B -- Maizel, J -- Lowy, D -- Scolnick, E -- New York, N.Y. -- Science. 1982 Sep 3;217(4563):934-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6287572" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Base Sequence ; Cell Transformation, Viral ; Cells, Cultured ; Defective Viruses/*genetics ; Genes, Viral ; Oncogene Protein p21(ras) ; Peptide Fragments ; Protein Biosynthesis ; Protein Conformation ; RNA, Viral/genetics ; Sarcoma Viruses, Murine/*genetics ; Viral Proteins/analysis/*genetics
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    Secretion of newly taken-up ascorbic acid by adrenomedullary chromaffin cells (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-05-14
    Description: Primary cultures of bovine adrenomedullary cells accumulate carbon-14-labeled ascorbic acid through a saturable and energy-dependent process. The newly taken-up ascorbate is released concomitantly with catecholamines upon stimulation of chromaffin cell secretion. The release of ascorbate is Ca2+-dependent and mediated through activation of nicotinic receptors. These results indicate that exogenous ascorbate taken up into chromaffin cells is incorporated in situ into a secretable compartment, probably the catecholamine-containing chromaffin vesicles.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Daniels, A J -- Dean, G -- Viveros, O H -- Diliberto, E J Jr -- New York, N.Y. -- Science. 1982 May 14;216(4547):737-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7079733" target="_blank"〉PubMed〈/a〉
    Keywords: Adrenal Medulla/*secretion ; Animals ; Ascorbic Acid/metabolism/*secretion ; Biological Transport, Active ; Calcium/physiology ; Cattle ; Cells, Cultured ; Chromaffin Granules/metabolism ; Chromaffin System/*secretion ; Nicotine/pharmacology ; Receptors, Nicotinic/physiology ; Secretory Rate/drug effects
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    Placental mononuclear phagocytes as a source of interleukin-1 (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-10-29
    Description: Mouse and human placental tissue contains a large number of mononuclear phagocytes. These cells, isolated from placenta, were shown to produce the multifaceted immune factor interleukin-1. Activity in the supernatants of 48-hour mononuclear phagocyte cultures was associated with a 12,000- to 18,000-dalton protein, consistent with known interleukin-1 characteristics. Stimulation of phagocytosis with latex beads increased the production and release of interleukin-1 from these placental cells, which may be a useful source of this protein.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Flynn, A -- Finke, J H -- Hilfiker, M L -- CA 24474/CA/NCI NIH HHS/ -- CA 34107/CA/NCI NIH HHS/ -- RR 00210/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 1982 Oct 29;218(4571):475-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6981846" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Culture Media ; Humans ; Interleukin-1/*biosynthesis ; Interleukin-2/analysis ; Mice ; Phagocytes/*immunology ; Placenta/cytology/*immunology
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    Boradeption: a new procedure for transferring water-insoluble agents across cell membranes (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-07-09
    Description: A new process has been developed which is called "Boradeption" to signify boronic acid--dependent phase transfer of water-insoluble agents. Highly fluorescent boronic acid dervatives, FluoroBoras, are solubilized with a physiologically compatible carrier buffer containing a receptor group for boronate adduct formation. The system can be used to stain living cells. In another variation of the Boradeption concept, an insoluble reporter molecule containing a boronate receptor is solubilized with a carrier buffer containing a boronic acid functional group. The boronate-receptor complexes, which are in dynamic equilibrium, can be designed as vital stains and reagents for a variety of biological and medical applications.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gallop, P M -- Paz, M A -- Henson, E -- AG-00376-07/AG/NIA NIH HHS/ -- HL-20764-04A1/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1982 Jul 9;217(4555):166-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6178158" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biological Transport ; *Boron Compounds/therapeutic use ; *Boronic Acids/therapeutic use ; *Cell Membrane Permeability ; Cells, Cultured ; Chemical Phenomena ; Chemistry ; Chromogenic Compounds/metabolism ; Cricetinae ; Fibroblasts ; Fluorescent Dyes/metabolism ; Humans ; Rats ; Staining and Labeling
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    Active genes are sensitive to deoxyribonuclease I during metaphase (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-08-13
    Description: The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gazit, B -- Cedar, H -- Lerer, I -- Voss, R -- GM 20483/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1982 Aug 13;217(4560):648-50.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6283640" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Chromosomes, Human ; DNA/*genetics ; Deoxyribonuclease I ; Deoxyribonucleases/*pharmacology ; Endonucleases/*pharmacology ; Fibroblasts/metabolism ; Genes, Viral ; Humans ; Interphase ; Leukemia Virus, Murine/genetics ; *Metaphase ; Mice ; RNA, Viral/*genetics ; Transcription, Genetic
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    Insulin stimulates the phosphorylation of the 95,000-dalton subunit of its own receptor (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-01-08
    Description: Cultured human lymphocytes and rat hepatoma cells were labeled with [32P]orthophosphate and the insulin receptor subunits identified by immunoprecipitation and sodium dodecyl sulfate-gel electrophoreses. In both cell types the 95,000-dalton (beta) subunit of the insulin receptor was selectively phosphorylated. Phosphorylation was specifically stimulated by insulin in a dose-dependent fashion after 1 and 15 minutes of hormone treatment, whereas human growth hormone was without effect. This phosphorylation may be a very early event in insulin action.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kasuga, M -- Karlsson, F A -- Kahn, C R -- New York, N.Y. -- Science. 1982 Jan 8;215(4529):185-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7031900" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Growth Hormone/pharmacology ; Humans ; Insulin/*pharmacology ; Liver Neoplasms, Experimental/metabolism ; Lymphocytes ; Macromolecular Substances ; Molecular Weight ; Phosphorylation ; Rats ; Receptor, Insulin/*metabolism
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  • 40
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    Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-02-05
    Description: Gene transfer and immunoselection were used in the identification of a BALB/c genomic clone containing an H-2Ld gene (clone 27.5). Transformation of thymidine kinase-negative C3H mouse L cells with the cloned 27.5 DNA together with the herpes simplex virus tk gene produced transformants expressing Ld molecules detected by radioimmune assay with monoclonal hybridoma antibodies to Ld antigens. The foreign Ld gene products expressed by cloned mouse L cell transformants were shown to be virtually indistinguishable from BALB/c spleen Ld molecules by two-dimensional electrophoretic analysis of H-2Ld immunoprecipitates. These results indicate that the genomic clone 27.5 contains a functional BALB/c H-2Ld gene and demonstrate the usefulness of this approach for identifying the gene products encoded by cloned genes which are members of a multigene family. Furthermore, the ability to place cell-surface recognition molecules on the surfaces of foreign cells provides a powerful opportunity for functional analyses of these molecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goodenow, R S -- McMillan, M -- Orn, A -- Nicolson, M -- Davidson, N -- Frelinger, J A -- Hood, L -- CA 22662/CA/NCI NIH HHS/ -- CA 26199/CA/NCI NIH HHS/ -- GM 06965/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1982 Feb 5;215(4533):677-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7058331" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Genes ; H-2 Antigens/*genetics ; Isoelectric Point ; L Cells (Cell Line) ; Mice ; Mice, Inbred BALB C/*genetics ; Transformation, Genetic
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    Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-09-17
    Description: The biosynthesis of melanin is initiated by the catalytic oxidation of tyrosine to dopa by tyrosinase in a reaction that requires dopa as a cofactor. Tyrosine then catalyzes the dehydrogenation of dopa to dopaquinone. The subsequent reactions can proceed spontaneously in vitro. Tyrosinase, purified from murine melanomas and the skins of brown mice, has now been shown to catalyze a third reaction in mammalian melanogenesis, namely the conversion of 5,6-dihydroxyindile to melanochrome. This reaction requires dopa as a cofactor and is inhibited by tyrosine. Conversely, 5,6-dihydroxyindole inhibits the oxidation of tyrosine to dopa, so that the relative concentrations of tyrosine and 5,6-dihydroxyindole within the mammalian pigment cell are capable of regulating melanogenesis in a previously unrecognized fashion. Tyrosinase has the unusual property of catalyzing three distinct reactions within a single biochemical pathway: the hydroxylation of a monophenol, the dehydrogenation of a catechol, and the dehydrogenation of a dihydroxyindole. The first and third of these reactions require dopa as a cofactor; in the second reaction, dopa is a substrate.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Korner, A -- Pawelek, J -- DA-01147/DA/NIDA NIH HHS/ -- DA-05186/DA/NIDA NIH HHS/ -- New York, N.Y. -- Science. 1982 Sep 17;217(4565):1163-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6810464" target="_blank"〉PubMed〈/a〉
    Keywords: Catechol Oxidase/*metabolism ; Cells, Cultured ; Dihydroxyphenylalanine/metabolism ; Indoles/metabolism ; Kinetics ; Melanins/*biosynthesis ; Melanoma/enzymology ; Monophenol Monooxygenase/*metabolism ; Neoplasms, Experimental/enzymology ; Substrate Specificity ; Tyrosine/metabolism
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  • 42
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    Homologous regulation of gonadotropin-releasing hormone receptors in cultured pituitary cells (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-02-19
    Description: Specific receptors for gonadotropin-releasing hormone (GnRH) in cultured rat pituitary cells were increased by subnanomolar concentrations of GnRH agonists and decreased by high concentrations of these peptides. The antagonist [D-Phe2, Pro3, D-Phe6]GnRH did not alter GnRH binding capacity and blocked the increase in sites induced by GnRH. These findings provide direct evidence for the homologous regulation of GnRH receptors by physiological concentrations of the hypothalamic peptide, an action that could mediate the cyclical and postcastration increases in GnRH receptors and responsiveness of the pituitary gonadotrophs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Loumaye, E -- Catt, K J -- New York, N.Y. -- Science. 1982 Feb 19;215(4535):983-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6296998" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Dose-Response Relationship, Drug ; Feedback ; Female ; Gonadotropin-Releasing Hormone/analogs & derivatives/metabolism/pharmacology ; Pituitary Gland/secretion ; Pituitary Hormone-Releasing Hormones/*metabolism/pharmacology ; Rats ; Receptors, Cell Surface/*pharmacology ; Receptors, LHRH
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    Ornithine decarboxylase: essential in proliferation but not differentiation of human promyelocytic leukemia cells (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-04-02
    Description: The ornithine decarboxylase inhibitor DL-alpha-difluoromethyl ornithine inhibited a proliferation-associated increase in ornithine decarboxylase activity in cultured human promyelocytic leukemia cells, resulting in a marked suppression of cell proliferation and subsequent cell loss. It also inhibited increases in ornithine decarboxylase activity associated with the phorbol ester-induced conversion of promyelocytic HL-60 cells to monocyte-like cells and the retinoic acid-induced conversion to granulocyte-like cells. However, the inhibition of ornithine decarboxylase activity did not prevent cellular differentiation. These results suggest that polyamine biosynthesis has a specific role in cell proliferation rather than in inducing differentiation that is not accompanied by proliferation. The data also demonstrate that cessation of proliferation in HL-60 cells is not necessarily associated with differentiation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Luk, G D -- Civin, C I -- Weissman, R M -- Baylin, S B -- AM-27447/AM/NIADDK NIH HHS/ -- CA-18404/CA/NCI NIH HHS/ -- HL-19157/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1982 Apr 2;216(4541):75-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6950518" target="_blank"〉PubMed〈/a〉
    Keywords: Carboxy-Lyases/*physiology ; Cell Adhesion ; Cell Differentiation ; Cell Division ; Cells, Cultured ; Female ; Granulocytes/pathology ; Humans ; Leukemia, Myeloid, Acute/*enzymology/pathology ; Macrophages/pathology ; Ornithine Decarboxylase/*physiology ; Polyamines/biosynthesis ; Tetradecanoylphorbol Acetate/pharmacology ; Tretinoin/pharmacology
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    Tumor cell-platelet aggregation: induced by cathepsin B-like proteinase and inhibited by prostacyclin (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-08-06
    Description: The ability of tumor cells to metastasize may be related to their ability to promote aggregation of host platelets. The use of inhibitors of cysteine proteinases resulted in parallel inhibition of B16 amelanotic melanoma-induced platelet aggregation and of a cathepsin B activity. The antimetastatic agent prostacyclin inhibited platelet aggregation induced by the tumor cells and by papain, a cathepsin B-mimicking agent.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Honn, K V -- Cavanaugh, P -- Evens, C -- Taylor, J D -- Sloane, B F -- CA29405/CA/NCI NIH HHS/ -- CA29997/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Aug 6;217(4559):540-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7046053" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cathepsin B ; Cathepsins/*metabolism ; Cells, Cultured ; Cysteine Endopeptidases ; Endopeptidases/*metabolism ; Epoprostenol/*pharmacology ; Humans ; Melanoma/metabolism ; Mice ; Mice, Inbred C57BL ; Neoplasms, Experimental/metabolism ; Papain/pharmacology ; Platelet Aggregation/*drug effects ; Prostaglandins/*pharmacology ; Protease Inhibitors/pharmacology
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    Ti plasmids as gene carriers (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-06-18
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marx, J L -- New York, N.Y. -- Science. 1982 Jun 18;216(4552):1305.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7079762" target="_blank"〉PubMed〈/a〉
    Keywords: Cells, Cultured ; *Plant Tumors ; Plants/*genetics ; *Plasmids ; Rhizobium/genetics
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  • 46
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    A new treatment for burn victims (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-08-06
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Maugh, T H 2nd -- New York, N.Y. -- Science. 1982 Aug 6;217(4559):522.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7089578" target="_blank"〉PubMed〈/a〉
    Keywords: Adolescent ; Adult ; *Bandages ; *Biological Dressings ; Burns/*therapy ; Cells, Cultured ; Child ; Child, Preschool ; Epidermis/cytology ; Humans ; Surgical Flaps ; Wound Healing
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    Cysteamine: a potent and specific depletor of pituitary prolactin (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-07-30
    Description: Cysteamine rapidly reduces the concentration of prolactin in pituitary tissue in vivo and in vitro. The effect is dose-dependent, reversible, and cannot be accounted for by prolactin release. Cysteamine does not appear to exert its effect through dopamine receptors and does not alter lactotrope morphology, as determined by electron microscopy.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Millard, W J -- Sagar, S M -- Landis, D M -- Martin, J B -- AM 26252/AM/NIADDK NIH HHS/ -- New York, N.Y. -- Science. 1982 Jul 30;217(4558):452-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7089575" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Cysteamine/*pharmacology ; Domperidone/pharmacology ; Dose-Response Relationship, Drug ; Kinetics ; Male ; Pituitary Gland, Anterior/*metabolism ; Prolactin/analysis/*metabolism/secretion ; Rats ; Receptors, Dopamine/physiology ; Spiperone/pharmacology
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    Calcium and age in fibroblasts from control subjects and patients with cystic fibrosis (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-04-23
    Description: Intracellular calcium increases significantly as human fibroblasts age in culture. The calcium increase occurs 5 to 6 weeks (passages) earlier and is significantly greater in fibroblasts from subjects with cystic fibrosis in comparison with cells from control subjects. Intracellular calcium, which is thought to be a pathogenetic factor in cystic fibrosis, may also be a meaningful marker in cell aging.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shapiro, B L -- Lam, L F -- AG-02114/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 1982 Apr 23;216(4544):417-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7071590" target="_blank"〉PubMed〈/a〉
    Keywords: Adolescent ; Adult ; Calcium/*metabolism ; *Cell Survival ; Cells, Cultured ; Child ; Cystic Fibrosis/*metabolism/pathology ; Female ; Fibroblasts ; Humans ; Infant ; Male
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    Glucose stimulation of the antilipolytic effect of insulin in humans (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-06-03
    Description: Dose-response studies of the inhibition of lipolysis by insulin in isolated human adipocytes were conducted with the use of a sensitive bioluminescent assay of glycerol release. The addition of glucose to the incubation medium was associated with an increase in insulin sensitivity and an increase in the maximum insulin effect. The results suggest that glucose plays an important role in regulating the antilipolytic action of insulin in humans.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Arner, P -- Bolinder, J -- Ostman, J -- New York, N.Y. -- Science. 1983 Jun 3;220(4601):1057-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6342138" target="_blank"〉PubMed〈/a〉
    Keywords: Adipose Tissue/cytology ; Cells, Cultured ; Dose-Response Relationship, Drug ; Drug Synergism ; Glucose/*pharmacology ; Humans ; Insulin/*pharmacology ; Isoproterenol/pharmacology ; Lipolysis/*drug effects
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    Specific chromosome defect associated with human small-cell lung cancer; deletion 3p(14-23) (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-01-08
    Description: A specific, acquired chromosomal abnormality (deletion 3p) has been found in at least one chromosome 3 in 100 percent of the metaphases in 12 of 12 cell lines cultured from human small-cell lung cancer tissue and in 2-day tumor culture specimens from three patients. Analysis of the shortest region of overlap shows the deletion to be 3p(14-23). This specific change was not seen in five of five lung cancer cell lines other than small-cell lung cancer or in two lymphoblastoid lines cultured from cells of small-cell lung cancer patients whose tumors had the 3p deletion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Whang-Peng, J -- Kao-Shan, C S -- Lee, E C -- Bunn, P A -- Carney, D N -- Gazdar, A F -- Minna, J D -- New York, N.Y. -- Science. 1982 Jan 8;215(4529):181-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6274023" target="_blank"〉PubMed〈/a〉
    Keywords: Carcinoma, Small Cell/*genetics ; Cells, Cultured ; *Chromosome Deletion ; Chromosomes, Human, 1-3 ; Humans ; Karyotyping ; Lung Neoplasms/*genetics
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    High efficiency latency and activation of herpes simplex virus in human cells (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-09-17
    Description: Herpes simplex virus (HSV) exists in humans in a latent form that can be activated. To characterize the molecular basis of the cell-virus interactions and to analyze the state of the latent HSV genome, an in vitro model system was established. In this system a large fraction of the latently infected cells contain an HSV genome that can be activated. Cell survival was reduced minimally after repression of high multiplicity HSV type 1 (HSV-1) infection of human fibroblast cells with (E)-5-(2-bromovinyl)-2'-deoxyuridine in combination with human leukocyte interferon (IFN-alpha). A minimum of 1 to 3 percent of the surviving cells contained an HSV genome that could be activated either by human cytomegalovirus superinfection or reduction in incubation temperature.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wigdahl, B L -- Scheck, A C -- De Clercq, E -- Rapp, F -- CA 09124/CA/NCI NIH HHS/ -- CA 18450/CA/NCI NIH HHS/ -- CA 27503/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Sep 17;217(4565):1145-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6180477" target="_blank"〉PubMed〈/a〉
    Keywords: Bromodeoxyuridine/analogs & derivatives/therapeutic use ; Cells, Cultured ; Cytarabine/pharmacology ; Herpes Simplex/*physiopathology/therapy ; Humans ; Interferons/therapeutic use ; Simplexvirus/*physiology ; Virus Activation ; *Virus Replication/drug effects
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    Fluoride directly stimulates proliferation and alkaline phosphatase activity of bone-forming cells (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-10-21
    Description: Fluoride is one of the most potent but least well understood stimulators of bone formation in vivo. Bone formation was shown to arise from direct effects on bone cells. Treatment with sodium fluoride increased proliferation and alkaline phosphatase activity of bone cells in vitro and increased bone formation in embryonic calvaria at concentrations that stimulate bone formation in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Farley, J R -- Wergedal, J E -- Baylink, D J -- AM31061/AM/NIADDK NIH HHS/ -- AM31062/AM/NIADDK NIH HHS/ -- New York, N.Y. -- Science. 1983 Oct 21;222(4621):330-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6623079" target="_blank"〉PubMed〈/a〉
    Keywords: Alkaline Phosphatase/*metabolism ; Animals ; Bone Development/*drug effects ; Bone and Bones/*cytology/embryology/enzymology ; Cell Division/drug effects ; Cells, Cultured ; Chick Embryo ; Dose-Response Relationship, Drug ; Fluorides/*pharmacology ; Parathyroid Hormone/pharmacology
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    Cell growth on liquid microcarriers (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-03-25
    Description: Anchorage-dependent cell growth is demonstrated on microcarriers of fluorocarbon fluid formed by emulsification and stabilized with polylysine.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Keese, C R -- Giaever, I -- New York, N.Y. -- Science. 1983 Mar 25;219(4591):1448-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6828872" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Adhesion ; Cell Division ; *Cell Physiological Phenomena ; Cells, Cultured ; Culture Media ; Emulsions ; Fluorocarbons ; Kinetics
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    Substance P and somatostatin regulate sympathetic noradrenergic function (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-09-09
    Description: Peptidergic-noradrenergic interactions were examined in explants of rat sympathetic superior cervical ganglia and in cultures of dissociated cells. The putative peptide transmitters substance P and somatostatin each increased the activity of the catecholamine-synthesizing enzyme tyrosine hydroxylase after 1 week of exposure in culture. Maximal increases occurred at 10(-7) molar for each peptide, and either increasing or decreasing the concentration reduced the effects. Similar increases in tyrosine hydroxylase were produced by a metabolically stable agonist of substance P, while a substance P antagonist prevented the effects of the agonist. The data suggest that the increased tyrosine hydroxylase activity was mediated by peptide interaction with specific substance P receptors and that peptides may modulate sympathetic catecholaminergic function.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kessler, J A -- Adler, J E -- Black, I B -- New York, N.Y. -- Science. 1983 Sep 9;221(4615):1059-61.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6192502" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Bacitracin/pharmacology ; Captopril/pharmacology ; Cells, Cultured ; Culture Techniques ; Dose-Response Relationship, Drug ; Ganglia, Sympathetic/*enzymology ; Rats ; Somatostatin/*pharmacology ; Substance P/*pharmacology ; Tyrosine 3-Monooxygenase/*metabolism
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    Action potentials in macrophages derived from human monocytes (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-02-25
    Description: The electrical activity of macrophages derived from human blood monocytes was recorded in vitro with intracellular microelectrodes and was analyzed with computer-assisted data acquisition and analysis techniques. In cells impaled 6 to 8 days after the cultures were prepared, the resting potentials reached a maximum value of -72 millivolts. The cells were electrically excitable; spikes exhibited a slow upstroke, a fast downstroke, a discrete threshold, a large overshoot, and a brief undershoot. Repetitive firing was induced by a maintained depolarizing current. A positive relation was observed between transmembrane currents and resting potential. Voltage-current relations were nonrectifying for subthreshold current injections. Since these cells had not been treated with any specific activation factors, the electrical activity recorded is evidence for the presence of voltage-dependent inward and outward currents in the membranes of mature macrophages. The electrical signals generated by these cells may be useful for the assay of sensor and effector functions of macrophages, such as chemotaxis, receptor-ligand interactions, and phagocytosis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McCann, F V -- Cole, J J -- Guyre, P M -- Russell, J A -- AM0535/AM/NIADDK NIH HHS/ -- BRSG05392/RS/DRS NIH HHS/ -- CA17323/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1983 Feb 25;219(4587):991-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6823563" target="_blank"〉PubMed〈/a〉
    Keywords: Action Potentials ; Cell Differentiation ; Cells, Cultured ; Humans ; Macrophages/*physiology ; Monocytes/cytology
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    Plasmodium falciparum gametocytes from culture in vitro develop to sporozoites that are infectious to primates (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-09-10
    Description: Gametocytes of two strains of the human malaria parasite Plasmodium falciparum have been produced in high density by means of a continuous-flow cultivation system. The gametocytes of these two strains infected a mean of 36 percent and 71 percent, respectively, of Anopheles freeborni mosquitoes that fed on a suspension of red blood cells containing the culture gametocytes. Sporozoites harvested from the infected mosquito salivary glands were infective to the chimpanzee (Pan troglodytes) and the owl monkey (Aotus trivirgatus).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Campbell, C C -- Collins, W E -- Nguyen-Dinh, P -- Barber, A -- Broderson, J R -- New York, N.Y. -- Science. 1982 Sep 10;217(4564):1048-50.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7051285" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Anopheles/parasitology ; Aotus trivirgatus/parasitology ; Blood/parasitology ; Cells, Cultured ; Culture Media ; Humans ; Pan troglodytes/parasitology ; Plasmodium falciparum/*growth & development ; Salivary Glands/parasitology
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    Nerve fiber growth on defined hydrogel substrates (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-05-21
    Description: Cultured neurons become attached to hydrogel substrates prepared from 2-hydroxyethylmethacrylate but grow few nerve fibers unless fibronectin, collagen, or nerve growth factor is incorporated into the hydrogel. Antibodies to fibronectin inhibit nerve fiber growth on hydrogels containing fibronectin, which suggests that growing neurons interact directly with proteins trapped in the hydrogel. The adhesive requirements for attachment of neurons appear distinct and possibly less specific than those for fiber growth. Defined hydrogel substrates offer a controlled method for analyzing complex substrates that support nerve fiber growth and neuronal differentiation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Carbonetto, S T -- Gruver, M M -- Turner, D C -- 2S07RR0540220/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 1982 May 21;216(4548):897-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7079743" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Adhesion ; Cell Differentiation ; Cells, Cultured ; Chick Embryo ; Collagen/pharmacology ; Culture Media ; Fibronectins/pharmacology ; Nerve Growth Factors/pharmacology ; Neurons/*cytology ; *Polyhydroxyethyl Methacrylate ; *Polymethacrylic Acids
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    Induction of crisis forms in cultured Plasmodium falciparum with human immune serum from Sudan (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-06-11
    Description: Serums from 90 individuals from three areas in Sudan were tested for inhibitory activity against cultures of Plasmodium falciparum. In addition to inhibitory activity against merozoite invasion, all of the serums demonstrated, in varying degrees, the ability to retard intraerythrocyte development, leading to crisis forms and parasite deterioration. These retardation factors could be removed by absorption of immune serum with parasite-infected erythrocytes and were demonstrable in purified immunoglobulin fractions. Serum from donors in hypoendemic Khartoum did not retard parasite development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jensen, J B -- Boland, M T -- Akood, M -- AI-16312/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1982 Jun 11;216(4551):1230-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7043736" target="_blank"〉PubMed〈/a〉
    Keywords: Antibodies ; Antigens ; Cells, Cultured ; Erythrocytes/immunology/*parasitology ; Humans ; Immunity ; Malaria/*immunology ; Plasmodium falciparum/growth & development/*immunology/physiology ; Sudan
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    Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-10-29
    Description: Monoclonal antibodies specific for 5-bromodeoxyuridine have been produced and applied in detecting low levels of DNA replication on a cell-by-cell basis in vitro. The immunoglobulin-producing hybridomas were derived from spleen cells of mice immunized with a conjugate of iodouridine and ovalbumin. The cells were fused with the plasmacytoma line SP2/0Ag14. The antibodies produced are highly specific for bromodeoxyuridine and iododeoxyuridine and do not cross-react with thymidine. DNA synthesis in cultured cells exposed to bromodeoxyuridine for as short a time as 6 minutes can be detected easily and rapidly by an immunofluorescent staining method and quantitated by flow cytometry.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gratzner, H G -- 5R26CA-15480-09/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Oct 29;218(4571):474-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7123245" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibodies, Monoclonal ; Bromodeoxyuridine/*analysis/immunology ; Cells, Cultured ; *DNA Replication ; Flow Cytometry ; Idoxuridine/*analysis/immunology ; Mice
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    Expression of bovine leukemia virus genome is blocked by a nonimmunoglobulin protein in plasma from infected cattle (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-01-22
    Description: Plasma of cattle infected with bovine leukemia virus contains a soluble factor that blocks the expression of the viral genome in cultured lymphocytes. The blocking factor is not present in plasma of bovine leukemia virus-free cattle or of cattle infected with common bovine viruses. Blocking of bovine leukemia virus expression by the plasma factor is reversible, and seems to be mediated by a nonimmunoglobulin protein molecule.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gupta, P -- Ferrer, J F -- 3 PO1-CA14193/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Jan 22;215(4531):405-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6276975" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Blood Proteins/pharmacology ; Cattle ; Cattle Diseases/*blood ; Cells, Cultured ; Gene Expression Regulation/drug effects ; Leukemia/*blood ; Leukemia Virus, Bovine/*genetics ; Lymphocytes/microbiology ; Retroviridae/*genetics ; Viral Proteins/biosynthesis/genetics ; Virus Replication
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    Corticotropin-releasing activity of alpha-melanotropin (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-02-05
    Description: Synthetic alpha-melanotropin stimulated the release of immunoreactive adrenocorticotropin from primary cultures of rat anterior pituitary cells. The effect of the alpha-melanotropin was dose-dependent. Cells incubated with synthetic arginine-vasopressin and alpha-melanotropin simultaneously produced an amount of adrenocorticotropin that was greater than the sum of the amount that the cells produced in response to each peptide added separately. Other peptides structurally similar to alpha-melanotropin, such as, beta-, gamma 1-, gamma 2-, and gamma 3-melanotropin, were also tested for adrenocorticotropin-releasing activity. Only the gamma 3-melanotropin demonstrated a statistically significant effect. A vasopressin preparation (Pitressin, Parke-Davis) purified from posterior pituitaries and previously shown to contain some alpha-melanotropin was much more potent in releasing adrenocorticotropin than the synthetic vasopressin.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lis, M -- Julesz, J -- Gutkowska, J -- Genest, J -- New York, N.Y. -- Science. 1982 Feb 5;215(4533):675-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6276977" target="_blank"〉PubMed〈/a〉
    Keywords: Adrenocorticotropic Hormone/*secretion ; Animals ; Arginine Vasopressin/pharmacology ; Cells, Cultured ; Drug Synergism ; Hormones/pharmacology ; Melanocyte-Stimulating Hormones/*pharmacology ; Pituitary Gland/*secretion ; Rats ; Secretory Rate/drug effects
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    Viral receptors on isolated murine and human ependymal cells (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-01-22
    Description: Viruses that infect ependyma cause ependymitis in humans and hydrocephalus in experimental animals. We report that reovirus type 1 (which induces hydrocephalus in mice) binds to the surface of isolated human and murine ciliated ependymal cells. With the use of recombinant viral clones, the binding property was mapped to the type 1 viral hemagglutinin, which also determines in vivo the affinity of reovirus type 1 for ependyma. Mumps virus, measles virus, parainfluenza type 3, and herpes simplex virus type 1 bind to murine ependyma cells, whereas reovirus type 3, herpes simplex virus type 2, and poliovirus type 2 do not.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tardieu, M -- Weiner, H L -- 1K07-NS00237/NS/NINDS NIH HHS/ -- NSAI-16998/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1982 Jan 22;215(4531):419-21.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6276976" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Ependyma/*metabolism/microbiology ; Hemagglutinins, Viral/metabolism ; Humans ; Hydrocephalus/microbiology ; Measles virus/metabolism ; Mice ; Mumps virus/metabolism ; Parainfluenza Virus 3, Human/metabolism ; Receptors, Virus/*metabolism ; Reoviridae/*metabolism ; Simplexvirus/metabolism
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    Quantitative electron microscopic autoradiography of insulin, glucagon, and somatostatin binding sites on islets (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-09-17
    Description: After monolayer cultures of rat islets were exposed to [(125)I]insulin,[(125)I]glucagon, and [(125)I]tyrosinyl somatostatin, specific autoradiographic grains associated with each radioactively labeled ligand were found on B, A, and D cells. The density of labeling of the B, A, and D cells with each labeled ligand correlated well with the known actions of the three hormones on each of the islet cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Patel, Y C -- Amherdt, M -- Orci, L -- AM 21373/AM/NIADDK NIH HHS/ -- New York, N.Y. -- Science. 1982 Sep 17;217(4565):1155-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6126003" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Islets of Langerhans/cytology/*metabolism/ultrastructure ; Microscopy, Electron ; Rats ; Receptor, Insulin/*metabolism ; Receptors, Cell Surface/*metabolism ; Receptors, Glucagon ; Receptors, Somatostatin ; Somatostatin/metabolism
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    Cooperation between oncogenes. Investigators focus on cooperation between two or more oncogenes to help explain the multistep development of human cancers (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-11-11
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Marx, J L -- New York, N.Y. -- Science. 1983 Nov 11;222(4624):602-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6635658" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Cycle ; Cells, Cultured ; Mice ; *Oncogenes ; Platelet-Derived Growth Factor/*genetics
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    RNA splice site selection: evidence for a 5' leads to 3' scanning model (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-06-24
    Description: Human G gamma-globin genes containing tandem duplications of the donor (5') or acceptor (3') RNA splice sites of the second intervening sequence were constructed in order to ascertain the directionality of RNA splice site selection. These genes were introduced into cultured monkey cells, and their transcripts were analyzed. Transcripts of these duplication variants were spliced only at the proximal copy of the duplicated splice sites. These data are consistent with a 5' leads to 3' model of splice site selection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lang, K M -- Spritz, R A -- AM28598/AM/NIADDK NIH HHS/ -- New York, N.Y. -- Science. 1983 Jun 24;220(4604):1351-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6304877" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; Cells, Cultured ; Globins/genetics ; Haplorhini ; Humans ; Plasmids ; *RNA Splicing ; RNA, Messenger/genetics/physiology ; Simian virus 40/genetics ; Transcription, Genetic
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    Vasoactive intestinal peptide alters membrane potential and cyclic nucleotide levels in retinal horizontal cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-09-09
    Description: Vasoactive intestinal peptide stimulated the synthesis of adenosine 3',5'-monophosphate in fractions of isolated carp horizontal cells. When applied extracellularly to isolated and cultured horizontal cells, the peptide also induced a slow depolarization (30 to 40 millivolts) accompanied by a decrease in membrane resistance. However, analogs of adenosine 3',5'-monophosphate applied extracellularly or intracellularly, and forscolin applied extracellularly, had no effect on the membrane potential of cultured horizontal cells, indicating that the induced depolarization was not related to the accumulation of adenosine 3',5'-monophosphate in these cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lasater, E M -- Watling, K J -- Dowling, J E -- EY-00811/EY/NEI NIH HHS/ -- EY-00824/EY/NEI NIH HHS/ -- EY-05476/EY/NEI NIH HHS/ -- New York, N.Y. -- Science. 1983 Sep 9;221(4615):1070-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6308770" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Carps ; Cells, Cultured ; Cyclic AMP/*metabolism ; Dopamine/pharmacology ; Gastrointestinal Hormones/*pharmacology ; Kainic Acid/pharmacology ; Membrane Potentials/*drug effects ; Retina/*drug effects/physiology ; Vasoactive Intestinal Peptide/*pharmacology
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    Evidence for the recessive nature of cellular immortality (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-09-02
    Description: Fusion of immortal cell lines with normal human fibroblasts or certain other immortal cell lines yields hybrids having limited division potential. Cellular immortality was found to be a recessive phenotype in hybrids. It was also found that at least two separate events in the normal cell genome can result in immortality. In fusions involving certain immortal parent cells, these events can be complemented to result in hybrids with finite division capacity.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pereira-Smith, O M -- Smith, J R -- AG 03262/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 1983 Sep 2;221(4614):964-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6879195" target="_blank"〉PubMed〈/a〉
    Keywords: *Cell Division ; Cell Line ; *Cell Survival ; Cells, Cultured ; Genes, Recessive ; Humans ; Hybrid Cells/*physiology ; Phenotype
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    Activation of 2-aminofluorene by cultured plant cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-03-25
    Description: Cultured tobacco plant cells activated 2-aminofluorene to an agent mutagenic to Salmonella typhimurium strain TA98. The plant activation of 2-aminofluorene is heat-inactivated and may not involve solely cytochrome P-450. The kinetics of activation demonstrated both time- and concentration-dependent responses.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Plewa, M J -- Weaver, D L -- Blair, L C -- Gentile, J M -- ES02384/ES/NIEHS NIH HHS/ -- New York, N.Y. -- Science. 1983 Mar 25;219(4591):1427-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6338591" target="_blank"〉PubMed〈/a〉
    Keywords: Biotransformation ; Cells, Cultured ; Fluorenes/*metabolism/pharmacology ; Kinetics ; Mutagenicity Tests ; Mutagens/*metabolism ; *Mutation ; *Plants, Toxic ; Salmonella typhimurium/drug effects ; Tobacco/*metabolism
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    Isolation and transmission of human retrovirus (human t-cell leukemia virus) (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-02-18
    Description: Nine new isolates of human T-cell leukemia-lymphoma virus (HTLV) were obtained from cells of seven patients with malignancies of mature T cells and from two clinically normal relatives of a T-cell leukemia patient. These people were from the United States, Israel, the West Indies, and Japan. The virus was detected in the fresh T cells and was isolated from the established T-cell lines. Each isolate is closely related to the first HTLV isolate, and all the new HTLV isolates were transmitted into normal human T cells obtained from the umbilical cord blood of newborns.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Popovic, M -- Sarin, P S -- Robert-Gurroff, M -- Kalyanaraman, V S -- Mann, D -- Minowada, J -- Gallo, R C -- New York, N.Y. -- Science. 1983 Feb 18;219(4586):856-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6600519" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Line ; Cells, Cultured ; Female ; Humans ; Leukemia/*microbiology ; Male ; Retroviridae/growth & development/*isolation & purification ; T-Lymphocytes/*microbiology
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    Mobilization of cellular calcium-45 and lead-210: effect of physiological stimuli (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-04-15
    Description: Isolated rat hepatocytes in primary culture were used as a model system to evaluate the effects of selected hormones and culture conditions on the efflux of calcium-45 and lead-210 from cells labeled with these isotopes. Alpha-adrenergic stimuli, angiotensin, vasopressin, dibutyryl adenosine 3',5'-monophosphate, and reduced phosphate concentrations in the medium increased the efflux of calcium-45 and lead-210. Glucagon and insulin had no effect, but increased phosphate concentrations decreased the efflux of both isotopes. Experiments with hepatocytes cultured in a medium free of calcium and lead demonstrated that the increased efflux of calcium-45 and lead-210 induced by hormones was the result of mobilization of the ions from intracellular stores. The data indicate that the physiological stimuli that mobilized calcium ions also mobilized lead ions, and that the mobilized lead would be available to interact with calcium-mediated cell functions.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Pounds, J G -- Mittelstaedt, R A -- New York, N.Y. -- Science. 1983 Apr 15;220(4594):308-10.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6301003" target="_blank"〉PubMed〈/a〉
    Keywords: Angiotensin II/pharmacology ; Animals ; Bucladesine/pharmacology ; Calcium Radioisotopes/*metabolism ; Cells, Cultured ; Epinephrine/pharmacology ; Insulin/pharmacology ; Lead/*metabolism ; Liver/cytology ; Phosphates/pharmacology ; Propranolol/pharmacology ; Radioisotopes ; Rats ; Vasopressins/pharmacology
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    Irreversible ligands with high selectivity toward delta and mu opiate receptors (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-04-15
    Description: Alkylating agents that display strong selectivity for opiate receptor types delta or mu were prepared by appropriate modification of the structures of the strong analgesics fentanyl, etonitazene, and endoethenotetrahydrooripavine. The availability of these substances should facilitate studies of the structural basis of receptor specificity and of the physiologic roles of these receptors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rice, K C -- Jacobson, A E -- Burke, T R Jr -- Bajwa, B S -- Streaty, R A -- Klee, W A -- New York, N.Y. -- Science. 1983 Apr 15;220(4594):314-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6132444" target="_blank"〉PubMed〈/a〉
    Keywords: Alkylation ; Animals ; Benzimidazoles/analogs & derivatives/metabolism ; Brain/physiology ; Cells, Cultured ; Chemical Phenomena ; Chemistry ; Enkephalin, Methionine/analogs & derivatives/metabolism ; Fentanyl/analogs & derivatives/metabolism ; *Isothiocyanates ; Ligands ; Rats ; Receptors, Opioid/*metabolism/physiology ; Thebaine/analogs & derivatives/pharmacology
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    Modulation of the metastatic capability in B16 melanoma by cell shape (1983)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1983-09-23
    Description: The lung colonization of B16-F1 cells grown in flat and spherical configurations was studied. Cells cultivated in vitro as spheroids on a nonadhesive substrate expressed in a reversible fashion a marked increase in their propensity to establish metastases. The altered metastatic capability was accompanied by a reversible reduction in the accessibility of cell surface proteins to external iodination and by a dramatic decrease in the synthesis of vimentin.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Raz, A -- Ben-Ze'ev, A -- New York, N.Y. -- Science. 1983 Sep 23;221(4617):1307-10.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6612347" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Adhesion ; Cell Division ; Cells, Cultured ; Intermediate Filament Proteins/biosynthesis ; Lung Neoplasms/secondary ; Melanoma/*pathology ; Membrane Proteins/physiology ; Mice ; *Neoplasm Metastasis ; Neoplasm Proteins/physiology ; Neoplasms, Experimental/pathology ; Vimentin
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    DNA strand breakage in human leukocytes exposed to a tumor promoter, phorbol myristate acetate (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-03-05
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Birnboim, H C -- New York, N.Y. -- Science. 1982 Mar 5;215(4537):1247-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6276978" target="_blank"〉PubMed〈/a〉
    Keywords: Cell Survival/drug effects ; Cells, Cultured ; Cocarcinogenesis ; *Dna ; Free Radicals ; Humans ; Kinetics ; Leukocytes/*drug effects ; Oxygen Consumption/drug effects ; Phorbols/*pharmacology ; Superoxides/metabolism ; Tetradecanoylphorbol Acetate/*pharmacology
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    Bleb formation in hepatocytes during drug metabolism is caused by disturbances in thiol and calcium ion homeostasis (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-09-24
    Description: A wide variety of toxic chemicals cause blebbing of the plasma membrane in isolated hepatocytes. These alterations in surface structure occur well before cell death. The formation of blebs appears to be directly related to changes in the concentration of extramitochondrial calcium ions. These changes probably reduce the ability of the hepatocyte cytoskeleton to maintain normal surface morphology. The concentration of soluble thiols, notably glutathione, appears to regulate the size of the extramitochondrial calcium ion pool. Disturbances in intracellular thiol and calcium ion homeostasis therefore seem to be responsible for the surface blebbing observed during toxic injury to isolated hepatocytes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Jewell, S A -- Bellomo, G -- Thor, H -- Orrenius, S -- Smith, M -- New York, N.Y. -- Science. 1982 Sep 24;217(4566):1257-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7112127" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Calcium/*physiology ; Cell Membrane/*physiology ; Cells, Cultured ; Cytoplasm/physiology ; Glutathione/physiology ; Homeostasis ; Liver/*drug effects/physiology/ultrastructure ; Male ; Mitochondria, Liver/physiology ; Rats ; Sulfhydryl Compounds/*physiology
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    Is the acetylcholine receptor a rabies virus receptor? (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-01-08
    Description: Rabies virus was found on mouse diaphragms and on cultured chick myotubes in a distribution coinciding with that of the acetylcholine receptor. Treatment of the myotubes with alpha-bungarotoxin and d-tubocurarine before the addition of the virus reduced the number of myotubes that became infected with rabies virus. These findings together suggest that acetylcholine receptors may serve as receptors for rabies virus. The binding of virus to acetylcholine receptors, which are present in high density at the neuromuscular junction, would provide a mechanism whereby the virus could be locally concentrated at sites in proximity to peripheral nerves facilitating subsequent uptake and transfer to the central nervous system.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lentz, T L -- Burrage, T G -- Smith, A L -- Crick, J -- Tignor, G H -- AI 11132/AI/NIAID NIH HHS/ -- AI 12541/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1982 Jan 8;215(4529):182-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7053569" target="_blank"〉PubMed〈/a〉
    Keywords: Acetylcholine/metabolism ; Acetylcholinesterase/metabolism ; Animals ; Binding, Competitive ; Bungarotoxins/metabolism ; Cells, Cultured ; Chick Embryo ; Diaphragm ; Mice ; Microscopy, Electron ; Neuromuscular Junction/*metabolism ; Rabies virus/*metabolism ; Receptors, Cholinergic/*metabolism ; Receptors, Virus/*metabolism ; Tubocurarine/metabolism
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    Monoclonal antibodies to hypothalamic growth hormone-releasing factor with picomoles of antigen (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-11-26
    Description: Monoclonal antibodies specific for rat hypothalamic growth hormone-releasing factor (rGRF) have been produced by in vitro immunization of mouse spleen cells with less than 1 nanomole of rGRF in a partially purified preparation. Hybridoma supernatants were screened for anti-rGRF activity by use of a pituitary culture assay system that can detect growth hormone-releasing factor in the femtomole range. Such highly sensitive in vitro techniques permit the use of picomole quantities of an antigen in partially purified preparations for the isolation of monoclonal antibodies, which can in turn be used in biological studies and in immunochemical procedures for large-scale purification and isolation of that antigen.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Luben, R A -- Brazeau, P -- Bohlen, P -- Guillemin, R -- AD 09690-07/AD/ADAMHA HHS/ -- AM 18811-07/AM/NIADDK NIH HHS/ -- DE 00057/DE/NIDCR NIH HHS/ -- New York, N.Y. -- Science. 1982 Nov 26;218(4575):887-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6813967" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibodies, Monoclonal/*immunology ; Antibody Specificity ; Cells, Cultured ; Dose-Response Relationship, Immunologic ; Growth Hormone-Releasing Hormone/*immunology ; Hybridomas/*immunology ; Mice ; Rats
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    A new tumor-promoting agent, dihydroteleocidin B, markedly enhances chemically induced malignant cell transformation (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-04-30
    Description: Teleocidin, which was isolated from mycelia of Streptomyces, is a potent tumor promoter in mouse skin. The catalytically hydrogenated compound dihydroteleocidin B markedly enhanced malignant cell transformation induced by 3-methylcholanthrene or ultraviolet radiation. Dihydroteleocidin B was at least 100 times more effective in enhancing transformation than 12-O-tetradecanoyl phorbol-13-acetate, the strongest promoter known until now, whereas both promoters showed equal capacities to induce early membrane effects and DNA synthesis.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hirakawa, T -- Kakunaga, T -- Fujiki, H -- Sugimura, T -- New York, N.Y. -- Science. 1982 Apr 30;216(4545):527-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6280280" target="_blank"〉PubMed〈/a〉
    Keywords: Alkaloids/*pharmacology ; Animals ; Carcinogens/*pharmacology ; Cell Division/drug effects ; Cell Transformation, Neoplastic/*drug effects ; Cells, Cultured ; Epidermal Growth Factor/metabolism ; *Lyngbya Toxins ; Methylcholanthrene ; Mice ; Receptor, Epidermal Growth Factor ; Receptors, Cell Surface/metabolism
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    New focus on replacing animals in the lab (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-01-01
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Holden, C -- New York, N.Y. -- Science. 1982 Jan 1;215(4528):35-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7053557" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Animals, Laboratory ; Cells, Cultured ; Legislation as Topic ; Research Design
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    Human cell hybrids: analysis of transformation and tumorigenicity (1982)
    American Association for the Advancement of Science (AAAS)
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    Publication Date: 1982-01-15
    Description: Intraspecific human-human cell hybrids provide a stable model system with which to investigate the genetic control of transformed and tumorigenic phenotypes. Using this system it has been shown that these phenotypes are under separate genetic control. Furthermore, the tumorigenic phenotype can be complemented by fusion of different tumorigenic cells, resulting in nontumorigenic hybrids. This system also provides information on the control of differentiated function. Molecular cytogenetic techniques should reveal the nature of the chromosomal control of neoplastic transformation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stanbridge, E J -- Der, C J -- Doersen, C J -- Nishimi, R Y -- Peehl, D M -- Weissman, B E -- Wilkinson, J E -- CA09054/CA/NCI NIH HHS/ -- CA19401/CA/NCI NIH HHS/ -- GM07134/GM/NIGMS NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1982 Jan 15;215(4530):252-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7053574" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Division ; Cell Transformation, Neoplastic/*pathology ; Cell Transformation, Viral ; Cells, Cultured ; Fibronectins/metabolism ; Humans ; *Hybrid Cells/pathology ; Karyotyping ; Mice ; Mice, Nude ; Neoplasms/*genetics/pathology ; Neoplasms, Experimental/pathology ; Phenotype
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    Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-09-03
    Description: The transforming protein of Kirsten murine sarcoma virus (Ki-MuSV) is a virally encoded 21-kilodalton protein called p21 kis. The sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned Ki-MuSV DNA to induce focal transformation of mouse cells. Nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 567 nucleotides coding for p21 kis protein.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tsuchida, N -- Ryder, T -- Ohtsubo, E -- CA-22701/CA/NCI NIH HHS/ -- CA21124/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1982 Sep 3;217(4563):937-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6287573" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Base Sequence ; Cell Transformation, Viral ; Cells, Cultured ; DNA Restriction Enzymes ; DNA, Recombinant ; DNA, Viral/genetics ; Genes, Viral ; Kirsten murine sarcoma virus/*genetics ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Mutation ; Oncogene Protein p21(ras) ; RNA, Viral/genetics ; Sarcoma Viruses, Murine/*genetics ; Viral Proteins/*genetics
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    Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS) (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-05-20
    Description: A retrovirus belonging to the family of recently discovered human T-cell leukemia viruses (HTLV), but clearly distinct from each previous isolate, has been isolated from a Caucasian patient with signs and symptoms that often precede the acquired immune deficiency syndrome (AIDS). This virus is a typical type-C RNA tumor virus, buds from the cell membrane, prefers magnesium for reverse transcriptase activity, and has an internal antigen (p25) similar to HTLV p24. Antibodies from serum of this patient react with proteins from viruses of the HTLV-I subgroup, but type-specific antisera to HTLV-I do not precipitate proteins of the new isolate. The virus from this patient has been transmitted into cord blood lymphocytes, and the virus produced by these cells is similar to the original isolate. From these studies it is concluded that this virus as well as the previous HTLV isolates belong to a general family of T-lymphotropic retroviruses that are horizontally transmitted in humans and may be involved in several pathological syndromes, including AIDS.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Barre-Sinoussi, F -- Chermann, J C -- Rey, F -- Nugeyre, M T -- Chamaret, S -- Gruest, J -- Dauguet, C -- Axler-Blin, C -- Vezinet-Brun, F -- Rouzioux, C -- Rozenbaum, W -- Montagnier, L -- New York, N.Y. -- Science. 1983 May 20;220(4599):868-71.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6189183" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/*microbiology ; Adult ; Animals ; Antibodies, Viral/immunology ; Cells, Cultured ; Humans ; Male ; Microscopy, Electron ; RNA-Directed DNA Polymerase/metabolism ; Retroviridae/*isolation & purification ; T-Lymphocytes/microbiology ; Tumor Virus Infections/*microbiology
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 82
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    Myasthenic globulin enhances the loss of acetylcholine receptor clusters (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-01-14
    Description: Acetylcholine receptors are present in the sarcolemma of cultured skeletal muscle myotubes either as large clusters or in a diffuse distribution. Both the clustered and diffuse acetylcholine receptors are potentially removable from the membrane. Treatment of myotubes with globulin from patients with myasthenia gravis causes the loss of acetylcholine receptor clusters and the concomitant appearance of acetylcholine receptor microaggregates. The rate of acetylcholine receptor cluster loss is greater than the rate of acetylcholine receptor degradation, indicating that acetylcholine receptors are disrupted from clusters to form microaggregates before being removed from the plasma membrane.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bursztajn, S -- McManaman, J L -- Elias, S B -- Appel, S H -- New York, N.Y. -- Science. 1983 Jan 14;219(4581):195-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6849132" target="_blank"〉PubMed〈/a〉
    Keywords: *Autoantibodies ; Cells, Cultured ; Humans ; Immunologic Capping ; Macromolecular Substances ; Membrane Proteins/metabolism ; Myasthenia Gravis/*immunology ; Pinocytosis ; Receptors, Cholinergic/immunology/*metabolism
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 83
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    Sodium currents in segments of human heart cells (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-04-15
    Description: Isolated human heart cells were partially drawn into the lumen of a plastic tube and cleaved at the partitioning tube wall by intraluminal suction pulses. The extraluminal segment (10 to 20 percent of the cell length) was suitable for intracellular perfusion and voltage clamp. The time and voltage dependence of the sodium current, and the responses to changes in driving force and channel blockers, illustrate the potential of these preparations as models for the study of membrane channels.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bustamante, J O -- McDonald, T F -- New York, N.Y. -- Science. 1983 Apr 15;220(4594):320-1.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6301004" target="_blank"〉PubMed〈/a〉
    Keywords: Cells, Cultured ; Humans ; Ion Channels/drug effects ; Lidocaine/pharmacology ; Membrane Potentials/drug effects ; Myocardium/*cytology/metabolism ; Sodium/*metabolism/physiology ; Tetrodotoxin/pharmacology
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 84
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    Human brain tumor--derived cell lines: growth rate reduced by human fibroblast interferon (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-02-18
    Description: The biological response modifier human beta-interferon had pronounced antigrowth effects on various histologic types of human brain tumor cells but no effects on a nontransformed cell line, MRC-5. The cultures of brain tumor cells showed severe alterations indicative of cell injury and death after exposure to beta-interferon for 2 to 6 days. Similar results were obtained with cells freshly explanted from human brain tumors. The results indicate that it may be possible to use fresh, explanted tumor tissue to identify patients who might benefit from therapy with beta-interferon.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cook, A W -- Carter, W A -- Nidzgorski, F -- Akhtar, L -- New York, N.Y. -- Science. 1983 Feb 18;219(4586):881-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6401866" target="_blank"〉PubMed〈/a〉
    Keywords: Brain Neoplasms/pathology/*therapy ; Cell Division ; Cells, Cultured ; Humans ; Interferon-gamma/pharmacology/*therapeutic use
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 85
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    Cell-to-cell transfer of interferon-induced antiproliferative activity (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-09-02
    Description: Interferon-treated cells rapidly and efficiently transferred the antiproliferative activity of interferon to untreated cells. This phenomenon was not due to the carry-over of interferon by the interferon-treated cells. Thus, to evoke an antiproliferative state, interferon did not directly contact each cell in a population. The results suggest a novel mechanism by which interferon may indirectly regulate cell growth, and suggests that cells other than those of the immune system may play a role in controlling tumor growth in tissue where cell-to-cell contact occurs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lloyd, R E -- Blalock, J E -- Stanton, G J -- 03348/PHS HHS/ -- AM 30046/AM/NIADDK NIH HHS/ -- New York, N.Y. -- Science. 1983 Sep 2;221(4614):953-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6192500" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Cell Communication ; Cell Division/*drug effects ; Cells, Cultured ; Humans ; Interferons/*pharmacology ; Leukemia L1210 ; Mice ; Species Specificity
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 86
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    Serum factor supporting long-term survival of rat central neurons in culture (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-06-24
    Description: Gel filtration of serum at pH 3.6 yielded a fraction that supported long-term (months) survival of dissociated rat central neurons in monolayer culture more reliably than the traditionally used unfractionated serum. The cultures remained neuron-rich, because this fraction did not support the proliferation of glia and fibroblasts that occurs in whole serum. With an apparent molecular weight of 55,000 and an isoelectric point of 5.6, the active factor (or factors) in this fraction is distinct from any well-defined growth factor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kaufman, L M -- Barrett, J N -- NS07044/NS/NINDS NIH HHS/ -- NS12207/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1983 Jun 24;220(4604):1394-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6857258" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cattle ; *Cell Survival/drug effects ; Cells, Cultured ; Chromatography, Gel ; Horses ; Isoelectric Focusing ; Molecular Weight ; Nerve Growth Factors/isolation & purification/*pharmacology ; Neurons/drug effects/*physiology ; Rats ; Rats, Inbred Strains ; Spinal Cord/cytology
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 87
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    Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-10-14
    Description: Oncogenes capable of transforming NIH/3T3 cells are often present in human tumors and tumor cell lines. Such oncogenes were not detected in normal fibroblast lines derived from patients with several clinical syndromes associated with greatly increased cancer risk. Thus, germ-line transmission of these oncogenes does not appear to be the predisposing factor responsible for these high cancer risk syndromes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Needleman, S W -- Yuasa, Y -- Srivastava, S -- Aaronson, S A -- New York, N.Y. -- Science. 1983 Oct 14;222(4620):173-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6623066" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Transformation, Neoplastic/*pathology ; Cells, Cultured ; DNA, Neoplasm/*genetics ; Gardner Syndrome/genetics ; Humans ; Mice ; *Oncogenes ; Precancerous Conditions/*genetics ; Risk ; Skin/pathology
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  • 88
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    Modulation of synapse formation by cyclic adenosine monophosphate (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-11-18
    Description: Synapses between neuroblastoma-hybrid cells and myotubes exhibit a high degree of plasticity. Increase of cyclic adenosine monophosphate (AMP) levels of the hybrid cells for several days results in the appearance of functional voltage-sensitive Ca2+ channels, which are required for evoked secretion of acetylcholine. The results show that cyclic AMP regulates synaptogenesis by regulating the expression of voltage-sensitive Ca2+ channels, and suggest that cyclic AMP affects posttranslational modifications of some glycoproteins and cellular levels of certain proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Nirenberg, M -- Wilson, S -- Higashida, H -- Rotter, A -- Krueger, K -- Busis, N -- Ray, R -- Kenimer, J G -- Adler, M -- New York, N.Y. -- Science. 1983 Nov 18;222(4625):794-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6314503" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibodies, Monoclonal ; Calcium/physiology ; Cell Adhesion ; Cells, Cultured ; Cyclic AMP/*physiology ; Gene Expression Regulation ; Humans ; Membrane Potentials ; Nerve Tissue Proteins/physiology ; Neuromuscular Junction/*physiology ; Neuronal Plasticity ; Receptors, Cell Surface/physiology ; Retina/*physiology ; Synapses/*physiology
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  • 89
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    Tumor suppression with a combination of alpha-difluoromethyl ornithine and interferon (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-02-18
    Description: alpha-Difluoromethyl ornithine and mouse type 1 interferon, when administered simultaneously, were highly toxic to B16 melanoma cells in culture. Oral administration of alpha-difluoromethyl ornithine suppressed B16 melanoma development in mice 85 percent whereas interferon given subcutaneously inhibited tumor growth only 24 percent. Total or near total suppression of tumor growth was observed in mice receiving both treatments.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sunkara, P S -- Prakash, N J -- Mayer, G D -- Sjoerdsma, A -- New York, N.Y. -- Science. 1983 Feb 18;219(4586):851-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6186025" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Eflornithine ; Interferons/*administration & dosage ; Melanoma/therapy ; Mice ; Neoplasms, Experimental/*therapy ; Ornithine/administration & dosage/*analogs & derivatives ; Ornithine Decarboxylase Inhibitors
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 90
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    Immunocytochemically identified vasopressin neurons in culture show slow, calcium-dependent electrical responses (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-09-09
    Description: From morphological characterization and intracellular recordings, monolayer cultures derived from fetal mouse hypothalami were found to include functionally differentiated peptide neurons, a number of which appear to contain vasopressin. These cells exhibited particular patterns of slow, calcium-dependent membrane depolarizations, resembling in their periodicity and duration the phasic activity of vasopressin neurons recorded extracellularly in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Theodosis, D T -- Legendre, P -- Vincent, J D -- Cooke, I -- New York, N.Y. -- Science. 1983 Sep 9;221(4615):1052-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6348947" target="_blank"〉PubMed〈/a〉
    Keywords: Action Potentials ; Animals ; Calcium/*pharmacology ; Cells, Cultured ; *Electrophysiology ; Histocytochemistry ; Hypothalamus/analysis/*cytology ; Immunologic Techniques ; Mice ; Neurons/analysis ; Vasopressins/*analysis
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 91
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    alpha-Difluoromethylornithine-induced polyamine depletion of 9L tumor cells modifies drug-induced DNA cross-link formation (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-12-09
    Description: Depletion of intracellular levels of polyamines, which are believed to have a role in the intranuclear stabilization of DNA, alters the cytotoxicity of 1,3-bis(2-chloroethyl)-1-nitrosourea and cis-diamminedichloroplatinum II in 9L rat brain tumor cells. Alkaline elution techniques were used to show that polyamine depletion alters the number of DNA cross-links formed by these cytotoxic agents.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tofilon, P J -- Deen, D F -- Marton, L J -- CA-09215/CA/NCI NIH HHS/ -- CA-13525/CA/NCI NIH HHS/ -- CA-31867/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1983 Dec 9;222(4628):1132-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6417790" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Carmustine/*pharmacology ; Cells, Cultured ; Cisplatin/*pharmacology ; Cross-Linking Reagents ; *DNA/radiation effects ; Eflornithine ; Ornithine/*analogs & derivatives/pharmacology ; Ornithine Decarboxylase Inhibitors ; Polyamines/antagonists & inhibitors ; Rats
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 92
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    Measurement of suppressor transfer RNA activity (1983)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1983-08-26
    Description: Transfer RNA (tRNA) suppression of nonsense mutations in prokaryotic systems has been widely used to study the structure and function of different prokaryotic genes. Through genetic engineering techniques, it is now possible to introduce suppressor (Su+) tRNA molecules into mammalian cells. A quantitative assay of the suppressor tRNA activity in these mammalian cells is described; it is based on the amount of tRNA-mediated readthrough of a terminating codon in the influenza virus NS1 gene after the cells are infected with virus. Suppressor activity in L cells continuously expressing Su+ (tRNAtyr) was 3.5 percent and that in CV-1 cells infected with an SV40- Su+ (tRNAtyr) recombinant was 22.5 percent.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Young, J F -- Capecchi, M -- Laski, F A -- RajBhandary, U L -- Sharp, P A -- Palese, P -- AI-11823/AI/NIAID NIH HHS/ -- AI-18998/AI/NIAID NIH HHS/ -- GM17151/GM/NIGMS NIH HHS/ -- etc. -- New York, N.Y. -- Science. 1983 Aug 26;221(4613):873-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6308765" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Eukaryotic Cells/physiology ; Genes, Viral ; Mice ; Orthomyxoviridae/genetics ; Peptide Chain Termination, Translational ; Protein Biosynthesis ; RNA, Transfer/*genetics ; Simian virus 40/genetics ; *Suppression, Genetic
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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