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Alterations in the distribution of glycoproteins in epithelial cells of murine colon after injection of tunicamycin

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Summary

Glycoproteins are associated with several structures of colonic absorptive cells of the mouse. These include the cell coat, Golgi apparatus and vesicles that transport the glycoproteins from the apparatus to the cell surface (Michaels and Leblond 1976). In many in vitro systems, the antibiotic tunicamycin inhibits the glycosylation of asparagine residues yielding carbohydrate-poor glycoproteins. In the present in vivo study, tunicamycin was injected into mice. The murine colonic epithelial cells were prepared routinely for electron microscopy and cytochemistry. Cells from the experimental and control animals were similar morphologically. However, staining by the periodic acid-chromic acid-silver methenamine technique, revealed differences in the distribution of glycoproteins. In animals that received the higher dosages of tunicamycin there was a substantial reduction in silver staining in both the Golgi apparatus and the vesicles of colonic epithelial cells compared to these structures in cells of identically treated control tissues, whereas the staining over the cell coat was not significantly altered. Possible explanations for the staining of the cell coat in the treated animals were provided in the text. This report demonstrates the feasibility of using tunicamycin in vivo and detection of the changes obtained by the silver methenamine method.

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References

  • Bennett G, Leblond CP, Haddad A (1974) Migration of glycoprotein from the Golgi apparatus to the surface of various cell types as shown by radioautography after labeled fucose injection into rats. JCell Biol 60:258–284

    Google Scholar 

  • Digglemann H (1979) Biosynthesis of an unglycosylated envelope glycoprotein of Rous sarcoma virus in the presence of tunicamycin. J Virol 30:799–804

    Google Scholar 

  • Duksin D, Bornstein P (1977) Changes in surface properties of normal and transformed cells caused by tunicamycin, an inhibitor of protein glycosylation. Proc Natl Acad Sci 74:3433–3437

    Google Scholar 

  • Fujisawa J, Iwakura Y, Kawade Y (1978) Nonglycosylated mouse L cell interferon produced by the action of tunicamycin. J Biol Chem 253:8677–8679

    Google Scholar 

  • Goldstone A, Koenig M (1970) Lysosomal hydrolases as glycoproteins. Life Sci Part 2 9:1341–1350

    Google Scholar 

  • Kornfeld R, Kornfeld S (1970) The structure of a phytohemagglutinin receptor site from human erythrocytes. J Biol Chem 245:2536–2545

    Google Scholar 

  • Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414

    Article  CAS  PubMed  Google Scholar 

  • Mahoney WG, Duksin D (1979) Biological activities of the two major components of tunicamycin. J Biol Chem 254:6572–6576

    Google Scholar 

  • Michaels JE (1977) Glycoprotein containing vesicles in the surface epithelial cells of the ascending colon of the rat. Anat Rec 188:525–534

    Google Scholar 

  • Michaels JE, Leblond CP (1976) Transport of glycoprotein from Golgi apparatus to cell surface by means of “carrier” vesicles as shown by radioautography of mouse colonie epithelium after injection of 3H-fucose. J Microsc Biol Cell 25:243–248

    Google Scholar 

  • Morrison TG, McQuain CO, Simpson D (1978) Assembly of viral membranes: maturation of the vesicular stomatitis virus glycoprotein in the presence of tunicamycin. J Virol 28:368–374

    Google Scholar 

  • Nishikawa Y, Yamamoto Y, Onodera K, Tamura G, Mitoui H (1979) Altered molecular structure of HLA-DR antigens synthesized in the presence of tunicamycin. Biochem Biophys Res Comm 87:1235–1242

    Google Scholar 

  • Olden K, Pratt RM, Yamada KM (1978) Role of carbohydrates in protein secretion and turnover: effects of tunicamycin on the major cell surface glycoproteins of chick embryo fibroblasts. Cell 13:461–413

    Google Scholar 

  • Rambourg A (1967) An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope. J Histochem Cytochem 15:409–412

    Google Scholar 

  • Rambourg A (1971) Morphological and histochemical aspects of glycoproteins at the surface of animal cells. Int Rev Cytol 31:57–113

    Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212

    Article  CAS  PubMed  Google Scholar 

  • Rosen OM, Chia GH, Fung C, Rubin CS (1979) Tunicamycin-mediated depletion of insulin receptors in 3T3-L1 adipocytes. J Cell Physiol 99:37–42

    Google Scholar 

  • Schneider EG, Nguyen HT, Lennarz WJ (1978) The effect of tunicamycin, an inhibitor of protein glycosylation, on embryonic development in the sea urchin. J Biol Chem 253:2348–2355

    Google Scholar 

  • Schultz AM, Orozlan S (1979) Tunicamycin inhibits glycosylation of precursor polyprotein encoded by env gene of Rauscher murine leukemia virus. Biochem Biophys Res Comm 86:1206–1213

    Google Scholar 

  • Takatsuki A, Arima K, Tamura G (1971) Tunicamycin, a new antibiotic. I. Isolation and characterization of tunicamycin. J Antibiot 24:215–231

    Google Scholar 

  • Thomas DB, Winzler RJ (1969) Structural studies on human erythrocyte glycoproteins. Alkali labile oligosaccharides. J Biol Chem 244:5943–5946

    Google Scholar 

  • Waechter CJ, Harford JB (1977) Evidence for the enzymatic transfer of N-acetylglycosamine from UDP-N-acetylglucosamine into dolichol derivatives and glycoproteins by calf brain membranes. Arch Biochem Biophys 181:185–198

    Google Scholar 

  • Waechter CJ, Lennarz WJ (1976) The role of polyprenol-linked sugars in glycoprotein synthesis. Ann Rev Biochem 45:95–112

    Google Scholar 

  • Watson ML (1958) Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol 4:475–478

    Google Scholar 

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Michaels, J.E. Alterations in the distribution of glycoproteins in epithelial cells of murine colon after injection of tunicamycin. Cell Tissue Res. 210, 121–132 (1980). https://doi.org/10.1007/BF00232148

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  • DOI: https://doi.org/10.1007/BF00232148

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