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  • Lepidoptera  (112)
  • Immunocytochemistry  (73)
  • Drosophila
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  • 1
    Digitale Medien
    Digitale Medien
    Etude électroantennographique de la réception de stimulus odorants d'origine végétale chez Acrolepiopsis assectella (1986)
    Springer
    Entomologia experimentalis et applicata 40 (1986), S. 13-24 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Acrolepiopsis assectella ; lépidoptère ; phytophage spécialiste ; électroantennogrammes ; substances volatiles ; composés soufrés ; Allium ; plantes non-hôtes ; Acrolepiopsis assectella ; Lepidoptera ; phytophagous speciality ; electroantennograms ; volatiles ; sulphur compounds ; Allium ; non-host plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Summary Electroantennogram studies of adults Acrolepiopsis assectella Zell., Lepidoptera, Hyponomeutoidea, showed that the insects responded to the host-plant's odour whether or not the plant was healthy or damaged by the larvae. The antenna of both sexes also responded to the host-plant's specific volatile sulphur compounds. All these odours attracted the insects in the olfactometer. Propyl propane thiosulfinate (Ti-P2), the most abundant of the host specific compounds was always the most effective. The antenna was also sensitive to certain non-host plants (cabbage and tomato). The insect antenna was also sensitive to compounds of the ‘general green-leaf volatil complex’ which were identified in leek (Allium) odour. The most effective of these, cis-hexen-3-ol-1, was also the only attractive one. Sensitivity to these different olfactory stimuli was similar for 1-day-old females and 5-days-old males. The insect's sensitivity to green-leaf compounds increased with age for both sexes, only males responded better to Ti-P2 with age. These same males were also more attracted by leek odour at 5 days old. These facts confirm the predominant role of the sulphur compounds in host finding by the leek moth. The exact biological effect of green-leaf compounds and the interference exerted by non-host plants odours such as tomato on attraction to leek is discussed.
    Notizen: Résumé Les réponses E.A.G. d'Acrolepiopsis assectella Zell., Lépidoptère Hyponomeutoïdea spécialiste des Allium, montrent une sensibilité à l'odeur de la plante-hôte larvaire A. porrum saine ou attaquée, et à ses composés volatils soufrés spécifiques, toutes odeurs actives dans l'attraction des deux sexes en olfactomètre. Le Ti-P2, le plus abondant et le plus spécifique du végétal est toujours le plus efficace. Ceci confirme le rôle prépondérant des substances allélochimiques soufrées dans la recherche de la plante-hôte. Une réactivité antennaire plus ou moins importante est également enregistrée avec l'odeur de certaines plantes non-hôtes (chou et tomate). L'antenne du lépidoptère est également très sensible à certains produits volatils du ‘complexe odorant vert’ des végétaux qui sont identifiés dans l'odeur du poireau, le cis-hexen-3-ol-1 le plus actif est aussi le seul à être attractif. Le rôle biologique exact des ‘composés verts’ et l'interférence de l'odeur complète de la plantehôte avec celle de plantes non-hôtes, telle que la tomate, efficace sur l'antenne et sur le comportement sont discutés.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00187018
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    The reproductive capacity of Spodoptera littoralis moths fed ascorbic acid analogs (1986)
    Springer
    Entomologia experimentalis et applicata 41 (1986), S. 173-177 
    Details
    ISSN: 1570-7458
    Schlagwort(e): ascorbic acid analogs ; Spodoptera littoralis ; Lepidoptera ; moths reproduction ; spermatophore malformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Les examens ont porté sur l'action des acides D-ascorbique, L-déhydroascorbique, 6-chloro-6-déoxy-L-ascorbique, 6-bromo-6-déoxy-L-ascorbique et l'ascorbyl-2-sulfate per os. Les actions de ces analogues de l'acide ascorbique ont été comparées à celles de témoins alimentés avec vitamine C et glucose. L'acide D-ascorbique a provoqué la formation de spermatophores anormaux sans qu'il y ait eu réduction de l'activité sexuelle. Les femelles inséminées par ces mâles ont pondu des oeufs stériles, et leur fécondité était inférieure à celles de femelles alimentées sur des régimes contenant des analogues L de l'acide ascorbique ou du sucrose. L'acide 6-chloro-6-déoxy-L-ascorbique et l'ascorbyl-2-sulfate ont réduit la fécondité et la fertilité mais ces analogues n'avaient pas altéré la formation des spermatophores. Les consommations des différents analogues proposés séparément par microalimentation ou au moment de l'accouplement ne diffèrent pas significativement. La discussion a porté sur l'action des analogues dextrogyres sur le spermatophore et l'utilisation de ces analogues dans la protection des cultures.
    Notizen: Abstract D-ascorbic acid, L-dehydroascorbic acid, 6-chloro-6-deoxy-L-ascorbic acid, 6-bromo-6-deoxy-L-ascorbic acid and ascorbyl-2-sulfate were bioassayed per os on moths of Spodoptera littoralis (Boisd.). The activity of each of the analogs was compared with that of vitamin C and sucrose controls. D-ascorbic acid caused males to produce malformed spermatophores with no reduction in mating capacity. Females mated to these males layed infertile eggs and fecundity was lower than in females fed other analogs, L-ascorbic acid or sucrose diets. 6-chloro-6-deoxy-L-ascorbic acid reduced fertility and fecundity of the females, although this and other analogs did not affect the spermatophores. D-isoascorbic acid (=D-araboascorbic acid; D-erythorbic acid), D-glucoascorbic acid and all the other analogs were equally acquired by males microfed singly or at mating. The effect of D-optically active analogs on the spermatophore and the possible applications of the analogs in pest control are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00195574
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Population differences in larval hostplant use in the checkerspot butterfly, Euphydryas chalcedona (1986)
    Springer
    Entomologia experimentalis et applicata 40 (1986), S. 61-69 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Lepidoptera ; Nymphalidae ; Euphydryas chalcedona ; Scrophulariaceae ; Penstemon spp. ; hostplant specificity ; utilization efficiency ; iridoid glycoside ; insect/plant interactions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Des chenilles de deux populations d'E. chalcedona ont été élevées sur leur propre plante-hôte et sur celle de l'autre population, aux stades avant et après diapause. Les deux populations s'alimentent sur différentes espèces de Penstemon (Scrophulariaceae), et une population—Echo Lake—est monophage sur P. newberry, tandis que l'autre—Chico—utilise d'abord P. breviflorus, mais les chenilles après diapause sont trouvées sur au moins deux autres espèces de plantes. Les taux de croissance et de survie ont été déterminés pour des chenilles avant et après diapause pour les deux populations sur les deux plantes; les efficacités digestives ont été calculées sur les chenilles avant diapause. Les résultats ont montré que les chenilles des deux populations différaient par leur degré de spécialisation digestive sur leur plante hôte normale: les chenilles de Chico ont utilisé aussi bien les deux plantes, tandis que celles d'Echo Lake le faisaient significativement moins bien sur la plante non-hôte, par suite de l'inaptitude à la digérer. Ainsi la population oligophage est alimentairement moins spécialisée et plus capable de se débrouiller avec une plante non-hôte. Après diapause, les chenilles de Chico s'alimentaient significativement mieux sur plante hôte que non-hôte, ce qui était le cas aussi pour la population monophage. Dans l'ensemble, les chenilles de la population monophage semblaient moins capables de se débrouiller dans des conditions défavorables ou moins avantageuses.
    Notizen: Abstract Larvae from two populations of Euphydryas chalcedona Doubleday & Hewitson (Nymphalidae) were reared on their own hostplant and that of the other population, in both pre-diapause and post-diapause instars. One population, Chico, uses Penstemon breviflorus Lindl. (Scrophulariaceae), and the other, Echo Lake, uses P. newberryi Gray. Growth rate and survival were determined for pre-diapause and post-diapause larvae from both populations on both plant species; and digestive efficiencies were calculated during the prediapause instars. The results showed that larvae from the two populations differed in their responses to the two plant species. Pre-diapause larvae from Chico performed equally well on both plant species—survival and digestive indices were not significantly different for two Penstemon species. In contrast, pre-diapause larvae from Echo Lake performed significantly worse on the non-hostplant—growth and survival were significantly lower on the non-host, P. breviflorus. In addition, comparison of digestive efficiencies for the two plants showed that larvae from Echo Lake digested P. breviflorus better than P. newberryi, but were significantly less able to convert P. breviflorus to body mass. In the post-diapause instars, larvae from Chico grew faster on the host than on the non-host. Larvae from Echo Lake grew quite slowly on both plant species and significantly more of the Echo Lake larvae returned to diapause instead of completing development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00187024
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Perception of the oviposition deterring pheromone by tarsal and abdominal contact chemoreceptors in Pieris brassicae (1986)
    Springer
    Entomologia experimentalis et applicata 40 (1986), S. 71-79 
    Details
    ISSN: 1570-7458
    Schlagwort(e): oviposition deterrent ; contact chemoreceptors ; electrophysiology ; tarsi ; ovipositor ; Pieris brassicae ; Lepidoptera
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Une technique d'enregistrement apical a été utilisée pour examiner la perception d'une phéromone dissuadant la ponte par les poils récepteurs chimiques de contact des femelles de Pieris brassicae. Les réponses électrophysiologiques des poils gustatifs des tarses en présence de solutions de rinçage d'oeufs présentent une fréquence marquée des potentiels d'action provenant principalement d'une cellule sensorielle. Ceci suggère que les poils gustatifs des tarses des pattes antérieures des femelles possèdent, en plus de cellules répondant aux glucosinolates, des cellules sensorielles sensibles spécialement à la phéromone dissuadant la ponte. Des études morphologiques au microscope à balayage révèlent que l'oviposition de P. brassicae est pourvu de deux groupes de chimiorécepteurs de contact. Des enregistrements électrophysiologiques de ces sensilles révèlent la présence d'au moins trois cellules sensorielles, l'une d'entre elles étant un mécanorécepteur. La stimulation avec la solution de rinçage des oeufs évoque un léger accroissement de la fréquence des potentiels d'action qui ne peut être attribué à une cellule sensorielle particulière. Ceci indique que les poils gustatifs abdominaux peuvent participer d'une certaine façon à la perception de la phéromone dissuadant la ponte. Les réponses aux glucosinolates ne diffèrent pas significativement des stimulations témoin.
    Notizen: Abstract Perception of the oviposition deterring pheromone by contact chemoreceptors in female Pieris brassicae was studied employing a tip recording technique. Electrophysiological responses of tarsal taste hairs to eggwash solutions show a marked increase in frequency of spikes originating from only one sensory cell. This suggests that in foretarsal taste hairs females, apart from the glucosinolate cells also possess sense cells specifically sensitive to the oviposition deterring pheromone. Morphological studies by means of the scanning electron microscope revealed that the ovipositor of P. brassicae is provided with two groups of contact chemoreceptors. Electrophysiological recordings from these sensilla indicate the presence of at least three sensory cells, one of them being a mechanoreceptor. Stimulation with eggwash evokes a slight increase in spike frequency which cannot be ascribed to one particular sense cell. This indicates that abdominal taste hairs in some way may participate in the perception of the oviposition deterring pheromone. Responses to glucosinolates do not differ significantly from control stimulations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00187025
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    The effect of an oviposition deterring pheromone on egglaying in Pieris brassicae (1986)
    Springer
    Entomologia experimentalis et applicata 41 (1986), S. 139-146 
    Details
    ISSN: 1570-7458
    Schlagwort(e): oviposition deterrent ; pheromone ; egg washings ; Pieris brassicae ; Lepidoptera
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Des expériences de laboratoire ont été réalisées pour déterminer d'influence d'une phéromone dissuasive de la ponte (ODP) sur la distribution des ooplaques et des oeufs de Pieris brassicae. Cette phéromone est connue comme associée aux oeufs. Les papillons ont eu le choix entre des feuilles de chou traitées de différentes façons avec ODP et des feuilles témoins. La présence d'oeufs intacts de la même espèce sur les feuilles a un effet dissuasif modéré sur la ponte. Une solution aqueuse d'ODP, obtenue par lavage des oeufs à l'eau distillée est quelque peu dissuasive. Le lavage des oeufs de P. brassicae au méthanol est apparu comme le plus dissuasif. Une telle solution au méthanol peut être stockée à basse température au moins 3 ans sans perte de son pouvoir. Les applications de l'eau de rinçage sur les faces supérieures ou inférieures des feuilles induisent le même comportement des femelles. L'effet dissuasif augmente avec la concentration de l'eau de rinçage; aux très faibles concentrations, aucune différence significative n'a pu être observée avec les témoins quant au nombre d'ooplaques et d'oeufs. Par ailleurs, même aux très hautes concentrations du méthanol, l'eau de rinçage n'inhibe pas totalement la ponte sur les feuilles de chou. En lavant 7 fois consécutives les oeufs de P. brassicae dans le méthanol, on obtient une série de solutions de phéromones, dont chacune possède un fort pouvoir dissuasif. Bien que l'effet dissuasif diminue lentement avec des rinçages successifs, la pulvérisation du septième rinçage sur les feuilles de chou réduit encore à moins du quart le nombre total d'ooplaques et des oeufs pondus sur la feuille traitée.
    Notizen: Abstract A laboratory study was conducted in order to determine the influence of the oviposition deterring pheromone (ODP) upon the distribution of eggbatches and eggs by Pieris brassicae females. This pheromone is known to be associated with eggs. Butterflies were offered a choice between cabbage leaves treated in various ways with the ODP and control leaves. The presence of intact conspecific eggs on the treated leaf appeared to have a moderate deterrent effect upon oviposition. An aqueous solution of the ODP, obtained by washing eggs in distilled water was found to have a somewhat higher deterrent effect. Most effective in deterrence of oviposition, however, appeared to be a washing of P. brassicae eggs in methanol. Such a methanol solution can be stored at low temperatures for at least three years without loosing activity. Application of eggwash to either the upper or lower surface of the leaf does not make any difference to females. Percentage deterrence was found to increase with the concentration of eggwash. At very low concentrations no significant difference could be observed anymore in the numbers of eggbatches and eggs laid on control and treated leaf. On the other hand, even very high concentrations of methanol eggwash do not fully protect cabbage leaves against oviposition. Washing P. brassicae eggs seven times consecutively in methanol, a series of pheromone solutions is obtained, all of them were found to possess a high deterrent activity. Although percentage deterrence slowly decreases in subsequent washings, the seventh eggwash sprayed onto cabbage leaves still resulted in less than one quarter of the total number of eggbatches and eggs being laid on the treated leaf.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00195569
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Interacting effects of increased tannin levels on leaf-mining insects (1986)
    Springer
    Entomologia experimentalis et applicata 40 (1986), S. 297-301 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Lepidoptera ; Gracillariidae ; Cameraria sp. ; leaf miners ; mortality ; oaks ; parasitism ; tannins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Des extraits riches en tanins ont été étalés sur des feuilles de Quercus emoryi contenant des chenilles de ler stade de Cameraria sp., pour contrôler l'influence du tanin dans l'accroissement du parasitisme sans modifier la teneur en protéines ni provoquer de dommages physiques. Les mineuses de feuilles traitées avaient des taux plus élevés de parasitisme par les hyménoptères, mais une diminution des autres causes de mortalité. Les effets négatifs et positifs des tanins se sont équilibrés, puisque la survie des mineuses était la même pour les feuilles traitées que pour les témoins. Les extraits riches en tanin ont agi sur le développement larvaire puisque le poids des chrysalides des mineuses de feuilles traitées était plus faible. Les tanins peuvent ainsi avoir des effets nuisibles et bénéfiques sur ces insectes phytophages.
    Notizen: Abstract Tannin-rich extracts were painted on leaves of Q. emoryi (Fagaceae) that contained first instar larvae of a leaf miner, Cameraria sp. (Lepidoptera: Gracillariidae, Agrifoliella group), to test the role of tannins in increasing parasitism, while controlling for changes in protein content and physical damage. Miners from leaves that were treated with tannins had higher rates of parasitism by hymenopterans, but decreased mortality from other causes. The negative and positive effects of tannins balanced since overall larval survivorship was equivalent for leaf miners on tannin-enriched and control leaves. The tannin-rich extract also affected larval development because pupal weight was decreased for leaf miners in treated leaves. Tannins can have both adverse and beneficial effects on these phytophagous insects.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00293714
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Sex pheromone components ofMamestra suasa: chemical analysis, electrophysiological activity, wind tunnel activity and field tests in two European countries (1986)
    Springer
    Entomologia experimentalis et applicata 42 (1986), S. 291-299 
    Details
    ISSN: 1570-7458
    Schlagwort(e): (Z)-11-hexadecenyl acetate ; (Z)-11-hexadecenal ; (Z)-11-hexadecenol ; (Z)-9-tetradecenyl acetate ; hexadecanyl acetate ; Mamestra suasa ; Lepidoptera ; Noctuidae ; sex pheromone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé On a trouvé l'acetoxy-1 hexadécene-11 Z (Z-11-16:Ac), le hexadécene-11 Z al-1 (Z-11-16:Ald), le hexadécene-11 Z ol-1 (Z-11-16:OH) et l'acetoxy-1 hexadécene (16:Ac) dans des extraits de glandes phéromona les des femelles deMamestra suasa. La proportion relative des composés était 100/2/10/5. Tous les quatre composés ont été présents aussi dans les collections d'émanations des femelles en stade d'appel, dans la proportion un peu différente de 100/7/5/5. On n'a détecté aucune trace des tétradécenes al-1 ou d'acetoxy-1 tétradécenes. On a démontré la présence de hexadécenoate-1 methyl, hexadécene-9 Z oate-1 methyl et héxadécene-11 Z oate-1 methyl dans des extraits des glandes, par la méthode de ‘base methanolysis’. On n'a trouvé pas des tétradéceneoates methyl. En éléctroantennographie, Z-11-16:Ac a donné les meilleurs réponses, suivis par l'acetoxy-1 tétradécene-9 Z (Z-9-14:Ac), Z-11-16:Ald et Z-11-16:OH. Dans des études de ‘single sensillum’ les cellules à amplitude grande ont répondu à la stimulation avec de Z-11-16:Ac, cependant les cellules à amplitude petite ont répondu à la stimulation avec des deux composés Z-9-14:Ac et Z-11-16:OH. On a trouvé des cellules sensitives à Z-11-16:Ald dans 1 entre 60 sensilla étudiés. À la chambre de vol, le dose de 0.1 μg d'un mélange de 10:1 de Z-11-16:Ac/Z-11-16:Ald a provoqué les mêmes réponses et à l'intensité pareille comme 3 glandes phéromonales isolées des femelles. En piégeages sur le champs des males en quantité importante ont été capturé par un mélange de 10:1 de Z-11-16:Ac/Z-11-15:Ald en Bulgarie et Hongrie. L'addition de 16:Ac au mélange binaire n'avait aucun effet, cependant l'addition de plus de 1% de Z-11-16:OH ou 0.1% de Z-9-14:Ac a sérieusement diminué les captûres. En comparant des proportions différentes du mélange de l'acetoxy/aldéhyde dans des doses différentes, on a observé les meilleurs captûres avec de la proportion 10:1 et à la dose la plus haute (1 000 μg).
    Notizen: Abstract (Z)-11-hexadecenyl acetate (Z-11-16:Ac), (Z)-11-hexadecenal (Z-11-16:Ald), (Z)-11-hexadecenol (Z-11-16:OH) and hexadecanyl acetate (16:Ac) were found in pheromone gland extracts of femaleMamestra suasa (Den. et Schiff.) in the relative amounts 100/2/10/5. All four compounds were also present in collections of aiiborne volatiles from calling females in a 100/7/5/5 ratio. No traces of 14 carbon aldehydes or acetates were detected. In gland extracts the presence of methyl hexadecanoate methyl (Z)-9-hexadecenoate and methyl (Z)-11-hexadecenoate was demonstrated by base methanolysis. No methyl tetradecenoates were detected. In EAG tests Z-11-16:Ac gave the best responses, followed by (Z)-9-tetradecenyl acetate (Z-9-14:Ac) Z-11-16:Ald and Z-11-16:OH. In single sensillum recordings large spike amplitude cells in sensilla responded to Z-11-16:Ac, while small spike amplitude cells to both Z-11-16:OH and Z-9-14:Ac. Cells responding to Z-11-16:Ald were found in one out of 60 sensilla tested. In wind tunnel tests 0.1 μg of a 10:1 blend of Z-11-16:Ac/Z-11-16:Ald evoked the same responses and at a similar intensity as 3 isolated female pheromone glands did. In field tests a 10:1 blend of Z-11-16:Ac/Z-11-16:Ald caught significant numbers of males in both Bulgaria and Hungary. The addition of 16:Ac to the binary blend did not have any effect, while more than 1% of Z-11-16:OH or 0.1% of Z-9-14:Ac dramatically decreased captures. In comparing different ratios of the, acetate/aldehyde blend at different dose levels, best catches were recorded at the 10:1 ratio and at the highest (1000 μg) dose level.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00629317
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    The courtship behaviour of Yponomeuta padellus (1986)
    Springer
    Entomologia experimentalis et applicata 42 (1986), S. 45-55 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Yponomeuta padellus ; Lepidoptera ; Yponomeutidae ; courtship ; behaviour ; quantitative analysis ; pheromone communication ; wind tunnel
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Les séquences du comportement depuis l'attraction du mâle jusqu'à la copulation ont été décrites et analysées chez Y. padellus (L.). Les mâles réagissent à une femelle émenttant une phéromone par un hattement d'ailes suivi d'un vol en zigzag contre le vent. Les mâles atterriront près des femelles et marcheront avec leurs ailes en battement. Les antennes tambourinent le substrat et les pinceaux abdominaux sont déplyés. Lorsqu'il est au contact de la femelle, le mâle tambourine avec ses antennes les ailes, la tête et les antennes. Généralement suit une tentative d'étreinte. Les chances de succès sont les plus élevées quand les premiers contacts du mâle sont avec la partie postérieure de la femelle plutôt qu'avec la tête. Les femelles refusent les mâles en s'écartant ou en battant des ailes ou les deux à la fois. Il semble qu'en plus des stimuli cliniques, des stimuli mécaniques fournis par les écailles sont nécessaires pour que les mâles localisent la femelle et s'orientent pour une tentative d'étreinte.
    Notizen: Abstract The sequence of behavioural steps from male attraction until copulation is described and analyzed for Yponomeuta padellus L. (Yponomeutidae, Lepidoptera). Males respond to a pheromone emitting female with wing fanning followed by upwind zigzagging flight. Males will land near the females and then walk while wing fanning. The antennae drum onto the substratum and the abdominal-hair pencils are displayed. When in contact with a female the male drums with his antennae onto wings, head and antennae. Mostly a clasping attempt follows. A male has a higher chance to be accepted when he firt encounters the rear of a female instead of the head. Females refuse males by walking away or wing fanning or a combination of both.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00186157
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Influence du type de culture et de l'antécédent cultural sur les déplacements des adultes de la pyrale du maïs Ostrinia nubilalis (1986)
    Springer
    Entomologia experimentalis et applicata 41 (1986), S. 45-52 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Pyrale du maïs ; Ostrinia nubilalis ; Lepidoptera ; Pyralidae ; attraction plante-hôte ; peuplement-refuge ; piégeage lumineux ; lâcher insectes marqués ; coloration interne ; protandrie ; phéromone ; European corn borer ; Ostrinia nubilalis ; Lepidoptera ; Pyralidae ; host plant attraction ; refuge plant association ; light trapping ; internal coloration technique ; release of marked insects ; protandry ; pheromone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Summary The authors used their light trapping method associated with the release of marked insects. The experiment took place in an agrosystem in Charente (North of Aquitaine) between June 5 and July 15, 1983. The site included four adjoining plots from 1 to 3 ha each: one plot of wheat (previous crop: maize); two plots of maize (previous crop: barley) and a plot of barley (previous crop: wheat). To demonstrate the possible emergence of moths from hibernating larvae, the marked insects (510 ♂ ♂ and 467 ♀ ♀) were released in the plot of wheat equidistant from the four light traps placed in each plot. The captures of wild insects (1625 ♂ ♂, 786 ♀ ♀) showed an 8-days protandry and a considerable predominance of males in June/July 1983. This seems to have been caused by their being more mobile than the females. Despite the first males were distributed at random in the different plots, the remainder moved towards the maize. Such behaviour could be associated with that of the females; the latter move very early into the maize and probably lure the males by the release of sex-pheromone. The captures of marked insects (12% of the released population) confirmed the observations obtained with wild insects. The ecological and agronomical consequences of these migrations are discussed. It is suggested that there exists a pattern of two types of movements of European corn borer moths: emergence migration and diurnal migration.
    Notizen: Résumé Au cours de l'été 1983 dans un agrosystème de Charente (Nord du Bassin Aquitain), les auteurs ont utilisé la technique du piégeage lumineux associée à des lâchers d'insectes marqués. Afin de mimer les émergences possibles des papillons à partir de chenilles hivernantes, les insectes colorés étainent lâchés dans une parcelle de blé (Triticum) (à antécédent cultural maïs), à égale distance de 4 pièges disposés dans 4 parcelles adjacentes. Les captures d'insectes colorés montrent clairement que les femelles quittent le blé et se dirigent vers le maïs, confirmant par là le rôle de l'antécédent cultural sur les infestations annuelles. Les captures d'insectes sauvages indiquent à cette époque une prédominance des mâles due probablement à la protandrie d'environ 8 jours chez l'espèce et à une mobilité naturelle supérieure à celle des femelles. Les implications écologiques et agronomiques de ces déplacements sont discutées et un schéma d'explication des différents types de mouvements des papillons de pyrale: déplacements d'émergence et déplacements quotidiens alternatifs, est proposé.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00343488
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  • 10
    Digitale Medien
    Digitale Medien
    Synergism of positional isomers in sex-attractant systems of clearwing moths (Sesiidae) (1986)
    Springer
    Entomologia experimentalis et applicata 41 (1986), S. 311-313 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Lepidoptera ; Sesiidae ; clearwing moths ; Synanthedon ; sex attractants ; pheromone synergism ; pheromone receptors ; octadecadienyl acetates
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221262
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  • 11
    Digitale Medien
    Digitale Medien
    The disruption of proleg clasping by sublethal doses of chlordimeform in silk moth larvae: A possible mechanism of crop protection (1986)
    Springer
    Entomologia experimentalis et applicata 42 (1986), S. 9-17 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Lepidoptera ; behaviour ; formamidines ; pyrethroids ; prolegs ; crop protection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Des doses sublétales de chlordiméforme (CDM), insecticide formamidine, injectées dans des chenilles de Saturniidae, provoquent divers effets comportementaux. Ceux-ci comprennent l'arrêt de l'alimentation, l'augmentation du taux de locomotion, une hyperactivité générale de la tête et de ses appendices, et des mouvements désordonnés des pattes; Une conséquence de ce dernier effet est une réduction de l'étreinte réelle du substrat par les chenilles. Il a été étudié en détail sur les chenilles de cinquième stade de Philosamia cynthia ricini. Pour toute une gamme de doses, les effets les plus nets ont été observés sur les fausses pattes. Ils on été évalués par une série d'observations continues de chenilles placées sur une baquette de bois, permettant d'observer clairement le comportement d'étreinte. Les effet étaient réversibles. Des comparaisons ont été réalisées en utilisant la cyperméthrine, insecticide pyréthroïde. Les chenilles traitées ont présenté un certain nombre de comportements d'irritation, mais aucune réponse spécifique d'étreinte n'a été notée. La discussion de ces résultats examine les possibilités d'action de CDM dans la nature. Compte tenu de l'importance jouée par les fausses pattes dans le maintien sur la plante, on peut envisager que la suppression de l'étreinte peut entraîner une protection efficace des cultures.
    Notizen: Abstract General observations of the behavioural effects of sublethal doses of the fomamidine pesticide chlordimeform (CDM) on saturniid larvae led to a detailed investigation of the disruption of proleg clasping caused by CDM in Philosamia cynthia ricini Hutt. (Castor Silk Moth) larvae. Subjects received doses ranging from 5.09×10−3 to 19.3 μg/g body weight. Doses above 0.15 μg/g caused uncoordinated movements of the legs which led to reductions in the effective grip on the substrate. Greatest interference was recorded in the prolegs. The effects were reversible. Significance of these results is discussed in relation to their possible role in crop protection mechanisms. In investigating the degree of specificity of the observed effects as a mode of insecticidal action, larvae were treated with a pyrethroid compound, cypermethrin. Doses ranging from 0.002 to 0.842 μg/g resulted in reduction in the times the larvae spent firmly attached to the substrate. It is concluded that this is a consequence of an overall excitation caused by the insecticide, displayed as a number of described irritant behaviours, rather than any specific disruption of proleg action.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00186152
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  • 12
    Digitale Medien
    Digitale Medien
    Role of trichomes in soybean resistance to cabbage looper, Trichoplusia ni (1986)
    Springer
    Entomologia experimentalis et applicata 42 (1986), S. 109-117 
    Details
    ISSN: 1570-7458
    Schlagwort(e): cabbage looper ; Trichoplusia ni ; Glycine max ; soybeans ; trichomes ; plant resistance ; Noctuidae ; Lepidoptera
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé L'influence de la densité et de la longueur des trichomes de Glycine max sur la résistance à Trichoplusia ni a été évaluée suivant la position des feuilles sur une lignée résistante (‘PI 227687’) et sur un cultivar sensible (‘Davis’). Les feuilles apicales (juvéniles) tant de PI 227687 que de Davis, recouvertes de trichomes denses, résistèrent mieux à l'alimentation larvaire et à la survie de T. ni. Quand ces trichomes étaient éliminés, ces feuilles ne résistaient pas plus à l'alimentation des larves de T. ni que les feuilles non rasées TL3 et TL5 de PI 227687 ou toutes les autres feuilles de Davis. Les tests avec des extraits dans l'acétate d'éthyle et l'hexane de feuilles provenant de différentes positions de PI 227687 et Davis, destinés à mettre en évidence des phagodissuadants, ont montré que la résistance observée chez les feuilles apicales de Davis était attribuable aux trichomes (c'est-à-dire à un caractère morphologique), tandis que chez les mêmes feuilles de PI 227687 elle impliquait à la fois des trichomes (morphologie) et des substances chimiques, mais avec une prédominance de l'influence des trichomes.
    Notizen: Abstract Role of leaf trichome density and length in Glycine max (L.) Merr. resistance to Trichoplusia ni (Hübner) was evaluated at different leaf positions on a relatively resistant soybean, plant introduction (PI) 227687, and a relatively susceptible cultivar, ‘Davis’. The uppermost (juvenile) leaf within both PI 227687 and ‘Davis’ plants, which is densely covered with trichomes, was most resistant to T. ni larval feeding and survival. When these trichomes were shaven off, such leaves became as susceptible to T. ni larval feeding as unshaven TL3 and TL5 leaves (PI 227687) or all other unshaven leaves (‘Davis’). Bioassays for antifeedants in ethyl acetate and hexane extractables from leaves at the different positions on PI 227687 and ‘Davis’ plants showed that the resistance observed in the uppermost ‘Davis’ leaf is attributable to trichomes (i.e., a morphological factor); whereas, in the uppermost PI 227687 leaf it involves both morphological (trichomes) and chemical factors, but the trichome parameter is dominant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00343536
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  • 13
    Digitale Medien
    Digitale Medien
    Cellobiase activity in the larvae of Eri silk worm,Philosamia ricini Hutt. (1986)
    Springer
    Entomologia experimentalis et applicata 42 (1986), S. 301-302 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Lepidoptera ; Philosamia ricini ; Eri silk worm ; cellobiose ; cellobiase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00629318
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  • 14
    Digitale Medien
    Digitale Medien
    Polymorphisms in the genomic distribution of copia-like elements in related laboratory stocks ofDrosophila melanogaster (1986)
    Springer
    Journal of molecular evolution 24 (1986), S. 83-88 
    Details
    ISSN: 1432-1432
    Schlagwort(e): Transposons ; Polymorphism ; Drosophila ; Southern technique
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The genomic distributions of the copia, 297, 412, mdg 1, and B 104 transposable elements have been compared by the Southern technique among two Oregon R and four Canton SDrosophila laboratory lines that have been maintained separately for defined periods of a few years. The heterogeneity of the autoradiographic patterns suggests that multiple transposition events have occurred during the time of separation. The hypothesis that transposition could be induced by, variations of environmental parameters is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02099954
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  • 15
    Digitale Medien
    Digitale Medien
    Segmental organisation of the head in the embryo of Drosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 359-377 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Blastoderm fate map ; Head segmentation ; Larval cuticle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Embryos of Drosophila melanogaster were irradiated in the presumptive head region with a UV-laser microbeam of 20 μm diameter at two developmental stages, the cellular blastoderm and the extended germ band. The ensuing defects were scored in the cuticle pattern of the head of the first-instar larva, which is described in detail in this paper. The defects caused by irradiating germ band embryos when morphologically recognisable lobes appear in the head region were used to establish the segmental origin of various head structures. This information enabled us to translate the spatial distribution of blastoderm defects into a fate map of segment anlagen. The gnathal segments derive from a region of the blastoderm between 60% and 70% egg length (EL) dorsally and 60% and 80% ventrally. The area anterior to the mandibular anlage and posterior to the stomodaeum is occupied by the small anlagen of the intercalary and antennal segments ventrally and dorsally, respectively. The labrum, which originates from a paired anlage dorsally at 90% EL, is separated from the remaining head segments by an area for which we did not observe cuticle defects following blastoderm irradiation, presumably because those cells give rise to the brain. The dorsal and lateral parts of the cephalo-pharyngeal skeleton appear to be the only cuticle derivatives of the non-segmental acron. These structures derive from a dorso-lateral area just behind the putative brain anlage and may overlap the latter. In addition to the segment anlagen, the regions of the presumptive dorsal pouch, anterior lobe and post-oral epithelium, whose morphogenetic movements during head involution result in the characteristic acephalic appearance of the larva, have been projected onto the blastoderm fate map. The results suggest that initially the head of the Drosophila embryo does not differ substantially from the generalised insect head as judged by comparison of fate map and segmental organisation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00402870
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  • 16
    Digitale Medien
    Digitale Medien
    Lineage analysis of transplanted individual cells in embryos of Drosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 489-498 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Pole cells and midgut progenitors ; Cell lineages ; Embryogenesis ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In this paper experiments concerning some aspects of the development of pole cells and midgut progenitors in Drosophila are reported. Cells were labelled by injecting horseradish-peroxidase (HRP) in embryos before pole bud formation and transplanted at different stages into unlabelled embryos, where the transplanted cells developed together with the unlabelled cells of the host. The hosts were then fixed and stained at different ages in order to demonstrate the presence of HRP in the progenies of transplanted cells. The main conlusions of the study are as follows. The gonads are the only organ to the formation of which pole cells normally contribute; those pole cells which do not participate in the formation of the gonads are finally eliminated or degenerate. Since the number of primordial germ cells in the gonads is the same irrespective of the number of pole cells present in the embryo, an (unknown) mechanism must exist regulating the final number of pole cells in each of the gonads. After their formation and before reaching the gonads, pole cells have been found to divide only up to two times. With respect to the midgut progenitors, the cells of both anlagen have been found to be committed to develop into midgut, although they behave as equivalent in that they do not apparently distinguish between the anterior and posterior anlage. Midgut progenitors have been found to divide a maximum of three times and to produce two different types of cells, epithelial cells of the midgut wall and spindle-like cells located internally in the gut.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00375889
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  • 17
    Digitale Medien
    Digitale Medien
    Dominance for enzyme activity inDrosophila melanogaster (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 191-192 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Drosophila ; enzyme ; sn-glycerol-3-phosphate ; dehydrogenase ; dominance ; trans, regulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A regulatory element tightly linked to theGpdh locus inDrosophila melanogaster has been isolated from a natural population. Flies homozygous for second chromosomes bearing the element,H31, have half the GPDH activity of normal homozygotes. Heterozygotes betweenH31 andF orS alleles exhibit dominance in GPDH activity. Heterozygotes betweenH31, F orS andDf(2L) GdhA have half the diploid level. The contribution of theS allele to the activity inS/H31 heterozygotes is more than four times that ofH31. The regulatory element distinguishingH31 is tightly linked to theGpdh + locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01952463
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  • 18
    Digitale Medien
    Digitale Medien
    Acyl fluorides as reactive mimics of aldehyde pheromones: hyperactivation and aphrodisiac inHeliothis virescens (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 964-966 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Acid fluoride ; aldehyde ; mating disruption ; pheromone analogs ; Heliothis ; Lepidoptera ; fluorination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Substitution of fluorine for the aldehydic hydrogen provides behaviorally active, chemically reactive pheromone mimics. In male moths of the tobacco budwormHeliothis virescens, Z9–14:Acf and Z11–16:Acf cause hyperactivity and irreversible extension of the genitalia in over 80% of treated moths. In addition, a combination of the two components leads to 10–50% of the pairs involving one treated partner becoming locked in copula.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01941782
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  • 19
    Digitale Medien
    Digitale Medien
    Influence of host plant and larval diet on ovarian productivity inAcrolepiopsis assectella Z. (Lepidoptera: Acrolepiidae) (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 448-450 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Lepidoptera ; Acrolepiopsis assectella ; host plant ; larval diet ; ovarian production ; stimulation ; insemination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Females ofAcrolepiopsis assectella, reared on a semi-synthetic diet and laying on artificial substrates, do not respond to external stimuli by increasing ovarian production. When returned to the natural host (Allium porrum) for only one generation, ovarian production again rises and reaches the same level as in wild females, but its variability is strongly reduced. We conclude that selection under artificial conditions eliminates individuals which strictly depend on host plants for stimulation of larval nutrition and of reproduction.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02118653
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  • 20
    Digitale Medien
    Digitale Medien
    Pupation-temperature range in 12Drosophila species from different ecological backgrounds (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 600-604 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Drosophila ; temperature-effects ; pupation ; mating ; oviposition ; adaptive strategies
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A comparison of pupation-temperature range was made in the laboratory on a temperature gradient (3–38°C) using 12 species ofDrosophila representing four species groups and four different ecological backgrounds (temperate-montane forest:virilis group; desert;repleta group; cosmopolitan:melanogaster group; tropical forest:willistoni group). Within groups, differences are found which usually reflect species' distributions. Comparisons of species' mating-, oviposition- and pupation-temperature ranges reveal that pupation most-often occurs at temperatures beyond those for mating and oviposition. Each species reflects a different combination of temperature effects. Individual species have different temperature-limits for mating, oviposition and pupation. Temperatures permissive for one response are not predictive of limits on other responses. Among species, temperature can affect a particular response differently. Within groups, species differences can be at high and/or low temperatures for any response, and temperature effects among closely related species can manifest themselves in one, or any combination of responses. One cannot predict which responses will be most and least limited, or at which end of the temperature scale a response will be most limited. Among groups,common, but notabsolute temperature ranges generally correspond to the geographic distributions and ecological backgrounds of the species triads. The evaluation of temperature effects on species, based on a single activity, may not be adequate for predicting adaptive strategies.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01955553
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  • 21
    Digitale Medien
    Digitale Medien
    Clonal analysis of the blastoderm anlage of the Malpighian tubules in Drosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 22-32 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Cell lineage ; Malpighian tubules ; Compartments ; Cell death
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genetically marked maroon-like (mal) clones were induced by mitotic recombination with X-rays at the blastoderm stage in mal/mal + heterozygotes and were analysed in differentiated Malpighian tubules (MT). Marked cells were not confined to single anterior (MA) or posterior (MP) tubules, but were distributed among the four tubules. About 70% of the clones with two or more cells were fragmented, i.e. mal cells were separated by wild-type cells. Since the clones contain, on average, 6 cells and the differentiated MT consist of 484 cells (2 × 136 MA cells, 2 × 106 MP cells), we estimate that there are about 80 cells in the blastoderm anlage which on average pass through two to three mitoses. With increasing radiation doses (254 R, 635 R, 1270 R) a linear increase in clone frequency is observed. The mean sizes and size distributions of clones, however, remain unchanged. Since the increasing radiation dose also results in fewer differentiated Malpighi cells, we assume that regeneration does not occur. Therefore, size distributions of marked clones presumably represent real mitotic patterns in normogenesis. We suggest that essentially three successive mitoses take place, with a decreasing fraction of cells showing mitotic activity. Only a small fraction of cells goes through a fourth or even a fifth mitosis. Marked non-Minute clones induced in Minute heterozygotes are more frequent, but are not larger than non-Minute clones in wild-type background. Therefore, compartment boundaries cannot be recognized by this method. However, frequencies of marked cells found simultaneously in MA and MP pairs or in several single tubules of the same individuals are significantly higher than frequencies of multiple recombination events predicted by the Poisson distribution. From this, we conclude that neither the MA pair nor the MP pair nor single tubules represent compartments of the MT anlage.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00444038
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  • 22
    Digitale Medien
    Digitale Medien
    Lineage analysis of transplanted individual cells in embryos of Drosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 389-398 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Cell lineage ; Embryogenesis ; Drosophila ; Cell marking ; Cell transplantation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A method is presented which allows the study of the progeny of single cells during Drosophila embryogenesis. Cells from various larval anlagen of donor embryos labelled with a lineage tracer are individually transplanted from defined positions into similar, or different, positions in unlabelled hosts. The clones produced by these cells can be seen in whole mounts or in sections of fixed material, when using a histochemical marker (i.e. HRP), and/or in living embryos, when using fluorescent lineage tracers. The characteristics of the clones disclose lineage parameters, such as division patterns, morphogenetic movements and differentiation. The method is especially useful for testing the respective roles of positional information and cell lineage on the commitment of progenitor cells by transplanting these cells into heterotopic positions or into hosts of different genotypes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00402872
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  • 23
    Digitale Medien
    Digitale Medien
    Developmental genetics of a P element induced allele of suppressor-of-forked in Drosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 334-337 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Suppression ; P elements ; Lethality ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In this paper we describe a new allele of suppressor of forked, su(f) hd37, referred to as hd37, which was isolated in a hybrid dysgenesis mutation screen and is shown to be P induced by its high frequency of reversion in hybrid dysgenic crosses, and by in situ hybridization. hd37 suppresses forked and fails to complement the forked suppression of known su(f) alleles. However, it complements the recessive lethality of alleles in both of the su(f) lethal complementation groups. We also describe a new phenotypic effect of su(f) alleles, the enhancement of Minute(3)i 55. Recessive lethal alleles enhance the lethal effects of this Minute, but hd37 does not. The temperature sensitive period for forked bristle suppression by hd37 was found to be very narrow, consisting of a short interval (12–18 h) immediately before bristle formation. These results suggest that the several genetic functions associated with this locus may be genetically separable.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00376066
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  • 24
    Digitale Medien
    Digitale Medien
    The peripheral nervous system of mutants of early neurogenesis inDrosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 210-221 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Peripheral nervous system ; Neurogenesis ; Mutants ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Mutations previously known to affect early neurogenesis inDrosophila melanogaster have been found also to affect the development of the peripheral nervous system. Anti-HRP antibody staining has shown that larval epidermal sensilla of homozygous mutant embryos occur in increased numbers, which depend on the allele considered. This increase is apparently due to the development into sensory organs of cells which in the wild-type would have developed as non-sensory epidermis. Thus, neurogenic genes act whenever developing cells have to decide between neurogenic and epidermogenic fates, both in central and peripheral nervous systems. Different regions of the ectodermal germ layer are distinguished with respect to their neurogenic abilities.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02438953
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  • 25
    Digitale Medien
    Digitale Medien
    Maternal-effect mutations altering the anterior-posterior pattern of the Drosophila embryo (1986)
    Springer
    Development genes and evolution 195 (1986), S. 302-317 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Maternal effect Mutations ; Pattern formation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Mutations in seven different maternal-effect loci on the second chromosome of Drosophila melanogaster all cause alterations in the anterior-posterior pattern of the embryo. Mutations in torso (tor) and trunk (trk) delete the anterior- and posterior-most structures of the embryo. At the same time they shift cellular fates which are normally found in the subterminal regions of the embryo towards the poles. Mutations in vasa (vas), valois (vls), staufen (stau) and tudor (tud) cause two embryonic defects. For one they result in absence of polar plasm, polar granules and pole cells in all eggs produced by mutant females. Secondly, embryos developing inside such eggs show deletions of abdominal segments. In addition, embryos derived from staufen mothers lack anterior head structures, embryos derived from valois mothers frequently fail to cellularize properly. Mutations in exuperantia (exu) cause deletions of anterior head structures, similar to torso, trunk and staufen. However in exu, these head structures are replaced by an inverted posterior end which comprises posterior midgut, proctodeal region, and often malpighian tubules. The effects of all mutations can be traced back to the beginning stages of gastrulation, indicating that the alterations in cellular fates have probably taken place by that time. Analysis of embryos derived from double mutant mothers suggests that these three phenotypic groups of mutants interfere with three different, independent pathways. All three pathways seem to act additively on the system which specifies anterior-posterior cellular fates within the egg.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00376063
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  • 26
    Digitale Medien
    Digitale Medien
    Extra tarsal joints and abnormal cuticular polarities in various mutants ofDrosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 145-157 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Cell polarity ; Limb development ; Pattern formation ; Bristle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The legs of flies from 16 different mutant strains ofDrosophila melanogaster were examined for abnormal cuticular polarities and extra joints. The strains were chosen for study because they manifest abnormal cuticular polarities in some parts of the body (10 strains) or because they have missing or defective tarsal joints (6 strains). All but three of the stocks were found to exhibit misorientations of either the bristles, hairs, or “bract-socket vectors” on the legs. The latter term denotes an imaginary vector pointing from a hairlike structure called a “bract” to the bristle socket with which it is associated. On the legs of wild-type flies nearly all such vectors point distally, as do the bristles and hairs. In the mutant flies, the most common vector misorientation is a 180° reversal. When the bract-socket vectors of adjacent bristle sites in the same bristle row point toward one another, the distance between the sites is frequently abnormally large, whereas when the vectors point in opposite directions, the interval is frequently abnormally small. This correlation is interpreted to mean that bristle cells actively repel one another via cytoplasmic extensions that are longer in the direction of the bract-socket vector than in the opposite direction. Repulsive forces of this kind may be responsible for “fine-tuning” the regularity of bristle spacing in wild-type flies. Extra tarsal joints were found in eight of the 16 strains. A ninth strain completely lacking tarsal joints appears in some cases to have an extra tibia-basitarsus joint in its tibia. Whereas the tarsi of wild-type flies contain four joints, the tarsi ofspiny legs mutant flies contain as many as eight joints. In this extreme extra-joint phenotype, four of the joints correspond to the normal wild-type joints, and there is an extra joint in every tarsal segment except the distal-most (fifth) segment. Nearly all such ectopic extra joints have inverted polarity. In other strains the extra tarsal joints are located mainly at the wild-type joint sites, and joints of this sort have wild-type polarity. The alternation of normal and inverted (extra) joints inspiny legs resembles the alternation of normal and inverted (extra) body segment boundaries in the embryonic-lethal mutantpatch, suggesting that tarsal and body segmentation may share a common patterning mechanism.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02439432
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  • 27
    Digitale Medien
    Digitale Medien
    The sense organs in theDrosophila larva and their relation to the embryonic pattern of sensory neurons (1986)
    Springer
    Development genes and evolution 195 (1986), S. 222-228 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Sense organs ; Drosophila ; Pattern formation ; Peripheral nervous system
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Various types of sense organs are arranged in a highly reproducible pattern on the thoracic and abdominal segments ofDrosophila embryos and larvae. We describe this pattern and identify the neurons that innervate each sense organ. This identification is confirmed by the analysis of partial deficiencies for the scute region, which delete specifically some of the sense organs and their innervating neurons. Since our description of the sense organs accounts for all the sensory neurons that have been identified in the embryo, we believe that this description is accurate and complete, except in the terminal segment, where some sense organs remain to be identified.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02438954
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  • 28
    Digitale Medien
    Digitale Medien
    Lineage analysis of transplanted individual cells in embryos of Drosophila melanogaster (1986)
    Springer
    Development genes and evolution 195 (1986), S. 445-454 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Neural and epidermal cell lineages ; Embryogenesis ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Some aspects of neural and epidermal cell lineages during embryogenesis of Drosophila melanogaster were studied by transplanting horseradish-peroxidase-(HRP-) labelled ectodermal cells from young gastrula donors into host embryos of similar ages. Heterotopic transplantations permitted us to assess the degree of commitment already attained by the transplanted cells. The resulting cell clones showed normal characteristics of cytodifferentiation and cell number. The results indicate that epidermal progenitors perform a maximum of three mitoses during embryonic development, whereas neuroblasts may perform more than ten mitoses. Clone size distribution is in both cases scattered, suggesting either a rather irregular mitotic pattern or cell death. As indicated by heterotopic transplantations, the neurogenic ectoderm for the ventral nervous system exhibits different neurogenic abilities in its different regions, decreasing from medial to lateral; we discuss the hypothesis that some medially located cells of the young gastrulating embryo could be committed towards the neural fate before segregating from the ectoderm. On the other hand, the cells of the dorsal ectodermal regions at the same stage seem to be indifferent with respect to commitment, for they are able to give rise to central neural lineages following their transplantation in the neurogenic region.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00375748
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  • 29
    Digitale Medien
    Digitale Medien
    An interpretation of some bristle pattern modifications caused by directional selection inDrosophila melanogaster (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 846-848 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Drosophila ; bristles ; phenotype ; directional selection ; chaetogen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The variations of the dorsocentral and scutellar bristle patterns founded in two bidirectionaly selected lines are discussed in terms of the Richelle and Ghysen model. The phenotype obtained through selection for bristle suppression can be accounted for by a decrease in chaetogen production. Extra bristles can be accounted for by an alteration of the response of the cells to positional information.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01941549
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  • 30
    Digitale Medien
    Digitale Medien
    Confirmation of sex pheromone biosynthesis from (16-D3)palmitic acid in the turnip moth using capillary gas chromatography (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1059-1061 
    Details
    ISSN: 1420-9071
    Schlagwort(e): Pheromone ; Lepidoptera ; Agrotis segetum ; biosynthesis ; palmitic acid ; capillary gas chromatography ; deuterium ; polyethylene glycol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary (16-D3)palmitic acid was applied topically to pheromone glands of the turnip moth. After incubation omega labeled analogues of fatty acid methyl esters and acetates were identified by gas chromatography with flame ionization detection and selected ion monitoring. The pheromone components (Z)-5-decenyl, (Z)-7-dodecenyl- and (Z)-9-tetradecenyl acetate were all biosynthesized from palmitic acid along a common pathway.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01940732
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  • 31
    Digitale Medien
    Digitale Medien
    Immunochemical studies on xanthine dehydrogenase of soybean root nodules (1986)
    Springer
    Planta 167 (1986), S. 190-195 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Glycine (xanthine dehydrogenase) ; Immunocytochemistry ; Polyclonal antibody ; Root nodule ; Xanthine dehydrogenase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellels
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00391414
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  • 32
    Digitale Medien
    Digitale Medien
    Yeast communities from host plants and associated Drosophila in southern arizona: new isolations and analysis of the relative importance of hosts and vectors on comunity composition (1986)
    Springer
    Oecologia 70 (1986), S. 386-392 
    Details
    ISSN: 1432-1939
    Schlagwort(e): Yeast ; Drosophila ; Host plants ; Communities ; Vectors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The yeast communities from slime fluxes of three deciduous trees (Prosopis juliflora, Populus fremontii and Quercus emoryi) and the necroses of two cacti (Opuntia phaeacantha and Carnegiea gigantea) were surveyed in the region of Tucson, Arizona. In addition, the yeasts carried by dipterans associated with the fluxes or necroses (Drosophila carbonaria, D. brooksae, D. nigrospiracula, D. mettleri, and Aulacigaster leucopeza) were sampled. The results indicate that each host sampled had a distinct community of yeasts associated with it. The dipterans, which can act as vectors of the yeasts, deposited yeasts from other sources in addition to those found on their associated hosts. It is argued that host plant physiology is relatively more important than the activity of the vector in determining yeast community composition. Furthermore, the average number of yeast species per flux or necrosis is not different from the average number of yeast species per fly. It is hypothesized that the vector may affect the number of species per individual flux or not, and that the number is lower than the rot or necrosis could potentially support.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00379501
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  • 33
    Digitale Medien
    Digitale Medien
    The adaptive significance of alpine melanism in the butterfly Parnassius phoebus F. (Lepidoptera: Papilionidae) (1986)
    Springer
    Oecologia 70 (1986), S. 205-213 
    Details
    ISSN: 1432-1939
    Schlagwort(e): Parnassius ; Lepidoptera ; Radiation ; Temperature ; Melanism ; Thermoregulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The adaptive significance of alpine melanism, the tendancy for insects to become darker with increased elevation and latitude, was investigated using the butterfly Parnassius phoebus. The effects on temperature dependent activity of five components of overall wing melanism, as well as size, were examined. The components of wing melanism examined were the transparency of the basal hindwing and distal fore-wing areas, the width of the black patch in the basal hind-wing area and the proportion of black to white scales in that area, and the proportion of the distal fore-wing covered by predominantly black scaling. The body temperature of dead specimens was correlated with air temperature, solar radiation, the width of the black patch at the base of the wings, and the proportion of black to white scales at the base of the wings. The minimum air temperatures and solar radiation levels required for initiation of flight did not vary with wing melanism of P. phoebus, in contrast to the results found for Colias butterflies by Roland (1982). However, under environmental conditions suitable for flight initiation, males with a higher proportion of black to white scales in the basal area of the hind-wing, and wider basal black patches, spent a greater proportion of time in flight at low air temperatures and low insolation. Increased basal wing melanism was also associated with increased movement of males within a population. In contrast, melanism in the distal area of the wings has no effect on activities which are dependant on body temperature. The amount of time spent feeding did not vary with differences in wing melanism. I suggest that in dorsal basking, slow-flying butterflies (Parnassius) basal wing color affects body temperature primarily during flight (rather than while basking), such that butterflies with darker wing bases cool down less rapidly because they absorb more solar radiation during flight.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00379241
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  • 34
    Digitale Medien
    Digitale Medien
    Extracellular protein fibrils in Chrysochromulina breviturrita (Prymnesiophyceae) and their serological relationship to fungal fimbriae (1986)
    Springer
    Archives of microbiology 146 (1986), S. 207-213 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Extracellular proteins ; Surface fibrils ; Algae-fungi-Chrysochromulina ; Immunocytochemistry ; Agglutination ; Fimoriae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An extensive network of extracellular fibrils was revealed by negative staining in the greenish gold algal flagellate, Chrysochromulina breviturrita. These fibrils were of uniform diameter (4–5 nm), sometimes exceeding 5 μm in length. In addition there were short, narrower fibrils (2–3 nm) on the surface of the flagella. Six protein bands were isolated from spent culture medium by SDS-PAGE and one of 80,000 Da was found to polymerize after dialysis into 4–5 nm fibrils identical to those found on the cell surface. Two other proteins of 58,000 Da and 65,000 Da also formed 4–5 nm fibrils but these were either rare or of a shorter length and different appearance. An antiserum directed against the surface 7 nm fibrils (fimbriae) of fungi agglutinated cells of C. breviturrita and some other Prymnesiophyceae and Chrysophyceae, but did not agglutinate cells of algal species in other groups. Immunofluorescence and protein A gold labelling confirmed that antigens related to fungal fimbriae were present on the surface of cells of C. breviturrita. Only the 80,000 and 58,000 Da proteins labelled heavily following protein A gold labelling. Some individual 4–5 nm fibrils labelled with gold were observed in the material prepared from the 80,000 Da band. These results therefore establish that C. breviturrita produces a surface network of fibrils that are serologically related to the fimbriae of fungi, and suggest a previously unrecognized relationship between members of the Prymnesiophyceae, Chrysophyceae and fungal groups.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00403218
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  • 35
    Digitale Medien
    Digitale Medien
    Genetic and developmental characterization of the aldox-2 locus of Drosophila melanogaster (1986)
    Springer
    Biochemical genetics 24 (1986), S. 683-699 
    Details
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; aldox-2 ; molybdoenzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The aldox-2 locus in Drosophila melanogaster has been shown to affect differentially three molybdoenzymes, aldehyde oxidase, pyridoxal oxidase, and xanthine dehydrogenase. These effects are most obvious at times surrounding the pupal-adult boundary, when the normal organism accumulates large amounts of these enzymes in their active form. This locus has been more precisely mapped genetically to 2–82.9±2.1, with complete concordance between the effects of all recombinant chromosomes on all three enzymes. The cytogenetic location has also been determined to be between 52E and 54E8, with the likelihood that it lies within the region 54B1-54E8. The aldox-2 mutant allele has no visible phenotype and is completely recessive for enzyme effects at all stages tested. Segmental duplication of this region, including the aldox-2 + allele, has no apparent effect on the visible phenotype or the enzymatic activity. The mutant aldox-2 allele has no effect on the developmental expression of two unrelated enzymes, 6-phosphogluconate dehydrogenase and NADP+-dependent isocitrate dehydrogenase. The effects of this locus on aldehyde oxidase, xanthine dehydrogenase, and pyridoxal oxidase suggest that this locus may code for a product involved in the synthesis of the molybdenum cofactor common to these enzymes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00499002
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  • 36
    Digitale Medien
    Digitale Medien
    Analysis of Aldox n alleles isolated from natural populations of Drosophila melanogaster (1986)
    Springer
    Biochemical genetics 24 (1986), S. 291-308 
    Details
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; aldehyde oxidase ; gene dosage ; Aldox
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Aldox “null” alleles which were isolated from natural populations in Great Britain and North Carolina were analyzed for complementation. No complementation was observed between any combinations of “null” alleles for aldehyde oxidase (AO) specific activity in late third-instar larvae and newly emerged adults. AO immunologically cross-reacting material (AO-CRM) was quantitated in all homozygous stocks at both developmental stages as well as all allelic combinations in newly emerged adults. When the adult organism contains only Aldox n alleles, the polypeptides are not immunologically recognizable or may be rapidly degraded. Larvae and adults have different abilities to degrade mutationally altered enzymatically inactive AO polypeptide or synthesize them differentially. This is indicated by easily measurable AO-CRM levels in late third-instar larvae of Aldox n homozygotes, while newly emerged adult Aldox n homozygotes have very little, if any, AO-CRM. Newly emerged adult heterozygotes of Aldox n /Aldox + do have increased AO-CRM, indicating that the Aldox n alleles can code for a polypeptide which can be “rescued” if Aldox + gene product is present. Heterozygotes containing an Aldox + allele with a deficiency for the Aldox region produce 74.2% of the AO-CRM found in Aldox + homozygotes. This may indicate the presence of trans-acting factors which serve to activate gene expression in a system in which each gene copy is not maximally expressed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00502796
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  • 37
    Digitale Medien
    Digitale Medien
    The effect of temperature on biochemical and molecular properties ofDrosophila alcohol dehydrogenase (1986)
    Springer
    Biochemical genetics 24 (1986), S. 873-889 
    Details
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; alcohol dehydrogenase ; temperature ; adaptation ; enzyme polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The gene products of the two major alleles of alcohol dehydrogenase (ADH-F and ADH-S) have been subjected to kinetic and biochemical analyses over a range of temperatures. Although temperature was found to have a significant effect on both kinetic and biochemical properties ofDrosophila ADH, no significant differential effect was observed between the major ADH allozymes. The results are discussed within the context of the selective maintenance ofAdh polymorphism in natural populations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00554526
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  • 38
    Digitale Medien
    Digitale Medien
    sn-glycerol-3-phosphate oxidase and alcohol tolerance inDrosophila melanogaster larvae (1986)
    Springer
    Biochemical genetics 24 (1986), S. 859-872 
    Details
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; alcohol tolerance ; glycerol-3-phosphate oxidase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The role of sn-glycerol-3-phosphate oxidase (GPO; EC 1.1.99.5) in the variation of ethanol tolerance inDrosophila melanogaster was assessed in isofemale lines derived from individuals collected at the Chateau Tahbilk Winery and Wandin North Orchard of Victoria, Australia. When fed an undefined medium (semolina-treacle) with 6% ethanol (v/v), larvae of lines with high GPO activities survived better than did larvae of lines with low GPO activities. Although GPO was induced to higher activity levels by dietary ethanol in larvae of all the test lines, GPO activity was greater in lines representing the area outside the wine cellar. This implied that the cellar environment selected against individuals with high levels of GPO. These data do not explain the established difference in tolerance between cellar and outside populations. The GPO activities of lines were not dependent upon the activities of the lipogenic enzyme, glycerol-3-phosphate dehydrogenase; the major ethanol-degrading enzyme, alcohol dehydrogenase; or the citric acid cycle enzyme, fumarase. Thus, GPO activity is an important component of the metabolic mechanism of ethanol tolerance in larvae, but the mode of action of GPO has not been defined.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00554525
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  • 39
    Digitale Medien
    Digitale Medien
    Coadaptation ofDrosophila and yeasts in their natural habitat (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1037-1055 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Drosophila ; Diptera ; Drosophilidae ; yeasts ; cactus ; community ecology ; mutualism ; coadaptation ; evolution ; alkaloids ; fatty acids ; sterols
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The mutualistic interactions of cactophilicDrosophila and their associated yeasts in the Sonoran Desert are studied as a system which has evolved within the framework of their host cactus stem chemistry. Because theDrosophila-yeast system is saphrophytic, their responses are not thought to directly influence the evolution of the host. Host cactus stem chemistry appears to play an important role in determining where cactophilicDrosophila breed and feed. Several chemicals have been identified as being important. These include sterols and alkaloids of senita as well as fatty acids and sterol diols of agria and organpipe cactus. Cactus chemistry appears to have a limited role in directly determining the distribution of cactus-specific yeasts. Those effects which are known are due to unusual lipids of organpipe cactus and triterpene glycosides of agria and organpipe cactus.Drosophilayeast interactions are viewed as mutualistic and can take the form of (1) benefits to theDrosophila by either direct nutritional gains or by detoxification of harmful chemicals produced during decay of the host stem tissue and (2) benefits to the yeast in the form of increased likelihood of transmission to new habitats. Experiments on yeast-yeast interactions in decaying agria cactus provide evidence that the yeast community is coadapted. This coadaptation among yeasts occurs in two manners: (1) mutualistic increases in growth rates (which are independent of the presence ofDrosophila larvae) and (2) stabilizing competitive interactions when growth reaches carrying capacity. This latter form is dependent on larval activity and results in benefits to the larvae present. In this sense, the coadapted yeast community is probably also coadapted with respect to itsDrosophila vector.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01638995
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  • 40
    Digitale Medien
    Digitale Medien
    Synthesis of pungenin, a foliar constituent of some spruce species, and investigation of its efficacy as a feeding deterrent for spruce budworm [Choristoneura fumiferana (Clem.)] (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 251-260 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Picea pungens ; Picea glauca ; feeding deterrent ; spruce budworm ; Choristoneura fumiferana (Clem.) ; Lepidoptera ; Tortricidae ; pungenin ; protection of OH groups ; glucoside ; phenolic glucoside
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Pungenin was synthesized from 3,4-dihydroxyacetophenone by a short sequence involving manipulation of protecting groups on the 3 and 4 hydroxyl functions. Bioassays indicated that the glucoside is a modest feeding deterrent for sixth-instar spruce budworm larvae, but it does not appear to retard the development of small larvae or lead to increased mortality.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01045608
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  • 41
    Digitale Medien
    Digitale Medien
    Ultrastructure of exocrine prothoracic gland ofDatana ministra (Drury) (Lepidoptera: Notodontidae) and the nature of its secretion (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 2039-2050 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Datana ministra ; Lepidoptera ; Notodontidae ; exocrine prothoracic gland ; ultrastructure ; dispersal pheromone ; dodecanol ; dodecyl acetate ; dodecyl formate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Datana ministra larvae possess a gland situated in the ventral anterior portion of the prothorax. The gland consists of a single sac and is only charged with secretion in the last instar. Its orifice, a simple slit, opens into a transverse invagination of the integument in the cervical margin of the prosternite. The components of the gland have been identified by GC and GCMS and have been shown to be dodecanol, dodecyl acetate, and dodecyl formate. The morphology of the gland and the chemistry of the secretion are discussed in relation to other notodontid larvae which have been investigated. It is conjectured that the secretion is not defensive in nature but may function as a dispersal pheromone.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01041995
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  • 42
    Digitale Medien
    Digitale Medien
    Sex pheromone biosynthesis in the red-banded leafroller moth, studied by mass-labeling with stable isotopes and analysis with mass spectrometry (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 431-450 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Argyrotaenia velutinana ; Lepidoptera ; Tortricidae ; red-banded leafroller ; biosynthesis ; sex pheromone ; mass-labeling ; deuterium labeling ; carbon-13 labeling
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A technique for mass-labeling was developed to study sex pheromone biosynthesis in the red-banded leafroller moth,Argyrotaenia velutinana. With this technique, the pheromone components and all fatty acyl groups in the pheromone gland were analyzed for incorporation of label in the same analytic ran with gas chromatography-mass spectrometry, using chemical ionization and selected ion monitoring (GC-SIM-CI-MS). Sex pheromone glands were incubated with fatty acids or triacylglycerols labeled with at least three deuterium atoms or carbon-13 atoms. The results of these incubations support an interpretation in which hexadecanoate is chain shortened to tetradecanoate, which is desaturated to produce (E)- and (Z)-11-tetradecenoate precursors for the sex pheromone components (E)- and (Z)-11-tetradecen-1-yl acetate. Labeled (E)- and (Z)-11-tetradecenoyl groups in synthetic triacylglycerols were not incorporated into the sex pheromone components, perhaps indicating that this lipid class is not a donor of the immediate fatty acyl precursors in sex pheromone biosynthesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01020565
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  • 43
    Digitale Medien
    Digitale Medien
    Cardenolide connection between overwintering monarch butterflies from Mexico and their larval food plant,Asclepias syriaca (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1157-1170 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Danaus plexippus ; monarch butterflies ; Lepidoptera ; Danaidae ; Asclepiadaceae ; milkweeds ; Asclepias syriaca ; Asclepias speciosa ; ecological chemistry ; chemical defense ; cardenolides ; aspecioside ; desglucosyrioside ; syriobioside ; overwintering
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The majority (85%) of 394 monarch butterflies sampled from overwintering sites in Mexico contain the same epoxy cardenolide glycosides, including most conspicuously a novel polar glycoside with a single genin-sugar bridge (aspecioside), as occur in the milkweedsAsclepias speciosa andA. syriaca. This cardenolide commonality was established by isolating aspecioside and syriobioside from the wings of overwintering monarchs and the two plant species, and comparing Chromatographie and NMR spectrometric characteristics of the isolates. When combined with the migratory pattern of monarchs and the distribution of these two milkweed species, this chemical evidence lends strong support to the hypothesis thatA. syriaca is the major late summer food plant of monarchs in eastern North America. This finding may be of ecological importance, forA. syriaca contributes less cardenolide and cardenolides of lower emetic potency to monarchs than most milkweeds studied to date.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01639002
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  • 44
    Digitale Medien
    Digitale Medien
    Studies on identification and syntheses of insect pheromones XXII (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1263-1271 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Paranthrene tabaniformis ; poplar twig clearwing moth ; sex pheromone ; Lepidoptera ; Aegeriidae ; (E,Z)-3,13-octadecadien-1-ol
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract (E,Z)-3,13-Octadecadien-1-ol (la) was identified as the sex pheromone from the poplar twig clearwing moth,Paranthrene tabaniformis, females by gas chromatography-mass spectrometry analysis, synthesis, and laboratory bioassays. In the field tests, a trap baited with 200 μg synthetic la caught more male moths than two live female moths.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01012346
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  • 45
    Digitale Medien
    Digitale Medien
    Stereoselective synthesis of (Z,E)-3,5-tetradecadienyl acetate: Sex attractant for carpenterworm moth,Prionoxystus robiniae (Peck) (Lepidoptera: Cossidae) and effect of isomers and monounsaturated acetates on its attractiveness (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 619-633 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Prionoxystus robiniae ; Lepidoptera ; Cossidae ; Carpenterworm ; sex attractant ; (Z,E)-3,5-tetradecadienyl acetate ; inhibition ; synergism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract A new efficient stereoselective synthesis for (Z,E)-3,5-tetradecadienyl acetate (Z3,E5–14∶Ac) a potent male sex attractant for the carpenterworm,Prionoxystus robiniae (Peck), was developed. The effects of the other three isomers (Z,Z; E,Z; E,E) on the field attractiveness of theZ,E isomer toward maleP. robiniae were determined. TheZ, Z isomer inhibited attraction, theE, E isomer synergized attraction, and theE,Z isomer had no effect on attraction. Seven monounsaturated 14-carbon acetates were evaluated for their effect on the attractiveness ofZ3,E5–14∶Ac. (E)-3-Tetradecenyl acetate enhanced attraction and (Z)-9- and (E)-5-tetradecenyl acetate reduced trap captures. (Z,E)-3,5-Tetradecadien-1-ol also reduced the attractiveness ofZ3,E5–14∶Ac.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01012097
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  • 46
    Digitale Medien
    Digitale Medien
    Euphydryas anicia (Lepidoptera: Nymphalidae) utilization of iridoid glycosides fromCastilleja andBesseya (Scrophulariaceae) host plants (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1459-1468 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Euphydryas anicia ; Lepidoptera ; Nymphalidae ; Castilleja integra ; Besseya plantaginea ; Besseya alpina ; iridoid glycosides ; catalpol ; macfadienoside ; sequestration ; plant-insect interactions ; herbivory ; ecological chemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Iridoid glycosides were found to be sequestered by natural populations ofEuphydryas anicia after ingestion from the host plantsBesseya alpina, B. plantaginea, andCastilleja integra. Both major iridoids ofB. alpina, cataipol and aucubin, were found in butterfly populations where this was the only host plant. The catalpol-aucubin ratio was higher in the butterflies than in the host plant. AnE. anicia population which uses bothB. plantaginea andC. integra as host plants was found to sequester cataipol as well as another iridoid, macfadienoside. Macfadienoside was the major iridoid ofC. integra, while catalpol esters were the major iridoids ofB. plantaginea. Although it was a major sequestered iridoid, catalpol was a minor constituent in both host plants. The macfadienoside-catalpol ratio in the butterflies from this population was highly variable, and there appeared to be both sex and individual variation in host plant and/or iridoid glucoside utilization byE. anicia. Although other iridoids were present in the host plants, none was sequestered in more than trace amounts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01012364
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  • 47
    Digitale Medien
    Digitale Medien
    Behavioral and growth responses of specialist herbivore,Homoeosoma electellum, to major terpenoid of its host,Helianthus SPP (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1505-1521 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Chemical defense ; feeding behavior ; glandular trichomes ; Helianthus ; herbivory ; Homoeosoma electellum ; Lepidoptera ; Pyralidae ; host-plant resistance ; plant-insect interactions ; sesquiterpene lactones ; terpenoids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The responses of a sunflower specialist,Homoeosoma electellum, the sunflower moth, to the terpenoids produced by its host plant,Helianthus, were measured. Larvae were reared on synthetic diet containing one of three concentrations of the dominant sesquiterpene lactone found in glandular trichomes ofH. maximilliani. Treatments were initiated at each of three larval ages. Pupal weight was significantly reduced, but the effect diminished as the larvae aged. Survival and development time were unaffected by various treatments. In behavioral tests, larvae showed no preference for untreated synthetic diet compared to diet containing the secondary compound at a concentration of 1% by dry weight. When the concentration was raised to 5%, all but last-instar larvae showed a significant preference for the untreated diet. A second behavioral test measured the damage done to whole florets and an array of floral parts offered to larvae of different ages. The damage pattern of floral tissues changed as the larvae grew older, demonstrating that the willingness of larvae to eat tissues contaminated with trichome contents increased with age. The value of the glandular trichome contents as a defense againstH. electellum is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01012368
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  • 48
    Digitale Medien
    Digitale Medien
    Female sex pheromone of the melonworm,Diaphania hyalinata (Lepidoptera: Pyralidae), and analysis of male responses to pheromone in a flight tunnel (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 229-237 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Diaphania hyalinata ; D. nitidalis ; melonworm ; pickleworm ; Lepidoptera ; Pyralidae ; sex pheromone ; insect behavior ; flight tunnel ; 10,12-hexadecadienal ; 11-hexadecenal
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Ten C16 chain-length compounds were identified from heptane extracts of ovipositors of female melonworm,Diaphania hyalinata (L.). The major constituents of the extracts were (E)-11-hexadecenal and (E,E)-10,12-hexadecadienal [(E,E)-10,12–16:Ald] and the alcohols and acetates of these olefins were found in trace amounts (〈2%). Extracts also contained traces of (E,Z)- and (Z,Z)-10,12-16:Ald, hexadecanal, and 1-hexadecanol. Analysis of the behavioral responses of males to synthetic mixtures of these compounds and responses to ovipositor extracts in a flight tunnel showed that a synthetic mixture of the 10 compounds elicited a behavioral repertoire from males that was indistinguishable from that elicited by ovipositor extract. Flight tunnel studies also indicated that six of the 10 compounds probably represent the essential components of the female's sex pheromone.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01045606
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  • 49
    Digitale Medien
    Digitale Medien
    Dietary effects of phytoecdysones in the leek-moth,Acrolepiopsis assectella Zell. (Lepidoptera: Acrolepiidae) (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 1979-1986 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Allium porrum L. ; Liliaceae ; Acrolepiopsis assectella Zell. ; leek-moth ; Lepidoptera ; Acrolepiidae ; ecdysone ; ecdysteroids ; 20-hydroxyecdysone ; 5,20-dihydroxyecdysone ; 20-hydroxy ; 25-deoxyecdysone ; ecdysial failures
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Incorporation of certain phytoecdysones (ecdysterone, polypodine B, and ponasterone A) into a semisynthetic artificial diet induces pathophysiological effects in larvae of the leek-moth (Acrolepiopsis assectella Zell., Acrolepiidae). The effects include lethality of the newly hatched, first-instar larvae; special ecdysial failures associated with the appearance of larvae with two head capsules; and developmental anomalies during metamorphosis. The effective range of dietary ecdysteroid, as evaluated by larval mortality, varies from 25 to 250 ppm. The EC50 value is 100 ppm for polypodine B and 130 ppm for ecdysterone. The dietary effects of the phytoecdysones are similar to the previously observed effects caused by the dried flowers, but not leaves, of the leek plant. However, the active compound of the leek flowers is a saponin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01041947
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  • 50
    Digitale Medien
    Digitale Medien
    Pheromone biosynthesis and role of functional groups in pheromone specificity (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 335-351 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Acetate esters ; alcohols ; aldehydes ; fatty acid biosynthesis ; insect pheromones ; Lepidoptera ; pheromone biosynthesis ; spruce budworm
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The sex attractants for many Lepidoptera are long-chain, monounsaturated acetate esters, alcohols, and aldehydes. In vivo metabolic studies and enzyme analysis in vitro have provided evidence that the aldehyde pheromone of the eastern spruce budworm is synthesized de novo via an acetate ester precursor. Interconversion of the functional groups (ester, alcohol, aldehyde) can explain differences in the pheromone blends used by closely related species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01020560
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  • 51
    Digitale Medien
    Digitale Medien
    Habitat choice byDrosophila pseudoobscura: The roles of genotype and of experience (1986)
    Springer
    Behavior genetics 16 (1986), S. 271-279 
    Details
    ISSN: 1573-3297
    Schlagwort(e): Drosophila ; habitat choice ; learning ; experience
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract Microhabitat preferences ofDrosophila pseudoobscura strains were examined in a Waddington maze, with an emphasis on learning how early environment affected adult habitat choice. The genotypes were roughly those expected in a natural population; the environmental variables included light, temperature, and food. It was found that (1) the different genotypes chose habitats differently; (2) early experience affected subsequent habitat choice; and (3) the effect of early experience was complex, as preference for one niche dimension (temperature) was reinforced by experience with the generally preferred value, preference for another niche dimension (light) was weakened by experience with the generally preferred value, and preference for other niche dimensions (food) was generally unaffected by experience. In this study the contribution to the total chi square was about equal from genotype and from environment. The significance of these findings for studies of dispersal and population structure of natural populations is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01070802
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  • 52
    Digitale Medien
    Digitale Medien
    Measuring learning in individual flies is not necessary to study the effects of single-gene mutations inDrosophila: A reply to Holliday and Hirsch (1986)
    Springer
    Behavior genetics 16 (1986), S. 449-455 
    Details
    ISSN: 1573-3297
    Schlagwort(e): Drosophila ; learning ; memory ; classical conditioning ; mutants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract Holliday & Hirsch (this issue) now agree that “Quinnet al. (1974) have demonstrated learning [inDrosophila] with group data, and their inability to identify individual differences (IDs) in performance does not invalidate their conclusion that some individuals in the population must have learned.” However, they consider it important, if not necessary, to show that anindividual fly has learned. In response to Holliday and Hirsch, this paper discusses why it is not necessary to measure learning in individual fruit flies before searching for underlying biochemical mechanisms.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01074264
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  • 53
    Digitale Medien
    Digitale Medien
    The effect of dietary ethanol on the composition of lipids of Drosophila melanogaster larvae (1986)
    Springer
    Biochemical genetics 24 (1986), S. 51-69 
    Details
    ISSN: 1573-4927
    Schlagwort(e): ethanol ; lipid ; alcohol dehydrogenase ; Drosophila ; nutrition
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract At a moderate concentration (2.5%, v/v) dietary ethanol reduced the chain length of total fatty acids (FA) and increased the desaturation of short-chain FA in Drosophila melanogaster larvae with a functional alcohol dehydrogenase (ADH). The changes in length in total FA were postulated to be due to the modulation of the termination specificity of fatty acid synthetase. Because the ethanol-stimulated reduction in the length of unsaturated FA was blocked by linoleic acid, it was thought to reflect the properties of FA 9-desaturase. Although the ethanol-stimulated reduction in chain length of unsaturated FA was also observed in ADH-null larvae, ethanol promoted an increase in the length of total FA of the mutant larvae. Thus, the ethanolstimulated change in FA length was ADH dependent but the ethanol effect on FA desaturation was not. Ethanol also stimulated a decrease in the relative amount of phosphatidylcholine and an increase in phosphatidylethanolamine. Because similar ethanol-induced changes have been found in membrane lipids of other animals, ethanol may alter the properties of membranes in larvae. It is proposed that ethanol tolerance in D. melanogaster may be dependent on genes that specify lipids that are resistant to the detrimental effects of ethanol.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00502978
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  • 54
    Digitale Medien
    Digitale Medien
    Relationship between α-glycerophosphate dehydrogenase activity and metabolic rate during flight in Drosophila melanogaster (1986)
    Springer
    Biochemical genetics 24 (1986), S. 245-257 
    Details
    ISSN: 1573-4927
    Schlagwort(e): flight metabolism ; Drosophila ; αGPDH ; Kacser-Burns
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Measurements of wing-beat frequency (WBF) have been used to characterize flight muscle metabolic rate in Drosophila melanogaster during tethered flight. Progeny of crosses between 17 X-chromosome substitution lines and three null-activity stocks have been studied in order to determine the effect on flight metabolism of sharply reduced activity of α-glycerophosphate dehydrogenase (αGPDH). It was found that flies with an approximate 50% reduction in αGPDH activity have a metabolic rate that is, in most cases, indistinguishable from that of wild-type flies and, in the most extreme cases, reduced by only 4%. These results demonstrate that αGpdh is not a “major gene” for flight metabolism, in the quantitative genetic sense of the term. These results are in agreement with the Kacser and Burns (1973, 1979, 1981) theory of flux, which postulates that the activity of an enzyme embedded in a multienzyme pathway can sometimes vary from wild-type to very low levels (perhaps 5–10% wild type) with no significant effect on flux through the total pathway.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00502792
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  • 55
    Digitale Medien
    Digitale Medien
    Neuroendocrine cells within colorectal tumours induced by dimethylhydrazine (1986)
    Springer
    Cell & tissue research 246 (1986), S. 205-210 
    Details
    ISSN: 1432-0878
    Schlagwort(e): 1,2-Dimethylhydrazine ; Peptide YY ; Glucagon ; 5-Hydroxytryptamine ; Immunocytochemistry ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Colorectal adenocarcinomas were induced in male Wistar rats, by weekly subcutaneous administration of 1,2-dimethylhydrazine, classified according to the degree of differentiation and submitted to immunocytochemistry for the peptides cholecystokinin (CCK), gastrin, gastric inhibitory polypeptide (GIP), glucagon, neurotensin, pancreatic polypeptide (PP), peptide YY (PYY), somatostatin and vasoactive intestinal polypeptide (VIP) and the biogenic monoamine 5-hydroxytryptamine. Well- or moderately well-differentiated adenocarcinomas comprised 46% of the tumour population, only 4% were poorly-differentiated adenocarcinomas, and the remaining 50% possessed a mixture of these two morphologies. Glucagon, PYY and 5-hydroxytryptamine immunoreactive cells were frequently observed within well- or moderately well-differentiated tumours and within such regions of tumours possessing a mixed morphological pattern. The tumours contained no cells immunoreactive for any of the peptides not normally located within the colorectum, nor did they contain cells immunoreactive for somatostatin and VIP, although known positive controls did stain. Poorly-differentiated tumours and portions of tumours of mixed type, were consistently negative. 5-hydroxytryptamine was the most frequently located of the three antigens, being detected in 87% of the moderately well-differentiated tumours and 32% of the tumours with mixed morphologies. 11% of moderately well-differentiated tumours possessed 5-hydroxytryptamine positive cells in such profusion that they contributed significantly to the tumour mass. The distribution of glucagon-and PYY-immunoreactive cells was similar, although they occurred with a lower frequency, presumably corresponding to their lower numbers within the normal colorectal mucosa. Additionally, these two peptide immunoreactivities were colocalized in the majority of cells, although some cells contained only one antigen. The immense numbers of cells immunoreactive for peptides and monoamine in a significant proportion of colorectal adenocarcinomas suggests that they have arisen from multipotential endodermal stem cells within the tumours and are not part of the normal epithelial population being engulfed as the tumour grows.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219019
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  • 56
    Digitale Medien
    Digitale Medien
    Immunocytochemistry of magnocellular neurons of supraoptic and paraventricular nuclei of normal and Brattleboro rats with vasopressin anti-idiotype antibody (1986)
    Springer
    Cell & tissue research 246 (1986), S. 509-513 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Anti-idiotype antibody ; Vasopressin ; Immunocytochemistry ; Receptors ; Brattleboro rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A vasopressin anti-idiotype antibody was generated by immunization with purified IgG of a primary vasopressin antiserum. The anti-idiotype antibody immunostained neurons in the supraoptic and paraventricular nuclei of the hypothalamus of normal and Brattleboro rats. The distribution of immunostained perikarya in these hypothalamic nuclei together with the staining of fibers in median eminence and neural lobe was similar to that observed in normal rats with anti-vasopressin and suggests strongly that vasopressinergic neurons are being stained. Absorption studies with vasopressin and a vasopressin-binding receptor protein further indicate that a receptor associated with vasopressinergic neurons is recognized by the anti-idiotype antibody.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215190
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  • 57
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of a rhodopsin-like protein in the lipochondria in photosensitive neurons of Aplysia californica (1986)
    Springer
    Cell & tissue research 244 (1986), S. 115-120 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neurons ; Lipochondria ; Rhodopsin ; Immunocytochemistry ; Aplysia californica
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Polyclonal antibodies directed against squid opsin were used in immunocytochemical and immunoblot experiments to identify a rhodopsin-like protein in photosensitive neurons of Aplysia. Aldehyde-fixed abdominal and cerebral ganglia were embedded in paraffin for peroxidase anti-peroxidase analysis or used whole for immunofluorescence studies. Ganglia were embedded in Lowicryl K4M for electron-microscope immunocytochemistry. In both the cerebral and abdominal ganglia, light-microscope immunocytochemical results showed reaction product deposited around the neuronal cell periphery corresponding in position to the lipochondria. In the abdominal ganglion, the giant cell R2, located in the right rostral quarter, and neurons in the right caudal quarter were consistently labeled with anti-opsin. Electron-microscopic studies demonstrated ferritin-labeling of the lipochondria in R2 and other immunoreactive neurons. Immunoblot analysis of R2 and cerebral neuron extracts was used to identify two prominent immunoreactive protein bands at 85000 and 67500 molecular weight.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218388
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  • 58
    Digitale Medien
    Digitale Medien
    GABA neurones in retinas of different species and their postnatal development in situ and in culture in the rabbit retina (1986)
    Springer
    Cell & tissue research 243 (1986), S. 117-123 
    Details
    ISSN: 1432-0878
    Schlagwort(e): GABA ; Immunocytochemistry ; Neurones ; Retina ; Different species ; Rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The localisation of GABA immunoreactive neurones in retinas of a variety of animals was examined. Immunoreactivity was associated with specific populations of amacrine neurones in all species examined, viz. rat, rabbit, goldfish, frog, pigeon and guinea-pig. All species, with the exception of the frog, possessed immunoreactive perikarya in their retinal ganglion cell layers. These perikarya are probably displaced amacrine cells because GABA immunoreactivity was absent from the optic nerves and destruction of the rat optic nerve did not result in degeneration of these cells. GABA immunoreactivity was also associated with the outer plexiform layers of all the retinas studied; these processes are derived from GABA-positive horizontal cells in rat, rabbit, frog, pigeon and goldfish retinas, from bipolar-like cells in the frog, and probably from interplexiform cells in the guinea-pig retina. The development of GABA-positive neurones in the rabbit retina was also analysed. Immunoreactivity was clearly associated with subpopulations of amacrine and horizontal cells on the second postnatal day. The immunoreactivity at this stage is strong, and fairly well developed processes are apparent. The intensity of the immunoreactivity increases with development in the case of the amacrine cells. The immunoreactive neurones appear fully developed at about the 8th postnatal day, although the immunoreactivity in the inner plexiform layer becomes more dispersed as development proceeds. The immunoreactive horizontal cells become less apparent as development proceeds, but they can still be seen in the adult retina. The GABA immunoreactive cells in rabbit retinas can be maintained in culture. Cultures of retinal cells derived from 2-day-old animals can be maintained for up to 20 days and show the presence of GABA-positive cells at all stages. In one-day-old cultures the GABA immunoreactive cells lacked processes but within three days had clearly defined processes. After maintenance for 10 days a meshwork of GABA-positive fibres could also be seen in the cultures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221859
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  • 59
    Digitale Medien
    Digitale Medien
    Morphological variation of immunoreactive cells positive to cholecystokinin 33 (10–20) and gastrin 34 (1–15) in human duodenum (1986)
    Springer
    Cell & tissue research 244 (1986), S. 519-525 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Duodenum ; Cholecystokinin ; Gastrin ; Immunocytochemistry ; Ultrastructure ; Man
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Human duodenal endocrine cells reactive with antibodies to cholecystokinin (CCK) 33 (10–20) and/or gastrin 34 (1–15) were studied by a combination of immunohistochemical and electron-microscopic methods. By immunohistochemistry, three types of endocrine cells were distinguished in human duodenal mucosa, i.e., those only positive for only CCK, those positive for both CCK and gastrin and those only positive for only gastrin. Ultrastructurally, the first cell type is characterized by many secretory granules with an eccentric dense core (mean diameter; 271+-74 nm). The second cell type, which was less frequent than the other two, has ultrastructural features that resemble type-I cells. The last cell type was composed of two types of cells containing small secretory granules identical to those of IG cells (mean diameter; 171+-31 nm) or large secretory granules indistinguishable from those of I cells (mean diameter; 286+-50 nm).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00212529
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  • 60
    Digitale Medien
    Digitale Medien
    The dopaminergic innervation of the goldfish pituitary (1986)
    Springer
    Cell & tissue research 244 (1986), S. 577-582 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Dopamine ; Pituitary gland ; Neuroendocrine regulation ; Immunocytochemistry ; Carassius auratus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The dopaminergic innervation of the goldfish pituitary gland was studied by immunocytochemistry at the electron-microscope level using highly specific antibodies against dopamine coupled to bovine serum albumin with glutaraldehyde. A satisfactory preservation of the tissue was achieved after immersion in 5% glutaraldehyde in phosphate buffer containing sodium metabisulfite to prevent oxidation of the endogenous dopamine. The immunocyto-chemical procedure was performed on Vibratome sections using the preembedding method. Immunoreactivity was restricted to part of the neurosecretory type-B fibers (diameter of the secretory vesicles lower than 100 nm) in which it was found to occupy the whole cytoplasm. Labeled fibers were observed within the neurohypophysis in the different parts of the gland and in the adenohypophyseal tissue where immunoreactive profiles were detected in close apposition to the different cell types. These data are in agreement with previous results obtained by means of radioautography and further support a role for dopamine in the neuroendocrine regulation of pituitary functions in teleosts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00212536
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  • 61
    Digitale Medien
    Digitale Medien
    Glutamic acid decarboxylase (GAD)-like immunoreactivity in the pedal ganglion of Mytilus galloprovincialis (1986)
    Springer
    Cell & tissue research 244 (1986), S. 591-593 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; GAD ; Pedal ganglion ; Invertebrate nervous system ; Mytilus galloprovincialis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A substance immunologically related to vertebrate glutamic acid decarboxylase (GAD) has been visualized in the pedal ganglion of Mytilus with the pre-embedding peroxidase-antiperoxidase method, by use of an antiserum raised in sheep against rat brain GAD. The results show that GAD-like immunoreactivity is present both in neuronal perikarya and in nerve fibers. Positive neurons are located mainly among the fibers of the ganglion neuropil at the commissural level, and more rarely close to unreactive cortical cell bodies. Immunoreactive nerve fibers are observed throughout the neuropil and also in cerebropedal and pedal nerves.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00212538
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  • 62
    Digitale Medien
    Digitale Medien
    Redistribution of cell surface transferrin receptors prior to their concentration in coated pits as revealed by immunoferritin labels (1986)
    Springer
    Cell & tissue research 244 (1986), S. 613-619 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Cell-surface transferrin receptor ; Redistribution of cell-surface receptors ; Receptor-mediated endocytosis ; K562 cells ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Immunocytochemistry has been used to study distribution of cell surface transferrin receptors in erythroid, leukemic (K562) cells. The cells were fixed and labelled with monoclonal (OKT-9) anti-transferrin receptor antibodies; the antibody-labelled receptors were then detected by either immunofluoresceinor immunoferritin-antimouse-antibody conjugates. Typically, the immunoferritin labels were distributed diffusely at the non-coated regions of the cell surface as well as concentrated in the clathrincoated pits. To examine further this pattern of distribution, cells were labelled at 0° C and then warmed to 37° C for zero to 30 min prior to fixation. The majority of the immunoferritin labels were initially dispersed in small groups at the non-coated regions of the cell surface (mean = 6 immunoferritin labels/cluster), but larger groups were common subsequent to incubation at 37° C (mean = 13 immunoferritin labels/cluster). However, the size of immunoferritin labels in the coated pits was unchanged (mean = 12 immunoferritin labels/pit). Immunoferritin labels were typical in coated and uncoated vesicles l min after warming to 37° C, but common in endosomes, multivesicular bodies and lysosomes by 30 min. It appears that single cell-surface receptors form large aggregates prior to their concentration in coated pits. Coated vesicles, uncoated vesicles, and endosomal vacuoles may together form the non-lysosomal compartment where the internalized receptors might be dissociated from the ligands (antibodies).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00212541
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  • 63
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of S-100 protein in stellate cells (folliculo-stellate cells) of the adenohypophysis in the monkeys Macaca irus and Cercopithecus aethiops (1986)
    Springer
    Cell & tissue research 246 (1986), S. 237-242 
    Details
    ISSN: 1432-0878
    Schlagwort(e): S-100 protein (human, bovine) ; Folliculo stellate cells ; Adenohypophysis ; Immunocytochemistry ; Monkeys (Macaca irus, Cercopithecus aethiops)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary With the use of an antibody against bovine S-100 protein, it was possible to reveal a characteristic cell type in the pars distalis and the pars tuberalis of the monkey Macaca irus. In the adenohypophysis of Cercopithecus aethiops, labeled cells were present in the pars distalis, pars tuberalis, and pars intermedia. These cells, so-called folliculo-stellate cells, were found in all pituitaries studied. Surprisingly, an antibody against human S-100 protein did not label the stellate cells of the adenohypophysis. However, in Macaca irus, this antibody gave a strong positive reaction with various other cell types (interstitial cells of the pineal gland, Müller cells of the retina, autonomic ganglionic cells, glial cells of the central nervous system, Schwann cells, Bergmann glia of the cerebellum, fat cells, reticular cells of lymphoid organs). By use of double immunoenzymatic labeling, it was evident that stellate cells are spatially related either to somatotropes, prolactin cells, “corticotropes”, or to glycoprotein-containing cells. Thus, a specific relationship to a particular endocrine-cell type could not be observed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215885
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  • 64
    Digitale Medien
    Digitale Medien
    5-Hydroxytryptamine immunoreactivity in the epidermal sacciform gland cells of the clingfish Lepadogaster candollei Risso (1986)
    Springer
    Cell & tissue research 246 (1986), S. 679-682 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Fish skin ; Sacciform glandular cells ; Immunocytochemistry ; 5-Hydroxytryptamine ; Lepadogaster candollei
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Serotonin has been demonstrated in the epidermal sacciform glandular cells of the clingfish Lepadogaster candollei by use of immunocytochemistry. Serotonin immunoreactivity is found both in the peripheral cytoplasm of the glandular cells and their luminal secretion. The presence of serotonin in the sacciform glandular cells parallels that located by both biochemical and immunocytochemical procedures in the cutaneous glands of many amphibian species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215211
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  • 65
    Digitale Medien
    Digitale Medien
    Immunocytochemical study of the development of vasotocin/mesotocin in the hypothalamo-hypophysial system of the chick embryo (1986)
    Springer
    Cell & tissue research 243 (1986), S. 15-31 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Embryonic hypothalamo-hypophysial system ; Vasotocin neurons ; Mesotocin neurons ; Chick embryo hypothalamus ; Immunocytochemistry ; Chicken
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The hypothalamo-hypophysial system of the chick embryo has been studied with a monoclonal antibody which cross-reacts with arginine vasotocin and mesotocin, using thick (100 μm) sections in conjunction with a peroxidase-conjugated rabbit anti-mouse antibody. Although weakly stained perikarya occur occasionally in the tuberal region on embryonic days 6 and 7, the most consistent immunostaining of perikarya is found in the periventricular region of the caudal midhypothalamus at the level of the optic chiasm after embryonic day 8 1/2. Synthesis of peptides, therefore, takes place while the cells are close to their site of origin. Between embryonic days 9 and 10, beaded axons run along the anterior median eminence closely apposed to the adenohypophysis, thereby forming the anlage of the zona externa. The axons of the hypothalamo-neurohypophysial tract surround the neural lobe between embryonic days 11 and 12. The caudal to rostral wave of neuronal maturation that occurs during development appears to be due to a progressive differentiation of the periventricular zone, as well as the migration of perikarya. The early periventricular perikarya at embryonic day 8 1/2 send processes rostrally in a wing-shaped formation that extends both dorso- and ventrolaterally. From embryonic days 10 to 12, perikarya can be observed in the wing-like extensions, apparently migrating to rostral levels. The dorsolateral pathway gives rise at its midportion to the lateral cell group, whereas those perikarya migrating more laterally form the anlage of the external supraoptic nucleus. The ventrolateral wing-shaped extension of perikarya appears to be directed toward the ventral group and those lateral perikarya continuous with it. The location of mature neuronal cell groups is well established by embryonic day 17.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221848
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  • 66
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of ornithine decarboxylase in cultured murine cells (1986)
    Springer
    Cell & tissue research 243 (1986), S. 33-40 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Ornithine decarboxylase ; Macrophage ; Immunocytochemistry ; Murine cell culture ; Antibody specificity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Antiserum elicited to ornithine decarboxylase (ODC) purified from murine RAW 264 macrophage-like cells has been employed to localize ODC in cultured murine cells. The antiserum immunoprecipitated 100% of the ODC activity from the cultured cells. The specificity of the antiserum was demonstrated by the immunoprecipitation from 35S-methionine metabolically-labeled cell extracts of a single protein which migrated upon SDS-gel electrophoresis coincident with authentic ODC. Indirect immunofluorescence experiments were performed on paraformaldehyde-fixed RAW 264 cells and JB6 epidermal cells using the rabbit anti-ODC antiserum and FITC-conjugated goat anti-rabbit IgG. Little immunofluorescence was apparent in non-stimulated cells. Intense immunofluorescence was detectable in stimulated cells at times of peak cellular ODC activity. Antigenically-reactive ODC was localized diffusely in the cytoplasm and was absent in the nuclei of RAW 264 cells, whereas in the JB6 cells the immunodetectable enzyme protein was localized in a punctate pattern in both the cytoplasm and nucleoplasm and was absent in the nucleolus. The appearance and disappearance of immunoreactive ODC in both cell types after stimulation was consistent with the alterations in ODC activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221849
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  • 67
    Digitale Medien
    Digitale Medien
    Immunoreactivity of the hermaphroditic gonad of the snail Helix aspersa Müller towards antibodies raised to fragments of pre-pro-opio-melanocortin (1986)
    Springer
    Cell & tissue research 245 (1986), S. 337-341 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; Hermaphrodite gonad ; Pre-pro-opio-melanocortin ; FMRF-amide-like materials ; Snail, Helix aspersa Müller
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Male and female germinal cells of the active hermaphroditic gonad of a snail (Helix aspersa Müller) reveal a positive immunoreactivity to some antibodies raised against biologically active peptides related to pre-pro-opiomelanocortin of vertebrates. All but the oldest cells of the different spermatogenetic and oogenetic stages are methionine-enkephalin-immunopositive, whereas only the young oocytes are α-MSH- and 17–39 ACTH-positive. Sometimes some male cells show an 1–24 ACTH positivity. Structures other than germinal cells also react with some antibodies: for example, the nurse cells are β-MSH-immunoreactive, the nerve fibers surrounding each acinus and the hermaphrodite duct are both 17–39 ACTH and FMRF amide positive.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213940
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  • 68
    Digitale Medien
    Digitale Medien
    Differential immunocytochemical staining for glial fibrillary acidic (GFA) protein, S-100 protein and glutamine synthetase in the rat subcommissural organ, nonspecialized ventricular ependyma and adjacent neuropil (1986)
    Springer
    Cell & tissue research 245 (1986), S. 343-351 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Ependyma ; Astrocytes ; Immunocytochemistry ; Glial fibrillary acidic (GFA) protein ; S-100 protein ; Glutamine synthetase ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Antibodies raised against glial fibrillary acidic protein (GFA), S-100 protein (S100) and glutamine synthetase (GS) are currently used as glial markers. The distribution of GFA, S100 and GS in the ependyma of the rat subcommissural organ (SCO), as well as in the adjacent nonspecialized ventricular ependyma and neuropil of the periaqueductal grey matter, was studied by use of the immunocytochemical peroxidase-antiperoxidase technique. In the neuropil, GFA, S100 and GS were found in glial elements, i.e., in fibrous (GFA, S100) and protoplasmic astrocytes (S100, GS). The presence of S100 in the majority of the ventricular ependymal cells and tanycytes, and the presence of GFA in a limited number of ventricular ependymal cells and tanycytes confirm the glial nature of these cells. The absence of S100, GFA and GS from the ependymocytes of the SCO, which are considered to be modified ependymal cells, suggests either a non-astrocytic lineage of these cells or an extreme specialization of the SCO-cells as glycoprotein-synthesizing and secreting elements, a process that may have led to the disappearance of the glial markers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213941
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  • 69
    Digitale Medien
    Digitale Medien
    Immunocytochemical identification of structures containing putative red pigment-concentrating hormone in two species of decapod crustaceans (1986)
    Springer
    Cell & tissue research 245 (1986), S. 377-386 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Red pigment-concentrating hormone ; Immunocytochemistry ; Neurosecretion ; Crustaceans ; Carcinus maenas ; Orconectes limosus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary By use of an antiserum raised against the Nterminal sequence pGlu-Leu-Asn-Phe..., common to red pigment-concentrating hormone (RPCH) of Pandalus borealis and three structurally similar insect neuropeptides, putative RPCH-immunopositive structures were revealed in the eyestalks of Carcinus maenas and Orconectes limosus and in the brain and thoracic ganglion (TG) of C. maenas. In the eyestalks, complete neurosecretory pathways were demonstrated, consisting of perikarya, axons and terminals in the neurohemal organ, the sinus gland (SG). In C. maenas approximately 20 small RPCH cells are present as a distinct group adjacent to the medulla terminalis ganglionic X-organ (MTGXO, XO). They are morphologically different from the larger XO perikarya, which contain the crustacean hyperglycemic hormone (CHH). The occurrence of both neuropeptides in distinct neurosecretory pathways was ascertained by immunologic double staining (PAP/gold) or by analysis of consecutive sections. In addition, a group of two to four larger RPCH cells is located in the proximal part of the MT. In O. limosus, RPCH cells are found in the XO. Cells corresponding to the proximal MT cells of C. maenas were not found. In both species, a few more weakly staining immunopositive perikarya were observed in clusters of cell somata of the optic ganglia. It is uncertain whether these are connected to the SG. In the brain of C. maenas, several smaller and three larger perikarya were consistently observed in the dorsal lateral cell somata adjacent to the olfactory lobes. In the optic nerve, two axons that project into the eyestalk were stained. Some axons were also observed in the ventral median neuropil of the brain. In the TG, RPCH cells were found in small numbers in median positions, i.e., in clusters of somata between the ganglia of the appendages. HPLC analysis of the red pigment-concentrating activity from the SG of C. maenas revealed that the retention time of the neuropeptide is similar but not identical to that of Pandalus borealis RPCH.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213945
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  • 70
    Digitale Medien
    Digitale Medien
    Choline acetyltransferase-like immunoreactivity in the brain of Drosophila melanogaster (1986)
    Springer
    Cell & tissue research 246 (1986), S. 57-62 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Insect brain ; Neurotransmitters ; Immunocytochemistry ; Drosophila melanogaster
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Using a monoclonal antibody selective for the acetylcholine (ACh)-synthesizing enzyme choline acetyltransferase (ChAT) of Drosophila melanogaster we find ChAT-like immunoreactivity in specific synaptic regions throughout the brain of Drosophila melanogaster apart from the lobes and the peduncle of the mushroom body and most of the first visual neuropile (lamina). Several anatomically well-defined central brain structures exhibit particularly strong binding. Characteristic differential staining patterns are observed for each of the four neuromeres of the optic lobes. Cell bodies appear not to bind this antibody. The prominent features of the distribution of ChAT-like immunoreactivity are paralleled by the distribution of acetylcholine hydrolyzing enzymatic activity as revealed by histochemical staining for acetylcholine esterase (AChE). These results are discussed in comparison with published data on enzyme distribution, choline uptake and ACh receptor binding in the nervous system of Drosophila melanogaster.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218999
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  • 71
    Digitale Medien
    Digitale Medien
    Mosquito trypsin: immunocytochemical localization in the midgut of blood-fed Aedes aegypti (L.) (1986)
    Springer
    Cell & tissue research 245 (1986), S. 19-27 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Trypsin ; Immunocytochemistry ; Midgut ; Exocytosis ; Aedes aegypti
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A polyclonal antibody was raised against trypsin purified from the midgut of blood-fed Aedes aegypti. Using this antibody and our modification of the peroxidase-antiperoxidase immunocytochemical reaction, strong activity was found in the lumen of the midgut at the light-microscopical level. The activity was localized mainly in the posterior part of the distensible, abdominal midgut, along the periphery of the blood bolus and within the peritrophic membrane. Immunoreactivity appeared 8 h after the blood meal and was most prominent around 24 h, coinciding with our previous spectrophotometric determinations of trypsin. At the electron-microscopical level, secretory granules, immunocytochemically labelled with anti-trypsin antibody and protein A-colloidal gold, were first detected about 12 h after the blood meal. At 18 h, the secretory pathway could be followed immunocytochemically from the formation of granules in the Golgi complex until their release by exocytosis in the midgut lumen. By 24 h, there was a reduction in secretory granules, and large lysosomes appeared. The process of secretion described for this mosquito is comparable to similar events in vertebrate secretory systems and the presence of an intracellular trypsinogen is suggested.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218082
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  • 72
    Digitale Medien
    Digitale Medien
    Somatotrophs and lactotrophs in the anterior pituitary of fetal and neonatal rats (1986)
    Springer
    Cell & tissue research 245 (1986), S. 457-460 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Anterior pituitary ; Somatotroph ; Lactotroph ; Ultrastructure ; Immunocytochemistry ; Fetal and neonatal rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ultrastructure of immunoreactive somatotrophs and lactotrophs in pituitaries of fetal rats at 19, 20 and 21 days of gestation and on the day of birth was studied. Somatotrophs, first detectable at 19 days of gestation, undergo only minor modifications before reaching the structure described for adults. In particular there is an increase in the endoplasmic reticulum and Golgi apparatus. Lactotrophs, first identifiable in newborn rats, are very different in ultrastructure from adult cells, because the secretory granules are generally small, but variable in shape and size, and the Golgi complex is prominent.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213955
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  • 73
    Digitale Medien
    Digitale Medien
    Localization and function of an FMRFamide-like substance in the aorta of Helix aspersa (1986)
    Springer
    Cell & tissue research 246 (1986), S. 303-307 
    Details
    ISSN: 1432-0878
    Schlagwort(e): FMRFamide ; Aorta ; Bioassay ; Immunocytochemistry ; Helix aspersa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary With an antiserum (aFM) against the molluscan “cardio-active” FMRFamide (Phe-Met-Arg-Phe-NH2) numerous immunoreactive axons were found in the outer, longitudinal, muscle layer of the anterior aorta of Helix aspersa. Immunoreactive axons were rare in the inner, circular, muscle layer. At the ultrastructural level four types of axons could be distinguished. The granules containing the immunoreactive substance (mean diameter ca. 100 nm) are present in type-2 axons. The effect of synthetic FMRF-amide was tested in vitro on preparations of ring- and tubule-shaped pieces of the anterior aorta. Physiological doses (3 × 10-7 M) provoked contractions of the circular muscle fibres, but had no effect on the longitudinal muscle cells. Apparently in vivo the FMRF-like substance diffuses from the richly innervated longitudinal muscle layer to the circular muscle layer, where it exerts its effect. This conclusion is sustained by the observation that the contents of the aFM-immunoreactive granules in type-2 axons are released by exocytosis in a “non-synaptic” fashion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215892
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  • 74
    Digitale Medien
    Digitale Medien
    Light and electron-microscopic immunocytochemistry and lectin histochemistry of the subcommissural organ: Evidence for processing of the secretory material (1986)
    Springer
    Cell & tissue research 243 (1986), S. 545-559 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Glycoproteins ; Secretory process ; Immunocytochemistry ; Lectin histochemistry ; Rat ; Vertebrates
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The subcommissural organ (SCO) of the rat was investigated by use of histochemical and immunocytochemical methods at the light and electron-microscopic levels. Consecutive thin methacrylate sections were stained with the pseudoisocyanin (Psi), immunoperoxidase (IMC; employing an antiserum against Reissner's fiber, AFRU), periodic acid-Schiff (PAS) and periodic acid-silver methenamine (SM) techniques, and reacted with six types of lectins. Psi, SM, concanavalin A (Con A) and IMC were also used for double and triple sequential staining of the same section. Increasing dilutions of AFRU (from 1∶1000 to 1∶200 000) were used for immunostaining of serial paraffin sections. In addition, ultrastructural localization of (i) Con A-binding sites and (ii) immunoreactive secretory material was performed. Some of these procedures were also applied to the ophidian and canine SCO. Con A-positive, Psi-positive and immunoreactive materials coexisted within the same cisternae of the rough endoplasmic reticulum. The Golgi apparatus lacked Con A-positive and immunoreactive substances. Apical secretory granules and secreted material lying on the surface of the SCO showed (i) the highest affinity for AFRU, but were (ii) Con A-negative, and (iii) wheat-germ agglutinin-, PAS and SM-positive. Reissner's fiber displayed a low affinity for AFRU. It is suggested that the SCO secretes N-linked glycoproteins, the carbohydrate and protein moeities of which undergo (i) a maturation process before being released, and (ii) some kind of modification(s) after their release into the ventricle. The perivascular secretory cells of the dog SCO might secrete a material different from that secreted by the ependymal cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218061
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  • 75
    Digitale Medien
    Digitale Medien
    Correlative immuno-electron-microscopic and immunofluorescent localization of actin in sensory and supporting cells of the inner ear by use of a low-temperature embedding resin (1986)
    Springer
    Cell & tissue research 245 (1986), S. 229-235 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Inner ear ; Cochlea ; Actin ; Immunocytochemistry ; Chinchilla
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The cochleas from chinchilla inner ears were processed in the cold through Lowicryl K4M, and cured by UV light. Thick (2 μm) sections were reacted with primary antibodies raised against actin, and anti-actin antibodies localized by FITC epifluorescence. On thin sections from the same blocks anti-actin antibodies were localized ultrastructurally with secondary antibodies coupled to colloidal gold. In the hair cells, actin was present in the stereocilia and cuticular plate, regions where thin filaments were observed by electron microscopy. Colloidal gold was uniformly distributed over these regions and over the stereocilia rootlets demonstrating that actin was present in this region although previously in permeabilized cells, the rootlet was not decorated with myosin subfragment S-1. Actin was present in the pillar and Deiters supporting cells at the reticular lamina and at the basilar membrane, where a meshwork of thin filaments was seen by electron microscopy. Colloidal gold particles were also localized over the thin processes of the pillar and Deiters cells, and over the region of the Deiters cell which envelops the base of the outer hair cell. In these regions actin co-localized with microtubules along the entire length of the supporting cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213926
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  • 76
    Digitale Medien
    Digitale Medien
    Immunocytochemical study of fibronectin in the sea lamprey, Petromyzon marinus, and the Atlantic hagfish, Myxine glutinosa (1986)
    Springer
    Cell & tissue research 244 (1986), S. 549-555 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Fibronectin ; Lamprey ; Hagfish ; Immunocytochemistry ; Connective tissues
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Immunoreactive fibronectin-like material was localized within tissues of agnathans (hagfishes and lampreys) by an immunoperoxidase technique. Fibronectin was detected in basement membranes and in loose and dense connective tissues throughout the agnathan body. A fibronectin-like component was also identified in the plasma of both lampreys and hagfishes. The results indicate that fibronectin or a fibronectin-like material is a major component of agnathan connective tissues. Although there were some variations in the localization of fibronectin both between the lamprey and the hagfish and between agnathan and other vertebrate tissues, the generalized pattern of distribution of fibronectin in the agnathans supports the view that this protein, like that in higher vertebrates, plays a role in cellmatrix adhesion and tissue organization.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00212532
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  • 77
    Digitale Medien
    Digitale Medien
    Ultrastructure and central projections of extraocular photoreceptors in caddiesflies (Insecta: Trichoptera) (1986)
    Springer
    Cell & tissue research 245 (1986), S. 643-648 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Photoreceptors, extraocular ; Optic lobes ; Stemmata ; Immunocytochemistry ; Insecta (Trichoptera)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Retained larval eyes (stemmata) were studied in the imagines of three species of Trichoptera: Phrygania grandis, Agrypnia varia, and Trichostegia minor. At the light-microscopic level the stemmata of all three species appeared to represent different stages of reduction with respect to size, shape and number of lenses. However, in all three species electron-microscopic studies showed units with monolayered rhabdoms, each formed by four retinula cells. By use of immunocytochemistry the presence of S-antigen was demonstrated in the retinula cells and their axons. This method also revealed the central projections of the axons of the retinula cells, which were found (i) to terminate either in the lamina accessoria or (ii) to penetrate this area to join the fibers of the outer chiasma of the optic lobes and then terminate in the medulla accessoria. The lamina accessoria and the medulla accessoria are the assumed remnants of the larval optic lobes. It is suggested that the imaginal stemmata might still be functioning photoreceptors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218567
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  • 78
    Digitale Medien
    Digitale Medien
    Localization of enkephalin immunoreactivity in diverse tissues and cells of the developing and adult rat (1986)
    Springer
    Cell & tissue research 246 (1986), S. 561-565 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Growth ; Enkephalin ; Endogenous opioids ; Immunocytochemistry ; Differentiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of enkephalin, an endogenous opioid, in tissues and cells of the developing and adult rat was determined by immunocytochemistry with antibodies to met- and leu-enkephalin. Met- and leu-enkephalin were found in all developing cells investigated, with staining generally located throughout the cytoplasm; cell nuclei were not immunoreactive. In comparison to developing cells, immunoreactive analogues to met-enkephalin were usually difficult to detect in the adult. Some notable exceptions were reaction products in leukocytes in blood, lung, and cortex of thymus, fibroblasts in the skin, and seminiferous tubules. These results, in concert with earlier reports that opioid receptors are found largely in developing, but not adult, tissues, indicate that endogenous opioids are specifically involved in biological development, particularly cell proliferation and differentiation. Immunoreactivity in adult nonneural cells may be related to their development in some cases, but also could indicate other functions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215197
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  • 79
    Digitale Medien
    Digitale Medien
    Localization of substance P-like immunoreactive fibers in the thoracic spinal cord of guinea pig (1986)
    Springer
    Cell & tissue research 246 (1986), S. 653-665 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Substance P ; Spinal cord ; Dorsal-horn fiber system ; Immunocytochemistry ; Guinea pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A dorsal-horn fiber system is revealed in the thoracic spinal cord of guinea pig by means of substance P immunocytochemistry. This system has repeated craniocaudal and/or caudo-cranial extensions and possesses five main components: (1) a superficial network, situated beneath the dorsolateral surface of the spinal cord. This network is connected with the dorsal root fibers and the accumulations of substance P-like immunoreactive (SP-LI) fibers in the Lissauer's tract; (2) an accumulation of SP-LI fibers in the Lissauer's tract at the border of the dorsal horn; (3) two collateral SP-LI fascicles (one lateral and one medial) emerging from the SP-LI fiber accumulation in the Lissauer's tract; (4) a transversal fascicle running through laminae III–V, and (5) an SP-LI network in the region of the lateral spinal cord nucleus. These components of the dorsal-horn fiber system show widespread connections with ipsi-and contralateral spinal cord areas, connecting them in cranio-caudal and/or caudo-cranial directions. The SP-LI dorsal-horn system has close relationship with groups of preganglionic sympathetic cells in the intermediate zone of the spinal cord, respective with the vegetative network of this zone. It is suggested that some fibers of the dorsal-horn system that originate from dorsal-root ganglia may represent primary sensory or visceral afferents. It is likely that the dorsal-horn fiber system and the vegetative network of the thoracic spinal cord may represent the morphological basis for the integration of (1) the central and peripheral vegetative nervous systems, and (2) the somatic and vegetative nervous system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215208
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  • 80
    Digitale Medien
    Digitale Medien
    Differential subcellular localization of ACTH and α-MSH in corticotropes of the rat anterior pituitary (1986)
    Springer
    Cell & tissue research 243 (1986), S. 229-238 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Anterior pituitary ; Corticotropes ; α-MSH ; Immunocytochemistry ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Specific antisera to α-melanotropin (α-MSH) and corticotropin (ACTH 1-39) were used to obtain immunocytochemical evidence for the differential localization of α-MSH and ACTH in the secretory granules of corticotropes of rat anterior pituitary. The specificity of the antisera was established by binding 131I-labeled α-MSH and ACTH 1-39 to their respective antisera. Double-labeling immunocytochemistry (for α-MSH, ferritin; for ACTH, colloidal gold) was performed. Some secretory granules were labeled with ferritin particles (α-MSH), whereas others contained gold particles (ACTH). Only a few granules showed both ACTH and α-MSH. In typical corticotropes (stellate in form with a small number of secretory granules aligned along the cell periphery) only some of the secretory granules that were labeled with anti-ACTH serum were also immunoreactive to anti-α-MSH. In atypical corticotropes (polygonal in shape and containing a large number of secretory granules) almost all of the immunoreactive ACTH secretory granules were also positive to anti-α-MSH serum. An intermediate type of corticotrope was observed containing a small number of secretory granules, almost all of which were labeled with anti-α-MSH. Thus, rat anterior pituitary corticotropes may be classified into three types according to the distribution and content of α-MSH. The light-microscopic immuncytochemistry provided similar results.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00251036
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  • 81
    Digitale Medien
    Digitale Medien
    The organization of serotonin-immunoreactive neuronal systems in the brain of the crested newt, Triturus cristatus carnifex Laur. (1986)
    Springer
    Cell & tissue research 243 (1986), S. 239-247 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Serotonin ; Indoleamines ; Aminergic neurons ; Immunocytochemistry ; Central nervous system ; Crested newt (Triturus cristatus carnifex)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of serotonin (5-HT) immunoreactive structures has been investigated in the brain of the crested newt by means of indirect immunofluorescence, and unlabeled antibody peroxidase-antiperoxidase-complex (PAP) or biotin-avidin-system (BAS) techniques. In the newt, the bulk of the serotoninergic system extends from the raphe region of the medulla oblongata, through the isthmus, toward the mesencephalic tegmentum, and is characterized by pyriform neurons mainly located in a subependymal position, close to the midline. Also in the caudal hypothalamus, in addition to some 5-HT-positive adenohypophysial cells, many immunoreactive CSF-contacting neurons are found lining the paraventricular organ and the nucleus infundibularis dorsalis. A rich serotoninergic innervation was observed in the preoptic area and in the habenular complex. Concerning the telencephalon, immunopositive nerve fibers are encountered in the dorsal pallium, primordium hippocampi, striatum and olfactory bulbs. The general organization of serotoninergic systems in the newt brain exhibit close similarities to that described in higher vertebrates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00251037
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  • 82
    Digitale Medien
    Digitale Medien
    Immunocytochemical evidence for the presence of “mammalian” neurohormonal peptides in neurones of the tapeworm Diphyllobothrium dendriticum (1986)
    Springer
    Cell & tissue research 243 (1986), S. 41-49 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neuropeptides ; Serotonin ; Diphyllobothrium dendriticum ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In the nervous system of the obligatory endoparasite Diphyllobothrium dendriticum immunoreactivity (IR) to growth hormone-releasing factor (GRF), peptide histidine isoleucine (PHI), bovine pancreatic polypeptide (BPP), gastrin, gastrin-releasing peptide (GRP), oxytocin, FMRF-amide (FMRF) and serotonin (5HT) was demonstrated by immunocytochemical methods. A very strong GRF-IR was observed in the CNS and PNS of larvae and of the constantly growing adult worms. GRF-IR axon terminals occur beneath the basal lamina of the tegument along the inside of the bothridia, the holdfast organ of the worm. GRF-IR fibres surround the yolk producing vitelline glands and occur in the wall of the vagina. PHI-IR was observed in the CNS and PNS of larvae and adult worms. PHI-IR terminals occur beneath the basal lamina of the tegument along the strobila, the nutrient absorbing surface of the worm. PHI-IR fibres seem to innervate the testicular follicles. FMRF-IR fibres and perikarya occur close to the vitelline glands and the uterine pore and in the male copulatory organ. Numerous large 5HT-IR perikarya with long varicose fibres were observed in the nervous system of the worm. 5HT-IR perikarya occur close to the genital atrium. D. dendriticum is the phylogenetically lowest organism in which IR to PHI has been demonstrated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221850
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  • 83
    Digitale Medien
    Digitale Medien
    Immunocytochemical differentiation between adipokinetic hormone (AKH)-like peptides in neurons and glandular cells in the corpus cardiacum of Locusta migratoria and Periplaneta americana with C-terminal and N-terminal specific antisera to AKH (1986)
    Springer
    Cell & tissue research 243 (1986), S. 9-14 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Adipokinetic hormone ; Corpus cardiacum ; Locusta migratoria ; Periplaneta americana ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary An immunocytochemical method was used to differentiate between immunoreactive substances in glandular cells in the corpora cardiaca (CC) and in certain cerebral neurons in 2 insect species, Locusta migratoria migratorioides and Periplaneta americana. The staining properties of antisera raised to different parts of the decapeptide adipokinetic hormone (AKH) were compared and their specificity was determined by preabsorption with AKH and related peptides. Antibodies raised to the N-terminal part of AKH (serum 433) and the central and C-terminal part (serum 241) were found to have different staining properties. In the CC of the locust both antisera show a strong immunoreactivity with glandular cells, we therefore suggest that at least one of the compounds revealed is AKH. Some of the glandular cells in the locust and large numbers of glandular cells in the CC of the cockroach are revealed by the N-terminal specific antiserum. On the other hand, neurons in the central nervous system are revealed only by the C-terminal specific antiserum. The possible identity of the various substances revealed by these two antisera is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221847
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  • 84
    Digitale Medien
    Digitale Medien
    Comparative immunocytochemical study of release sites of insulin, glucagon and AKH-like products in Locusta migratoria, Periplaneta americana, and Carausius morosus (1986)
    Springer
    Cell & tissue research 245 (1986), S. 267-271 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Peripheral neurosecretory structures ; Immunocytochemistry ; Insulin ; Glucagon ; AKH ; Insects
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Insulin, glucagon and adipokinetic hormone antisera were applied to the corpora cardiaca, perisympathetic organs, neurohemal areas and peripheral neurosecretory cells of three insect species, the locust Locusta migratoria, the cockroach Periplaneta americana, and the stick insect Carausius morosus. The neurohemal part of the corpora cardiaca was shown to be immunoreactive to both insulin and glucagon antisera while the glandular cells reacted to adipokinetic hormone antisera. The perisympathetic organs seem to be devoid of these three substances, but certain peripheral neurohemal areas contained AKH and glucagon immunoreactive products. The latter were found to originate in the peripheral neurosecretory cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213931
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  • 85
    Digitale Medien
    Digitale Medien
    Immunocytochemical demonstration of 5-HT-like and FMRF-amide-like substances in whole mounts of Microstomum lineare (Turbellaria) (1986)
    Springer
    Cell & tissue research 246 (1986), S. 7-12 
    Details
    ISSN: 1432-0878
    Schlagwort(e): 5-HT ; FMRF-amide ; Immunocytochemistry ; Nervous system ; Microstomum lineare (Turbellaria)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A whole mount immunofluorescence method is used for an investigation of immunoreactivity (IR) to anti-(a-) 5-HT and anti-(a-)FMRF-amide in the nervous system (NS) of Microstomum lineare (Turbellaria, Macrostomida, Platyhelminthes). New details of the organization of the NS are demonstrated, differences in 5-HT and FMRF amide IR are revealed, and new information on the development of the NS in zooids is obtained. In contrast to previous reports of a reduction (one pair of nerve cords without transverse processes) of the basic turbellarian plan, IR to both antisera reveals three pairs of longitudinal nerve cords, and features of the orthogonal organization, characterized by transverse commissures. The lateral pair of nerve cords is the most prominent. The following differences in the patterns of 5-HT and FMRF-amide IR are observed: 1. Perikarya positive for a-5-HT and a-FMRF-amide in the brain show different localizations. 2. Perikarya positive for a-5HT occur along the main lateral nerve cords, while the cords visualized by FMRF-amide IR look double-stranded and lack associated perikarya. 3. 5-HT IR is observed in a postpharyngeal commissure, which is absent in the a FMRF-amide-stained preparations. 4. In developing zooids 5-HT IR is first observed in the postpharyngeal commissure and later on in an increasing number of perikarya and in the neuropile. The first FMRF-amide IR in developing zooids appears in the cerebral commissure and in two perikarya in front of this commissure.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218992
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  • 86
    Digitale Medien
    Digitale Medien
    Immunocytochemical demonstration of growth hormone, prolactin and somatostatin-like immunoreactivities in the brain of larval, young adult and upstream migrant adult sea lamprey, Petromyzon marinus (1986)
    Springer
    Cell & tissue research 246 (1986), S. 23-31 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Growth hormone ; Prolactin ; Somatostatin ; Immunocytochemistry ; Lamprey
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Growth hormone, prolactin and somatostatinlike immunoreactivities were demonstrated in the brains of larval, young adult (parasitic) and upstream migrant adult sea lampreys, Petromyzon marinus, by means of immunoperoxidase techniques. Growth hormone (GH) and prolactin (PRL) were observed within separate perikarya in the nucleus praeopticus, within fibers in the commissura praeinfundibularis, and in nerve endings within the neurohypophysis of larval and adult-stage lampreys. Cell bodies demonstrating immunoreactive growth hormone were more numerous than those reactive for prolactin. Unlike in the upstream migrant adult lamprey, no GH or PRL was demonstrated in the adenohypophysis of larval or parasitic lamprey. Somatostatin (SRIF)-like immunoreactive neurons were demonstrated in the nucleus commissurae praeinfundibularis, anterior and posterior pars ventralis hypothalami, pars dorsalis thalami, and the tegmentum motorium rhombencephali of larval, parasitic and upstream migrant adult lampreys. Many of the SRIF containing neurons within the hypothalamus were cerebrospinal fluid (CSF)-contacting cells. SRIF fibers were found throughout most of the brain predominating within the nucleus praeopticus, pars ventralis hypothalami, and the nucleus interpeduncularis. No SRIF immunoreactivity was found within the neurophyophysis. The possible functions of these peptides within the brain of the lamprey are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00218994
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  • 87
    Digitale Medien
    Digitale Medien
    The shape and distribution of astrocytes in the retina of the adult rabbit (1986)
    Springer
    Cell & tissue research 246 (1986), S. 91-102 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Retina ; Astroglia ; Perivascular glia ; Glial fibrillary acidic protein (GFAP) ; Nerve fiber layer ; Immunocytochemistry ; Rabbit
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Astrocytes stained by antibodies to glial fibrillary acidic protein (GFAP) were examined in whole-mount preparations of retinae from adult rabbits and found to be restricted to the medullary rays. Astroglial cells exhibited a variety of shapes that varied between two extreme morphologies. One extreme was an astrocyte that possessed a few sturdy primary processes as well as finer processes and was strongly GFAP positive. The other extreme was an astroglial cell that displayed a star-shaped appearance; its perikarya gave rise to a few thin, radially oriented processes, which were rather weakly GFAP positive. The majority of astroglial processes were aligned with the ganglioncell axons, but some of their processes were in contact with capillaries. It has been proposed that astrocytes are specifically associated with ganglion-cell axons. Their restriction to the medullary rays in the retina of the rabbit suggests, however, that their physiological role is also concerned with the vascular system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219004
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  • 88
    Digitale Medien
    Digitale Medien
    Deletion polymorphism in a Drosophila melanogaster heat shock gene (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 266-272 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Heat shock ; Polymorphism ; Transcript mapping ; Deletion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have continued the transcriptional analysis of the region of cytological locus 67B that contains the four small heat shock genes and other genes. Transcription from one of the heat shock genes in the region, hsp 26, takes place during high temperature treatment and at certain developmental stages, without heat shock, in several tissues, such as imaginal discs and adult ovaries. Observations of unexpected products after nuclease protection experiments periments provided the first indication of what genomic blot experiments showed to be small deletions. The alleles containing the deletion are expressed at the same level as the wild type allele. The deletion shortens the protein product, implying that it is in the coding region. Furthermore, flies homozygous for one of the deletion alleles are viable.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00425508
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  • 89
    Digitale Medien
    Digitale Medien
    Sequence analysis of a yolk protein secretion mutant of Drosophila melanogaster (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 557-560 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Secretion mutant ; Sequence analysis ; Yolk protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The female-sterile mutants fs(1) 1163 of Drosophila melanogaster described by Gans et al. (1975) has been characterised as a yolk protein 1 (YP1) secretion mutant (Bownes and Hames 1978b; Bownes and Hodson 1980). We have cloned and sequenced the YP1 gene from this strain, and the strain in which the mutant was induced. One amino acid substitution was found in the predicted polypeptide sequence, an isoleucine to asparagine change at position 92. The sequence of the leader peptide was identical to previously published YP1 sequences. The possible effects of the amino acid change were investigated by computer analysis, which suggests there is no major alteration of secondary structure, but that a hydrophobic region in YP1 is lost in the mutant. This may affect higher order structure.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00338098
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  • 90
    Digitale Medien
    Digitale Medien
    Visualization of cytoskeletal changes through the life cycle inAcetabularia (1986)
    Springer
    Protoplasma 134 (1986), S. 30-42 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Acetabularia ; Actin ; Cyst formation ; Immunocytochemistry ; Microtubules ; Plant cytoskeleton
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The cytoskeleton in the siphonous, marine green algaAcetabularia is visualized by immunocytochemistry using antibodies against plant alfa tubulin and animal smooth muscle actin. In the vegetative phase of the life cycle, when the cell grows a cylindrical stalk and until the reproductive cap is completed, actin forms continuous, parallel bundles that extend through the entire length of the stalk and cap rays respectively. Microtubules (MTs) cannot be detected until the primary nucleus, located in the rhizoid of the giant cell, divides to form thousands of secondary nuclei. MTs can then be seen radiating from each secondary nucleus that is encountered in the stalk on its migration upwards into the cap rays. They are oriented mostly parallel to the long axis of the cell. At arrival in the cap rays up to the “white spot” stage, when nuclei assume equidistant positions in the cap ray cytoplasm, a radiating system of MTs forms around each nucleus and dramatically increases until impressive radial arrays have developed. This phase coincides with a disappearance of actin bundles in the cap rays, but they are retained in the stalk cytoplasm. Shortly after that additional MTs appear around the disk like partitions of cap ray cytoplasm. Concomitantly, bundles of actin reappear colinearly with the circumferrential MTs eventually forming complete rings around each disk of cap ray cytoplasm. During this process the compartments of the future cysts are gradually bulging outwards and simultaneously the rings of actin sink inwards until domes are formed with the nuclei fixed in the top centers of the domes. At this stage the peripheral areas of the radiating MT systems around the nuclei start to break down, whereas the circumferrential MT systems remain intact. Subsequently, the rings of both actin and MTs decrease in diameter, and finally contract to a spot opposite the nucleus, while the cysts continue to develop their oval shape. After the cysts have become separated, they round up and enter several rounds of nuclear divisions. MTs form short radial arrays around each nucleus with minor changes due to a reduction of MTs during division followed by a reappearance after completion of each division. Actin is rearranged in the cysts to a cortical network of randomly oriented, short bundles, that is maintained until gamete formation sets in. These findings accentuate the involvement of Cytoskeletal elements in the key steps of morphogenesis inAcetabularia to an extent that is unknown in higher plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01276373
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  • 91
    Digitale Medien
    Digitale Medien
    Variation in sexual behavior and negative assortative mating inDrosophila melanogaster (1986)
    Springer
    Behavior genetics 16 (1986), S. 307-317 
    Details
    ISSN: 1573-3297
    Schlagwort(e): assortative mating ; sexual selection ; inbreeding ; polymorphism ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract The hypothesis that negative assortative mating occurs as a mechanism limiting inbreeding between genetically related individuals ofDrosophila melanogaster was tested. In order to avoid bias linked to using inbred lines, experiments made use of the F1 hybrid progeny between lines rendered homozygous on chromosomes 1, 2, and 3. No negative assortative mating was found, but significant additive variation was observed between lines for orientation, vibration, copulation latencies, and copulation duration. There was no consistency of results, either among parameters or between sexes from the same line. It is therefore unlikely that the variations observed are due merely to quantitative differences in “vigor”. Since all lines originated from the same wild population, these differences are a possible estimate of natural variation in sexual behavior.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01070806
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  • 92
    Digitale Medien
    Digitale Medien
    The influence of light on pupation height inDrosophila (1986)
    Springer
    Behavior genetics 16 (1986), S. 407-413 
    Details
    ISSN: 1573-3297
    Schlagwort(e): Drosophila ; pupation height ; larval behavior ; light
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract A comparison of pupation height in light and dark was made using 12 species ofDrosophila, representing four species groups and four different ecological backgrounds (temperate-montane forest,virilis group desert,replate group; cosmopolitanmelanogaster group; tropical forest,willistoni group). Light condition has a significant effect on pupation height in only two of the species. In the light,D. montana stays close to the food surface, whileD. melanogaster pupates higher in light than in dark. Light-dependent patterns of pupation response do not correspond to those previously reported for the light-dependent mating response. Considerable interspecific variation exists for pupation height in each species triad, some of which could provide a basis for larval niche separation. Patterns of species differences in the desertrepleta triad are the same in light and in darkness.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01071320
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  • 93
    Digitale Medien
    Digitale Medien
    Identification and comparisons of the yolk polypeptides and the genes which code for them in D. melanogaster sibling species (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 302-309 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Yolk polypeptides ; Yolk protein genes ; Evolution ; In situ hybridisation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The yolk proteins stored in Drosophila, oocytes for utilisation during embryogenesis are an ideal system for studying the regulation of gene expression during development. The 3 major polypeptides found in yolk in D. melanogaster are synthesised in the fat body and ovarian follicle cells and selectively accumulated by the oocyte during vitellogenesis. In order to understand more about their regulation and the mechanism of uptake, studies on other species are necessary. Three yolk polypeptides have previously been identified in the D. melanogaster sibling species (D. melanogaster, D. simulans, D. mauritiana, D. erecta, D. teissieri, D. orena and D. yakuba). In D. melanogaster three genes located on the X chromosome are known to code for these yolk polypeptides. in this study genomic Southern transfers and in situ hybridisation experiments were carried out on the sibling species. Using the three cloned yolk protein genes from D. melanogaster, homologous sequences could be detected in the sibling species. It is suggested that three yolk protein genes occur in each of these species, all being located on the X chromosome, and that two of the genes are very closely linked in these same species. Yolk protein gene-homologous DNA sequences have also been identified in two more distantly related species D. funebris and D. virilis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00425514
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  • 94
    Digitale Medien
    Digitale Medien
    Autonomy of the wingless mutation in Drosophila melanogaster (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 483-486 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; wingless ; Autonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary T(Y;2) translocations were used to cytologically localise the wingless locus of Drosophila melanogaster. We found that an existing T(Y;2), which is an insertion of a segment of 2L into the Y chromosome, has wg + within this insert. This Y chromosome was used to generate an attached XY chromosome containing wg +. The mutation claret-nondisjunctional (ca nd) was used to induce the loss of this XY chromosome and thus generate gynandromorphs with wg 1/wg 1 male tissue and wg +/wg 1/wg 1 female tissue. Analysis of these gynanders demonstrated that a genotypically wingless mutant hemithorax is usually also phenotypically mutant in these half body mosaics; thus wg 1 is discautonomous. This observation is of interest as it is known that wg is not cell autonomous.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00338086
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  • 95
    Digitale Medien
    Digitale Medien
    Correlation between a female sterile mutation and a set of follicle cell proteins in Drosophila melanogaster (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 213-216 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Follicle cell ; Protein ; Female sterile ; Mutation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In order to correlate the synthesis of a previously described set of follicel cell (Fc) proteins with a known mutation that affects female fertility, three female sterile mutations, fs(1)384, fs(1)508 and fs(1)1501, mapping in the same region as the Fc locus (7C1-9), were analysed with respect to Fc synthesis. The fs(1)508 strain displayed a normal Fc protein pattern, while in fs(1)384 no Fc protein synthesis could be detected. The fs(1)1501 pattern of Fc polypeptide synthesis was totally different from that of any previously analysed strain, displaying a set of proteins that were much larger than the standard Fc variant form. Two of the female sterile mutations, fs(1)384 and fs(1)1501, were combined in rans with two wild-type strains displaying two different electrophoretic variant forms of the Fc proteins. The combinations were then analysed for Fc protein synthesis, using the fact that females heterozygous for two of the Fc variant forms display both parental forms. The results indicate that the fs(1)384 mutation is directly involved in the synthesis of the Fc proteins, as the trans heterozygotes only synthesize the Fc form derived from the wild-type parent. We also suggest that the large proteins synthesized by the fs(1)1501 mutant are a defective Fc variant form. The nature of the two mutations is also discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00430429
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  • 96
    Digitale Medien
    Digitale Medien
    Neuropeptides in free-living and parasitic flatworms (Platyhelminthes). An immunocytochemical study (1986)
    Springer
    Hydrobiologia 132 (1986), S. 93-99 
    Details
    ISSN: 1573-5117
    Schlagwort(e): Turbellaria ; Cestoda ; Neuropeptides ; Serotonin ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The nervous systems of the turbellarians Microstomum lineare and Polycelis nigra and of the cestodes Diphyllobothrium dendriticum and Schistocephalus solidus were studied by means of the peroxidase-antiperoxidase (PAP) immunocytochemical method, with the use of antisera to the neuropeptides FMRF-amide, vasotocin, leu-enkephalin, met-enkephalin, neurotensin, somatostatin, and VIP, and to the bioamine serotonin. Anti-FMRF-amide positive perikarya and fibers occurred in all species, while the occurrence of the vertebrate brain-gut peptides and serotonin varied between the species. Anti-somatostatin and anti-VIP gave a negative result. Anti-FMRF-amide and anti-vasotocin positive immunoreactivity was found in the brain and gut of M. lineare, and in the CNS and the peripheral nerve net of the cestodes. We suggest that the brain-gut peptides of free-living flatworms act on the subtegumental region in the cestodes, which lack a gut but absorb their nutrients directly through the tegument.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00046234
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  • 97
    Digitale Medien
    Digitale Medien
    Insect olfactory receptor responses to components of pheromone blends (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 451-467 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Cabbage looper ; Trichoplusia ni ; Lepidoptera ; Noctuidae ; insect attractant ; electrophysiology ; olfactory receptor ; pheromone blend
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Multicomponent pheromone systems are common in many insect species. As our knowledge about the number of different chemical compounds actually involved in a particular communication system increases, so too does the need for an efficient neural mechanism for the encoding of behaviorially relevant odor compounds. Here we consider the electrical activity of olfactory receptor neurons in a subset of the individual pheromone-sensitive sensilla on the antennae of male cabbage looper moths (Trichoplupia ni). Responses to single- and multiple-component stimuli, drawn from seven behaviorally active compounds, were obtained at several different intensities. Some blends elicited electrical responses which were not readily predicted from a knowledge of the receptor neuron's response to individual components.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01020566
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  • 98
    Digitale Medien
    Digitale Medien
    Sex pheromone ofAdoxophyes orana: Additional components and variability in ratio of (Z)-9- and (Z)-11-tetradecenyl acetate (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 763-772 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Summerfruit tortrix ; Adoxophyes orana ; Lepidoptera ; Tortricidae ; sex pheromone ; (Z)-9-tetradecenyl acetate ; (Z)-11-tetradecenyl acetate ; (Z)-9-tetradecen-1-ol ; (Z)-11-tetradecen-1-ol ; individual variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Twelve products related to the sex pheromone main components (Z)-9- and (Z)-11-tetradecenyl acetate (Z9–14∶Ac andZ11–14∶Ac, respectively), were identified in female pheromone gland extracts of the laboratory-reared summerfruit tortrix moth,Adoxophyes orana F.v R. These are the geometric isomers and the alcohols of the main components, (Z)-9-dodecenyl acetate, (Z)-11-hexadecenyl acetate, and saturated acetates of 12–22 carbons. The ratio ofZ9–14∶Ac toZ11–14∶Ac in individuals varied from 3.5∶1 to 11∶1 with an average of 6.2; their total added up to 462 ng/female with an average of 182 ng for 2- to 7-day-old individuals. No qualitative or quantitative differences were observed between laboratory and field insects.Z9–14∶Ac,Z11–14∶Ac and the corresponding alcohols were also found in female effluvia. Addition of either of the two alcohols to a blend of the two acetates augmented trap catch in the field. The same was true for (Z)-9,(E)-12-tetradecadienyl acetate which was not detected in gland extracts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01012108
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  • 99
    Digitale Medien
    Digitale Medien
    Comparative metabolism of [3H]psoralen and [3H]isopsoralen by black swallowtail (Papilio polyxenes Fabr.) caterpillars (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 871-884 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Psoralen ; isopsoralen ; furanocoumarin ; Papilio polyxenes ; Lepidoptera ; Papilionidae ; metabolism ; detoxification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The comparative fate of tritiated preparations of a linear furanocoumarin (psoralen) and an angular furanocoumarin (isopsoralen) was determined in last-instar caterpillars of the black swallowtail butterfly (Papilio polyxenes Fabr.). Oral administration of either furanocoumarin at 5 μg/g is followed by rapid metabolism, primarily through oxidative cleavage of the furan ring, and the metabolites are rapidly excreted. Isopsoralen is, however, metabolized at a somewhat slower rate than is psoralen, and levels of unmetabolized isopsoralen in body tissues of the treated caterpillars are about three-fold higher. These data are compatible with the hypothesis that a reduced detoxification rate accounts at least in part for the susceptibility ofP. polyxenes caterpillars to the deleterious effect of isopsoralens.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01020257
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  • 100
    Digitale Medien
    Digitale Medien
    Flavonoid pigments and wing color inMelanargia galathea (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 49-68 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Melanargia galathea ; Lepidoptera ; Satyridae ; marbled white butterfly ; flavonoids ; wing pigmentation ; flavonoid localization ; scales ; reproductive tissues ; sex differences ; ultraviolet radiation protection ; Oudeman's phenomenon ; sex recognition/mate selection ; chemical defense ; insect-plant interactions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Melanargia galathea L. from four different populations were classified into three categories, white, cream, and yellow, according to the background color of their wings. The flavonoid content of the wing and body tissues of butterflies in each of these categories was spectrophotometrically determined. A direct relationship was found between the wing background color and the flavonoid content of the wings; the greater the flavonoid content, the yellower the wings. Butterflies from the yellow category contained an average of 19.6 μg flavone/mg wing tissue; those from the cream, an average of 14.1 μg/mg tissue; and those from the white an average of 8.3 μg/mg wing tissue. Flavonoids represent on average about 1.9% by weight of the yellow wings; 1.4% of the cream wings; and about 0.8% by weight of the white wings. The most frequently encountered wing background color in each of the populations examined was cream; far fewer individuals had white or yellow wings. Flavonoids were not evenly distributed in the wings ofM. galathea; greater quantities occur on the underside of the hindwings and front margins of the forewings, while the ocelli centers of the underside of the hindwings, and the inner margins on the upperside of the hindwings lack flavonoids. Furthermore, flavonoids were found in the white scales ofM. galathea but not in the black. The flavonoid content of the body ofM. galathea was found to be much lower than that of the wings. When expressed as a percentage of that in the wings, the flavonoid content of the body of both sexes was relatively constant; however, the flavonoid content of the female body represents on average about 59% of that in the wings, compared to about 20% in the male. Both the gross flavonoid content and the flavone concentration per milligram of tissue were found to be greater in female than male butterflies. Since the concentration of flavone per cm2 of wing tissue was the same in both sexes, the greater flavonoid content of female than maleM. galathea has been attributed to the deposition of relatively large quantities in the reproductive tissues of the female. The possible role(s) of flavonoid pigments in wing coloration, in protecting vulnerable tissues from damaging ultraviolet radiation, in mate selection and/or sex recognition, in insect defense against vertebrate predators, and as antimicrobial agents inM. galathea has been discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01045590
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