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  • 101
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    Pure and applied geophysics 130 (1989), S. 243-262 
    ISSN: 1420-9136
    Keywords: Mean meridional circulation ; zonally symmetric tidal theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The body force circulation problem of Eliassen is extended to spherical geometry and a quasi-compressible atmosphere using the zonally symmetric tidal theory. The concept of body force circulation is generalized to include the effects of mechanical friction and Newtonian cooling. This viewpoint is conceptually advantageous when the circulation is driven by body forces against radiative relaxation. The resulting linear theory is qualitatively useful in middle atmosphere applications, including the equatorial momentum source for which an analytic solution has not been given previously. Further generalizations of the theory are possible by including dynamical and photochemical feedback effects.
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  • 102
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    Pure and applied geophysics 130 (1989), S. 303-318 
    ISSN: 1420-9136
    Keywords: Mean circulation ; planetary waves ; mesosphere ; Southern Hemisphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract HF radar stations (utilizing the spaced-antenna partial-reflection technique) located at Adelaide (35°S, 138°E) and Mawson Station (67°S, 63°E) have observed horizontal mesospheric winds continuously since mid-1984. Observations in the period 1984–87 are compared with the Northern Hemisphere [latitude conjugate] stations of Kyoto (35°N, 136°E) and Poker Flat (65°N, 147°W), and with satellite-derived circulation models. Particular reference is made to the equinoctial changeovers in zonal flow and to the temporal and altitude variations in the planetary wave activity at Mawson and Adelaide.
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  • 103
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    Pure and applied geophysics 126 (1988), S. 141-151 
    ISSN: 1420-9136
    Keywords: atmospheric tides ; vector statistics ; lunar and solar effects ; South-west Pacific
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Lunar and solar atmospheric tidal oscillations have been determined with reasonable accuracy from a ten-year record of hourly mercury-barometer readings, corrected to mean-sea-level, at Rarotonga (Cook Islands), 21.2°S. For the lunar semidiurnal tide, the annual determination shows an amplitude (56 μb) slightly lower and a phase (51°) much smaller than the values (58 μb, 72°) that would be derived, for the position of Rarotonga, from the spherical harmonic analysis given byHaurwitz andCowley (1969). The seasonal variation of this oscillation, as given by the monthly and J, E, D values, shows most of the characteristic features found in world-wide determinations. In particular, the near equality of the J, D amplitudes at Rarotonga tends to support theHaurwitz andCowley (1969) suggestion of negative J-D values in southern middle latitudes. For the solar tides, the semidiurnal and terdiurnal oscillations at Rarotonga are similar to those found at other stations in the south-west Pacific region. However, for the diurnal oscillation, the annual amplitude (232 μb) is only about half the value (∼465 μb) indicated for the position of Rarotonga by the world maps of theS 1(p) annual harmonic coefficients given byHaurwitz (1965). It thus seems likely that the relatively small area of lowS 1(p) annual amplitude in the eastern part of the south Pacific, as indicated by these maps, is much more extensive than formerly supposed.
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  • 104
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    Pure and applied geophysics 126 (1988), S. 333-356 
    ISSN: 1420-9136
    Keywords: Earthquake prediction ; seismic quiescence ; San Andreas fault
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The Stone Canyon earthquake sequence started during August 1982 and lasted for about four months. It contained four mainshocks withM L ≥4, each with an aftershock zone about 4 km long. These mainshocks, progressing from southeast to northwest, ruptured a segment of the fault approximately 20 km long leaving two gaps, which were later filled by theM L =4.6 mainshocks of January 14, and May 31, 1986. The equivalent magnitude of the sequence isM L =5.0. Precursory seismic quiescence could be identified in: (1) the northernmost 10 km of the aftershock zone which contained three of the mainshocks; and (2) the southern gap in the aftershock zone. The fault segment containing the first mainshock and its aftershocks did not show quiescence. This pattern of precursory quiescence is very similar to two cases in Hawaii where the rupture initiation points of the mainshocks (M S =7.2 and 6.6, respectively) were located in volumes of constant seismicity rate, surrounded by volumes with pronounced precursory quiescence. The precursory quiescence before the August 1982 Stone Canyon earthquakes lasted for 76 weeks, amounted to a reduction in rate of about 60%, and could be recognized without any false alarms. That is, the anomaly was unique within the 60 km study segment of the fault and in the years 1975 through August 1982. Eighteen foreshocks occurred between July 27 and August 7, 1982. We conclude that the August 1982 mainshocks could have been predicted, based on seismic quiescence and foreshocks.
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  • 105
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    Pure and applied geophysics 126 (1988), S. 447-463 
    ISSN: 1420-9136
    Keywords: Intermediate-term precursor ; downward migration ; seismic gap ; doughnut pattern ; subduction zone ; Tonankai earthquake ; Tokachi-oki earthquake ; Tokai earthquake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Before the 1944 Tonankai earthquake along the Nankai Trough, seismic activity increased in the shallow depths, and then the activity gradually migrated downwards. When it reached its limit (a depth of approximatelty 70 km), the main shock occurred. Several deep earthquakes, including one ofM5.3, occurred several months prior to the Tonankai earthquake. A similar downward migration pattern also can be recognized regarding the 1952 Tokachi-oki earthquake. In this case the deepest earthquakes reached about 400 km. This may be one of the intermediate-term precursory phenomena of great thrusttype earthquakes in subduction zones. Recent observations in the Tokai district along the Suruga Trough, where a large earthquake is expected to occur in the future, suggest a similar downward migration pattern in the land area.
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  • 106
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    Pure and applied geophysics 126 (1988), S. 589-617 
    ISSN: 1420-9136
    Keywords: Earthquake prediction ; foreshocks ; aftershocks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Current methods for calculation of long-term probabilities for the recurrence of large earthquakes on specific fault segments are based upon models of the faulting process that implicitly assume constant stress rates during the interval separating earthquakes and instantaneous failure at a critical stress threshold. However, observations indicate that the process of stress recovery following an earthquake involves rate variations at all time scales in addition to stress steps caused by nearby earthquakes. Additionally, the existence of foreshocks, aftershocks and possible precursory processes suggest that there may be significant time dependence of the earthquake nucleation process. A method for determining the conditional probabilities for earthquake occurrence under conditions of irregular stressing is developed that could be useful at all time scales including those pertinent to short-and intermediate-term prediction. Used with models for earthquake occurrence at a stress threshold, the addition of variable stressing introduces a simple scaling of the conditional probabilities by stress level and stress rate. A model for the time-dependent nucleation of earthquake slip has been proposed recently that is based upon laboratory observations of fault strength. This failure criterion results in large but relatively short duration changes in the probability of earthquake recurrence particularly following stress steps. Applied to populations of earthquakes the models predicts a 1/t decay of seismicity following stress steps as observed for aftershocks and for frequency of foreshock-mainshock pairs. The model suggests that variations of seismicity rates of small earthquakes in the nucleation zone of the expected earthquake directly indicate variations in probability of recurrence of the large earthquake.
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  • 107
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    Pure and applied geophysics 127 (1988), S. 33-61 
    ISSN: 1420-9136
    Keywords: Seismic ; image ; reservoirs ; hydrocarbons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract This paper examines those aspects of reflection seismology which require special consideration when imaging deeper hydrocarbon reservoirs, including the constraints imposed by vertical resolution, lateral resolution, and velocity analysis. We derive quantitative expressions relating the uncertainties in stacking velocities and in interval velocities derived from stacking velocities to acquisition parameters, as well as expressions for the lateral resolution which can theoretically be achieved for migrated seismic images. This analysis shows that the most significant limitations of seismic imaging at depth involve the finite lateral resolution of the seismic method, and the proper lateral positioning of seismic images. These difficulties are overcome in large measure through the proper migration of a seismic dataset, which becomes more critical as deeper horizons are imaged. If these horizons are suspected of having significant 3-D structure, a strong argument may be made for acquiring a 3-D seismic survey over the prospect. Migration of this dataset will then generate an image of the subsurface with good lateral resolution in both the X and Y directions.
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  • 108
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    Pure and applied geophysics 131 (1989), S. 1-4 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
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  • 109
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    Pure and applied geophysics 130 (1989), S. 781-782 
    ISSN: 1420-9136
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
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  • 110
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    Pure and applied geophysics 130 (1989), S. 743-749 
    ISSN: 1420-9136
    Keywords: Dynamic systems ; multiple equilibrium ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A set of ordinary differential equations describing a mechanical system subject to forcing and dissipation is considered. A topological argument is employed to show that if all time-dependent solutions of the governing equations are bounded, the equations admitN steady solutions, whereN is a positive odd integer and where at least (N−1)/2 of the steady solutions are unstable. The results are discussed in the context of atmospheric flows, and it is shown that truncated forms of the quasigeostrophic equations of dynamic meteorology and of Budyko-Sellers climate models satisfy the hypotheses of the theorem.
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  • 111
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    Pure and applied geophysics 131 (1989), S. 77-97 
    ISSN: 1420-9136
    Keywords: Fractures ; fractal geometry ; granite permeability ; topography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Silicate melts impose systematic fracture patterns on their hosts and their own subsolidus portions as they crystallize and cool to ambient conditions. Weathering of these plutonic bodies accentuates the fractures and produces fragmented topographic surfaces whose geometric measures exceed those of a Euclidean surface. Because geometric measures of these surfaces are potentially indicative of the percolation properties of the fracture network, contours and vertical sections were digitized from 1∶250000 and 1∶240000 scale maps, and their statistical fractal dimensions,D, were computed. Regions underlain by granitic plutons with similar fragmentation patterns were found to haveD values ranging from 1.15 to 1.28 within a single pluton. This range of values is caused by noise and several geologic factors. The values contain noise introduced by the map-making and digitizing procedures that amounts toD≈1.05. However all values lie well above this threshold, and they correlate closely with local differences in weathering. SmallerD values derive from contours where accumulation of screen or glacial erosion has smoothed the surface, whereas largerD values derive from contours along aretes and in unglaciated terrains. In each of these regions, largerD values occur locally where fractures are most frequent and/or continuous. LargeD values were also derived from regions underlain by host rocks that lie in the near-field region of the stress field caused by the pluton. The fractral values derived are apparently related to the magma-hydrothermal event, they can potentially be used to derive properties of the percolation networks that were active during the hydrothermal events.
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  • 112
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    Pure and applied geophysics 131 (1989), S. 111-138 
    ISSN: 1420-9136
    Keywords: Fractals ; fractures ; fluid flow ; percolation ; rock mechanics ; geohydrology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The distributions of contact areas in single, natural fractures in quartz monzonite (Stripa granite) are found to have fractal dimensions which decrease fromD=2.00 to values nearD=1.96 as stress normal to the fractures is increased from 3 MPa up to 85 MPa. The effect of stress on fluid flow is studied in the same samples. Fluid transport through a fracture depends on two properties of the fracture void space geometry. the void aperture; and the tortuosity of the flow paths, determined through the distribution of contact area. Each of these quantities change under stress and contribute to changes observed in the flow rate. A general flow law is presented which separates these different effects. The effects of tortuosity on flow are largely governed by the proximity of the flow path distribution to a percolation threshold. A fractal model of correlated continuum percolation is presented which quantitatively reproduces the flow path geometries. The fractal dimension in this model is fit to the measured fractal dimensions of the flow systems to determine how far the flow systems are above the percolation threshold.
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  • 113
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    Pure and applied geophysics 131 (1989), S. 211-239 
    ISSN: 1420-9136
    Keywords: Fourier analysis ; fractal ; frequency spectra ; roughness ; stationarity ; numerical modeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Fractal analysis and Fourier analysis are independent techniques for quantitatively describing the variability of natural figures. Both methods have been applied to a variety of natural phenomena. Previous analytical work has formulated relationships between the fractal dimension and power law form frequency spectrum.Mandelbrot (1985) has shown that difficulties arise when the ruler method for measuring dimensionality is applied to other than self-similar figures. Since an investigator presumably does not know in advance the dimensionality of a natural profile, it is essential to quantify the nature of the discrepancy for self-affine cases. In this study, a series of experiments are conducted in which discrete random series of specified spectral forms are analyzed using the fractal ruler method. The various parameters of the fractal measurement are related to the parameters of the spectral model. In this way, empirical relationships between the techniques can be derived for discrete, finite series which simulate the results of applying the fractal method to observational data. The results of the study indicate that there are considerable discrepancies between the results predicted by theory and those derived empirically. The fundamental power law form of length versus resolution pairs does not hold over the entire region of analysis. The predicted linear relationship between fractal dimension and exponent of the frequency spectrum does not hold, and the spectral signals can be extended beyond the limits of dimension inferred by theory. Root-mean-square variability is also shown to be linearly related to the fractal intercept term. An investigation of the effect of nonstationary sampling is conducted by generating signals composed of segments of differing spectral characteristics. Fractal analyses of these signals appear identical to those conducted on stationary series. The discrepancies between theoretical prediction and empirical results described in this study reflect the difficulties of applying analytically derived techniques to measurement data. Both Fourier and fractal techniques are formulated through rigorous mathematics, assuming various conditions for the underlying signal. When these techniques are applied to discrete, finite length, nonstationary series, certain statistical transformations must be applied to the data. Methods such as windowing, prewhitening, and anti-aliasing filters have been developed over many years for use with Fourier analysis. At present, no such statistical theory exists for use with fractal analysis. It is apparent from the results of this study that such a statistical foundation is required before the fractal ruler method can be routinely applied to observational data.
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  • 114
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    Pure and applied geophysics 131 (1989), S. 255-271 
    ISSN: 1420-9136
    Keywords: Fractals ; fault-gouge ; friction ; faulting ; stick-slip ; velocity-weakening ; self-similarity ; fractal dimension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The particle-size distribution of a natural fault-gouge has been determined over the range from 5 μm to 40 cm. The gouge is self-similar over the range from 5 μm to 1 cm having a fractal dimension of 2.60±0.11. The lower and upper fractal limits were also determined. The lower fractal limit occurs at a dimension of about 1–10 μm where mineral cleavage and intergranular porosity dominate the cataclasis. The upper fractal limit occurs at particle sizes on the order of 1 cm where the scaled particle density decreases abruptly by a factor of about three. By analogy to soil-mechanics shear box tests and laboratory rock friction experiments, it is argued that the upper fractal limit of the gouge determines the characteristic displacement parameter in stick-slip friction models. A characteristic displacement on the order of 1 cm is consistent with estimates based on numerical simulations of faulting.
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  • 115
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    Pure and applied geophysics 131 (1989), S. 333-341 
    ISSN: 1420-9136
    Keywords: Quiet day ; geomagnetic indices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Based on published literature and the response to a questionnaire sent to geomagnetic field, ionospheric and magnetospheric researchers, several methods of choosing periods of quiet conditions based on geomagnetic records, as well as other observed parameters, have been identified. Caveats with respect to using geomagnetic indices to select quiet periods include the following: 1. Geomagnetic disturbances are strongly local. Even if the data from all available observatories indicate quiet behavior, there is the distinct possibility that some other location, not sampled, may be disturbed. 2. Geomagnetic indices are convenient but imperfect indicators of geomagnetic activity. Indices based on a quiet-day reference level have uncertainties comparable to the threshold value for quiet conditions. Indices representing average conditions during a 24-hr UT day may not be appropriate. 3. Geomagnetic activity does not fully reflect the range of possible factors that influence the ionosphere or magnetosphere.
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  • 116
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    Pure and applied geophysics 131 (1989), S. 357-370 
    ISSN: 1420-9136
    Keywords: Spherical harmonic analysis ; Sq
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Spherical harmonic coefficients (SHCs) for the daily magnetic variation fields (solar and lunar) and the main field of the earth are usually estimated by the method of least squares applied to a truncated spherical harmonic series. In this paper, an integral method for computing the SHCs for the solar quiet daily magnetic variation fieldSq is described and applied toSq data for May and June 1965. TheSq SHCs thus derived are then compared with the results obtained using both unweighted and weighted versions of the least squares method. The weighting used tends to orthogonalize the least squares terms. The integral and weighted least squares results agree closely for terms up to order 4 and degree 30, but both disagree considerably for the higher degree terms with the results of the unweighted least squares. Errors introduced by the numerical integration can be shown to be small, hence the disagreement between integral and unweighted least squares coefficient sets arises from improper weighting. Also, it is concluded that discrepancies between the geomagnetic northward and eastward component-derived coefficient sets arise from either time-dependent external sources that produce non-local-time, based fields or nonpotential sources and not from truncation of the spherical harmonic series as has previously been suggested.
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  • 117
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    Pure and applied geophysics 131 (1989), S. 447-462 
    ISSN: 1420-9136
    Keywords: Magnetospheric currents ; Sq
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Since the discovery of the magnetosphere, it has been known that the currents flowing in the magnetosphere contribute toSq, the regular daily variation in the earth's surface magnetic field. The early models, however, were not very accurate in the vicinity of the earth. The magnetospheric contribution toSq has therefore been recalculated by direct integration over the three major magnetospheric current systems; magnetopause, tail and ring. The finite electrical conductivity of the earth, which increases the horizontal and decreases the vertical components of the magnetospheric field at the earth's surface, has been taken into account. The magnetospheric currents are found to contribute 12 nanotesla to the day to night difference in the mid-latitudeSq pattern for steady, quiet magnetospheric conditions. They also contribute to the annual variation in the surface field and must be considered an important source of the observed day to day variation in theSq pattern.
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  • 118
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    Pure and applied geophysics 131 (1989), S. 527-531 
    ISSN: 1420-9136
    Keywords: Lunar geomagnetic variations ; L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract A brief description of existing methods of determiningL of geophysical elements is given in this paper. Their evolution or various modifications are briefly mentioned. It is hoped that scientific studies of lunar perturbations in geophysics and aeronomy should be encouraged and strengthened during future years.
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  • 119
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    Pure and applied geophysics 131 (1989), S. 577-603 
    ISSN: 1420-9136
    Keywords: Elastic wave ; random media ; multiple scattering ; phase velocity and attenuation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In seismic exploration, elastic waves are sent to investigate subsurface geology. However, the transmission and interpretation of the elastic wave propagation is complicated by various factors. One major reason is that the earth can be a very complex medium. Nevertheless, in this paper, we model some terrestrial material as an elastic medium consisting of randomly distributed inclusions with a considerable concentration. The waves incident on such an inhomogeneous medium undergo multiple scattering due to the presence of inclusions. Consequently, the wave energy is redistributed thereby reducing the amplitude of the coherent wave. The coherent or average wave is assumed to be propagating in a homogeneous continuum characterized by a bulk complex wavenumber. This wavenumber depends on the frequency of the probing waves; and on the physical properties and the concentration of discrete scatterers, causing the effective medium to be dispersive. With the help of multiple scattering theory, we are able to analytically predict the attenuation of the transmitted wave intensity as well as the dispersion of the phase velocity. These two sets of data are valuable to the study of the inverse scattering problems in seismology. Some numerical results are presented and also compared, if possible, with experimental measurements.
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  • 120
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    Pure and applied geophysics 131 (1989), S. 715-739 
    ISSN: 1420-9136
    Keywords: Inverse scattering ; elastic wave ; nondestructive evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Ultrasonic detection and characterization of flaws in metals and ceramics is of considerable technological interest. Scattering and inverse scattering theories have recently been applied to these tasks in a systematic manner and considerable progress has resulted. This paper first reviews briefly the development of scattering and inverse scattering methods in the AF/DARPA Program in Quantitative Nondestructive Evaluation.2 Then one particular inverse method studied in that program, the inverse Born approximation, is discussed in detail. Progress is reviewed and the ability of the method to distinguish volumetric and crack-like flaws is demonstrated in simple cases.
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  • 121
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    Pure and applied geophysics 126 (1988), S. 373-406 
    ISSN: 1420-9136
    Keywords: Earthquake prediction ; quiescence ; seismic quiescence ; statistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Numerous cases of precursory seismic quiescence have been reported in recent years. Some investigators have interpreted these observations as evidence that seismic quiescence is a somewhat reliable precursor to moderate or large earthquakes. However, because failures of the pattern to predict earthquakes may not, in general, be reported, and because numerous earthquakes are not preceded by quiescence, the validity and reliability of the quiescence precursor have not been established. We have analyzed the seismicity rate prior to, and in the source region of, 37 shallow earthquakes (M 5.3–7.0) in central California and Japan for patterns of rate fluctuation, especially precursory quiescence. Nonuniformity in rate for these pre-mainshock sequences is relatively high, and numerous intervals with significant (p〈0.10) extrema in rate are observed in some of the sequences. In other sequences, however, the rate remains within normal limits up to the time of the mainshock. Overall, in terms of an observational basis for intermediate-term earthquake prediction, no evidence is found in the cases studied for a systematic, widespread or reliable pattern of quiescence prior to the mainshocks. In earthquake sequences comprising full seismic cycles for 5 sets of (M 3.7–5.1) repeat earthquakes on the San Andreas fault near Bear Valley, California, the seismicity rates are found to be uniform. A composite of the estimated rate fluctuations for the sequences, normalized to the length of the seismic cycle, reveals a weak pattern of a low rate in the first third of the cycle, and a high rate in the last few months. While these observations are qualitative, they may represent weak expressions of physical processes occurring in the source region over the seismic cycle. Re-examination of seismicity rate fluctuations in volumes along the creeping section of the San Andreas fault specified by Wyss and Burford (1985) qualitatively confirms the existence of low-rate intervals in volumes 361, 386, 382, 372 and 401. However, only the quiescence in volume 386 is found by the present study to be statistically significant.
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  • 122
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    Pure and applied geophysics 127 (1988), S. 93-115 
    ISSN: 1420-9136
    Keywords: Water vapour ; atmospheric models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Two sets of radiosounding measurements, taken at different hours from some stations in the Po Valley area, were examined in order to obtain the values of precipitable waterw and determine the shapes of the vertical distribution curves of absolute humidity. From these curves, we calculated the bestfit values of scale heightsH 1 andH 2 in the 0÷5 km and 5÷10 km altitude intervals, respectively. The analysis of the radiosounding data clearly shows that parametersw andH 1 are closely related to the evolutionary features of the meteorological conditions on synoptic scale and are influenced, to a lesser extent, by the diurnal variations in the atmospheric ground layer. Seasonal average curves of temperature and absolute humidity, as functions of altitude, were also defined from the radiosounding measurements taken at various hours of the day. Moreover, interpolation methods in time (applied to a 12-hour range) and in space (range of about 300 km) were proposed for determining parametersw andH 1 from the radiosounding measurements taken at different hours from the same station or from different stations at the same hour. Reliability tests, made by comparing the same station or from different stations at the same hour. Reliability tests, made by comparing the values given by the interpolation methods with those directly obtained from the radiosounding measurements, show that these evaluations ofw andH 1 are affected by standard errors of estimate, which are comparable to the errors usually made in analyzing the radiosounding data.
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  • 123
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    Pure and applied geophysics 127 (1988), S. 155-160 
    ISSN: 1420-9136
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  • 124
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  • 125
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    Pure and applied geophysics 127 (1988), S. 447-471 
    ISSN: 1420-9136
    Keywords: Interhemispheric coupling ; ionosphere ; plasmasphere ; nighttime winter anomaly ; thermal ion fluxes ; numerical modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The nighttime winter anomaly (NWA) effect was observed during solar minimum conditions at the American sector by means of ionospheric electron content and vertical sounding measurements in Havana (Cuba). An effective interhemispheric transport of plasma is suggested to explain enhanced northern nighttime ionization during winter solstice. To elucidate this effect, an adequate physicalnumerical model of the coupled system ionosphere-plasmasphere is presented and applied to a corotating tube of plasma at L=1.5 in the American sector. The NWA can be explained by theoretically derived higher tube content during the December solstice and accordingly by more intense nighttime fluxes from the plasmasphere, compared to the June solstice.
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  • 126
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    Pure and applied geophysics 130 (1989), S. 181-194 
    ISSN: 1420-9136
    Keywords: Southern Hemisphere stratosphere ; sensitivity of stratospheric circulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The impact of different base-level analyses on derived stratospheric circulation statistics for the Southern Hemisphere has been assessed. Three different sets of daily operational analyses of geopotential height at 100 hPa for September, 1981 have been used as the base-level analyses, combined with a single set of daily thickness analyses for the stratosphere. The circulation statistics considered include mean fields, transient eddy statistics, Eliassen-Palm flux diagnostics and vorticity fields. In general, the different base-level analyses do not change the qualitative description of the circulation statistics but they lead to marked quantitative differences, particularly at high latitudes. The statistics which are most sensitive to the different base-level analyses are those which emphasise the shortest space scales through multiple differentiation of the height field and the shortest time scales, such as daily Eliassen-Palm flux diagrams or the daily vorticity fields.
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  • 127
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    Pure and applied geophysics 127 (1988), S. 607-625 
    ISSN: 1420-9136
    Keywords: Physical properties of rocks ; permeability
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    Topics: Geosciences , Physics
    Notes: Abstract Permeability, resistivity formation factor, and pore volume change were simultaneously measured on samples of Chelmsford granite subjected to confining pressure and pore pressure cycles. Using a technique described in a previous paper, the tangent coefficients of the effective pressure law for permeability α k and for formation factor α F were determined. α k and α F did not differ significantly from one another. They showed a strong stress history dependence as has already been observed for α k in several crystalline rocks. According to the definition of the effective pressure law used here, two physical properties with identical α's must be related through a one-to-one functional relationship. Hence, the observation above suggests that such a relationship may be empirically found between permeability and formation factor. Indeed, analysis of the data revealed that, to a good approximation, permeability was inversely proportional to the formation factor. The same relation has previously been observed in other crystalline rocks. This relationship was included in a recent version of the so-called equivalent channel model. Using this model, the specific surface area of the cracksA c/VS, the standard deviation of the distribution of asperities heightsh and the hydraulic radiusm o were evaluated. The following values were respectively found: 850 cm−1, 0.008 μm and 0.14 μm. The specific surface area of the cracks was independently estimated on micrographs of polished sections using a standard quantitative stereology method. The result was in good agreement with the values estimated from the transport properties data.
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  • 128
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    Pure and applied geophysics 130 (1989), S. 343-371 
    ISSN: 1420-9136
    Keywords: Gravity waves ; saturation ; middle atmosphere dynamics ; turbulence ; diffusion ; momentum fluxes
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    Topics: Geosciences , Physics
    Notes: Abstract This paper provides a review of our current understanding of the processes responsible for gravity wave saturation as well as the principal effects and variability of saturation in the lower and middle atmosphere. We discuss the theoretical and observational evidence for linear and nonlinear saturation processes and examine the consequences of saturation for wave amplitude limits, momentum and energy fluxes, the diffusion of heat and constituents, and the establishment of a near-universal vertical wavenumber spectrum. Recent studies of gravity wave variability are reviewed and are seen to provide insights into the significant causes of wave variability throughout the atmosphere.
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  • 129
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    Pure and applied geophysics 130 (1989), S. 399-420 
    ISSN: 1420-9136
    Keywords: Gravity waves ; saturation ; wave breaking ; energy dissipation ; momentum flux ; mean flow acceleration
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    Topics: Geosciences , Physics
    Notes: Abstract In this paper we consider a vertical wavenumber spectrum of vertically propagating gravity waves impinging on a rapid increase in atmospheric stability. If the high-wavenumber range is saturated below the increase, as is usually observed, then the compression of vertical scales as the waves enter a region of higher stability results in that range becoming supersaturated, that is, the spectral amplitude becomes larger than the saturation limit. The supersaturated wave energy must then dissipate in a vertical distance of the order of a wavelength, resulting in an enhanced turbulent energy dissipation rate. If the wave spectrum is azimuthally anisotropic, the dissipation also results in an enhanced vertical divergence of the vertical flux of horizontal momentum and enhanced wave drag in the same region. Estimates of the enhanced dissipation rates and radar reflectivities appear to be consistent with the enhancements observed near the high-latitude summer mesopause. Estimates of the enhanced mean flow acceleration appear to be consistent with the wave drag that is needed near the tropopause and the high-latitude summer mesopause in large-scale models of the atmosphere. Thus, this process may play a significant role in determining the global effects of gravity waves on the large-scale circulation.
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  • 130
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    Keywords: MST radar observation ; cold vortex ; tropopause funnel ; internal gravity waves
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    Topics: Geosciences , Physics
    Notes: Abstract Vertical and temporal variations of three-dimensional wind velocity associated with an upper-tropospheric cold vortex-tropopause funnel system were observed by an MST radar in Japan (the MU radar). Marked changes of vertical velocity and horizontal wind direction were found between the inside and outside of the cold vortex. The vertical velocity activity outside the vortex was asymmetric; it was most active in a sector before the vortex. Unsaturated internal gravity waves in their generation stage contribute predominantly to the vertical velocity activity, suggesting that tropospheric occluded cyclones may be a possible source of middle-atmospheric gravity waves through the geostrophic adjustment process.
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  • 131
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    Pure and applied geophysics 129 (1989), S. 325-343 
    ISSN: 1420-9136
    Keywords: Source mechanism ; seismic moment tensor ; gas outburst ; Sunagawa Coal Mine
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    Topics: Geosciences , Physics
    Notes: Abstract On January 29ty, 1986, the third largest gas outburst in Japan took place at Sunagawa Coal Mine, which is the only hydraulic mine in Japan. It occurred at a face of a cross-cut, just after a coal seam was outcropped by blasting for drivage of the cross-cut. The site of the gas outburst was located 1,180 m below the surface. No workers were injured, but the cross-cut was plugged with 1,600 m3 of coal fragments extending over 100 m behind the face and 60,000 m3 of methane gas was emitted. The site of the gas outburst was investigated in detail to clarify the geological features. A normal and a reverse fault existed at the site. The area of the ejected zone was about 400 m2 and extended upward along the normal fault. The shape of the ejected zone suggests a great role of the normal fault on the gas outburst. Digital seismograms, recorded by a mine-wide seismic array at the coal mine, consisting of 27 microseismic events were used to investigate the gas outburst. Magnitude, seismic energy release, distribution of hypocenter and focal mechanism were analyzed. Taking the shape of the ejected zone together with results of the seismological investigation into consideration, it appears that the seismicity started with left-lateral faulting of the reverse fault and then right-lateral faulting of the normal fault followed. The faulting of the normal fault might be the direct cause and be the predominant mechanism of the gas outburst.
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  • 132
    ISSN: 1420-9136
    Keywords: MU radar ; mesosphere ; turbulence ; scattering layer ; full-correlation analysis technique ; gravity wave ; wave breaking
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    Notes: Abstract We have applied a full-correlation analysis technique to the echo power fluctuations observed by the MU radar (35°N, 136°E), and analyzed the horizontal structure of the scattering pattern in the mesosphere as well as their horizontal motions. The velocity of the scattering pattern did not agree with the background wind velocity, but was associated with the horizontal propagating direction of a saturated inertia gravity wave identified in the wind field. The length of the long axis of the characteristic ellipse of the scattering pattern was approximately 50 km, and the direction was almost perpendicular to the propagating direction of the wave. The correlation time of the scattering pattern was approximately 700 s, which is much longer than the lifetime of the isolated turbulence itself. This implies that the observed scattering pattern is associated with a region where the saturated inertia gravity wave generates turbulence.
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  • 133
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    Pure and applied geophysics 130 (1989), S. 687-697 
    ISSN: 1420-9136
    Keywords: Thermal conductivity ; transient heat transfer ; borehole temperature logging
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    Topics: Geosciences , Physics
    Notes: Abstract A temperature-transient method to estimatein situ formation thermal conductivity and equilibrium formation temperature using circulation fluid flow-rate pulses is described. Flow-rate changes induce temperature variations in the well and the time from when the flow alters to the subsequent maximum or minimum temperature of the drilling fluid can be used to determine these two formation parameters. Some examples are presented to illustrate the method.
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  • 134
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    Pure and applied geophysics 131 (1989), S. 5-42 
    ISSN: 1420-9136
    Keywords: Fractal ; multifractal ; measure ; Hölder ; limit theorem
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    Notes: Abstract This text is addressed to both the beginner and the seasoned professional, geology being used as the main but not the sole illustration. The goal is to present an alternative approach to multifractals, extending and streamlining the original approach inMandelbrot (1974). The generalization from fractalsets to multifractalmeasures involves the passage from geometric objects that are characterized primarily by one number, namely a fractal dimension, to geometric objects that are characterized primarily by a function. The best is to choose the function ϱ(α), which is a limit probability distribution that has been plotted suitably, on double logarithmic scales. The quantity α is called Hölder exponent. In terms of the alternative functionf(α) used in the approach of Frisch-Parisi and of Halseyet al., one has ϱ(α)=f(α)−E for measures supported by the Euclidean space of dimensionE. Whenf(α)≥0,f(α) is a fractal dimension. However, one may havef(α)〈0, in which case α is called “latent.” One may even have α〈0, in which case α is called “virtual.” These anomalies' implications are explored, and experiments are suggested. Of central concern in this paper is the study of low-dimensional cuts through high-dimensional multifractals. This introduces a quantityD q, which is shown forq〉1 to be a critical dimension for the cuts. An “enhanced multifractal diagram” is drawn, includingf(α), a function called τ(q) andD q.
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  • 135
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    Pure and applied geophysics 131 (1989), S. 157-170 
    ISSN: 1420-9136
    Keywords: Fracture geometry ; fractal dimension ; self-similarity
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    Notes: Abstract Based on fault maps, whether or not the fracture geometry of rocks is self-similar, was examined by using a box-counting algorithm. The statistical self-similarity (fractal structure) of the fault fracture systems holds well at the scale of about 2 to 20 km. The fractal dimension in Japan varied from 1.05 to 1.60. The fractal dimension is about 1.5–1.6 at the central part of the Japan Arc, and decreases with distance from the center. At a smaller scale, the fractal structure also holds well in the rock fracture geometry. The fractal dimension of the North Izu Peninsula fault system (branching faults) is 1.49 at the scale of 0.625 to 10 km, the fractal dimension of rock fracture geometry at the scale order of 10−1 to 10−2 meters is about 1.49–1.61. The upper limit of the fractal dimension of rock fracture geometry is about 1.6, judging from the estimation of fractal dimension on actual fracture geometry of rocks. This value may impose a restraint on modeling of faulting and the fracture process of rocks.
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  • 136
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    Pure and applied geophysics 130 (1989), S. 1-3 
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  • 137
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    Pure and applied geophysics 130 (1989), S. 83-97 
    ISSN: 1420-9136
    Keywords: macroseismic data ; focal mechanism ; circular source ; energy flow ; directivity ; local effects
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    Topics: Geosciences , Physics
    Notes: Abstract A simple method is presented for the computation of theoretical models of the macroseismic field, approximately valid close to the epicentre of a weak crustal earthquake. It is assumed that the intensity is logarithmically proportional to the energy flux of a complete directS wave. A circular source is used, whose energy-flux directivity is weak and thus simply predictable. The focal mechanism influences the solution through standard far-field double-couple radiation patterns. For the wave propagation in the layered crust the ray method is used, and a simple absorption correction is applied. Conversion coefficients at the earth's surface are included. To speed up repeated computations of the theoretical macroseismic fields for varying focal mechanisms, the ray quantities are computed (and stored) separately. This makes the program fast and simple enough even for routine applications on small microcomputers, whenever observed macroseismic fields, focal mechanisms, and hypocentre locations need joint interpretation.
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  • 138
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    Pure and applied geophysics 130 (1989), S. 151-170 
    ISSN: 1420-9136
    Keywords: Atmospheric dynamics ; atmospheric waves ; gravity waves ; upper atmosphere ; history of atmospheric science
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    Notes: Abstract The means whereby the author came to be involved in the study of atmospheric gravity waves, and then came to involve others in that study, are outlined. In particular, events leading up to, during and following the International Symposium on Fluid Mechanics in the Ionosphere, of July 1959, are described.
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  • 139
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    Pure and applied geophysics 130 (1989), S. 291-301 
    ISSN: 1420-9136
    Keywords: Mesosphere ; high latitude ; Southern Hemisphere ; partial reflection radar ; winds
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    Topics: Geosciences , Physics
    Notes: Abstract A medium frequency partial-reflection spaced-antenna wind radar was installed at Scott Base (78S) on Ross Island, Antarctica, in November 1982. Results from this radar for the period December 1982 to October 1984 inclusive are compared with simultaneous measurements made with a similar radar at Christchurch (44S), N. Z. Monthly mean zonal winds measured at 80 km are compared with recent models for the Southern Hemisphere middle atmosphere. There is a general agreement with the models but there is evidence that the Christchurch winter flow was atypical in 1983.
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  • 140
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    Pure and applied geophysics 130 (1989), S. 373-397 
    ISSN: 1420-9136
    Keywords: Internal gravity waves ; nonlinear instability ; transport
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    Topics: Geosciences , Physics
    Notes: Abstract Gravity wave saturation is an important process affecting the transport and deposition of momentum, heat, and constituents in the earth's atmosphere. This paper informally discusses several saturation mechanisms and their effects, including convection, Kelvin-Helmholtz instability, vortical mode instability, parametric subharmonic instability, and mean flow interaction. Convective saturation is emphasized. The parameterization of convective adjustment is discussed and a few remarks are made concerning the effects of turbulence localization on the convective saturation process. Several outstanding problems in saturation theory are identified that could be addressed with observational, numerical, and laboratory studies.
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  • 141
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    Pure and applied geophysics 128 (1988), S. 101-118 
    ISSN: 1420-9136
    Keywords: Reflection ; transmission ; plane waves ; compact inhomogeneities ; cracks
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    Topics: Geosciences , Physics
    Notes: Abstract Reflection and transmission of elastic wave motion by a layer of compact inhomogeneities has been analyzed. For identical inhomogeneities whose geometrical centers are periodically spaced, the problem has been formulated and solved rigorously. The reflected and transmitted longitudinal and transverse wave motions have been expressed as superpositions of wavemodes, where each wavemode has its own cut-off frequency. At its cut-off frequency a mode converts from a standing into a propagating wavemode. The standing wavemodes decay exponentially with distance to the plane of the centers of the inhomogeneities. At small frequencies only the lowest order modes of longitudinal and transverse wave motion are propagating. Reflection and transmission coefficients have been defined in terms of the coefficients of the zeroth-order scattered wavemodes. These coefficients have been computed by a novel application of the Betti-Rayleigh reciprocal theorem. They are expressed as integrals over the surface of a single inhomogeneity, in terms of the displacements and tractions on the surface of the inhomogeneity. The system of singular integral equations for the surface fields has been solved numerically by the boundary integral equation method. Curves show the reflection and transmission coefficients for the reflected and transmitted longitudinal and transverse waves as functions of the frequency. Some results are also presented for planar distributions of cracks whose spacing and size are random variables. Finally, dispersion relations are discussed for solids which are completely filled with periodically spaced inhomogeneities.
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  • 142
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    Keywords: Separation of scattering and absorption ; multiple scattering ; coda envelopes ; crustal heterogeneities
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    Notes: Abstract In order to separate the scattering effect from intrinsic attenuation, we need a multiple scattering model for seismic wave propagation in random heterogeneous media. In paper I (Wu, 1985), radiative transfer theory is applied to seismic wave propagation and the energy density distribution (or the average intensity) in space for a point source is formulated in the frequency domain. It is possible to separate the scattering effect and the absorption based on the measured energy density distribution curves. In this paper, the data from digital recordings in the Hindu Kush region are used as an example of application of the theory. We also discuss two approximate solutions of coda envelope in the time domain: the single scattering approximation and the diffusion approximation and discuss the relation with the frequency domain solution. We point out that in only two cases can the apparent attenuation be expressed as an exponential decay form. One is thedark medium case, i.e., whenB 0≪0.5, whereB 0 =η s /(η s +η a ) is the seismic albedo,η s is the scattering coefficient,η a is the absorption coefficient. In this case the absorption is dominant, the apparent attenuationb can be approximated by the coherent wave attenuationb =η s +η a . The other case is thediffuse scattering regime, i.e., whenB 0≫0.5 (bright medium) andR≫L s ,t ≪ τ s , whereR andt are the propagation distance and lapse time,L s and τ s are the scattering lengths (mean free path) and scattering time (mean free time), respectively. However, in this case the envelope decays with a rate close to the intrinsic attenuation, while the intensity decreases with distance with a coefficientb ≈d 0(η s +η a ) ≈d s η s , whered 0 andd s are the diffusion multipliers (0〈d 0,d s 〈1). For the Hindu Kush region, by comparing the theory with data from two digital stations of 53 events distributed up to depths of 350 km, we find that the scattering is not the dominant factor for the measured apparent attenuation ofS waves in the frequency range 2–20 Hz. From the observation on high frequency (f〉20 Hz) seismograms, we suggest the existence of a stron-scattering surface layer with fine scale heterogeneities in the crust, at least for this region.
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  • 143
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    Pure and applied geophysics 128 (1988), S. 157-193 
    ISSN: 1420-9136
    Keywords: Scattering ; finite difference ; Kirchhoff integration ; NTS
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    Topics: Geosciences , Physics
    Notes: Abstract A new method for interfacing numerical and integral techniques allows greater flexibility in seismic modeling. Specifically, numerical calculations in laterally varying structure are interfaced with analytic methods that enable propagation to great distances. Such modeling is important for studying situations containing localized complex regions not easily handled by analytic means. The calculations involved are entirely two-dimensional, but the use of an appropriate source in combination with a filter applied to the resulting seismograms produces synthetic seismograms which are point-source responses in three dimensions. The integral technique is called two-dimensional Kirchhoff because its form is similar to the classical three-dimensional Kirchhoff. Data from Yucca Flat at the Nevada Test Site are modeled as a demonstration of the usefulness of the new method. In this application, both local and teleseismic records are modeled simultaneously from the same model with the same finite-difference run. This application indicates the importance of locally scattered Rayleigh waves in the production of teleseismic body-wave complexity and coda.
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  • 144
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    Pure and applied geophysics 128 (1988), S. 231-249 
    ISSN: 1420-9136
    Keywords: Scattering ; coda ; underground explosions ; yield estimation ; wave propagation
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    Topics: Geosciences , Physics
    Notes: Abstract The earlyP wave coda (5–15 sec after the first arrival) of underground explosions at the Nevada Test Site is studied in the time domain using 2082 teleseismic short-period recordings, with the intent of identifying near-source contributions to the signals in the frequency range 0.2–2.0 Hz. Smaller magnitude events tend to have relatively high coda levels in the 0.4–0.8 Hz frequency band for both Yucca Flat and Pahute Mesa explosions. Coda complexity in this low-frequency passband is negatively correlated with burial depth for Pahute Mesa events but is only weakly correlated with depth for Yucca Flat events. Enhanced excitation of relatively long-period scattered waves for smaller, less deeply buried events is required to explain this behavior. Coda complexity in the 0.8–1.1 Hz band is positively correlated with magnitude and depth for Pahute Mesa events, but has no such dependence for Yucca Flat events. This may result from systematic variations between the spectra of direct signals and coda arrivals caused bypP interference for the largest events, all of which were detonated at Pahute Mesa. Another possible explanation is a frequency-dependent propagation effect on the direct signals of the larger events, most of which were located in the center of the mesa overlying strong lateral velocity gradients in the crust and upper mantle. Event average complexity varies spatially for both test sites, particularly in the 0.8–1.1 Hz band, providing evidence for frequency-dependent focussing or scattering by near-source structure. Both the direct arrivals and the early coda have strong azimuthal amplitude patterns that are produced by defocussing by mantle heterogeneity. The direct arrivals have stronger coherent azimuthal patterns than the early coda for Pahute Mesa events, indicating more pronounced deep crustal and shallow mantle defocussing for the direct signals. However, for Yucca Flat events the direct arrivals have less coherent azimuthal patterns than the coda, suggesting that a highly variable component of near-source scattering preferentially affecting the downgoing energy is superimposed on a pattern produced by mantle heterogeneity that affects the entire signal. This complicated behavior of the direct arrivals may be the result of triplications and caustics produced by the complex basement structure known to underlie the Yucca Flat test site. The presence of strong azimuthal patterns in the early coda indicates that source strength estimates based on early coda are subject to biases similar to those affecting estimates based on direct arrivals.
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  • 145
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    Keywords: Autoregressive modeling ; nonstationary time-frequency variations ; seismic-Q ; coda waves
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    Notes: Abstract A technique to detect spectrum variations versus time along seismic signals is applied to coda waves of local earthquakes (Friuli, Northern Italy). The technique consists of an autoregressive modeling and utilizes nonlinear spectral analysis where the spectrum of stochastic processes is estimated as the transfer function of the filter that whitens the process under analysis. This approach appears to be particularly well suited to those investigations where automatic measurements of the instantaneous frequency have to be carried out on digital data. The detection of variations of the instantaneous frequency along the coda allows computation of seismic-Q in the lithosphere and its frequency dependence: the result obtained is $$Q = 100f^{0.4} $$ which appears to be strongly consistent with that, based on the estimate of the coda amplitude decay in the band including the most significant frequencies of the signals under analysis.
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  • 146
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    Pure and applied geophysics 128 (1988), S. 333-363 
    ISSN: 1420-9136
    Keywords: Information content ; high-frequency seismograms ; hard rock ; f max ; site resonance ; fractures
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    Topics: Geosciences , Physics
    Notes: Abstract Due to hardware developments in the last decade, the high-frequency end of the frequency band of seismic waves analyzed for source mechanisms has been extended into the audio-frequency range (〉20 Hz). In principle, the short wavelengths corresponding to these frequencies can provide information about the details of seismic sources, but in fact, much of the “signal” is the site response of the nearsurface. Several examples of waveform data recorded at “hard rock” sites, which are generally assumed to have a “flat” transfer function, are presented to demonstrate the severe signal distortions, includingf max, produced by near-surface structures. Analysis of the geology of a number of sites indicates that the overall attenuation of high-frequency (〉1 Hz) seismic waves is controlled by the whole-path-Q between source and receiver but the presence of distinctf max site resonance peaks is controlled by the nature of the surface layer and the underlying near-surface structure. Models of vertical decoupling of the surface and nearsurface and horizontal decoupling of adjacent sites on hard rock outcrops are proposed and their behaviour is compared to the observations of hard rock site response. The upper bound to the frequency band of the seismic waves that contain significant source information which can be deconvolved from a site response or an array response is discussed in terms off max and the correlation of waveform distortion with the outcrop-scale geologic structure of hard rock sites. It is concluded that although the velocity structures of hard rock sites, unlike those of alluvium sites, allow some audio-frequency seismic energy to propagate to the surface, the resulting signals are a highly distorted, limited subset of the source spectra.
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  • 147
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    Pure and applied geophysics 128 (1988), S. 423-432 
    ISSN: 1420-9136
    Keywords: Seismic attenuation ; porous media ; permeability ; local flow
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    Topics: Geosciences , Physics
    Notes: Abstract Contrary to the traditional view, seismic attenuation in Biot's theory of fluid-saturated porous media is due to viscous damping of local (not global) pore-fluid motion. Since substantial inhomogeneities in fluid permeability of porous geological materials are to be expected, the regions of highest local permeability contribute most to the wave energy dissipation while those of lowest permeability dominate the fluid flow rate if they are uniformly distributed. This dichotomy can explain some of the observed discrepancies between computed and measured attenuation of compressional and shear waves in porous earth. One unfortunate consequence of this result is the fact that measured seismic wave attenuation in fluid-filled geological materials cannot be used directly as a diagnostic of the global fluid-flow permeability.
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  • 148
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    Keywords: Attenuation ; spectral ratio ; peridotite ; partial melting ; high pressure and temperature
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    Notes: Abstract A technique has been developed to determine attenuation in rocks at high temperature using a gas-media, high-pressure apparatus. A pulse transmission technique and a spectral ratio method are used to study compressional seismic properties of rocks. Seismic waves are transmitted to and from the sample through buffer rods of mullite. The effect of seismic wave reflections within the sample assembly are cancelled out by taking ratios of the spectra measured at different temperatures. In order to obtain good signal-to-noise ratio for resolving the attenuation at high pressure and temperature, special care is taken in the sample assembly and the ultrasonic coupling between the sample, buffer rods and transducers. A very tight connection of the sample-buffer rod-transducer is essential for obtaining high frequency signals (〉300 kHz) at high temperature. A small mass is attached to each outside end of the transducer to drive low frequency signals (〈250 kHz) into the sample. Before attenuation measurements, the sample and the buffer rods are tightly compacted in a platinum tube at high pressure and room temperature to ensure pressure seal of the sample assembly. The frequency range of measurement covers 50 to 450 kHz for the sample. Attenuation is very small in the buffer rod compared to the sample for the entire temperature range of the study. Because of the small attenuation, a wide frequency band of 50 kHz to 3.2 MHz can be covered for investigating the attenuation in the buffer rod. The technique has been used to measure attenuation at high confining pressure, and temperatures including sub- and hyper-solidus of upper mantle rocks. Therefore, effects of partial melting on attenuation can be studied. The method is applied to the attenuation measurement in a peridotite as a function of temperature to 1225°C at 200 MPa confining pressure. At high temperature, signal amplitude decays more rapidly at high frequency than at low frequency, from which attenuation (andQ) can be determined using a spectral ratio method. No frequency dependence ofQ is resolved for both the sample and the buffer rod over the entire temperature and frequency ranges of the measurement. The results show thatQ decreases rapidly with increasing temperature even in the temperature range below the solidus of peridotites. Such temperature sensitivity ofQ is probably more useful to probe thermal structure in the upper mantle than that of conductivity at temperatures below the solidus. The results in this study are compared with available seismic velocity, electrical conductivity and solidus data for peridotites, suggesting that there is no discontinuous change in both mechanical and electrical properties of peridotites at the solidus temperature. Even at hypersolidus temperatures, it appears that velocity drops and conductivity increases continuously (not abruptly) with increasing melt fraction. This implies that mechanical and electrical properties of the upper mantle will gradually change at the boundary where the geotherm crosses the solidus.
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  • 149
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    Pure and applied geophysics 128 (1988), S. 547-624 
    ISSN: 1420-9136
    Keywords: Geodynamics ; seismotectonics ; global seismicity ; subduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Summary The pattern of seismicity as a function of depth in the world, and the orientation of stress axes of deep and intermediate earthquakes, are explained using viscous fluid models of subducting slabs, with a barrier in the mantle at 670 km. 670 km is the depth of a seismic discontinuity, and also the depth below which earthquakes do not occur. The barrier in the models can be a viscosity increase of an order of magnitude or more, or a chemical discontinuity where vertical velocity is zero. LongN versus depth, whereN is the number of earthquakes, shows (1) a linear decrease to about 250–300 km depth, (2) a minimum near that depth, and (3) an increase thereafter. Stress magnitude in a subducting slab versus depth, for a wide variety of models, shows the same pattern. Since there is some experimental evidence thatN is proportional toe κσ, where κ is a constant and σ is the stress magnitude, the agreement is encouraging. In addition, the models predict down-dip compression in the slab at depths below 400 km. This has been observed in earlier studies of earthquake stress axes, and we have confirmed it via a survey of events occurring since 1977 which have been analysed by moment tensor inversion. At intermediate depths, the models predict an approximate but not precise state of down-dip tension when the slab is dipping. The observations do not show an unambiguous state of down-dip tension at intermediate depths, but in the majority of regions the state of stress is decidedly closer to down-dip tension than it is to down-dip compression. Chemical discontinuities above 670 km, or phase transitions with an elevation of the boundary in the slab, predict, when incorporated into the models, stress peaks which are not mirrored in the profile of seismicity versus depth. Models with an asthenosphere and mesosphere of appropriate viscosity can not only explain the state of stress observed in double Benioff zones, but also yield stress magnitude profiles consistent with observed seismicity. Models where a nonlinear rheology is used are qualitatively consistent with the linear models.
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  • 150
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    Pure and applied geophysics 128 (1988), S. 683-724 
    ISSN: 1420-9136
    Keywords: Continental crust ; crustal evolution ; isotopes ; mantle ; recycling ; sediment ; subduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In order to understand the evolution of the crust-mantle system, it is important to recognize the role played by the recycling of continental crust. Crustal recycling can be considered as two fundamentally distinct processes: 1) intracrustal recycling and 2) crust-mantle recycling. Intracrustal recycling is the turnover of crustal material by processes taking place wholly within the crust and includes most sedimentary recycling, isotopic resetting (metamorphism), intracrustal melting and assimilation. Crust-mantle recycling is the transfer of crustal material to the mantle with possible subsequent return to the crust. Intracrustal recycling is important in interpreting secular changes in sediment composition through time. It also explains differences found in crustal area-age patterns measured by different isotopic systems and may also play a role in modeling crustal growth curves based on Nd-model ages. Crustal-mantle recycling, for the most part, is a subduction process and may be considered on three levels. The first is recycling with only short periods of time in the mantle (〈10 m.y.). This may be important in explaining the origin of island-arc and related igneous rocks; there is growing agreement that 1–3% recycled sediment is involved in their origin. Components of recycled crustal material, with long-term storage (up to 2.5 b.y.) in the mantle as distinct entities, has been suggested for the origin of ocean island and ultrapotassic volcanics but there is considerably less agreement on this interpretation. A third proposal calls for the return of crustal material to the mantle with efficient remixing in order to swamp the geochemical and isotopic signature of the recycled component by the mantle. This type of recycling is required for steady-state models of crustal evolution where the mass of the continents remains constant over geological time. It is unlikely if crust-mantle recycling has exceeded 0.75 km3/yr over the past 1–2 Ga. Good evidence exists that selective recycling is an important process. Sedimentary rocks preserved in different tectonic settings are apparently recycled at different rates, resulting in a bias in the sediment types preserved in the geologic record. Selective recycling has important implications for the interpretation of Nd model ages of old sedimentary rocks and in the analysis of accreted terranes. Although there is evidence that continental crust was formed prior to 3.8 Ga, the oldest preserved rocks do not exceed this age. It is likely that the intense meteorite bombardment, which affected the earth during the period 4.56–3.8 Ga, coupled with rapid mantle convection, which resulted from greater heat production, caused the destruction and probable recycling into the mantle of any early formed crust. Although crust-mantle recycling is seen as a viable process, it is concluded that crustal growth has exceeded crust-mantle recycling since at least 3.8 Ga. Intracrustal recycling has not been given adequate consideration in models of crustal growth based on isotopic data (particularly Nd model ages). It is concluded that crustal growth curves based on Nd model ages, while vastly superior to those based on K/Ar or Rb/Sr, tend to underestimate the volume of old crust, due to crust-mantle and/or intracrustal recycling.
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    Development genes and evolution 194 (1985), S. 500-500 
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    Development genes and evolution 194 (1985), S. 499-499 
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    Development genes and evolution 194 (1985), S. 498-498 
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  • 154
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    Development genes and evolution 195 (1986), S. 1-9 
    ISSN: 1432-041X
    Keywords: Rana esculenta complex ; Protein synthesis ; Embryogenesis ; Heat shock ; Amphibian oocyte
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    Topics: Biology
    Notes: Summary We have used isotopic labelling and both one-and two-dimensional electrophoretic procedures to analyse the protien synthesis patterns in oocytes and early embryos of three phenotypes of the European green frogs. The results demonstrated that protein patterns of Rana ridibunda and R. esculenta are identical, but that they differ from those of R. lessonae. Progeny of the lethal cross R. esculenta × R. esculenta showed a distinct delay in the appearance of stage-specific proteins during early embryogenesis. The heat-shock response of R. ridibunda and R. esculenta oocytes was found to be identical, but different from that of Xenopus laevis. The implications of these findings, with respect to hybridogenesis in R. esculenta complex and variations in the regulations of heat shock genes in different amphibian species, are discussed.
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    Development genes and evolution 195 (1986), S. 10-14 
    ISSN: 1432-041X
    Keywords: True hermaphroditism ; Domestic fowl ; Testis graft
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    Topics: Biology
    Notes: Summary Two types of hermaphroditism were experimentally induced in genetically female fowls by grafting of embryonic testes in embryos. Of the 27 hermaphrodites observed during the 8 months after hatching, 20 possessed a right testis and a left ovary and 7 a right testis and a left ovotestis. The testes and ovotestes contained seminiferous tubules with a more or less developed germ cell complement, attaining in many cases the early spermatid stage. The interstitial tissue was poorly functional, as shown by the absence of male secondary sex characters. The ovary or ovarian part of the ovotestes possessed numerous small ovarian follicles. The female arrangement of the plumage and the absence of spurs demonstrated the secretion of oestrogens. A mechanism is proposed for explaining this partial masculinization of genetically female gonads, a phenomenon which occurs during the period of embryonic sex differentiation, and is responsible for this experimental true hermaphroditism.
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  • 156
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    Development genes and evolution 195 (1986), S. 33-38 
    ISSN: 1432-041X
    Keywords: Hydractinia ; Hydra ; Homarine ; Nicotinamide ; Pattern formation
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    Topics: Biology
    Notes: Summary Homogenate of coelenterate tissue interferes with metamorphosis in Hydractinia and pattern formation in both Hydractinia, and Hydra. From the extracts two fractions comprising low-molecular-weight compounds with strong metamorphosis-inhibiting activity were separated. One of these contains, as the active compound, homarine (N-methyl picolinic acid). Homarine concentrations down to 10−6 mol/l stop or retard metamorphosis. High concentrations block the continuation of metamorphosis as long as they are maintained in the culture medium and treatment with homarine during metamorphosis influences the proportioning of the future polyp's body pattern. Most of the homarine found in Hydra tissue derives from Artemia given as food. It is not identical with inhibitor I, an activity partially purified from Hydra tissue, which prevents head and foot formation in Hydra.
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  • 157
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    Keywords: Drosophila embryogenesis ; Segmentation ; Genetic mosaics
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    Topics: Biology
    Notes: Summary In order to investigate the localized requirements for the activity of genes that are required in a Drosophila embryo for segmentation, we have analyzed the patterns in genetic mosaics. In this paper, we describe our results with four different X-chromosome linked segmentation loci: armadillo, fused, giant and unpaired. For each locus, we first describe in detail the cuticle phenotype of mutant embryos. We then describe the segmentation patterns in embryos mosaic for these mutations, in each case utilizing the shavenbaby (svb) larval cuticle marker mutation to identify the regions of pattern made by genetically mutant cells. For all four loci, we can identify embryos containing large regions of both mutant and wildtype pattern. In these mosaics the regions of mutant pattern are marked with svb and the genetically wildtype (svb +) cells make wildtype pattern. The interpretations of the patterns in embryos mosaic for fused and unpaired are complicated by the variability of the phenotypes. However, after taking these complications into account, our principal conclusion is that the requirement for embryonic gene activity seems to be primarily cell autonomous. Based on the descriptions of the mutant phenotypes of these four loci and the analysis of the mosaics, we speculate on the possible roles these genes play in the process of segmentation.
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  • 158
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    Development genes and evolution 195 (1986), S. 39-48 
    ISSN: 1432-041X
    Keywords: Brachydanio ; Cortical granule exocytosis ; Ionophore A23187 ; Localized ionophoretic stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of the divalent ionophore A23187 upon unfertilized eggs of the freshwater teleost fish, Brachydanio rerio, have been examined by light, scanning (SEM) and transmission (TEM) electron microscopy. Treatment of eggs with micromolar amounts (1 μM, 10 μM) of A23187 triggers cortical granule exocytosis and elevation of the chorion. However, the exocytosis of cortical granules in ionophore-activated eggs is explosive and occurs more rapidly than in eggs naturally activated in conditioned tap water. Eggs treated with A23187 in a medium lacking extra-cellular calcium also show cortical granule exocytosis, suggesting strongly that egg activation in Brachydanio results from release of calcium primarily from intracellular stores; however, there is a distinct delay in the onset of cortical granule breakdown. Unfertilized eggs exposed to A23187 for 1–5 min show noticeable disturbances in cell surface topography, including loss of microplicae and the appearance of prominent membrane-limited blebs. To determine if cortical granule exocytosis is self-propagating once initiated, A23187 was applied to a localized portion of the unfertilized egg surface, using either a G-50 sephadex gel bead or a 1 mm glass capillary tube. Eggs placed in continuous contact for 15 min with a bead coated with 10 μM A23187 show neither exocytosis of cortical granules nor elevation of the chorion. All eggs exhibit exocytosis when positioned against a glass rod coated with 1 μM A23187. The cortical granule breakdown is partial and restricted to less than 50% of the egg surface in most cells. The complete exocytosis of cortical granules in the zebra danio egg appears to require the stimulation and release of calcium from multiple sites over the cortex.
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    Development genes and evolution 195 (1986), S. 409-416 
    ISSN: 1432-041X
    Keywords: Immunohistochemistry ; Tissue distribution of LDH5 ; Developing chicken embryos
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    Topics: Biology
    Notes: Summary The distribution of A(M) subunits of lactate dehydrogenase (mainly LDH5) in developing muscle, heart, liver, lung, kidney and cartilage tissue of chicken embryos was examined by the indirect fluorescent antibody technique. Antibodies against porcine LDH5, purified by affinity chromatography, were used for this purpose. In special areas of newly formed myofibrils in somitic myoblasts fluorescence was already detected after 4 days of incubation, and located at the same place in muscle tissue of all advanced developmental stages examined. During the myotube stage of muscle development staining was also located in the peripheral thickened cytoplasma of the myotubes. The myocardium did not exhibit any fluorescent staining in the developmental stages examined. Endocardium, epicardium and pericardium, however, were fluorescent in young developmental stages. The liver showed fluorescence in 5- to 8-day embryos mainly in the endothelial cells of the blood sinusoids. In 9- to 12-day embryos the bile ducts became fluorescent. In lungs after 9- to 12-day development the epithelium and the surrounding tissues of bronchi exhibited strong immunofluorescence. The mesonephros exhibited faint granular fluorescence in tubule-forming cells and their membranes after 4–9 days of incubation. Advanced developmental stages only exhibited fluorescent blood cells. This latter staining is at least partly due to non-specific reactions of blood cell membranes with FITC-conjugated anti-rabbit IgG. Cartilage is characterized by non-specific fluorescence, but in embryos older than 8 days strong granular fluorescence of chondrocytes and staining of the perichondrium distinguished sections treated with anti-LDH5 antibodies from control sections reacted only with FITC-conjugated anti-rabbit IgG. In addition, strong fluorescent staining was detectable in certain areas of the 5-day neural tube and faint staining in the mucosa of the intestine from embryos older than 10 days.
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  • 160
    ISSN: 1432-041X
    Keywords: Embryogenesis ; Maternal effect mutant ; Morphogenetic determinant ; Pattern formation ; UV irradiation
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    Topics: Biology
    Notes: Summary The embryonic body pattern of Chironomus samoensis, as well as other chironomids, can be altered dramatically by irradiating their eggs with ultraviolet light (UV). Anterior UV irradiation leads to the formation of double abdomen embryos whose anterior segments are replaced by posterior segments with reversed polarity. Most double abdomens are symmetrical showing a mirror image duplication of the posterior six or seven segments. However, in some cases the anterior end of the double abdomen is shorter, and comprises fewer segments, than its posterior counterpart. These asymmetries range from moderate to extreme. They involve the juxtaposition, at the plane of polarity reversal, of disparate segments. The same range of symmetrical and asymmetrical double abdomens is also formed spontaneously in an apparently mutant strain of C. samoensis. There are striking similarities between this natural variant and the Drosophila melanogaster mutant bicaudal which are also discussed with respect to models of embryonic pattern formation.
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  • 161
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    Keywords: Determination ; Polar lobe ; Unequal cleavage ; Spiralians
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    Notes: Summary The inequality of the first cleavage division of the Chætopterus embryo is caused by the production of a small polar lobe and the internal shifting of the first cleavage spindle. This division produces a two-celled embryo containing a small AB and a large CD blastomere. These blastomeres have different morphogenetic potentials. Only the larvae resulting from isolated CD blastomeres are able to form bioluminescent photocytes, eyes and lateral hooked bristles. The removal of the polar lobe during first cleavage does not have a great effect on development. These lobeless embryos display a normal pattern of cleavages through the time of mesentoblast formation. The resulting larvae are essentially normal, however they do not form functional photocytes. If the CD cell is isolated after the removal of the first polar lobe, the resulting larva is virtually identical to those formed by the intact CD cell except it lacks the photocyte cells. These results indicate that two separate pathways are involved in the segregation of developmental or morphogenetic potential which takes place during first cleavage. One set of factors, which are necessary for photocyte formation, are associated with the first polar lobe. Other factors that are necessary for the formation of the eyes and lateral hooked bristles are segregated by the unequal cleavage which results from an internal shifting of the cleavage spindle. The removal of a large portion of the vegetal region of the embryo during first cleavage leads to the production of larvae which display a decreased ability to form eyes and lateral hooked bristles. These embryos frequently display an abnormal pattern of cleavages. They do not form the primary somatoblast or the mesentoblast. These results indicate that the vegetal region of the CD cell of Chætopterus is analogous to polar lobes which have been studied in other species, and is therefore important in the specification of the D quadrant. These features of the first cleavage of Chætopterus are a combination of those displayed by forms with direct unequal cleavage and other forms which cleave unequally through the production of large polar lobes. The significance of these findings is discussed relative to the origins of these different types of unequal cleavage.
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    Development genes and evolution 196 (1987), S. 30-50 
    ISSN: 1432-041X
    Keywords: Hydrozoan ; Photoprotein ; Calcium channels ; Metamorphosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary There are species of hydrozoans, Eutonina victoria, Mitrocomella polydiademata, and Phialidium gregarium whose eggs contain calcium-specific photoproteins. These cytoplasmic photoproteins are synthesized during oogenesis. During the cleavage stages of embryogenesis they are distributed to all of the cells of the developing planula larva. The amount of photoprotein slowly declines during the development of the planula larva, and markedly declines when the planula undergoes metamorphosis to become a polyp. Oocytes, unfertilized eggs, and fertilized eggs prior to the first cleavage do not produce light when treated with KCl. The ability to respond to KCl appears about the time of first cleavage, and is correlated with the appearance of active membrane responses. Both the KCl response and the action potentials will occur in sodium-free sea water, and both are inhibited by calcium channel blockers. These and other experiments suggest that voltage sensitive calcium channels first become active at about the time of first cleavage. These channels also appear on the same schedule in both unfertilized eggs and in enucleated egg fragments, which have been artificially activated with A23187. Developing planulae produce few or no spontaneous light responses before gastrulation. Later the frequency and magnitude of spontaneous light production increases presumably due to an increasing frequency and magnitude of calcium transients. Both the natural trigger of metamorphosis (bacteria) and an artificial trigger (CsCl) cause a conspicuous series of calcium transients. When these transients are inhibited by calcium channel blockers, metamorphosis is also inhibited.
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  • 163
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    Keywords: Drosophila ; Homoetic ; Mutational analysis ; Transformation ; Distal disc structures
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    Notes: Summary A gene Brista has been identified in chromosome 2R, in the region 60D11-E4, in which mutations cause homoeotic transformation of distal antennal structures to distal leg derivatives, and in which certain alleles also lead to upsets in the formation of distal elements of the legs. This gene is haploinsufficient for the homoeotic phenotype. Several putative null and two hypomorphic alleles have been recovered. The effects of exposure to the non-permissive temperature of a temperature-sensitive allele are cummulative and depend upon the length of the exposure during the period of antennal cell proliferation. It is suggested that this gene contributes to the stability of the state of determination in distal domain of the antennal and leg discs, and its relationship to other genes with similar mutant phenotype is discussed.
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  • 164
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    Keywords: Drosophila ; Dorsalizing mutant ; Phenotypic rescue ; Poly(A)+ RNA ; Cytoplasm
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    Topics: Biology
    Notes: Summary spätzle (spz), a maternal effect gene of Drosophila, is involved in the establishment of the dorso-ventral axis during embryogenesis. Eggs from females lacking the spz gene product develop into completely dorsalized embryos, i.e. the ventral and lateral pattern elements fail to develop. Upon injection of either cytoplasm or poly(A)+ RNA from early wild-type embryos, spz embryos develop lateral pattern elements represented by Filzkörper and in the case of injected cytoplasm additional ventral pattern elements represented by ventral setae. Wild-type cytoplasm retains the rescuing activity longer than the poly(A)+ RNA fraction does, and cytoplasm is always more effective in provoking the rescue than poly(A)+ RNA. Mosaic females containing spz germ cells surrounded by spz + tissues were generated by pole cell transplantations; a mutant genotype in the germ cells is sufficient to produce all aspects of the spz mutant phenotype, suggesting that the maternal source of spz gene product is the germ line.
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    Development genes and evolution 194 (1985), S. 131-139 
    ISSN: 1432-041X
    Keywords: Testes ; Growth ; Y chromosome ; Ribosomal RNA ; Drosophila
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    Topics: Biology
    Notes: Summary A marked growth in the length of testes ofDrosophila hydei males occurred during pupal development. This growth continued over the first 8 days of adult life and in the young adults sperm were not produced until the testes increased approximately threefold in length to about 28 mm. The length of testes is correlated with genetic factors on the X and Y chromosomes. In males lacking a Y chromosome (X/O) or the short arm (YS) of the Y chromosome (X/YL) the testes were about half the length of testes of control males (X/Y) or double Y males (X/Y/Y). Males with deletions of the distal YL chromosome arm had testicular lengths equivalent to the controls. Males with short testes (X/O and X/YL) showed disruptions to spermatogenesis at meiosis and an absence of normal spermatid elongation. Reduction of active ribosomal RNA genes on the X chromosome in X/O caused an increased expression ofbobbed (bb) and a corresponding reduction in length of testes. Severelybobbed X/O males had very few cysts of spermatogonia and these cysts did not develop into primary spermatocytes.
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    Development genes and evolution 194 (1985), S. 148-154 
    ISSN: 1432-041X
    Keywords: Cell image analysis ; Cell kinetics ; Planarian regeneration
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    Topics: Biology
    Notes: Summary The relationships between cell kinetics and nuclear transformations in regeneration were investigated in the planarianPolycelis nigra by means of image analysis. A SAMBA 200 cell image processor was used to compute densitometric, textural and morphological parameters on Feulgen-stained nuclei in the blastema and near the cut 2–96 h after decapitation. On the basis of these parameters, the phase of the cell cycle (G1–G0, S, G2 and M) was identified and the variations in the percentage of cells in the various phases as well as the blastema cell number were computed against time after decapitation. It was demonstrated that the transection is followed by the sequential wasting of the M, G2, S and G1–G0 compartments. The depletion of a compartment was interpreted as being responsible for the subsequent recovery observed in the next one. The results show that cell proliferation at the section level is not sufficient to account for the increase of the blastema cell number during the first 48 h of regeneration, since the doubling time is about 12 h while the average cycle time is 48 h. It is thus suggested that G1–G0 cells migrate toward the section level, at least during the first 2 days of regeneration. Analysis of the nuclear profiles demonstrated that there are two different classes of G1–G0 cells: one corresponding to mature cells with a lot of condensed chromatin distributed in clumps within the nucleus, the other to immature cells with chromatin regularly distributed according to a rather homogeneous pattern. About one G1–G0 cell out of five is immature at the section level before decapitation while four cells out of five are immature as early as 8 h after the cut. This early inversion of the ratio between mature and immature cells argues in favour of an immigration of immature G1–G0 cells to the young blastema, where they are expected to accomplish only one cell cycle, and thus gives rise to mature cells.
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    Development genes and evolution 194 (1985), S. 173-177 
    ISSN: 1432-041X
    Keywords: Mouse ; Morula ; Binucleate cell ; Polyploidization ; Trophoblast
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    Topics: Biology
    Notes: Summary Mouse morulae from two strains were examined in whole mounts after dissociation of embryos into single cells and were analysed in serial sections by light and electron microscopy. One or two binucleate cells per embryo were discovered in a statistically significant number of morulae. The frequency of morulae with binucleate cell(s) was higher in older morulae than in younger ones. Binucleate cells were always the outer cells of the embryo. Their ultrastructure did not differ from the ultrastructure of mononucleate cells. It is suggested that cell binuclearity at the morula stage is a possible way to polyploidization of nuclei, resulting in the formation of primary trophoblast giant cells.
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    Development genes and evolution 194 (1985), S. 178-180 
    ISSN: 1432-041X
    Keywords: Mouse aggregation chimaeras ; Trisomy 12
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    Topics: Biology
    Notes: Summary Mouse aggregation chimaeras consisting of trisomy 12 and normal euploid cells were produced. The analysis of one trisomy 12↔euploid chimaera, using biochemical and cytological markers, showed that the trisomic cells were able to participate in the formation of most tissues including the ovary. On the other hand, no trisomy 12 cells were found in lymphocyte populations, which is most likely due to early selection in this particular cell lineage. The viability of two adult trisomy 12 chimaeras demonstrates that trisomy 12 cells are able to develop beyond the fetal stage which is not observed in completely trisomic fetuses. Furthermore, these chimaeras did not show any sign of a trisomy 12 syndrome, indicating that the trisomy 12 cells were functionally integrated and participated normally in the differentiation of the various tissues. Our results suggest that trisomy 12 in the mouse is not autonomously cell lethal but can be rescued and is perfectly viable in the presence of normal diploid cells.
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    Development genes and evolution 194 (1985), S. 247-256 
    ISSN: 1432-041X
    Keywords: Hydra carnea ; Spermatogenesis ; Cell cycle kinetics ; Stem cells ; Sexual differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Spermatogenesis inHydra carnea was investigated. The cell proliferation and differentiation kinetics of intermediates in the spermatogenesis pathway were determined, using quantitative determinations of cell abundance, pulse and continuous labelling with3H-thymidine and nuclear DNA measurements. Testes develop in the ectoderm of male hydra as a result of interstitial cell proliferation. Gonial stem cells and proliferating spermatogonia have cell cycles of 28 h and 22 h, respectively. Stem cells undergo four, five or six cell divisions prior to meiosis which includes a premeiotic S+G2 phase of 20 h followed by a long meiotic prophase (22 h). Spermatid differentiation requires 12–29 h. When they first appear, testes contain only proliferating spermatogonia; meiotic and postmeiotic cells appear after 2 and 3 days, respectively and release of mature sperm begins after 4 days. Mature testes produce about 27,000 sperm per day over a period of 4–6 days: about 220 gonial stem cells per testis are required to support this level of sperm differentiation. Further results indicate that somatic (e.g. nematocyte) differentiation does not occur in testes although it continues normally in ectodermal tissue outside testes. Our results support the hypothesis that spermatogenesis is controlled locally in regions of the ectoderm where testes develop.
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    Development genes and evolution 194 (1985), S. 271-274 
    ISSN: 1432-041X
    Keywords: Gastrulation ; Ca2+ ionophores ; Morphogenesis
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    Topics: Biology
    Notes: Summary To test whether ionophore-induced changes in Ca2+ flux can effect the rate of morphogenetic movement we compared the normal schedule of gastrulation for yolk plug ingress and anal pore formation with that observed after ionophore treatment. We chose to study this period of gastrulation because any change in the rate of morphogenetic movement can be readily seen as a measure of yolk plug invagination. Gastrulation ends in urodeles as the yolk plug invaginates (stages 11 1/2 to 12) and disappears from the embryonic surface. The anal pore forms concurrently by the constriction of cells encircling the blastopore (stage 12 1/2). It takes 9 h for the stage 12 yolk plug to invaginate completely at 20°C in the salamanderAmbystoma maculatum. The calcium ionophores, A23187 (25μg/ml) or X537A (250μg/ml), induce the yolk plug to invaginate within 2 to 10 min but do not induce the formation of the anal pore by the constriction of the encircling blastoporal cells. This response indicates that ionophoric release of Ca2+ induces the Ca2+-dependent microfilament contraction required for stage 12 yolk plug invagination, but at the same time does not induce the contraction required for anal pore formation.
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    Development genes and evolution 194 (1985), S. 296-300 
    ISSN: 1432-041X
    Keywords: Gap junctions ; Oogenesis ; Freeze fracture electron microscopy ; Insecta
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    Topics: Biology
    Notes: Summary Oocyte-follicle cell gap junctions inTribolium occur in all oogenetic stages studied. During early previtellogenesis the junctions are found exclusively between lateral membranes of oocyte microvilli and the membrane of prefollicle cells. In late previtellogenesis and vitellogenesis the junctions are located between the tips of oocyte microvilli and the flat membranes of the follicle cells. During previtellogenesis gap junctions are infrequent, whereas in the phase of yolk accumulation their number increases considerably, exceeding 17 junctions/μm2 of the follicle cell membrane. It could be shown by microinjection of a fluorescent dye that gap junctions are in a functional state during vitellogenesis. Possible roles of heterologous gap junctions in oogenesis are discussed.
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  • 172
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    Development genes and evolution 198 (1989), S. 65-77 
    ISSN: 1432-041X
    Keywords: Cell communication ; Pattern formation ; Cell differentiation ; trans-regulatory genes ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects ofpolyhomeotic (ph) mutants in imaginal cells have been studied in a clonal analysis. Clones of cells, homozygous forph, sort-out after a few divisions, probably as a consequence of modified cell affinities. The dorso-ventral margin of the wing has special characteristics that retard this phenomenon. The formation and exclusion of a clone of 8–16 cells affect the polarity of the wild-type neighbour cells and can provoke pattern triplications. The results suggest that a defect in intercellular communication prevents the wild-type cells from maintaining coordinated positional information. The cells react by regenerative growth, and reorganize into a new pattern. The pleiotropic phenotypes ofph mutants are explained according to a common hypothesis aboutph + function.
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  • 173
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    Keywords: Hemoglobin transition ; Erythrocytes ; Indirect immunofluorescence ; Metamorphosis ; Xenopus laevis
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    Topics: Biology
    Notes: Summary To elucidate the cellular basis of hemoglobin transition inXenopus laevis the distribution of larval and adult hemoglobins was analyzed by indirect immunofluorescence in the circulating erythrocytes during metamorphosis. In addition, the morphological characteristics as well as the capacity for synthesis of DNA and hemoglobin in the erythrocytes were followed during the same developmental period. Our quantitative analysis on the distribution of larval and adult hemoglobins suggests that they are localized in different cells. Hemoglobin transition, therefore, most likely reflects replacement of the larval erythrocyte population by new cells which are committed to adult globin synthesis. Since hemoglobin transition is not accompanied by an increase in the abundance of immature erythroid cells with active DNA synthesis, we assume that the presumptive adult erythroid cells are released into circulation at a relatively advanced stage of maturation. The decline in the synthesis of DNA and larval hemoglobin further indicates that cessation of cell renewal in the larval erythrocyte population may represent a decisive step in hemoglobin transition.
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  • 174
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    Keywords: DNA injection ; CAT gene expression ; Midblastula transition ; Xenopus embryogenesis ; Coenocytic egg cells
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    Topics: Biology
    Notes: Summary InXenopus laevis embryogenesis, the synthesis of heterogeneous mRNA-like RNA starts at the cleavage stage, whereas that of low-molecular-mass RNAs and rRNA occurs at the early blastula and late blastula stages, respectively. In coenocytic fertilizedXenopus egg cells, which fail to cleave, an excess of exogenously injected DNA (pBR322) induces ‘premature’ expression of previously injected exogenous genes (yeast tRNA genes). We have carried out experiments to discover whether the injection of excess exogenous DNAs of various origins modifies the expression of endogenous genes and previously injected exogenous genes inXenopus embryogenesis. We found that injection of a relatively large amount of exogenous DNA (Xenopus rDNA clone) induces the premature expression, or enhanced expression, of previously injected bacterial chloramphenicol acetyltransferase genes in coenocytic cells. In embryos, however, the injection of exogenous DNAs of various origins did not appreciably modify the expression of either endogenous or previously injected CAT genes. The DNAs injected into fertilized eggs were not degraded and were partitioned into the nuclei of most (at least 80%) of the descendant blastomeres at least during early stages of development. Therefore, we concluded that the program of gene expression in normally developing embryos cannot easily be altered by the introduction of excess exogenous DNAs.
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  • 175
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    Development genes and evolution 198 (1989), S. 85-91 
    ISSN: 1432-041X
    Keywords: Elongated microvilli ; Extracellular matrix ; Elastic support ; Hatching process ; Sea urchin embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The early sea urchin embryo is supported in a concentric position within the perivitelline space by elongated microvilli which are attached to the fertilization envelope by extracellular matrix fibers. This “attachment complex,” of microvillus tip: extracellular matrix fibers: fertilization envelope, was revealed by two methods: the use of pronase or calcium-free sea water to dissolve the extracellular matrix fibers, thus causing the eggs to lose their concentric location, and the visualization of the “attachment complex” using video-enhanced differential interference contrast microscopy and transmission electron microscope images. The presence of the “attachment complex” helps in understanding two types of early developmental events: (1) the apparently continual change in microvillus length during cleavage stages which retains the embryos in their concentric position and (2) the hatching process.
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  • 176
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    Development genes and evolution 196 (1987), S. 273-278 
    ISSN: 1432-041X
    Keywords: Mouse embryos ; Lipid analogues ; Stage-specific antigens ; Lectin binding ; Cell polarization
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    Topics: Biology
    Notes: Summary In two-cell mouse embryos, membrane components detected by a variety of antisera, lectins and lipid analogues and covalent labeling were found to be localized in the poles of the two blastomeres opposite the cleavage furrow. The proportion of polarized blastomeres increased rapidly during the first 4–5 h following first cleavage and then diminished approximately two-fold over the remaining period before second cleavage. Concurrent with this decrease in percent polarization, observed poles were found to be less spatially restricted. This polarization is not the result of a ligand induced capping or a manifestation of differences in surface topography. In light of recent measurements of lateral diffusion, the polarization of membrane components may be significant for the formation of morphogenetic gradients during cleavage.
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  • 177
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    Development genes and evolution 196 (1987), S. 279-285 
    ISSN: 1432-041X
    Keywords: Drosophila melanogaster ; Mutant oogenesis ; Time-lapse filming ; Maternal effect ; Pattern formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Drosophila females homozygous for the mutation dicephalic occasionally produce ovarian follicles with a nurse-cell cluster on each oocyte pole (dic follicles). Most dic follicles contain 15 nurse cells as in the normal follicle, but the total nurse-cell volume is larger in dic follicles; this is in keeping with the increase in DNA content recently described. However, the relative increase in oocyte volume during nurse-cell regression (from stage 10B onward) is not significantly larger in dic than in normal follicles. Time-lapse recordings in vitro show that, as a rule, both nurse cell clusters in a dic follicle export cytoplasm to the oocyte but nurse-cell regression remains incomplete at both poles and the persisting remnants of the nurse cells cause anomalies in chorion shape. The kinematics of cytoplasmic transfer are less aberrant at that oocyte pole which harbours the germinal vesicle. Possible links are discussed between these anomalies of oogenesis and the double-anterior embryonic patterns observed in the majority of developing dic eggs.
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  • 178
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    Development genes and evolution 198 (1989), S. 103-113 
    ISSN: 1432-041X
    Keywords: Morphogenesis ; Keratan sulfate ; Glycosaminoglycan ; Craniofacial morphogenesis ; Chondrogenesis ; Placodes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using the monoclonal antibody MZ15 in immunocytochemical and ultrastructural studies we have been able to determine the spatiotemporal pattern of keratan sulfate (KS) distribution during quail craniofacial morphogenesis. KS-containing proteoglycans are found associated with invaginating placodes (olfactory, lens and otic), in developing pronephric tubules, notochord, pharynx and endocardium, and display developmental regulation. The appearance of such proteoglycans (PGs) during placode morphogenesis is particularly striking and we suggest that they may be an important component of the extracellular matrix which has been previously implicated in mediating the morphogenetic interactions and cell movements occurring at these sites. The otic vesicle during stage 18–22 displays a notable asymmetric distribution of KS-containing PGs. The role that these molecules may play and the reasons for this regionalization are, as yet, unclear but it is conceivable that the distribution of proteoglycans at this stage reflects subsequent differentiative events during otocyst development. Furthermore, our ultrastructural observations indicate that over the developmental period studied (H & H stages 8–22) keratan sulfate exists in at least two proteoglycan forms. Some spatiotemporal correlation has been found to exist between the distributions of KS-containing PGs and type II collagen as previously reported by Thorogood et al. (1986). We suggest that the proteoglycan detected at such sites is cartilage-specific proteoglycan and that it plays an important role, together with type II collagen, in the “signalling” mechanism which specifies the subsequent pattern of the chondrocranium. It is proposed that this interaction at epithelio-mesenchymal interfaces in the developing head parallels the matrix-mediated tissue interaction between notochord and somites which results in the formation of the cartilaginous primordia of the vertebrae from the sclerotomes as reported by Lash and Vasan (1978).
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  • 179
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    Keywords: Egg cortex ; Molluscan egg ; Polarity ; Polar lobe ; Cytoskeleton
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    Notes: Summary We have studied the organization of the cortex in fertilized eggs ofNassarius reticulatus by examining rotary-shadowed whole mounts of isolated cortices in the transmission electron microscope. The following components were distinguished: (a) the plasma membrane, with clathrin-coated areas and coated pits, (b) microfilaments and microtubules, and (c) a tubulovesicular network of endoplasmic reticulum. Microfilaments were identified by labeling with heavy meromyosin, and microtubules with a monoclonal anti-tubulin antibody, using both immunofluorescence microscopy and immunogold labeling for transmission electron microscopy. The microfilaments are organized in a network parallel to and closely associated with the plasma membrane, with typical Y- and X-shaped intersections. The endoplasmic reticulum is associated with this microfilamentous lattice. The microtubules also run parallel to the plasma membrane, but they are located at a greater distance, as can be inferred from stereo images. In the uncleaved egg, numerous microtubules are present in the egg cortex. Shortly before polar lobe formation, at the onset of mitosis, the microtubules disappear almost entirely. They reappear again at the end of first cleavage, as the polar lobe is being resorbed. The synthesis of cortical microtubules at this stage appears to depend on the presence of microtubule-organizing centers in the animal hemisphere of the egg, since microtubules do not reappear in isolated polar lobes. Clathrin-coated areas are present in both the animal and vegetal hemisphere before polar lobe formation. During mitosis, the clathrin-coated plaques and pits are found almost exclusively in the animal hemisphere. After resorption of the polar lobe, at the two-cell stage, no clathrin-coated areas were found at all.
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    Development genes and evolution 198 (1989), S. 129-136 
    ISSN: 1432-041X
    Keywords: Bioluminescence ; Polar lobe ; Spiralian development
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    Topics: Biology
    Notes: Summary The light emitting photocytes ofChaetopterus variopedatus larvae are bilaterally situated within the ectoderm of the post-trochal region. Their histological appearance is similar to that of the adult photocytes. The larval photocytes contain a large quantity of membranous secretory vesicles (photosomes), which probably contain the photoluminescent protein. The two-cellChaetopterus embryo contains a small AB and a large CD blastomere. Previous studies have shown that only the “larvae” resulting from isolated CD blastomeres are able to luminesce. Consistent with these findings, morphologically distinct photocytes are only found in the CD larvae. The removal of the small polar lobe that forms during first cleavage leads to the production of a “larva” that is unable to produce light. All delobed larvae contain morphologically distinct photocytes, which are identical to those in normal larvae except they appear to contain only a small quantity of photosomes. Experimental equalization of first cleavage leads to the production of a double embryo. While photocytes are found in both of the duplicated post-trochal regions, usually only one of these is capable of emitting luminescence. Apparently, the highly localized vagetal material (determinants) responsible for functional light emission is distributed to both halves in only a few cases when first cleavage is experimentally “equalized”. These results indicate that the determinative action of the polar lobe is not required for the formation of the photocytes themselves, but rather for their ability to function as emitters of light. The determinants in the polar lobe ofChaetopterus may control some aspect of the photoluminescence reaction itself, such as the production of the photoprotein.
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  • 181
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    Development genes and evolution 198 (1989), S. 137-140 
    ISSN: 1432-041X
    Keywords: Drosophila embryo ; Spalt gene ; Homeotic gene expression ; Polycomb group
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    Topics: Biology
    Notes: Summary spalt is a gene required for head and tail development in theDrosophila embryo. When mutated it produces homeotic transformations which are restricted in the embryonic cuticle to both ends of the embryo. However analysis of the distribution of its transcripts has shown that it is also expressed in the trunk region of the embruo. Here I show that two homeotic genesUltrabithorax (Ubx) andSex combs reduced (Scr) can be ectopically expressed in the trunk region of embryos lackingspalt gene function. These results suggest thatspalt has a general role in ensuring the correct spatial expression of other homeotic genes and hence could be another member of thePolycomb class of regulatory genes.
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  • 182
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    Development genes and evolution 196 (1987), S. 316-320 
    ISSN: 1432-041X
    Keywords: Cytoskeleton ; Oocyte ; Grey crescent ; Microtubules
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    Topics: Biology
    Notes: Summary Maturing axolotl oocytes which are treated with protein synthesis inhibitors or which are heat-shocked can be induced to reorganize their cytoplasm and to form an early grey crescent. The maturing axolotl oocyte has been used as a model system to study the role of the cytoskeleton in dorsoventral polarization as visualized by grey crescent formation. Results presented here provide evidence for the involvement of microtubules in the formation of the early grey crescent. Whereas inhibitors of microtubule polymerization and antibodies against tubulin both elicit early grey crescent formation, the effect of taxol shows that microtubule polymerization is required at a late stage in this event. The nucleus furnishes important factors required for early grey crescent formation and might play a role in microtubule polymerization.
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    Development genes and evolution 196 (1987), S. 321-327 
    ISSN: 1432-041X
    Keywords: Muscle ontogenesis ; Embryonic chick/mouse chimaeras ; Somite exchange ; Premuscular mass transplants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Heteroclass chick/mouse chimaeras were prepared by transplanting somitic presumptive myogenic cells or limb bud myoblasts from donor mouse embryos into chick hosts, to replace (1) previously extirpated brachial somitic mesoderm or (2) experimentally deleted limb premuscular masses. Since mouse and chick cells can be distinguished by differential staining affinities, this parameter was used to verify the viability of the implant and to assess its fate. Our analyses showed that transplanted mouse somitic myogenic stem cells or limb bud myoblasts did not participate in the host brachial musculature, whatever the experimental conditions.
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  • 184
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    Keywords: Laminin ; Synthesis ; Amphibian ; Oogenesis ; Early development
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    Notes: Summary In the amphibian Pleurodeles waltlii, lamininrelated polypeptides (amphibian-LN) are present in the extracellular matrix underlying the blastocoel roof of gastrulating embryos. Immunoprecipitation with affinity-purified anti-laminin antibodies demonstrated that amphibian-LN is synthesized in oocytes (from stage III onward), eggs and throughout early development. At the late blastula stage, when experiments were carried out with animal and vegetal halves, there were no regional differences in the pattern of amphibian-LN synthesis. The results obtained with transcription inhibitors suggest that throughout pregastrula stages, amphibian-LN is a translation product of stored maternal mRNA. Finally, having compared amphibian-LN and fibronectin synthesis, it is concluded that both extracellular glycoproteins have a common pattern of synthesis.
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    Development genes and evolution 196 (1987), S. 333-337 
    ISSN: 1432-041X
    Keywords: Primordial germ cells ; Dopamine ; Cell shape ; Cell adhesiveness ; Chick embryo
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    Topics: Biology
    Notes: Summary Dopamine (DA), injected beneath the blastodisc of the chick embryo before the beginning of incubation, induced an approximately 2.5 times increase in the total number of primordial germ cells (PGCs) at the definitive primitive streak stage. It also produced changes in the shape and behaviour of PGCs since more than half of them adhered to one another, forming groups or chains of three or more cells and, in contrast to their characteristic spherical form, most single PGCs displayed a fibroblastic appearance. On the 2nd day of incubation most PGCs remained adherent to each other, which did not hinder them from entering the extra-embryonic blood vessels of the crescent. Ultrastructural analysis showed that PGCs adhered to each other by large areas of cell membrane apposition and specialized adhesive structures, such as tight junctions and desmosomes. PGCs also displayed many lamellipodial and filopodial processes. The effects of DA on PGCs were prevented by either glucose or EDTA. Although it is difficult to account for the effect of glucose, the effect of EDTA suggests that the action of DA may be calcium-dependent.
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  • 186
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    Keywords: Juvenile hormone ; Juvenile hormone carrier protein ; Locust embryogenesis ; Serosa
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    Topics: Biology
    Notes: Summary At the end of blastokinesis, serosal epitheliae of 4- to 5-day-old embryos of Locusta migratoria contain an immunohistologically detectable cytosolic protein (Mr ∼240 kDa) which is related to the juvenile hormone carrier-protein in the haemolymph of the same species and which binds tritiated juvenile hormone 3 (JH3) (Kd∼10−8 M). At this early stage of development the corpora allata of the embryo are not yet fully differentiated and do not synthesize JH3 in organ cultures. The earliest detectable JH3 production by corpora allata in isolated heads is on day 6. On the other hand, serosal epitheliae of 4- to 5-day-old embryos produce JH3 in organ cultures, as has been shown by methylation of (10-3H)-JH3-acid to (10-3H)-JH3, and by incorporation of tritiated CH3 from l-(methyl-3H)-methionine into JH3. Isolated heads and abdomens of the embryos used as donors for the serosal preparations did not show methyl transferase activity responsible for JH3 biosynthesis. The serosal cells represent a hitherto unrecognized source of methyl transferase activity and of JH3 production. Degradation of JH3 to JH3-acid was also observed.
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    Development genes and evolution 198 (1989), S. 179-184 
    ISSN: 1432-041X
    Keywords: Egg membrane ; Polarity ; Blastomere ; Sea urchin ; Immuno-electron microscopy
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    Topics: Biology
    Notes: Summary The blastomeres of sea urchin embryos have two surface regions with different properties. Numerous microvilli are present in the apical surface region, while the baso-lateral surface region, either on adjoining adjacent cells or facing the blastocoel, is smooth. When blastomeres are isolated from embryos and stained with fluorescein-isothiocyanate-labelled anti-(egg surface) antibody (anti-ES) prepared against membranes isolated from fertilized eggs, the apical microvillous region fluoresces while the smooth region does not [Yazaki I (1984) Acta Embryol Morphol Exp 5∶3–22]. In order to study quantitatively the ‘bindability’ of the membrane in the two regions to anti-ES, immunoelectron microscopy was used. Blastomeres isolated from embryos ofHemicentrotus pulcherrimus at the eight-cell stage were treated with rabbit anti-ES serum or pre-immune serum and then with ferritin-conjugated goat anti-(rabbit IgG) for 10 min at 0°C, mainly before fixation. About 10 times (maximally 45 times) more ferritin particles were counted per contour length in the microvillous surface region than in the smooth surface region. These results suggest that the membrane of the blastomeres of sea urchin embryos is a mosaic of two different membrane territories: one represented by the microvillous surface originating from the unfertilized egg, which binds anti-ES, the other by the smooth surface newly organized after the first cleavage, which does not react with anti-ES. The mechanism of segregation of the membrane into these two regions is discussed.
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    Development genes and evolution 196 (1987), S. 367-371 
    ISSN: 1432-041X
    Keywords: Vitellogenesis ; Bufo marinus oocyte ; Yolk-platelet membrane ; Ultrastructure
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    Topics: Biology
    Notes: Summary Oocytes of the toad Bufo marinus have been studied by means of thin section and particularly freeze-fracture electron microscopy to characterize the cytoplasmic membranes around the yolk organelle, and the storage of yolk material in precursors and platelets. This appears to be a previously unknown type of yolk-platelet formation. During yolk-organelle development from the primordial precursor to the bi-partite fully grown yolk platelet, numerous lipoid droplets are attached to the periphery of the platelet, indicating an intense uptake of lipids. As is typical for amphibians, the fully grown yolk platelet has a crystalline internum covered by a dense osmiophilic externum, and the whole organelle is enveloped by a plasma membrane that shows no direct connection or fusion with endocytotic vesicles. The yolk membrane exhibits few intramembraneous particles (IMPs) at the core areas and some more where it borders fields of lipoid droplets. Here the IMPs show a net-like arrangement in the furrows between adjacent droplets.
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    Development genes and evolution 196 (1987), S. 356-366 
    ISSN: 1432-041X
    Keywords: Dipteran insect ; Embryogenesis ; Cytoskeleton ; Colchicine ; Cytochalasin B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryos of the paedogenetically reproducing gall midge Heteropeza pygmaea develop floating in the haemocoel of a so-called mother larva. The egg membranes remain permeable and the embryos increase in size during embryonic development by taking up nutrients from the haemolymph. Such embryos can be cultured in vitro, i.e. in haemolymph drops obtained from mother larvae. We tested the effects of several drugs known to interact with cytoskeletal elements on different stages of embryonic development, including cleavage and gastrulation. The drugs were added to the in vitro cultures and the effects were studied with time-lapse cine-micrography. Colchicine and vinblastine blocked cleaving eggs in metaphase stage and arrested yolk globule oscillation. In spite of such a block blastoderms once formed continued development through germ band formation and extension and also increased in size. Cytochalasin B did not affect the stage of cleavage; however, it inhibited gastrulation and subsequent morphogenetic processes and also prevented size increase. We conclude that (1) the functioning of microtubules is needed for yolk globule oscillation during cleavage interphases but not for the gastrulation processes subsequent to blastoderm formation and (2) microfilaments do not play an important role in cleavage, at least not for the orderly succession of the cleavage divisions, but are essential for the morphogenetic movements associated with gastrulation. We suggest that during cleavage a limited stock of microtubules and their precursors is responsible for both transport of chromosomes during mitoses and translocation of organelles during interphase. Yolk oscillation seems to be a secondary effect and of minor or no importance for the normal course of embryonic development.
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    Development genes and evolution 196 (1987), S. 372-375 
    ISSN: 1432-041X
    Keywords: Pattern formation ; Morphogenesis ; Drosophila ; Growth ; Imaginal disc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison of the morphogenetic maps of the notum anlage of Drosophila melanogaster derived from the gynandromorph data and mosaics induced by somatic crossing-over during the first instar larval stage revealed that practically no major morphogenetic movements occur in the development of the anlage between the blastoderm and first instar larval stages and the adult stage. By comparing the morphogenetic map derived from gynandromorphs and the fate map derived from data on the transplantation of fragments of the mature wing imaginal disc, it was observed that no major morphogenetic movements occur in the notum anlage between the stages of the allocation of the disc and the mature disc. The results are consistent with the observations of other authors concerning the larval development of eye-antenna, wing and leg discs.
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  • 191
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    Keywords: Histones ; Sea urchins ; Regulation of gene expression ; Maternal messenger RNA
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    Topics: Biology
    Notes: Summary The ability to specifically delete the store of maternal α-subtype histone mRNAs stored in the egg pronucleus has allowed us to examine the role of this major fraction of the maternal mRNA in the early development of the sea urchinStrongylocentrotus purpuratus. The egg nucleus was removed by centrifugation, and the resulting enucleate half eggs were fertilized. These haploid andromerogones lacked any stored α-subtype histone mRNAs. However, when grown in parallel with control embryos, they showed identical cleavage cycles, cell numbers, and patterns of cell differentiation. Measurements of the amount of α-histone mRNA in these andromerogones showed that there was no premature synthesis of α-histone mRNAs to compensate for the deleted maternal pool. Instead embryonic synthesis was normal in timing of initiation and duration. the ability of these embryos to develop into highly differentiated larvae without their maternal α-subtype histone mRNA pool suggests that this pool is not a critical component of early development per se. This suggestion is strengthened by the observation that the primitive sea urchinEucidaris tribuloides naturally lacks this maternal histone mRNA store. Evolutionary implications are discussed.
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    Development genes and evolution 195 (1986), S. 259-264 
    ISSN: 1432-041X
    Keywords: Pattern formation ; Cell differentiation ; Gene organization ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We studied the genetic bases of threeHairy-wing (Hw 1,Hw Ua ,Hw 49c ) mutations mapping in the region of theachaete-scute complex (AS-C). Analysis of X-ray-induced revertants ofHw 1 andHw 49c uncoveredachaete andscute mutant phenotypes respectively. This indicates that theHw mutant phenotypes result from an excess of function of these genes of theachaete-scute complex (AS-C). The phenotypes of the differentHws show allelic specificity in the pattern of extrachaetes. In addition to these mutations, certain inversions and internal duplications of the AS-C also produce aHw-variegated phenotype, probably due to variegation or decompensation of the genes of the AS-C. The expressivity of the differentHws (mutation or variegation) is modulated by the number of doses of the AS-C present in the genome. A similar dose-dependent modulation is exerted by the transregulatory geneshairy andextramacrochaetae. We discuss these results on the basis of a regulation model of the expression of the AS-C.
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  • 193
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    Keywords: Extracellular matrix ; Mollusca ; Mesoderm determination ; Lectins ; Cell contacts
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    Notes: Summary In 32-cell stage embryos ofPatella vulgata one of the macromeres contacts the animal micromeres, and as a result is induced to differentiate into the stem cell of the mesodermal cell line. In this study we show the presence of an extracellular matrix (ECM) between these two interacting cell types. The ECM appears to be formed by the micromeres during the 32-cell stage. Staining experiments with alcian blue and tannic acid indicate that in contains glycoconjugates, possibly in the form of proteoglycans. The characteristics of the ECM were examined further by fluorescein isothiocyanate (FITC)-lectin labelling. Of 17 lectins tested, concanavalin A (ConA), succinyl-ConA, LCH-B (Lens culinaris) and PEA (Pisum sativum) showed a positive labelling of the ECM. These results are in accordance with the electron microscopic data. The appearance of the ECM at this specific stage and place suggests that it might play an important role in the induction of the mesodermal cell line.
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    Development genes and evolution 195 (1986), S. 281-289 
    ISSN: 1432-041X
    Keywords: Sensory neurons ; Peripheral nervous system ; Pathway formation ; Drosophila embryo ; Neurogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The thoracic and abdominal segments of the Drosophila embryo contain 373 neurons innervating external sensory structures and 162 neurons innervating chordotonal organs. These neurons are arranged in ventral, lateral and dorsal clusters within each segment, in a highly invariant pattern. Two fascicles are formed in each segment as the sensory axons grow ventrally towards the CNS and meet motor axons growing dorsally from the CNS. In all but the last segment, the anterior fascicle is contributed by the dorsal and lateral neurons, while the posterior one is formed by the ventral neurons. Five distinct segmental patterns are described, corresponding to (1) the prothorax, (2) the other two thoracic segments, (3) the first seven abdominal segments, (4) the eighth and (5) the ninth (and possibly the tenth) abdominal segments.
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  • 195
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    Development genes and evolution 196 (1987), S. 414-420 
    ISSN: 1432-041X
    Keywords: Larval hemolymph proteins ; Juvenile hormone-20-hydroxyecdysone ; Fat body ; Stage-specific gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The role of juvenile hormone (JH) and 20-hydroxyecdysone (20-HE) in the stage-specific expression of the larval hemolymph protein (LHP) genes, Lhp82 and Lhp76, was investigated in the waxmoth, Galleria mellonella, larvae. Northern blot and dot hybridization analyses of total RNA in larvae that were undergoing an extralarval molt, induced either by cold shock or by application of JH to day-0 last instar larvae, showed that the last instar-specific Lhp82 transcripts were not present during this molt cycle. Lhp76 transcripts were, however, present transiently. During the course of an extralarval molt induced by JH in day-3 larvae both the transcripts were present but they were relatively less abundant than in the controls. JH had no effect on the relative abundance of the Lhp transcripts when applied to ligated day-3 or older larvae. By contrast, application of 20-HE either to intact or prothorax-ligated larvae of different developmental stages as well as to fat body in vitro resulted in a rapid decrease in the relative abundance of the Lhp transcripts. The natural decrease in the Lhp transcript levels that occurs in wandering and spinning last instar larvae was blocked in ligated larvae, probably due to deprivation of the source of the endogenous ecdysteroids. These observations suggest that ecdysteroids serve as the natural cue to turn off the Lhp genes at each molt and that JH blocks activation of only the last instar-specific Lhp82 gene.
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  • 196
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    Development genes and evolution 196 (1987), S. 421-433 
    ISSN: 1432-041X
    Keywords: Cell surface patterns ; Mutations affecting patterns ; Ciliate development ; Tetrahymena thermophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A single-gene recessive mutation, bcd (broadened cortical domains), of Tetrahymena thermophila is characterized by a variable broadening of the spatial domains within which cortical organelles, including both the contractile vacuole pores (CVP) and oral apparatus (OA), are formed. The phenotype is not temperature-sensitive. During the development of the organelles of the mutant prior to cell division, extra CVPs and extra oral primordia (OP) appear near ciliary rows adjacent to the rows at which these structures normally form. In the later stages of development, some, but not all, of these extra structures are resorbed, or in the case of the oral domain, multiple adjacent OPs may be completely or partially integrated into a single enlarged OA. When multiple OAs persist, one or more of these may display a reversed orientation reminiscent of those encountered in janus mutants. However, unlike janus, bcd cells do not express any sign of a mirror-image global organization. Our results can best be accounted for by postulating that the bcd mutation affects some common determinant of the widths of both CVP and OA domains. Studies are in progress which explore the relationship between this width-determining mechanism(s) and the mechanism(s) determining the location of cortical organelles around the cell circumference.
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  • 197
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    Development genes and evolution 195 (1986), S. 338-343 
    ISSN: 1432-041X
    Keywords: Drosophila melanogaster ; Imaginai discs ; Aldehyde oxidse ; Determination ; Field size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The pattern of aldehyde oxidase (AO) activity was determined in wing discs of Drosophila melanogaster larvae homozygous for the mutants apt 73n, Beaded, and vestigial (vg) in order to determine if reduction in field size in the pouch could be related to alterations of the wild-type AO pattern, as suggested by the Kauffman (1978) hypothesis. The pattern in wild-type discs was resolved into six areas for comparison with mutant discs. vg discs developed at 25° C showed restriction of the pattern into a small area on the anterior side of the disc, and comparison of vg and wild-type prepupal wings allowed positive identification of the AO pattern elements which remained. AO patterns in vg wing discs grown at 27°, 29°, and 31° C were progressively more complete and similar to wild-type, reflecting the reduction in cell death in discs grown at higher temperatures. These results show that cell loss during the third instar in vg development at 25° C is responsible for the alteration of the AO pattern, rather than field size reduction, and that determination of the pattern must take place much earlier than the time of its first appearance during the third larval instar, and before cell death in vg discs begins. Thus mutants acting at earlier stages will be necessary for further tests of the Kauffman hypothesis.
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  • 198
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    Development genes and evolution 196 (1987), S. 467-471 
    ISSN: 1432-041X
    Keywords: Leydig cells ; Ethane dimethanesulphonate ; Destruction ; Regenerative capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A single intraperitoneal injection of ethane dimethanesulphonate (EDS) destroys all Leydig cells in the adult rat testis but 1–2 weeks later new foetal-type Leydig cells begin to regenerate within the interstitial tissue. A further EDS treatment at 4 weeks failed to kill the new population of foetal-type Leydig cells. Between 10–20 weeks, the new Leydig cells exhibited the characteristics of adult-type Leydig cells. These cells responded to another EDS treatment by exhibiting a second phase of complete degeneration followed by regeneration of a foetal-type and subsequently an adult-type cell population. The results indicate that the testis retains the ability to replenish its supply of Leydig cells despite successive phases of total degradation of Leydig cells.
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  • 199
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    Development genes and evolution 195 (1986), S. 417-432 
    ISSN: 1432-041X
    Keywords: Gene regulation ; Developmental pathways ; Antennapedia and Bithorax complexes in Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have studied the embryonic and adult phenotypes of genetic combinations between Polycomb (Pc), Regulator of bithorax (Rg-bx) and the genes of the Bithorax complex (BX-C) and the Antennapedia complex (ANT-C). The products of Pc and Rg-bx genes act antagonistically, their mutant combinations leading to the ectopic expression of genes of the BX-C and ANT-C. The genetic analysis of the Pc locus suggests it is a complex gene. Pc+ products behave as members of a “regulatory set” that negatively control the expression of BX-C and ANT-C genes. Genetic combinations between different doses of Pc, Rg-bx and the genes of the BX-C and ANT-C have phenotypes which may be interpreted as resulting from ectopic derepression of posterior selector genes repressing selector genes of anterior segments. The transformation phenotypes of certain genetic combinations differ in embryos and adults. A model of regulation of the BX-C and the ANT-C genes during the imaginal cell proliferation is presented, in which the specification state is maintained by self-activation of a given selector gene and down modulation of other selector genes in the same cell.
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  • 200
    ISSN: 1432-041X
    Keywords: Dorsoventral polarity ; Egg organization ; Spiral cleavage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The egg of the polychaete Chætopterus, like many spiralian embryos, undergoes unequal cleavage during the first two cell divisions following fertilization. The first cleavage gives rise to a large CD blastomere and a smaller AB blastomere. At second cleavage the CD blastomere divides asymmetrically, forming a large D blastomere and a smaller C blastomere while the AB cell divides to give rise to the two smaller blastomeres, A and B, which are virtually identical in size. As in many of the other spiralians which have been studied, the A, B, C, and D blastomeres give rise to characteristic portions of the larval body plan. It has been shown in these related forms that the D blastomere derivatives are necessary for the production of various structures derived from the remaining three quadrants, as well as in organizing the overall symmetry properties of the embryo. If Chætopterus embryos are compressed underneath a coverslip at the appropriate stage of development prior to first cleavage (the “pear stage”) some of the embryos divide to form two equal sized blastomeres. Each of the two cells in equalized embryos appears to develop as a normal CD blastomere often dividing to generate a four-celled embryo with two C and two D blastomeres opposite one another (CDCD embryos). These embryos often grow up to form larvae which have duplicated structures. We investigated the role of the early cleavage program in the production of double larvae by staining individual blastomeres of normal and equalized embryos with Nile blue sulfate. Our results reveal that previous descriptions of the symmetry properties of twinned larvae are incorrect. Twins are not true Janus larvae of the duplicitus cruciata form but are organized along a single axis. The two halves face one another along a plane of mirror symmetry, each one fused to the other at their ventral surface just above a single, common, fused mouth. Furthermore, the median plane of the two fused heads is the same as that of the two trunks. These labeling studies indicate that cells derived from both of the two C blastomeres in compressed embyos contribute to the formation of similar structures in both halves of the double larva. The two C quadrants within the double embryos each can give rise to two eyes, and the plane of mirror symmetry between the two halves of the double embryo as mentioned above corresponds to a plane running through the two C blastomeres in the fourcelled CDCD embryo. These findings indicate that the two D quadrants in these double embryos interact to organize the development of the double embryo in a coordinating manner. Both D quadrants, therefore, appear to be inductively active within the CDCD double embryos. These results are discussed in view of various hypotheses which have been proposed to explain the phenomenon of twinning in spiralians.
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