ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Ultrastructure  (80)
  • DNA  (19)
  • hypertension  (19)
  • Drosophila melanogaster  (17)
  • Springer  (135)
  • Cell Press
  • 1985-1989
  • 1980-1984  (135)
  • 1980  (135)
Collection
Keywords
Publisher
  • Springer  (135)
  • Cell Press
Years
  • 1985-1989
  • 1980-1984  (135)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 2 (1980), S. 79-85 
    ISSN: 1432-0983
    Keywords: Meiosis ; Recombination ; DNA ; Saccharomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In meiotic cells of Saccharomyces cerevisiae, reduction in molecular weights of DNA in alkaline sucrose gradients is observed concomittantly with premeiotic DNA replication and with commitment to recombination. Following the completion of the latter processes, higher molecular weights are obtained. These single-stranded breaks are found in both old and newly synthesised strands. Similar scissions in DNA are also found in a temperature-sensitive mutant (cdc40/cdc40), which does not undergo commitment to recombination at the restrictive temperature, and in vegetative wild type cells that were previously exposed to sporulation medium. The suggestion that these scissions are the physical manifestation of commitment to recombination is therefore rejected.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Chloroplast ; DNA ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The density, molecular weight, and cellular repetition of DNA molecules associated with the β-DNA satellite of the interfertile algae Chlamydomonas eugametos and C. moewusii are reported. The similarities between these values and those for the chloroplast DNA (cpDNA) in the related alga Chlamydomonas reinhardtii indicate that these satellites represent cpDNA. The buoyant densities of C. eugametos and C. moewusii cpDNAs are indistinguishable from one another, as are those of their respective nuclear DNAs. These densities differ slightly from the densities of the homologous components of C. reinhardtii whole cell DNA. All three species differ with respect to additional minor satellite DNAs and low molecular weight DNAs of unknown cellular location. Differences in the Aval and Smal restriction endonuclease fragmentation patterns of C. eugametos and C. moewusii cpDNAs were employed to study the inheritance of cpDNA in an F1 hybrid which had inherited a non-Mendelian streptomycin resistance marker (sr-2) from the C. eugametos mating-type plus (mt +) parent and in two homoplasmic mitotic segregants from a B 1 hybrid (F1 × C. moewusii) which had been initially heteroplasmic for the resistance marker. Although the cpDNA patterns in the F1 hybrid were similar to those of the C. eugametos ml 1 parent, important differences were noted which suggest that recombination between C. eugametos and C. moewusii cpDNA had occurred. Homoplasmic streptomycin resistant and sensitive mitotic segregants recovered from the B1 hybrid product reveal Aval restriction patterns similar to those of the respective resistant and sensitive parents. These data are consistent with the hypothesis that the sr-2 marker is located in cpDNA and that C. eugametos and C. moewusii cpDNA sequences can coexist in the same chloroplast and, at least sometimes, segregate without extensive recombination. The transmission of low molecular weight DNAs characteristic of C. moewusii but of unknown cellular origin shows no direct correlation with the transmission of the sr-2 marker.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 16 (1980), S. 95-110 
    ISSN: 1432-1432
    Keywords: Evolutionary Divergence ; DNA ; Single copy ; Thermal Stability ; S1 Nuclease ; Hydroxyapatite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary New methods have been applied to the determination of single copy DNA sequence differences between the sea urchin speciesStrongylocentrotus purpuratus, S. franciscanus, S. drobachiensis, andLytechinus pictus. The thermal stability of interspecies DNA duplexes was measured in a solvent (2.4 M tetraethylammonium chloride) that suppresses the effect of base composition on melting temperature. The lengths of duplexes were measured after digestion with S1 nuclease and correction made for the effect of length on thermal stability. The degree of base substitution that has occurred in the single copy DNA during sea urchin evolution is significantly larger than indicated by earlier measurements. We estimate that 19% of the nucleotides of the single copy DNA are different in the genomes of the two sea urchin congeners,S. purpuratus, andS. franciscanus, which apparently diverged only 15 to 20 million years ago.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 16 (1980), S. 211-267 
    ISSN: 1432-1432
    Keywords: Nucleic acids ; Proteins ; Natural selection ; Genetics ; Nonrandom molecular divergence ; Nonrandom REH theory ; Evolution ; mRNA ; DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary REH theory is extended by deriving the theoretical equations that permit one to analyze the nonrandom molecular divergence of homologous genes and proteins. The nonrandomicities considered are amino acid and base composition, the frequencies with which each of the four nucleotides is replaced by one of the other three, unequal usage of degenerate codons, distribution of fixed base replacements at the three nucleotide positions within codons, and distributions of fixed base replacements among codons. The latter two distributions turn out to dominate the accuracy of genetic distance estimates. The negative binomial density is used to allow for the unequal mutability of different codon sites, and the implications of its two limiting forms, the Poisson and geometric distributions, are considered. It is shown that the fixation intensity — the average number of base replacements per variable codon - is expressible as the simple product of two factors, the first describing the asymmetry of the distribution of base replacements over the gene and the second defining the ratio of the average probability that a codon will fix a mutation to the probability that it will not. Tables are given relating these features to experimentally observable quantities inα hemoglobin,β hemoglobin, myoglobin, cytochromec, and the parvalbumin group of proteins and to the structure of their corre-sponding genes or mRNAs. The principal results are (1) more accurate methods of estimating parameters of evolutionary interest from experimental gene and protein sequence data, and (2) the fact that change in gene and protein structure has been a much less efficient process than previously believed in the sense of requiring many more base replacements to effect a given structural change than earlier estimation procedures had indicated. This inefficiency is directly traceable to Darwinian selection for the nonrandom gene or protein structures necessary for biological function. The application of these methods is illustrated by detailed consideration of the rabbitα -andβ hemoglobin mRNAs and the proteins for which they code. It is found that these two genes are separated by about 425 fixed base replacements, which is a factor of two greater than earlier estimates. The replacements are distributed over approximately 114 codon sites that were free to accept base mutations during the divergence of these two genes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 188 (1980), S. 65-73 
    ISSN: 1432-041X
    Keywords: Nuclear migration ; Cleavage ; Microtubules ; Ultrastructure ; Gall midge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the eggs ofWachtliella persicariae the cleavage nuclei move relative to the surrounding ooplasm. This ‘active’ migration is caused by an organelle whose ultrastructure was studied throughout the mitotic cycle. It consists of a greatly enlarged polar cytaster derived from the mitotic apparatus, linked to the nucleus by 100 Å filaments. The microtubules of the cytaster were found only during periods of active nuclear migration, i.e., from the onset of anaphase to the early prophase of the next mitotic cycle. They are always solitary and follow the course of the astral rays, which are known to temporarily adhere to peripheral structures of the egg cell and to exert tractive forces. In contrast to the cytaster microtubules, the microtubules in the spindle are bundled and persist from early metaphase through late telophase. During ontogenesis the first migration cytaster is built up between 3 and 12 min after oviposition near the anterior egg pole, in the vicinity of the sperm nucleus. In non-inseminated eggs time lapse films show a migration cytaster to develop autonomously in a region free from nuclei, but it does not follow the normal path of the male pronucleus. In several cases the female pronucleus, which remains without a cytaster of its own, was observed to move to the cytaster generated in the absence of the male pronucleus. Whether or not it is adhering to a nucleus, the cytaster divides into two at the correct time, i.e, corresponding to the first cleavage division in fertilized eggs. In some non-inseminated eggs this type of ‘pseudocleavage’ has been observed to occur repeatedly, giving rise to an increasing number of anucleate cytasters.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 188 (1980), S. 127-132 
    ISSN: 1432-041X
    Keywords: Pole cell isolation ; Maternal effect mutants ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A procedure for pole cell isolation has been developed that takes advantage of theDrosophila melanogaster maternal effect mutantmat(3) 1. Embryos derived from homozygousmat(3)1 mothers form exclusively pole cells. By outcrossing we could substantially increase the expressivity of the original mutant stock. We further introduced theTM8 balancer chromosome, which carries the dominant temperature sensitive mutationDTS-4. This allows the accumulation of large homozygousmat(3) 1 fly populations by eliminating the heterozygous flies at the restrictive temperature. Early embryos were mechanically fragmented and the cells were isolated by means of metrizamide step gradients. The isolated cells were demonstrated to exhibit the various ultrastructural and histochemical characteristics of pole cells. The isolated cells were transplanted into genetically marked host embryos. The germ line mosaics that were obtained indicate that the isolated cells represent functional pole cells. Proteins synthesized by the isolated pole cells during short term in vitro labelling with35S-methionine were compared to the proteins synthesized by blastoderm cells fromOregon-R embryos. At least one protein could be demonstrated in the pole cell samples that is not synthesized byOregon-R blastoderm cells. The method allows a fast and gentle isolation of highly enriched pole cell populations which are a prerequisite for the biochemical analysis of germ cell determination and differentiation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 188 (1980), S. 163-177 
    ISSN: 1432-041X
    Keywords: Yolk sac ; Ultrastructure ; Embryogenesis ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Changes at the ultrastructural level during germ band extension in the embryo ofDrosophila melanogaster are described. Cytoplasmic connections between cells and the yolk sac are present during initial cellular movements. At this time, a continuous system of microfilaments is present adjacent to the membranes in the connections and at the periphery of the yolk sac. As germ band extension progresses, this system becomes discontinuous, and microfilaments are apparent only in the immediate vicinity of the connections. Cytoplasmic connections are disassembled at approximately the midpoint of extension; at the same time, extensive membrane associations develop between germ band cells and between these cells and adjacent yolk sac membranes. Positioning and orientation of cytoplasmic connections suggest that the yolk sac, via these connections, is actively involved in the cellular movements of early germ band extension.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 31 (1980), S. 93-108 
    ISSN: 1432-0827
    Keywords: Calculus ; Ultrastructure ; Apatite ; Transmission ; Scanning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Using transmission and scanning electron microscopy, we have studied the ultrastructure of a number of urinary calculi, mainly composed of calcium phosphate. Three fundamental kinds of calcium phosphates were detected: nonstoichiometric carbonate apatite, nonhexagonal octacalcium phosphate, and calcium-magnesium whitlockite. The influence that the organic matter, substitutions in the phosphate lattice of CO3 and Mg, and apatitic stoichiometry have on the ultrastructure of the calcium phosphate calculi has been detailed. An originating apatitic unity named U2 is assumed to be the responsible for all the different structures of calcium apatites appearing in renal calculi. On the basis of our observations, a mechanism whereby apatites grow is postulated; magnesium functions as an inhibitor for the growing mechanism.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 30 (1980), S. 27-34 
    ISSN: 1432-0827
    Keywords: Ultrastructure ; Calcium ; Cartilage ; Vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The potassium pyroantimonate technique was utilized for the selective subcellular localization of calcium in the mandibular condylar cartilage of 1-day-old rats. Electron dense calcium pyroantimonate precipitates were localized principally in mitochondria and at the cell membrane of the chondrocytes. In addition, small intracellular vesicles 0.1–0.2µm in diameter were observed in proximity to the cell membrane of chondrocytes of the mid-hypertrophic zone. The results suggest that these vesicles were being extruded from the cell into the extracellular matrix. Energy-dispersive analysis by X-rays confirmed that calcium is the principal cation of the electron-dense precipitates.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Planta 149 (1980), S. 69-77 
    ISSN: 1432-2048
    Keywords: Chromatin ; DNA ; Hordeum Nucleus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method for isolation and purification of intact nuclei from barley leaves was developed and several properties of the chromatin were studied. The dense structure of the main part of the chromatin does not alter the accessibility of the DNA to nucleases. 60% of the nuclear DNA can be degraded by micrococcal endonuclease. Nevertheless the solubility of the chromatin fragments depends on the extent of nuclease digestion; solubilisation occurring only when the major part of the internucleosomal DNA was degraded (≃30% of digestion). Electron microscopic observations suggest that this was due to particularly dense organization of the chromatin “in situ”. The possible physiological meaning of some of these properties are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Planta 148 (1980), S. 346-353 
    ISSN: 1432-2048
    Keywords: Chromatin ; DNA ; Germination (embryos) ; Nucleosomes ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Micrococcal nuclease digestion of chromatin from ungerminated and 48 h-germinated pea embryos yields DNA fragments which are multiples of basic units of 194–195 base pairs. Extensive digestion produces a core particle of 145 base pairs. Deoxyribonuclease I gives rise to fragments which are multiples of 10 bases upon analysis on denaturing gels. These values are comparable with those found for other plant materials. These results indicate that gross changes in nucleosomal organization do not accompany the onset of germination.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 12
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 321-326 
    ISSN: 1432-1041
    Keywords: sotalol ; hypertension ; renal impairment ; chronic administration ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Ten hypertensive patients with moderate to severe impairment of renal function were treated with sotalol for 5 to 10 weeks (average 6.4 weeks). Dosage was individually titrated (range 80 to 480 mg daily). The drug was given once daily in the morning. In eight patients blood pressure was satisfactorily controlled. Higher steady-state levels were observed than have been reported after similar doses in patients with normal renal function. The apparent first-order elimination rate constant and plasma clearance were significantly correlated with glomerular filtration rate. For an anuric patient, serum half-life was calculated to be 69 h. In relation to the raised plasma levels, side effects were uncommon. Since sotalol is excreted predominantly via the kidney, therapy in patients with impaired renal function should start with a low dose and any increase in dosage should be made carefully. As the anti-hypertensive effect does not appear to be correlated with the plasma level or with tolerance, adjustment of dose should be based on clinical response.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 13
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 225-229 
    ISSN: 1432-1041
    Keywords: metoprolol ; hypertension ; slow-release preparations ; maximal exercise power ; fatigue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The treatment of hypertension with a single daily-dose of a beta-blocker gives rise to high peak-plasma concentrations 1.5 h after ingestion. After slow release-preparations of beta-blockers, the peak concentrations are half those produced by the conventional preparation at the same oral dose. A frequently occurring side-effect of beta-blocker therapy is fatigue. In this study the effect of a single dose of metoprolol 300 mg, 200 mg, 200 mg slow-release and a placebo on maximal exercise power was tested in 6 healthy subjects, 1.5 h and 24 h after ingestion. Maximal exercise power was significantly reduced 1.5 h after ingestion of metoprolol 300 mg and 200 mg. No change was found 1.5 h after 200 mg of a slow-release preparation. The possible reasons for reduced maximal exercise power are discussed. It is concluded that use of a beta-blocker for the treatment of hypertension in a single daily-dose regimen may be a reason to prefer a slow-release preparation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 14
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 399-401 
    ISSN: 1432-1041
    Keywords: prazosin ; hypertension ; central monoaminergic neurons ; monoamine metabolites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Six hypertensive patients were treated with prazosin up to a final dose of 3–4.5 mg/day. There was a significant reduction of blood pressure. The cerebrospinal fluid (CSF) concentrations of the major metabolites of noradrenaline, dopamine and serotonin were unchanged. This indicates that the antihypertensive effect is not mediated via central monoaminergic neurons as suggested by animal studies.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 15
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 105-108 
    ISSN: 1432-1041
    Keywords: renin ; angiotensin ; aldosterone ; hypertension ; hypoaldosteronism ; pseudohypoaldosteronism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary There is increased activity of the renin, angiotensin, aldosterone (RAA) system in infancy and childhood. An inverse relationship between plasma renin, aldosterone and age has been demonstrated. In childhood hypertension due to renovascular disease or pyelonephritic scarring peripheral plasma renin is increased. Renal vein renin measurements in children with renal hypertension have proved valuable in predicting surgical curability of the underlying lesion. The upper limit of normal for the renal venous renin ratio in normotensive children without renal disease is 1.5. Pharmacological blockade of the RAA system has a place in diagnosis and treatment of hypertensive children. The plasma renin aldosterone profile is diagnostically useful in the investigation of salt-wasting disease and can easily distinguish between aldosterone biosynthetic defects and pseudohypoaldosteronism.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 16
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 135-139 
    ISSN: 1432-1041
    Keywords: hypertension ; labetalol ; propranolol ; renal haemodynamics ; glomerular filtration rate ; blood pressure ; exercise ; renal blood flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The effect of exercise on renal haemodynamics was examined in young patients with mild essential hypertension. Four groups of subjects were studied: 13 normotensive, healthy control subjects, and 15 untreated, 11 propranolol-treated, and 6 labetalol-treated patients. Renal plasma flow (RPF) and glomerular filtration rate (GFR) were measured during four consecutive periods, a pre-exercise control period, two exercise periods with loads of 450 kpm/min and 600 kpm/min, respectively, and a post-exercise control period. In the untreated patients RPF and GFR were lower during exercise than in the normotensive control subjects, whereas no significant differences were found at rest. In the propranolol-treated patients the reduction in RPF and GFR during exercise was more pronounced than in the untreated hypertensives. In the labetalol-treated patients however, RPF and GFR were reduced only to the same degree as in the untreated hypertensives. The reduced renal blood flow in propranolol-treated patients may be attributed to a compensatory increase in sympathetic activity caused by an impaired cardiac response to exercise. The lack of reduction in renal blood flow during labetalol therapy could partly be related to alpha-adrenergic blockade in the renal vascular bed induced by labetalol, and partly to the smaller reduction in cardiac output during labetalol than during propranolol therapy.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 17
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 231-235 
    ISSN: 1432-1041
    Keywords: molsidomine ; hypertension ; hypotension ; angina pectoris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Molsidomine (M), a new long-lasting antianginal compound, was studied in 38 hypertensive patients to assess its antihypertensive properties. Six patients were selected for an acute, single dose comparative trial with placebo over 8 h after treatment. The remaining 32 patients were used in a 1 month trial to study the effect on BP of more prolonged treatment. Systolic, diastolic and mean BP were significantly reduced after a single dose of M 4 mg, and the effect lasted for about 8 h. M also inhibited the hypertensive response to isometric exercise in handgrip tests performed 1 and 8 h after M ingestion. A dose-related decrease in systolic and diastolic BP in the one month trial was also observed. In addition to its antianginal properties, M appears to possess an interesting effect on BP in mildly to moderately hypertensive patients. A fall in BP is also a valuable effect in coronary patients with augmented metabolic demands of the heart.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 18
    ISSN: 1432-1041
    Keywords: hypertension ; nifedipine ; beta-adrenoceptor blockade ; hypotensive action ; adverse effects ; combination therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The antihypertensive effect of nifedipine (10–20 mg t.i.d.) given alone, or in combination with a beta-adrenoceptor blocking drug, was related to the observed plasma concentration during one dosage interval at steady-state (Pl-Nifss). The study was carried out as a within-patient comparison of treatment with nifedipine or placebo for 4 weeks. A highly significant reduction in blood pressure was obtained during monotherapy, as well as during combined treatment. The blood pressure reduction when nifedipine was added to beta-adrenoceptor blockade was of the same magnitude as that observed on nifedipine monotherapy. A considerable variation in Pl-Nifss was noted (range: 2–70 ng/ml). No significant correlation was found between the percentage reduction in blood pressure and Pl-Nifss in either of the two groups. There was a close relationship between Pl-Nifss and the concentration found 4 h after the morning dose. Side-effects were common during nifedipine monotherapy and were the reason for discontinuation of treatment in 4 of 18 patients. In contrast, none of the 9 patients on combined treatment dropped-out. In neither of the treatment groups was there any evidence for sodium retention and volume expansion during the first 4 weeks expressed as weight gain or signs of cardiac insufficiency. However, in 13 patients who continued on long-term treatment for 3–14 months, a definite need for concomitant diuretic therapy was found. The results indicate that nifedipine is effective in lowering blood pressure in patients with mild to moderate essential hypertension, either when given alone or in addition to beta-adrenoceptor blockade. It appears best tolerated as combination therapy. Long-term treatment requires addition of a diuretic. Pl-Nifss did not seem to be a major determinant of the magnitude of the hypotensive response.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 19
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 375-382 
    ISSN: 1432-1041
    Keywords: calcium antagonist ; verapamil ; hypertension ; vasodilators ; plasma renin activity ; mode of action ; sodium balance ; fluid balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary In 12 in-patients with moderate uncomplicated hypertension, maintained on constant sodium intake for 15 days, single-blind oral administration of verapamil 80–160 mg t. i. d. for 10 days had a significant antihypertensive effect: in the supine position systolic blood pressure decreased from 177±5 to 150±3 mmHg, and diastolic pressure from 111±3 to 96±2 mmHg; standing values were similarly lowered from 171±7 to 143±4 mmHg, systolic, and from 118±4 to 97±2 mmHg, diastolic. The heart rate did not show any significant change (from 79±3 to 77±2 beats/min, supine, and from 92±3 to 87±3 beats/min, upright). The antihypertensive effect was uniform throughout the day, being similar 2, 3, 6 and 8 h after administration of a dose. Dynamic exercise (75–100 watts on a cycle-ergometer) caused identical increases in arterial pressure and heart rate on the last day of placebo and again on the last day with verapamil, but the peak levels of systolic pressure reached during exercise were lower after verapamil than with placebo, because of the lower blood pressure before exercise. Reduction of arterial pressure by verapamil was not accompanied by increased plasma renin activity, or by renal retention of sodium and water: there was a small increase in sodium excretion, at least during the first days of verapamil administration (from 107±15 to 113±15 mEq Na+/day), and a slight significant reduction in body weight (from 74.2±3.7 to 73.5±3.7 kg). It is concluded that oral administration of verapamil significantly lowers blood pressure without simultaneously inducing cardiac stimulation, renin secretion or salt and water retention.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 20
    ISSN: 1432-1041
    Keywords: atenolol ; hypertension ; plasma renin activity ; pharmacokinetics ; pharmacodynamic effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary To show whether repeated administration of atenolol for several days would influence its pharmacokinetic parameters and the extent and duration of the pharmacologic responses, the plasma level of atenolol and changes in heart rate, blood pressure and plasma renin activity were measured in 12 hypertensive patients at various times of day (9 a. m., 12 noon, 3 p. m. and 7 p. m.) after oral administration of the first dose of atenolol 100 mg, again during the 7th and 14th days of continued once-daily administration of the same dose, and finally during the three days following withdrawal of the drug. The peak plasma concentration of atenolol (about 600 ng/ml) was found 3 h after administration of the first dose, and measurable amounts (50–70 ng/ml) were found after 24 h. None of the pharmacokinetic characteristics were changed by administration of a single daily dose for two weeks. After withdrawal of the drug, detectable amounts of atenolol were found in plasma for at least 48 h. The first dose of atenolol caused prompt (3 h) and prolonged (up to 24 h) lowering of supine and standing systolic and diastolic blood pressures, slowing of supine and standing heart rate, reduction of the blood pressure and heart rate responses to dynamic exercise, and a decrease in plasma renin activity. The extent and time-course of all these responses were not influenced by repeated once-daily administration of the 100 mg dose for two weeks. Most of the effects continued during the withdrawal days, the lowering of blood pressure being somewhat more prolonged than the slowing of heart rate. It is concluded that a once-daily dose of atenolol 100 mg decreases blood pressure and heart rate throughout the following 24 h, without excessive daily fluctuation in its effects, and without signs of tolerance or accumulation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 21
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 17 (1980), S. 239-241 
    ISSN: 1432-1041
    Keywords: timolol ; hypertension ; dose ranging ; double-blind trial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Nine previously untreated patients with mild-moderate hypertension were included in a dose-ranging double-blind trial to determine the effectiveness of daily versus thrice daily timolol administration. In 8 patients control of blood pressure was equally effective though with significantly lower heart rate achieved in the once daily group. One patient was not satisfactorily controlled on the daily regimen demonstrating that many but not all hypertensives can be controlled with daily administration of timolol.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 22
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 18 (1980), S. 391-394 
    ISSN: 1432-1041
    Keywords: propranolol ; hypertension ; beta-adrenergic blockade ; exercise heart rate ; pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The pharmacokinetics of propranolol in 16 hypertensive patients was compared after the first oral dose of 80 mg and during chronic treatment with 80 mg bd. The degree of beta-adrenergic blockade was estimated by the reduction in maximal exercise heart rate. No significant change in plasma half-life occurred and there was no correlation between the mean steady-state propranolol concentration and beta-adrenergic blockade or antihypertensive effect. A linear relationship was observed between the decrease in blood pressure and the reduction in heart rate during maximal exercise. Therefore, the antihypertensive effect of propranolol can be explained by its peripheral beta-adrenergic blocking properties.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 23
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 17 (1980), S. 329-332 
    ISSN: 1432-1041
    Keywords: reserpine ; hydrochlorothiazide ; pituitary hormones ; gonadal hormones ; hypertension ; serum concentration ; antihypertensive therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Sex histories and serum samples were obtained from 27 hypertensive men before and after 3 months of therapy with either 100 mg of hydrochlorothiazide or 0.25 mg of reserpine daily. Sera were analyzed for testosterone, dihydrotestosterone, estradiol, luteinizing hormone and prolactin. Both drugs effectively lowered blood pressure. The incidence of impaired sexual performance was low and insignificantly different in the two treatment groups. There were no significant changes in serum hormone concentrations as a result of drug therapy.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 24
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 17 (1980), S. 409-413 
    ISSN: 1432-1041
    Keywords: penbutolol ; hypertension ; propranolol ; double-blind crossover comparison ; blood pressure ; heart rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Penbutolol is a potent long-acting non-cardioselective beta-adrenergic blocker with partial agonist activity. A double-blind cross-over comparison of penbutolol given in a single daily dose and propranolol given twice daily in the treatment of ambulant patients with moderate hypertension is described. Fourteen patients completed the study and were treated with each drug for 12 weeks. Penbutolol in daily doses of 20–120 mg and propranolol in daily doses of 80–400 mg produced similar significant reductions in both supine and erect blood pressure. Penbutolol did not reduce heart rate to the same extent as propranolol, in equivalent doses. Penbutolol appears to produce adequate control of moderate hypertension when administered once a day, and this effect appears to be equivalent to divided doses of propranolol. No serious adverse effects were reported, although one patient receiving penbutolol experienced severe eye pains at a dose of 40 mg which resolved on crossing over to treatment with propranolol.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 25
    ISSN: 1432-1041
    Keywords: papaverine ; propranolol ; pindolol ; hypertension ; isoprenaline ; haemodynamic effects ; blood pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The influence of two β-adrenoceptor antagonists, propranolol and pindolol, on the haemodynamic effects of papaverine, isoprenaline and noradrenaline was investigated in 9 male patients with first degree essential hypertension. Propranolol and pindolol were given according to a doubleblind, crossover scheme. Heart rate and blood pressure were measured before and after each treatment. Propranolol 670 µg/kg i. v. reduced the supine and standing systolic blood pressures by 2.3% and 1.6%, respectively. Similarly, the intravenous administration of pindolol 35 µg/kg reduced supine and standing systolic blood pressure by 5.5% and 8.3% respectively (clinically insignificant). Neither drug affected diastolic blood pressure. Following propranolol, there were moderate reductions in supine and standing heart rates, respectively by 24% and 20% (p〈0.001). Similarly, but to a lesser extent, pindolol reduced supine and standing heart rate by 12% and 17% (p〈0.001). The effects of papaverine, which, at 1.5 mg/kg i. v. reduced systolic blood pressure by 5–10% and increased heart rate by 8–15%, were not significantly influenced by the β-blockers. The blood pressure and heart rate responses to isoprenaline, on the other hand, were attenuated or inhibited by both β-blockers. While the β-blockers inhibited the β-adrenoceptor component of noradrenaline, the pressor component of noradrenaline, which is mediated through the α-adrenoceptors, was not influenced by propranolol, but was inhibited after pindolol. It is concluded that pindolol differs qualitatively from propranolol in that it inhibited both the α-and β-adrenoceptor effects of noradrenaline.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 26
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 17 (1980), S. 321-328 
    ISSN: 1432-1041
    Keywords: metoprolol ; hypertension ; pharmacokinetics ; plasma renin ; blood pressure effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary Eleven patients were treated for essential hypertension with metoprolol (Selokén®) for more than three months. The time course of changes in blood pressure, pulse rate and plasma renin activity was studied during treatment with an oral maintenance dose of 100 mg twice daily. Significant decreases in pulse rate, diastolic blood pressure and plasma renin activity were observed even after the first dose. The plasma concentration of metoprolol reached equilibrium after the second dose. After the third dose there was no further significant change in blood pressure. There was a significant correlation (p〈0.001) between the initial (after three doses) and final (after 〉90days) effect of metoprolol on blood pressure (r=0.86 and 0.91 for systolic and diastolic blood pressure change, respectively).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 27
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 17 (1980), S. 161-164 
    ISSN: 1432-1041
    Keywords: nifedipine ; hypertension ; blood pressure ; heart rate ; plasma renin activity ; aldosterone ; clinical trial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The magnitude and duration of the antihypertensive effect of nifedipine were studied in 7 cases of moderate essential hypertension. In a double-blind crossover study, nifedipine 10 mg or a placebo were administered sublingually 4 times a day for 2 days, and the results were compared. Each dose of nifedipine reduced systolic and diastolic blood pressure by 14% both in the supine and upright positions. The antihypertensive action lasted for about 3 h and it was not cumulative. The reduction in blood pressure was associated with a temporary increase in heart rate. Administration of nifedipine 10 mg did not significantly raise plasma renin activity or plasma aldosterone. The drug was well tolerated and no side effects were detected.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 28
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical pharmacology 17 (1980), S. 333-337 
    ISSN: 1432-1041
    Keywords: atenolol ; chlorthalidone ; hypertension ; chronic treatment ; co-administration ; plasma half-life ; urine half-life
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The pharmacokinetics of atenolol with and without the co-administration of chlorthalidone were studied in five hypertensive subjects. Concomitant administration of chlorthalidone appears to have little if any effect on the pharmacokinetics of atenolol during treatment for 7 days. The atenolol elimination half-lives were 6.7±1.1 and 6.3±0.9 h, respectively, with and without chlorthalidone. Two healthy volunteers also received a single 50 mg oral dose of chlorthalidone. Their blood profiles and pharmacokinetics were similar to those observed in hypertensive subjects, but a statistically significant difference (p〈0.01) was found between the urinary excretion half-lives of chlorthalidone. This difference may be because chronic administration of the drug caused saturation of red cell binding.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 29
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 128 (1980), S. 113-119 
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; DNA ; Synchrony ; 2′-Deoxyadenosine ; S period ; Duplication cycle ; Cell cycle ; Conidia ; Urografin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 2′-Deoxyadenosine (2 mM), a DNA inhibitor, was used to synchronize DNA synthesis in cultures of Neurospora crassa lys 3. The cultures recovered spontaneously from the inhibitor which had little or no effect on the synthesis of RNA, protein or carbohydrate or on the specific growth rate of the mould. The degree of ‘synchrony’ of DNA synthesis obtained with 2′-deoxyadenosine varied directly with the organism's specific growth rate when the latter was altered by temperature changes. A direct relationship was observed between the rate of synthesis of DNA during the S period and the organism's specific growth rate. Conidia of Neurospora crassa lys 3 were separated into different density classes using urografin gradients; the separation treatment did not have an appreciable effect on the subsequent germination or growth of conidia. Populations of large, less dense conidia produced germ tubes more rapidly and more synchronously than populations of small, dense conidia. Cultures inoculated with the large conidia displayed continuous synthesis of RNA and protein but discontinuous synthesis of DNA.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 30
    ISSN: 1432-072X
    Keywords: Mating tube ; Microtubule ; Tremella ; Ultrastructure ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ultrastructure of the mating tube formed in yeast haplont of the heterobasidiomycete Tremella mesenterica was studied by electron microscopy. Cell wall of the mating tube emerged as evagination of the inner layers, rupturing outer layers of the mother cell wall. Comparison with budding cells suggested that the tube emergence place at bud scar and the process of tube emergence was the same as that of bud emergence. Electron transparent vesicles of 0.1 μm diameter were scattered in the cytoplasm of the mating tube. Nucleus-associated organelle was located at one side of the nuclear envelope which extended towards the mating tube. A few microtubules were detected in the mating tube, but their association with a nucleus was not clear. The cytoplasmic structure of the mating tube was discussed in comparison with that of hyphae of the filamentous fungi.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 31
    ISSN: 1432-072X
    Keywords: Claviceps purpurea ; Ultrastructure ; Development ; Sclerotium ; Oleosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of sclerotia of Claviceps purpurea was investigated by light and electron microscopy. During the first days after infection sterigma and conidiospores are formed. The spores show a moderately developed vacuolar system, they are thick walled and contain about 20% lipid (related to the cell volume) embedded in glycogen. The sterigma are cylindrical unicellular hyphae with electron dense cytoplasm and isolated strongly contrasted lipid droplets. In maturing sclerotia the hyphae become septated with increasingly thick cell walls and a large lipid content. The lipid forms small droplets in young cells, while in the mature sclerotium it occurs in the form of very large drops, occupying the major part of the cell. Simultaneously the composition of the lipid is changed. The mature cells have several nuclei. They are partially connected by osmiophilic substances, forming a network of intercellular spaces.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 32
    ISSN: 1432-072X
    Keywords: Anacystis nidulans ; Cyanobacterium ; Chemostat culture ; RNA ; DNA ; Protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The DNA, RNA and protein content of the cyanobacterium Anacystis nidulans was determined in light-limited and carbon dioxide-limited chemostat cultures over the dilution rate range, D=0.02 h-1 to 0.19 h-1. The macromolecular contents as a percentage of the dry weight and on a per cell basis varied significantly as a function of organism growth rate and the nature of the growth conditions. For both limitations the RNA content per cell increased [20–55 fg RNA (cell)-1] with increasing dilution rate and also showed an increase as a percentage of the dry weight. The DNA content as a percentage of the dry weight showed a 2-fold decrease with increasing dilution rate over the range examined. On a per cell basis DNA reached a peak at D=0.1 h-1 [4.5 fg DNA (cell)-1] for light-limited organisms and at D=0.08 h-1 [8.0 fg DNA (cell)-1] for carbon dioxide-limited organisms. The q RNA increased with increasing dilution rates over the complete growth rate range examined whilst q DNA reached a maximum at D=0.09 to 0.10 h-1. The protein content as a percentage of the dry weight was greater in CO2-limited organisms than light-limited organisms but in both cultures declined as the dilution rate was increased above D=0.10 h-1.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 33
    ISSN: 1573-3297
    Keywords: Drosophila melanogaster ; choice of oviposition site ; strain difference ; site discrimination ; egg insertion ; selection ; wild type
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Females ofDrosophila melanogaster were given a choice of oviposition site either on the surface of the medium or on the surface of paper positioned vertically on the medium. A significant difference was seen in the proportion of eggs deposited on the paper among wild strains of different geographic origins. Bidirectional selection for oviposition on these two sites was effective. These selected lines were examined under various conditions to determine the factors involved in this site selection for oviposition. The lines that chose medium laid eggs only on substrates into which egges could be inserted. The lines preferring paper showed no strict requirement for burying their eggs. Tarsal sensillae were involved in site discrimination.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 34
    Electronic Resource
    Electronic Resource
    Springer
    Behavior genetics 10 (1980), S. 163-170 
    ISSN: 1573-3297
    Keywords: Drosophila melanogaster ; mating success ; male fertility ; male age ; female choice experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Female choice experiments were used to investigate the effect of relative male age on mating success inD. melanogaster. Experiments were conducted with a Canton-S (CS) strain, in which two virgin males of different ages (2, 4, or 8 days old) were offered to virgin females. Older males were found to be more successful under competitive conditions. In another group of experiments, vermilion (v) males of different ages competed with CS males of different ages. The competitive success ofv males was found to increase with their relative age. Male fertility at 2, 4, and 8 days of age was documented for both male genotypes mated with CS females. CS males fathered more offspring per copulation thanv males, and the fertility of all males was found to increase with age. Discussion focuses on the changes in male mating success and fertility with age and genotype.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 35
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 18 (1980), S. 65-76 
    ISSN: 1573-4927
    Keywords: Drosophila melanogaster ; cuticle ; chitin ; β-alanine ; N-acetyldopamine ; tanning ; melanization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In Drosophila melanogaster, the chitinous microfibrils arising from the tips of the epidermal villi in adult cuticles remain irregular and loose in the mutant ebony (which fails in cuticular incorporation of β-alanine) but closely knit and regular in normal flies. Addition of β-alanine to cuticles from which nonchitinous materials have been removed with alkali converts the loose arrangement of the microfibrils to a compact and sharply delineated arrangement. β-alanine also accelerates tyrosinase-catalyzed oxidation of N-acetyldopamine by reacting with the oxidized product of the reaction to produce an orange-red complex. Similarly, β-alanine accelerates oxidation of N-acetyldopamine when these two substances are added to fluids from the hemocoel, to lead to tanning instead of normal blackening. These findings may help explain why β-alanine induces tanning while inhibiting melanization in insects.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 36
    ISSN: 1573-4927
    Keywords: Drosophila melanogaster ; 6-phosphogluconate dehydrogenase ; isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract 6-Phosphogluconate dehyrogenase is evident at all developmental stages of Drosophila melanogaster. The activity level is highest in early third instar larvae and declines to a lower, but relatively constant, level at all later stages of development. The enzyme is localized in the cytosolic portion of the cell. The A-isozymic form of 6-phosphogluconate dehydrogenase was purified to homogeneity and has a molecular weight of 105,000. The enzyme is a dimer consisting of subunits with molecular weights of 55,000 and 53,000. For the oxidative decarboxylation of 6-phosphogluconate the Km for substrate is 81 µm while that for NADP+ is 22.3 µm. The optimum pH for activity is 7.8 while the optimum temperature is 37 C.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 37
    ISSN: 1573-4927
    Keywords: Tryptophan ; kynurenine ; white ; Drosophila melanogaster ; amino acid transport ; Malpighian tubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Dissected Malpighian tubules from wild type and the eye color mutant white of Drosophila were compared with respect to their abilities to transport tryptophan and kynurenine into tubule cells. It was determined that mutation at white greatly impairs the ability of Malpighian tubule cells to take up tryptophan. Functional studies on the extracellular spaces and ultrastructural observations indicated no differences in these respects between wild type and white tubules. It is consistent with several observations that much of the tryptophan associated with white exists in the intercellular spaces. Furthermore, the uptake of tryptophan by the w + system of wild type tubules is inhibited by the analogue 5-methyl-tryptophan. However, the incorporation of radioactive tryptophan into protein in tubule cells from wild type and white occurs at the same rates and is not affected by 5-methyl-tryptophan. Therefore, it is apparent that Malpighian tubules have a transport system that enables entry of tryptophan into a cellular pool and that this cellular pool is initially independent of the tryptophan pool used for protein synthesis. The mutant white lacks this transport system. From these studies and others it appears that compartmentalization of cellular pools may be brought about via the utilization of specific membrane transport systems.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 38
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 18 (1980), S. 303-309 
    ISSN: 1573-4927
    Keywords: Drosophila melanogaster ; allozymes ; GPT ; genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We have used electrophoretic variants of glutamate-pyruvate transaminase (GPT, E.C. 2.6.1.2) in Drosophila melanogaster to genetically map the structural gene to position 42.6 on the X chromosome. By pseudodominance tests over several deficiencies we have localized it cytogenetically to the interval 11Fl-2 to 12Al-2. The sedimentation constant (s 20,w) of the native enzyme was determined in sucrose density gradients to be 5.9 and the native molecular weight approximately 87,000. The similarity in physical properties to mammalian enzymes suggests that the enzyme may also be dimeric in D. melanogaster.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 39
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 18 (1980), S. 699-715 
    ISSN: 1573-4927
    Keywords: modifying genes ; G6PD activity ; 6PGD activity ; Drosophila melanogaster ; enzyme polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Different homozygous lines of similar genotype with respect to G6pd and 6Pgd were shown to have different enzyme activities for G6PD and 6PGD. Crosses between high and low lines suggested that there were modifying genes present on the autosomes, while others were probably located on the X chromosome. Allelic variation within each electrophoretic class of G6pd and 6Pgd might, however, also have contributed to this variation. An experiment on adaptation to sodium octanoate demonstrated that in adapted flies selection for lower enzyme activity had occurred, which provided further evidence for the existence of genetic differences in activity. Furthermore, a strong positive correlation between the activities of G6PD and 6PGD was found for each genotype. Since no correlation was found between MDH and the two enzymes G6PD and 6PGD, it could be concluded that this correlation was probably rather specific for G6PD and 6PGD. Interaction between genotypes with respect to activity was also found. It was shown that the variation at 6Pgd influenced the activity of G6PD within a genotype. The data are discussed in relation to fitness differences presented in foregoing articles.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 40
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 18 (1980), S. 743-753 
    ISSN: 1573-4927
    Keywords: RNase-senitive DNA polymerase activity ; RNA ; DNA ; Neurospora crassa ; differential DNA polymerase activity ; cell fractions and mutants of N. crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract RNase-sensitive DNA polymerase activity (RSDP) was tested in different cell fractions of Neurospora crassa cell types and its morphological mutants. This RSDP was found localized in the microsomal pellet fraction and absent in the purified nuclear pellets isolated from different N. crassa cell types: conidia, germinated conidia, and mycelia. This enzyme is capable of synthesizing a DNA product only in the presence of all four deoxyribonucleoside-5′-triphosphates and Mg2+. Removal of RNA from the pellet fraction by RNase strongly inhibited the DNA synthesis. The endogenous synthesis of DNA in the microsomal pellet fraction was associated with the formation of an RNA:DNA hybrid as analyzed by Cs2SO4 equilibrium density gradient centrifugation. The DNA product after alkali hydrolysis hybridizes with the RNA isolated from the same pellet fraction, as analyzed by elution from hydroxylapatite column at 60 C. This DNA product did not hybridize with poly(A). A few mutants tested showed this RNase-sensitive DNA polymerase activity.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 41
    Electronic Resource
    Electronic Resource
    Springer
    Biochemical genetics 18 (1980), S. 905-913 
    ISSN: 1573-4927
    Keywords: Drosophila melanogaster ; allozymes ; α-glycerophosphate dehydrogenase ; frequency-dependent selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Polymorphism at the α-Gpdh locus was studied in Drosophila melanogaster. Using two different lines, one marked by the F allele (FF line) another by the S allele (SS line), four populations were initiated, two in which the initial frequency of F was 0.1 and two in which it was 0.9. They have been observed for 34 generations. From the fifth generation on, the equilibrium frequency in the four cages was about 0.60. Viability has been measured during the evolution of the populations while F frequencies changed and recombinations between the FF and SS lines occurred. It has also been evaluated in synthetic populations built with different frequencies: (1) from the original FF and SS lines and (2) from FF and SS lines extracted after 34 generations of joint evolution. In all three cases, the FF viability depended on the frequency of the F allele. The similarity of the three linear regressions implies that the α-Gpdh locus or other closely linked loci is the target of the selection in the populations analyzed here.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 42
    Electronic Resource
    Electronic Resource
    Springer
    Behavior genetics 10 (1980), S. 183-190 
    ISSN: 1573-3297
    Keywords: ethanol ; Drosophila melanogaster ; larvae ; strain difference ; habitat selection ; isofemale strains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract There is a latitudinal cline in attraction to ethanol of newly hatchedD. melanogaster larvae; attraction decreases as one moves from the temperate regions to the Australian tropics. Tropical populations manifest high levels of heterogeneity compared with temperate regions, making tropical populations less dependent on ethanol. Since ethanol is a resource forD. melanogaster, an approach to the genetics of resource utilization in natural populations via behavior genetics of larval ethanol responses is feasible, using isofemale strains as experimental material.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 43
    ISSN: 1573-3297
    Keywords: Drosophila melanogaster ; Drosophila simulans ; hybrid behavior ; transition analysis ; courtship sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Several transitions between sequential male courtship elements were analyzed forDrosophila melanogaster its close relativeD. simulans, and two types of hybrid males. Hybrid males from special reciprocal crosses did not differ. WhileD. melanogaster andD. simulans males differed markedly for the majority of transitions studied, hybrid males showed no consistent pattern with the parent species, being indistinguishable fromD. simulans males, indistinguishable fromD. melanogaster, or intermediate between them, depending on the trait observed. This suggests independent genetic control of these transitions during male courtship.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 44
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 57 (1980), S. 97-100 
    ISSN: 1432-2242
    Keywords: Zea mays ; Male-sterility ; ‘S’-Cytoplasm ; Mitochondria ; DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple, rapid, and reproducible assay is described for determining unambiguously the presence of S-type cytoplasm in male-sterile and male-fertile (restored) maize lines. Because the assay requires only 0.5 g leaf segment per sample, it is a single plant assay and the plant is not destroyed. Plants at any developmental stage can be used. The assay involves a 30 sec homogenization, 20 min centrifugation, one hour lysis, overnight agarose electrophoresis, 30 min gel staining, and photography of the gel to produce a result in much less than 24 hr. Many samples can be assayed simultaneously. The various assay methods available for classifying maize cytoplasms are compared and discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 45
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 56 (1980), S. 161-173 
    ISSN: 1432-2242
    Keywords: Drosophila melanogaster ; Chromosome ; Spontaneous interchange ; half-translocation ; Non-homologous pairing ; Mitosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Herein is described an attempts to establish chromosome pairing-interchange relationships in Drosophila melanogaster female. For this purpose, the formation of half-translocations was studied in XXY and XX females bearing compounds of the second pair of autosomes. With respect to XXY females, it was expected that the free Y chromosome would pair with these compounds and that half-translocations involving 2L would arise. In as much as compound chromosomes in XX females had no partner for pairing, the formation of half-translocations involving 2L was not expected. Half-translocations were registered in the F1 from crosses of XX and XXY females to b j pr cn/T(Y;2)C males. The cross was designed to permit the detection of very rarely occurring non-homologue interchanges. Offspring number was 335 in XX females and 550 in XXY females. The majority of offspring consisted of individuals arisen from the spontaneous restitution of compounds and the formation of 2n egg cells. Based on phenotype, the offspring of XX females contained 4 individuals with half-translocations involving 2L; there were 48 such flies among the offspring of XXY females. As confirmed by progeny analysis, 38 half-translocations occurred in XXY females and none in XX females. Of the 31 spontaneous interchanges in XXY females 28 were recorded between the Y and the left compound, one between the Y and the right compound, and one between the X and the left compound. Non-homologue interchanges were of oogonial origin judging by the fact that individuals with half-translocations arose in clusters. Unlike Y — left compound interchanges, the interchanges between autosomal compounds seem to be of meiotic origin.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 46
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 57 (1980), S. 25-32 
    ISSN: 1432-2242
    Keywords: Long-term selection ; Residual genetic variability ; Heritability ; Abdominal bristle number ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six replicate lines of Drosophila melanogaster, which had been selected for increased abdominal bristle number for more than 85 generations, were assayed by hierarchical analysis of variance and offspring on parent regression immediately after selection ceased, and by single-generation realised heritability after more than 25 generations of subsequent relaxed selection. Half-sib estimates of heritability in 5 lines were as high as in the base population and much higher than observed genetic gains would suggest, excluding lack of sufficient additive genetic variance as a cause of ineffective selection in these lines. Also, there was considerable diversity among the six lines in composition of phenotypic variability: in addition to differences in the additive genetic component, one or more of the components due to dominance, epistasis, sex-linkage or genotype-environment interaction appeared to be important in different lines. Even after relaxed selection, single-generation realised heritabilities in four lines were as high as in the base population. As a large proportion of total genetic gain must have been made by fixation of favourable alleles, the compensatory increase of genetic variability has been sought in a genetic model involving genes at low initial frequencies, enhancement of gene effects during selection and/or new mutations.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 47
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 57 (1980), S. 113-117 
    ISSN: 1432-2242
    Keywords: Long-term selection ; Relaxed selection ; Reverse selection ; Dominance of bristle number genes ; Drosophila melanogaster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reverse and relaxed selection were carried out in sublines which were derived from six replicate lines of Drosophila during 86–89 generations of selection for increased abdominal bristle number, and the reverse selection sublines were reciprocally crossed with selection lines of their origin. The results of serial relaxed selection initiated at different generations of selection confirm that the accelerated responses observed in the selection lines were largely due to deleterious genes, particularly lethals, with large effects on the selected character. The decline in mean bristle number under relaxed selection was not much different between crowded and uncrowded relaxed sublines. Reverse selection initiated at generation 57 was very effective, though it failed to bring the mean back to the base population level, and the genetic differences between replicate sublines (two from each of the six lines) indicate that low bristle number genes were probably rare in the selection lines. The genes which were still segregating after 57 generations of selection, on the average, did not show any directional dominance. The contribution of the X-chromosome to selection response was proportional to its chromosome length.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 48
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 57 (1980), S. 247-255 
    ISSN: 1432-2242
    Keywords: Compound ; free-arm strains ; Drosophila melanogaster ; Unstable genetic isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crosses between compound-2L; free-2R (free-arm) and standard strains of Drosophila melanogaster produce two classes of inviable aneuploid hybrids in equal proportions: monosomic 2L and trisomic 2L. The lethal period for monosomics occurs during embryogenesis while the trisomics survive to late pupae. Since the hybrids are inviable, standard and free-arm strains within a mixed population remain genetically isolated. Genetic isolation in the absence of mating isolation offers an extreme example of unstable equilibrium. Relative fitness data indicate that an unstable equilibrium will be established between free-arm and standard strains at a ratio of 2.5∶1. Indeed, in three cage experiments established at initial ratios of 3∶1, free arms to standards, laboratory (Oregon R) or native (Okanagan S) standard strains were completely replaced in approximately 100 days by free-arm lines derived either from laboratory or from native genetic background. In contrast, one cage established at an initial ratio of 4∶1 failed to show replacement and for 92 days remained at approximately the initial ratio. Subsequent genetic analysis of flies removed from this cage identified the presence of an anomalous strain through which genetic information was transferred reciprocally between the free-arm and standard lines. The second chromosomes carried by this strain consisted of a free-2R and a standard second on the right arm of which was attached a duplication for all of 2L. While the origin of the 2L·2R+2L chromosome was uncertain, genetic and cytological examinations revealed that it represented the reciprocal crossover product expected from an exchange that generated a F(2R). Additional crosses disclosed that the transmission frequency of the asymmetrical pair of second chromosomes, as well as their right-arm crossover products, was disproportionately in favor of the short arm. Since unequal transmission was invariably greater from female parents, this phenomenon was viewed as further evidence in support of the drag hypothesis.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 49
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 206 (1980), S. 95-114 
    ISSN: 1432-0878
    Keywords: Lateral eyes ; Scorpion ; Ultrastructure ; Retina ; Arhabdomeric cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dioptric apparatus of the lateral eyes of the scorpion, Androctonus austrails, consists of a cuticular lens, but lacks a vitreous body. The retina is formed by (1) retinula cells displaying a contiguous network of rhabdoms; (2) arhabdomeric cells bearing a distal dendrite that contacts retinula cells via numerous projections and ends before the rhabdomere of the retinula cells; (3) pigment cells that ensheath retinula and arhabdomeric cells with the exception of the contact regions; and (4) neurosecretory fibres possibly originating in the supraesophageal ganglion. The ratio of the number of retinula to arhabdomeric cells is determined to be close to 2 ∶ 1 in the three larger anterolateral eyes, in contrast to the median eyes where the ratio is 5 ∶ 1. The construction of the dioptric apparatus as well as the anatomy of the retina imply that in the lateral eyes of Androctonus australis visual acuity is reduced. A certain degree of spatial discrimination, however, may be retained by the presence of a relatively high number of arhabdomeric cells. It is suggested that the lateral eyes of A. australis mainly function as light detectors, e.g., for Zeitgeber stimuli.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 50
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 206 (1980), S. 123-138 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Sense organs ; Compound eyes ; Interfacetal mechanoreceptor ; Coleoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of the compound eyes of adult Cicindela tranquebarica Herbst was examined by use of light, scanning, and transmission electron microscopy. Each ommatidium of these photopic eyes is eucone and has a “subcorneal layer” situated between the corneal lens and crystalline cone. A distal rhabdomere consisting only of microvilli from retinula cell seven, a more proximal, rectangular, fused rhabdom formed from six retinula cells, and a basal, eighth retinula cell with a spherical rhabdomere comprise the light sensitive portions of the ommatidium. The “subcorneal layer” consists of lamellae of endocuticular microfibrils and, in surface view, shows 11 concave polygons. Proximal extensions of the crystalline thread form inter-retinular fibres containing microtubules between retinula cells 1/2, 3/4, 5/6, and 7/1. The primary pigment cells are devoid of pigment granules, but are rich in rough endoplasmic reticulum. Proximal to each retinula cell nucleus are two basal bodies, one perpendicular to the other. The more proximal basal body extends two fibrillar feet proximally which fuse to form a horizontally-banded ciliary rootlet extending the length of the retinula peripheral to the rhabdom. Each ommatidium is surrounded by 16 secondary pigment cells. Interfacetal mechanoreceptors between some adjacent lenses each have a single bipolar neuron, with a dendritic sheath, tubular body, cilium, outer and inner sheath cells, and an axon surrounded by a neurilemma sheath cell.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 51
    ISSN: 1432-0878
    Keywords: Somatostatin cells ; Calcitonin cells ; Ontogeny ; Ultrastructure ; Thyroid (rat)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Calcitonin cells are relatively numerous in the thyroid gland of the rat. In contrast, somatostatin cells are very scarce except at the time of birth and a few days thereafter, when they are conspicuously numerous. Somatostatin cells of the thyroid gland, which are ultrastructurally similar to somatostatin cells in gut and pancreas, also contain immunoreactive calcitonin. It is not clear whether somatostatin cells in the rat thyroid gland produce calcitonin or accumulate calcitonin from the environment.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 52
    ISSN: 1432-0878
    Keywords: Preputial glands ; Nude mice ; Ultrastructure ; Gas chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The preputial glands of male nude (nu/nu) mice were analyzed by a combination of electron microscopy and gas chromatography to determine whether or not they are affected, like developing hairs and nails, by the nu/nu genotype. Results of the analyses revealed no differences between the glands of nude and normal male mice in either their ultrastructural characteristics or lipid secretory products.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 53
    ISSN: 1432-0878
    Keywords: Rat ; Preovulatory follicle ; Ultrastructure ; Degeneration ; Atresia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To identify and describe ovarian follicles committed to undergo follicular degeneration (atresia), immature rats were primed with pregnant mare serum gonadotropin (PMSG). After PMSG treatment, preovulatory follicles develop but subsequently degenerate. Prior to the appearance of pyknotic nuclei (Stage I of atresia), degenerative changes were observed in focal areas of the granulosa cell layer. These changes include “blebbing” of the cytoplasm and alterations in the shape of the granulosa cells. The appearance of these degenerative changes coincides with a decrease in ovarian concentrations of estradiol and testosterone. Since estrogens and androgens maintain the follicle, the decline in estradiol and testosterone could be responsible for the further degenerative alterations that lead to complete deterioration of the preovulatory follicle. In Stage I atretic follicles, lysosome-derived autophagic vacuoles develop and macrophages invade both the thecal and granulosa cell layers. The combined actions of the autophagic vacuoles and macrophages could destroy both the granulosa-cell and thecal layers and thereby transform the preovulatory follicle into an ovarian cyst.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 54
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 212 (1980), S. 241-255 
    ISSN: 1432-0878
    Keywords: Glomerulogenesis ; Glomerular capillaries ; Kidney development ; Corrosion casts ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Kidneys of 2 to 10 day-old rats of Wistar and Sprague-Dawley strains were fixed with glutaraldehyde by retrograde vascular perfusion and then prepared for observation in TEM and SEM. In addition methacrylate casts of differentiating glomerular capillaries were examined by SEM. Although the glomerular vascular pattern differs from one glomerulus to another, its differentiation proceeds according to the following general plan. First the glomerular capillary splits longitudinally, finally to form 3 to 5 lobules consisting of a capillary network, sustained centrally by the mesangium. In the present study the differentiation of glomerular capillaries is described in five successive arbitrarily selected stages. At Stage I a capillary loop penetrates between the lower limb and the middle segment of the S-shaped body, the rudimentary nephron. At Stage II the capillary undergoes a first subdivision, establishing the primitive lobulation of the glomerulus. At Stage III the vascular and urinary poles differentiate. At Stage IV the glomerulus assumes the aspect of a spherical body, and the capillaries in each lobule undergo subdivision. In Stage V the glomerular vascular pattern approaches its adult appearance, although the maturation processes continue for an extended period of time. Hence in the 10 day-old rat the best-differentiated glomeruli are half the size of adult glomeruli, and their capillary loops are proportionally less well-developed. The capillaries of adjacent lobules may communicate with each other, but a direct vascular shunt between the afferent and efferent vessels cannot be demonstrated.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 55
    ISSN: 1432-0878
    Keywords: Stannius bodies ; Secretory cell types ; Teleosts (Fundulus, Carassius) ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Stannius bodies of killifish and goldfish were ultrastructurally investigated after exposure of the fish to media of different osmolality and calcium content. In both species two structurally different secretory cell types are found. Type-1 cells predominate. They contain an extensive granular endoplasmic reticulum, some large Golgi areas, and secretory granules with a mean diameter of about 0.4 μm. These cells are activated by transfer of freshwater fish to seawater, but not by transfer to low-calcium seawater. Type-2 cells often contain cytoplasmic processes that penetrate between the type-1 cells and are ending on the basal lamina. In this contact area granule release takes place. Type-2 cells contain some strands of granular endoplasmic reticulum, several small Golgi areas, and secretory granules with a mean diameter between 0.15 and 0.20 μm. Type-2 cells are not activated after transfer of fish to seawater. In killifish seawater exposure leads to a reduction of type-2 cells. The differences between the reactions of both cell types to different media point to functional differences between their secretory products. Type-1 cells may produce a hypocalcemic factor. It is concluded that type-2 cells are typical for freshwater fish or euryhaline fish spending part of their life cycle in freshwater. The proper function of these cells is unclear.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 56
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 212 (1980), S. 307-314 
    ISSN: 1432-0878
    Keywords: Thymus (Mammalia) ; Erythropoiesis ; Macrocytes ; Anaemia ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The thymus of wild young and adult bank voles (Clethrionomys glareolus) was examined by histological methods for the presence of developing erythroid cells. Nucleated erythroid cells were observed in 26% of the glands examined by light microscopy and in 69% of the glands examined by electron microscopy. The largest number of developing erythroid cells was observed in the thymus of pregnant females, also showing raised reticulocyte counts (3.1–10.2%). However, erythropoiesis could also be found in breeding and non-breeding, first year and older animals. Erythroid cells were mainly located in the cortex, sometimes in small groups interspersed between lymphoid cells, and also randomly scattered in the cortex. Occasionally, macrocytic erythroid cells were also present. Pyknotic cells were commonly present, and granulopoiesis was frequently observed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 57
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 208 (1980), S. 123-133 
    ISSN: 1432-0878
    Keywords: Lesioned axons ; Ultrastructure ; Crayfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distal stumps of severed medial giant axons (MGAs) and of non-giant axons (NGAs) in the CNS of the crayfish Procambarus clarkii show long-term (5–9 months) survival associated with disorientation of mitochondria and thickening of the glial sheath. However, the morphological responses of the two axonal types differ in that neither the proximal nor the distal stump of severed MGAs ever fills with mitochondria as is observed in some severed NGAs. Furthermore, the adaxonal glial layer never completely encircles portions of MGA axoplasm as occurs in many severed NGAs; in fact, ultrastructural changes in the adaxonal layer around severed MGAs are often difficult to detect. No multiple axonal profiles are ever seen within the glial sheath of the proximal or distal stumps of severed MGAs whereas these structures are easily located within severed NGAs.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 58
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 211 (1980), S. 223-234 
    ISSN: 1432-0878
    Keywords: Neurosecretion, insect ; Median neurosecretory cells ; Melanogryllus desertus ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Based on the nature of their granules, eight principal types of neurons, six of which are thought to be neurosecretory, are recognized in the median neurosecretory cell group of the brain of Melanogryllus desertus. Most of the neurosecretory cells contain granules with diameters of 200–300 nm. In a few the granules are smaller with diameters varying from 60–100 nm. Most of the cells have well developed Golgi areas and dense bodies of different sizes. Dense bodies are closely associated with neurosecretory granules. Accumulations of electron-dense granular material occur in expanded cisterns of endoplasmic reticulum, particularly in type-I cells.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 59
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Fluorescence histochemistry ; Prevertebral ganglia ; Ageing ; 5-hydroxydopamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sympathetic post-ganglionic neurones in the coeliac-superior mesenteric ganglion (CSMG) complex of aged (24 month) rats have been studied by glyoxylic acid-induced fluorescence and electron microscopy. Comparisons have been made with the CSMG of young adult (4 month) rats. In the aged rats the noradrenaline fluorescence of the majority of neuronal perikarya was very low or absent and few intraganglionic fluorescent varicosities were seen. Lipofuscin pigment was very prominent at the nuclear pole region of neurones and also in dendrites and axonal processes. Ultrastructural studies revealed large accumulations of residual bodies at the nuclear poles and in axons and dendritic profiles. Within the perikarya many mitochondria were distorted or swollen, the rough endoplasmic reticulum was disarranged and much dilated as were Golgi cisternae. Primary lysosomes were encountered throughout the neurone perikaryon and its axonal or dendritic processes. In contrast to the young adult CSMG, no evidence for loading of transmitter storage vesicles with an identical dose level of 5-hydroxydopamine was detected in any part of the neurones of aged rats. This might reflect an impairment of the uptake mechanisms and/or storage of noradrenaline in aged sympathetic neurones and their axonal and dendritic processes.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 60
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 205 (1980), S. 273-287 
    ISSN: 1432-0878
    Keywords: Human neurohypophysis ; Ultrastructure ; Pituicytes ; Ependyma ; Choristoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pars nervosa of the neurohypophysis from 12 patients undergoing hypophysectomy for palliative treatment of advanced carcinoma was studied electron microscopically. Special attention was given to the cellular elements, the pituicytes. Five different classes of pituicytes, with various transitional forms, were elucidated based on their ultrastructural characteristics: (1) The most common type, referred to as “major pituicytes”, is reminiscent of astrocytes. (2) “Dark pituicytes” are thought to represent different functional stages of the “major pituicytes”. (3) “Ependymal pituicytes” are believed to provide ultrastructural evidence that human pituicytes are phylogenetically derived from ependymal cells. (4) “Oncocytic pituicytes” were observed in all cases and are of unknown significance. (5) The ultrastructural features of “granular pituicytes” suggest the existence of an active uptake and catabolism of extracellular material by pituicytes, probably through “ultraphagocytosis” or “endocytosis”. These five classes of pituicytes are considered to represent different functional forms of one cell line that originates phylogenetically from the ependyma.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 61
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 205 (1980), S. 361-370 
    ISSN: 1432-0878
    Keywords: Enamel organ ; Human foetus ; Tooth germ ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of external enamel epithelium, stellate reticulum and stratum intermedium in primary tooth germs (bell stage) from four human foetuses was investigated. Characteristically, the cells of the differentiated external enamel epithelium, stellate reticulum and stratum intermedium exhibit many free ribosomes, few rough endoplasmic reticulum cisterns, well-developed Golgi complexes, many coated and smooth vesicles, often in relation to the cell membranes, and many bundles of tonofilaments. The cells are connected by numerous desmosomes and gap junctions. A parallel differentiation of stratum intermedium — external enamel epithelium, and the ameloblast layer is demonstrated. The morphology of the cells of the three layers indicates that these have secretory, transport and supporting functions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 62
    ISSN: 1432-0878
    Keywords: Trophamnion ; Coelenterate, Polypodium hydriforme ; Ultrastructure ; Planula ; Parasitism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The larval stage of Polypodium hydriforme is planuliform and parasitic inside the growing oocytes of acipenserid fishes. The larva has inverted germ layers and a special envelope, the trophamnion, surrounding it within the host oocyte. The trophamnion is a giant unicellular provisory structure derived from the second polar body and performing both protective and digestive functions, clearly a result of adaptation to parasitism. The trophamnion displays microvilli on its inner surface, and irregular protrusions anchoring it to the yolk on its outer surface. Its cytoplasm contains long nuclear fragments, ribosomes, mitochondria, microtubules, microfilaments, prominent Golgi bodies, primary lysosomes, and secondary lysosomes with partially digested inclusions. The cells of the larva proper are poorly differentiated. No muscular, glandular, neural, interstitial, or nematocyst-forming cells have been found. The entodermal (outer layer) cells bear flagella and contain rough endoplasmic reticulum; the ectodermal (inner layer) cells lack cilia and contain an apical layer of acid mucopolysaccharid granules. The cells of both layers contain mitochondria, microtubules, and Golgi bodies; their nuclei display large nucleoli with nucleolonema-like structure, decondensed chromatin, and some perichromatin granules. At their apical rims, the ectodermal cells form septate junctions; laterally, the cells of both layers form simple contacts and occasional interdigitations. The lateral surfaces of entodermal cells are strengthened by microtubules.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 63
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 210 (1980), S. 257-267 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Smooth muscle ; Connective tissue ; Portal vein ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The smooth muscle of rabbit portal vein was studied by electron microscopy with particular emphasis on the mechanical linkage between the muscle cells and on the distribution of connective tissue. The media of this vein is composed of inner circular and outer longitudinal muscle layers which are orientated almost perpendicularly to each other. The muscle of the inner circular layer shows very irregular contours with much branching and anastomosing of the cytoplasmic processes, which often make membrane contacts with neighbouring cells to form an extensive network of cytoplasmic processes. The muscle cells of the outer longitudinal layer are arranged in densely packed bundles and are spindle-shaped, with no branching processes. Opposing dense areas from neighbouring cells, with variable gap distances (30–100 nm) and close membrane contacts (intermediate junctions) with a gap of 11 nm were observed in both circular and longitudinal muscle layers. In the terminal regions of muscle cells in both circular and longitudinal layers a specialized anchoring structure was present which was closely related to extracellular elastic tissue. Muscle cells in the longitudinal layer showed the most elaborate structure, the tapering end of the muscle cell showing a honeycomb-like structure penetrated by columns of connective tissue compounds. The functional implications of these structures are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 64
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 210 (1980), S. 339-347 
    ISSN: 1432-0878
    Keywords: Seasonal breeding ; Leydig cells ; Ultrastructure ; Bat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Leydig cells of the bat, Myotis adversus, have been examined by electron microscopy throughout fourteen months. During the breeding season the Leydig cells become hypertrophied and are characterised by prominent areas of agranular endoplasmic reticulum and numerous small, membrane-bound granules. Microperoxisomes are also observed. During the period of testicular regression. Leydig cell size and the number of membrane-bound granules are greatly reduced. Lipid droplets and dense bodies are more numerous.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 65
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 210 (1980), S. 417-433 
    ISSN: 1432-0878
    Keywords: Pinealocytes ; Ultrastructure ; Malaysian rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les pinéalocytes d'un Rat de Malaisie (Rattus sabanus), un animal vivant près de l'équateur donc dans une zone où les variations annuelles de la longueur des jours sont minimes, examinés au microscope électronique ont été comparés à ceux d'autres Mammifères. Une seule population de pinéalocytes a été observée. La présence d'un grand nombre de vésicules granulaires et de rubans circonscrits par des vésicules est la caractéristique premi`ere des pinéalocytes de cette espèce. Les rubans circonscrits par des vésicules ont été plus spécialement observés dans les terminaisons des prolongements des pinéalocytes où ils sont nombreux et la plus souvent en contact directe avec l'espace périvasculaire. L'importance physiologique de ces rubans circonscrits par des vésicules et des vésicules granulaires est discuté.
    Notes: Summary The ultrastructure of the pinealocytes of the Malaysian rat (Rattus sabanus), a mammal inhabiting a zone near the equator where the annual variations of daylength are inconspicuous, was examined and compared with that of pinealocytes of other mammals. On the basis of the presence of granular vesicles, only one population of pinealocytes was found. A large number of granular vesicles and vesicle-crowned rodlets is characteristic of the pinealocytes of this equatorial species. Vesicle-crowned rodlets are especially numerous in the endings of the pinealocyte processes and; they most often found in direct topographical connection with the perivascular spaces. The physiological significance of the presence of such large amounts of vesicle-crowned rodlets and of the secretory process characterized by the formation of granular vesicles is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 66
    ISSN: 1432-0878
    Keywords: Growth hormone-release-inhibiting factor (somatostatin) ; Sympathetic ganglia ; Ultrastructure ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The superior cervical ganglia (SCG), celiac superior mesenteric ganglia (CMG), and splanchnic nerve of unoperated guinea pigs, as well as both proximal and distal stumps of a previously transected branch of the postganglionic plexus of the CMG, were immunostained for somatostatin (SS). In addition, the PAP technique was adapted for fine-structural visualization of SS. A greater proportion of cells were labeled for SS in the CMG than in the SCG. PAP molecules were present in one type of intraganglionic axons. Only two labeled axons were found in the splanchnic nerve. Neither proximal nor the distal stump of the transected CMG postganglionic nerve contained labeled axons. The present results support the hypothesis that the intraganglionic axons labeled for SS arise from SS-containing intraganglionic neurons.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 67
    ISSN: 1432-0878
    Keywords: Sturgeon ; Muscle fibres ; Ultrastructure ; Morphometry ; Vascularisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure and vascular supply of the different fibre types in the lateral muscles of the sturgeon Acipenser stellatus were studied by light- and electron microscopy and morphometry. Three fibre types form separate layers without intermingling. The red fibres are superficial, the white fibres deep and the intermediate fibres between them. From morphometric analyses, the mitochondrial volume fraction in red fibres is 30%, in intermediate fibres 3.7% and in white fibres 0.7%. Z lines are most fuzzy in the red fibres. Triads of the sarcotubular system are always situated at the level of the Z discs. In red fibres the three elements are arranged in a series along the myofibrils, whereas in white fibres they are arranged transversely and in the intermediate fibres they are aligned obliquely. The number of capillaries surrounding each fibre is 2.3, 0.9 and 0.2 for the red, intermediate and white fibres, respectively. In red fibres 16% of the surface is directly covered by capillaries. The corresponding percentages for intermediate and white fibres are 5 and 1, respectively. Per unit volume of the fibre, the directly vascularised fibre surface in red fibres is about ten times larger than that of white fibres. The degree of vascularisation of the fibre types is directly related to the volume fraction of mitochondria, and thus to their aerobic capacities.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 68
    ISSN: 1432-0878
    Keywords: Calcergy ; Calcification ; Cutaneous calcinosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In rats a subcutaneous injection of KMnO4 leads to the calcification of the connective tissue. During mineral deposition, both intra- and extracellular changes are observed in the connective tissue. The intracellular phase is characterized by the formation of intramitochondrial granules and cytoplasmic vesicles, both in fibroblastic and extrinsic cells. In the extracellular phase, numerous heterogeneous matrix vesicles appear in the extracellular matrix. At the same time, globular particles which are resistant to microincineration, are observed between the collagen fibrils. The mineralization of the extracellular matrix takes place in two stages. The first stage comprises the appearance of needle-like structures and round aggregates. The needle-like structures are observed occasionally in the matrix vesicles and often in the extracellular matrix where they appear isolated or diverging from a central point. The round aggregates, composed of dense particles, are seen in the ground substance between the collagen fibrils. The second stage is characterized by a progressive mineralization of the collagen fibrils and the elastic fibers, without formation of extended calcified plaques.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 69
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 213 (1980), S. 221-235 
    ISSN: 1432-0878
    Keywords: Peripheral blood lymphocyte ; Density separation ; T cell depleted rabbits ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Density separation of purified peripheral blood leucocytes from T-cell depleted rabbits on a linear Ficoll-metrizoate gradient has been applied to obtain different leucocyte fractions. Two lymphocyte fractions separated on density seem to have different characteristics, both morphologically and immunologically. In this study these two fractions have been characterized ultrastructurally by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and a relationship has been established between the surface architecture (SEM), the cell size (SEM/TEM) and surface-Ig/C3-receptors (LM, light microscopy). Finally three types of lymphocytes have been described in the two lymphocyte fractions separated on density. Morphometric information such as cell size, cell shape, eu-/heterochromatin ratio in the nucleus and the nucleus-/cell ratio have been correlated to the stage of activation of the B lymphocyte in a representative density separation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 70
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 208 (1980), S. 183-196 
    ISSN: 1432-0878
    Keywords: Sinus node ; Atrium ; Ultrastructure ; Caveolae ; Freeze fracture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Caveolae or membrane vesicles are commonly observed in smooth and skeletal muscle as well as in working heart muscle. Using sections of fixed tissue and replicas of freeze-cleaved material, we show in this study that caveolae are also very numerous in sinus node cells of the rabbit, and to a lesser degree, in the atrial cells. Caveolae increase the plasma membrane surface area by 115% in the leading sinus node, and by 56% in the atrial cells. In these two cell types, the membrane of the caveolae contains four times fewer intramembranous particles than the rest of the plasma membrane, and this difference applies to both PF and EF faces. The role of the caveolae is still unclear, but it does not seem that they have a pinocytotic function.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 71
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 207 (1980), S. 171-182 
    ISSN: 1432-0878
    Keywords: Nephridia ; Anatomy ; Ultrastructure ; Tardigrada
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The excretory system of Macrobiotus richtersi consists of one dorsal and two lateral components and shows a high degree of structural complexity. In each of these a tricellular external lobe and a column can be distinguished, the two parts being connected distally. The surface of the lobe cells is increased by deep basal infoldings and fingerlike processes which form a labyrinth next to the basal lamina. Their cytoplasm contains numerous mitochondria, a well developed rough endoplasmic reticulum, dictyosomes, and granules in amounts depending on the physiological state of the animal. Excretory crystals occur in caveolae located in the lobe: between the fingershaped processes of the cell and in the space enclosed by the basal lamina on one side and the column on the other. The column faces an extracellular channel meandering along its whole length which is surrounded on the outside by a basal lamina. Morphologically the column is similar to the protonephridial channel of Rotifera. At the ultrastructural level, the cytoplasm of the column shows numerous mitochondria, rough endoplasmic reticulum, lysosomes, and a well developed Golgi apparatus. The lumen of the channel is coated by glycocalyx. At the base of the column several small cells form the proximal part of a duct that communicates with the gut. The morphology and ultrastructure of the excretory system of M. richtersi have been compared with similar a system in Isohypsibius megalonyx (Greven, 1979), and on these grounds a proposal is put forward to call the excretory organs of Tardigrada “nephridia” instead of “Malpighian tubules”.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 72
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 210 (1980), S. 85-94 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Domestic fowl ; Preoptic area ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Numerous secretory parvocellular perikarya were found in the preoptic region of the domestic fowl (Gallus gallus). The dense-core secretory vesicles belong to two categories: vesicles with a diameter of (i)80–90 nm and (ii) 110–140nm. Scattered magnocellular elements display larger dense-core granules. The parvocellular neurons form unit-like clusters, showing also zones of direct apposition of neuronal membranes. The surrounding neuropil is rich in synaptic structures, formed by at least three types of axon terminals, distinguishable on the basis of vesicular morphology. These observations confirm the findings in other avian species. The hypothetical function of this system of peptidergic neurons in the rostral hypothalamus of birds is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 73
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 210 (1980), S. 121-132 
    ISSN: 1432-0878
    Keywords: Glycoproteins ; Ultrastructure ; Tunicamycin ; Colonic epithelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Glycoproteins are associated with several structures of colonic absorptive cells of the mouse. These include the cell coat, Golgi apparatus and vesicles that transport the glycoproteins from the apparatus to the cell surface (Michaels and Leblond 1976). In many in vitro systems, the antibiotic tunicamycin inhibits the glycosylation of asparagine residues yielding carbohydrate-poor glycoproteins. In the present in vivo study, tunicamycin was injected into mice. The murine colonic epithelial cells were prepared routinely for electron microscopy and cytochemistry. Cells from the experimental and control animals were similar morphologically. However, staining by the periodic acid-chromic acid-silver methenamine technique, revealed differences in the distribution of glycoproteins. In animals that received the higher dosages of tunicamycin there was a substantial reduction in silver staining in both the Golgi apparatus and the vesicles of colonic epithelial cells compared to these structures in cells of identically treated control tissues, whereas the staining over the cell coat was not significantly altered. Possible explanations for the staining of the cell coat in the treated animals were provided in the text. This report demonstrates the feasibility of using tunicamycin in vivo and detection of the changes obtained by the silver methenamine method.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 74
    ISSN: 1432-0878
    Keywords: Transmitter-dualism ; Multipotential neuron ; Heart innervation ; Ultrastructure ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The segmental heart nerves of Caligo beltrao Illiger (Brassolidae) were examined by transmission and scanning electron microscopy. Heart and alary muscles are innervated by branching processes of single multinucleate neurons (MNNs). There is one MNN situated at each segmental fan-shaped group of alary muscles. The main nerve of the MNN consists of a bundle of processes. This nerve extends centripetally toward the CNS and corresponds to the dorsal portion of the transverse nerve. However, neither axo-somatic nor axo-axonic synapses were found, the presence of which might suggest that this nerve contains axons of different neuronal origin. The synaptic contacts of the MNN with axons originating from the CNS are therefore assumed to be established beyond the spiracular region. In addition to the neuro-muscular junctions of the smaller centrifugal axon branches there are neurohaemal release sites along the entire length of all MNN axon bundles. Axon terminals are packed with either dense-cored or multigranular vesicles. Both morphological types of vesicles are, however, found side by side in the large axons and in the perikaryon, often at the same Golgi element. These morphological findings may support the concept that more than one transmitter is produced in a single neuron. Questions that arise in reference to dual or polyfunctional neurons and to the control of cardiac activity are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 75
    ISSN: 1432-0878
    Keywords: Cerebellum ; Purkinje axon ; Ultrastructure ; Axotomy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transection of Purkinje cell axons in adult male rats made 1.5 mm or further from the cell body does not lead to the death of the neuron and results in compensatory structural alterations of the surviving axonal portions of the nerve cell. Near to, and at the emergence of recurrent collaterals of Purkinje cell axons, huge varicosities filled with filaments, granular material, lysosomes and mitochondria develop. Terminals of recurrent axon collaterals also exhibit different degrees of structural changes. Most striking of the morphological alterations is the regular presence of nematosomes in the hypertrophic axonal branches, especially in synaptic terminals. Since nematosomes were shown to contain RNA in other types of neurons, their presence in recurrent collaterals may indicate an enhanced synthetic activity in Purkinje axonal processes and endings after axotomy.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 76
    ISSN: 1432-0878
    Keywords: Ecdysteroids ; Moulting hormones ; Xyleborus ferrugineus pupae ; Ultrastructure ; Prothoracic gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The prothoracic glands of female pupae of Xyleborus ferrugineus at three ages (0-h, 48-h and 72-h-old) were examined for ultrastructural changes that correlate with high titers of ecdysteroids in the entire pupa. In all three ages, the prothoracic gland cells appear compact with a prominent nucleus. Lysosome-like structures with concentrically oriented internal membrane are observed in both 0-h and 72-h-old gland cells. Mitochondria are abundant in the glands of both ages, and rough endoplasmic reticulum is relatively sparse. Numerous microtubules are present in the prothoracic gland cells of 48-h-old female pupae that contain relatively high titers of ecdysteroids. Rough endoplasmic reticulum also is especially abundant at this age of the pupa. Ecdysone titers, estimated by the radioimmunoassay method (RIA), were 161.04±22.61 pg/mg, 704.25±69.02 pg/mg and 298.72±26.80pg/mg body weight in 0-h, 48-h and 72-h-old female pupae, respectively.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 77
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 213 (1980), S. 149-157 
    ISSN: 1432-0878
    Keywords: Trophic chamber ; Nutritive cord ; Ultrastructure ; Homoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Each ovariole of the coccidian Aspidiotus hederae contains a single oocyte connected by means of a nutritive cord to the trophic chamber. The trophic chamber consists of three nurse cells characterized by an enlarged, ramified nucleus with a prominent nucleolus. The perinuclear cytoplasm contains nuage material, large amounts of free ribosomes, and scattered mitochondria. Occasional cisternae of the rough endoplasmic reticulum and bacteroids are found in trophocyte cytoplasm. The nutritive cord contains many microtubules in parallel array interspersed with numerous free ribosomes and a few mitochondria. The nutritive cord is strengthened by trophocyte projections which surround it. Microtubules in the projections are oriented perpendicular to the long axis of the cord.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 78
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 90 (1980), S. 1245-1247 
    ISSN: 1573-8221
    Keywords: host specificity system ; restriction ; modification ; DNA ; cross titration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 79
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 90 (1980), S. 1424-1426 
    ISSN: 1573-8221
    Keywords: 5-Fluorouracil ; cyclophosphamide ; combined therapy ; DNA ; RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 80
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 90 (1980), S. 1058-1060 
    ISSN: 1573-8221
    Keywords: histones ; DNA ; histone-DNA interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 81
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 90 (1980), S. 1239-1242 
    ISSN: 1573-8221
    Keywords: cyclic AMP ; biosynthesis ; RNA ; DNA ; collagen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 82
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 89 (1980), S. 774-776 
    ISSN: 1573-8221
    Keywords: DNA ; systemic lupus erythematosus ; pronase ; hydroxyapatite ; cryoprecipitins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 83
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of experimental biology and medicine 90 (1980), S. 1082-1085 
    ISSN: 1573-8221
    Keywords: hypertension ; adrenalectomy ; fat cells ; isoproterenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 84
    ISSN: 1573-3297
    Keywords: mating success ; allozyme polymorphism ; overdominance ; mating latency ; Adh locus ; Drosophila melanogaster ; rare genotype mating advantage ; alcohol dehydrogenase ; statistical methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Strains ofDrosophila melanogaster having different alcohol dehydrogenase (Adh) genotypes (FF, FS, or SS) were assembled in a mating chamber in varying ratios, and the mating successes were recorded. In experiments with a 25∶25 ratio, theFF males succeeded in mating more than didFS andSS males, while theFS males surpassed theSS males. As for the females,FF also surpassedSS. In experiments with a 5∶45 or 45∶5 ratio, some differences from the 25∶25 ratio occurred, but in these cases the rare genotypes were at a disadvantage. In one case, female genotypes (FF vs.SS) displayed a difference in mating latency time, but male genotypes did not. The findings did not suggest that rare genotype mating advantage and overdominance in mating success play a role in the maintenance of theAdh polymorphism.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 85
    Electronic Resource
    Electronic Resource
    Springer
    Behavior genetics 10 (1980), S. 291-302 
    ISSN: 1573-3297
    Keywords: foraging strategies ; chromosomal analysis ; Drosophila melanogaster ; larvae ; feeding-locomotor behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Two larval foraging strategies inDrosophila melanogaster were identified, “rover” and “sitter.” “Rovers” traverse a large area while feeding whereas “sitters” cover a small area. The difference between “rovers” and “sitters” was analyzed genetically by chromosomal substitutions between isogenic stocks. Differences in larval locomotor behavior (“crawling behavior”) can be attributed to the second chromosome, the “rover” strategy being dominant over the “sitter” strategy. Differences in feeding rate (“shoveling behavior”) are affected additively by both the second and third chromosomes. Natural populations ofDrosophila larvae were sampled three times over a 2-month period; “rovers” and “sitters” were at constant frequencies in these populations. The two foraging strategies are discussed in the light of resource utilization in environments where food is distributed continuously or discontinuously.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 86
    Electronic Resource
    Electronic Resource
    Springer
    Plant systematics and evolution 136 (1980), S. 267-273 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Daucus andPetroselinum (Apiaceae) ; Datura (Solanaceae) ; DNA ; reassociation kinetics ; DNA hybridization between varieties of the same species ; and genera of the same and of different families
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Reassociation kinetics ofDaucus carota andPetroselinum crispum (Apiaceae), andDatura innoxia (Solanaceae) are presented. Hybridization of3H-labelled DNA of two carrot cultivars indicate strong qualitative homologies of DNA sequences; nevertheless, certain quantitative differences in some Cotregions seem to exist. However, homologous sequences ofDaucus DNA with DNA ofDatura, and, suprisingly, even with DNA ofPetroselinum are very restricted: between 8% in the repeated regions and ca. 7–9% in the unique regions.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 87
    ISSN: 1573-5060
    Keywords: Triticale ; Staining ; Banding ; Chromosome ; DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifteen lines of triticale were studied using the C-banding staining method. A typical, complete chromosomal complement was found in 11 lines, while the remaining lines demonstrated some variations. A slight modification in the technique is discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 88
    ISSN: 1432-1955
    Keywords: Flagellates ; Spironucleus ; Mouse ; Ultrastructure ; Systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Using light and electron microscopy, we studied the ultrastructure ofSpironucleus muris (syn.Hexamita muris) from spontaneously and experimentally infected normal and athymic mice. Kinetosomes and cytoskeletal fibers arranged in two-fold rotational symmetry are similar to the speciesSpironucleus elegans from amphibians. Taxonomic proposals and a possible evolutionary scheme for diplomonad genera accepted at the last International Congress of Parasitology are given. The genusHexamita should be divided into two new genera:Hexamita (usually free-living) andSpironucleus (exclusively parasitic). We consider previous descriptions of hexamitiasis in rodents as dealing with spironucleosis. We distinguish a fresh and an old cyst of the parasite on the basis of structure, light refraction, location in the host, and infectivity. The transition from a fresh to an old cyst paralles the dessication of feces. The trophozoites can damage the microvilli and penetrate into the epithelium. We explain the differences in findings concerning intestinal lesions and forms of the disease (from acute to chronic to latent) by differences in the virulence of different parasite strains and by differences in inherent host resistance.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 89
    ISSN: 1432-1955
    Keywords: Cestoda ; Proteocephalus tidswelli ; Ultrastructure ; Microtriches
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ultrastructural observations on adultProteocephalus tidswelli revealed a marked microtrichial polymorphism. Structural and dimensional variations of microtriches between different regions of the strobila and scolex, as well as within the same region, were observed. The authors suggest that microtriches are involved in a diversity of functions, and possible functional activities are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 90
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 63 (1980), S. 33-46 
    ISSN: 1432-1955
    Keywords: Parorchis acanthus ; Redia ; Ultrastructure ; Migration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Rediae ofParorchis acanthus were examined by scanning electron microscopy and the ultrastructure of the surface related to migration through the digestive gland ofNucella lapillus. The median ventral birth papilla, ventro-lateral processes, and posterior papilliform process, of young active rediae, apparently serve to anchor the body during migration. The honeycomb-like apex of the birth papilla and ventro-lateral processes may be a strengthening device. Uniciliate sensory receptors around the mouth, on the birth papilla, and posterior terminal papilla are probably touch receptors or chemoreceptors concerned with feeding and orientation during migration. After the formation of the birth pore, and with increase in size and in the number of contained cercariae, the redia becomes less active, loses its characteristic shape, and is eventually immobile.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 91
    ISSN: 1432-1955
    Keywords: Schistosoma mansoni ; Mother sporocyst ; Daughter sporocyst ; Biomphalaria pfeifferi ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The development of the mother sporocyst and the differentiation of the daughter sporocyst ofSchistosoma mansoni inBiomphalaria pfeifferi are described. The tegumental structure of the mother sporocyst, consisting of an outer layer connected to internally situated nucleated cell bodies, forms extensions which enwrap the germinal cells. The parenchyma cells, in which the germinal cells were embedded before, degenerate. When daughter sporocyst embryos develop from germinal cells they are enveloped by a primitive epithelium which is formed by fusion of the extensions of the tegumental structure of the mother sporocyst. Somatic cells located peripherally in the developing daughter sporocyst expand and coalesce beneath the primitive epithelium to form the future outer layer of the tegumental structure of the daughter sporocyst. The primitive epithelium degenerates, the newlyformed layer looses its nuclei, and becomes connected to internally situated nucleated cell bodies. Further developments in the tegumental structure of the daughter sporocyst include the formation of microvillus-like projections, a surface coat, spines, and a basement membrane.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 92
    ISSN: 1432-1955
    Keywords: Ultrastructure ; Microsporidia ; Simuliidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract During an investigation of the parasites of simuliid larvae in a defined area in Southern Germany six species of Microsporidia were found. These parasites infect larvae of six different species of Simuliidae. Using light and scanning electron microscopic techniques the microsporidians were identified asAmblyospora bracteata, Thelohania fibrata, T. varians, Pleistophora multispora, P. simulii, andP. debaisieuxi. The arrangement of membranes in the cyst as well as the surface structure of the spores are described.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 93
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 62 (1980), S. 63-74 
    ISSN: 1432-1955
    Keywords: Insect salivary gland ; Trypanosoma rangeli ; Penetration ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ultrastructural studies of the mechanism of penetration of the salivary gland of the reduviid bugRhodnius prolixus byTrypanosoma rangeli showed that trypanosomes from the haemocoele penetrate the outer “membranes” of the gland flagellum foremost, disrupting the inner layers, to pass between the muscle cells to reach the gland cell basement membrane. This latter is also penetrated flagellum foremost, the parasite invaginating the gland cell plasmalemma beneath, to create a vacuole in which the trypanosome crosses the gland cells to reach the central lumen, often only losing its containing vacuole just before leaving the cell. The structure of the outer “membranes” surrounding the salivary gland appeared similar to, and often actually part of, the basement membrane of the gland cells. These outer “membranes” were found to enclose large numbers of multinuleate “giant form” trypanosomes, whose significance is as yet unknown, but could perhaps represent a stage in the life cycle of the parasite where genetic interchange could take place.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 94
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 63 (1980), S. 233-239 
    ISSN: 1432-1955
    Keywords: Siphonaptera ; Leptomonas ; Ultrastructure ; Cysts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ultrastructural studies onLeptomonas ctenocephali ofCtenocephalides canis andLeptomonas species ofOrchopeas h. howardi andPalaeopsylla m. minor have demonstrated the presence of characteristic round and oval parasites with a regularly indented plasma membrane. Such forms were found only as unattached parasites in the lumen of the pylorus, hindgut, and rectum amongst parasites which showed a normal kinetoplastid plasmalemma. The plasmalemma indentations are regular in their distribution around the periphery of the parasite, being separated usually by a fixed number of subpellicular microtubules in each “cyst-like form”, some subpellicular microtubules are displaced from the periphery at the point where the plasmalemma is indented furthest into the cytoplasm. It is suggested that these forms meet the criteria necessary to be characterised as “cyst-like forms” despite the apparent absence of a true cyst wall.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 95
    Electronic Resource
    Electronic Resource
    Springer
    Parasitology research 64 (1980), S. 1-15 
    ISSN: 1432-1955
    Keywords: Intersexuality ; Pedicel of antennae ; Chironomus ; Limnomermis rosea ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Der normale Bau der Scolopidialorgane in den Pedicelli der Antennen vonChironomus entspricht dem vonAedes, mit Ausnahme folgender Besonderheiten: a) Der Geschlechtsdimorphismus des Organes ist ausgeprägter als beiAedes. b) Das Pedicelluslumen ist lateral und ventral größer als medial und dorsal. c) Der Bau des Geißelgelenkes ist einfacher, die innere Pedicelluswand massiv. d) Die Spangen des männlichen Pedicellus sind abwärts gebogen, die Scolopidien des proximalen Ringes setzen von oben und unten an den Spangen an, 2–4 der unteren Scolopidien jeder Spange haben größeren Durchmesser. Die Scolopidienzahl im proximalen Ring der ♀ ist viel geringer als beiAedes. e) Das Zentralorgan ist bei beiden Geschlechtern gleich, seine Scolopidien entsprechen Typ D vonAedes (Boo und Richards 1975a, b). Bei ♂ vonC. anthracinus Zett., die vonLimnomermis rosea Hagm. parasitiert sind, entspricht der Pedicellus völlig dem normaler und parasitierter ♀ (Tabelle 1). Werden ♂ vonC. riparius Meig. oderC. luridus Str. mit dem gleichen Parasiten infiziert, so kann der Pedicellus intersex abgewandelt sein: Der Grad der distalen Einsenkung, der Bau des Flagellum-Pedicellus-Gelenkes, die Spangenzahl und die Scolopidienzahl im proximalen Ring können offenbar alle Zwischenstufen zwischen männlicher und weiblicher Ausprägung erreichen. Der Übergang männchenähnlicher und weibchenähnlicher Bereiche in solchen Pedicelli erscheint abrupt. Die Ultramorphologie der Scolopidien bleibt vom Parasiten unbeeinflußt.
    Notes: Abstract The normal morphology of the scolopidial organs in pedicels ofChironomus antennae corresponds to that ofAedes, with the following exceptions: (a) The sexual dimorphism of the organ is more pronounced than inAedes. (b) The lumen of the pedicel is larger at its lateral and ventral side than at its median and dorsal side. (c) The construction of the flagellar side than at its median and dorsal side. (c) The construction of the flagellar prongs in male pedicels are directed downwards. The scolopidia of the proximal ring are attached to the upper and lower side of the prongs, 2–4 of the lower scolopidia of each prong have a greater diameter. The number of scolopidia in the proximal ring of females is much less than inAedes. (e) The central organ is identical in both sexes, its scolopidia correspond to type D ofAedes (Boo and Richards 1975 a, b). Pedicel structures inChironomus anthracinus males parasitized byLimnomermis rosea are identical to those of normal and parasitized females (Table 1). IfC. riparius orC. luridus males are parasitized by the same nematode, pedicels are often intersexual: The degree of the distal depression, construction of the flagellar articulation, number of prongs, and number of scolopidia in the proximal ring may attain all intergrades between maleness and femaleness. The transition between male- and female-like parts of the pedicel is abrupt. The ultramorphology of the scolopidia, however, is not influenced by the parasite.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 96
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 205 (1980), S. 67-76 
    ISSN: 1432-0878
    Keywords: Starfish egg ; Cortical reaction ; Vitelline coat ; Fertilization envelope ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The egg coats of a starfish (Patiria miniata) are examined before, during, and after the cortical reaction by scanning and transmission electron microscopy. The unfertilized egg is closely invested by a vitelline coat about 300 μm thick, and cortical granules are scattered in the peripheral cytoplasm. After insemination, as the cortical granules undergo exocytosis, the cortical reaction sweeps over the egg surface. Much of the material ejected from the cortical granules adheres to the inner surface of the vitelline coat as a dense layer about 40 μm thick and as scattered spheres and hemispheres, each about 1 μm in diameter. Together, the vitelline coat and the adherent cortical granule material form the fertilization envelope, which becomes separated from the plasma membrane of the egg by a perivitelline space. The perivitelline space contains some flocculent material, which is too diffuse and discontinuous to be considered a hyaline layer. Possible functions of the starfish egg coats are discussed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 97
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 205 (1980), S. 85-94 
    ISSN: 1432-0878
    Keywords: Plasma cells ; Lymphocytes ; Macrophages ; Harderian gland ; Ultrastructure ; Birds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the subepithelial layer of the Harderian gland of some birds, macrophages, lymphocytes, plasma cells, as well as granulocytes are found. These cells are often densely aggregated in the central regions of the lobules, while individual cells occur near the periphery. Pre-plasma cells form desmosome-like contacts with macrophages and junctional complexes with each other. The lymphoid cells are sometimes embedded in a dense homogeneous material, which is also found in the intercellular spaces of the secretory epithelium. The present findings are discussed with reference to immunological aspects.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 98
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 205 (1980), S. 133-145 
    ISSN: 1432-0878
    Keywords: Lung ; Amphibia ; Ultrastructure ; Morphometry ; Atypical mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The lungs of Triturus alpestris Laur. were investigated with the scanning and transmission electron microscopes. Dimensions of the cell bodies of pneumocytes and ciliated cells, as well as the thickness of the air-blood barrier, were determined. The lungs of the newt form two simple sacs without septa. A ciliated epithelium containing goblet cells lines the pulmonary vein and partially the pulmonary artery. The remainder of the lung surface is covered internally by respiratory epithelium consisting of one type of cell and only occasionally showing the presence of single ciliated cells. All cells, ciliated, goblet and pneumocytes, contain in their cytoplasm lamellar bodies. Multivesicular bodies and numerous vesicles of variable electron density also occur in the cytoplasm of pneumocytes. Atypical mitochondria can be found in all cell types of the lung. Fixation with addition of tannic acid reveals the surface lining film. Tubular myelin figures were not observed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 99
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 205 (1980), S. 253-271 
    ISSN: 1432-0878
    Keywords: Neurohypophysis ; Neurosecretory axons ; Neurosecretorygranules ; Ultrastructure ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neurosecretory axons and their dilatations in the pars nervosa of the human neurohypophysis were studied electron microscopically. The axons are of two different types based on their content of neurosecretory granules (NSGs): (i) NSGs of Type A are 100–300 nm, and (ii) NSGs of type B are 50–100 nm in diameter. While fibers (or axons) of type B were scarce, showing simple swellings and terminal formations, fibers of type A were ubiquitous in the human pars nervosa, exhibiting numerous dilatations with a diversity of internal structure, apparently representing the ultrastructural manifestation of intraaxonal turnover of neurohypophysial hormones. Based on the predominating aspect of their internal structure, dilatations of type A-fibers were classified into six different types, with various transitional forms: Type I is characterized by abundant NSGs; type II by prominent mitochondria; type III by abundant lysosomal bodies; type IV by an electron-lucent matrix with few organelles; type V by prominent tubuloreticular profiles; and type VI by numerous microvesicles. The functional significance of each type is discussed and a scheme of possible interrelationships between these dilatations is proposed.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 100
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 205 (1980), S. 371-382 
    ISSN: 1432-0878
    Keywords: Ameloblast ; Amelogenesis ; Human foetus ; Tooth germ ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of internal enamel epithelium, preameloblasts and secretory ameloblasts in primary tooth germs (bell stage) from four human foetuses was investigated. The characteristics of the differentiation of internal enamel epithelium via preameloblasts to secretory ameloblasts are described. The internal enamel epithelium consists of a row of low differentiated prismatic cells separated from the dental papilla by a distinct even basal lamina. In the preameloblasts the rough endoplasmic reticulum cisterns and mitochondria increase in number, the Golgi complexes become extensive and take up a distal position, and secretory granules are formed. Furthermore, the basal lamina is removed by coated vesicles, and proximally and distally in the cells a complex of zonulae adhaerentes, terminal webs and gap junctions is formed. The secretory ameloblasts make up a layer of highly differentiated cells demonstrating typical merocrine secretion.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...