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  • Articles  (326)
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  • Springer  (326)
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  • 1
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    Electronic Resource
    Springer
    Molecular and cellular biochemistry 136 (1994), S. 11-22 
    ISSN: 1573-4919
    Keywords: magnesium ; calcium ; mag-fura-2 ; fura-2 ; magnesium regulation ; BC3H-1 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The magnesium buffer coefficient (B Mg) was calculated for BC3H-1 cells from the rise in cytosolic Mg2+ activity observed when magnesium was released from ATP after iodoacetate (IAA) and NaCN treatment. The basal cytosolic Mg2+ activity (0.54±0.1 mM) measured with mag-fura-2 doubled when 4.54 mM magnesium was liberated from ATP:B Mg was 12.9 indicating that a 1 mM increase in Mg2+ activity requires an addition of about 13 mM magnesium. The accuracy of this value depends on these assumptions: (a) all of the magnesium released from ATP stayed in the cells; (b) the rise in Mg2+ was not secondary to pH-induced changes inB Mg; (c) mag-fura-2 measured Mg2+ and not Ca2+; and (d) the accuracy of the mag-fura-2 calibration. Total magnesium did not change in response to IAA/CN treatment, thus the change in Mg2+ activity reflected a redistribution of cell magnesium. pH changes induced by NH4Cl pulse and removal had little effect on Mg2+ activity and the changes were slower than and opposite to pH-induced changes in Ca2+ activity measured by fura-2. Ca2+ responses were temporally uncopled from Mg2+ responses when the cells were treated with IAA only and in no cases did Ca2+ levels rise above 1 μM, showing that the mag-fura-2 is responding to Mg2+. Additional studies demonstrated that ∼90% of the mag-fura-2 signal was cytosolic in origin. The remaining non-diffusible mag-fura-2 either was bound to cytosolic membranes or sequestered in organelles with the fluorescence characteristics of the Mg2+-complexed form, even when cytosolic free Mg2+ activity was approximately 0.5 mM. This bound mag-fura-2 would appear to increase the Kd and thus clearly limits the accuracy of our estimmate forB Mg. Despite this limitation, we demonstrate that Mg2+ is tightly regulated in face of large changes in extracellular Mg2+, and that the interplay observed between pH, Ca2+ and Mg2+ activities strongly supports the hypothesis that these factors interact through a shared buffer capacity of the cell.
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  • 2
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    Molecular and cellular biochemistry 135 (1994), S. 79-88 
    ISSN: 1573-4919
    Keywords: calcium ; nucleus ; calpain ; calmodulin ; cell division ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Calcium has long been known to play a role as a key cytoplasmic second messenger, but until relatively recently its possible involvement in nuclear signal transduction and the regulation of nuclear events has not been extensively studied. Evidence revealing the presence of transmembrane nuclear Ca2+ gradients and a variety of intranuclear Ca2+ binding proteins has fueled renewed interest in this key ion and its involvement in cell-cycle timing and division, gene expression, and protein activation. This review will offer an overview of the current state of knowledge and theory regarding calcium orchestration of nuclear functions and events and discuss possible future directions in this field of study.
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  • 3
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    Molecular and cellular biochemistry 135 (1994), S. 1-9 
    ISSN: 1573-4919
    Keywords: calcium ; contraction ; artery ; smooth muscle ; actin ; myosin ; phosphorylation ; calmodulin ; myosin light chain kinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The fact that smooth muscle exists in almost every hollow organ and is involved in a large number of disease states has led to a vast increase in smooth muscle research, covering areas from testing response to antagonists and agonists to measuring the molecular force generated by a single actin filament. Yet, the exact mechanisms regulating contractile response of smooth muscle remain unsolved. Calcium has been a central player in mediating smooth muscle contraction through binding with calmodulin, although there is evidence showing that under special circumstances smooth muscle can contract without change in intracellular Ca2+. In addition to the major regulatory pathway of Ca2+-calmodulin-mysoin light chain kinase, there are other thin filament linked regulatory mechanisms in which Ca2+-calmodulin dependent phosphorylation of calponin and caldesmon may be involved. Ca2+ sensitivity of smooth muscle contraction may vary under different situations and this has recently been recognized as an important regulatory mechanism. Examples are protein kinase C (PKC) dependent phosphorylation of myosin light chain kinase which results in partial inhibition of contraction, and activation of myosin light chain phosphatase. There is new evidence howing that not only does Ca2+ regulate contraction by regulating the interaction of contractile proteins in smooth muscle, but also that shortening of smooth muscle itself reduces intracellular Ca2+ concentration, via a negative feedback.
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  • 4
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    Molecular and cellular biochemistry 130 (1994), S. 137-147 
    ISSN: 1573-4919
    Keywords: microtubules ; calcium ; colchicine ; posttranslational modifications ; fish ; cow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Microtubule proteins were isolated by a temperature-dependent assembly-disassembly method from brain tissue of for cold-temperate fish; one fresh water fish (Oncorhynchus mykiss), and three marine fish (Labrus berggylta, Zoarces viviparus andGadus morhua). The α-tubulins from all four fish species were acetylated. The α-tubulins from the marine fish were composed of a mixture of tyrosinated and detyrosinated tubulin, while the fresh water fish tubulin only reacted with an antibody against detyrosinated tubulin. The isolated microtubules had a similar MAP composition. A 400 kD protein and a MAP2-like protein were found, but MAP1 was missing. All microtubules disassembled upon cooling to 0°C. In spite of these common characteristics, the assembly of microtubules fromLabrus berggylta was inhibited by colchicine and calcium, in contrast to the assembly of microtubules fromOncorhynchus mykiss andZoarces viviparus. For the latter, colchicine was not completely inhibitory even at a concentration as high as 1 mM, and calcium induced the formation of both loosely and densely coiled ribbons. The effects of calcium and colchicine on microtubules fromOncorhynchus mykiss andZoarces viviparus were modulated by either fish or cow MAPs, indicating that the effects are due to intrinsic properties of the fish tubulins and not the MAPs. In view of these findings, our results suggest that there is not correlation between colchicine sensitivity, inability of calcium to inhibit microtubule assembly, and acetylation and detyrosination.
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  • 5
    ISSN: 1573-4919
    Keywords: lymphocyte ; calcium ; glucose transporter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Generalised metabolic and electrolyte disturbances are known to accompany both plasma and surface virus infections. We have investigated whether these infections could impair the transport of Ca2+ from cells under conditions of controlled concentrations of the energy substrate glucose. Thus, cytosolic calcium levels ([Ca2+]i) were measured in single isolated lymphocytes obtained from healthy volunteers or those suffering from coryza. Before making measurements using a Ca2+-sensitive fluorescent dye indo 1, we incubated lymphocytes in buffers containing 0 mM-, 5.6 mM- or 11.2 mM-[glucose]. We found that [Ca2+]i of lymphocytes obtained from the sick were significantly higher than those from healthy controls both at 0 mM and 5.6 mM-[glucose], and that [Ca2+]i was inversely related to the media glucose concentration for both groups. These results suggest a diminished capacity of cation pumping in viral infections, such as coryza, in relationship to the available glucose as energy substrate.
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  • 6
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    Molecular and cellular biochemistry 136 (1994), S. 105-111 
    ISSN: 1573-4919
    Keywords: domoic acid ; excitotoxicity ; adenylate cyclase ; cyclic AMP ; calcium ; calmodulin ; forskolin ; Gpp(NH)p ; brain (rat)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Adenylate cyclase activity measured by the formation of cyclic AMP in rat brain membranes was inhibited by a shellfish toxin, domoic acid (DOM). The inhibition of enzyme was dependent on DOM concentration, but about 50% of enzyme activity was resistant to DOM-induced inhibition. Rat brain supernatant resulting from 105,000×g centrifugation for 60 min, stimulated adenylate cyclase activity in membranes. Domoic acid abolished the supernatant-stimulated adenylate cyclase activity. The brain supernatant contains factors which modulate adenylate cyclase activity in membranes. The stimulatory factors include calcium, calmodulin, and GTP. In view of these findings, we examined the role of calcium and calmodulin in DOM-induced inhibition of adenylate cyclase in brain membranes. Calcium stimulated adenylate cyclase activity in membranes, and further addition of calmodulin potentiated calcium-stimulated enzyme activity in a concentration dependent manner. Calmodulin also stimulated adenylate cyclase activity, but further addition of calcium did not potentiate calmodulin-stimulated enzyme activity. These results show that the rat brain membranes contain endogenous calcium and calmodulin which stimulate adenylate cyclase activity. However, calmodulin appears to be present in membranes in sub-optimal concentration for adenylate cyclase activation, whereas calcium is present at saturating concentration. Adenylate cyclase activity diminished as DOM concentration was increased, reaching a nadir at about 1 mM. Addition of calcium restored DOM-inhibited adenylate cyclase activity to the control level. Similarly, EGTA also inhibited adenylate cyclase activity in brain membranes in a concentration dependent manner, and addition of calcium restored EGTA-inhibited enzyme activity to above control level. The fact that EGTA is a specific chelator of calcium, and that DOM mimicked adenylate cyclase inhibition by EGTA, indicate that calcium mediates DOM-induced inhibition of adenylate cyclase activity in brain membranes. While DOM completely abolished the supernatant-, and Gpp (NH)p-stimulated adenylate cyclase activity, it partly blocked calmodulin-, and forskolin-stimulated adenylate cyclase activity in brain membranes. These results indicate that DOM may interact with guanine nucleotide-binding (G) protein and/or the catalytic subunit of adenylate cyclase to produce inhibition of enzyme in rat brain membranes.
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  • 7
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    Molecular and cellular biochemistry 135 (1994), S. 89-98 
    ISSN: 1573-4919
    Keywords: calcium ; cell death ; nuclei ; apoptosis ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The last decade has seen the rapid development of research investigating the molecular mechanisms whereby hormones, peptide growth factors and cytokines regulate cell metabolism, differentiation and proliferation. One general signalling mechanism used to transfer the information delivered by agonists into appropriate intracellular compartments involves the rapid Ca2+ redistribution throughout the cell, which results in transient elevations of the cytosolic free Ca2+ concentration. Ca2+ signals are required for a number of cellular processes including the activation of nuclear processes such as gene transcription and cell cycle events. The latter require that appropriate Ca2+ signals elicited in response to agonists be transduced across the nuclear envelope. It has generally been assumed that small molecules, metabolites and ions could freely diffuse across the nuclear envelope. Nevertheless several findings during the past few years have suggested that nuclear pore permeability can be regulated and that ion transport systems and ion-selective channels may exist on the nuclear membranes and regulate intranuclear processes. Intranuclear Ca2+ fluctuations can affect chromatin organization, induce gene expression and also activate cleavage of nuclear DNA by nucleases during programmed cell death or apoptosis. The possible mechanisms involved in nuclear Ca2+ transport and the control of nuclear Ca2+-dependent enzymes in apoptosis is discussed below.
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  • 8
    ISSN: 1573-4935
    Keywords: parathyroid glands ; parathyroid hormones ; calcium ; chromogranins ; hyperparathyroidism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chromogranin A is an acidic protein that is costored and cosecreted with parathyroid hormone (PTH) from parathyroid cells. Pancreastatin (PST), is derived from chromogranin A, and inhibits secretion from several endocrine/neuroendocrine tissues. Effects of different pancreastatin peptides were investigated on dispersed cells from bovine and human parathyroid glands. Bovine PST(1–47) and bovine PST(32–47) inhibited PTH release from bovine cells in a dose-dependent manner. The former peptide was more potent and suppressed the secretion at 1–100 nM. This inhibition was evident in 0.5 and 1.25 mM, but not in 3.0 mM external Ca2+. Both peptides failed to alter the concentration of cytoplasmic Ca2+([Ca2+]i) of bovine cells. Human PST(1–52) and PST(34–52) did not affect PTH release or [Ca2+]i of parathyroid cells from patients with hyperparathyroidism, nor [Ca2+]i of normal human parathyroid cells. Furthermore, bovine PST(1–47) and bovine PST(32–47) failed to alter the secretion of abnormal human parathyroid cells. The study indicates that PST exerts secretory inhibition on bovine but not human parathyroid cells, and that this action does not involve alterations of [Ca2+]i.
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  • 9
    ISSN: 1573-4935
    Keywords: Ehrlich ascites tumour cells ; energy metabolism ; methylprednisolone ; cell membrane ; phospholipid turnover ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Using Ehrlich ascites tumour cells, the short-term effects of the therapeutic glucocorticoid Methylprednisolone (MP) on the cellular energy metabolism were studied. ATP-consuming processes involved in the rapid MP effects were identified indirectly from the effects of MP on cellular oxygen consumption related to the inhibition of respiration by selective inhibitors of Ca2+-ATPase and protein synthesis. The effects of MP on plasma membrane permeability for Ca2+ ions and phospholipid turnover were studied directly by using confocal laser scanning microscopy and tracerkinetic measurements, respectively. MP inhibited cellular oxygen consumption, suppressed the inhibitory effect of lanthanum but not that of cycloheximide on oxygen consumption, blocked the [Ca2+]i rise in response to calcium ionophore A 23187, and decreased phospholipid turnover. MP acted instantly in a dose-dependent manner. The observed effects of MP are discussed in relation to the hypothesis that the drug has direct membrane effect affecting plasma membrane permeability and function.
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  • 10
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    Plant molecular biology 26 (1994), S. 1271-1287 
    ISSN: 1573-5028
    Keywords: Chlamydomonas ; calcium ; cAMP ; adenylyl cyclase ; gametogenesis ; mating response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several signal transduction pathways play important roles in the sexual life cycle of Chlamydomonas. Nitrogen deprivation, perhaps sensed as a drop in intracellular [NH4 +], triggers a signal transduction pathway that results in altered gene expression and the induction of the gametogenic pathway. Blue light triggers a second signalling cascade which also culminates in gene induction and completion of gametogenesis. New screens have uncovered several mutants in these pathways, but so far we know little about the biochemical events that transduce the environmental signals of nitrogen deprivation and blue light into the changes in gene transcription that produce gametes. Cell-cell contact of mature, complementary gametes elicits a number of responses that prepare the cells for fusion. Contact is sensed by the agglutinin-mediated cross-linking of flagellar membrane proteins. An increase in [cAMP] couples protein cross-linking to the mating responses. In C. reinhardtii the cAMP signal appears to be generated by the sequential stimulation of as many as 3 distinct adenylyl cyclase activities. Although the molecular mechanisms of adenylyl cyclase activations are poorly understood, Ca2+ may play a role. Most of the mating responses appear to be triggered by a cAMP-dependent protein kinase, but here too, Ca2+ may play a role. Numerous mutants are facilitating studies of the signalling pathways that trigger the mating responses. Cell fusion triggers another series of events that culminate in the expression of zygote specific genes. The mature zygote is sensitive to a light signal which stimulates the expression of genes whose products are essential for germination. The signal transduction pathways that trigger zygospore formation and germination are ripe for investigation in this experimentally powerful system.
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  • 11
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    The protein journal 13 (1994), S. 277-281 
    ISSN: 1573-4943
    Keywords: Fluorescence energy transfer ; zinc ; α-lactalbumin ; terbium ; cobalt ; calcium ; calcium-zinc distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract α-Lactalbumin possesses multiple Zn2+ binding sites, with the strongest site having an affinity constant of 5×105 M−1 [Permyakovet al. (1991),J. Protein Chem. 100, 577]. The binding of zinc at secondary sites is accompanied by destabilization of the protein structure and progressive protein aggregation. This pronounced destabilization is reflected in a shift of the thermal denaturation transition temperature by more than 40°. The present work examines Co2+ binding to bovineα-lactalbumin, where for this analog of Zn2+, multiple binding sites were also found from spectrofluorimetric titrations. The strong site Co2+ binding constant was 1.3×106 M−1. However, in contrast to Zn2+ binding, Co2+ does not cause protein aggregation nor any significant thermal destabilization of the protein. Fluroescence energy transfer measurements between Tb3+ in the strong calcium site to Co2+ in the strong Zn2+ site gave a distance in the range of 14–18 Å, which was in excellent agreement with recent crystallographic data for humanα-lactalbumin [Renet al. (1993), J. Biol. Chem.268, 19292–19298] However, the X-ray structure did not identify the additional zinc sites found from earlier solution studies, presumably due to restrictive crystal packing interactions. The results from the current work confirm that the strong cobalt (zinc) site in solution is the same zinc site elucidated by X-ray crystallography.
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  • 12
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    Plant molecular biology 24 (1994), S. 757-766 
    ISSN: 1573-5028
    Keywords: touch ; calcium ; indole-3-acetic acid ; salt stress ; light ; signal transduction ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata λgt 11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and MBCaM-2 are identical except for two conservative substitutions at positions 7 and 10. Southern analysis revealed that both cDNAs are encoded by different genes. Expression studies revealed different patterns of expression of both genes. MBCaM-1 mRNA exhibited a dramatic transient increase in response to touch, while MBCaM-2 expression showed a steady but small increase as compared to MBCaM-1. When plants were grown in complete darkness MBCaM-1 was undetectable and MBCaM-2 exhibited very low levels of expression. One hour after exposure of etiolated seedlings to light MBCaM-1 showed no change, while MBCaM-2 expression was increased. After a 6 h exposure to light there was an induction of both MBCaM-1 and MBCaM-2; however, the magnitude of this increase was much greater for MBCaM-2. When plants were grown under a 16 h light/8 h dark cycle the mRNA levels for MBCaM-1 were lower during the light period and increased during the beginning of the night cycle, while MBCaM-2 showed no change. Plants treated with indole-3-acetic acid had a peak in MBCaM-1 expression 6 h after treatment initiation with a slight decline 3 h after the peak, while MBCaM-2 showed a steady but small increase over time as compared to MBCaM-1. When plants were subjected to salt stress they showed an increase in MBCaM-1 expression 2 h after treatment initiation reaching a maximum after 4 h with no further increase after 6 h, while MBCaM-2 remained unchanged over the time course.
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  • 13
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    Plant molecular biology 26 (1994), S. 1529-1555 
    ISSN: 1573-5028
    Keywords: gibberellin ; growth ; development ; perception ; receptor ; gene expression ; signal transduction ; response mutant ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 14
    ISSN: 1573-4986
    Keywords: HIV ; env glycoprotein ; gp120 ; oligomerization ; glycosylation ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Envelope glycoproteins of human immunodeficiency virus (gp120 and gp41) occur as oligomers. Here, we show by gel filtration analysis that gp 120 oligomerizationin vitro is calcium- and temperature-dependent. Recombinant gp120 (rgp120) species were recovered as monomers at 20 °C in the absence of calcium, but as tetramers at 37 °C in 10mm CaCl2. Under the latter condition,N-glycanase-deglycosylated rgp120 formed hexamers. Relative to intact rgp120, which has been reported to display carbohydrate-binding properties forN-acetyl-β-d-glucosaminyl and mannosyl residues, deglycosylation enhanced rgp120 specific binding to mannose-divinylsulfone-agarose, para-aminophenyl-β-d-GlcNAc-agarose and fetuin-agarose matrices. Taken together, these results rule out the role of homologous lectin-carbohydrate interactions viaN-linked glycans in the rgp120 oligomerization, even though its lectin properties may also be calcium-dependent. Deglycosylation may unmask domains of rgp120 polypeptide backbone that independently play a role either in rgp120 lectin activity or in calcium-dependent oligomerization.
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  • 15
    ISSN: 1573-4986
    Keywords: total lipid-bound sialic acid ; LBSA ; α2,8 ketosidic linkage ; Vibrio cholerae sialidase ; cholic acid ; sodium dodecyl sulphate ; SDS ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In the measurement of total lipid-bound sialic acids involving periodic acid oxidation, as in the periodate-resorcinol assay, the inner sialic acids of disialoglycolipids (such as GD3 and GD2) are not involved because their α2,8 ketosidic linkages are resistant to periodic acid oxidation, even after acid/enzyme hydrolysis or alkali pretreatment. However, the sialic acids from these glycolipids can be recovered completely after cleavage of α2,8 linkages byV. cholerae sialidase in the presence of cholic acid, sodium dodecyl sulphate and calcium. Interestingly, removal of calcium or detergent(s) or both significantly minimizes the sialidase action on the disialyl residues of these gangliosides. Therefore, we recommend sialidase (Vibrio cholerae) pretreatment of the glycolipids in the presence of cholic acid, SDS and Ca2+ for complete recovery of sialic acids from di- and polysialogangliosides and for accurate measurement of total lipid-bound sialic acids by periodate-resorcinol assay.
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  • 16
    ISSN: 1573-904X
    Keywords: oleic acid ; alveolar epithelium ; permeability ; cellular damage ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Pulmonary exposure to oleic acid (OA) is associated with permeability alterations and cellular damage; however, the exact relationship between these two effects has not been clearly established. Using cultured alveolar epithelial monolayers, we demonstrated that OA and some other fatty acids (≤50 µM) can induce permeability changes without detectable cellular damage. At higher concentrations, however, OA caused severe membrane damage and leakage to solute flux. The permeability enhancing effect of OA was observed with both the paracellular marker 3H-mannitol and the lipophilic transcellular indicator 14C-progesterone. While the effect of OA on transcellular permeability may be attributed to its known effect on membrane fluidity, the paracellular promoting effect of OA and its mechanism are not well established. We postulated that OA may increase paracellular permeability through a Ca2+-dependent tight junction mechanism. Using dual-excitation fluorescence microscopy, we demonstrated that OA can increase intracellular calcium, [Ca2+]i , in a dose-dependent manner. This effect was transient at low OA concentrations (≤50 µM) but became more pronounced and sustained at higher concentrations. Free hydroxyl and unsaturated groups were required for this activation since esterified OA (oleic methyl ester) and stearic acid (a saturated fatty acid with equal chain length) had much reduced effects on both the [Ca2+]i and the permeability alterations. Degree of unsaturation was unimportant since linolenic acid (18:3), linoleic acid (18:2), and OA (18:1) had similar and comparable effects on the two parameters. When the alveolar epithelium was bathed with Ca2+ -free medium, OA failed to elevate [Ca2+ ]i , suggesting that Ca2+ influx from the extracellular medium is responsible for the observed [Ca2+]i rise. This effect of OA was not due to nonspecific membrane damage since the monolayer maintained its integrity and the [Ca2+]ireturned to pretreatment levels after an initial transient rise. Moreover, the permeability alteration was fully reversible upon removal of OA. These results suggest that the alveolar permeability may be reversibly enhanced by sublethal concentrations of oleic acid.
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  • 17
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    Plant and soil 163 (1994), S. 55-64 
    ISSN: 1573-5036
    Keywords: boron ; calcium ; liming ; pH ; Picea abies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of liming on concentrations of boron and other elements in Norway spruce [Picea abies (L) Karst.] needles and in the mor humus layer were studied in long-term field experiments with and without B fertilizer on podzolic soils in Finland. Liming (2000+4000 kg ha-1 last applied 12 years before sampling) decreased needle B concentrations in the four youngest needle age classes from 6–10 mg kg-1 to 5 mg kg-1. In boron fertilized plots the corresponding concentrations were 23–35 mg kg-1 in control plots and 21–29 mg kg-1 in limed plots. Both liming and B fertilizer decreased the Mn concentrations of needles. In the humus layer, total B concentration was increased by both lime and B fertilizer, and Ca and Mg concentrations and pH were still considerably higher in the limed plots than controls. Liming decreased the organic matter concentration in humus layer, whilst B fertilizer increased it. The results about B uptake were confirmed in a pot experiment, in which additionally the roles of increased soil pH and increased soil Ca concentration were separated by means of comparing the effects of CaCO3 and CaSO4. Two-year-old bare-rooted Norway spruce seedlings were grown in mor humus during the extension growth of the new shoot. The two doses of lime increased the pH of soil from 4.1 to 5.6 to 6.1, and correspondingly decreased the B concentrations in new needles from 22 to 12 to 9 mg kg-1. However, CaSO4 did not affect the pH of the soil or needle B concentrations. Hence the liming effect on boron availability in these soils appeared to be caused by the increased pH rather than increased calcium concentration.
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  • 18
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    Plant and soil 163 (1994), S. 69-75 
    ISSN: 1573-5036
    Keywords: calcium ; lime ; mycorrhiza ; pH ; Picea abies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of lime, increased soil pH and increased soil Ca concentration on the mycorrhizas of Norway spruce. [Picea abies (L.) Karst.] were studied independently of each other to elucidate the different mechanisms through which lime may influence mycorrhizas in acidic soil. In a field experiment (mature Norway spruce in podzol), lime was applied as CaCO3; increased Ca concentration without an increase in pH was achieved with CaSO4; and soil pH was increased without calcium by means of Na2CO3 and K2CO3 (Na+K treatment). Treatments were done in October, and mycorrhizas were counted from samples collected in the following June and September. All treatments increased the percentage of dead short root tips compared to controls in September, and Na+K already in June. Cenococcum geophilum Fr. increased in proportion in plots treated with Na+K. In a sand culture experiment, Norway spruce seedlings were grown from seed and inoculated with Cenococcum geophilum, or root inoculum, or left uninoculated. When mycorrhizas were beginning to form, CaCO3 and CaSO4 treatments were applied. Six weeks later, the percent of dead short root tips in both salt treatments was significantly increased from control, but formation of mycorrhizas was not inhibited by treatments. As all the treatments increased the proportion of dead short root tips, it is concluded that lime directly and adversely affected mycorrhizas of Norway spruce in sand culture and in mor humus. Both increased ionic strength and increased pH may be reasons for this rather than Ca2+ specifically.
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  • 19
    ISSN: 1573-5036
    Keywords: chlorophyll fluorescence ; fertilisation ; calcium ; magnesium ; mineral nutrition ; photosystem II ; spruce ; stomatal conductance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Severely yellowed ten-year-old spruce trees growing in the Vosges Mountains on an acidic soil were fertilised with Magnesium lime during the spring of 1990. The effects of this treatment were assessed 18 months later. A very significant improvement of the mineral status of the trees was detected, with increasing Mg contents in the needles, and as a consequence, reduced yellowing and improved chlorophyll content. Only slight differences with control trees were observed for height increase. Effects of this improved nutrition on photosynthesis were tested measuring net CO2 assimilation rates and chlorophyll a fluorescence. Light-saturated net assimilation rates of current-year needles were high, reaching 5.3 μmol m−2 s−1 on a total needle area basis. The improvement in chlorophyll and Mg content had no significant effect on net assimilation rates or on any parameter describing photochemical functions of both current-and previous-year needles. Despite the strong inter-individual variability in needle chlorophyll and Mg contents (ranging from 0.2 to 0.8 mg g−1 fresh weight, and 0.05 to 0.5 mg g-1 dry weight respectively), photochemical efficiency of PS II under limiting irradiance only decreased significantly on older needles displaying Mg contents below 0.1 mg g−1. It is concluded from these results that spruce trees exhibit a high degree of plasticity with regard to Mg deficiency on acidic soils, and that improved Mg nutrition and increased chlorophyll content do not necessarily improve photosynthesis and height growth.
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    Plant and soil 167 (1994), S. 143-148 
    ISSN: 1573-5036
    Keywords: β-naphthoxy acetic acid (NOA) ; calcium ; gibberellin ; heat ; tomato
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two cultivars of tomato with contrasting response to elevated temperature were compared: sensitive-Roma and tolerant-Robin. Experiments were done on fruit explants and on rooted cuttings with small fruits. In both cases45Ca was poorly transported to the fruits. Nevertheless in fruit explants elevated temperature (40°C) increased45Ca import into the fruits in both cultivars. In the compared cuttings, treated or not treated with growth regulators and at various temperatures, the greatest differences were observed in the amount of45Ca transported to the fruits. Sensitive Roma cuttings scantily supplied their fruits with45Ca both under optimal temperature and heat stress. In plants previously treated with NOA+GA3 high temperature increased45Ca transport to the fruits. Robin cuttings inversely responded to heat stress by transporting a much higher portion of45Ca to the fruits, both in control and NOA+GA3 cuttings. The diversity of45Ca distribution during elevated temperature in cuttings, but not in fruit explants of both cultivars seems to be connected with an ability to control calcium supply to fruit or at least to prevent its decrease; this mechanism is perhaps located outside the cluster.
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    Biogeochemistry 27 (1994), S. 1-21 
    ISSN: 1573-515X
    Keywords: calcium ; ecosystem ; forest ; oak
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    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract This study of the biogeochemical cycle of Ca has been carried out in an oak-hornbeam forest ecosystem on a soil derived from the alteration of Famennian (upper Devonian) shales. The physical nature of the geologic substrate made it possible to use the watershed-ecosystem approach by monitoring the loss of Ca by streamflow. Input of hydrogen ions and Ca from rain were average as well as that of mineral nitrogen of which 50% was ammonium-N. Modelling the biogeochemical cycle of Ca in this ecosystem proved to be difficult owing to the heterogeneity of the geologic substrate. The cationic denudation occured mostly with bicarbonate ion as a carrier of the base cations towards the streamflow. Seasonal effects on streamwater cationic composition were in relation to maximal soil respiration occuring in the autumn. In addition to this effect, the electrolyte concentration of streamwater was determined by rain and by changes in concentration of the soil solution either by evapotranspiration or by freezing. In spite of widely fluctuating values of flow rate, the concentration of the streamflow remained constant within a factor of two or at the most three. The mechanism underlying this buffering action of the soil on the concentration of the solution flowing through it, was shown to be related to ion-exchange processes.
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  • 22
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    Fish physiology and biochemistry 12 (1994), S. 479-484 
    ISSN: 1573-5168
    Keywords: Notothenioidei ; calcium ; bone ; efflux ; plasma ; vitamin D3 ; 25-(OH)-D3 ; 1,25-(OH)2-D3
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    Topics: Biology
    Notes: Abstract The Antarctic notothenioid, Pagothenia bernacchii, were found to have plasma total and free calcium levels, plasma inorganic phosphate and whole body calcium efflux rates which were similar to those seen in other teleosts. But total bone calcium was lower than reported for other teleosts. A single injection of vitamin D3 (5 ng g−1 fish) increased plasma total and plasma free calcium and these increases were associated with an increase in whole body calcium efflux and bone calcification. Conversely, the same treatment with 1,25-(OH)2-D3 reduced plasma free calcium. This seco-steroid also increased the specific activity of 45Ca in bone at 40h post-injection but did not significantly effect total bone calcium, plasma total calcium or whole body calcium efflux. 25-OH-D3 at the same dose had no effect on any of the parameters tested and none of the seco-steroids tested had any effect on plasma total inorganic phosphate. These data show that both D3 and 1,25-(OH)3-D3 can have calcitropic effects in this marine teleost and that these two forms of vitamin D can exert different effects within the same species.
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  • 23
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    Journal of bioenergetics and biomembranes 26 (1994), S. 471-485 
    ISSN: 1573-6881
    Keywords: Mitochondria ; transport ; calcium ; metabolic mediator ; kinetics ; calcium pulses
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    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The identification of intramitochondrial free calcium ([Ca2+ m) as a primary metabolic mediator [see Hansford (this volume) and Gunter, T. E., Gunter, K. K., Sheu, S.-S., and Gavin, C. E. (1994)Am. J. Physiol. 267, C313–C339, for reviews] has emphasized the importance of understanding the characteristics of those mechanisms that control [Ca2+]m. In this review, we attempt to update the descriptions of the mechanisms that mediate the transport of Ca2+ across the mitochondrial inner membrane, emphasizing the energetics of each mechanism. New concepts within this field are reviewed and some older concepts are discussed more completely than in earlier reviews. The mathematical forms of the membrane potential dependence and concentration dependence of the uniporter are interpolated in such a way as to display the convenience of consideringV max to be an explicit function of the membrane potential. Recent evidence for a transient rapid conductance state of the uniporter is discussed. New evidence concerning the energetics and stoichiometries of both Na+-dependent and Na+-independent efflux mechanisms is reviewed. Explicit mathematical expressions are used to describe the energetics of the system and the kinetics of transport via each Ca2+ transport mechanism.
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  • 24
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    Plant growth regulation 14 (1994), S. 235-242 
    ISSN: 1573-5087
    Keywords: auxin transport ; calcium ; gravitropism ; root cap ; Zea mays
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract There is evidence that the cap is the initial site of lateral auxin redistribution during the gravitropic response of roots. We tested this further by comparing asymmetric auxin redistribution across the tips of gravistimulated intact roots, decapped roots, isolated root caps and isolated apical sections taken from decapped roots. Gravistimulation caused asymmetric (downward) auxin movement across the tips of intact roots and isolated root caps but not across the tips of decapped roots or across isolated apical root segments. Naphthylphthalamic acid and pyrenoylbenzoic acid, inhibitors of polar auxin transport, inhibited asymmetric auxin redistribution across gravistimulated isolated root caps and across the tips of gravistimulated intact roots. For intact roots there was a positive correlation between the extent of inhibition of assymmetric auxin redistribution by polar auxin transport inhibitors and the extent of inhibition of asymmetric calcium chelating agent, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, also caused parallel inhibition of asymmetric auxin redistribution and gravitropic curvature and this effect was reversed by subsequent treatment with calcium. The results support the hypothesis that the cap is a site of early development of auxin asymmetry in gravistimulated roots and that calcium plays an important role in the development of lateral auxin redistribution.
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  • 25
    ISSN: 1573-6822
    Keywords: calcium ; dihydropyridine ; lactotroph ; TRH
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    Topics: Biology , Medicine
    Notes: Abstract Prolactin (PRL) release and intracellular free calcium concentration [Ca2+]i were measured in two populations of normal rat lactotrophs (light and heavy fractions) in culture. Spontaneous PRL release of heavy fraction cells was more sensitive to dihydropyridines (DHPs; Bay K 8644 and nifedipine) when compared to the light fraction lactotrophs. The stimulatory effect of thyrotropin-releasing hormone (TRH) on PRL release from heavy fraction cells was inhibited by Cd2+ and mimicked by Bay K 8644. Indo-1 experiments revealed that TRH-increased [Ca2+]i was reversibly inhibited by Cd2+. In a Ca2+-free EGTA-containing medium, TRH did not modify [Ca2+]i.
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  • 26
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    Cell biology and toxicology 10 (1994), S. 317-322 
    ISSN: 1573-6822
    Keywords: bradykinin ; calcium ; ionomycin ; scanning cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ligand-receptor affinity is classically demonstrated by measuring ligand binding density to a specific site on membrane preparations, and receptor function is studied by measuring calcium flux, cell by cell, using microspectrofluorimetry. In order to study these phenomena in a larger cell population, calcium flux was measured in MRC-5 cell line expressing the B2 receptor for bradykinin using an ACAS 570 scanning cytometer. Following incorporation of fluo3/AM, different ligands were studied, singly or in association with bradykinin. This study confirmed that only the B2 receptor is present on the plasma membrane of MRC-5 cells. Bradykinin binding to the B2 receptor was not modified by a B1 agonist (Des-Arg9-bradykinin) or by a B1 antagonist (Des-Arg9-[Leu8]-bradykinin) but was inhibited by a B2 agonist ([Hyp3]-bradykinin) and a B2 antagonist (HOE 140). The source of free calcium was also studied in comparison with ionomycin. The intensity of the calcium peak after binding of bradykinin is independent of the concentration of extracellular calcium. Preincubation with diltiazem or TMB-8 did not modify calcium flux indicating that transduction of the signal after bradykinin binding in this cell line is independent of voltage-dependent channels and does not require mobilization of intracellular calcium blocked by TMB-8. In conclusion, scanning cytometry can be used to study ligand-receptor binding and to obtain results rapidly from multiple cells. Recording of individual cell variations and kinetics enables identification of active agonists or antagonists and consequently the selection of new compounds.
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  • 27
    ISSN: 1573-6822
    Keywords: BHK21 C13 ; calcium ; cell division cycle ; flow cytometry ; FR 3T3 ; scanning laser cytometry
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    Topics: Biology , Medicine
    Notes: Abstract Maitotoxin (MTX) induces an increase of [Ca2+]i and of phosphoinositide breakdown in various cell types. The [Ca2+]i increase followed with fluorescent probes on cell suspensions has been described as slow and lasting, in contrast to the “signal” induced by calcium ionophores such as ionomycin. MTX effects have been studied on two fibroblastic cell lines, BHK21 C13 and FR 3T3, synchronized by serum deprivation treatment performed in an isoleucine-free medium for BHK21 C13 cells. In BHK21 C13 cells, flow cytometry analysis showed that two stages, G1/S and G2/M, were particularly susceptible to MTX treatment. Scanning laser cytometry demonstrated that calcium response of FR 3T3 fibroblasts followed with Indo-1 varied during the cell division cycle. The [Ca2+]i increase was almost always vertical, but its delay after MTX addition lasted from zero (S and G2/M transition) to 10–20 min (G1) or more (G2). No [Ca2+]i change could be detected during mitosis. The [Ca2+]i response at the S phase was biphasic. These observations suggest that (1) the lasting response described in the literature represents a global cell population effect, and (2) cells are more sensitive to MTX at specific stages of the cell division cycle, which could correspond to periods when calcium signals have been detected in different cell types.
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  • 28
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    Magnetic resonance materials in physics, biology and medicine 2 (1994), S. 433-435 
    ISSN: 1352-8661
    Keywords: MR spectroscopy ; 19F-MR spectroscopy ; calcium ; zinc ; glutamate ; NMDA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics
    Notes: Abstract The19F-NMR calcium indicator 5FBAPTA has been used to measure changes in free intracellular calcium, [Ca2+],, in superfused guinea pig cerebral cortical slices. Increases in [Ca2+], have been observed with depolarization and the combined insult of hypoxia and hypoglycemia. These increases in [Ca2+]i, can be explained by the excitotoxic hypothesis which proposes a central role for the neurotransmitter glutamate and the NMDA receptor in cell death. We, therefore, investigated the effects of glutamate and NMDA directly on [Ca2+]i, in cerebral cortical slices with31P-NMR to monitor the energy state. In the presence of glutamate (±Mg) a new resonance was observed in the19F spectra and was attributed to the Zn-5FBAPTA complex. The zinc peak appears with or just following an increase in [Ca2+]i, and with a drop in PCr. Similar results were obtained on exposing the tissues to NMDA. Zinc has been reported to be enriched in various parts of the brain and have a multiplicity of possible roles. This is the first time zinc has been shown in actively metabolizing tissue. The observation that the zinc resonance does not appear with depolarization or hypoxia and hypoglycemia suggests that the mechanisms of damage in these latter insults are not solely attributable to the release of excitotoxins.
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  • 29
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    Nutrient cycling in agroecosystems 35 (1993), S. 217-226 
    ISSN: 1573-0867
    Keywords: calcium ; dry matter distribution ; fertilizer ; harvest index ; magnesium ; manure ; millet ; nitrogen ; nutrient uptake ; phosphorus ; potassium ; Senegal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a fertilizer and manure experiment, millet was grown under four treatments (no fertilizer or manure, farmyard manure, chemical fertilizer, and both). Grain yield and total aboveground biomass production of the unfertilized plot were relatively high. The observed differences in total dry matter production must be attributed to differences in nutrient availability, as amount of rainfall and its distribution were favourable. Results show only small differences in distribution of dry matter among the various plant organs between the best and the non-fertilized treatments. Nutrient supply from natural sources, defined as crop content of N, P, and K at maturity without fertilizer application, amounted to 104, 16 and 103 kg ha−1, respectively, which are very high values. Total uptake of calcium and magnesium is related to that of potassium, as the combined content of these three elements is linearly related to total aboveground biomass production. Minimum removal of nitrogen and phosphorus per ton grain dry matter amounts to 29 and 4kg, respectively, and 9 kg potassium per ton total aboveground dry matter. A possible double function of phosphorus as element of structural biomass and for maintenance of electro-neutrality is discussed.
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    Cellular and molecular life sciences 49 (1993), S. 865-869 
    ISSN: 1420-9071
    Keywords: Zinc ions ; calcium ; ileal muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Preincubation with 0.3 mM Zn2+ markedly inhibited both the tonic response and Ca2+ binding at low affinity sites induced by K+ (60 mM), with smaller effects on the phasic response and the high affinity Ca2+ sites, inTaenia coli. However, when the muscle was kept in Zn2+-containing medium following the first stimulation with the K+, the phasic response and the high affinity Ca2+ sites were more severely inhibited during the second stimulation with K+. This probably indicates that Zn2+ reduced the tonic tension response to K+ mainly by inhibiting Ca2+ influx at the cell membranes ofTaenia coli. However, when Zn2+ is continuously present, Ca2+ is not supplied at the storage sites and is not available for the phasic response to a second stimulation with K+.
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    Cellular and molecular life sciences 49 (1993), S. 1064-1072 
    ISSN: 1420-9071
    Keywords: Aminooxyacetic acid ; 1-methyl-4-phenylpyridinium ion ; malonic acid ; 3-nitropropionic acid ; rotenone ; sodium azide ; nitric oxide ; N-methyl-D-aspartate ; oxidative phosphorylation ; calcium ; cell death
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract It is thought that impairment, of energy metabolism that results in deterioration of membrane function, leading to loss of the Mg2+ block on NMDA receptors, and allowing persistent activation of these receptors by glutamate, might be a cause of neuronal death in neurodegenerative disorders. Studies in rodents using mitochondrial respiratory chain toxins, such as aminooxyacetic acid, 1-methyl-4-phenylpyridinium, malonic acid and 3-nitropropionic acid, suggest that such processes may indeed be involved in neurotoxicity. Striatal and nigral degeneration induced by mitochondrial toxins in rodents resembles the neuropathology seen in humans suffering from Huntington's or Parkinson's disease, and can be prevented either by decortication or by NMDA receptor antagonists. Such experimental observations suggest that glutamate may be involved in neuronal death leading to neurodegenerative disorders in humans. If so, glutamate antagonists may offer a therapeutic approach for retarding the progression of these disabling disorders.
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  • 32
    ISSN: 1573-4919
    Keywords: mucosa ; membranes ; smooth muscle ; calcium ; adenosine triphosphatase ; arteries ; myocardium
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Heart tissue contains large amounts of the protein encoded by the Ca2+ pump gene SERCA2. The SERCA2 RNA can be spliced alternatively to produce mRNA encoding the proteins SERCA2a and SERCA2b which differ in their C-terminal sequences. In this study we report the tissue distribution of SERCA2a and SERCA2b isoforms byin situ hybridization to rabbit heart and stomach. The expression of SERCA2 mRNA was high in myocardial cells, being the highest in the atrial region. In contrast, there was more SERCA2 protein in Western blots in ventricles than in atria. Myocardial cells expressed predominantly the mRNA for the isoform SERCA2a. Whereas the stomach smooth muscle and the neuronal plexus expressed SERCA2 at levels much lower than myocardial cells, the expression was very high in the stomach mucosa. Mucosa contained mainly the mRNA for SERCA2b. From immunocytochemistry it was concluded that the anti-heart SR Ca2+ pump antibody IID8 reacted much better with heart and surface mucosal cells in the stomach than with the stomach smooth muscle, and that IID8 reactivity was intracellular. In contrast PM4A2B, an antibody against the plasma membrane Ca2+ pump, reacted well with heart and stomach smooth muscle, plexus and mucosa, and its localization appeared to be in the plasma membrane. Thus, stomach smooth muscle expressed SERCA2b mRNA and protein at low levels, mucosa expressed SERCA2b mRNA and protein at high levels, atria and ventricle expressed SERCA2a mRNA and protein at high levels, mRNA being more in atria, but protein being more in ventricles.
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    Molecular and cellular biochemistry 127-128 (1993), S. 229-237 
    ISSN: 1573-4919
    Keywords: calcium ; contraction ; smooth muscle ; myosin ; protein phosphorylation ; second messengers
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Phosphorylation of the regulatory light chain of myosin by the Ca2+/calmodulin-dependent myosin light chain kinase plays an important role in smooth muscle contraction, nonmuscle cell shape changes, platelet contraction, secretion, and other cellular processes. Smooth muscle myosin light chain kinase is also phosphorylated, and recent results from experiments designed to satisfy the criteria of Krebs and Beavo for establishing the physiological significance of enzyme phosphorylation have provided insights into the cellular regulation and function of this phosphorylation in smooth muscle. The multifunctional Ca2+/calmodulin-dependent protein kinase II phosphorylates myosin light chain kinase at a regulatory site near the calmodulin-binding domain. This phosphorylation increases the concentration of Ca2+/calmodulin required for activation and hence increases the Ca2+ concentrations required for myosin light chain kinase activity in cells. However, the concentration of cytosolic Ca2+ required to effect myosin light chain kinase phosphorylation is greater than that required for myosin light chain phosphorylation. Phosphorylation of myosin light chain kinase is only one of a number of mechanisms used by the cell to down regulate the Ca2+ signal in smooth muscle. Since both smooth and nonmuscle cells express the same form of myosin light chain kinase, this phosphorylation may play a regulatory role in cellular processes that are dependent on myosin light chain phosphorylation.
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    The journal of membrane biology 131 (1993), S. 237-243 
    ISSN: 1432-1424
    Keywords: olfaction ; receptor neuron ; cilia ; cyclic-nucleotide-gated channel ; calcium ; magnesium
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Olfactory receptor neurons depolarize in response to odorants. This depolarization is mediated by an increase in intracellular cyclic AMP, which directly gates channels in the membranes of the neuronal cilia. Previous evidence suggests that a Ca2+ influx during the odorant response may ultimately play a role in terminating the response. One way Ca2+ inside the cell could terminate the odorant response would be to directly inhibit the cAMP-gated channels. In this report the effects of cytoplasmic Ca2+ and Mg2+ on the cAMP-activated current were measured in single olfactory cilia. Near the neuronal resting potential, cytoplasmic Ca2+ and Mg2+ only slightly reduced the cAMP-activated current. Even at high levels (1.0mm Ca2+ or 5.0mm Mg2+), the average inhibition was only around 20%. It is therefore unlikely that an influx of divalent cations terminates the odorant response by a direct effect on the cAMP-gated channels.
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    The journal of membrane biology 132 (1993), S. 201-209 
    ISSN: 1432-1424
    Keywords: keratinocyte ; patch clamp ; involucrin ; ion channels ; calcium
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary In vivo and in vitro, keratinocyte differentiation is linked with increased extracellular Ca2+. In order to correlate ion channels with cell differentiation and investigate keratinocyte membrane responses to Ca2+, keratinocyte single channel currents were studied using the patch-clamp technique. The most frequently observed channel was a 14 pS nonspecific cation channel. This channel was permeable to Ca2+ and activated by physiological concentrations of Ca2+. We also found a 35 pS Cl− channel whose open probability increased with depolarization. Finally, a 70 pS K+ channel was seen only in cell-attached or nystatin-permeabilized patches. We correlated channel types with staining for involucrin, an early marker of keratinocyte differentiation. While the nonspecific cation channel and Cl− channel were seen in both involucrin positive and involucrin negative cells, all channels in which the K+ channel activity was present were involucrin positive. Membrane currents through these channels may be one pathway by which signals for keratinocyte proliferation or differentiation are sent.
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  • 36
    ISSN: 1573-5176
    Keywords: seaweed ; Cytoseira ; heavy metal combination ; experimental design ; toxicity ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The toxicity of three heavy metals, Cd, Cu and Zn, and the detoxifying role of Ca have been studied for the brown algaCystoseira barbata formaaurentia after a 4-week laboratory culture. The experimental design was based upon a complete factorial design 2k, which seems to be the first time it has been used in algal physiology. It was demonstrated that these three elements, applied jointly, act on weight-growth, chlorophyll a, c and carotenoid synthesis and Cd, Cu and Zn uptake. Cd and Zn act in synergy or in antagony, depending on their exogenous concentrations, on chlorophyll a and on carotenoid synthesis. Zn is antagonistic towards Cd and Cu on weight-growth in the combination Cd-Cu-Zn. From different element combinations, the protective role of Ca appears evident on weight-growth (Cd-Zn-Ca and Cu-Ca), chlorophyll a (Cd-Cu-Ca and Cu-Zn-Ca), chlorophyll c (Cd-Ca), carotenoid synthesis (Cd-Cu-Ca and Cu-Zn-Ca), Cd and Cu uptake (Cd-Cu-Ca) and Zn uptake (Cu-Zn-Ca). This role is confirmed by cytological investigations. This is apparently the first report concerning a Ca interaction with toxicity of heavy metals applied in combinations. However, the mechanisms of tolerance remain unknown.
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  • 37
    ISSN: 1573-904X
    Keywords: alveolar epithelium ; calcium ; cell culture ; enkephalins ; epithelial transport ; peptide hydrolysis ; pulmonary absorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract An in vitro cultured monolayer system of alveolar epithelial cells was used as a model to investigate transport and hydrolysis of two enkephalin peptides, Met-enkephalin (TGGPM) and [D-Ala2]Met-enkephalinamide (TAGPM), in pulmonary epithelium. Isolated alveolar type II cells formed continuous monolayers when grown on microporous tissue culture-treated polycarbonate filters in serum-free, hormonally defined medium. Transport and hydrolysis studies of enkephalins in the monolayer system obtained after 6 days in culture, using fluorescence reversed-phase HPLC, indicate a reduced but significant degradation of enkephalins in the alveolar epithelium compared to most other epithelia previously reported. Aminopeptidases and dipeptidyl carboxypeptidase represent two major hydrolytic enzymes for TGGPM, as indicated by the formation of the degradative products Tyr and Tyr-Gly-Gly, while dipeptidyl peptidase, which is responsible for the formation of Tyr-Gly, contributes much less. The enkephalinase inhibitor thiorphan failed to prevent the hydrolysis of TGGPM whereas the enkephalin analog TAGPM was relatively resistant to enzymatic cleavage. The rate of enkephalin transport across the alveolar epithelium was directly proportional to drug concentration and occurred irrespective of transport direction, suggesting passive diffusion as the major mechanism for transepithelial transport. Agents that affect paracellular transport pathways, e.g., EGTA and the calcium ionophore A-23187, greatly promoted the transport rate. The ionophore at high doses, in addition to promoting tight junction permeability, also caused cellular damage associated with a sustained rise in intracellular calcium levels, as indicated by nuclear propidium iodide fluorescence. The cultured monolayer of alveolar epithelium may be used to study pulmonary drug absorption, degradation, and toxicity.
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  • 38
    ISSN: 1573-904X
    Keywords: tracheal epithelium ; paracellular ; tight junction permeability ; calcium ; cytoskeleton
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The present study investigates the mechanisms controlling tight junction permeability of the tracheal epithelium, with an emphasis on the regulatory role of intra- and extracellular calcium as well as the cell cytoskeleton. The tracheas were isolated from rabbits and their junctional permeability barrier was investigated in vitro by means of transepithelial electrical resistance measurements and flux measurements of the radiolabeled paracellular tracer, 14C-mannitol. The effects of intra- and extracellular calcium were studied using the calcium ionophore A 23187 and EGTA, and that of the cytoskeleton was investigated using cytochalasin B. Intracellular calcium of the tracheal epithelium was monitored microfluorometrically using the specific calcium indicator, Fura-2 AM (acetoxymethyl ester). The results indicate that the tight junction permeability of the trachea was significantly increased upon treatment with all three of the test compounds, as evidenced by a substantial decrease in transepithelial electrical resistance and an increase in transepithelial flux of 14C-mannitol. The effects of EGTA and cytochalasin B on the tight junction permeability are fully reversible upon removal of the compounds from the bathing media. On the other hand, tissues treated with the calcium ionophore demonstrate a partial or no recovery in membrane permeability, depending on the intracellular calcium levels. Moderate and transient increases in intracellular calcium caused a partial reversibility of the membrane resistance, while high and sustained intracellular calcium levels induce a complete irreversibility of the membrane resistance. These results suggest that high extracellular calcium levels and low intracellular calcium levels are required for the normal maintenance of the junctional permeability in the tracheal epithelium. Studies using cytochalasin B indicate that there is also a close relationship between the tight junctions and the organization of actin microfilaments. Alterations of these structures as well as cellular calcium levels can result in a substantial change in transepithelial permeability. Therefore compounds that affect tight junction permeability may exert their action through the calcium and cytoskeleton mechanisms.
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    Plant and soil 149 (1993), S. 87-94 
    ISSN: 1573-5036
    Keywords: aluminium ; calcium ; calcium pectate ; polygalacturonic acid ; solution pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Extracellular processes, particularly the adsorption of aluminium (Al) by pectate in the cell wall, have been proposed as important in the expression of Al toxicity to plant roots. In vitro studies were conducted on the effects of Al concentration (generally ≤ 32 μM), calcium (Ca) concentration (0.05 to 10 mM) and pH (3.2 to 5.4) on Al sorption by Ca pectate. There was a rapid reaction between Al and Ca pectate, there being no difference in Al remaining in solution after reaction times of 1 to 16 min, and only a slight decrease after 24 h. Increased Al concentration in solution increased linearly the sorption of Al by Ca pectate, with 70 to 84% of the Al originally in solution sorbed with ≤32 μM Al. In contrast, Al sorption decreased with increased Ca concentration in solution, and as pH decreased from 5.4 to 3.2. Only ≤30% of the sorbed Al was desorbed after 1 h by 1 mM CaCl2, 10 mM CaCl2 or 1 mM HCl. The amount of Al desorbed increased with a desorption period of 5 h, particularly with 1 mM HCl. These studies suggest that Al sorbed by Ca pectate in root cell walls is in equilibrium with Al in solution, and that Al toxicity is associated with the strong binding between Al and Ca pectate external to the cytoplasm.
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  • 40
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    Plant and soil 155-156 (1993), S. 415-418 
    ISSN: 1573-5036
    Keywords: Brassica ; calcium ; growth ; salinity ; salt tolerance ; seawater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Whole plant and callus cultures of different rapid-cycling Brassica species were treated with salinity (8 dS m-1) and/or supplemental Ca (up to 10 mM total concentration). None of these cultures responded to supplemental Ca with improved growth indicating that the salt tolerance of these genotypes was not dependent upon Ca.
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  • 41
    ISSN: 1573-5036
    Keywords: aluminium ; calcium ; dicotyledons ; magnesium ; monocotyledons ; nutrition ; specific root length
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Growth inhibition of plants suffering from Al toxicity is generally accompanied by impaired root development which can be quantitatively described by reduced specific root length (m g-1 dry root). In addition, the uptake of nutrients such as Mg and Ca is inhibited. Increased supply of either Mg or Ca can significantly diminish the negative effect of Al on root development and improve the Mg or Ca nutrition of the plants. The positive effect of Ca is well established but the effect of Mg has been observed in only a few plan† species. Therefore, the effects of increasing Mg and Ca supply on Al toxicity in plants of seven monocots and eight dicots have been now examined in nutrient solution experiments. In general, Mg appears to be more effective than Ca in alleviating Al toxicity with the monocots, whereas the reverse is true for the dicots. Increased concentrations of Mg and Ca in solution seem to protect the plants against Al toxicity by improving the Mg or Ca nutrition and by alleviating the toxic effect of Al on root development.
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  • 42
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    Plant and soil 149 (1993), S. 197-203 
    ISSN: 1573-5036
    Keywords: aluminum-toxicity ; calcium ; Glomus aggregatum ; Leucaena leucocephala ; lime ; pinnule P content ; soil acidity ; soil solution ; ultisol ; VAM colonization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A greenhouse investigation was undertaken to determine the influence of fresh organic matter on the formation and functioning of vesicular-arbuscular mycorrhizal symbiosis in Leucaena leucocephala grown in an acid aluminum-rich ultisol. In soil not amended with fresh organic matter or lime, plants failed to grow. Mycorrhizal infection level, mycorrhizal effectiveness measured in terms of pinnule P content of L. leucocephala leaves and dry matter yield of the legume increased with increase in fresh organic matter. Although VAM colonization level and dry matter yield of L. leucocephala were significantly higher if the test soil was limed (7.2 cmole OH−) than if amended with fresh organic matter, the latter was as effective as lime in off-setting the detrimental effect of aluminum on mycorrhizal effectiveness. The lower mycorrhizal colonization level and the lower dry matter yield noted in the soil treated with fresh organic matter appears to be related to the inadequacy of Ca in the soil amended with fresh organic matter. These observations are supported by the low calcium status of soil and plant tissues in the absence of lime. It is concluded that while fresh organic matter, in appropriate amounts, could protect sensitive plants and VAM symbiosis against Al toxicity in acid soils, maximum mycorrhizal inoculation effects are not likely to be attained unless the soils are also amended with Ca.
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  • 43
    ISSN: 1573-5036
    Keywords: aluminium ; ammonium ; Ca-Ionophore A 23187 ; calcium ; cytoplasmic streaming ; root hairs ; Triticum aestivum (L.)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The calcium dependency of the cytoplasmic streaming of wheat root hairs was demonstrated by adding the Ca-Ionophore A 23187. Within three minutes the streaming velocity was decreased dramactically. The influence of ammonium on the cytoplasmic streaming is highly pH-dependent. While at a pH of 9.0 an inhibitory effect was observed even at low ammonium concentrations (0.5 mM) no effect could be measured at a pH of 6.5. Nitrate, independently of medium pH had no effect on the cytoplasmic streaming. The same is true for aluminium. It is suggested that at pH 9 ammonium permiates the plasmalemma as NH3. Due to higher cytoplasmic pH (∼ 7.5), NH3 is protonated leading to an increase in cytoplasmic pH. Ammonium may displace sorbed calcium leading to an increase in the free cytoplasmic calcium responsible for the cessation of the streaming. Alternative explanations are discussed.
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  • 44
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    Plant cell, tissue and organ culture 33 (1993), S. 331-338 
    ISSN: 1573-5044
    Keywords: calcium ; genotype ; growth regulator ; macronutrient ; rubber tree ; sucrose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of plant growth regulators, sucrose, calcium and various macronutrient media on callus friability and somatic embryogenesis was investigated inHevea brasiliensis Müll. Arg. Friable and embryogenic calli were spontaneously formed in two rubber tree clones (PR 107 and RRIM 600) on the Medium for Hevea (MH), with 3,4-dichlorophenoxyacetic acid (3,4-d), kinetin and sucrose, while compact embryogenic calli were enhanced in three other clones (PB 260, PB 235 and GT1). Callus friability was enhanced in clone PB 260 when the concentration of one growth factor (3,4-d or kinetin) was reduced from 4.5 μLM to 0.45 μM during the first culture, or when high sucrose or calcium levels 351 mM and 12 mM, respectively) were maintained during subcultures. The different macronutrient media did not alter callus texture but only use of MH and Murashige and Skoog (MS) media led to somatic embryogenesis. Friable calli obtained by modifying the auxin/cytokinin balance lost their embryogenic potential. In contrast, those obtained on media with high sucrose or calcium concentrations were mainly composed of embryogenic cells embedded in a mucilaginous matrix. Such calli could be of potential interest for establishing embryogenic cell suspension cultures.
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  • 45
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    Photosynthesis research 37 (1993), S. 117-130 
    ISSN: 1573-5079
    Keywords: Photosystem II ; calcium ; oxygen evolution ; primary quinone acceptor ; redox potential ; fluorescence quenching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract pH-dependent inactivation of Photosystem (PS) II and related quenching of chlorophyll-a-fluorescence have been investigated in isolated thylakoids and PS II-particles and related to calcium release at the donor side of PS II. The capacity of oxygen evolution (measured under light saturation) decreases when the ΔpH is high and the pH in the thylakoid lumen decreases below 5.5. Oxygen evolution recovers upon uncoupling. The pH-response of inactivation can be described by a 1 H+-transition with an apparent pK-value of about 4.7. The yield of variable fluorescence decreases in parallel to the inactivation of oxygen evolution. pH-dependent quenching requires light and can be inhibited by DCMU. In PS II-particles, inactivation is accompanied by a reversible release of Ca2+-ions (one Ca2+ released per 200 Chl). In isolated thylakoids, where a ΔpH was created by ATP-hydrolysis, both inactivation of oxygen evolution (and related fluorescence quenching) by internal acidification and the recovery of that inactivation can be suppressed by calcium-channel blockers. In the presence of the Ca2+-ionophore A23187, recovery of Chl-fluorescence (after relaxation of the ΔpH) is stimulated by external Ca2+ and retarded by EGTA. As shown previously (Krieger and Weis 1993), inactivation of oxygen evolution at low pH is accompanied by an upward shift of the midpoint redox-potential, Em, of QA. Here, we show that in isolated PS II particles the pH-dependent redox-shift (about 160 mV, as measured from redox titration of Chl-fluorescence) is suppressed by Ca2+-channel blockers and DCMU. When a redox potential of −80 to −120mV was established in a suspension of isolated thylakoids, the primary quinone acceptor, QA, was largely reduced in presence of a ΔpH (created by ATP-hydrolysis) but oxidized in presence of an uncoupler. Ca2+-binding at the lumen side seems to control redox processes at the lumen- and stroma-side of PS II. We discuss Ca2+-release to be involved in the physiological process of ‘high energy quenching’.
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  • 46
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    Plant and soil 153 (1993), S. 19-31 
    ISSN: 1573-5036
    Keywords: Brassica carinata ; Brassica napus ; calcium ; chloride ; growth analysis ; leaf area ratio ; magnesium ; net assimilation rate ; potassium ; relative growth rate ; seawater ; sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The response of two rapid-cycling Brassica species differing in tolerance to seawater salinity was studied over a period of 24 days. In response to 8 dS m−1 salinity, the two Brassica species showed clear differences in the changes in relative growth rate (RGR), net assimilation rate (NAR) and leaf area ratio (LAR). The RGR of B. napus was slightly reduced by salinity, wheareas the RGR of B. carinata was largely reduced in the early stages of salinization. LAR of B. napus was affected by salinity in the later stages of growth and significantly correlated with the reduction in RGR. On the other hand, the NAR of B. carinata was decreased by salinity, corresponding to the decrease of the RGR of B. carinata. The NAR of B. napus was not significantly affected by salinity according to analysis of covariance. The shoot concentrations of Na, Mg and Cl increased while the concentrations of K and Ca decreased sharply during the first 5 days of salinization; subsequently, all ion concentrations remained relatively constant. The concentrations of Na, K, Ca, Mg and Cl in the root were similarly affected by salinity. There were no significant differences of ion concentrations between species that could be related to the differences in salt tolerance. Thus, the differences in salt tolerance between species can not be related to differences in specific-ion effects, but may be related to some factor that reduces the NAR of B. carinata during the early stages of growth.
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  • 47
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    Plant and soil 153 (1993), S. 281-285 
    ISSN: 1573-5036
    Keywords: calcium ; lithium metaborate fusion ; magnesium ; plant tissue ; potassium ; silicon ; wild rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A rapid batch method was developed for the analysis of Si, Ca, Mg, and K in a large number of plant tissue samples by fusion with lithium metaborate (LiBO3) in graphite crucibles with the use of a molybdenum blue spectrophotometric analysis of silicon and FAAS for Ca, Mg, and K. Our method was tested for whole plant analysis of mature wild rice (Zizania palustris L.). Analysis of Si in plant tissue with LiBO3 in graphite crucibles is reliable and fast. Thirty-six samples can be ashed overnight, fused in one hour the next day, and analyzed for Si within approximately two hours.
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  • 48
    ISSN: 1573-5036
    Keywords: calcium ; copper ; iron ; lysine ; magnesium ; manganese ; methionine ; nutrition ; nutritional quality ; seed vigor ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of the maize genes, o 2 and Mal, on the concentrations of mineral nutrient cations and amino acid levels in mature maize (Zea mays L) kernels of various inbred lines were studied. Previously, the o 2 gene has been used to improve the protein quality and increase the mineral nutrient content of kernels from some inbred lines. Genotypes possessing the Mal (multiple aleurone layer) gene, contain more than one row of aleurone cells in their kernels and this gene enhances the effect of the o 2gene on improving kernel protein quality. Incorporating these genes into the maize genome increased accumulation of several mineral nutrients (including Ca, Mg, Zn, Fe, Mn, Zn and Cu) in some of the experimental lines studied. The physiological basis for this increase of mineral nutrients in the kernels is discussed. The effect of the Mal gene on the kernel amino acid composition and protein quality was also examined. Possibly, these genes could be used in combination in breeding programs to improve kernel quality and nutritional value of maize.
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  • 49
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    Plant and soil 148 (1993), S. 107-113 
    ISSN: 1573-5036
    Keywords: calcium ; rice ; silica body ; silicon ; soft X-ray irradiation
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The interaction between Ca and Si in water-cultured rice plants (Oryza sativa L. cv. Akebono) was investigated in terms of uptake. The effect of Ca levels in the solution on Si chemical forms and on the formation of silica bodies in the leaf blades was also examined using soft X-ray irradiation for detection of silica bodies. Si addition (1.66 mM Si) decreased both Ca content of the shoot and uptake at each Ca level. This might mainly result from a decreased transpiration rate caused by Si. Si uptake was not affected when the Ca levels were increased. The results of Si forms showed that silica sol constituted more than 90% of the total Si in the leaf blades regardless of Si and Ca levels, and soluble silica and/or polysilicic acid seems to gel physically over 8.0 mM Si within the plants. Significant difference in the numbers of silica bodies on the third leaf blade was not found between different Ca levels at the same Si level. The content of Si in the leaf blade seems to be a determining factor for the formation of silica bodies. ei]H. Marschner
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  • 50
    ISSN: 1573-5036
    Keywords: apple orchard ; boron ; calcium ; magnesium ; mineralogy ; spent bed ash ; sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Currently, there is renewed interest in the agricultural utilization of coal combustion byproducts. Field sites where high rates (112 Mg ha−1) of high gypsum coal combustion spent bed ashes were surface applied in 1980 within fruit tree orchard rows were identified and sampled with depth. The objective of this study was to examine the effects on long-term exposure/leaching of these materials on soil profile chemical properties. When applied, the material had an aqueous pH of 12.5 and consisted of about 52% calcium sulfate, 33% calcium oxide and 15% coal ash residues. Eleven years after ash application, soil pH is significantly higher in the top 66 cm of the treated sites compared to unamended sites. This has been accompanied by increases in extractable and total calcium and total boron and sulfur with a concomitant reduction in extractable magnesium. Remaining pieces of the applied spent bed material are composed primarily of calcite and quartz with some gypsum associated with large pieces.
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  • 51
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    Plant and soil 155-156 (1993), S. 411-414 
    ISSN: 1573-5036
    Keywords: calcium ; magnesium ; Populus trichocarpa ; potassium ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two clones of Populus trichocarpa varying in growth rate were grown in nutrient solutions with a range of K supply. In the leaves an antagonism between the levels of K and Ca+Mg was found, this was most extreme in the older leaves. Analysis of the contents of these elements in leaf vacuoles and cytoplasm suggests that in the vacuoles the total contents of K+Ca+Mg are controlled to maintain ionic and osmotic balance. At low K supply, the K deficit in the cytoplasm is not balance by accumulation of Ca and Mg, and it is suggested that other organic osmotica may accumulate. The regulation of K+Mg+Ca supplied to the leaves seems to be regulated by the roots and is not simple competition of ions for uptake sites at the plasmalemma.
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  • 52
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    Hydrobiologia 251 (1993), S. 65-72 
    ISSN: 1573-5117
    Keywords: Frozen littoral ; frost heave ; nutrient release ; nitrogen ; phosphorus ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In winter, the littoral substrate of ecotonal zones along north Swedish rivers and lakes freezes. Even if the littoral is flooded at freeze-up, the ice freezes solid to the shore substrate due to low temperature combined with a gradual decrease in water level during winter. Frost conditions were studied during an 8-year period along eight littoral transects in the River Vindelälven and adjacent riverside lagoons. Heavy frost heave with formation of needle ice was observed in several places. Nutrient release was suggested to take place due to the frost process. Sediment was taken to a laboratory experiment where samples with sediment and water were kept under unfrozen and frozen conditions during six months. N (total-N) and Ca were significantly released to the water, while P (total-P) did not show any clear movement from the frozen sediment. The results indicate that frost processes in ecotonal zones are involved in the movement of nutrients between sediment and water.
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  • 53
    ISSN: 1573-5117
    Keywords: calcium ; lake management ; phosphorus ; sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Onondaga Lake is a hypereutrophic, industrially polluted lake located in Syracuse, NY. High hypolimnetic concentrations of H2S that develop after anoxia restrict the accumulation of total Fe2+ due to the formation of FeS, and may limit Fe-PO4 interactions. High water column concentrations of Ca2+ and high rates of CaCO3 deposition occur due to inputs of Ca2+ from an adjacent soda ash manufacturing facility. Patterns of P concentration and other water chemistry parameters in the lower waters, and results from chemical equilibrium calculations, suggest that Ca-PO4 minerals may regulate the supply of P from sediments to the water column in Onondaga Lake. These findings have important management implications for Onondaga Lake. First, declines in water column Ca2+ concentrations due to reductions in industrial CaCl2 input may result in conditions of undersaturation with respect to Ca-PO4 mineral solubility and increases in the release of P from sediments to the water column. Second, introduction of O2 from hypolimnetic oxygenation, as a lake remediation initiative, may enhance P supply from sediments, because of increased solubility of Ca-PO4 minerals at lower pH.
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  • 54
    ISSN: 1573-5117
    Keywords: phosphate ; sediment ; calcium ; iron ; coastal lagoons ; seasonal variations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This article concerns seasonal variations in the phosphate concentrations in two coastal lagoons near Montpellier (Mediterranean coast, France). The o-P concentration in the overlying water is highest during summer. The role of the sediment, particularly that of the different P fractions in the sediment, is discussed. Significant variations, especially in the FeOOH ≈ P fraction, occur. For both Tot-Psed and the Fe00H≈P fraction a gradient from surface to bottom is observed, as well as a distinct decrease in the FeOOH≈P fraction in the surface sediments during summer and autumn. Variations in the FeOOH≈P fraction appear to be compensated by variations in the CaC03≈P fraction. These variations appear to be determined by the ferric hydroxide concentration. This compound represents only a small part (maximally 15%) of the total iron in the sediments and is related to the dissolved oxygen content of the immediately overlying water. Besides the fractions o-P, Fe(OOH)≈P, a large part of the CaC03≈P fraction is potentially bioavailable. A large proportion of the Tot-Psed is therefore bioavailable.[/p]
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  • 55
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    Hydrobiologia 252 (1993), S. 245-256 
    ISSN: 1573-5117
    Keywords: Algae ; ATPase ; calcium ; cadmium ; plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To further study the toxicity of cadmium in the euryhaline alga, Dunaliella bioculata, ATPase activity and Cd2+ interactions were investigated in this species. Ultracytochemical studies showed the presence of ATPase reaction after incubation with Ca2+ and Mg2+, on different cell structures, the cytoplasm, the nucleoplasm, the axoneme and the membrane of the flagellae. In the cytoplasm, the localization of the lead precipates suggests that they are associated with the endoplasmic reticulum. The in vitro measurement of enzyme activity in crude cell extracts obtained by a partial solubilization of deflagellated algae with Triton X100, revealed a high Mg2+ dependent pyrophosphatase activity, a weak Mg2+-ATPase and a Ca2+-ATPase (Km = 0.12 mM) which was little sensitive to vanadate. In these extracts, a Ca2+ dependent ATPase was detected at the level of a double band by a non-denaturing electrophoresis. The same activity was found in the supernatant of sonicated cells in the absence of detergent, which suggests that this ATPase could be a cytosolic enzyme. In plasma membrane fractions, vanadate-sensitive ATPase activity was measured. This reaction was activated either by Mg2+ at relatively low concentrations (Km = 150µm) or by Ca2 +, but required unusually high concentrations of this ion, 50–100 mM. The inhibitory effects of Cd2+ on Ca2+ ATPase activity in cell extracts were compared with those of other cations. The range of toxicity was: Zn2+ 〉 Cd2+ 〉 Cu2+ 〉 La3+ 〉 Co2+. For Cd2+, the IC50 was 42 µM. The nature of inhibition, though, mixed was for the most part competitive, since the competitive constant value (Ki = 7 µM) was lower than the non-competitive constant value (K′i = 35 µM). In plasma membrane fractions, ATPase activity showed a high sensitivity to the heavy metal. It was non-competitively inhibited by cadmium in a narrow range of micromolar concentrations.
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  • 56
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    Fish physiology and biochemistry 11 (1993), S. 25-33 
    ISSN: 1573-5168
    Keywords: signal transduction ; goldfish gonadotropin ; goldfish growth hormone ; GnRH ; dopamine D1 and D2 activation ; calcium ; PKC ; cAMP ; Arachidonic acid ; G-proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Cette revue présente les données expérimentales démontrant l'implication de Ca++, de la protéine kinase C et du métabolismes de l'acide arachidonique dans les mécanismes régulant la sécrétion des hormones gonadotrope (GTH) et de croissance (GH). Des modèles de signaux de transduction de l'action de la gonadolibérine (GnRH) et de la dopamine sur la sécrétion de GTH et de GH sont proposés. Les deux GnRHs existant chez le poisson rouge pourraient se lier au même type de récepteur et activer différentes voies de transduction dans deux différents types cellulaires (GTH vs. GH) ou dans un seul type (GTH).
    Notes: Abstract Evidence for the involvement of Ca2+, protein kinase C, cAMP, and arachidonic acid metabolism in mediating gonadotropin (GTH) and growth hormone (GH) release in the goldfish is reviewed. Models for the signal transduction pathways mediating GTH-releasing hormone (GnRH) and dopamine actions on GTH and GH secretion are postulated. A novel hypothesis that two GnRHs which bind to the same receptor type activate different transduction cascade in two different cell types (GTH vs. GH) as well as within the same cell type (GTH) is presented.
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  • 57
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    Cell biology and toxicology 9 (1993), S. 141-148 
    ISSN: 1573-6822
    Keywords: genistein ; xenobiotic receptor ; calcium ; inositol ; zinc ; nickel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cd2+ provokes inositol trisphosphateproduction and releases stored Ca2+, apparently by binding to a zinc site in the external domain of an orphan receptor. One pM Cd2+ evokes an immediate spike in cytosolic free Ca2+, which is similar to that evoked by bradykinin. Platelet-derived growth factor (PDGF) also increases free Ca2+ in human dermalfibroblasts, but there is a distinct lag before free Ca2+ rises in response to PDGF. Genistein, which selectively inhibits tyrosine kinases, markedly inhibited Ca2+ mobilization evoked by PDGF. Calcium mobilization triggered by cadmium or bradykinin was relatively insensitive to genistein. The PDGF receptor is known to be a tyrosine kinase, whichphosphorylates and thereby activatesphospholipase Cψ, whereas a G protein couples the bradykinin receptor to anotherphospholipase C isoform. These findings support the hypothesis that the orphan receptor triggered by cadmium is coupled to phospholipase C via a G protein.
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  • 58
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    Journal of bioenergetics and biomembranes 25 (1993), S. 377-384 
    ISSN: 1573-6881
    Keywords: (Plant) mitochondria ; NAD(P)H dehydrogenase ; NAD(P)H-ubiquinone oxidoreductase ; rotenone ; Complex I ; calcium ; electrostatic interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Plant (and fungal) mitochondria contain multiple NAD(P)H dehydrogenases in the inner membrane all of which are connected to the respiratory chain via ubiquinone. On the outer surface, facing the intermembrane space and the cytoplasm, NADH and NADPH are oxidized by what is probably a single low-molecular-weight, nonproton-pumping, unspecific rotenone-insensitive NAD(P)H dehydrogenase. Exogenous NADH oxidation is completely dependent on the presence of free Ca2+ with aK 0.5 of about 1 µM. On the inner surface facing the matrix there are two dehydrogenases: (1) the proton-pumping rotenone-sensitive multisubunit Complex I with properties similar to those of Complex I in mammalian and fungal mitochondria. (2) a rotenone-insensitive NAD(P)H dehydrogenase with equal activity with NADH and NADPH and no proton-pumping activity. The NADPH-oxidizing activity of this enzyme is completely dependent on Ca2+ with aK 0.5 of 3 µM. The enzyme consists of a single subunit of 26 kDa and has a native size of 76 kDa, which means that it may form a trimer.
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    Journal of bioenergetics and biomembranes 25 (1993), S. 473-481 
    ISSN: 1573-6881
    Keywords: Mitochondria ; ATP-Mg/Pi carrier ; adenine nucleotide ; calcium ; liver ; kidney ; transport ; phosphate ; magnesium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The mitochondrial ATP-Mg/Pi carrier functions to modulate the matrix adenine nucleotide pool size (ATP + ADP + AMP). Micromolar Ca2+ is required to activate the carrier. Net adenine nucleotide transport occurs as an electroneutral divalent exchange of ATP-Mg2− for HPO 4 2− . A steady-state adenine nucleotide pool size is attained when the HPO 4 2− and ATP-Mg2− matrix/cytoplasm concentration ratios are the same. This means that ATP-Mg2− can be accumulated against a concentration gradient in proportion to the [HPO 4 2− ] gradient that is normally maintained by the Pi/OH− carrier. In liver, changes in matrix adenine nucleotide concentrations that are brought about by the ATP-Mg/Pi carrier can affect the activity of adenine nucleotide-dependent enzymes that are in the mitochondrial compartment. These enzymes in turn contribute to the overall regulation of bioenergetic function, flux through the gluconeogenesis and urea synthesis pathways, and organelle biogenesis. The ATP-Mg/Pi carrier is distinct from other mitochondrial transport systems with respect to kinetics and to substrate and inhibitor sensitivity. It is the only carrier regulated by Ca2+. This carrier is present in kidney and liver mitochondria, but not in heart.
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  • 60
    ISSN: 1573-5044
    Keywords: calcium ; Eustoma grandiflorum ; osmolality ; pH ; plant regeneration ; protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A reliable method has been developed for regeneration of whole plants from isolated protoplasts of five cultivars of lisianthus,Eustoma grandiflorum (Griseb.) Schinners (Gentianaceae). Protoplasts were isolated from either cotyledons or leaves and cultured in agarose beads surrounded by liquid V-KM media containing 5.37 µM 1-naphthyleneacetic acid (NAA) and 2.28 µM zeatin. When microcalli were approximately 1 mm in diameter, the agarose beads were transferred to shoot regeneration media containing 0.1 µM indolebutyric acid (IBA) and 4.44 µM 6-benzylaminopurine (BAP). Shoots were produced from the calli during several sub-culture periods. Protoplast viability and the subsequent regeneration of plants were dependent on calcium levels and growth regulator presence in thein vitro seed germination media, on the osmolality of the protoplast purification solution, and osmolality increase and pH of the culture media. Shoots were rooted in Murashige & Skoog (1962) media containing 5.71 µM indole-3-acetic acid (IAA). Plantlets derived from protoplasts of five lisianthus cultivars (Fresh White, Hakusen, Miss Lilac, Fresh Purple and Doremi Wine Red) have been successfully transferred to the glasshouse.
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    Nutrient cycling in agroecosystems 36 (1993), S. 79-90 
    ISSN: 1573-0867
    Keywords: agriculture ; Sahel ; Sudan ; Mali ; cotton ; fertilization ; nutrient ; soil ; soil degradation ; depletion ; nutrient ; nutrient balance ; nitrogen ; phosphorus ; potassium ; calcium ; magnesium ; acidification ; sustainability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The degree of soil mining by agricultural production in Southern Mali is assessed by calculating nutrient balances: differences between the amount of plant nutrients exported from the cultivated fields, and those added to the fields. Export processes include extraction by crops, losses due to leaching, to erosion, and to volatilization and denitrification. Inputs include applications of fertilizer and manure, restitution of crop residues, nitrogen fixation, atmospheric deposition of nutrients in rain and dust, and enrichment by weathering of soil minerals. Nutrient balances are calculated for N, P, K, Ca, and Mg. Both pessimistic and optimistic estimates are given. The resulting figures indicate, even when the most optimistic estimates are used, large deficits for nitrogen, potassium and magnesium. For the region as a whole, the calculated deficits are -25 kg N/ha,-20 kg K/ha, and -5 kg Mg/ha. Further, acidification is to be expected, in particular in areas where cotton is grown. The deficits are caused by traditional cereal crops, but also by cotton and especially by groundnut. The latter two crops are fertilized, but insufficiently. It is important to note, that the negative figures are not automatic recommendations for application of a specific amount of additional fertilizer. For phosphorus and calcium the balance of the region as a whole appears to be about in equilibrium, but locally large variations may occur. Erosion and denitrification are important causes of nutrient loss, accounting respectively for 17 and 22% of total nitrogen exports. Atmospheric deposition and weathering of minerals in the soil are still important nutrient inputs that contribute as much as nutrients as organic and mineral fertilizer combined. Nutrient depletion is very large in comparison to the amount of fertilizer applied. Drastic options, such as doubling the application of fertilizer or manure, or halving erosion losses, even if feasible, would still not be enough to make up for the calculated deficits. The annual value of withdrawn nutrients, if related to prices of fertilizers, varies between 10,000 and 15,000 FCFA/ha (40-60 US $/ha). Since the estimated average gross margin from farming in this area is 34,000 FCFA/ha (123 US $/ha), soil mining appears to provide an amount equal to 40% of farmers' total income from agricultural activities.
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  • 62
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    Methods in cell science 15 (1993), S. 147-154 
    ISSN: 1573-0603
    Keywords: trypsin ; collagenase ; cardiomyocyte isolation ; calcium ; ethylenediamine tetraacetic acid (EDTA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Maximizing the yield of viable cardiomyocytes suitable for primary cultures is important in the processing of small human myocardial biopsies. The purpose of this study was to establish the conditions of collagenase and trypsin digestion of ventricular myocardium to obtain optimal yields of viable cardiomyocytes. Our results showed that calcium in the digestion solution was toxic to the cells. EDTA chelated calcium in the digestion solution and inhibited collagenase activity. Trypsin increased collagenase activity, especially in the presence of EDTA. The combination of trypsin and collagenase with or without EDTA was more effective than either enzyme alone in increasing cardiomyocyte yield and viability and in minimizing cardiomyocyte damage. By repeating the digestion of a 5 to 20-mg myocardial biopsy, sufficient numbers of viable cardiomyocytes can be obtained for primary culture.
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  • 63
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    Plant and soil 154 (1993), S. 127-132 
    ISSN: 1573-5036
    Keywords: blossom-end rot ; calcium ; greenhouse environment ; humidity ; root-temperature ; salinity ; tomato ; transpiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies of Ca uptake and distribution in relation to environmental variables were used to relate Ca status of tomato fruit to blossom-end rot (BER) incidence. Ca uptake was highly correlated with solar radiation and root temperature. The rate of Ca uptake decreased linearly with increasing salinity. High humidity reduced Ca import by the leaves but increased that by the fruit. While total plant dry weight was reduced more than fruit dry weight by salinity, total Ca uptake and the Ca content of the fruit were decreased similarly. Thus, the concentration of calcium in the fruit was substantially reduced by salinity. The distal half of the fruit contained less Ca than the proximal half. The lowest % Ca was found in the distal placenta and locular tissues, where BER first develops. The incidence of BER was often stimulated more by high salinity achieved with the addition of major nutrients than with NaCl. The cause of BER is usually an interaction between the effects of irradiance and ambient temperature on fruit growth and the effects of environmental stress on calcium uptake and distribution within the whole plant.
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    Biogeochemistry 23 (1993), S. 169-196 
    ISSN: 1573-515X
    Keywords: aluminum ; calcium ; leaching ; magnesium ; potassium ; soil ; sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The effects of three S deposition scenarios — 50% reduction, no change, and 100% increase — on the cycles of N, P, S, K, Ca, and Mg in a mixed deciduous forest at Coweeta, North Carolina, were simulated using the Nutrient Cycling model (NuCM). The purpose of this exercise was to compare NuCM's output to observed soil and streamwater chemical changes and to explore NuCM's response to varying S deposition scenarios. Ecosystem S content and SO4 2− leaching were controlled almost entirely by soil SO4 2− adsorption in the simulations, which was in turn governed by the nature of the Langmuir isotherm set in the model. Both the simulations and the 20-year trends in streamwater SO4 2− concentration suggest that the ecosystem is slowly becoming S saturated. The streamwater data suggest S saturation is occurring at a slower rate than indicated by the simulations, perhaps because of underestimation of organic S retention in the model. Both the simulations and field data indicated substantial declines in exchangeable bases in A and BA soil horizons, primarily due to vegetation uptake. The correspondence of model output with field data in this case was a result of after-the-fact calibration (i.e. setting weathering rates to very low values) rather than prediction, however. Model output suggests that soil exchangeable cation pools change rapidly, undergoing annual cycles and multi-decade fluctuations. Varying S deposition had very little effect upon simulated vegetation growth, nutrient uptake, or N cycling. Varying S deposition strongly affected simulated Ca2+. Mg2+, K+, and P leaching but caused little change in soil exchangeable pools of Ca2+ K+, or P because soil exchangeable pools were large relative to fluxes. Soil exchangeable Mg2+ pools were reduced by high rates of S deposition but remained well above levels sufficient for tree growth. Although the total soil pools of exchangeable Ca2+ and K+ were only slightly affected by S deposition, there was a redistribution of Ca2+ and K+ from upper to lower horizons with increasing S deposition, causing increased base saturation in the deepest (BC) horizon. The 100% increased S deposition scenario caused increasing peaks in simulated Al3+ concentrations in A horizons after 25 years as a result of large seasonal pulses of SO4 2− and lowered base saturation. Simulated soil solution Al3+ concentrations remained well below toxicity thresholds for selected tree species at the site.
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    Fish physiology and biochemistry 10 (1993), S. 491-496 
    ISSN: 1573-5168
    Keywords: calcium ; eel ; stanniocalcin ; metabolic clearance rate ; distribution space ; freshwater ; seawater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The kinetics and hypocalcemic potency of stanniocalcin (STC) were examined in freshwater and seawater eels. The secretion rate and the metabolic clearance rate of STC were calculated from the STC disappearance curve after intra-arterial injection of trout STC. Basal plasma STC concentrations in freshwater and seawater eels did not differ but the STC secretion rate and metabolic clearance rate in seawater eel were 70–75% higher than in FW eel. The increased STC distribution space in seawater eels suggests that the STC receptor density was increased. STC had a higher hypocalcemic potency in seawater than in freshwater eels. These observations support the hypothesis that seawater fish require more hormonal control over transcellular influx of calcium than freshwater fish.
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  • 66
    ISSN: 1573-9104
    Keywords: Lectins ; Concanavalin A ; phytohemagglutinin ; calcium ; sucrose ; intestine ; uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Food lectins are known to affect the absorption of nutrients in experimental animals and altered morphology of the intestinal mucosa due to prolonged feeding of lectins is suggested to be the cause. However, toxic symptoms appear in humans immediately after consumption of lectin rich foods and the mechanism of this immediate action is not known. This study reports the immediate effect of Concanavalin A (Con A) and Phaseolus vulgaris lectin (PHA-P) on the absorption of Ca2+ and sucrose in rat intestine and the possible role of lectin-membrane interactions in the process. Calcium absorption was lowered by Con A, and this was observed to be due to Con A interacting with the intestinal mucosal membrane. Similarly, sucrose absorption was also altered, probably due to Con A binding the intestinal mucosal membrane. On the other hand PHA-P did not have any effect on either sucrose or calcium absorption. Results indicate that the effect on intestinal nutrient absorption depends on the type of lectin used (and its concentration) as well as the type of nutrient, besides the duration of exposure of intestinal mucosa to the lectin.
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    World journal of microbiology and biotechnology 9 (1993), S. 196-201 
    ISSN: 1573-0972
    Keywords: Anabaena doliolum ; calcium ; Chlorella vulgaris ; heavy metals ; immobilization ; magnesium ; organic acids ; pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The potential of alginate-immobilized Anabaena doliolum and Chlorella vulgaris was assessed for removal of nutrients (NO inf3 sup- and NH inf4 sup+ ) and metals (Cr2O inf7 sup2- and Ni2+) at different biomass concentrations (0.05, 0.1, 0.25, 0.49 and 1.22 g dry wt l-1) and pH values (4 to 10). Though uptake of all these substances was higher in concentrated algal beads (0.25, 0.49 and 1.22 g dry wt l-1), their rate of uptake was significantly (P〈0.001) lower than that of low (0.05 g dry wt l-1) cell density beads. For A. doliolum, there was no significant difference in uptake rates for beads having densities of 0.05 and 0.1 g dry wt l-1. Chlorella vulgaris, however, showed maximum efficiency at 0.1 g dry wt l-1. Uptake of both the nutrients and the metals was maximal at pH 7 followed by pH 8, 6, 9, 10, 5 and 4. Of the different substances (organic acids and divalent cations) used, humic acid was most efficient in decreasing metal uptake. Mg2+ was, however, more efficient than Ca2+ in decreasing Ni2+ uptake. Immobilized algae with a cell density of 0.1 g dry wt l-1 were the most efficient for nutrient and metal removal at pH 6 to 8.
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  • 68
    ISSN: 1573-8221
    Keywords: arachidonic acid ; hydrocortisone ; calcium ; JW cells
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    Bulletin of experimental biology and medicine 116 (1993), S. 1417-1420 
    ISSN: 1573-8221
    Keywords: human platelets ; Yersinia toxin ; calcium ; hormones ; aggregation
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    Bulletin of experimental biology and medicine 116 (1993), S. 1414-1416 
    ISSN: 1573-8221
    Keywords: platelets ; guanylate cyclase ; Yersinia toxin ; calcium
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  • 71
    ISSN: 1573-8221
    Keywords: vascular smooth muscles ; contraction ; endothelin ; calcium ; blockers
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  • 72
    ISSN: 1573-0867
    Keywords: Leaching ; calcium ; magnesium ; potassium ; nitrate ; tropical soils ; 15N ; urea ; shifting cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Calcium hydroxide was applied to monolith lysimeters at Onne in south-east Nigeria. Eight lysimeters were cropped with maize followed by upland rice and four were uncropped. The cropped and two uncropped lysimeters received Mg, K and urea in the first season. Two uncropped lysimeters received no fertilizers. Drainage water was collected during the two growing seasons and analyzed for calcium, magnesium, potassium, sodium, nitrate and chloride. The fertilizer applied in the second season was not leached during the year of application. The cropped lysimeters lost 27 percent of the sum of the exchangeable Ca in the soil profile and the calcium added, and 29 percent of the corresponding sum for Mg. With no crop, the losses increased to 34 and 37 percent, respectively, but with no crop or fertilizer, the losses were similar to those from the cropped lysimeters. The loss of potassium ranged from 6 percent from the unfertilized lysimeters to 10 percent in the cropped lysimeters. The amounts of sodium leached ranged from 29 to 35 kg Na ha−1. The bulk of the calcium and magnesium leached from calcium hydroxide and fertilizers occurred in the second season when the loss was in good agreement with the amount of nitrate lost giving (Ca + Mg)/NO3 charge ratios of approximately one. Urea increased the amount of nitrate leached and led to a corresponding increase in the amounts of calcium and magnesium lost in the drainage water. The charge ratio remained unchanged when the cations were leached only with nitrate derived from the mineralization of soil organic matter. In the cropped lysimeters, this source accounted for about four times more nitrate in the drainage water than the fertilizer.
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    Cellular and molecular life sciences 48 (1992), S. 516-519 
    ISSN: 1420-9071
    Keywords: Lipid ; neuroepithelial cells ; calcium ; neurulation ; neural tube ; chick embryo ; mouse embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In situ precipitation of calcium (Ca2+) with fluoride and antimonate shows that Ca2+-specific precipitate is localized almost exclusively within lipid droplets of neuroepithelial cells during neural tube formation in chick and mouse embryos. The density of Ca2+ precipitate within lipid droplets is generally greater in the apical ends of cells situated in regions of the neuroepithelium that are actively engaged in bending. These findings suggest that lipid droplets, in addition to providing a source of metabolic fuel for developing neuroepithelial cells, also serve as Ca2+-storage and-releasing sites during neurulation.
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    Molecular and cellular biochemistry 114 (1992), S. 97-103 
    ISSN: 1573-4919
    Keywords: magnesium ; calcium ; hydrogen ion ; fluorescent dyes ; heart cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Several aspects of Mg2+ homeostasis were investigated in cultured chicken heart cells using the fluorescent Mg2+ indicator, FURAPTRA. The concentration of cytosolic Mg2+ ([Mg2+]i) is 0.48 ± 0.03 mM (n = 31). To test whether a putative Na/Mg exchange mechanism controls [Mg2+]i below electrochemical equilibrium, we manipulated the Na+ gradient and assessed the effects on [Mg2+]i. When extracellular Na+ was removed, [Mg2+]i increased; this increase was not altered in Mg-free solutions, but was attenuated in Ca-free solutions. A similar increase in [Mg2+]i, which was dependent upon extracellular Ca2+, was observed when intracellular Na+ was raised by inhibiting the Na/K pump with ouabain. These results do not provide evidence for Na/Mg exchange in heart cells, but they suggest that Ca2+ can modulate [Mg2+]i. In addition, removing extracellular Na+ caused a decrease in intracellular pH (pHi), as measured by pH-sensitive microelectrodes, and this acidification was attenuated when Cat+ was also removed from the solution. These results suggest that Ca2+ and H+ interact intracellularly. Since changes in the Na+ gradient can also alter pHi, we questioned whether pH can modulate [Mg2+]i. pHi was manipulated by the NH4Cl prepulse method. NH4 +-evoked changes in pHi, as measured by the fluorescent indicator BCECF, were accompanied by opposite changes in [Mg2+]i; [Mg2+]i changed by −0.16 mM/unit pH. These NH4 +-evoked changes in [Mg2+]i were not caused by movements of Mg2+ or Ca2+ across the sarcolemma or by changes in cytosolic Ca2+. Additionally, pHi was manipulated by changing extracellular pH (pHo). When pHo was decreased from 7.4 to 6.3, pHi decreased by 0.64 units and [Mg2+]i increased by 0.12 mM; in contrast, when pHo was raised from 7.4 to 8.3, pHi increased by 0.6 units and [Mg2+]i did not change significantly. The results of our investigations suggest that Ca 2+ and H+ can modulate [Mg2+]i, probably by affecting cytosolic Mg2+ binding and/or subcellular Mg2+ transport and that such redistribution of intracellular Mg2+ may play an important role in Mg2+ homeostasis in cardiac cells.
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  • 75
    ISSN: 1573-4919
    Keywords: calcium ; regucalcin ; deoxyuridine 5′-triphosphatase ; rat liver cytosol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on deoxyuridine 5′-triphosphatase (dUTPase) in the cytosol of rat liver was investigated. Addition of Ca2+ up to 5.0 µM to the enzyme reaction mixture caused a significant decrease of dUTPase activity, while Zn2+, Cd2+, Co2+, Al3+, Mn2+ and Ni2+ (10 µM) did not have an appreciable effect. The Ca2+-induced decrease of dUTPase activity was reversed by the presence of regucalcin; the effect was complete at 1.0 µM of the protein. Regucalcin had no effect on the basal activity of the enzyme. Meanwhile, the reversible effect of regucalcin on the Ca2+ (10 µM)-induced decrease of dUTPase activity was not altered by the coexistence of Cd2+ or Zn2+ (10 µM). The present data suggest that liver cytosolic dUTPase is uniquely regulated by Ca2+ of various metals, and that the Ca2+ effect is reversed by regucalcin.
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    Molecular and cellular biochemistry 111 (1992), S. 71-76 
    ISSN: 1573-4919
    Keywords: neutrophil ; free oxygen radicals ; calcium ; endothelial cell growth factors ; prostaglandins ; indomethacin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The exposure of cardiac cells to OFR generated artificially, showed a marked decrease (p 〈 0.01) in cellular utilization of glucose alongwith a significant decrease in calcium uptake (p 〈 0.05). We have also provided evidence for a direct relationship of neutrophil OFR production with the extent of myocardial ischemia in patients of myocardial infarction. Our data provides evidence for implication of OFR in myocardial injury and the pivotal role played by modulators like calcium, ECGF and prostaglandins in potentiating damage to the myocardium.
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    Molecular and cellular biochemistry 111 (1992), S. 17-24 
    ISSN: 1573-4919
    Keywords: ischemia/reperfusion injury ; oxygen radicals ; sarcoplasmic reticulum ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The purpose of this study was to explore the role of singlet oxygen in cardiovascular injury. To accomplish this objective, we investigated the effect of singlet oxygen [generated from photoactivation of rose-bengal] on the calcium transport and Ca2+-ATPase activity of cardiac sarcoplasmic reticulum and compared these results with those obtained by superoxide radical, hydrogen peroxide and hydroxyl radical. Isolated cardiac SR exposed to rose bengal (10 nM) irradiated at (560 nm) produced a significant inhibition of Ca 2+ uptake; from 2.27 ± 0.05 to 0.62 ± 0.05 µmol Ca+/mg.min (mean ± SE) (P 〈 0.01) and Ca2+-ATPase activity from 2.08 ± 0.05 µmol Pi/min. mg to 0.28 ± 0.04 µmol Pi/min. mg (mean ± SE) (P 〈 0.01). The inhibition of calcium uptake and Ca2+-ATPase activity by rose bengal derived activatedoxygen (singlet oxygen) was dependent on the duration of exposure and intensity of light. The singlet oxygen scavengers ascorbic acid and histidine significantly protected SR Ca2+-ATPase against rose bengal derived activated oxygen species but superoxide dismutase and catalase did not attenuate the inhibition. SDS-polyacrylamide gel electrophoresis of SR exposed to photoactivated rose bengal up to 14 min, demonstrated complete loss of Ca2+-ATPase monomer band which was significantly protected by histidine. Irradiation of rose bengal also caused an 18% loss of total sulfhydryl groups of SR. On the other hand, superoxide (generated from xanthine oxidase action on xanthine) and hydroxyl radical (0.5 mM H2O2 + Fe2+ -EDTA) as well as H2O2 (12 mM) were without any effect on the 97,000 dalton Ca2+-ATPase band ofsarcoplasmic reticulum. The results suggest that oxidative damage of cardiac sarcoplasmic reticulum may be mediated by singlet oxygen. This may represent an important mechanism by which the oxidative injury to the myocardium induces both a loss of tension development and arrhythmogenesis.
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    The journal of membrane biology 125 (1992), S. 243-253 
    ISSN: 1432-1424
    Keywords: ATP ; FRTL-5 ; thyroid gland ; chloride currents ; inositol trisphosphate ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effect of external ATP on both the membrane potential and the transmembrane current of the thyroid cell line FRTL-5 has been investigated in the patch-clamp whole-cell recording configuration. In the resting situation the membrane potential is around −70 mV and the membrane acts like a K+-sensitive electrode. Application of ATP at concentrations higher than 1 μ M elicited an increase in Cl− conductance, responsible for a membrane depolarization which could be blocked by preincubation with the P2-antagonist quinidine. Chelation of intracellular Ca2+ also blocked the ATP induced changes in membrane potential and Cl− current. Intracellular perfusion with inositol trisphosphate (IP3) (50 μ M) also stimulated a Cl− current which mimicked the response induced by ATP. ATP is able to initiate a response in the absence of extracellular Ca2+, but also opens a Ca2+-influx pathway, as demonstrated by a secondary response upon Ca2+ readmission in the external medium, in the continued presence of ATP. ADP and ATP-γS were able to mimic the ATP response, whereas AMP and adenosine were unable to elicit a Cl− current. The P2X receptor agonist α,β-methyleneATP was without effect as was the P2Y receptor agonist 2-methylthio ATP. We conclude that ATP is able to elicit a large IP3-mediated Ca2+-dependent Cl− current and membrane depolarization via a novel P2-type purinergic receptor.
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  • 79
    ISSN: 1432-1424
    Keywords: membrane potential ; vesicular stomatitis virus ; MDCK ; calcium ; pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary We used fluorescence microscopy of Madin-Darby Canine Kidney (MDCK) cells grown on polycarbonate filters to study a possible link between plasma membrane electrical potential (ΔΨpm) and infectivity of vesicular stomatitis virus (VSV). Complete substitution of K+ for extracellular Na+blocks VSV infection of MDCK cells as well as baby hamster kidney (BHK) cells. When we independently perfused the apical and basal-lateral surfaces of high resistance monolayers, high K+ inhibited VSV infection of MDCK cells only when applied to the basal-lateral side; high K+ applied apically had no effect on VSV infection. This morphological specificity correlates with a large decrease in ΔΨpm of MDCK cells when high K+ buffer is perfused across the basal-lateral surface. Depolarization of the plasma membrane by 130 mm basal K+ causes a sustained increase of cytosol pH in MDCK cells from 7.3 to 7.5 as reported by the fluorescent dye BCECF. Depolarization also causes a transient increase of cytosol Ca2+ from 70 to 300 nm as reported by the dye Fura-2. Neither increase could explain the block of VSV infectivity by plasma membrane depolarization. One alternative hypothesis is that ΔΨpm facilitates membrane translocation of viral macromolecules as previously described for colicins, mitochondrial import proteins, and proteins secreted by Escherichia coli.
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    Plant and soil 139 (1992), S. 285-294 
    ISSN: 1573-5036
    Keywords: Brassica ; B. campestris ; B. carinata ; B. juncea ; B. naptus ; B. nigra ; B. oleracea ; calcium ; chloride ; potassium ; magnesium ; nitrogen ; sodium ; nutrients ; salinity ; salt-tolerance ; seawater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The growth of six rapid-cycling lines of Brassica species, B. napus, B. campestris, B. nigra, B. juncea, B. oleracea and B. carinata was inhibited by seawater salinity. Based on the change in dry matter reduction relative to the control at varying concentrations of salts (4, 8 and 12 dS m-1), the relative salt tolerance of these species was evaluated. B. napus and B. carinata were the most tolerant and most sensitive species, respectively, while the other four species were moderately tolerant. The influence of seawater on the concentrations of 12 elements including macronutrients and micronutrients in the shoots of these Brassica plants was characterized to determine the relationship between nutritional disturbance and relative salt tolerance. It was found that seawater salinity had a significant effect on the concentrations of Ca, Mg, K, Cl, Na and total N in the shoots of these plants but only the change in Ca concentration was significantly related to the relative salt tolerance of these six rapid-cycling Brassica species according to a rank analysis of the data. This finding indicates that Ca may play a regulatory role in the responses of Brassica species to saline conditions.
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    Plant and soil 147 (1992), S. 293-303 
    ISSN: 1573-5036
    Keywords: amino acid uptake ; aluminum ; calcium ; germination ; pH ; Phaseolus vulgaris ; plant establishment ; Rhizoctonia solani ; seedling exudation ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil born fungi such as Phytium ultimum, Fusarium ssp., and Rhizoctonia solani (Kühn) severely restrict stand establishment of common bean (Phaseolus vulgaris L.) on acid soils of the Tropics. Calcium application is known to alleviate fungal infection in many legumes but the causes are still unclear. To investigate environmental factors and physiological mechanisms involved, growth chamber experiments were conducted with an acid sandy soil from Mexico. Treatments were soil liming at a rate of 0.67 g Ca(OH)2 kg-1, gypsum application at 0.49 g CaSO4 2H2O kg-1 soil placed around the seed, and an untreated control. Beans were grown under three temperature regimes with constant night and one constant day vs. two sinusoidal day temperatures. To examine patterns of seed and seedling exudation at regular intervals leachates of germinating seeds were collected on filter paper soaked with equilibrium solutions from soils of the three treatments. The severity of root rot in the control treatment was highest when plants were stressed by temperature extremes. At a sinusoidal day temperature peaking at 40°C soil liming and gypsum application to the seed increased the number of healthy seedlings similarly by over 60%. However, only liming which effectively eliminated growth constraints by low pH and high aluminum concentrations led to an increase in hypocotyl elongation by 22% and in total root length by 8%. Both calcium amendments increased the calcium and potassium contents in the hypocotyl tissue. From seeds exposed to the equilibrium solution of unlimed soil with pH 3.7, 1 mM Ca, and 0.6 mM Al considerable amounts of amino acids and carbohydrates were leached. In contrast, exposure to the equilibrium solution from limed soil with pH 4.3, 3 mM Ca, and negligible concentrations of Al led to a net uptake of amino acids and decreased leaching of carbohydrates. Exposure to the equilibrium solution of the gypsum treatment with pH 3.6, 20 mM Ca, and 1.2 mM Al resulted in a somewhat smaller net uptake of amino acids compared to liming. During germination pH around the seeds steeply increased in the untreated control but significantly less with both amendments. The results indicate that pH and the Ca/Al ratio in the soil solution around bean seeds determine their pattern of exudation and solute uptake. For bean germination and early growth on acid soils locally placed application of small amounts of gypsum as seed pelleting seems as effective as soil liming in reducing the incidence of root rot. The results indicate that this may be accomplished by decreasing the amount of leachates available for fungal development.
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  • 82
    ISSN: 1573-5036
    Keywords: aluminium ; aluminium tolerance ; calcium ; magnesium ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The activities of inorganic, monomeric aluminium (Al) species in the root environment are important in the toxicity of Al to plant roots, which may be ameliorated by increased activities of basic cations. Additionally, it has been suggested that electro-chemical processes in walls of root cells play a role in Al tolerance. Empirical models were proposed to accomodate genetic and calcium (Ca) and magnesium (Mg) ameliorative effects on Al toxicity. The models were tested using data from a solution culture study (with ionic strength 1.6 to 8.6 mM) in which wheat (Triticum aestivum L.) cvv. Warigal (Al-sensitive) and Waalt (Al-tolerant) were grown for 28 d at 0, 10 and 20 μM Al, in factorial combination with 200, 400, 800 and 1600 μM Ca and 100, 200, 400 and 800 μM Mg. There was a poor relationship between relative total dry mass (TDM) (calculated as a percentage of the average TDM of each cultivar in the absence of added Al) and the activity of Al3+ or the sum of the activities of the monomeric Al species in solution. A model based on the ratios of activities of cations in solution, taking valency into consideration, was more successful, accounting for ca 85% of the observed variation in relative TDM. There were no systematic variations between observed values and those estimated by the model.
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  • 83
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    Plant and soil 143 (1992), S. 19-31 
    ISSN: 1573-5036
    Keywords: ammonium ; calcium ; carboxylate/organic nitrogen ratio ; 15N isotope ; nitrate ; nitrogen cycling ; perennial ryegrass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Solution culture studies have shown that plant uptake of NH4 + and NO3 - can be improved by increasing the concentration of Ca2+ in the root environment: the same may be true for grass grown in soil culture. An experiment was set up to see whether gypsum (CaSO4 2H2O) increased the rate at which perennial ryegrass absorbed 15NH4 + and 15NO3 - from soil. The results demonstrated that gypsum increases the rates of uptake of both NH4 + and NO3 - by perennial ryegrass. However because there was little potential for mineral-N loss from the experimental system, either by gaseous emission or by N immobilization, long term improvements in fertilizer efficiency were not observed. Nitrogen cycling from shoots to roots commenced once net uptake of N into plants had ceased. Labelled N transferred thus to roots underwent isotopic exchange with unlabelled soil N. It was suggested that this exchange of N might constitute an energy drain from the plant, if plant organic N was exchanged for soil inorganic N. The fact that the exchange occurred at all cast doubt on the suitability of the 15N-isotope dilution technique for assessing fertilizer efficiency in medium to long term experiments. There was evidence that the ‘extra’ NO3 --N taken up by plants on the all-nitrate treatments as a result of gypsum application, was reduced in root tissue rather than in shoots, but to the detriment of subsequent root growth and N uptake.
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  • 84
    ISSN: 1573-5036
    Keywords: calcium ; exchangeable cations ; magnesium ; organic phosphorus ; phosphate fractionation ; potassium ; superphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The annual herbage dry matter yield, herbage P concentrations and quantities of P, K, Mg and Ca cycled by grazing sheep were calculated for a 37 year-old grazed pasture supplied with superphosphate at rates of 0, 188 and 376 kg ha-1 annually. The amount and distribution of inorganic and organic P and exchangeable K, Mg and Ca in the soil below the three grazed treatments was also measured and compared with that below a wilderness area which had not been used for agriculture. Increasing rates of superphosphate increased herbage dry matter yield, herbage P concentrations and thus the amounts of P ingested and, in turn, excreted by the grazing sheep. Annual quantities of K, Mg and Ca cycled back to the pasture in the form of excreta were also increased. The increased cycling of K by animals induced by increasing superphosphate applications resulted in greater losses of K and consequently concentrations of exchangeable and non-exchangeable ‘fixed’ K in soil decreased in the order wilderness 〉 control 〉 188 〉 376. Differences were evident to a depth of 20 cm. Some losses of Mg during cycling also occurred and concentrations of exchangeable Mg followed the order: wilderness 〉 control 〉188=376. Concentrations of exchangeable Ca increased with pasture development due to additions of Ca in lime and superphosphate. Concentrations of total soil organic P (0–4 cm) increased in the order: wilderness 〈 control 〈188〈376 but for inorganic P and total P the control had a lower content than wilderness indicating losses of P during cycling in the grazed control treatment. Phosphorus fractionation suggested that with increasing superphosphate rates inorganic P primarily accumulated in a form adsorbed to Al hydrous oxides and as calcium phosphate compounds whilst organic P accumulated in both labile forms and forms associated with humic compounds. In the fertilised sites both inorganic and organic P accumulated in the soil profile to a depth of 20 cm.
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  • 85
    ISSN: 1573-5036
    Keywords: aluminium toxicity ; Arachis hypogaea L. ; Bradyrhizobium ; calcium ; magnesium ; solution culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract While considerable information has been presented recently on the alleviating effects of calcium (Ca) on aluminium (Al) toxicity, the interaction between Ca and Al on nodulation and N2-fixation of legumes is little understood. A 28 d solution culture experiment using groundnut (Arachis hypogaea L.) cv. Matjam was conducted to evaluate the effects of four Ca concentrations and four Al levels on nodule development, N2-fixation and plant growth. The Ca concentrations were maintained at 500, 1000, 2500 or 5000 μM, and the sum of activities of monomeric Al species (ΣaAlmono) were 0, 15, 30 and 60 μM. With ΣaAlmono≥30 μM in solution, the time to appearance of the first nodule increased, and, with 60 μM ΣaAlmono in solution, plants remained chlorotic throughout the experiment. Activities≥30 μM reduced nodule number and nodule dry mass per plant, particularly with high (5000 μM) Ca in solution. Also, plant top growth was decreased at ΣaAlmono≥30 μM; the effect only being alleviated by 1000 μM Ca at 30 μM ΣaAlmono. The Ca concentration in the youngest expanded leaf (YEL) increased with increased Ca concentration in solution, but was little affected by Al treatment. Nitrogen concentrations mirrored treatment effects on nodule number and nodule dry mass; Al in solution decreased the N concentration particularly with 5000 μM Ca in solution. Furthermore, increased Ca and Al in solution decreased the Mg concentration in the YEL. This suggested that the absence of any alleviating effect of Ca and Al toxicity (indeed the opposite effect was often observed) resulted from interference in Mg nutrition.
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  • 86
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    Plant and soil 146 (1992), S. 45-54 
    ISSN: 1573-5036
    Keywords: calcium ; cowpea ; efficiency ; genotypic differences ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The cowpea (Vigna unguiculata (L.) Walp.) cultivars TVu 354 and Solojo were grown in solution culture at 10 to 1000 μM Ca supply. The Ca supply did not vary by more than 10% during the experiment. The pH value was kept constant within 0.1 units at 4.0 by automatic titration. The cultivar TVu 354 proved to be much more Ca-efficient than Solojo. At 10 μM Ca supply Solojo died, whereas TVu 354 was hardly affected in dry matter production. The differences in Ca efficiency were independent of the P supply. They could not be explained by differences in Ca uptake or Ca concentrations in the plant tissue. Short-term studies using 45Ca, both in the dark and in the light, indicated better transport of Ca from the roots to the shoots and within the shoots to the younger leaves in the Ca-efficient cultivar TVu 354. However, the main reason for the differences between the cultivars in sensitivity to low Ca supply were differences in the Ca requirement of the plant tissue to maintain tissue organization and function. Sequential fractionation of the freeze-dried leaf tissue with hot water, 0.5 M NaCl, 1 M CH3COOH, and 2 M HCl did not reveal cultivar differences in Ca binding state. The results clearly show that considerable genetic potential in tolerance to low Ca supply exists in cowpea. However, a better understanding of the physiological/biochemical reasons for low internal Ca requirement is needed.
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  • 87
    ISSN: 1573-5044
    Keywords: calcium ; plant cell suspension culture ; tracheary element differentiation ; xylogenesis ; Zinnia elegans L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simplified medium has been developed for the differentiation of tracheary elements in suspension cultures of mesophyll cells of Zinnia elegans L. All inorganic salts contained in media used previously were retained in the simplified medium, but most were reduced in concentration. The only organic supplements required for optimum differentiation were thiamine and nicotinic acid, in addition to the plant growth regulators N6-benzylaminopurine and α-naphthyleneacetic acid, and sucrose as a carbon source. Mannitol, an osmoticum, was necessary for rapid, synchronous differentiation. This simplified medium is particularly suitable for studies of the role of Ca2+ in tracheary element differentiation due to the elimination of myo-inositol, an intermediate in the phosphatidyl inositol signal transduction pathway and reduction in the concentrations of Mg2+ and Mn2+, which block calcium channels. It is also possible to eliminate EDTA from the medium, enabling studies using specific calcium chelators. Additional culture variables for the optimization of differentiation are discussed.
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  • 88
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    Hydrobiologia 228 (1992), S. 43-54 
    ISSN: 1573-5117
    Keywords: phosphate adsorption ; aluminium ; iron ; calcium ; sediments ; Rio de la Plata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study of the phosphate adsorption onto the bottom sediments of the Rio de la Plata has been made with the aim to understand the dynamics of this compound in the fluvio-marine system. Previous to the chemical analysis of the phosphate content, an extraction of the different adsorbed phosphate fractions have been made. In addition to phosphate, calcium, iron and aluminium in the sediment samples were determined. The phosphate is associated to the fine fractions of the sediments and good correlations with Al and Fe content in the bottom sediments were found. There is a relative decrease of the adsorbed phosphate on the bottom sediment in the areas where the influence of the marine water is more conspicuous; it is explained by the pH increase of the estuary waters due to the mixture with the marine waters. A hypothesis about the role of the ionic strength and the pH on the phosphate adsorption process is suggested.
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  • 89
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    Plant growth regulation 11 (1992), S. 277-291 
    ISSN: 1573-5087
    Keywords: Cicer arietinum L. ; calcium ; cytokinins ; phytochrome ; reserve mobilization ; seed germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Eight cytokinins detected in germinated chick-pea (Cicer arietinum L. var. Castellana) seeds were first present in the embryonic axes but appeared in the cotyledons after 12h of germination. The cytokinins detected in the cotyledons originate in the embryonic axes, but no passage of these substances from the cotyledons to the axes was detected, except when the seeds were treated with red light. It is concluded that the role played by the embryonic axis in mobilizating the main reserves of the cotyledons is mainly effected through these cytokinins. Both natural and synthetic cytokinins exert an important regulatory role in the hydrolysis of reserve proteins and calcium could be involved as an intermediate.
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  • 90
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    Journal of bioenergetics and biomembranes 24 (1992), S. 219-226 
    ISSN: 1573-6881
    Keywords: Phototransduction ; vision ; rods and cones ; G-proteins ; transducin ; signal transduction ; recoverin ; kinases ; channels ; calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract A flash of light initiates a cascade of biochemical reactions inside vertebrate photoreceptor cells, culminating in hydrolysis of intracellular cyclic GMP and hyperpolarization of the cell. The cell recovers by shutting down this cascade and resynthesizing cGMP. Many of the reactions responsible for the excitation and recovery phases of the photoresponse have been identified. Here I review some characteristics of the proteins that participate in these reactions.
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  • 91
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    Journal of bioenergetics and biomembranes 24 (1992), S. 111-117 
    ISSN: 1573-6881
    Keywords: Mitochondrial channels ; selectivity ; patch-clamp ; permeability transition ; calcium ; magnesium ; Cyclosporin A (rat liver mitochondria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Single-channel electrophysiological recordings from rat liver mitoplast membranes showed that the 1.3-nS mitochondrial megachannel was activated by Ca++ and inhibited by Mg++, Cyclosporin A, and ADP, probably acting at matrix-side sites. These agents are known to modulate the so-called mitochondrial permeability transition pore (Gunter, T. E., and Pfeiffer, D. R. (1990)Am. J. Physiol. 258, C755–C786) in the same manner. Furthermore, the megachannel is unselective, and the minimum pore size calculated from its conductance is in agreement with independent estimates of the minimum size of the permeabilization pore. The results support the tentative identification of the megachannel with the pore believed to be involved in the permeabilization process.
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  • 92
    ISSN: 1572-9729
    Keywords: calcium ; fine roots ; nitrogen ; northern hardwood ; nutrient dynamics ; seasonality ; soils ; sulfur ; vegetation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seasonal dynamics of S, Ca and N were examined at the Huntington Forest, a northern hardwood ecosystem in the central Adirondacks of New York for a period of 34 months (1985–1988). Solute concentrations and fluxes in bulk precipitation, throughfall (TF) and leachates from the forest floor, E horizon and B horizon were quantified. Both above and below-ground elemental fluxes mediated by vegetation (e.g. uptake, litter inputs, and fine roots production) were also determined. The roles of abiotic and biotic processes were ascertained based on both changes in solute concentrations through the strata of the ecosystem as well as differences between dormant and growing seasons. Concentrations of SO4 2−, NO3 −, NH4 + and Ca2+ were greater in TF than precipitation. Forest floor leachates had greater concentrations of SO4 2−, NO3 − + NH4 + and Ca2+ (9, 6 and 77 µeq L−1, respectively) than TF. There were differences in concentrations of ions in leachates from the forest floor between the dormant and growing seasons presumably due to vegetation uptake and microbial immobilization. Concentrations and fluxes of NO3 − and NH; were greatest in early spring followed by a rapid decline which coincided with a demand for N by vegetation in late spring. Vegetation uptake (44.7 kg N ha−1 yr−1 ) could account for the low leaching rates of N03 −. Within the mineral soil, changes with soil depth and the absence of seasonal patterns suggest that cation exchange (Ca+) or anion sorption (SO4 2−) are primarily responsible for regulating solute concentrations. The increase in SO4 2− concentration after leachates passed through the mineral soil may be attributed to desorption of sulfate that was adsorbed during an earlier period when SO4 2− concentrations would have been greater due to elevated S inputs.
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  • 93
    ISSN: 1573-5087
    Keywords: Abscisic acid ; calcium ; calmodulin ; ethylene forming enzyme ; Helianthus annuus ; methyl jasmonate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The conversion of 1-aminocyclopropane 1-carboxylic acid (ACC) to ethylene by hypocotyl segments of sunflower (Helianthus annuus L.) seedlings was inhibited by abscisic acid (ABA) and methyl jasmonate (Me-Ja), and this inhibitory effect increased with increasing concentration of both growth regulators. On the contrary, CaCl, enhanced ACC conversion to ethylene at the concentrations of 10-4 M and 5 x 10-4 M, however lower and higher concentrations had no significant action. CaCl, (5 x 10-4M) seemed to magnify the inhibition of the reaction induced by ABA, whereas it reduced (5 x 10-4M) and even abolished (10-3M) the inhibitory action of Me-Ja. The results obtained with a Ca2+ chelator (EGTA), a Ca2+ channel blocker (nifedipine) and calmodulin antagonists (W7 and TFP), given in association with ABA or Me-Ja, suggested that calcium was involved in the inhibition of ACC conversion to ethylene by ABA and Me-Ja through an interaction with calmodulin. However, the mechanism of action of the two growth regulators seemed to be different, since all treatments which resulted in a decrease in cytosolic Ca2+ concentration or in calmodulin action induced a decrease in the effect of ABA and an increase in the effect of Me-Ja.
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  • 94
    ISSN: 1573-5087
    Keywords: Calmodulin ; gravitropism ; growth ; calcium ; auxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We examined the effect of calmodulin (CaM) antagonists applied at the root tip on root growth, gravity-induced root curvature, and the movement of calcium across the root tip and auxin (IAA) across the elongation zone of gravistimulated roots. All of the CaM antagonists used in these studies delayed gravity-induced curvature at a concentration (1 μM) that did not affect root growth. Calmodulin antagonists (≧ 1μM) inhibited downward transport of label from 45Ca2+ across the caps of gravistimulated roots relative to the downward transport of 45Ca2+ in gravistimulated roots which were not treated with CaM antagonists. Application of CaM antagonists at the root tip (≧ 1μM) also decreased the relative downward movement of label from 3H-IAA applied to the upper side of the elongation zone of gravistimulated roots. In general, tip application of antagonists inhibited neither the upward transport of 45Ca2+ in the root tip nor the upward movement of label from 3H-IAA in the elongation zone of gravistimulated roots. Thus, roots treated with CaM antagonists (≧ 1 μM) become less graviresponsive and exhibit reduced or even a reversal of downward polarity of calcium transport across the root tip and IAA transport across the elongation zone. The results indicate that calmodulin-regulated events play a role in root gravitropism.
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  • 95
    ISSN: 1573-515X
    Keywords: calcium ; exchangeable cations ; leaching ; magnesium ; mineral weathering ; potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Overstory species influence the distribution and dynamics of nutrients in forest ecosystems. Ecosystem-level estimates of Ca, Mg, and K pools and cycles in 50-year old Douglas-fir and red alder stands were used to determine the effect of overstory composition on net cation removal from the mineral soil, i.e. cation export from the soil in excess of additions. Net cation removal from Douglas-fir soil was 8 kg Ca ha−1 yr−1, 1 kg Mg ha−1 yr−1, and 0.3 kg K ha−1 yr−1. Annual cation export from soil by uptake and accumulation in live woody tissue and O horizon was of similar magnitude to leaching in soil solution. Atmospheric deposition partially off-set export by adding cations equivalent to 28–88% of cation export. Net cation removal from red alder soil was 58 kg Ca ha−1 yr−1, 9 kg Mg ha−1 yr−1, and 11 kg K ha−1 yr−1. Annual cation accumulation in live woody tissue and O horizon was three times greater than in Douglas-fir, while cation leaching in soil solution was five to eight times greater. The lack of excessive depletion of exchangeable cations in the red alder soil suggests that mineral weathering, rather than exchangeable cations, was the source of most of the removed cations. Nitric acid generated during nitrification in red alder soil led to high rates of weathering and NO3-driven cation leaching.
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  • 96
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    Plant foods for human nutrition 42 (1992), S. 231-237 
    ISSN: 1573-9104
    Keywords: Cocoa beverage ; hydrocyanate ; oxalate ; phytate ; calcium ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Hydrocyanate, oxalate, phytate, calcium and zinc were determined in five brands of cocoa beverage which were coded NC, BT, PN, CT and CA. Hydrocyanate ranged from 5.40 to 9.64 mg/100 g dry matter (DM), oxalate 68 to 146 mg/100 g DM, phytate 590 to 750 mg/100 g DM, calcium 28.7 to 116.4 mg/100 g DM and zinc 0.516 to 0.675 mg/100 g DM. The computed phytate:zinc, calcium:phytate and [calcium] [phytate]/[zinc] molar ratios ranged from 89 to 132, 0.80 to 3.01 and 0.64 to 3.03 respectively. The discussion is focused on toxic levels of hydrocyanate and oxalate, and the significance of the molar ratios in predicting the bioavailability of dietary zinc.
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  • 97
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    Bulletin of experimental biology and medicine 113 (1992), S. 178-180 
    ISSN: 1573-8221
    Keywords: La3+ ; calcium ; erythrocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 98
    ISSN: 1573-8221
    Keywords: parathyroid glands ; calcium ; parathyroid hormone ; hypothalamo-hypophyseal system ; vasopressin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 99
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    Cellular and molecular life sciences 47 (1991), S. 224-226 
    ISSN: 1420-9071
    Keywords: aluminium ; snail ; shell-repair ; calcium ; phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In AlCl3-injected shell-repairing snails,Helix pomatia L., the Al-associated decrease of the weights of the shell-repair membranes was unrelated to the Al-concentration in the membranes. In the haemolymph the concentration of Al was related to the dose of injected Al, while the concentration of Ca was increased by the highest Al-dose only. No phosphate was detected in either controls or Al-injected snails. It is concluded that Al inhibits the growth of the CaCO3-crystals by mechanisms other than incorporation in, or adsorption to, the crystals.
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  • 100
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    Cellular and molecular life sciences 47 (1991), S. 1201-1208 
    ISSN: 1420-9071
    Keywords: Pancreatic islet ; β-cell ; calcium ; protein kinase C down-regulation ; sensitization ; desensitization ; insulin secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The role of protein kinase C and Ca2+ in glucose-induced sensitization/desensitization of insulin secretion was studied. A 22–24h exposure of mouse pancreatic islets to glucose (16.7 mmol/l) in TCM 199 culture medium, with 0.26 mmol/l or 1.26 mmol/l Ca2+, reduced total islet protein kinase C activity to approx. 85% and 60% of control values, respectively. At 0.26 mmol/l Ca2+ in TCM 199 medium, exposure to glucose (16.7 mmol/l) led to a potentiation of both phase 1 and phase 2 of glucose-induced insulin secretion, and caused a shift in the dose-response curve with 10 mmol/l and 16.7 mmol/l glucose exhibiting equipotent effects in stimulation of insulin secretion. In glucose-sensitized islets, the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (0.16 μmol/l) did not further potentiate induction of secretion by 10 mmol/l or 16.7 mmol/l glucose. At 3.3 mmol/l glucose, however, phorbol ester-induced secretion was augmented, and was characterized by a faster onset of secretion in glucose-sensitized islets relative to control islets. In contrast, a partial reduction in arachidonic acid (100 μmol/l)-induced insulin release was observed in glucose-sensitized islets in the absence of extracellular Ca2+. Increasing the Ca2+ concentration to 1.26 mmol/l in TCM 199 during the 22–24h exposure to glucose (16.8 mmol/l) led to inhibition of phase 1 and abolition of phase 2 of glucose (10 mmol/l, 16.7 mmol/l)-induced insulin secretion. In addition, this treatment abolished phorbol ester-induced and arachidonic acid-induced insulin secretion at 3.3 mmol/l glucose. Altogether, these data suggest that sensitization of insulin secretion is caused by a preferential down-regulation of the inhibitory effects of protein kinase C, leading to an increased first phase, and an increased coupling of glucose to the stimulatory effects of protein kinase C during the second phase of glucose-induced insulin secretion. Desensitization of insulin secretion appears to be a consequence of sustained Ca2+ influx, inducing extensive down-regulation of protein kinase C and also causing deleterious effects on islet cell function in protein kinase C-deprived islets.
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