Abstract
The Antarctic notothenioid, Pagothenia bernacchii, were found to have plasma total and free calcium levels, plasma inorganic phosphate and whole body calcium efflux rates which were similar to those seen in other teleosts. But total bone calcium was lower than reported for other teleosts. A single injection of vitamin D3 (5 ng g−1 fish) increased plasma total and plasma free calcium and these increases were associated with an increase in whole body calcium efflux and bone calcification. Conversely, the same treatment with 1,25-(OH)2-D3 reduced plasma free calcium. This seco-steroid also increased the specific activity of 45Ca in bone at 40h post-injection but did not significantly effect total bone calcium, plasma total calcium or whole body calcium efflux. 25-OH-D3 at the same dose had no effect on any of the parameters tested and none of the seco-steroids tested had any effect on plasma total inorganic phosphate. These data show that both D3 and 1,25-(OH)3-D3 can have calcitropic effects in this marine teleost and that these two forms of vitamin D can exert different effects within the same species.
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References cited
Ahmad, N. and Swarup, K. 1979. Corpuscles of Stannius of Mystus vittatus in relation to calcium and sodium rich environments. Arch. Biol. 90: 1–22.
Barnett, B.J., Cho, C.Y. and Slinger, S.J. 1979. The essentiality of cholecalciferol in the diets of rainbow trout (Salmo gairdneri). Comp. Biochem. Physiol. 63A: 291–297.
Eastman, J.T. and De Vries, A.L. 1982. Buoyancy studies of Notothenioid fishes in McMurdo Sound, Antarctica. Copeia 1982: 285–393.
Fenwick, J.C. 1974. The comparative in vitro release of calcium from dried and defatted eel and rat bone fragments, and its possible significance to calcium homeostasis in teleosts. Can. J. Zool. 52: 755–764.
Fenwick, J.C. 1984. Effect of vitamin D3 (cholecalciferol) on plasma calcium and intestinal 45calcium absorption in goldfish, Carassius auratus L. Can. J. Zool. 62: 34–36.
Fenwick, J.C. 1989. Calcium exchange across fish gills. In Vertebrate Endocrinology: Fundamentals and Biomedical Implications. pp. 319–342. Edited by P.K.T. Pang and M.P. Schreibman. Academic Press, San Diego.
Fenwick, J.C. and Lam, T.J. 1988. Calcium fluxes in the teleost fish tilapia (Oreochromis) in water and in both water and air in the marble goby (Oxyeleotris) and the mudskipper (Periophthalmodon). Physiol. Zool. 61: 119–125.
Fenwick, J.C., Smith, K., Smith, J. and Flik, G. 1984. Effect of various D analogs on plasma calcium and phosphorus and intestinal calcium absorption in fed and unfed American eels, Anguilla rostrata. Gen. Comp. Endocrinol. 55: 398–404.
Flik, G., Fenwick, J.C., Kolar, Z., Mayer-Gostan, N. and Wendelaar Bonga S.E. 1986. Effects of ovine prolactin on calcium uptake and distribution in the freshwater cichlid teleost fish Oreochromis mossambicus. Am. J. Physiol. 250: R166–R173.
Halver, J.E. 1982. The vitamins required for cultivated salmonids. Comp. Biochem. Physiol. 73B: 43–50.
Henry, H. and Norman, A.W. 1975. Presence of renal 25-hydroxyvitamin-D-1-hydroxylase in species of all vertebrate classes. Comp. Biochem. Physiol. 50B: 431–434.
Holick, M.F., Holick, S.A. and Guillard, R.L. 1982. On the origin and metabolism of vitamin D in the sea. In Comparative Endocrinology of Calcium Regulation. pp. 85–91. Edited by C. Oguro and P.K.T. Pang. Japan Scientific Societies Press, Tokyo.
Hyde, D.A. and Perry, S.F. 1989. Differential approaches to blood acid-base regulation during exposure to prolonged hypercapnia in two freshwater teleosts: the rainbow trout (Salmo gairdneri) and the American eel (Anguilla rostrata). Physiol. Zool. 62: 1164–1186.
Kirsch, R. 1972. The kinetics of peripheral exchanges of water and electrolytes in the silver eel (Anguilla rostrata L.) in fresh water and sea water. J. Exp. Biol. 57: 489–512.
Lopez, E., MacIntyre, I., Martelly, E., Lallier, F. and Vidal, B. 1980. Paradoxical effect of 1,25 dihydroxycholecalciferol on osteoblastic and osteoclastic activity in the skeleton of the eel Anguilla anguilla L. Calcif. Tiss. Int. 32: 83–87.
Maetz, J. 1956. Les échanges de sodium chez le poisson Carassius auratus L. Action d'un inhibiteur de l'anhydrase carbonique. J. Physiol. 48: 1085–1099.
Perry, S.F. and Wood, C.M. 1985. Kinetics of branchial calcium uptake in the rainbow trout: effects of acclimation to various external calcium levels. J. Exp. Biol. 116: 411–433.
Poston, H.A. 1969. Effects of massive doses of vitamin D3 on fingerling brook trout. Fish. Res. Bull. N.Y. Conserv. Dep. 32: 48–50.
Sundell, K. and Björnsson, B.Th. 1990. Effects of vitamin D3, 25(OH) vitamin D3, 24,25(OH)2 vitamin D3, and 1,25-(OH)2 vitamin D3 on the in vitro intestinal calcium absorption in the marine teleost, Atlantic cod (Gadus morhua). Gen. Comp. Endocrinol. 78: 74–79.
Sundell, K., Bishop, J.E., Björnsson, B.Th., and Norman, A.W. 1992. 1,25-dihydroxyvitamin D3 in the Atlantic cod: plasma levels, a plasma binding component, and organ distribution of a high affinity receptor. Endocrinology 131: 2279–2286.
Sundell, K., Norman, A.W. and Björnsson, B.Th. 1993. 1,25(OH)2 vitamin D3 increases ionized plasma calcium concentration in the immature Atlantic cod, Gadus morhua. Gen. Comp. Endocrinol. 91: 344–351.
Swarup, K., Norman, A.W., Srivastav, A.K. and Srivastav, S.P. 1984. Dose-dependent vitamin D3 and 1,25-dihydroxyvitamin D3-induced hypercalcemia and hyperphorphatemia in male cyprinoid Cyprinus carpio. Comp. Biochem. Physiol. 78: 553–555.
Swarup, K. and Srivastav, S.P. 1982. Vitamin D3-induced hypercalcemia in male catfish, Clarias batrachus. Gen. Comp. Endocrinol. 46: 271–274.
Takeuchi, A., Okano, T., Ayame, M., Yoshikawa, H., Teraoka, S., Murakami, Y. and Kobayashi, T. 1984. High-performance liquid chromatographic determination of vitamin D3 in fish liver oils and eel body oils. J. Nutr. Sci. Viataminol. 30: 421–430.
Takeuchi, A., Okano, T. and Kobayashi, T. 1991. The existence of 25-hydroxyvitamin D3-1α-hydroxylase in the liver of carp and bastard halibut. Life Sci. 48: 275–282.
Toffaletti, J. and Bowers, G.N. Jr. 1979. The possible physiological importance of calcium complexes: An opinion. Clinica. Chimica. Acta 97: 101–105.
Urist, M.R. 1976. Biogenesis of bone: Calcium and phosphorus in the skeleton and blood in vertebrate evolution. In Handbook of Physiology Vol. 7, pp. 183–213. Edited by R.O. Greep, E.B. Astwood and G.D. Aurbach. Williams and Wilkins, Baltimore.
Wendelaar Bonga, S.E., Lammers, P.I. and Dinjens, W.N.M. 1982. Effects of calcitonin on calcium metabolism and bone formation in sea water-adapted fish Sarotherodon mossambicus. In Comparative Endocrinology of Calcium Regulation. pp. 85–91. Edited by C. Oguru and P.K.T. Pang. Japan Scientific Societies Press, Tokyo.
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Fenwick, J.C., Davison, W. & Forster, M.E. In vivo calcitropic effect of some vitamin D compounds in the marine Antarctic teleost, Pagothenia bernacchii . Fish Physiol Biochem 12, 479–484 (1994). https://doi.org/10.1007/BF00004450
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DOI: https://doi.org/10.1007/BF00004450