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  • wheat  (144)
  • Springer  (144)
  • 2015-2019
  • 1985-1989  (144)
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  • Springer  (144)
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  • 1
    ISSN: 1420-9071
    Keywords: Hydroxamic acid ; cereals ; maize ; wheat ; aphids ; greenbug
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 2-β-Glucosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA-Glc), the main hydroxamic acid from maize and wheat, and its aglucone, decreased survival ofSchizaphis graminum reared on artificial diets. Both compounds were toxic for aphids and acted as feeding deterrents, at concentrations as low as 1 mM. The natural concentrations of glucosides of hydroxamic acids in wheat leaves reach up to 6 mmoles/kg fresh weight, thus falling within the range in which DIMBOA-Glc causes delecterious effects to diet-fed aphids.
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  • 2
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    European journal of plant pathology 92 (1986), S. 127-132 
    ISSN: 1573-8469
    Keywords: Sitobion avenae ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Teneinde de tijd nodig voor het tellen van graanluizen te beperken kan een minimale monstergrootte bepaald worden bij een van te voren vastgestelde nauwkeurigheid. Om deze monstergrootte uit te kunnen rekenen aan de hand van een voorspelling van de dichtheid moet er een betrouwbare relatie bestaan tussen de gemiddelde dichtheid en de ruimtelijke variantie. Uit analyse van veldgegevens voor de graanluisSitobion avenae blijkt dat een dergelijke relatie bestaat, en dat deze constant blijft tijdens het hele groeiseizoen.
    Notes: Abstract Analysis of field samples of the aphidSitobion avenae on winter wheat revealed that the power-relation between mean density and between-tiller variance did not change during the seasonal growth and decline of the aphid population. A single equation, therefore, could be used throughout the season to calculate the optimal sample size — the number of tillers that must be examined to yield an accurate estimate of aphid density.
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  • 3
    ISSN: 1573-8469
    Keywords: Sitobion avenae ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Om in de praktijk gebruik te kunnen maken van incidentie (de fractie bezette halmen) voor het schatten van graanluispopulaties moet dezelfde relatie tussen dichtheid en incidentie tijdens het hele groeiseizoen gelden. Uit analyses van veldgegevens voor de graanluisSitobion avenae blijkt dat wel het geval te zijn, zodat bepalingen van incidentie kunnen worden gebruikt om de populatiedichtheid betrouwbaar te schatten.
    Notes: Abstract The relation between the population density of the cereal aphid,Sitobion avenae, and the proportion of wheat tillers infested is examined. Three of the six models considered require the assumption that the aphids' spatial configuration can be described by a single statistical distribution; as this is not true forS. avenae these models are unsuitable. When the other three models were applied to field sample data, only that of Nachman (1981) yielded a regression equation that remained constant throughout the development of the aphid population.
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  • 4
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    Theoretical and applied genetics 76 (1988), S. 933-940 
    ISSN: 1432-2242
    Keywords: Gliadin ; Two-dimensional electrophoresis ; F1 monosomic analysis ; chromosomal assignment ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gliadin components from four bread wheat cultivars: Chinese Spring, Capelle Desprez, Holdfast and Pane-247 and their monosomic F1s for the chromosomes of homoeologous groups 1 and 6 have been analyzed by two-dimensional (2-pH) polyacrylamide gel electrophoresis. Chromosomal location of gliadin genes and the allelic differences were well established by analyzing the different F1 monosomic hybrids, electrophoretical patterns and differences in relative staining intensity. A new gliadin encoded by a gene located on chromosome 6B in Chinese Spring is described. The two-dimensional patterns of gliadin in the other three varieties and the chromosomal location of their genes are reported for the first time. Relationships between gliadins in the two-dimensional patterns and the traditional system for their nomenclature are discussed.
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  • 5
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    Nutrient cycling in agroecosystems 15 (1988), S. 123-136 
    ISSN: 1573-0867
    Keywords: corn ; soybean ; wheat ; maximum yield ; optimum yield ; Bray P1 ; exchangeable K
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Data from 32 years of a rotation-fertility experiment were analyzed to determine the average P and K application rates required for maximum yield and for optimum yield. A four-year rotation of corn, soybean, wheat, hay was used for the first 10 years and then changed to corn-1, soybean, wheat, corn-2. Rates of P application per 4-year rotation ranged from 0 to 196 kg ha−1 and for K from 0 to 558 kg ha−1. Multiple regression equations were fitted to the mean yields per 4-year rotation for the response of each crop to P and K applications. The range in P application rates in kg of P per 4-year rotation required to get maximum yields of corn was 118 to 172, for soybeans was 134 to 150, and for wheat was 116 to 138. The range in K application rates in kg K per 4-year rotation to get maximum yields of corn was 378 to 411, for soybeans was 324 and 476, and for wheat was 11 to 323. For rates of application where P and K added exceeded crop removals, soil test P and K increased linearly with the cumulative positive balance of P and K. Where crop removal exceeded application rate, no relation was found between crop removal and soil test.
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  • 6
    ISSN: 1573-0867
    Keywords: farmyard manure ; maize ; nitrogen ; phosphorus ; rice ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments with rice-wheat rotation were conducted during five consecutive years on a coarse-textured low organic matter soil. By amending the soil with 12t FYM ha−1, the yield of wetland rice in the absence of fertilizers was increased by 32 per cent. Application of 80 kg N ha−1 as urea could increase the grain yield of rice equivalent to 120 kg N ha−1 on the unamended soil. Although the soil under test was low in Olsen's P, rice did not respond to the application of phosphorus on both amended and unamended soils. For producing equivalent grain yield, fertilizer requirement of maize grown on soils amended with 6 and 12 t FYM ha−1 could be reduced, respectively to 50 and 25 per cent of the dose recommended for unamended soil (120 kg N + 26.2 kg P + 25 kg K ha−1). Grain yield of wheat grown after rice on soils amended with FYM was significantly higher than that obtained on unamended soil. In contrast, grain yield of wheat which followed maize did not differ significantly on amended or unamended soils.
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  • 7
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    Nutrient cycling in agroecosystems 20 (1989), S. 59-66 
    ISSN: 1573-0867
    Keywords: Ammonia volatilization ; nitrogen leaching ; denitrification ; time of N application ; wheat ; Triticum aestivum ; Triticale ; irrigation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Grain yield, nitrogen (N) assimilation, ammonia (NH3) volatilization, denitrification and fertilizer N distribution were examined in three commercially grown cereal crops; two were sown into conventionally tilled fields, while the third was direct drilled into an untilled field. The crops were top dressed with urea at establishment, tillering or ear initiation. Crop yield and N assimilation were measured in 16 m by 2.5 m plots receiving 0, 35, 70, 105, 140 or 175 kg N ha−1. A mass balance micrometeorological technique was used to measure NH3 volatilization, and other fertilizer N transformations and transfers were studied using15N labelled urea in microplots. On the conventionally tilled sites application of urea increased the grain yield of wheat from 3.9 to 5.5 t ha−1, when averaged over the five application rates, three application times and two sites. There were no site or application time effects. However, on the direct drilled site, time of application had a significant effect on grain yield. When urea was applied at establishment, grain yield was not significantly increased and the mean yield (2.81 t ha−1) was less than that obtained from treatments fertilized at tillering or ear initiation (4.09 and 4.0 t ha−1, respectively). Much of the variation in grain yield at the no-till site could be ascribed to differences in NH3 volatilization. At the no-till site, NH3 losses were equivalent to 24, 12 and 1% of the N applied at establishment, tillering and ear initiation, respectively. Negligible volatilization of NH3 occurred at the other sites. The surface soil at the no-till site had the highest urease activity and the soil was covered with alkaline ash resulting from stubble burning. Plant recovery of fertilizer N did not vary with application time on conventionally tilled sites (mean 62%). However, plant recovery of15N applied to the no-till site at establishment (35% of the applied N) was significantly less than that from plots where the application was delayed (45% at tillering and 55% at ear initiation, respectively). Leaching of N to below 300 mm depth was minimal (0 to 5% of the applied N). The calculated denitrification losses ranged from 1% to 14% of the applied N. The results show that the relative importance of NH3 volatilization, leaching and denitrification varied with site and fertilization time. The importance of the various N loss mechanisms needs to be taken into account when N fertilization strategies are being developed.
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  • 8
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    Nutrient cycling in agroecosystems 19 (1989), S. 137-142 
    ISSN: 1573-0867
    Keywords: S ; calcite ; dolomite ; soybean ; corn ; wheat ; soil acidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Liming costs have escalated since the mid 1970's in the United States. Studies of crop response to lime with irrigation are limited as well as those of crop response to soil acidifying agents. This study was conducted to determine yield response of irrigated soybean [Glycine max (L.) Merrill], corn (Zea mays L.), and wheat (Triticum aestivum L.) to lime and S and the change in soil pH, in response to lime and S. Irrigated soybean, corn, and wheat were grown on Dothan and Tifton loamy fine sand (Plinthic Acrisols) with different levels of calcite, dolomite, and S. Soil samples were collected before applying treatments and during each growing season. Soil pH and Mehlich I extractable P, K, Ca, and Mg in addition to grain yield of each crop were determined. Highest soybean yield (4.2 t ha−1) occurred in 1984 at a soil pH of 4.9 (1:1 v/v soil—water suspension) while the yield was zero at a pH of 3.7 on S treated plots. A soil pH of 4.8 in 1985 reduced soybean yield from 3.4 to 2.7 t ha−1 in comparison to untreated plots (pH = 5.6). In 1986, soybean yield was 0.8 t ha−1 at pH 4.0 in comparison to 2.3 t ha−1 at pH 5.1 and 5.9. Corn did not respond to lime with control pH of 5.3 in 1985 or 5.1 in 1986 but S at pH 4.6 reduced yield from 12.3 to 8.7 t ha−1 in 1985 and S reduced yield from 11.0 to 0.9 t ha−1 at pH 4.0 in 1986. Sulfur reduced wheat yield from 4.3 to 1.7 t ha−1 in 1985 and from 2.2 to 0.9 t ha−1 in 1987. Soil pH after cropping with S addition was 4.4 each year. Wheat did not respond to lime when unlimed soil had a pH of 5.2 or above.
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  • 9
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    Nutrient cycling in agroecosystems 6 (1985), S. 171-176 
    ISSN: 1573-0867
    Keywords: Chloride ; phosphorus ; salinity ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A pot study with four levels each P (control, 6.5, 13.0, 19.5 mg kg−1) and Cl− in irrigation water (control, 30, 60, 90me l−1) was carried out to test Cl− and P interaction on wheat. It has been found that optimum P nutrition alleviates the toxic effect of excess Cl−. Highest P rate resulted in a significant yield increase upto a Cl− level of 60me l−1. Cl− depressed P content in the plant only at a Cl− level of 90me l−1, while P rates had no major impact on the Cl− concentration in the plant. Lower Cl− concentration at higher P rates are rather a dilution effect than an antagonistic one. With optimum P supply higher Cl− contents in the plants were tolerated than with a low P supply.
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  • 10
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    Nutrient cycling in agroecosystems 6 (1985), S. 219-224 
    ISSN: 1573-0867
    Keywords: cowpea ; green gram ; pearl millet ; phosphate ; residual effect ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a four year study on a wheat-green gram (or cowpea) — pearl millet intensive cropping system a total production of 9–10 tonnes of wheat equivalents per year removed 29–30kg P ha−1. If only 26 kg P ha−1 was used then total grain production as well as P uptake, was highest when all the P was applied to wheat. Only when amounts larger than 26 kg P ha−1 were applied was it justified to apply P to pearl millet and green gram (or cowpea). Productivity of the cropping system increased up to 58.5 kg P ha−1 and at this level two thirds of P was applied to wheat, while pearl millet and green gram or cowpea received the remaining one-third. A positive P balance in soil was observed only when 26 k P ha−1 yr−1 or more was applied. Pressure of growing population and per capita diminution or arable land has focussed attention on multiple cropping systems in many Asian countries [1, 2]. In North-Western India the cropping system changed from a single rainy (July–October) or winter (November–April) crop a year prior to the 1960's to two-crops-a-year (both a rainy season and winter crop) in the 1970.s and then in the late 1970's a third summer (May–June) crop was also included. Wheat — green gram (or cowpea) — pearl millet is such a three-crops-a-year multiple cropping system. Phosphate is the costliest major plant nutrient in India and farmers following multiple cropping systems are keen to know the way the phosphate should be apportioned to different crops in a cropping system particularly when small amounts of P are applied. Such information can come only from long-term P fertilization experiments [3, 4]. The objective of the present experiment on a wheat-green gram (or cowpea) — pearl millet multiple cropping system was to study the direct and residual effects of P applied to one crop on the other crops grown in succession and to find the best possible way in which a limited amount of P could be apportioned between the different crops in the rotation. An attempt has also been made to work out the P balance in soil.
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  • 11
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    Nutrient cycling in agroecosystems 8 (1985), S. 91-96 
    ISSN: 1573-0867
    Keywords: chickpea ; Egyptian clover ; field experiments ; K uptake ; mustard ; wheat ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment on a potassium deficient Typic ustochrept soil of the University Farm at Pura, Kanpur compared the responses of wheat, chickpea, mustard and Egyptian clover to added potassium for two consecutive years commencing in 1979–80. All the crops responded to potassium fertilization. Sensitivity of various crops to potassium deficiency was in the order: Egyptian clover 〉 chickpea 〉 wheat 〉 mustard. Higher sensitivity of Egyptian clover and chickpea compared to wheat was in agreement with their comparatively high root cation exchange capacity. Mustard by contrast showed higher root cation exchange capacity and exhibited least potassium sensitivity because of its higher potassium utilization efficiency. Leaf K content and total uptake of potassium by all crops was increased by applying potassium upto the highest level.
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  • 12
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    Nutrient cycling in agroecosystems 8 (1985), S. 97-100 
    ISSN: 1573-0867
    Keywords: critical level ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies were conducted in ten non-calcareous arid brown soils (India) to determine the critical level of soil Zn for predicting response of wheat to zinc fertilization. The per cent mean response at 5 mg kg−1 added Zn varied from 1.3 to 51.4 with a mean value of 17.5 per cent over control in terms of grain yield (g pot−1). Further, Zn application resulted in significant increase in Zn concentration in various plant parts in all the soils irrespective of the initial Zn status. The critical level of Zn in soil and plant below which response to applied Zn may be expected was found to be 1.75 mg kg−1 for 0.1 N HC1 extractable soil Zn and 1.7 mg kg−1 for plant tissue Zn.
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  • 13
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    Nutrient cycling in agroecosystems 8 (1985), S. 173-178 
    ISSN: 1573-0867
    Keywords: critical limit of Mn ; highly permeable soils ; methods and rates of Mn ; Mn deficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two field experiments were conducted on Mn-deficient soils to evaluate the efficiency of rates, methods and time of MnSO4.H2 O application for wheat. Manganese sulphate was broadcast and mixed in soils at the rate of 5 to 50kg Mn ha−1 before seeding and 10 to 40 kg Mn ha−1 as top dress at 28 days — just before first irrigation. Three sprays of 1% MnSO4·H2O unneutralised solutions were applied, the first at 26 days — 2 days before first irrigation and the others afterward at weekly intervals. Both the methods caused a significant and marked increase in grain yield. Three foliar sprays were as effective as soil applications of 20 to 40 Kg Mn ha−1 before seeding. The difference in grain yield resulting from soil applications of Mn before seeding and applications at the first irrigation was not significant. The DTPA-Mn status of 20 fields, selected on the basis of varying degree of Mn deficiency, was related to grain yield (r = 0.77**). Also grain yield of all the experiments had a significant correlation with Mn content of grain (r = 0.55** to 0.82**) and straw (r = 0.77** to 0.82**). The critical limits calculated by statistical method were 1.25, 2.18 and 3.5 mg Mn kg−1 soil for severe deficiency, deficiency and latent deficiency respectively for wheat.
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  • 14
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    Nutrient cycling in agroecosystems 8 (1986), S. 307-311 
    ISSN: 1573-0867
    Keywords: fertilizer P recovery ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Labelled Ca(H2PO4)2 · H2O was added to two soils (an Oxisol and a vertisol) at two rates, both as a point source and completely dispersed through the soil. The point source treatments included two spatial distributions at each of two percentages of soil volume fertilized. Total and fertilizer phosphorus uptake by wheat (Triticum aestivum) grown for 42 days were determined in a glasshouse experiment. Uptake of fertilizer phosphorus was not affected by spatial distribution, but declined in the Oxisol as percentage soil volume fertilized increased at the low application rate. The implications of these results in terms of the effects of cultivation on fertilizer availability are briefly discussed.
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  • 15
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    Nutrient cycling in agroecosystems 9 (1986), S. 223-228 
    ISSN: 1573-0867
    Keywords: crop rotation ; fertilizers ; groundnut ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Investigations were carried out in a long term field experiment from 1976 to 1982, on a loamy sand soil to find out the fertilizer requirements of groundnut and wheat grown in fixed rotation. Application of 26 kgP/ha to wheat alone was found to be sufficient for both wheat and succeeding groundnut. Application of phosphorus to both wheat and groundnut did not result in extra beneficial effect over P application to wheat alone. However, application of 26 kg P/ha to groundnut alone was not sufficient for succeeding wheat. There was no response from K application (25 kg K/ha) in either of these two crops. Increasing the dose of N from 50–150 kg/ha to wheat significantly increased the grain yield of wheat but the pod yield of succeeding groundnut were markedly lowered. Response of wheat to 150 kg N/ha was more marked when P was also applied to wheat and response was less when P was applied to preceding groundnut alone. Zinc application at 6.25 kg/ha to wheat alone resulted in significant increase in grain yield of wheat and pod yield of succeeding groundnut.
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  • 16
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; electrophoresis system ; acid polyacrylamide gel ; gliadins ; variety identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In this paper we describe the method of wheat gliadin electrophoresis in use at RIVRO, Wageningen. It differs from other techniques mainly by the application of an alternative buffer system, making it possible to polymerise the gels in a buffered alkaline environment and to perform the run at pH 3.1 without extensive buffer changing steps. Advantages are a greater gel reproducibility and the ease of gel handling. Furthermore, a rationalised protein extraction procedure, a cheap shaking system for staining baths and a better (slower moving) tracking dye are described.
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  • 17
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    Euphytica 36 (1987), S. 221-224 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; auricle color ; compact head ; stem rust resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Previous evidence suggested that the gene for red auricle (Ra) might be linked to the gene Sr6 for stem rust resistance on chromosome 2D. However, tests indicated that Ra is not linked to either Sr6 or C, which is also on 2D.
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  • 18
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    Euphytica 36 (1987), S. 257-263 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp.tritici ; leaf rust ; partial resistance ; race nursery ; hostpathogen interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten wheat leaf rust (Puccinia recondita f.sp.tritici) race nurseries were planted in 1984. In five, each with a different race, no randomization of the 20 wheat genotypes was carried out. The remaining five race nurseries, also each with one of the same five races, were completely randomized. Comparison of the wheat genotype assesments was done using three procedures: i) Correlating (Pearson's and Spearman's r) the leaf area affected of the twenty wheat genotypes in the randomized and the non-randomized nurseries; ii) comparing the best five wheat genotypes in each race nursery; iii) comparing the Wi-indeces of wheat genotypes in the randomized and non-randomized nurseries. All three procedures showed excellent agreement between the randomized and the non-randomized nurseries . The use of a spreader row was most likely the reason for the large similarity. The consequence is that randomization is not sufficiently relevant to justify the increase in risk of mistakes and the extra administration needed. The host genotype-pathogen race interaction was significant and rather large. All but two genotype-race combinations showed a susceptible infection type, which indicates that partial resistance of wheat to wheat leaf rust caused the major part of this interaction. The results which suggest a gene-for-gene relationship, support the \lsintegrated concept\rs of Parlevliet and Zadoks.
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  • 19
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; crossability ; timing of pollination ; frequency of fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of manipulating the timing of pollination on the fertilization frequency in wheat × tetraploid Hordeum bulbosum hybridizations was investigated as a possible means of overcoming varietal incompatibility in this cross. Pre- and post-anthesis pollinations were made on previously identified highly crossable and poorly crossable wheat genotypes and the stage of development of the individual florets at pollination was expressed in ‘days from anthesis’ units. In both crossable and non-crossable wheat genotypes, a significant negative linear relationship was observed between floret age and fertilization frequency. The magnitude of the response of early pollination was dependent on the number of alleles for non-crossability present at the Kr loci. The non-crossable cultivar Highbury, possessing more than one allele for incompatibility, showed a slight response. The greatest response was shown by the highly crossable cultivar Chinese Spring, possessing alleles for crossability at all Kr loci. The single chromosome substitution line, Chinese Spring (Hope 5B), possessing the most potent allele for non-crossability, Kr 1, showed an intermediate response. The H. bulbosum genotype had no effect on the magnitude of this response, influencing only the mean fertilization frequency.
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  • 20
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; pollen grain germination ; pollen tube growth ; frequency of fertilization ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A quantitative examination of pollen grain germination and pollen tube growth within the overy wall was made in selected wheat × tetraploid H. bulbosum hybridizations, to investigate the effect of the wheat and the H. bulbosum genotype on these characters. As expected, variation at the known crossability loci had no effect on pollen grain germination. The frequency of pollen tube penetration of the ovary wall was, however, severely reduced when the dominant alleles were present. Pollen tube penetration was nevertheless observed in 3 of the 110 ovaries examined of the non-crossable cultivar Highbury. The H. bulbosum genotype had a much smaller effect on these characters, but significant differences between the clones were observed in the numbers of pollen tubes initially penetrating the ovary wall. Although two H. bulbosum genotypes showed no significant differences in the number of ovaries with pollen tubes at the base of the ovule, significant differences in the frequency of fertilization were observed. The possible cause of this discrepancy is discussed. The frequency of fertilization in crossable wheat × H. bulbosum hybridizations was improved by the application of gibberellic acid within 10 minutes of pollination, and reduced by an increase in the ambient temperature from 20°C to 26°C. Fertilization following the pollination of non-crossable wheat genotypes was not affected by either of these factors.
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  • 21
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    Euphytica 36 (1987), S. 521-523 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; plant height ; dwarfing gene ; Rht 12 ; F2 monosomic analysis ; chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal location of the dwarfing gene Rht12 in the mutant winter wheat Karcagi 522M7K was investigated using F2 monosomic analysis. The segregation ratio for F2 progenies of Chinese Spring monosomics × Karcagi 522M7K, and that of Cheyenne monosomics × Karcagi 522M7K indicated that the near complete dominant dwarfing gene Rht12 is located on chromosome 5A. The heterozygous and hemizygous states of the genes Rht12 have the same effect on plant height.
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  • 22
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; frequency of fertilization ; seed set ; seed survival ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An investigation was made of factors affecting the frequency of development of fertilized ovules following wheat x Hordeum bulbosum hybridizations. Seed survival, a term used here to represent the relationship between seed set and fertilization, was shown to vary between the wheat genotypes tested, but was unrelated to the crossability genes present within the wheat genotype. A high seed survival was obtained in crosses involving the variety TH3929 and the single chromosome substitution line, Chinese Spring (Hope 5B). Chinese Spring showed a poor seed survival. Some influence of the H. bulbosum genotype on seed survival was detected. The two environmental factors studied, namely the post-pollination application of gibberellic acid and the ambient temperature, were both shown to affect seed survival. The application of gibberellic acid immediately after pollination or 1 and 2 days after pollination improved seed survival, although the extent of the response was dependent upon the number and timing of the application(s). A genotype-dependent response to the ambient temperature was observed, TH3929 showing a slight but insignificant reduction and Chinese Spring a significant improvement in seed survival with an increase in temperature from 20°C to 26°C. The physiological processes which may be influenced by GA and the ambient temperature and may thereby affect seed survival are discussed.
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  • 23
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; preharvest sprouting ; dormancy ; α-amylase ; falling number ; inhibitors ; embryo response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Resistance to preharvest sprouting has been observed in several white wheat (Triticum aestivum L.) genotypes, but the mode of resistance has not been determined. Studies were conducted to characterize the strong preharvest sprouting resistance in Clark's Cream genotype to facilitate breeding for the trait. Clark's Cream and five other hard red and white wheat genotypes were grown in the glasshouse and field and effects of simulated rain, embryo maturity, inhibitors in floral parts and GA, endogenous inhibitors, and ABA and GA combinations on sprouting were measured. The six genotypes differed significantly in sprouting after simulated rain, α-amylase activity, and response to GA and ABA. Embryo maturity and inhibition levels in floral parts and caryopses were nearly similar for all genotypes. Clark's Cream appeared to differ most in high embryo sensitivity, which was fairly nonspecific for ABA, GA, and endogenous inhibitor, and in low α-amylase production during sprouting. Breeding procedures that lead to the preharvest sprouting resistance of Clark's Cream are discussed.
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  • 24
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; breeding ; preharvest sprouting ; heritability ; variation ; α-amylase ; falling number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Preharvest sprouting resistance is a major breeding criterion in many regions where white wheat (Triticum aestivum L.) is produced. Availability of genetic sources of sprouting resistance should significantly advance this goal. Objectives of the present investigation were to determine heritabilities and genetic variation of sprouting resistance in progeny of six susceptible genotypes and Clark's Cream, a cultivar with excellent sprouting resistance. Sixty-five randomly selected F2:F3 lines from the six parental combinations were evaluated in blocks-within-replication designs at two locations during 1984–85. Falling number, α-amylase activity, and sprouting percentage in simulated rain were usually more favorable in F2:F4 grain than in grain of the susceptible parents and frequently equalled levels in grain of the resistant parent. Broad sense heritability estimates were moderate to high for falling number and α-amylase activity, low to moderate for visual sprouting, and inconsistent for embryo germination in ABA. Phenotypic correlations with sprouting were significant most often for falling number and least often for the agronomic traits, days to heading and kernel weight. We concluded that increased preharvest sprouting resistance is an attainable objective when genetic sources of strong resistance and appropriate selection criteria, such as sprouting after simulated rain, are used.
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  • 25
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; dormancy ; gibberellic acid ; pre-harvest sprouting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Gibberellic acid, cold (4°C) and a combination of these two treatments were tested for use in breaking dormancy in 27 lines of white-grained wheat with varying levels of resistance to pre-harvest sprouting. Germination increased in all lines treated with gibberellic acid. Dormancy could be broken with 1 μM gibberellic acid. Response to cold varied. A combination of gibberellic acid and cold treatment was the most effective. This technique has been found useful in treating seed in a breeding program aimed at producing wheats with resistance to pre-harvest sprouting.
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  • 26
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    Euphytica 39 (1988), S. 137-144 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum vulgare ; barley ; boron toxicity ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and yield of seven wheat and two barley cultivars or lines, previously found to show different degrees of boron tolerance under field conditions, were compared in a pot experiment at a range of soil boron treatments. Soil treatments ranged up to 150 mg/kg applied B. Extractable B in soils ranged up to 103 mg/kg. At the highest B treatment seedling emergence was delayed, but the percentage emergence was not reduced. The degree of boron toxicity symptom expression varied between the wheat cultivars and lines, with the two most tolerant, Halberd and (Wq*KP)*WmH)/6/12, displaying the least symptoms. The concentration of boron applied to the soil which produced a significant depression of growth and yield varied between cultivars. For example, the yield of (Wq*KP)*WmH)/6/12 was not affected at the 100 mg/kg applied boron treatment, while the grain yield for (Wl*MMC)/W1/10 was significantly reduced at the 25 mg/kg treatment. There was a linear increase in boron concentration in tillers at the boot-stage with increasing concentration of boron in the soil. The most boron tolerant genotypes had the lowest tissue boron concentrations in each of the treatments. Halberd and (Wq*KP)*WmH)/6/12 had approximately half the boron concentrations of the more sensitive genotypes at the 25 and 50 mg/kg treatments. Differential tolerance of boron within the tissue was also observed. Both Stirling and (Wl*MMC)/W1/10 had significantly reduced total dry matter and grain yields at the 25 mg/kg treatment, while the concentrations of boron in boot stage tillers at this treatment were 118 and 100 mg/kg, respectively. On the other hand, Halberd and (Wq*KP)*WmH)/6/12 had tissue boron concentrations of 144 and 131 mg/kg, respectively, at the 50 mg/kg treatment but yield was unaffected. The relative responses in the pot experiment, for wheat, were in close agreement with field results. Halberd and (Wq*KP)*WmH)/6/12 had the highest grain yields, with the lowest concentrations of boron in the grain when grown under high boron conditions in the field. In pots these two genotypes proved to be the most tolerant of boron. For barley the advantage in grain yield in the field, expressed by WI-2584 compared with Stirling, was not repeated in pots. WI-2584 was, however, more tolerant than Stirling on the basis of total dry matter production. The results show that useful variation in boron tolerance exists among wheat, and that breeding should be able to provide cultivars tolerant to high levels of boron.
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  • 27
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    Euphytica 34 (1985), S. 367-370 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; frost resistance ; chromosome 5A ; reciprocal monosomic analysis ; allelic variation ; substitution line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary F2 monosomic analysis and the direct comparisons between aneuploid series from different varieties of wheat suggest the likelihood of allelic variation. It is impossible however, from these studies to prove unequivocally that allelic variation exists. Some effects can be due to chromosome dosage rather than allelic variation. This disadvantage was overcome by using reciprocal monosomic analysis to study the genetic control of frost resistance on chromosome 5A in wheat. Data support the previous results obtained by F2 monosomic and substitution analysis. The chromosome 5A has been shown to be the one which carries the major allelic differences that distinguish wheat varieties Chinese Spring, Rannyaya 12 and Mironovskaya 808 for frost resistance.
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  • 28
    ISSN: 1573-5060
    Keywords: Tricticum aestivum ; wheat ; yield ; genotype x environment ; retrospective ; environments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The identification of relevant but diverse environments for the assessment of the relative performance of wheat cultivars was developed by using conventional and pattern analysis procedures. The utility of weighting environments on proximity to a hypothetical most frequently encountered environment, to improve the quality of the predictive inference of relative cultivar performance, was also assessed. There was an increase in the agreement of the relative yield of cultivars obtained in different years using this technique.
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  • 29
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    Euphytica 36 (1987), S. 831-839 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; honeycomb selection ; breeding methodology ; selection intensity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effectiveness of the honeycomb selection method for yield in spring wheat (Triticum aestivum L.) was evaluated using progenies from two wheat crosses, Glenlea x NB131 and Glenlea x Era. Honeycomb selection was carried out in the F2 and F3 generations, grown at the University of Manitoba in the summers of 1980 and 1981, respectively. In both generations, divergent selection was made for both high and low yield. Plants selected in the F3 generation were entered in an F4 yield test in the summer of 1982. Results of the experiment showed that honeycomb selection for yield in the F2 and F3 generations was effective in identifying parents of high- and low-yielding lines. F3 plants from highyielding F2 selections gave higher yields than those from low-yielding F2 selections by 11.5% and 13.0% for Glenlea x NB131 and Glenlea x Era crosses, respectively. The F4 yield test showed that high yielding selections from both crosses significantly outyielded by 8.9% low yielding selections and by 14.4% the unselected composite lines. It is concluded that the honeycomb selection method can be used for early generation selection in spring wheat.
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  • 30
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    Euphytica 42 (1989), S. 41-44 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; frost resistance ; locus Fr1 ; recombinant lines ; chromosome 5A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A gene for frost resistance on chromosome 5A of wheat was located using single chromosome recombinant lines from the cross between the substitution line Hobbit (Triticum spelta 5A) and Hobbit. In this sample of recombinant lines the locus for frost resistance, designated Fr1, is completely linked to the locus Vrn1 controlling vernalisation requirement. The results can be explained by a pleiotropic action of the Vrn1 locus or close genetic linkage between Vrn1 and Fr1. Further detailed study is necessary to resolve these alternative hypotheses.
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  • 31
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; chromosome substitution ; frost resistance ; hardening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The highly frost resistant wheat variety Cheyenne (donor) and the poorly frost resistant variety Chinese Spring (recipient) were frozen at −9° C and −11° C at various stages of hardening, as were a number of substitution lines of these two varieties (CS/Ch 3A, CS/Ch 5A, CS/Ch 7A, CS/Ch 2B, CS/Ch 4B, CS/Ch 5B, CS/Ch 4D, CS/Ch 5D). Chromosomes 5A, 5B, 5D, 4B and 7A of Cheyenne increased the frost resistance of the recipient variety to varying extents. However, the frost resistance changed not only as a function of the different chromosomes, but also as a function of the duration of hardening, indicating that genes responsible for frost resistance are expressed differently during different phases of the hardening process.
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  • 32
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    Euphytica 39 (1988), S. 7-25 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Triticum aestivum ; wheat ; cytogenetics ; chromosome identification ; gene localisation ; interspecific gene transfer ; chromosome elimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Achievements and limitations regarding three aspects of cytogenetic research in barley and common wheat are illustrated and discussed. Unambiguous chromosome identification has become possible through the application of chromosome banding techniques, mainly C-banding, N-banding and Ag-banding. Gene localisation studies have yielded a vast amount of information regarding the genetic architecture of barley and wheat. Many genes have been allocated to specific chromosomes, and linkage studies have been carried out with some of these genes. There is growing evidence for a considerable discrepancy between distances on the genetic linkage maps and the physical maps of barley and wheat chromosomes. Although barley can be hybridised with most species of the genus Hordeum and with several species of related genera, interspecific gene transfer is very rare and barley breeding can presently make use of the gene pool of only one wild species, viz. H. vulgare ssp. spontaneum. For wheat breeding, the gene pools of species of the genus Triticum and species of related genera are accessible. Several methods have been developed to achieve gene transfer. Genome interactions in interspecific hybrids result in spatial separation of the parental genomes, in nucleolar competition, and sometimes in chromosome elimination and the formation of haploids.
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  • 33
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust resistance ; alien gene transfers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nine transfers of leaf rust (Puccinia recondita Rob. ex Desm.) resistance to wheat (Triticum aestivum L.) from Agropyron elongatum Host. Beauv., Triticum speltoides Tausch and rye (Secale cereale L.) were backcrossed up to 10 times to commercial wheat cultivars. The objective was to study the effect of the transfers on agronomic and quality characters and to make them available in desirable genetic backgrounds. The results varied greatly for different transfers. In four cases no promising material was obtained even after nine backcrosses. However, for the remaining five transfers material with potential as a new cultivar was obtained.
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  • 34
    ISSN: 1573-5060
    Keywords: alloplasmic line ; breeding ; chromosome ; eyespot resistance ; substitution line ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The variety Roazon possesses genes for resistance to eyespot including that on the 7D chromosome transferred from Aegilops ventricosa. In order to assess the level of resistance due to the latter, we developed the intervarietal substitution for the 7D chromosome of ‘Roazon’ into ‘Courtot’ which is very susceptible to eyespot. As ‘Roazon’ contains Aegilops ventricosa cytoplasm, the substitution lines were produced both with wheat and Aegilops cytoplasm. Resistance was assessed at the seedling and the adult plant stage. No cytoplasmic effect was observed. The substitution lines were as resistant as ‘Roazon’. This shows that the chromosome 7D genes for resistance introduced from Aegilops ventricosa result in a high level of resistance in wheat.
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  • 35
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; infection frequency ; urediosorus size ; growth stage ; component analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Latency period (LP), infection frequency (IF) and urediosorus size (US) of leaf rust were determined on primary leaves and young flag leaves of 18 spring wheat cultivars. A large growth stage effect and a large cultivar effect on all three components were observed. Partial resistance as measured by the three components was generally better expressed in the adult plant stage than in the seedling stage. Associated variation of the components was observed: long LP, low IF and small US tended to go together. The association was not complete, cultivars with clear deviations of this association for one of the components were found suggesting the existence of at least partly different genetic factors controlling the respective components. LP measured on flag leaves gave the most reliable results and, therefore, could best be used as a selection criterion in breeding programs for partial resistance.
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  • 36
    ISSN: 1573-5079
    Keywords: awn ; calvin cycle ; C4 pathway enzymes ; CO2 assimilation ; flag leaf ; pericarp ; glumes ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Activities of key enzymes of Calvin cycle and C4 metabolism, rate of 14CO2 fixation in light and dark and the initial products of photosynthetic 14CO2 fixation were determined in flag leaf and different ear parts of wheat viz. pericarp, awn and glumes. Compared to the activities of RuBP carboxylase and other Calvin cycle enzymes viz. NADP-glyceraldehyde-3-phosphate dehydrogenase, NAD-glyceraldehyde-3-phosphate dehydrogenase and ribulose-5-phosphate kinase, the levels of PEP carboxylase and other enzymes of C4 metabolism viz. NADP-malate dehydrogenase, NAD-malate dehydrogenase, NADP-malic enzyme, NAD-malic enzyme, glutamate oxaloacetate transaminase genase, NADP-malic enzyme, NAD-malic enzyme, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase, were generally greater in ear parts than in the flag leaf. In contrast to CO2 fixation in light, the various ear parts incorporated CO2 in darkness at much higher rates than flag leaf. In short term assimilation of 14CO2 by illuminated ear parts, most of the 14C was in malate with less in 3-phosphoglyceric acid, whereas flag leaves incorporated most into 3-phosphoglyceric acid. It seems likely that ear parts have the capability of assimilating CO2 by the C4 pathway of photosynthesis and utilise PEP carboxylase for recapturing the respired CO2.
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  • 37
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    Plant growth regulation 5 (1987), S. 235-239 
    ISSN: 1573-5087
    Keywords: Triticum turgidum ; wheat ; Chlormequat ; CCC ; dwarfing genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two crosses between Triticum turgidum wheat lines differing in their response to chlormequat (CCC) were tested. In the F2 population of one cross, which was segregating for the Rht1 dwarfing allele, each plant was cloned by separation of two tillers, one of which was treated with CCC. The tall (rht1/rht1) and the intermediate (Rht1/rht1) genotypes showed a greater response to CCC than the semi-dwarf (Rht1/Rht1) genotype, as expressed by culm length and date of ear emergence. The F3 families of another cross and their two semi-dwarf parents were grown in a three-replicated field test in paris of rows, one of which was treated with CCC. In one of the parents and in 1/4 of the F3 families CCC induced a wide-angled tiller growth, suggesting a monogenic control of this growth habit in response to CCC.
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  • 38
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    Plant growth regulation 7 (1988), S. 3-17 
    ISSN: 1573-5087
    Keywords: Abscisic acid (ABA) content ; barley ; genetic variation ; grain weight ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two genetically related wheat lines growing in cabinets were given different temperatures during grain filling, and abscisic acid (ABA) was measured in whole grains by gas chromatography with an electron-capture detector. Three genetically related barley lines grown in the field were assayed for ABA content in endosperm and embryo fractions separately by radiommunoassay. Maximum grain growth rate and final weight per grain of the two wheat lines differed by 50–60% at low temperature and 30–40% at high temperature. During grain development two peaks in ABA level were observed at low temperature but only one at high temperature. At times when differences in grain growth rate between genotypes and between temperature treatments were large, the corresponding differences in ABA concentration were small. In barley, one line (Iabo 14) had 30% heavier grains than the other two (Onice and Opale). Endosperm ABA concentrations showed no clear differences between genotypes until grain filling was nearly complete. Embryo ABA levels were up to 10-times greater than those in the endosperm, with Opale having significantly less ABA in the embryo than the other two cultivars. Our experiments did not provide evidence for a causal relationship between ABA levels during grain filling and grain growth rate or final weight.
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  • 39
    ISSN: 1570-7458
    Keywords: Coccinella septempunctata ; Coccinellidae ; wheat ; egg laying ; larva ; adult ; vertical distribution ; Coccinella septempunctata ; Coccinellidae ; blé ; ponte ; larve ; adulte ; répartition spatiale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary At the beginning of spring, post-hibernating adults of Coccinella septempunctata L. (Col., Coccinellidae), larvae and nymphs are found on the lower part of wheat plants. In June, adults born in the field as well as their progeny are dispersed all over the plant and a significant proportion of them is found on the apical parts of plants. Most of the egg clutches are layed on soil lumps, stones and small wild plants which represent preferential sites. Variations in coccinellid distribution may be influenced by climatic conditions, especially the temperature, by the thermophilic nature of this species and by the repartition of the three main aphid species on the plants. These results are relevant for the selection of a sampling methods.
    Notes: Résumé Au début du printemps les adultes de Coccinella septempunctata L. (Col., Coccinellidae) issus des sites d'hivernation, les larves et les nymphes qui en proviennent, fréquentent le sol et les parties basses du blé. En juin, les adultes nés dans le champ et leur descendance sont dispersés sur toute la hauteur des talles bien qu'une fraction non négligeable de leur population se rencontre à l'extrémité supérieure. La plupart des pontes sont déposées sous les mottes de terre, les pierres et les petites adventices qui constituent des sites privilégiés. Ces variations de la distribution de cette coccinelle peuvent être mises en relation avec le climat, en particulier la température, avec la thermophilie de cette espèce et avec la répartition sur les différentes parties des talles des 3 espèces de puceron qui se succèdent au cours de la saison. Ces observations sont importantes à considérer lors du choix ou de la mise au point d'une méthode d'échantillonnage.
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  • 40
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    Entomologia experimentalis et applicata 53 (1989), S. 17-29 
    ISSN: 1570-7458
    Keywords: Mayetiola destructor ; Cecidomyiidae ; oviposition ; host specificity ; wheat ; oats ; insect-plant interactions ; thresholds ; deprivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les réactions de femelles préalablement accouplées de Mouches de Hesse ont été examinées en analysant le comportement de femelles isolées sur blé et sur avoine. Le répertoire comportemental de ces femelles comprenait: le vol, l'atterrissage sur feuille, la flexion du corps de sorte que l'extrémité de l'abdomen touchât la surface de la feuille, l'antennation, les mouvements de l'extrémité de l'abdomen sur la feuille à angle droit des nervures, le repos avec la tarière droite et encore dévaginée, le repos avec la tarière télescopée à l'intérieur du corps. Sur blé plus que sur avoine, les femelles après atterrissage ont présenté une fréquence plus élevée de passage de la flexion à l'antennation que de la flexion à l'abdomen droit. Durant les 5 premières minutes d'observation, les individus libérés dans des enceintes avec blé fléchirent et ‘antennèrent’ 2 à 3 fois plus que ceux libérés sur avoine. Les durées des différentes séquences différaient aussi: sur blé, pendant les 5 premières minutes, les femelles passèrent plus de 50% du temps à fléchir, tandis que sur avoine elles passèrent plus de 50% du temps en repos. Les femelles restèrent aussi plus longtemps sur les feuilles de blé et y pondirent 4 fois plus d'oeufs que sur avoine. Les femelles de M. destructor ont montré une plasticité du seuil d'acceptation. Pendant les premières heures de ponte, elles ont été très sélectives et refusèrent, ou ne pondirent que quelques oeufs sur avoine, mais acceptèrent volontiers le blé. La discrimination s'est poursuivie tant que les femelles ont eu accès au blé en même temps qu'à l'avoine. Cependant, quand les femelles ont été privées de blé pendant plusieures heures, l'acceptation de l'avoine a augmenté. Cet accroissement de l'acceptation a eu lieu à peu près au moment où les femelles sur blé pondaient leurs derniers oeufs.
    Notes: Abstract Responses of mated female Hessian flies were investigated by analysing the behaviour of individual flies in wheat and oats. The behavioural repertoire of such females included: flying, alighting on leaves, arching of the body so that the tip of the abdomen touched the leaf surface, antennation, movements of the tip of the abdomen across the leaf at right angles to leaf veins, sitting with the ovipositor straight but still extended, and sitting with the ovipositor telescoped into the body. After alighting, females on wheat showed a higher frequency of transitions from arching to antennation and a lower frequency of transitions from arching to abdomen straight than females on oats. During the first 5 min of observations, individuals released into arenas with wheat arched and antennated 2–3 times more frequently than females released into oats. Time allotted to behaviours also differed; during the first 5 min, females in wheat spent 50 percent more time arching, whereas females in oats spent 50 percent more time sitting. Females in wheat also stayed longer and laid 4 times more eggs than females in oats. Temporal changes in egglaying were monitored by quantifying hourly rates of egglaying in no-choice assays for several hours following mating at 9:00 am. During the first and second hours post-mating, egglaying occurred infrequently. However, during the third hour post-mating (11:00 am to noon) females on wheat laid 5 times more eggs than females on oats. Rates of egglaying decreased on wheat but increased on oats during the fourth hour, and then during the fifth hour, decreased on both wheat and oats. Changes in egglaying responses were also evident when behaviours of individual females were measured 1–3 h vs. 3–7 h post-mating. Females deprived of host plants and released into wheat or oats later in the day showed higher frequencies of arching and antennation and laid more eggs before leaving the arena.
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  • 41
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    Entomologia experimentalis et applicata 46 (1988), S. 47-54 
    ISSN: 1570-7458
    Keywords: Coleoptera ; Curculionidae ; Sitophilus ; S. oryzae ; S. zeamais ; rice weevil ; maize weevil ; geographical strains ; digestion ; amylase ; diet ; barley ; corn ; maize ; rice ; wheat ; amylase inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé La transformation de rang des valeur d'activité spécifique de l'α-amylase de 4 souches de S. oryzae et de 4 souches de S. zeamais montre que les niveaux les plus élevés de ces enzymes prédominantes s'observent chez les adultes nourris d'orge mondé ou de riz brun á grains longs. Des niveaux intermédiaires d'activité ont été obtenus chez les insectes élevés sur maïs jaune, et les niveaux les plus faibles chez ceux élevés sur blé. Bien que les extraits préparés à partir d'orge présentent une activité inhibitrice de deux isoamylases purifiées de S. oryzae, les niveaux des inhibiteurs naturels α-amylase de ces deux enzymes sont environ respectivement 2,2 et 6,1 fois plus concentrés dans le blé. L'ingestion de ces inhibiteurs d'amylase et la formation d'un complexe enzyme inactive/inhibiteur avec l'amylase secrétée antérieurement, peut rendre compte de la plus faible activité de l'amylase chez les charançons consommant du blé. Le niveau d'amylase de S. oryzae est 2 fois plus élevé que celui de S. zeamais pour toutes les souches élevées sur un régime donné. Des niveaux d'activité significativement différents ont été trouvés suivant les souches pour chacune des deux espèces. Puisque l'amylase est la principale hydrolase digestive de ces espèces, l'intensité de la modification des teneurs en amylase par la consommation de céréales peut indiquer leur adéquation comme hôtes potentiels.
    Notes: Abstract Rank transformation of specific activity values of α-amylase across four strains of Sitophilus oryzae (L.) and four strains of S. zeamais Motschulsky indicates that levels of these predominant enzymes are highest in adults feeding on hulled barley or long-grain brown rice. Intermediate activity levels are found in weevils feeding on yellow corn (maize) and lowest levels are found in wheat-fed weevils. Although extracts prepared from barley contain inhibitory activity against two purified isoamylases from S. oryzae, levels of the naturally-occurring α-amylase inhibitors against these two enzymes are about 2.2-fold and 6.1-fold, respectively, more concentrated in wheat. Ingestion of these amylase inhibitors and formation of an inactive enzyme:inhibitor complex with previously secreted amylase may account for the lower activity of amylase in weevils of both species feeding on wheat. Amylase levels across all strains feeding on a given diet are about 2-fold higher in S. oryzae than in S. zeamais. Significant differences in activity levels were also found between strains in both species. Since α-amylase is a predominant digestive hydrolase in these species, the degree to which cereal diets affect amylase levels may indicate their suitability as potential hosts.
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  • 42
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    Plant and soil 101 (1987), S. 51-60 
    ISSN: 1573-5036
    Keywords: dry matter ; field capacity ; growth function ; nitrogen uptake ; rooting ; sowing date ; thermal time ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The quantities,Y, of nitrogen taken up, and dry matter produced, at various times during the growth of six winter wheat crops at Rothamsted were shown to be related to thermal time,x, based on soil temperature, by a simple equation $$Y = (A^{ - 1/n} + \exp ( - kx))^{ - n} $$ whereA is the ultimate maximum ofY, n a shape factor andk a rate constant that is related toA andn throughx′, the inflexion point of the function. The value ofn was 1.5 for both N uptake and dry matter. The value ofA for N uptake,A N , was well described by a multiple regression on sowing data,t s , expressed as the number of days after August 31st, and rainfall,R Apr , in the April before harvest, but no such regression could be found for the value ofA for dry matter,A D . The rate constants,k N andk D , for N-uptake and dry-matter production respectively, could be related to the date of sowing and the weather through the corresponding inflexion points,x′ N andx′ D . Highly significant regressions were found, forx′ N on the time,t sf , between sowing and the return of the soil to field capacity and forx′ D on the reciprocal oft s . The function was used to generate N uptake curves from values ofA N andk N (obtained fromx′ N ) given by inserting the appropriate values ofR Apr ,t s andt sf in the regressions. These fitted measured N uptakes satisfactorily for the six crops used to obtain the regressions, and four grown subsequently, at Rothamsted, and also for six crops at Woburn. Values ofA D had to be set arbitrarily because no regression had been found to predict them, but using these arbitrary values in the function gave dry matter curves that fitted the measurements satisfactorily for all ten Rothamsted crops and two of the Woburn crops. Tests for seasonal and site effects showed thatA N was influenced more by differences between the two sites than by seasonal differences other than those inR Apr , whereasA D was strongly influenced by these seasonal differencess and very little by those between the sites.
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  • 43
    ISSN: 1573-5036
    Keywords: barley ; Cochliobolus sativus ; common root rot ; subcrown internode ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The progression of common root-rot lesions on subcrown internodes of Neepawa wheat and Bonanza barley plants inoculated 14, 26, 38 and 50 days after seeding was measured in growth chamber tests. Both in wheat and barley, lesion development was more rapid in older than in younger plants. Variables such as mean daily rates of linear progression of lesions, disease ratings, and proportion of plants becoming severely diseased were higher in older than in younger plants. The possible stress resulting from the removal of the crown roots on more rapid disease development in older than in younger plants is discussed.
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  • 44
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    Plant and soil 101 (1987), S. 281-285 
    ISSN: 1573-5036
    Keywords: cabbage ; fertilizer ; granule ; interception ; lettuce ; model ; phosphorus ; root ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A model is described which compares the rate of phosphorus demand of a field grown lettuce crop with the rate at which the crop can obtain phosphorus from broadcast fertilizer. Early root growth of lettuce is so slow that on average it takes about 21 days for the first granule of fertilizer to be exploited by a plant, whereas seed reserves of phosphorus are depleted within about 8 days of sowing. This is likely to result in a reduction in yield which cannot be overcome by simply applying more broadcast fertilizer. Some placement of fertilizer near to each plant is required if phosphorus deficiency is to be avoided. The model can also be applied to other crops, and the delay before the first fertilizer granule is exploited is shown to be slightly shorter in cabbage and very short in wheat.
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  • 45
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    Plant and soil 101 (1987), S. 287-290 
    ISSN: 1573-5036
    Keywords: barley ; common root rot ; infrared thermometry ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In field trials done from 1983 to 1985, two cultivars of wheat and two cultivars of barley were rated periodically for common root rot, and leaf temperatures were recorded with a hand-held infrared thermometer. Significant differences for common root rot occurred between cultivars of both wheat and barley but leaf temperatures did not differ among cultivars. Disease rating and leaf temperature were not correlated in wheat and only 2 of 47 comparisons were positively correlated in barley.
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  • 46
    ISSN: 1573-5036
    Keywords: competition ; nitrogen ; nutrition ; phosphorus ; wheat ; wild oats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the cultivated wheat fields of the Mediterranean area, wild oats (Avena sterilis L.), is commonly found a weed, which competes with the wheat for soil nutrients, water and light. To study this mutual competition for N and P, a pot experiment was devised in which the planting of wheat and weed seeds, in the pots, was so arranged that the lengths of time between the emergence of each specie was different for each treatment. When the time of emergence of the wild oat coincided with that of wheat, the latter was affected in its development, productivity and total accumulation of N and P. However, the earlier the emergence of wheat was relative to that of wild oat, the greater was the suppression of the weed, with a lower total accumulation of N and lower P content in the plant. Bringing forward the emergence of either of the two species conferred advantages for the development of the earliest emerger.
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  • 47
    ISSN: 1573-5044
    Keywords: wheat ; somatic embryogenesis ; embryogenic callus ; 3,6-dichloro-o-anisic acid ; 2,4-dichlorophenoxyacetic acid ; 6-furfurylaminopurine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nine experiments were conducted to determine effects of various culture medium addenda on induction of embryogenic calli from immature embryos of a responsiveTriticum aestivum L. genotype (PCYT 10). Effects were quatified by counting somatic embryos (embryoids) per callus. Optimal auxin concentrations to induce and maintain somatic embryogenesis were 3.62 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 9.05 μM 3,6-dichloro-o-anisic acid (dicamba). In general, dicamba permitted formation of significantly more embryoids than 2,4-D. Kinetin (6-furfurylaminopurine) at 2.56 μM or 4.65 μM significantly increased percentage scutellar callus when added to 2,4-D or dicamba-containing medium, respectively. Kinetin at 4.65 μM signficantly increased the numbers of embryoids formed when added to medium containing either synthetic auxin. Significantly fewer embryoids formed when cultures were incubated under diffuse light (16-h photoperiod). Casein hydrolysate (200 mgl-1) or L-arginine (0.23 mM) had no effect on numbers of embryoids formed, whereas L-tryptophan (0.20 mM) enhanced such formation with 2,4-D and decreased such formation with dicamba. Two additional experiments generally demonstrated that response to auxin source in the genotypes ND 7532, PCYT 20, Yaqui 50, and Oasis was similar to that in PCYT 10. The higher molar concentration of dicamba required to induce embryogenic callus coupled with more evident embryoid precocious germination and a more rapid rate of tissue necrosis upon extended incubation without subculture suggests that dicamba is metabolized more rapidly than 2,4-D inT. aestivum callus cultures.
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  • 48
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    Plant cell, tissue and organ culture 12 (1988), S. 299-304 
    ISSN: 1573-5044
    Keywords: callus ; plant regeneration ; isozymes ; wheat ; barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures fron non-organogenic, young and one-year old, and morphogenic calli were used to assess the value of isozymes analysis for the prediction of morphogenic capacity by studying esterase, peroxidase and acid phosphatase. Basic isozyme patterns of each enzyme for the callus were retained in all the callus stages and in the callus which has differentiated into shoots. With the development of shoot and/or root some conspicuous isozymes appeared for esterase and acid phosphatase and some disappeared for peroxidase. As the isozyme changes became apparent only after shoot or root initiation these enzymes could not be used as markers to distinguish between morphogenic and non-morphogenic calli.
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  • 49
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    Plant cell, tissue and organ culture 7 (1986), S. 31-37 
    ISSN: 1573-5044
    Keywords: wheat ; tissue culture ; callus ; regeneration ; coconut milk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of the interaction of genotype and culture medium on the initiation of callus from immature embryos and subsequent plant regeneration was investigated in eight hexaploid wheat lines. Intervarietal differences in culture response and interaction of the genotype with coconut milk are reported. The relative contributions of media and genotype effects to culture performance are assessed. The observation that primordia and shoot development was promoted by coconut milk in some lines and inhibited in others is particularly significant given that coconut milk is widely used to try to improve culture response. This report shows that this effect is dependent on the genotype of the tissues in culture.
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  • 50
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; growth analysis ; relative growth rate ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary For a better insight in the effect of wide spacing on the outcome of plant selection in spring wheat, the growth of free-grown individual plants was followed in time for each of 12 cultivars. As time proceeded, the cultivar differences for per-plant weight showed progressively less relation with those for seedling weight. At anthesis, the genetic correlation with seedling weight was still about 0.80, but at final harvest it was only 0.06. This contrasted with the genetic correlation between seedling weight and final biomass in closely planted mixtures which was on the average 0.77. The outcome of selection of genotypes in isolation is therefore expected to deviate substantially from that of selection in densely planted segregating populations where the differences in initial size tend to be maintained in time. The ranking of the varieties grown in isolation changed in time because of differences in relative growth rate (RGR). The genetic variance of RGR decreased much less with time than RGR itself. Late-flowering varieties showed the higher RGR so that these varieties improved their position in the ranking in isolation. Special attention is paid to the methodology of plant growth analysis in variety experiments and in estimating means and variances of RGR.
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  • 51
    ISSN: 1573-5060
    Keywords: Trticum aestivum ; wheat ; gram yield ; grain protein ; seed quality ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat (Triticum aestivum L.) kernel weight is an important yield component and seed quality factor that appears to be declining with recent cultivar releases in the major U.S. wheat region. The objectives of experiments were to detmrmine the relationship between planted and harvested 1000-kernel weights and their effect on grain yield and grain protein percentage. Twelve popular hard red winter wheat cultivars were grown with recommended practices at 10 Kansas locations for three years. Rank correlation coefficients between kernel weights and grain yields and protein percentages were calculated. Correlations of planted and harvested kernel weights over all cultivars with grain yield were inconsistent, being positive for some locations and years and negative for other locations and years. Planted kernel weights and grain yields of individual cultivars were not related, but harvested kernel weights and grain yields were correlated positively for eight of the 12 cultivars. Harvested kernel weights and grain protein percentages frequently were correlated positively at individual locations, but always were correlated negatively for individual cumtivars. It was concluded that high stable harvested wheat kernel weight is important as a component of grain yield under a range of environments and that improving this trait would benefit all segments of the wheat industry.
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  • 52
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Triticum turgidum ; durum wheat ; Secale cereale ; rye ; x Triticosecale ; 6 x-triticale ; aspartate aminotransferase ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The electrophoretic isozyme phenotypes for the AAT-2 and AAT-3 regions of leaves of Triticum turgidum, T. aestivum, Secale cereale, x Triticosecale (hexaploid) and T. aestivum/ S. cereale 6R addition line are described. The phenotypes varied in distribution and relative intensity of the isozyme bands, which were densitometrically measured. The results are consistent with a hypothesis of the dimeric structure for the AAT-2 and AAT-3 systems.
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  • 53
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    Euphytica 35 (1986), S. 225-232 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; wheat-rye derived cultivars ; dough stickiness ; dough mixing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary During test baking, the wheat line QT2870, bred from a rye-derived parent, Kavkaz, and having the pedigree Kavkaz/Timgalen//3*Oxley, showed excessive dough stickiness when slightly overmixed. Conventional quality tests on QT2870 and three commercial varieties did not show any major differences which could explain this dough stickiness. However, resistogram data for QT2870 were considerably different from those for the other cultivars. The curves for QT2870 had lower breaking points and sharper curve angles, indicating that it had a lower tolerance to high speed mixing than the other varieties. Dough stickiness and a lack of mixing tolerance are likely to be major problems limiting the use of rye-derived parents in Australian bread wheat breeding programmes.
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  • 54
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia graminis tritici ; stem rust ; Puccinia recondita tritici ; leaf rust ; rust resistance ; seedling resistance ; adult-plant resistance ; genetic linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seven genes, viz. Sr5, Sr6, Sr7a, Sr8a, Sr9b, Sr12 and Sr17 were associated with seedling resistance to Puccinia graminis tritici in Kenya Plume wheat. The predominant field cultures were avirulent on seedlings with Sr7a, but possessed virulence for the other six genes. However, Sr7a did not confer adult-plant resistance when present on its own. Adult-plant resistance was attributed to Sr2 and possibly also to the interaction of Sr7a and Sr12. Two genes, Lr13 and Lr14a, were identified in seedling tests with various cultures of Puccinia recondita tritici. Lr13 conferred adult-plant resistance to the predominant field strains. Genetic recombination between Lr13 and Sr9b was estimated at 17.6±3.1%.
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  • 55
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    Euphytica 35 (1986), S. 273-292 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; growth analysis ; relative growth rate ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twelve spring wheat cultivars were grown as isolated plants in the field and their pre-anthesis growth was interpreted in terms of plant growth analysis. Relative growth rate (RGR) decreased steadily with time due to a decline of leaf area per unit plant weight (LAR), which could be explained by the reduction of the portion of leaf weight in total plant weight (LWR). Growth per unit leaf area (NAR) and leaf area per unit leaf weight (SLA) changed only little with time. Differences between cultivars for NAR and LAR were of similar magnitude: both 8% when measured by the genetic coefficient of variation. Because both quantities were negatively correlated, the genetic variation of RGR was only 5%. Genetic variation for LWR and SLA were also of similar size, both about 4%. Estimates of genetic variances and covariances based on cultivar means appeared to be biased strongly when the error variation of the means was neglected. Special attention is paid to the methodology of plant growth analysis.
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  • 56
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Triticum turgidum ; durum wheat ; nulli-tetrasomic lines of wheat ; malate dehydrogenase isozymes ; chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This paper describes the phenotype for MDH-2 isozymes of leaf tissues of Triticum turgidum, T. aestivum and the nulli-tetrasomic lines of homoeologous group 1 of T. aestivum. The results obtained support the hypothesis that the MDH-2 isozymes are monomers coded by genes located in the 1A, 1B and 1D chromosomes.
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  • 57
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    Euphytica 35 (1986), S. 593-602 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; stripe rust ; yellow rust ; Australia ; New Zealand ; numerical classification ; ordination ; minimum spanning tree
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seedling and field reactions to four European races of stripe rust were determined for 254 wheat cultivars, mostly from Australia and New Zealand. Numerical analyses of the data employed a divisive classification procedure with termal reallocation to form 10 groups which were then further classified, ordinated and diagnosed by several procedures. The groups formed ranged from one comprising four wheats with low seedling reactions to all four rust strains and mean field rust of only 1.0%, to one comprising 105 wheats with high seedling reactions and mean field rust of 64.7%. Groups of cultivars with intermediate levels of rust resistance were classified both on relative level of overall rust and on differential reactions to rust strains. One group of 13 cultivars had moderately high seedling reactions but averaged only 1.5% rust in the field. Seedling response to gibberellic acid (GA) was also measured and diagnosed as an external attribute, along with grain type and region of origin. Principal co-ordinate analysis revealed that greater resistance to stripe rust was associated with the insensitivity to GA typical of semidwarf wheats and with an origin in northern Australia, where semidwarf wheats have been most used in the breeding program. In terms of numerical analysis of disease data in plant breeding programs, the study highlighted the advantages of using divisive classification with terminal reallocation of group members.
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  • 58
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    Euphytica 35 (1986), S. 523-528 
    ISSN: 1573-5060
    Keywords: Triticum aeastivum ; wheat ; chlorophyll synthesis ; telocentric analysis ; inheritance ; location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An Indian hexaploid wheat var. Pb C591 has been shown to carry gene(s) for chlorophyll synthesis on chromosome 3A (Singh & Joshi, 1979). In the present study cv. Pb.C591, its monosomic 3A and diteocentrics for 3Aα, 3BL and 3DL of var. Chinese Spring have been used. The F2 segregation involving crosses between Pb.C591 as male, monosomic line 3A of Pb.C591 (female) and ditelocentrics 3Aα, 3BL and 3DL of cv. Chinese Spring as male and female respectively has been observed. It has been found that there are two dominant genes regulating chlorophyll synthesis in cv. Chinese Spring. These genes are located on chromosomes arms 3Aβ and 3DS respectively. These chlorophyll synthetic genes must be the same which were postulated by Sears (1956, 1957) as the normal alleles of virescent gene v 2 (which was located on 3BS) on chromosomes 3A(v 1) and 3D(V 3).
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  • 59
    ISSN: 1573-5060
    Keywords: Triticum speltoides ; Aegilops speltoides ; Triticum aestivum ; wheat ; Schizaphis graminum ; greenbug ; wheat streak mosaic virus ; insect biotypes ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetic studies were conducted to determine the inheritance of biotype E greenbug resistance in CI 17882 (CI 15092/T. speltoides//Fletcher/3/4* Centurk), a wheat germplasm line previously released as resistant to wheat streak mosaic virus (WSMV). In addition, the association of greenbug and WSMV resistance in CI 17882 was examined. Results indicated that biotype E greenbug resistance in CI 17882 is conditioned by a single dominant gene that is not linked with the WSMV resistance gene.
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  • 60
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    Euphytica 35 (1986), S. 621-629 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; male sterility induction ; gametocide ; benzotriazole ; cupferron ; neocuproine ; cuprizone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Because copper is extremely important to the development of normal polllen, an attempt was made to induce male sterility in wheat by applying specific copper-binding ligands to wheat plants. Four different chelates were used at two rates in three methods of application. All four chelates, cupferron, neocuproine, benzotriazole and cuprizone, reduced grain yield at high concentration applied to the soil at sowing but benzotriazole was most effective, even when applied at late tillering to either soil or foliage, and it also reduced yield to a lesser extent when applied at low concentration. At high concentration of benzotriazole (50 mg kg-1 of dry soil) the percentage of pollen staining with I2/KI was very low (0–7%) depending on method of chelate application), and this soil treatment resulted in complete male sterility. The appearance of the pollen, anthers, grain, ears and leaves in many cases mimicked that of normal copper deficiency, and also that caused by other recognised gametocides. These results raise the question of whether binding of copper or some other disturbance of copper metabolism may be the mechanism by which andro-gametocidal chemicals work and if so, dictate a theoretical basis for selecting such chemicals for testing.
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  • 61
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; grain yield ; selection ; simple mass selection ; gridded mass selection ; rhombic grid selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An experiment was conducted in Winnipeg, Manitoba, Canada to: (1) ascertain the efficiency of gridded mass selection; (2) to determine if additional selection efficiency can be achieved through refinement of the gridded mass selection procedure; and (3) to determine if the degree of homozygosity has an effect on response to single-plant selection. Three selection methods [simple mass selection (SMS); gridded mass selection (GMS); and a new rhombic grid selection (RGS)] were applied to four populations [a conventional F2 (CON-F2); an intermated F2 (INT-F2); and 2 partial backcrossed populations] developed from a biparental cross involving the Canadian spring wheat cultivars, ‘Glenlea’ and ‘Sinton’. Averaged over the 4 populations, a single cycle of RGS (10% selection intensity) increased yield by about 9.5%, whereas GMS and SMS increased yield by approximately 4.5% and 3.1%, respectively. The degree of homozygosity of individual plants that make up the population appeared to have an effect on the efficiency of selection. In general, selection was more efficient in the two partial backcrossed populations, and least efficient in the CON-F2 and INT-F2 populations. The largest response to selection (15.9%) was attained when RGS was applied in the backcross to Glenlea population, and the lowest response (1.2%) when SMS was enforced in the CON-F2 population. This paper represents the first report on the efficiency of rhombic grid selection for yield improvement in cereal crops.
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  • 62
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    Euphytica 44 (1989), S. 143-150 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Triticum durum ; durum ; environmental correlations ; grain yield ; plant height ; tiller number ; grain size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Environmental correlation coefficients were computed among all pairs of five traits, namely grain yield, heading date, number of tillers per m2, plant height and 1000-grain weight (grain size) using 30 Triticum durum and 30 Triticum aestivum varieties grown in 18 environments. Grain yield was significantly correlated with the other four traits in almost all of the varieties. The mean correlation coefficient over all varieties ranged from 0.58 to −0.83 for durum wheat and 0.66 to 0.88 for aestivum wheat. The correlation coefficients between heading date and the other traits were also significant, ranging from −0.45 to −0.79 in durum wheat and −0.61 to −0.85 in aestivum wheat. The correlation coefficient between number of tillers with plant height and 1000-grain weight were the smallest, 0.19−0.32 in durum wheat and 0.39−0.60 in aestivum wheat. It was concluded that agronomic practices favouring early and good stand establishment in the dry regions will favour the yield components and important adaptive traits, which contribute towards larger yields. Significant differences were found among genotypes in the environmental correlation coefficients and the associated changes in one trait as a result of changes in other traits.
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  • 63
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; oligogenic inheritance ; transgressive segregation ; number of genes ; heritability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three partially resistant spring wheat cultivars, with a long latency period were crossed among each other and with the highly susceptible Little Club, with a very short latency period. Parents, F3 and F5 plants have been inoculated with the leaf rust race Flamingo in the young flag leaf stage to determine the latency period. From the crosses with Little Club, it was concluded that Westphal 12A carries three, Akabozu two and BH 1146 two or three genes for a longer latency period. BH 1146 appears to carry also one hypersensitive resistance gene. Transgressive segregation occurred in crosses between partially resistant cultivars. From crosses between the partially resistant cultivars, it was concluded that the genes in Akabozu and Westphal 12A are different, while those in Akabozu and BH 1146 are at least partly different. The possibilities of accumulation of LP-prolonging genes are discussed.
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  • 64
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; temperature sensitivity ; latency period ; infection frequency ; urediosorus size ; growth stage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three components of partial resistance (PR) were studied at three post-infection temperatures using seven spring wheat genotypes differing in level of PR and two different wheat leaf rust races. The components were latency period (LP), infection frequency (IF) and urediosorus size (US). The expression of LP was more sensitive to temperature than the expression of the other two components. LP-prolonging genes were better expressed at low temperatures than at high temperatures and cultivar differences tended to increase with decreasing temperature in both seedling and adult plant stages. The reaction of IF to temperature differed from that of LP and US, probably because IF is regulated by another mechanism than LP and US. It is recommended to perform PR-screening tests at low rather than at high temperatures. If temperatures are maintained at about 8–13°C (night-day), seedlings can be used to screen for PR instead of the more expensive adult plant tests. The effectiveness of PR in seedling stage at low temperatures suggests that the seedling stage may have epidemiological significance as the low temperatures (8–13°C) are relevant for seedlings in the field.
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  • 65
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    Euphytica 44 (1989), S. 273-282 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; race-specificity ; Puccinia recondita f.sp. tritici ; wheat leaf rust ; partial resistance ; hypersensitive resistance ; latency period ; disease severity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Partial resistance (PR) in wheat to wheat leaf rust (Puccinia recondita f.sp. tritici) is characterized by a slow epidemic build-up despite a susceptible infection type. Two greenhouse tests and two field tests, in which 11 spring wheat cultivars were exposed to five wheat leaf rust races, revealed some indication for race-specificity of PR. In the greenhouse, the expression of PR was highly dependent on the environment. Significant cultivar-race interactions in the first experiment were lost in the second experiment probably due to cultivar-environment and cultivar-race-environment interactions. In the polycyclic field tests several factors played a role in explaining the inconsistency of the cultivar-race interactions, such as differences in initial inoculum, genotypic differences in earliness, interplot interference or environmental conditions. One cultivar-race combination showed a significant but small interaction towards susceptibility in both field experiments. The interaction was probably too small to detect in the monocyclic greenhouse tests. The results do not conflict with the idea that a gene-for-gene relationship could exist between PR-genes in the host and genes in the pathogen. Some problems with regard to the selection of PR in wheat to wheat leaf rust are discussed.
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  • 66
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; dwarfing genes ; drought stress ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Near-isogenic tall (no dwarfing gene), semidwarf (Rht1 or Rht2) and dwarf (Rht1 + Rht2 or Rht3) spring wheat lines were evaluated for yield and yield components under irrigated and rainfed conditions. Under irrigated conditions, the dwarf and the semidwarf lines exhibited a significant yield advantage over the tall lines. Under rainfed conditions, the semidwarf lines outyielded the tall as well as the dwarf lines. Percent yield reduction in response to drought stress was highest with the dwarfs and lowest with the tall lines. Dry matter production of the tall lines and that of the semidwarf lines did not differ significantly and both produced significantly more dry matter than the dwarf lines under irrigated as well as rainfed conditions. Plant height and kernel weight decreased with increasing degree of dwarfness while number of kernels per spikelet, harvest index and days to heading increased under both moisture regimes. The dwarfing genes did not have any significant influence on number of tillers/m2 and spikelets per spike in either moisture regime.
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  • 67
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    Euphytica 34 (1985), S. 273-277 
    ISSN: 1573-5060
    Keywords: Triticum durum ; wheat ; clonal propagation ; cytokinins ; shoot proliferation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Immature embryos from five durum wheat cultivars were grown on Murashige and Skoog medium supplemented with two concentrations of kinetin or 6-benzylaminopurine (BAP). The embryos cultured on the medium containing 5 mg/l of BAP proliferated several axillary shoots. Shoot base segments subcultured on the same medium gave more shoot proliferation. The shoots developed into ear-bearing plants. This technique could be used for clonal propagation of wheat.
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  • 68
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    Euphytica 40 (1989), S. 103-109 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; triticale ; wheat-triticale hybrids ; Secale cereale ; rye ; Gaeumannomyces graminis ; take-all ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifteen triticale and wheat-triticale hybrid lines were evaluated for resistance to the take-all fungus Gaeumannomyces graminis var. tritici and compared with five wheat and two rye lines in inoculated field and pot trials. The triticale and wheat-triticale hybrid lines varied in rye chromosome number and degree of resistance expressed. One line, Venus with seven pairs of rye chromosomes consistently showed levels of resistance intermediate between wheat and rye. A trend was observed where increasing rye chromosome content led to greater resistance but exceptions showed that variation within triticales could not be ascribed to rye chromosome content alone.
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  • 69
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; reciprocal F1 monosomics ; ear emergence ; number of leaves ; number of spikelets ; photoperiod ; vernalization ; growth rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A reciprocal F1 monosomic analysis of chromosomal differences between Spica and Bersée was carried out under controlled environment conditions. Chromosomes associated with differences in days to ear emergence, number of leaves and number of spikelets were identified. The results indicated that chromosome 2B of Spica carries a photoperiod insensitivity allele at the Ppd 2 locus. Both Spica and Bersée appear to have a vernalization insensitity allele at the Vrn 2 locus on chromosome 5B. On chromosome 3A, 4B, 4D and 6B factors were found with major effects on earliness per se, diffeences in ear emergence and number of spikelets which were independent of photoperiod and vernalization. The possibility that these factors influence growth rate is discussed.
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  • 70
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    Euphytica 34 (1985), S. 233-236 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; auricle colour ; genetics ; instability of expression ; gene penetration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Only a few publications deal with the genetics of auricle colour of wheat. They report that red pigmentation is conditioned by one dominant gene, symbolized Ra. Ra is present in Egyptian local wheat cultivars. My own research showed the great instability of this character.
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  • 71
    ISSN: 1573-5079
    Keywords: Chlorophyll a fluorescence ; photoacoustic spectroscopy ; photosystem I ; photosystem II ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Usisng intact leaves, the extent of the decrease in chlorophyll a fluorescenece caused by the addition of continuous 710 nm light superimposed on modulated (20 Hz) 550 nm light was used to determine the distribution of this absorbed light between photosystems I (α) and II (β). The Fo and Fm levels, which defined the total variable fluorescenece, were taken as equal to those obtained with excess 710 nm light and with saturating blue-green light, respectively. An analogous procedure was used with a photoacoustic detector, saturating white light defining a base line for oxygen yield, the levels with an without 710 nm light being used to define β and α respectively. The two methods gave similar values for the distribution of light between the two photosystems for the experimental conditions used, β averaging 0.55 for a range of Triticum genotypes and Brachypodium sylvaticum grown in high or low light.
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  • 72
    ISSN: 1573-5079
    Keywords: flag leaf ; grain filling ; photosynthesis ; SDW index ; senescence onset ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationships between photosynthetic capacity and dry matter accumulation during the grain filling period have been studied in flag leaves of Triticum aestivum L., cv. Kolibri grown in Mediterranean field conditions. Particular importance has been given to assimilate accumulation in relation to the onset of senescence. During grain filling, the time course of specific dry weight (SDW) was similar in the blade and in the sheath. Variations in SDW were about six times larger in the sheath than in the blade. Minimum blade SDW values occurred during heading and at anthesis. Maximum blade SDW values were observed two weeks after anthesis. After this, SDW values decreased sharply. The dry matter increase per grain in the period from two weeks after anthesis to the end, was only about 25% of final grain dry weight. The importance of environmental constraints on maximum SDW values are discussed. Maximum SDW values occurred at the beginning of the period of rapid decline in blade net CO2 assimilation rate and leaf nitrogen content, that is, at the beginning of senescence. On the other hand, the stomatal resistance to CO2 and the development of senescence are not apparently related. The maximum blade dry weight increase (considering a value of zero at heading) was about 60 mg dry weight per g fresh weight. The possible relationships between dry matter accumulation and senescence onset are discussed.
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  • 73
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    Euphytica 35 (1986), S. 1045-1051 
    ISSN: 1573-5060
    Keywords: Hordeum bulbosum ; Triticum aestivum ; wheat ; triticale ; haploids ; doubled haploids ; crossability ; seed set ; embryo regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An attempt was made to produce doubled haploids on 16 winter wheat and six spring and winter triticale genotypes thought to carry genes for interspecific incompatibility. The potential for haploid production was maximized by the use of Hordeum bulbosum genotypes selected for high crossability on crossable wheat genotypes, the use of two post-pollination applications of gibberellic acid and by the pollination of immature florets. A low frequency of seed was set on both the wheat and the triticale genotypes, having mean seed sets of 0.20 per cent and 0.27 per cent respectively. Although the frequency of embryos (‘seed quality’) was high, doubled haploid production was further limited by poor embryo differentiation and regeneration. Haploid plantlets were obtained from the wheat cultivars Moulin and Renard, although successful chromosome doubling and doubled haploid production was achieved in Moulin only.
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  • 74
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    Euphytica 34 (1985), S. 559-571 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; ear emergence ; number of leaves ; number of spikelets ; photoperiod ; vernalization ; geographical distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences in response to photoperiod and vernalization and genetic variation independent of photoperiod and vernalization (earliness per se), affecting time of ear emergence of wheat, were identified in controlled environment experiments with 33 varieties of diverse geographical origin. The results were compared with an analysis of time of ear emergence of 10409 T. aestivum accessions from the USDA Small Grain Collection grown from autumn sowings in Pendleton, Oregon, and spring sowings in Fargo, North Dakota. The effect of differences in photoperiod and vernalization sensitivity on time of ear emergence was similar to the effect of earliness per se, both under controlled environment conditions and in the field. Most of the accessions from low latitude regions reached ear emergence rapidly owing to their insensitivity to photoperiod and vernalization and earliness per se factors accelerating ear emergence. Lateness was common among accessions from Northern Europe, Afghanistan and Turkey, which was due to sensitivity to photoperiod and vernalization, and to earliness per se factors delaying ear emergence. The physiological basis of earliness per se is discussed.
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  • 75
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    Euphytica 36 (1987), S. 513-519 
    ISSN: 1573-5060
    Keywords: Tricticum aestivum ; wheat ; genetic analysis ; vernalization inhibiting genes ; Vrn genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study of the Vrn genotypes of 642 spring wheats supports the theory that only Vrn1, Vrn2 and Vrn3 exist in Tricticum aestivum. In none of the varieties investigated Vrn4 was present. Seven varieties, which according to literature carry Vrn4, showed to carry Vrn1, Vrn2 and/or Vrn3. Some varieties were mixtures of Vrn-genotypes, which could mislead geneticists in pooled data analysis. Other causes for misinterpretation of the data could be hybrid necrosis, hybrid dwarfness or a wrong determination of plants with a winter habitus. Only Hope was dominant on another Vrn locus. Its haploid Vrn-genotype is Vrn1 vrn2vrn3 Vrn5.
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  • 76
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    Euphytica 38 (1988), S. 105-111 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; tillering ; heritability ; gene effects ; epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effective utilization of uniculm wheat (Triticum aestivum L.) germplasm in breeding programs requires an understanding of the inheritance of the uniculm character. The parents, F1, F2, and first generation backcrosses (B1 and B2) of two crosses, each utilizing a uniculm spring wheat line and a locally adapted winter wheat cultivar, were space planted in an experiment to acquire information regarding the genetic control of culm number. Significant F1 deviations from midparent values revealed the presence of substantial non-additive gene effects. The scaling tests of Mather and the joint scaling test detected the presence of epistasis. Hayman's six-parameter model revealed that a negative dominance effect provided the major contribution to variation in culm number, while additive x additive and dominance x dominance gene effects were of considerable importance.
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  • 77
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; gene action ; oligogenic inheritance ; transgressive segregation ; number of genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made between the highly susceptible Little Club and the partially resistant cultivars Westphal 12A, Akabozu and BH 1146 to obtain F1, F2 and backcross generations. Latency period (LP) was determined in plants inoculated at the young flag leaf stage with a monospore culture of race ‘Flamingo’ of wheat leaf rust. Broad sense heritability of LP in the F2 averaged 0.8. The genes showed partial to almost complete recessive inheritance. Scaling tests indicated that additive gene action was the most important factor in the inheritance of partial resistance. The tests showed that there were no indications for additive x additive, additive x dominance or dominance x dominance interactions. The number of effective factors was estimated as one or two for Akabozu, three or more for Westphal 12A, and two or three for BH 1146. BH 1146 also possessed a (semi-)dominant gene for a lower infection type which was temperature sensitive in its expression. The genes of the various parents had unequal effect on LP.
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  • 78
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; anther culture ; callus initiation ; cytoplasm ; cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nuclear and cytoplasmic factors affect tissue culture response in wheat (Triticum aestivum), and cytoplasmic male sterility may enhance callus initiation in anther culture. Three wheat nuclear genotypes, each in normal and two alien cytoplasms conferring cytoplasmic male sterility, were evaluated for callus initiation frequency in anther culture. Nuclear genotype had the greatest effect on callus initiation, but cytoplasm and nucleus X cytoplasm interaction also produced significant effects. The nuclear genotype of ‘Chris’ outperformed ‘Butte’ and ‘Coteau’ in all cytoplasms. Ordinary wheat and Triticum timopheevi cytoplasms outperformed Aegilops speltoides cytoplasm. Cytoplasmic male sterility did not increase callus initiation. This suggests manipulating the nuclear genotype is the best strategy for improving the capacity of wheat to initiate callus in anther culture.
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  • 79
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    Euphytica 44 (1989), S. 241-245 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; partial resistance ; environmental stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five spring wheat cultivars differing in partial resistance (PR) to wheat leaf rust were tested at Wageningen (the Netherlands) on a sandy and a clay site, El Batan (CIMMYT, Mexico) and Ponta Grossa (Brazil) over two years. The cultivars were Skalavatis 56, Little Club (both very susceptible), Westphal 12A, Akabozu and BH 1146 (all three with high levels of PR). The results showed that PR was expressed at all four locations in both years. The level of expression was influenced by the environment but the cultivar ranking was hardly affected. Selection for PR in the field can therefore be carried out over a wide range of environments.
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  • 80
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    Euphytica 44 (1989), S. 247-258 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; epidemiological parameters ; microfield ; race nursery ; partial resistance ; components of resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eighteen spring wheat cultivars were tested in microfields and race nurseries for their partial resistance PR to wheat leaf rust under low and high disease pressure respectively. Large differences existed between the 18 cultivars, Skalavatis 56 being the most susceptible and Ponta Grossa 1 being the most resistant cultivar. Of the three epidemic parameters, disease severity (DS) at the time that the susceptible check was severely diseased and area under the transformed disease severity curve (AUTC) and the logistic growth rate (r), AUTC and DS were highly correlated. Both seemed to be reliable estimators of PR but DS should be preferred for economical reasons. The logistic growth rate seemed to be unsuitable as an estimator of partial resistance. High and low disease pressure gave similar cultivar ranking. PR can be screened and selected equally well in race nurseries with low space, low time and low cost input as in microfields with high space, time and cost input. Cultivar differences in development rate had a large impact on the cultivar differences for amount of disease and can therefore greatly bias the estimation of cultivar resistance. The resistance of early cultivars tended to be underestimated whereas the resistance of late cultivars tended to be overestimated. The effect of differences in developmental rate was most pronounced in the flag leaf. It is advisable to avoid the assessment of disease levels on the flag leaf only and to incorporate in the tests several susceptible and resistant checks that cover the range of development rates in the material to be selected, because otherwise selection for resistance will tend to select also for lateness. Regression of the epidemiological parameters on three components of partial resistance revealed that latency period (LP) is an important factor in determining the resistance observed in the field explaining on average 67% of the observed variation. Adding infection frequency (IF) and urediosorus size (US) to the linear model increased the proportion of the observed variation in the field explained by the components to 80%. This result supports the idea that the components of PR inherit independently, at least, in part.
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  • 81
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; gliadins ; electrophoresis ; cultivar identification ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The process of the separation of gliadin proteins of wheat, using polyacrylamide gel electrophoresis, was studied in detail. It was shown that electrophoresis is a dynamic process during which the pH of the gel changes together with the potassium ion concentration. The most positive effect on the separation of the gliadins was generated by a moving front, which is a boundary between regions with a low concentration of K+ ions and a low pH, and region with a high concentration of K+ ions and a high pH, after optimization of the concentrations of the cations and anions in the electrode solutions. The finding was exploited for the development of an extremely simple electrophoresis system, in which buffers were not needed for obtaining a high resolution. The system was further improved by applying a stacking gel. The advantages of this system are discussed. This new approach may be useful for improving electrophoresis systems for other applications.
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  • 82
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    Euphytica 34 (1985), S. 153-159 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; Triticosecale ; callus culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Callus cultures were initiated from inflorescence explants of wheat, rye and triticale on MS medium supplemented with 2 mgl-1 2,4-D+5% CW or 2 mgl-1 2,4-D+0.5 mgl-1 BA. On transfer of the cultures to medium supplemented with 15% CW+0.2 mgl-1 NAA or 1 mgl-1 BA+0.1 mgl-1 IAA, shoot buds and embryoids were produced. Full fledged plantlets obtained on MS medium supplemented with NAA were transferred to the field. Cytological analysis showed the plants to be diploid. However, the regenerated plantlets were shorter, produced fewer tillers and had lower fertility compared to the control.
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    Euphytica 34 (1985), S. 207-211 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; hybrid necrosis ; intergeneric cross
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An inbred line of rye (Secale cereale L.) has been found to carry a gene for hybrid necrosis. This gene was detected in crosses with a highly crossable wheat (Triticum aestivum L.) genotype which carries the gene Ne2. This appears to be the first report of a gene for hybrid necrosis being present in the rye genome.
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  • 84
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    Photosynthesis research 14 (1987), S. 259-267 
    ISSN: 1573-5079
    Keywords: high temperature-induced alterations ; photochemical activities ; post stress reversal ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplasts isolated from elevated temperature treated 8-day-old continuous-white-light-grown wheat primary leaves lost the ability to photo-oxidize water. Also, the ability of ascorbate to donate electrons to photosystem II declined. However, a significant increase in reduced dichlorophenolindophenol-supported photosystem-I-mediated methylviologen photo-reduction activity was observed. The plants stressed at 45°C and 47°C were subsequently grown at 25°C and the partial photochemical activities were measured in chloroplasts isolated from the plants at 24-h intervals. The post stress alterations observed are (1) a significant restoration of water oxidation capacity in 45°C- and partial restoration in 47°C-treated leaves. Ascorbate-supported photochemical activities recovered more or less in similar fashion; (2) reversal of enhanced photosystem I activity in both 45°C- and 47°C-treated leaves. These results suggest that the restoration in water oxidation capacity is possible in 45°C-treated leaves and is limited by the severity of heat stress in 47°C-treated leaves. Restoration of water oxidation capacity vis-à-vis to the reversal of heat-enhanced photosystem I activity also indicates the existence of possible endogenous control for repair of alterations during the post stress.
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  • 85
    ISSN: 1573-5028
    Keywords: mitochondrial DNA ; transfer RNA genes ; rearrangement ; recombination ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the course of isolating tRNA genes from wheat mtDNA, we have found the same tRNAPro gene in two different Hind III restriction fragments, H-P1 (0.7 kbp) and H-P2 (1.7 kbp). Sequences immediately flanking these duplicate genes are closely related, although not identical; sequence comparisons suggest that multiple rearrangements have occurred in the vicinity of the H-P2 tRNAPro gene, relative to the H-P1 version. The chimeric nature of H-P2 is emphasized by the presence of sequences that are also found upstream of the wheat mitochondrial 26S rRNA gene, as well as sequences derived from chloroplast DNA. Comparison of H-P2 with H-P1 plus upstream sequences provides some insight into possible molecular events that might have generated H-P2. In particular, such comparisons suggest a model in which the homologous sequences in H-P2 are seen to be derived from H-P1 plus upstream sequences as a result of an intragenomic, site-specific rearrangement event, followed by amplification of the product, its fixation in the mitochondrial genome, and subsequent sequence divergence (single base changes as well as insertions/deletions of up to 50 nucleotides). The results reported here implicate particular primary sequence motifs in certain of the rearrangements that characterize H-P2.
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  • 86
    ISSN: 1573-5028
    Keywords: chloroplast DNA ; cytochrome b-559 ; photosystem II ; psbL ; wheat
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    Topics: Biology
    Notes: Abstract The N-terminal amino acid sequence of a 3.2 kDa photosystem II polypeptide is shown to be identical to that of a polypeptide encoded by an open reading frame of 38 codons (orf38) in wheat chloroplast DNA. Orf38 is located just downstream of the psbE and psbF genes for the polypeptides of cytochrome b-559. Analysis of the transcription of this region of chloroplast DNA shows that psbE, psbF and orf38 are co-transcribed to give a 1.1 kb polycistronic transcript which also contains another open reading frame of 40 codons. The orf38 and orf40 products are hydrophobic polypeptides which are both predicted to span the thylakoid membrane once. Orf38 and orf40 are highly conserved, and map to similar locations adjacent to psbE and psbF, in all organisms from which this region of DNA has been sequenced. We propose that orf38 is named psbL.
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  • 87
    ISSN: 1573-5028
    Keywords: aleurone ; barley ; protoplasts ; transient expression ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methods have been developed for the isolation of aleurone protoplasts from developing caryopses of Hordeum vulgare and Triticum aestivum in order to study transient expression of introduced genes. Chimaeric gene constructs were introduced into aleurone protoplasts by polyethylene glycol (PEG). Transient expression directed by the 35S promoter from cauliflower mosaic virus (CaMV) of the reporter gene encoding chloramphenicol acetyl transferase (CAT) was detected in aleurone protoplasts from developing barley and wheat grains. Using a similar construct, CAT activity increased when the alcohol dehydrogenase intron 1 fragment from maize was ligated between the 35S promoter and the CAT coding region. The demonstration of transient expression in protoplasts from developing aleurone layers indicates that they may be useful for investigating tissue and developmental control of genes coding for cereal seed proteins.
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  • 88
    ISSN: 1573-5028
    Keywords: F1F0 ATPase ; chloroplast coupling factor ; mitochondrial gene ; wheat
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    Topics: Biology
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  • 89
    ISSN: 1573-5028
    Keywords: wheat ; maize ; bean mitochondria ; tRNAPro (UGG) ; gene localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The four Sal I fragments of wheat mitochondrial DNA containing the 18S and 5S ribosomal RNA genes were screened for the presence of tRNA genes. Upon sequencing, a tRNAPro (UGG) gene was found in two of these four fragments. The localization of the corresponding gene on the maize mitochondrial genome was established. Transcriptional studies have shown that this gene is transcribed in wheat and maize mitochondria. The sequence of the corresponding tRNAPro (UGG) of bean mitochondria was determined using in vitro post-labeling techniques.
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  • 90
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis var.tritici ; rye grass ; Trichoderma hamatum ; T. harzianum ; T. koningii ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Trichoderma hamatum, T. harzianum andT. koningii were isolated from wheat and rye-grass roots from a field in Western Australia. Frequency of occurrence ofTrichoderma spp. was higher on roots subjected to washing only, for both wheat and rye-grass than the roots which were surface-sterilized with 0.6% or 1.25% NaOCl.Trichoderma spp. were recovered at a higher frequency on PDA amended with lactic acid (pH 4.5) than on PDA alone (pH 5.6) or PDA with streptomycin. In general,Trichoderma spp. were isolated at a higher frequency from roots of wheat than that of rye-grass.T. hamatum occurred at a higher frequency in rye-grass roots than in wheat, whereasT. harzianum was more common in roots of wheat than in rye-grass, especially in seedling and milky ripe stages.T. koningii was recovered at a higher frequency from roots at seedling stage of rye-grass than wheat, the reverse being true at tillering stage.T. koningii was not recovered from roots of either host in any sampling when they were surface sterilized with 1.25% NaOCl. The take-all fungus was isolated from wheat and rye-grass roots more frequently at tillering and stem extension stages than others. It was severely pathogenic to both hosts in sterilized and non-sterilized soil. Addition of lactic acid, HCl or streptomycin to PDA did not affect the growth of theTrichoderma spp. tested, but the growth was slower on Martin's medium than on other media. In generalT. harzianum andT. koningii grow faster thanT. hamatum. The growth of the three species were not different at 20 and 25°C, but at 15°c growing of all species was significantly reduced. Incorporation of lactic acid into PDA prevented the bacterial growth in all treatments. Streptomycin too reduced but to a lesser degree than lactic acid. Surface sterilization with NaOCl decreased the recovery of both bacteria and fungi. T. hamatum andT. koningii reduced the mortality of wheat and rye-grass plants inoculated with the take-all fungus in sterilized and non-sterilized soil, whereT. harzianum did not protect wheat or rye-grass from infection by the take-all fungus.
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  • 91
    ISSN: 1573-5036
    Keywords: peas ; plant genetics ; plant growth ; Pseudomonas ; rhizosphere bacteria ; Serratia ; symptoms ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat (Triticum aestivum L.) and pea (Pisum spp.) genotypes were tested for reaction to root inoculation with rhizosphere bacteria affecting plant growth. Plant response was studied in greenhouse experiments after treatment of seedlings with bacteria suspended in nutrient broth. Significant genotype variation was found in both wheat and pea in terms of shoot dry weight and severity of bacteria-induced leaf symptoms. For most bacterial isolates tested, there was good correlation between ratings of leaf symptoms 7 to 14 days after inoculation and growth inhibition measured after four weeks. Interactions between isolates and plant genotypes were significant in both wheat and pea (P=0.0024 and 0.0001, respectively), but genotypes with sensitivity or tolerance to most isolates could be distinguished. In an outdoor pot experiment, two of the bacterial isolates caused delayed plant development and differential decreases in grain yield of wheat genotypes. The hypothesis that the reaction of wheat genotypes to the tested becteria was related to their influence on bacterial establishment in the rhizosphere could not be substantiated.
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  • 92
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    Plant and soil 111 (1988), S. 25-35 
    ISSN: 1573-5036
    Keywords: carbohydrate accumulation ; chloride ; salinity-phosphorus interaction ; sodium ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Foliar inorganic ion and carbohydrate concentrations were determined in wheat plants treated with factorial combinations of phosphorus fertilizer and NaCl in a glasshouse experiment. Growth reductions and visual symptoms of salt toxicity were minimized when phosphorus nutrition was adequate, and were intensified by phosphorus deficiency. Foliar sodium and chloride accumulated up to 4.0–5.5% d.w. with salinity treatment. However, ionic concentrations within corresponding leaves or distributions between leaves of plants with different phosphorus treatments were not influenced by phosphorus treatment and had no relationship to the severity of salt toxicity symptoms. This suggests that phosphorus deficiency reduced the cellular tolerance for ion accumulation. A combination of phosphorus deficiency and salinity induced an accumulation of foliar starch and sucrose despite substantial reductions in net CO2 assimilation rates. This accumulation did not occur if phosphorus nutrition was adequate, which is consistent with the roles of phosphorus in carbohydrate metabolism. It is proposed that adequate phosphorus nutrition is essential for effective ion compartmentation by contributing to efficient carbohydrate utilization in salt-stressed wheat.
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  • 93
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    Plant and soil 110 (1988), S. 140-142 
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; roots ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A root assessment tray was designed for the meticulous assessment of take-all on wheat seedling roots from soil bioassays. Subsequently, the detection of lateral root infections (in addition to the more obvious infections on main axes of seminal roots) resulted in increased estimates of propagule numbers of the take-all fungus (Gaeumannomyces graminis var.tritici) for 196 of the 368 soil samples bioassayed in a field study conducted in Western Australia between 1984 and 1986.
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  • 94
    ISSN: 1573-5036
    Keywords: barley ; boron toxicity ; genotypic variation ; mineral nutrition ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mechanism of resistance toB toxicity in barley and wheat was studied in a solution culture experiment using several cultivars displaying a large range of sensitivity to excessB supply. Plants were cultured for 35 d atB concentrations ranging from normal to excessive (15 to 5000 μM, respectively) then examined for dry matter production and theB distribution between roots and shoots. In both species, increasedB supply was accompanied by increased tissueB concentrations, development ofB toxicity symptoms and depressed growth. At each level ofB supply, however, resistant cultivars accumulated considerably lessB than did sensitive cultivars, in both roots and shoots. Even at the lowestB supply, at which noB toxicity symptoms developed and growth was not affected, resistant cultivars maintained relatively low tissueB concentrations. No cultivar displayed an ability to tolerate high tissueB concentrations. These results indicate that sensitivity toB toxicity in barley and wheat is governed by the ability of cultivars to excludeB. If theB concentrations of tissues is used to indicate resistance toB toxicity, then cultivars have the same ranking whether cultured at a normal or excessB supply.
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  • 95
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    Plant and soil 113 (1989), S. 213-221 
    ISSN: 1573-5036
    Keywords: microbial biomass ; 32P ; rhizosphere ; soil organic-P ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract 32P-labelled monocalcium phosphate solution was supplied by point injection to the root system of wheat plants grown in soil cores in a controlled environment. There was no detectable incorporation of32P into organic P fractions in the soil remaining after roots were removed, confirming field observations. The techniques used to measure organic P (including biomass P) could detect an incorporation of32P into soil microbial biomass equivalent to 0.3 μgP.g−1 soil, compared to a total soil biomass P content estimated to be ca. 6.5 μgP.g−1 soil. The limited incorporation of the added P into microbial biomass in the root-free soil may be due partly to a limited diffusion of32P into the non-rhizosphere soil and partly to the removal of32P-labelled microbial biomass adhering to or in very close association with the root surface. it is proposed that in studies of soil nutrient status, total soil biomass P (roots + soil flora + microfauna) should be measured, rather than attempting an estimate of microbial P. A sequential extraction procedure using a single soil sample, where a biocide is added to the extracting solution, is proposed as an alternative to the conventional procedure for measuring soil biomass P where two soil samples, one treated with a biocide, are extracted simultaneously.
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  • 96
    ISSN: 1573-5036
    Keywords: characterization ; deleterious rhizobacteria ; inhibitory bacteria ; pseudomonads ; toxin ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A toxin produced by a deleterious rhizobacterial pseudomonad that inhibits both winter wheat (Triticum aestivum L.) root andEscherichia coli growth was characterized. The toxin was rapidly deactivated at pH 2 and 12 and by autoclaving (121°C, 15 minutes). Less toxin was destroyed as the temperature and time of exposure decreased, and at 40°C it was stable for at least 24 hours. The toxin was extremely polar and could not be extracted from culture filtrates with organic solvents. The compound eluted after the void volume from a Sephadex G-10 column indicating a molecular weight of less than 700. The toxin adsorbed to Dowex 50W strong cation exchange resin and eluted with 2M NH4OH. Numerous thin layer chromatography solvent systems were unsuccessful at purifying the toxin. The partially purified toxin inhibited several different microorganisms while the producing strains were resistant. The toxin appears unique to toxins produced by recognized plant pathogenic bacteria.
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  • 97
    Electronic Resource
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    Plant and soil 116 (1989), S. 29-36 
    ISSN: 1573-5036
    Keywords: cytokinins ; potassium ; seaweed concentrate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of the seaweed concentrate “Kelpak’ on the growth and yield of wheat grown under conditions of varying K supply were investigated. Kelpak had no significant effect on the yield of wheat receiving an adequate K supply, but significantly increased the yield of K stressed plants. The increase in yield was caused by an increase in both grain number and individual grain weight. Although the beneficial effects of seaweed concentrates have often been attributed to their cytokinin content, several lines of evidence suggested that this group of plant growth regulators may not be solely responsible for the observed effects of Kelpak on wheat. Irrespective of the physiological mechanism of action, Kelpak would appear to have considerable potential for increasing yield in K stressed wheat and may therefore reduce the requirement of wheat for K fertilization.
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  • 98
    Electronic Resource
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    Plant and soil 117 (1989), S. 157-165 
    ISSN: 1573-5036
    Keywords: bacterial metabolites ; gnotobiotics ; growth inhibition ; plant genotypes ; Pseudomonas ; rhizosphere ; Serratia ; symptoms ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With the aim of elucidating mechanisms behind bacteria-induced deleterious effects and differential cultivar responses to bacterial inoculations, wheat seedlings were subjected to various tests under gnotobiotic conditions. Inoculation with two deleterious Pseudomonas isolates, Å 112 (fluorescent) and Å 313 (nonfluorescent), induced leaf symptoms and shoot and root growth inhibition, while inoculation with growthneutral bacteria (Serratia liquefaciens andEscherichia coli) had no such effects. Deleterious effects were induced at low inoculum densities (〈103 cells per plant), but required addition of nutrient broth in small amounts for consistency. Effects similar to those obtained with living inoculum could be induced by treating plants with sterile culture filtrates from isolate Å 313 or volatile bacterial metabolites from isolate Å 112. Wheat cultivars previously found to differ in their reaction to inoculation under non-sterile conditions, responded differentially to Å 112 and Å 313 also in the gnotobiotic assay. The results agree with the hypothesis that neither cultivar reaction nor the bacterial effects as such are mediated by interactions with an indigenous rhizosphere microflora.
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  • 99
    ISSN: 1573-5036
    Keywords: black gram ; boron deficiency ; borax rates ; green gram ; hollow heart ; hot-water-soluble boron ; kernel boron ; leaf boron ; peanut ; rice ; soybean ; sunflower ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of boron (B) on peanut and soybean was examined in two omission and one B fertilizer rate trial on a Typic Tropaqualf in Northern Thailand. The B rate trial was combined with a comparison of the response of sunflower, green gram, black gram, wheat, and rice in addition to peanut and soybean grown in irrigated rice-based cropping sequences over two years. Omitting B induced the hollow heart symptom in 10% of peanut kernels with the incidence of hollow hearts closely related to B concentration in the kernels. Omission of B had no effect on the appearance of soybean seed or on the grain yield of either soybean or peanut. In the B rate experiment, omitting B depressed grain yield by 50% in sunflower and by 40% to 80% in black gram, induced B deficiency symptoms in green gram and the hollow heart symptom in peanut kernels, but had not significant effect on the grain yield of soybean, peanuts, rice, or wheat. B deficiency apparently depressed grain yield in black and green gram by delaying or inhibiting reproductive development thus reducing pod set.
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  • 100
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    Plant molecular biology 10 (1988), S. 251-262 
    ISSN: 1573-5028
    Keywords: mitochondria ; mitochondrial DNA ; transfer RNA ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have begun a systematic search for potential tRNA genes in wheat mtDNA, and present here the sequences of regions of the wheat mitochondrial genome that encode genes for tRNAAsp (anticodon GUC), tRNAPro (UGG), tRNATyr (GUA), and two tRNAsSer (UGA and GCU). These genes are all solitary, not immediately adjacent to other tRNA or known protein coding genes. Each of the encoded tRNAs can assume a secondary structure that conforms to the standard cloverleaf model, and that displays none of the structural aberrations peculiar to some of the corresponding mitochondrial tRNAs from other eukaryotes. The wheat mitochondrial tRNA sequences are, on average, substantially more similar to their eubacterial and chloroplast counterparts than to their homologues in fungal and animal mitochondria. However, an analysis of regions ∼ 150 nucleotides upstream and ∼ 100 nucleotides downstream of the tRNA coding regions has revealed no obvious conserved sequences that resemble the promoter and terminator motifs that regulate the expression of eubacterial and some chloroplast tRNA genes. When restriction digests of wheat mtDNA are probed with 32P-labelled wheat mitochondrial tRNAs, 〈20 hybridizing bands are detected, whether enzymes with 4 bp or 6 bp recognition sites are used. This suggests that the wheat mitochondrial genome, despite its large size, may carry a relatively small number of tRNA genes.
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