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  • Feeding  (48)
  • Chemistry
  • Iranian Fisheries Science Research Institute  (50)
  • 2015-2019  (50)
  • 2005-2009
  • 1985-1989
  • 1
    Publication Date: 2021-05-19
    Description: Larvae of the chironomid midges (blood worms) are known as use full live food for cultured aquatics. There are endemic blood worms in sediments of the earthponds of Bafgh area at center of Iran. This study tended to identification of two chironomid species, Chironomus aprilinus Meigen, 1830 and Dicrotendipes sudanicus Freeman, 1959 & 1961, from Iran as a new record. Culture of blood worms was successful in glass thanks with 16h. light, at 24-26°C temperature. Eggs were incubated after 14 days. Suitable concentration of harvesting is 1-2 egg masses (1250eggs/m2). Enrichment of earthponds or plastis thanks including sedime is offered for culture of chironomid larvae. Collectors prepared with low cost materials are useful for collection of egg masses. May, June and July are the best time for egg collection. Large blood worms are mainly avalable in winter. Results of body analysis of the larvae indicated high dry matter with good quality.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biotechnic ; Chironomid ; Identification ; Culture ; Collector ; Brackish water ; Chironomidae ; Aquatic ; Larvae ; Blood worm ; Sediments ; Species ; Chironomus aprilinus ; Chironomus aprilinus ; Feed ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 2
    Publication Date: 2021-05-19
    Description: Use of underground inland water for fisheries aims is improved all over the world. Tilapia is the first candidate for brackish water fish culture and an economic fish based on its biological characteristics. Tilapia was entered to Iran for the first time in November 2008. Bafq area at the center of Iran was licensed by Environment Organization, because of closed underground water resources of the area. Studies showed desired growth and adaptation of Nile tilapia in brackish water earth ponds and fiber glass tanks with high survival and low FCR. Limnological factors were suitable for tilapia culture. All male samples were made using certain doses of methyl testosterone in defined period. Optimum level of protein, fat and energy were determined for the best growth and food performance of cultured tilapia. Environmental program was presented to prevent environmental problems. However, studied shoed tilapia culture had not negative effects in the area. Economical performance of tilapia culture project was desirable. Tilapia was successfully introduced to some private fish farms in June 2011. Tilapia culture may improve in center of Iran after environmental surveys.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Tilapia ; Aquaculture ; Monosex ; Feeding ; Environment
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 76pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Menmiopsis leidyi was entered into Caspian Sea from Black Sea and mass population constitutes in the southern Caspian Sea. In this surveys, the relationship between length and wet weight measured and length and weight followed by this equation: n= 384, R^ 2=0.87 w= 0.003 L^ 9005 . The relationship of dry and wet weight calculated, in this surveys, the ratio of dry weight to wet weight was equal to 1.615% in 12 salinity. In vitro, the clearance rate at 12 and 27 C were 52.48 and 107.33 ml/h, respectively, daily ration at above temperatures were 1.98 and 16.65 respectively. Mnemiopsis leidyi can feed Acartia in accordance to 16.5 mg/1gbw (body weight) at 27 C in one day. The average digestive time in 12 C and 27 C were 77.50 min and 36 min respectively. The digestive value in 27 C was 2.5 fold than 12 C . The result indicated that clearance rate, daily ration and digest were increased in high temperature. Stomach content of Mnemiopsis leidyi was shown that this organism utilized from Acartia and Balanus, in addition, stomach content of kilka including two species of zooplankton from the southern Caspian Sea. Stomach contents of clupeonella cultiventris contain more than 54 percent of Acartia and its nauplii. This frequency observed in cluponella engrauliformis and clupeonella grimmi. On basis of zooplankton sampling in the sea, over 90 percentage of zooplankton populations contain Acartia.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Menmiopsis leidyi ; Zooplankton ; Population ; Survey ; Feeding ; Cetenophora
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 45pp.
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  • 4
    Publication Date: 2021-05-19
    Description: Blackmouth croackers (Atrobucca nibe) resources are the most important commercial stocks in the north-west of Oman Sea, exploited as lantern fisheries by catch with trawlers vessel. At least 50 specimens were sampled monthly from September 2012 to July 2014. Results showed blackmouth croacker have several small peak in gonado-somatic index, but the most frequency of adults occurred in May. The female: male ratio was calculated 1:0.8 and the LM50 was estimated at 35 cm. The mean of absolute and relative fecundity were estimated 92988 and 223 respectively. LM50 and LC50 were calculated and the results showed this species mainly caught before length of maturity, and the mass populations have not sufficient opportunity to reproduction. The mean of egg diameter for black mouth croaker were calculated at 533±103. Linear model of Fecundity= 9597.6TL-233880 was calculated for length-fecundity relationship . Results of feeding showed lantern fishes (65%), shrimps (11%), Japanese threadfin bream (13%) and the other fishes (11%) were the main feeding regime of black mouth croaker. This species was carnivore, the food prefect mainly consisted of lantern fishes (57.7%) and shrimps (28.2%). The vacuity index (87.7 %) indicated this spices was low appetite fish. Total length range was 18 to 47.5 cm with mean length of 28.7 cm, and the class of 23-24 cm was the most frequent length in fishing ground. The length-weight relationships in A. nibe were calculated for female and male W= 0.0121L2.9385 and W= 0.0075L3.0737 respectively, both have isometric growth. Growth parameters of L∞ and K were estimated 50 cm and 0.2 y-1 respectively. Parameters of t0, Tmax and Ǿ were calculated -0.7 year, 14 year and 2.69 respectively. This species have two recruitment period, and the peak of recruitment was occurred in May with 23.14 percent. Total mortality , natural mortality and fishing mortality rates were calculated 0.53 y-1, 0.46 y-1 and 0.07 y-1 respectively. Due to the calculated exploitation rate (0.13), it is appreciated the improvement of black-mouth croaker in the Oman Sea. Base on seasonal and monthly CPUE it was resulted the peak of fishing density were occurred in the winter. Results showed the main distribution of black-mouth croaker concentrated on the continental slope in 200 m depth, but these stocks move to deeper waters ( more than 200m) in warm season and extend to shallow waters (less than 200m) in winter.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Atrobucca nibe ; Reproduction ; Feeding ; Growth Parameters ; Mortality rates ; Exploitation rate ; Distribution ; Survey ; Black mouth Croaker
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 87pp.
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  • 5
    Publication Date: 2021-05-19
    Description: This investigation conducted for evaluation Silo (Cylindrical) Fish Tanks as an applicable method for aquaculture of Rainbow trout (Oncorhynchus mykiss). Current research was designed in two practical phases as accidental experiments through 3 treatments and repeats of which stocked by three Rainbow trout (375 gr) densities as 75, 100 and 125 fish/m3 in 9 silo fish tanks (2 m3). For better understandings of the silo performance method, concrete circular fish tanks (similar volume) considered as controls and stocked as similar fish densities as silo tanks. The duration of the first phase of experiment was 150 days. The second phase of the experiment was determined for evaluating two proposed silo type (Inlet and outlet in bottom side / Inlet from bottom and outlet in upper side). In this stage, proposed silos (stocked by fish of 294 gr weigh) tested for rearing trout for 30 days. Water supplied from two surface wells after some aeration operations. Stocked fish were fed by pellets made by Fish Feed Chineh Company (Ltd) in Iran. Feeding operation conducted according to the recognized manuals by consideration of water temperature, fish weight and biomass. Meanwhile, biometry operation conducted in 10 days intervals while fish were anaesthetized. In addition, water quality was determined by daily measurements. According to the results of the first phase of experiment, there were significant variations on Growth rate, survival rate, FCR, FCE and SGR through the cultured organisms in silo tanks compared to the circular tanks. Meanwhile, according the results of an economic study on this survey, the fish culture in silo tanks showed significant economical and profitable in compare to controls. In additions, the second phase of experiment showed better aeration conditions in silo tanks when inlet water supply from bottom side and outlet located in upper side. Because of the fine distribution of fish in silo tanks and more usage of depth by this method, fish culture would be an applicable and profitable technique in narrow lands and slopes through the mountains area. The maximum stocking rate recorded more than 100 Kg fish/m3 in silo tanks.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Practical ; Silo ; Cylindrical ; Aquaculture ; Rainbow trout ; Oncorhynchus mykiss ; Fish ; Feeding ; Temperature ; Weight ; Biomass ; FCR ; FCE ; SGR ; Culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 73pp.
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  • 6
    Publication Date: 2021-05-19
    Description: In the first chapter, all documents in aquaculture feed, feed formulation, nutritional requirement and basic concepts of aquatic feeding had been collected. The second chapter focused on broodstock feeding in order to improve reproduction effecieny . The third chapter performed to importance of live food specially in sturgeon fishes larvae and the forth chapter attended to biology and ecology of this fishes and finally in the fifth chaper, special nutrition and feeding in sturgeon fish larvae had been brought with all the works out in Iran on. There are 63 documents including papers, thesis and reports from different research library and universities among them 34 base on sturgeon larvae nutrition specially on Huso huso and Persian sturgeon species and almost there are not any documents on the other three species. 14 documents comes from the role of protein, energy and lipid replacements basically in the first growth year, 3 of back to the special nutrition requirement of Persian and Huso huso species and 12 were on digestive enzymes physiology, the role of probiotic and prebiotic on growth and survival rate. Almost there is not any organization in Iran to work on this species specificly and it seems every student and researcher paid attend to a narrow way of high way sturgeon fish researches. There is not any data bank on these important fishes and some repeated worked out by researchers in different parts of the country. Beside of this documents there had been gathered some research documents from the other countires for comparing and showing the correct way in this regards. First, we must focus on broodstock sturgeon feeding, then on egg and yolk analysis, larval stages, morphology, measurement of larval mouth and finding the best live food for different stages of sturgeon larviculture and finnaly on digestive physiology and enzymatic activities. In the stage of shifting from endogenous to exogenous feeding, nutritional requirement, protein digestibility, essential and free amino acids, essential fatty acids spcially PUFA and HUFA, vitamins, minerals, pigments, growth and survival stimulants and resistance to physiological environmental stress are very important to search.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sturgeon fishes ; Nutrition ; Feeding ; Biological stages ; Feed ; Larvae
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 256pp.
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  • 7
    Publication Date: 2021-05-19
    Description: The intensive culture of common carp (Cyprinus carpio) evaluated in fiberglass tanks(2*2m) with feeding on pellet during 8 weeks.The fish (n=201)of common carp selected from three treatments along with three replicates as the average of fish weight was less than 100(g )which divided into 〈50(g), 50-70(g) and 70-100(g).At the first experiment , there is no statistically significant difference between fish weight in relative to replicate treatment (P〉0.05).The common pellet food was chosen for feeding from market as fish were fed by the mean of 4% of their body weight a day. The result indicated that the mean of particular growth ratio at first treatment (0.54) has higher than second treatment (0.34) but in comparison with third treatment (0.46), there was no statistically significant difference (P〈0.05).Also, the conversion ratio at first treatment (5.28) and third treatment (6.09) was lower than second treatment (35.66) and third treatment (20.91) was lower than second treatment (24.24), (P〈0.05). This study showed that monoculture in small scale is eassible by utilizing artificial diet, if the water quality is exposed to change. In this survey, the conversion ratio of the fish was relatively high whereas the growth rate was low.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Common carp ; Mono culture ; Pellet ; Fiber glass tank ; Cyprinus carpio ; Feeding ; Weight ; Growth Rate ; Artificial diet
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 21pp.
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  • 8
    Publication Date: 2021-05-19
    Description: Taking into consideration culture of beluga (Huso huso) in new condition such as inland brackish water, it is very important to study nutrition requirements and effect of different diets on gonadic and somatic growth as well as physiological condition. In this research 74 beluga (4 years old) cultured in brackish water earth ponds in bafgh fisheries research station, were selected and distributed in 8 circular cement ponds. Feeding was done in 4 formulated diets with fixed protein level and 4 energy levels (400,425,450,475 kcal/100gr). Samples were biopsied in the first and the end of experiment to determine sexuality and stage of maturation. To study gonad tissues, the hematoxylin-eosin method was used. Results indicated that somatic and gonadic growth index was affected by diets. Growth somatic overlapped in both sexes. Sexual maturation stages were not the same in both males and females and transition from stage II in males was more rapid than females. Considering the results in this study, diet treatments influenced on somatic growth in both sexes and gonadic growth in females. It seems to state of somatic and especially gonadic growth in brackish water is suitable. Therefore, inland brackish water environment can be introduced as a suitable environment cultivation of beluga.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physiological ; Beluga ; Huso Huso ; Brackish Water ; Protein And Energy Requirements ; Gonadic Growth ; Somatic Growth ; Nutrition ; Feeding ; Sample ; Sexuality ; Tissue
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 95pp.
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: The culture of Iranian Shrimps began in Bushehr in 1372. Early the green or the pink tiger shrimp (Penaeus semisulcatus) which were found in most habitats of the Persian Gulf and Oman Sea were considered in breeding and reproduction, but due to reproduction difficulties, it was rather replaced by the Monodun imported species, then the production of the white shrimp Metapenaeus affinis and Then P.merguensis in some southern areas of the country such as Hormozgan has began in small scale,but none of these species couldn't meet the economical needs of the consumer society and they were not indelible and dominant enough in the shrimp aquaculture industry of the country until breeding of Indian White Shrimp (Fenneropenaeus indicus) has began in large scale in the provinces of Khuzestan and Bushehr, which are seen as the heart of shrimps reproduction and breeding, and in Hormozgan and Sistan. the incidence of white spot viral syndrome (White Spot Syndrom Virus) led to the import of the western white leg shrimp from the USA (P.vannameii) by the Iranian Fisheries Research Organization (IFRO) in 1383, at the present all southern and northern breeding farms of the country (fundamental measures has been carried out in line of shrimp reproduction in Gorgan province in 1386) has put the breeding of this shrimp species as the single breeding species at top of their agenda. The subject of feeding the breeding shrimps is widespread and regarding to the non-proprietary species there are still some species which contain high protein than the other species in studying the different shrimp species requirements. In Iran due to the scarce factories producing the shrimp's food, the alimentary compound of the breeding shrimps during industrializing years of this type of breeding even after the non-aboriginal western white leg shrimp species being inclusive was stable and through different biological processes including: Naplies which feeds from its yalk and by entry into zoa stage start eating only small-sized phytoplankton. And by entry into the stage between zoa and mysis it feeds from phyto and zooplankton simultaneously and entering the post-larva stage it stars sarcophagi. and after 15 post-larva stage entering the growth and transition stage to earthen ponds feeding by concentrated industrial foods in large and small packages depending on young and adult mouth and due to feeding requirements of every stage the alimentary compound is nearly as follows: Digestible protein, energy, fat, carbohydrates, vitamins and minerals will start and the final product will be released in market Or they will be used in the later years of laying eggs and feeding for brood stock. In feeding section the details of every stage and their food's nutritional needs at each stage and will be fully described.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Economical ; Shrimp ; Food components ; Biological processes ; Nutrition ; Feeding ; Culture ; Tiger shrimp ; Penaeus Semisulcatus ; Breeding ; White shrimp ; Metapenaeus affinis ; Aquaculture ; White Spot Syndrome Virus ; Phytoplankton ; Zooplankton
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 178pp.
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  • 10
    Publication Date: 2021-05-19
    Description: Acipenser persicus, one of the native Species living in the southern part of the Caspian Sea, has high Percentage of larval mortality. Therefor to decrease the rate of mortality of larvae survival and to increase to regenerate properly during the active feeding process of fish larvae, while under artificial reproduction they were fed withe fresh water rotifer. Four types of feeding treatments were applied as follow :Treatment 1: Similar to normal process , first withe Artemia cyst then withe Daphnia Treatment 2: Mixture of Artemia, Rotifer and Daphnia; Treatment 3 freshwater Rotifera ;Treatment 4: fresh water Rotifer enriched with Vitamin C (Ascorbic acid- 6- Palmytat ).Three replicates were used for each treatment. In the process of each observation 45 larvae in containers withe 100 liter capacity filled with 30 liters of water , after 8 days of external feeding process, were inspected closely, during which , they were under biometric measurement once every two days. Larvae were fed Four times a day at a rate of 25 % of their body weight. The average temperature estimation was 22/5 ±0/5 centigrade, pH of water being 8/5 ± 0/1 while Oxygen proved to be 9/58 ± 0/2 mg/l . At the end of the observation, the profill fatty acids of the remains were studied .The growth factors, the mouth size of the larvae and the expansion of the mouth size compared to the total length of the larvae during the whole observation were inspected. Credibility of data designated was examined by Shapiro Wilks test. Specific growth rate (SGR), weight growth (WG) and feed conversion ratio (FCR) were contemplated by one- way analysis test, and significant difference Tukey's test. The most notable diversity was evident between treatment 2 , whose indicative factors were in the following order : 4/ 65 ± 0/06, 45/18 ± 0/66, 4/48 ± 0/07 and treatment 4 whose indicative factors showed the following results: 10/47 ± 0/04, 124/42 ± 0/62, 1/51 ± 0/008.For the determination of fatness rate and weight gains, Kruskal Valis and Man vit ni test were used .In this part of study , the highest diversity fatness appeared to be between treatment 4 ,reflecting 0/79±0/07 and treatment 2 showing the result 0/62±0/05.The highest gain was related to treatment 4, whit the rate of 99/33 ± 0/68 mgr and the lowest gain occurred in treatment 2 white the rate 62/36 ± 0/65 mgr . This study also revealed that, the survival ratio connected with treatments 3 and 4 , compared treatments 1 and 2, were significantly different. The profile of fatty acids also showed much higher percentage of PUFA and HUFA in treatments 3 and 4 , than those in other treatments. Furthermore Brachionus calyciflorus proved to be a proper food supply for larvae. Therefore by building suitable pools next to the work side, other kinds of species fresh water rotifer could also be used as food improvements for feeding larvae.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Brachionus calyciflorus ; Acipenser persicus ; Fatty acid ; Larvae culture ; Vitamin C ; Larval survival ; Species ; Mortality ; Feeding ; Artemia ; Cyst ; Ascorbic acid ; Oxygen ; Growth ; SGR ; FCR ; Fresh water
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 11
    Publication Date: 2021-05-19
    Description: This project was carried out in order to determine the hatching conditions and laboratory culture methods of Fairy Shrimps ( Phallocryptus spinosa). Physico- chemical factors and phytoplankton of natural habitat of this organism was investigated in Khasellou region around Azarshahr – East Azarbaijan province. The results showed that occurance and growth of fairy shrimps in their habitates were beginning from early Aprill and their survival was decreased with increasing temperature, precipitation and increasing the salinity of their culture medium. Then they disappeared. Their habitat phytoplankton contained 3 phylla and 10 genera, from which green algae enjoyed the greatest density in May. The pool salinity was around 20-17 g/l , when metanauplii was observed in early spring. In the pool containing and without fairy shrimps the oxygen concenteration was high (9 mg/l) and low (2.9 mg/l ) , respectively . In 9 prawn ornamental fish genera, feeding with fairy shrimp showed a significant higher fecundity and hatching percentage compaired to manual diets (cow liver, spinach). In all groups fed with fairy shrimp the duration of spawning time were reduced from 15 to 8-10 days and the color was enhanced. The means of cyst number per captured female also, cyst , decapsulated cyst and Naupilli diameters were mesured 142.9±19.0 cysts , 273.2 ± 4.9 µ , 242.4± 3.8 µ and 542.6 ± 27.0 µ., respectively . The hatching rate was increased by reducing temperature and salinity of their culture mediums from 25 to 15 ◦ C , and 28 to 18 g/l, respectively.The highest hatching was observed on 5 th day at 15 ◦ C and 18 g/l (5, 33 %). In addition, low but multiple hatching was observed. Therefore , water temperature and salinity affected hatching rate in this species.The hatching success of Phallocryptus spinosa cysts was beter when collecting cysts together with dry sediments of their habitat without separation sensetive cysts . In addition to cold keeping and freezing, the hydration – dehydration method caused more diapause deactivation for hatching induction. The laboratory results revealed that the survival of fairy shrimps were highest at 15 ◦ C (42%) and lowest at 25◦ C (26%), respectively. Also, their life was inhibited in 30◦ C.In different salinities, the mean survival rate were 26% (Salinity = 15 g/l) and 20% (Salinity = 25 g/l), respectively. The mean survival percentage of Phallocryptus spinosa cultured in natural habitat water (64.3%) was higher than aerated tap water (23, 3 %). Also, feeding with Nanochlorepsis caused higher survival rates. On the other hand, Beakers yeast caused higher mortality rates. Therefore , regarding to relatively high sensitivity of these species to physico – chemical and nutritional conditions of their culture medium, it is better to culture these species with expantion of their natural habitat in order to preserve their biodiversity , culture and increase stocking density with regard to environmental considerations .
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physico-chemical ; Environmental ; Fairy Shrimps ; Phallocryptus spinosa ; Hatching ; Culture ; Biotechnique ; Species ; Anostraca ; Phytoplankton ; Growth ; Temperature ; Salinity ; Phylla ; Feeding ; Survival rate ; Mortality ; Biodiversity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 104pp.
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  • 12
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: For hand feeding of Grass carp (Ctenopharyngodon idella), a kind of fish used in integrated carp culture, two kinds of pellets with Alfa alfa as current food were suggested as follows: 1. Pellet B with low fiber and high protein 2. Pellet C with high fiber and low protein The ingredient ratio was powdered and mixed, by adding water, fiber was made and minced the minced pellet was put on net tray and then in blotter to be dried and ready for use. In this experiment food quality analysis and also food water stability was measured. Regarding sampling, growth rate and weight increase of fish and FCR of each ratio was determined. Results indicates diet C is not able to compete with Alfa alfa from the stand point of weight increase, FCR and price. But diet B with low FCR and higher growth rate and higher price (not meaning ful P 5%) can compete with Alfa alfa.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Grass carp ; Feeding ; Pellet ; Ctenopharygodon idella ; Fish ; Culture ; Alfa alfa ; Fiber ; Protein ; Sampling ; Growth ; FCR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 33pp.
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  • 13
    Publication Date: 2021-05-19
    Description: Survey is conducted to evaluate the effects of different intensities and durations of red light spectrums on growth performance and survival of Caspian trout (Salmo trutta caspius) in Coldwater Fishes Research Center (CFRC). According to the standard methods and manuals, selected 1.1±0.2 g (1755 individuals) larvae transferred from a registered and well-known hatchery and introduced to the fish tanks in CFRC after quarantine and adaptation procedures. The trial designed for 9 treatments with 3 repeats and fish tanks (California troughs) stocked by 65 larvae. All tanks were separated by dark plastic sheets individually to more controlling actions on special lighting regimes, except controls. Lighting actions were conducted as long as survey stage for 36 days by economic lamps (P: 40/50 W, =550/880 lum) on lighting durations of 12/12, 10/14, 14/10 h (on/off) and 24 (on) by 40/50 W lamps respectively for treatment 1 to 8. Controls are considered in treatment No. 9 by natural and environmental lighting regime. The biometry operations were carried out in a 6 days interval. Results were analyzed with SPSS (Version 12) software and by ANOVA one way analysis method and multiple levels of Duncan Test; graphs were illustrated by Excel software. According to the results, most of the measured factors as growth indicators (FW, WG, FCR and SGR) in treatments (1-8) had showed significant differences in compare of control. In this case, oily drips in retina layer of eye s larvae absorb the short light spectrums and let the long spectrums to be transferred. Thus because of more vision, feeding behavior could develop with lower stress condition. Moreover, some growth factor (SGR, WG and FCR) rates in larvae showed better values significantly in treatment (1) (12/12 h lighting with 550 lum) in compare of other treatments and controls (P≤ 0.05). In addition, there are not any harmonized and expected growth parameters with synchronized red lighting intensities and durations in the Caspian trout larvae. It seems that recent demonstrations were influenced by physiological effects of some endocrinal glands and brain neurotransmitters.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physiological ; Environmental ; Caspian trout ; Salmo trutta caspius ; Larvae ; Red light ; Light strengthen ; Light duration ; Growth ; Survey ; Survival ; Coldwater ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 54pp.
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  • 14
    Publication Date: 2021-05-19
    Description: The study on economic aspects of tilapia culture in Iran is a subproject of a comprehensive project on introduction of tilapia to central regions of the country. Two types of red and black of the exotic tilapia were imported from Indonesia in 2009 for research and were transferred to the research center of Bafgh. Since tilapia has capabilities in aquaculture such as resistance to diseases, fast growth, tolerance to high intensity, growth in freshwater, brackish water and in saline water, also ability to grow in pond, cage and aquaculture tanks, so it is likely that it’s production be of economic advantages. Therefore the present study looked at economic aspects of raising tilapia. The factors examined were feed expenditures, wages, fertilizer spending, costs of fish fingerlings, and pond preparation in two treatments of black and red tilapia in 0.45 hectare earth ponds, 3m2 aquaculture tanks with two replicates. Results of data analyses showed that benefit of each pond for black tilapia would be about 5968763 Rials. For red tilapia this amounted to 5939696 Rials. On total the benefit for black tilapia pond culture was 11940444 Rials while this for red tilapia was 11879392 Rials, so we can conclude that pond culture of tilapia is beneficial. Culture cost of black tilapia in tanks with 57 Kg outcome, was 2540600 Rials. Accordingly the cost of each kilogram of fish is 44572 Rials, so with 20 percent benefit, the price for each kilogram is 53485 Rials. Also for producing 37 kilograms red tilapia in tank some 2531500 Rials was expended. Each kilogram red tilapia cost 68419 Rls to produce which is much more than other cultured fish and if we expect 20 percent benefit the price of each kilogram of red tilapia would be 82103 Rls. So it is concluded that tank fish culture of tilapia would not be beneficial and economically speaking there would be no point to raise tilapia this way.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Red and Black Tilapia ; Economy ; Pond culture ; Tank culture ; Feeding ; Freshwater ; Brackishwater ; Saline water ; Tilapia ; Aquaculture ; Fertilizer ; Fingerlings
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 59pp.
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  • 15
    Publication Date: 2021-05-19
    Description: Biology aspects of cobia (Rachycentron canadum) were investigated from October 2005 to December 2006 in Northern waters of Persian Gulf (Hormozgan province).The reproduction cycle, sexual maturity, sex ratio, Fecundity, Lm50, feeding, length frequency, growth parameters and mortality of cobia were studied from total of 509 specimens ranged from 19 to 159 fork length. Gonadosomatic index peaked during spring and summer with main peak in June. Spontaneous spawning occurs around the year with peak in June. The overall female to male ratio was significantly 1 : 1.49 (P〈0.05). Batch fecundity were estimated 1684954±118990 in 15 females. Relationship between total length and fecundity were calculated F=1.3717TL 2.9567 (r2= 0.82). Feeding studies indicated that the bony fishes were main food of cobia (76%) and followed by crustaceans (25%) and mollusks (11%). Rays were least food items (22%). Maximum and Minimum GaSI were observed in March (33%) and August (0.07%) respectively. Females reached 50% sexual maturity at 81.25 cm TL. The total length -weight relationship was W=0.0042L3.1162 ( r2=0.9852). Thetotal length-fork length relationship was TL= 1.1561FL-2.533 (r2= 0.9933). Growth parameters K, L ,and t0 were calculated by von bertalanfy growth equation : 0.11 (year1), 168.65 cm, 3.49 and -0.97 respectively. The instaneous rate of total mortality (z), natural mortality (M) and fishing mortality estimated by catch curve analysis were 0.30, 0.25, 0.14 (year1). Exploitation rate and Tmax were 0.36 and 28 yearrespectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rachycentron canadum ; Cobia ; Sex ratio ; Fecundity ; Lm50 ; Feeding ; Mortality ; Growth ; Parameters ; Biology ; Maturity ; Spawning ; Fecundity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 85pp.
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  • 16
    Publication Date: 2021-05-19
    Description: In order to study phytopague fingerlings feeding in fish ponds, simultaneously to the beginning of warm season and cultivation, fingerlings of 3-5 gr from 4 ponds in Mazandaran province were captured, fixed with formalin, then transferred to the plankton determination laboratory of Caspian sea ecologic institute. Based on carried out studies on the first and the end party of their intestine and the pond water, phytophague fingerlings feeds on phytoplankton, zooplankton and detritus. But the essential nutrition was on phytoplankton and detritus. Planktonic groups were formed of Cyanophyta, Chlorophyta, Chrysophyta and Euglenophyta. But in addition to these four groups Pyrophyta was present in fish pond water. Relative abundance of these nutrient organisms in water and intestine was different. Also in most cases a direct relation was observed between the intensity of water organisms and intestine ingredients. Based on this study, essential differences of digestion were observed following to the source of nutrition and the most difference were considered in digestion of Chrysophytes, Cyanophyta and Chlorophyta. Base on the results of this study we propose to make more studies on fertilization and the proportion of different fertilizers in fish ponds in order to obtain easily digestible phytoplankton thus increase in fish exploitation.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Silver carp fry ; Nutrition ; Phytoplanktons ; Zooplanktons ; Chrysophyta ; Cyanophyta ; Clorophyta ; Pyrophyta ; Euglenophyta ; Feeding ; Hypophthalmichthys molitrix ; Fingerlings ; Fertilization
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 83pp.
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  • 17
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: In this work we have surveyed on six ponds of p.semisulcatus shrimp in the investigation farn1 that have located at helleh site in the Bushehr province from May 2001. Our main goal in this project were : (l) Measurements of quality factors in water and sediment (2) Offering possible proposals for making development in water quality of culture ponds With respect to the time of shrimp stocking and harvesting the frequencies of water and sediment sampling were twice and once per month , respectively. Also two sediment sampling of the pond bed have done before pond watering and after harvesting The measured factors in the water were : pH , salinity , dissolved oxygen , transparency , temperature ( Twice a, day in the morning and the afternoon ) , chlorophyll-a nitrate , nitrite orthophosphate , iron , biochemical oxygen demand , chemical oxygen demand , TSS , T DS, total alkalinity, organic nitrogen the sediment were : pH , alkalinity , iron , total organic matter (TOM) , total nitrogen and grain size The measurement of grain size have done in two times ( before pond watering and after harvesting The results show low concentration of the essential nutrient such as phosphate and nitrate , high concentration of ammonia , high pH , undesirable transparency , high alkalinity of sediments and a great amount of clay in pond bed On the basis of above description , we need to renewing our management in fertilizing , feedings , liming and important the quality of bed.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Survey ; Penaeus semisulcatus ; Dissolved oxygen ; Transparency ; Temperature ; Chlorophyll-a ; Alkalinity ; Fertilization ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 69pp.
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  • 18
    Publication Date: 2021-05-19
    Description: 0Genetic polymorphism by means of biochemical genetic markers using polyacrylamide gel electrophoresis system in four barbus fish species has been investigated. Species scientific name were Barbus sharpeyi ; Gunther, 1847 , Barbus grypus ; Heckel, 1843, Barbus xanthopterus ; Heckel, 1843 and Barbus esocinus ; Heckel, 1843.Sampling site and location were mainly in the rivers located in Khoozestan province such as Karoon and Karkheh rivers and the Dez dam. Different organs such as blood, muscle, kidney, eye and heart were sampled and analysed for the presence of tf, es, sod and pgm alleles. High polymorphism and presence of different alleles scored, but populations were not in the H-W equilibrium. Considring results, using current and avaiable genetic markers such as microsattelits is recommended for future works.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemistry ; Genetics ; Genetics Markers ; Distinguish ; Barbus ; Species ; Barbus sharpeyi ; Barbus xanthopterus ; Barbus esocinus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 62pp.
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  • 19
    Publication Date: 2021-05-19
    Description: Experiments were conducted to evaluate the effects of different dietary levels of protein (%30,%35,%40,%45) , fat (%8,%12,%16,%20) and total energy (3500,4000,4500,5000 kcal/kg ) on growth of fingerling Roach (Rutilus rutilus caspicus knipowitschi ) in four treatments with triplicates. The crude protein of experiment, fish averaged 2.60 gr stoking density of 25 fish/tank in a during of 57 days, the crude fat of experiment, averaged 5.30 gr stoking density of 15 fish/tank in a during of 67 days and the gross energy of experiment, averaged 5.30 gr stoking density of 25 fish/tank in a during of 57 days which reared into 100L-fiber glass tanks. At the end of each experiments, growth average parameters, such as weight gain(%), specific growth rate, food conversion ratio, condition factor and survival (%) were not significantly different among all treatments (P〉0/05) but protein efficiency ratio showed in fish fed of %30 and %35 protein at levels significantly different with another treatments (P〈0/05). In all of experimentes, significant difference was found whole-body protein, lipid contents of fish fed the different dietary protein, lipid and energy levels indicated that significant difference among of treatments. As regards the economics of feeding and effective protein utilization, the dietary containing %30, %16 fat levels and total energy 5000 kcal/kg would be suitable to growth roach fingerling.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Nutritional ; Protein ; Fat ; Energy ; Growgh ; Fish roach ; Rutilus rutilus caspicus knipowitschi ; Fingerlings ; Survival ; Lipid ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 20
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: The project carried out at Shrimp Research station of Bandargah in 2011 to determine the production parameters such as; Average body weight, production, survival and feed conversion ratio in closed- recirculating aquaculture system for shrimp culture. This project was performed in 4 m3 fiberglass tanks. 3 tanks was chosen for the control and 3 were assigned to treatments. Considered Species in this treatment was white shrimp (Litopenaeus vannamei) that with a density of 60 pieces per square meter, and was reared for 90 days. culture in control as usual and change water regularly, but the treatments did not change water during the period of culture. Water in the treatment tanks was going to turn the filter system and waste was removed by mechanical and coal filter embedded in the route, and after filtering was transferred to the wastewater tank. Wastewater Was aerated in this tank and after 24 hours was pumped out and return to treatments tanks. In the final of project, mean weight, survival rate and production for control and treatment, was respectively, 18.27 ± 2.84 and 20.70 ± 1.47 g, 73.83 ± 4.14 and 77.17± 13.66 percent, and 0.81 ± 0.14 and 0.97 ± 0.23 kg/m2. Statistical analysis results show that there are no significant differences found between treatments and control (P〉 0.05), although in all the above criteria, the relative improvement was achieved to compared of control. But the results of feed conversion ratio was 1.27 ± 0.16 for treatments that are significantly different (P 〈0.05) with controls (1.56 ± 0.23). This result showed that efficiency of the food was better in closed- recirculating shrimp culture system.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Shrimp ; Culture ; Litopenaeus vannamei ; Weight ; Survival rate ; Species ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 83pp.
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  • 21
    Publication Date: 2021-05-19
    Description: This project in partnership with Azerbaijan State Fisheries Department and the aim of increasing fish production hydrothermal (Carp fish) at unit area through providing scientific and practical method in applying air device and nutrition management, a field area of 8.5 hectares and in ponds with 3300 m2 at village Eylarzgy carried at Urmia city. After preparation of ponds, action to release fry density was 6000 ha piece. Percent including 55 percent of silver carp (as most fish), Common carp 31 percent, 8 percent Grass carp and 6 percent of Big head with average weights 67 to 92 grams respectively. For fertilizing ponds in addition to basic fertilizer, during development, organic fertilizers (cow) amount to 24 ton/ hectar (Aslary) once every two days and types of mineral fertilizers (chemical) rate of 1012.5 kg per in three days once were used.In addition to feeding carp fish with natural food, concentrates and food for feeing Grass carp fresh forage (alfalfa) was used. Oxygen supply required for ponds were provided with one to two air units (Air jet). Sampling and biometry of fish and water samples for water analysis and identification of plant zooplankton (of the family and genus) once every month until the end of the growing period was performed. Resulting data were analyzed with Excel statistical software. Results showed that the production rate in the studied ponds was 4.74 tons per hectare and production of 3.15 tons per hectare in year ago, the farm has 50.5 percent increase compared to year ago average production rate in the whole province (3.21 tons per hectare) of 47.7 percent and the average production rate in the country in 1385 (about 3.8 tons per hectare) 24.7 percent increase shows.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Increased production ; Ponds ; Feeding ; Air ; Warm water fish ; Nutrition ; Hydrothermal ; Carp fish ; Silver Carp ; Common carp ; Grass carp ; Big Head ; Fertilizers ; Sampling ; Zooplankton
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 63pp.
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  • 22
    Publication Date: 2021-05-19
    Description: In order to study feeding and reproductive biology of Southern meager (A.hololepidotus) in North west of Persian Gulf. 143 (74 males and 69 females) species were collected between October of 2008 to October of 2009 in coast of Khouzestan. Maximum and minimum length of fish were 143 cm and 81 cm respectively Maximum mean length (128.4±7.5) and Minimum mean length (104.86±12.47) were detected in October and May respectively. Maximum weight (27.5Kg) and Minimum weight (5.7Kg) and also mean weight to males (15222±1371) and Min mean weight of females (16942/02±2095) were detected. Absolute fecundity (4111847.66) and relative fecundity (268.33) were analyzed. GSI Analysis showed that these species (A.hololepidotus) spawn during June to September with highest spawning rate in September. Results showed that Southern meager is a batch spawnerspecies relationship between length and weight of male Y=0.042L^2.70(n=74,R2 =0.86) and female species Y=0.061L^2.61(n=69, R2=0.85) and also total species Y=0.059L^2.62 (n=143, R2=0.86) were measured. Due to not finding Immature species during sampling LM50 wasn’t analyzed. Analysis HSI and K index showed highest value in April and May and lowest value in August and September respectively. Prey occurrence index showed that fish is main food item and Crustacean specially Shrimp and Mollusca are secondary food item of this species. Analysis of vacuity index showed that females of Southern meager are more gluttonous than males and this species feed during spawning season .In general we can classify Southern meager as a moderate feeding rate species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biology ; Argyrosomus hololepidotus ; Feeding ; Reproductive ; Meager ; Species ; GSI ; Sampling
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 23
    Publication Date: 2021-05-19
    Description: The project of Age, growth, feeding regime and fecundity of Kutum (Rutilus frisii Kutum) survey started in south of the Caspian Sea from 2006 to 2007. The aim of project was related to determination of length maturity (Lm50%), identification of feeding animals, and investigation some of biological aspects uncluded in sex ratio, growth and age structure in southern of the Caspian Sea. Total 2706 specimens of Kutum were surveyed. The age structure of Kutum has nine age groups that included in 1, 2, 3, 4, 5, 6, 7, 8, and 9 years. The result showed that maximum frequency was about 39.3 % and were belong to 4 years and minimum frequency which was about o.1 % were belong to 9 years. The maximum and minimum fork length and weight were 58 and 21cm with mean (±SD) 38.4 (± 6.4) cm; 2450 and 104g with mean (±SD) 784.2 (± 383.30) g , respectively. There are significant different between fork length and weight in male and female of Kutum at difference months (ANOVA, P〈0.05). The sex ratio of males to female was 0.65: 1 (males: females). The least and high condition factor was 1.5 in January and 2.17 in October, respectively. Length maturity (Lm50%) was 39.07cm. Minimum and maximum absolute fecundity was varied from 15713 to 130737 eggs with mean (±SD) 69961.7 (± 3836.4). Significant different was between absolute fecundity with fork length and weight (p〈0.05). Gonadosomatic index (GSI) mean for females were 2.5±0.2 (autumn) and 17± 0.2 (spring); 1.2± 0.1 (autumn) and 4.1± 0.2 (spring) for males, respectively wich coincided to spawning period. The highest feeding intensity (Gastrosomatic index) for females was 313.9 ± 65.9 in October and 119.4 ±59.2 in March; 350.7 ±103.7 in October and 144.1±85.04 in March for males, respectively. There are significant different between GSI with male anfemale in difference months (ANOVA, p〈0.05). The Bertalanffy growth equation for all populations of Kutum was L (t) = 63.92[1-e ^0.19 (t (-1.00) ]. Main prey mature of Kutum was Cerastoderma lamarki; Gastropoda and Balanus were subordinate, and Crab, Hypanis, Fish (Neogobous sp.) Algae, egg, and scale were random preys. Planktone groups comprise to Exuviaella, Nitzchia, Osillatoria, Synedra, Nematoda, Navicolla, Diatoma, Rholcosphenia were preys juvenile of Kutum. Based on ISI (Important species index), Cerastoderma lamarki and Balanus were dominant species in feeding of Kutum. As a result, growth, fecundity and length maturity of Kutum declined which may due to loss natural breeding area, overfishing (overlapping between fishing and spawning period) and artificial breeding that commenced since 1982. Hopefully, this information can be used in fisheries management.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Age ; Growth ; Feeding ; Regime ; Reproduction ; Sexual maturity ; Rutilus frisii kutum ; Coastal waters
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 24
    Publication Date: 2021-05-19
    Description: In this study monoculture of roach (Rurilus rutilus caspicus) in freshwater and brackish water with 3 stocking densities as 10000, 15000, and 20000 specimens per hectare with 3 replications each carried out from the beginning of May till October 2001. Moreover, in order to, compare cultivation of roach in freshwater with brackish water another density of 10000 specimens per hectare with 3 replication also took place. Fingerlings from the Sijoval cultivation Center were stocked in a pond for wintering and after preparation of rearing ponds in second year were introduced to the pond according to appropriate and calculated stocking density. Fingerlings were fed by concentrated food which is given to white fish for feeding (SFK) up to 30 g in weight of fingerlings. Feeding calculation based on percentage of body weight varied from 5 to 15 % of body weight. Moreover, appetite of fish also considered for feeding the fingerlings. Average weight and length in the first year from hatching time up to the end of rearing period were 5.55±1.58 g and 8.57±1.02 cm. Results indicate that in the second year and in the different stocking density the average weight and length at the end of cultivation period with minimum treatment were 56.68±16.5 g and 16.79±1.21 cm respectively. In maximum stocking density the mean average length was 16.74±1.23 cm and mean average weight was 55.33±16.5. In order to compare cultivation of roach in freshwater with brackish water another stocking density of 10000 specimens per hectare with 3 replications also took place in this study. Fingerlings with average weight of 6.5±1.82 g and average length of 9.5±1.24 cm were introduced to the ponds. At the end of rearing period average final weight was 48.53±9.41 g with average length of 15.15±1.08 cm. Average daily growth in freshwater treatment from the minimum to the maximum stocking density was 0.22, 0.26, and 0.23 respectively. In brackish water replication the average daily growth was 0.22. FCR in the freshwater treatment from the minimum to the maximum stocking density was 4.4, 4.46, and 4.4 respectively and in brackish water it was 3.14. Average condition factor in freshwater was from 2.41 in the second treatment to 2.69 in the first treatment and 2.56 in the third treatment. The average value CF in brackish water was 1.39. Survival rate in the freshwater treatment from the minimum to the maximum stocking density was 85.41, 82.21, and 82.76 % respectively and in brackish water it was 88.73.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Roach ; Rurilus rutilus caspicus ; Cultivation ; Brackishwater ; Freshwater ; Earthen ponds ; Fingerlings ; Specimens ; Density ; Feeding ; Survival rate ; Investigation ; Feasibility
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 25
    Publication Date: 2021-05-19
    Description: The Caspian Sea is an important source of water in terms of having valuable resources of sturgeon and bony fish is taken into consideration roach(Rutilus rutilus caspicus (L.)) is one of the most important commercial species in the southern coast of the Caspian Sea in Iran , the catch of this species has declined substantially in the last 10 years, The management and exploitation need of comprehensive review of its biology .This study as part of a comprehensive study of feeding , spawning and growth of this species.In this study, using samples caught in beach seine along the southern coast of the Caspian Sea coastal ( Iranian waters ) took place. The fork length and total weight ranged between 12.5 to29.5(cm) and 29 to293( grm),10.5 to23(cm) and17.2 to21(grm) in Golestan and Gilan Province respectively. The b value of the length-weight relationship ranged 3.02 to 3.25 and 3.28 to 3.75 for female and male, in Golestan and Gilan Province respectively. The age composition of the catch was from 1 to 4 year in both Province, there was one spawning peak and Fecundity variations were high and ranged 7260 to 231965 eggs. Average growth in length was described with the Von Bertalanffy growth model: L (t) = 30.94(1-exp (0.42(t-0.18) and L (t) = 20.49(1-exp (0.53(t-1). The percent of empty stomach and prey dominant evaluated during different seasons by specific formula. Result showed that gastropod, Polychaete worms and molluscs were dominated and specific food items respectively. Shrimp, fish, insects, zooplankton and clams were scare of prey.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rutilus rutilus caspicus ; Spawning ; Feeding ; Growth ; Length
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 26
    Publication Date: 2021-05-19
    Description: Bioaccumulation of heavy metals Cd, Pb, Cu and Zn in muscle tissue and organs (gills, liver, kidney and stomach) in A. persicus was studied in the ‘Iranian fishery zone 1’ lying between Astara (48? and 52' east longitude and 38? and 26' north latitude) and Kiyahshahr (49? and 53' east longitude and 37? and 27' north latitude). Samples were collected from five catch stations in the study area during the autumn catch season in 2001. A section of pectoral fin ray was removed for age determination. Samples were analyzed by wet digestion with hydrogen peroxide and nitric acid). Analyses were testified using spike method. A sample of cow liver (CMR 185R) was also tested to ascertain reliability of analyses. Digested samples were analyzed chemically using Flame Atomic Absorption Spectrophotometer (Varian spectra). Statistical analysis of results was carried out using ANOVA, Duncan’s test and test of clusters to determine differences between the mean concentrations of metals in terms of tissue, sex and region. The bioaccumulation pattern for Zn in different tissues studied was stomach〉liver〉kidney〉gills〉muscle tissue. Mean concentrations for Zn in the stomach was 136.6 ± 10.70 µg g -1 dry weight and belonged to a separate group as compared with other tissues (sig. Level 0.0000, F=26.6). However no significant differences were observed in the concentration of Zn in terms of other factors (sex, age, catch station). Cu with the maximum concentration of 39.71 ± 8.85 µg g -1 dry weight in liver showed a bioaccumulation pattern of liver〉kidney〉stomach〉gills〉muscle tissue. On the basis of Tukey test liver belongs to a separate group in terms of Cu concentrations. The bioaccumulation pattern for Pb was determined as gills〉liver〉kidney〉stomach〉muscle tissue. Maximum mean concentrations of Pb 6.87 ± 2.25 µg g -1 dry weight belonged to gills. According to Tukey test significant differences were observed among the various tissues studied regarding concentrations of Pb. Bioaccumulation pattern for Cd in the different organs studied in A. persicus was kidney〉liver〉gills〉stomach〉muscle tissue. Maximum mean concentration of 5.1 ± 0.97 µg g -1 dry weight belonged to kidneys and the minimum mean concentration of 0.05 ± 0.007 µg g -1 dry weight belonged to muscle tissue. On the basis of international standards the concentrations of Cd in muscle tissue are lower than the maximum allowable concentration (MAC) for this metal for human consumption. Concentrations for Cd are lower than MAC in some organs and are higher than MAC in other organs studied. The observed concentrations do not pose health problems as these organs are not used for human consumption. However they may cause problems in terms of histology and physiology and need to be studied further. The accumulation pattern for metals in sediment samples in the study regions was Zn〉Pb〉Cu〉Cd. The concentrations for metals determined in sediment samples collected at 10 and 15 m depths in the present study are lower than levels that are considered harmful to the environment.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Feeding ; Accumulation ; Tissues ; Heavy metals ; Bioaccumulation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 42pp.
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  • 27
    Publication Date: 2021-05-19
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 138pp.
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  • 28
    Publication Date: 2021-05-19
    Description: In order to determination of feeding requierments, in first step hole bady analysis and some biological characteriestis of Barbus sharpyie was investigated in khouzestan provience in 2001-2004. In this study 413 benni (Barbus sharpyie) in length size range (30 to 440 mm) were caught from different resources in region (mainly Shadegan and Horalazim wet lands) Fishes were classified in different length groups for carcasse analysis, The highest protein was observed in (30 to 95 mm) and (100-140 mm) length size groups, and also the rate of protein decrease with Length increase The lowest lipid (body fat) rate was observed in (100-149 mm) length size group and also with decreasing the protein in length groups, lipid was increased in Benni (Barbus sharpeyi) body. The maximum rate of body ash was measured in (30-95 mm) length size group, after this with increasing of the length, the rate of ash decreased gradually, so the minimum ash rate was observed in (30-440 mm) length zise group. Phas phorous and calcium variations were the same as ash changing the maximum energy was obtained in (250-350 mm). That was in harmony with increasing in this length groupe. Falty acids in Benni bady were higher then X-3 and were similar to falty acids profiles in carp fishes bady Benni protain in cludes Essential amino acids, Arginine, Histidine, Iucine, Methionine, Valine, Phenylalanine, theronine, Lysine, that were the same in different individual. Regarding length weight relation. Benni growth is Allometric and regarding stomach contents and increasing of gut length Index in different length size groups, its feeding habits is herbivorous .
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Barbus sharpeyi ; Body analysis ; Feeding ; Biological
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 29
    Publication Date: 2021-05-19
    Description: The Caspian Sea is the biggest lacustrine water source. Common carp is very important commercially in south coastal line of Iranian Caspian Sea waters, especially in east coastal line, because eighty percent annual catch of this species has been caught in this area in particular. Despite the common carp importance and value in Iran, but there is a little information about its biology in natural system. This study is comprehensive research on reproduction, growth and feeding. The specimens were caught by beach seine net in coastal line of Iranian waters. The fork length ranged between 5.6 and 2866.2 g. The b value of the length-weight relationship ranged from 2.843 to 2.924 for female and male, respectively. The age composition of the specimens was from 1 to 11 year. The gonadosomatic ratio (GSR) changed from 2 to 12. There were two peaks of GSR in April and December, so that the latter peak was much shorter than the first that. Fecundity variations were high and ranged 77448 to 430745 eggs. It observed linear significant correlation in fecundity- weight (r=0.98) and fecundity-length (r=0.88) relationships. Average growth in length was described with the Von Bertalanffy growth model: L (t) = 60.5(1-exp (0.19(t-0.65). The percent of empty stomach and prey dominant evaluated during different seasons by specific formula result showed that in Cyprinus carpio prey Molluscs was dominated and specific food items. Empty stomachs in winter and spring were higher and lower, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cyprinus carpio ; Spawning ; Feeding ; Growth ; Common carp ; Biology ; Age ; Coastal line
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 30
    Publication Date: 2021-05-19
    Description: In the changing environmental of the Caspian Sea specifically pelagic zone following the introduction of the comb jelly Mnemiopsis leidyi has been continued ,particularly kilka catch ,biological characteristic and feeding . In this investigation which had been done in commercial catch regions ( where discharged theirs catch) in three ports Babolsar ,Amirabad (in Mazandaran) and Anzali (in Guilan ), estimated catch and catch per unit effort , the age structure of catch ,length- weight relationship , von Bertalanffy growth parameters , condition factor ,sex ratios ,maturity stags ,age at first capture and spawning and feeding .The result shown that catch of kilka in Iranian coastal in 2006 decreased from 22300 ton to 16700 ton in 2008.But CPUE increased from1.7 ton (Vessel ×Night) to 2.5 ton at that time due to dropped effort .The length frequency of kilka is close and the juvenile fish did not see in catch as increased the length mean (P〉0.000).The age frequency had the same situation as fish low than 2 years did not see for anchovy and bigeye ,so collapse juvenile stock cause poor recruitment in the species .The parameters of the von Bertalanffy growth curve were For common kilka L∞= 136.5mm k= 0.249y t0= 1.890y Q= 3.883; For bigeye kilka L∞= 131.7mm k= 0.346 t0= 1.123y Q= 3.688; And for anchovy kilka L∞= 148mm k= 0.375 t0= 1.243y Q= 3.814 The exploitation rate of anchovy, bigeye and common kilka were 0.51, 0.58 and 0.6 respectively. The highest of GSI recorded in May and March for common and bigeye and in Jul, Nov for anchovy kilka .Main prey was Acartia tonsa by anchovy and common kilka and Cypris balanus by bigeye kilka. We concluded that the ecological problem causes by Mnemiopsis leidyi as well as overfishing has been collapsed kilka stocks, particularly anchvy.so sustainable fisheries management and conservation biodiversity in the Caspian Sea are real challenges now.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Environmental ; Commercial ; Ecological ; Kilka fishes ; Age ; Growth ; Feeding ; Overfishing ; Pelagic zone ; Mnemiopsis leidyi ; Bertalanffy ; Sex ratios ; Maturity ; Spawning ; CPUE ; Acartia tonsa ; Cypris balanus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 98pp.
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  • 31
    Publication Date: 2021-05-19
    Description: Intensive culture of rainbow trout, usually done using several different methods.The objective of this study was to compare fish performance and welfare in a new design of Foster-lucas ponds (FLP) and a raceway ponds (RP) under field conditions. Currennt study was designed in two treatment with three repeats. Each pond was randomly stocked by rainbow trout fish with initial average weight of approximately 51±5 g. Stocking density was 120n/m3 during study. After 10 months of trial, fish fed diets with comertial diet (Chineh Company). Feeding operation conducted according to the manuals by consideration of water temprature, fish waight and biomas. During the 10 months experiment, the fish survival rate was high (97.39 and 98.05%). No significant differences in growth parametres were observed between RP and FLP, but in all parametres FLP was higher than RP Final weight was 0.99% higher in FLP than in RP. Water quality factors (Dissolved oxygen, PH) was better in FLP. The results confirm that when water quality is maintained in safe level ranges, growth rate of rainbow trout attained in FLS are comparable to those in RP.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Rainbow trout ; Oncorhynchus mykiss ; Fish culture ; New design of Foster-lucas pond ; Raceway ponds ; Culture ; Stocking ; Density ; Diet ; Feeding ; Temperature ; Biomass ; Growth ; Dissolved oxygen ; pH
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 39pp.
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  • 32
    Publication Date: 2021-05-19
    Description: Aspects of the biological features such as age and growth , the reproductive cycle , food and feeding of the Longnose Trevally (Carangoides chrysophrys) were studied from a total 376 specimens collected by use of trawl fishing in Hormuzgan waters between February 2014 and February 2015. The minimum and maximum total length during different months were between 25.5 and 80 cm respectively. Weight-length relationship for Longnose Trevally was W = 0/0064L 2/9004. This fish had an isometric growth. Fishes aged using sections of their otoliths . The equation of growth for Longnose Trevally obtained Lt = 85(1-e -0/266(t+1/443) ). Total mortality rate for Longnose Trevally was 0.412. LM50 and TM50 for Longnose Trevally was 46 cm, 2 years. Sex ratio(femail : male) for Longnose Trevally was 1/42:1. Maximum absolute and relative fecundity for Longnose Trevally were 479992 and 354 respectively. The highest GSI in April (2.86) and the lowest was in June 2014 (0.43). It has a long spawning season from January to May and spawning peak was observed in May. Longnose Trevally was Relatively low feed (CV= 65.49). Main food for Longnose Trevally were bony fish(Fp= 91.67). Random diet of Longnose Trevally were crustaceans (Fp = 4.17) (shrimp, crab and squilla) and mollusks (Fp =4.17) (cutlle fish, Squid), respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Longnose Trevally ; Parameters ; Growth ; Spawning ; Season ; Length ; Mature ; Fecundity ; Feeding ; Sex raio ; Carangoides chrysophrys ; Biological
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 68pp.
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  • 33
    Publication Date: 2021-05-19
    Description: Investigation on small pelagic fishes in the north of the Persian Gulf (Qeshm Island and Bandar Lengeh) coastal waters during October 2006 to September 2008, illustrated that 6 sardine and 2 anchovy species were locally existed. Sind sardinella (Sardinella sindensis) and Buccaneer anchovy (Encrasicholina punctifer) were dominated in this area. The peak of sexual maturity for Sind sardinella and Buccaneer anchovy occurred in spring and summer respectively. Reproduction period for Sind sardinella was from the middle of winter to late spring, and Buccaneer anchovy encompassed reproduction capacity all the year. The female: male ratio was calculated 1:0.7 and 1:0.9 for Sind sardinella, 1:1.27 and 1:1 for Buccaneer anchovy in Qeshm Island and Bandar lengeh sequentially. The mean of absolute fecundity were estimated 16234 and 16168 for Sind sardinella, 1277 and 1141 for Buccaneer anchovy in Qeshm Island and Bandar Lengeh respectively. LM50 and LC50 were calculated and the results showed both species mainly caught before length of maturity, and the mass populations have not sufficient opportunity to reproduction. Both species were planktonivore, theirs food prefect mainly consisted of copepods, naplius and bivalves. Vacuity index indicated both spices were semi-voracious appetite fishes. Based on calculated length-weight relationships in S. sindensis and E. punctifer, they have isometric growth. Growth parameters were estimated, the results illustrated that they were growth fast and short live species, which suggested S. sardinella and E. punctifer have 3 and 2 years maximum life span respectively. The recruitment pattern indicated double recruitment peaks per year in both species. Annually, four cohorts were distinguished in Sind sardinella and 3 cohorts in Buccaneer anchovy. Total mortality (Z), natural mortality (M) and fishing mortality (F) were estimated in both species, and the values of exploitation ratio (0.78 & 0.74) in Sind sardinella and (0.72 & 0.41) in Buccaneer anchovy were reasonable for current fishing effort due their short life span. Analysis of the results showed that CPUE in sardine and anchovy affected by reproduction and feeding processes. Results showed significant correlation between sardine CPUE and Sardine GaSI (P〈0.05, r= 0.499), similarly between anchovy CPUE and anchovy GSI (P〈0.05, r=-0.635). Further, significant correlation between sea surface temperature with sardine CPUE (P〈0.05, r=0.493), phytoplankton distribution (P〈0.01, r=0.560), anchovy GSI (P〈0.05, r=0.526), anchovy GaSI (P〈0.05, r=-0.500) and chlorophyll a (P〈0.01, r=0.780). Phytoplankton distribution with sardine GSI (P〈0.05, r=0.453), zooplankton distribution with anchovy GSI (P〈0.05, r=0.671), and chlorophyll with sardine GSI (P〈0.01, r=0.761) have significant correlation.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sardinella sindensis ; Encrasicholina punctifer ; Reproduction ; Feeding ; Growth and mortality ; Catch per unit effort ; Sea surface temperature ; Chlorophyll a ; Sexual maturity ; Plankton ; Naplius ; CPUE ; GSI ; Sardine ; Anchovy ; Coastal waters ; Surface ; Temperature ; Distribution
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 134pp.
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  • 34
    Publication Date: 2021-05-19
    Description: The overfishing and changing environmental following the introduction of the comb jelly Mnemiopsis leidyi in the Caspian Sea, the population structure has a strong influence on kilka fishes in the Caspian Sea and therefore to in this investigation which had been done in commercial catch regions (where discharged theirs catch) in three ports Babolsar, Amirabad (in mazandaran) and Anzali (in Guilan), stimated catch and catch per unit effort, the age structur of catch, length- weight relationship, von Bertalanffy growth parameters, condition factor, sex ratios, maturity stags, spawning and feeding. The result shown that catch of kilka in Iranian coastal in 2012 decreased from 24080 ton to 22696 ton in 2013. But CPUE increased was 2.7 ton (Vessel ×Night) and there was no significant changes. Common kilka was the predominant all months of the year, 98.1 and 98.9 percent in 2012 and 2013 respectively. The mean length was 104.4±10.5 and 101.6±11.2 at the same time and range length 92/ 5 – 117/5 mm were the dominant population(about 75%). Relative abundance of fish less than 77.5 mm and more than 127.5 mm is very low. Age abundance during this period have not been substantially changed and always fishes with 3 and 4 year olds had the highest frequency73.6 and 73.5 percent respectively. The parameters of the Von Bertalanffy growth curve were ] exp1[8.141 ) 048.1(297.0  tLt for common kilka. The instantaneous coefficient of natural mortality (M) was 0.506 yr-1. The instantaneous coefficient of fihing mortality and total mortality were 0.694 yr-1 and 1.2 yr-1 , respectively. The exploitation rate of common kilka varied during 2012-2013 between 0.41-0.50. The mean condition factor 1.41±0.14 (n=5801) and 1.38±0.16 (n=6754) at the same time respectively . According to statistical analysis Anova oneway were a significant difference between condition factor at the same time . Main prey common kilka was Acartia tonsa accounting for over 80%. It seems Common kilka due to a decrease in the frequency of other zooplankton species depend more than ever on Acartia tonsa and this species is probably a concentration of prey for Common kilka.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Environmental ; Kilka fishes ; Age ; Growth parameters ; Condition factor ; Exploitation rate ; Feeding ; Reproduction ; Overfishing ; Comb jelly ; Mnemiopsis leidyi ; Population ; Sex ratios ; Maturity ; Spawning ; Mortality ; ANOVA ; Acartia tonsa
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 65pp.
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  • 35
    Publication Date: 2021-05-19
    Description: In this study, the inhibitory effect of Radio-Vaccine against white spot disease on white shrimp (Litopenaeus vannamei) was studied. This vaccine was prepared by Nuclear Science and Technology Research Institute of Atomic Energy Organization of Iran. 14,000 L. vannamei postlarvae were used in treatments after adaptation. Eight treatments, four positive and two negative controls were considered in triplicate. Initially, three meals a day for a month in the hours of 8, 16 and 24 were feeding. Three times in day at 8, 16 and 24 clock were feeding in first mount. Waste food and fecal remaining in the tanks were removed. In the second and third months of treatment, feeding frequency increased to three and four times in day which is at 6, 18 and 24 were given concentrate feed and Artemia nauplii at 12 clocks. Vaccination was carried out by immersion. Vaccination was performed in two doses with an interval of 10 to 14 days. For biometric testing and evaluation of immune cells throughout the experiment, three samples were taken randomly from the tanks. To assess the health status of shrimp, indices of Total Hemocyte Count and Total Plasma Protein was determined. Phagocytic activity of hemocytes was evaluated to assess immune setuation of shrimps. The results of this study showed that the shrimp immune system, as in many reports, the effect of recombinant vaccines and passive safety in memory as "Alternative adaptive immune responses" after experimental infection of white spot virus (WSSV) in wild and farmed shrimp is confirmed, will respond to vaccination against white spot disease.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Immunity index ; Litopenaeus vannamei ; White spot virus ; Disease ; White shrimp ; Feeding ; Artemia nauplii ; Samples ; Hemocyte ; Plasma Protein
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 36
    Publication Date: 2021-05-19
    Description: Two crop culture of western white shrimp with 2 treatments and 2 replicates in each treatments in earthen ponds with area 0.4 hectares at a density of 20 pl 18 in each square meter were done. For the culture of shrimp in the second stage, the post larvae cultured in nursery pond with area of 0.25 hectare, and density of 96 post larvae in each square meter, and then transfer to the rearing ponds. In each pond, 2 aerators with potency of 3 horsepower fixed. Every day horsepower clarified at 21p.m. to 23 p.m and out at 6 a.m to 7 a.m. The first stage of culture begun in 19 April with pl 18. The yield pick-up after 96 days. The stocking of pl in the nursery ponds was done in 25 July with pl16 and after 38 days hunted and transferred to second stage culture. The second culture was done in 1 September and pick-up after 65 days. For feeding of shrimps we used commercial food numbers 4001 until 4006. In the first mount the feeding of shrimps were done as blind feed and then with the shrimps biomass and with research of feeding trays. Water temperature, dissolved oxygen and pH daily in 2 times at the morning (6 a.m) and evening (at 15 p.m) and the salinity, transparency and water depth 1 time daily measured. In the first culture period (96 days), the mean weight, length, daily weight gain, FCR, survival percent shrimp production were 14.03 gr, 12.08 cm, 0.15 gr, 1.02 gr, 92 percent and 2132 kilograms and in the second culture period (65 days) 14.55 gr, 12.48 cm, 0.22 gr, 0.84, 73 percent and 2675 kilogarms measuered respectively. Totally, in the second period culture the initial weight of shrimps was higher than the first period culture 1.95 gr and 0.008 gr respectively, therefore growth indexes in the second period were better than the first culture period. Coefficient of determination of weight and lenth of shrimps with increase of cultue days, in the first and second culture periods, in the total cases the number were higher than 0.97. This shown that the role of increase of days on the increase of weight and lenth is higher than 97 percent. Also, determination of product moment, showed that there is very strong correlation between increase of days and increase of weight and lenth and between weight and lenth (0.97 to 0.99).
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Western white shrimp ; Two crop culture ; Earthen Pond ; Density ; Larvae ; Nursery pond ; Feeding ; Temperature ; Dissolved oxygen ; pH ; Salinity ; Transparency ; Weight gain ; FCR ; Survival
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 35pp.
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  • 37
    Publication Date: 2021-05-19
    Description: The present research aimed to study effect of Nereis worm in feeding, growth, survival and carcass biochimical compositions of persian sturgeon larvae. Five diets including zooplankton (100%) as the control (Z), Nereis diversicolor worm (100%), a mix of Nereis and zooplankton (50% for each), a mix of Nereis, zooplankton and concentrate food (33% for each) with 3 replicates were established. At first, Nereis worms were cultured up to the weight of 200 mg according to the protocol done in the International Sturgeon Research Institute. Required zooplanktons were obtained for Dr. Beheshti Sturgeon Rearing and propagation center. Concentrate food was also made in the ISRI. Persian sturgeon larvae with the average weight of 95.66 mg were put in 60-Liter tank (60 larvae in each tank) under the same physical and chemical parameters of water. 8 days after the beginning of active feeding, they were fed five times a day with experimental dites based on 20-30% of their weight for 15 days. Water quality parameters, such as dissolved oxygen, temperature, pH values were recorded daily. The average of temperature, pH and dissolved oxygen during the test were 22.8±1.3ºc, 7.5±0.1 and 6.58±0.9 mg/l respectively. At the end of this period, condition factor (k), specific growth rate (SGR), food conversion rate (FCR), weight gain (WG) and Body weight index (BWI) were calculated. Total fat and fatty acids profile were analyzed. The results showed that there was a significant difference between growth indexes and food consumption (p〈0.05). BWI, GR and SGR indices showed no significant difference between N and NZ treatment, but the average of these indices were higher in NZ treatment. The most and the least average of SGR, BWI were observed in NZ and NZC treatments respectively. Condition factor showed no significant difference in all diets except NZ. FCR had no significant difference between N and NZ as well as after diets. The highest and the lowest survival rate was observed in N treatment (96.11±1.46%) and NZC (85.55±3.37%) respectively. The results of carcass analysis showed that there is a strong correlation between fatty acids of body and fat resources of diets. According to the significant difference in growth rate between treatments (p〈0.05), the larvae fed with N diet presented a better performance compared with others. These results indicate that cultured sturgeon larvae need n-3 and n-6 series of fatty acids in their diet.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biochemical ; Physical ; Chemical ; Acipenser persicus ; Nereis diversicolor ; Growth ; Survival ; Fatty acids ; Larvae ; Feeding ; Persian sturgeon ; Zooplankton ; Dissolved oxygen ; Temperature ; pH ; SGR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 69pp.
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  • 38
    Publication Date: 2021-05-19
    Description: A study was undertaken to determine the effects of various dietary carbohydrate to lipid ratios on growth indices, feed performance and body composition of benni (Barbus sharpeyi) juveniles. Nine iso-nitrogenous (25% crude protein) and iso-caloric (3.5 kcal/g digestible energy) semipurified diets with varying carbohydrate to lipid (CHO L) ratios (0.8 to 8.8 g/g) were tested for 8 weeks with three replicates. Each replicate was stocked with 20 fish (initial mean weight 16.47 ± 0.25 g) that were fed to satiation thrice daily. Twenty seven flow-through fiberglass tanks of 300 L capacity with a flow rate of 1.5 L min-1 were used for rearing the fish. Maximum weight gain (WG), weight gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency ratio (FER), protein efficiency ratio (PER), apparent net protein utilization (ANPU) and fulton,s condition factor (K) were observed in fish fed a 44% carbohydrate and 9% lipid diet, corresponding to a CHO/L ratio of 4.8. Whole body moisture, crude protein and ash content of fish fed varying CHO:L diets did not show any significant differences among treatments (P〉0.05), But whole body lipid content of fish decreased (P〈0.05) as dietary CHO/L ratio increased. The hepatosomatic and viscerosomatic indices (HSI and VSI) decreased (P〈0.05) as dietary CHO:L ratio increased and maximum values were observed in diet with highest amount of lipid. The results of the present study indicated that the optimal dietary CHO/L ratio for a optimal growth and feeding performance of Barbus sharpeyi juvenile, ranged between 3.8 and 4.8.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Barbus sharpeyi ; Carbohydrate ; Lipid ; Growth ; Feeding ; Body composition ; B.sharpeyi ; Dietary ; Juvenile ; Weight gain ; Protein ; Hepatosomatic index ; Viscerosomatic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 49pp.
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  • 39
    Publication Date: 2021-05-19
    Description: This study was to investigate the possibility of pellet production for grow out common carp considering attractiveness and stability of the pellets. The experiment consisted of two periods and was conducted at the Caspian Sea ecology research center in 2011 and 2012. In period one, two types of pellet with similar protein level (31%) and different energy contents (3000 and 3500 cal/g) were formulated by the ingredients used in fish feed industry. In period two, 316 juvenile carp with initial weight of 27.9g were assigned randomly to 9 fiberglass tanks (4×4×1 m), with three replicates for each treatment. The fish were fed by three diets throughout the eight weeks study , two diets were formulated in the current study and third one was a commercial carp diet with 25% protein. The results showed that feeding with diet containing 31% protein and 3500 cal/g energy led to a feed conversion ratio of 3, but this value was recorded 1.9 for commercial diet. The type of diets was also influenced (P〈0.05) average final weight of the fish. Fish fed diet containing 31% protein and 3500cal/g energy showed largest weight gain and this value was significantly different from the other treatments. Pellet stability test showed that commercial pellet water stability was 3min, but this value for the two formulated pellet were 12 min. There was a highly significant difference for water stability between the commercial pellet and the formulated pellet. The result suggests that the formulated pellet in the current study can be easily replaced by commercial pellet.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Ecology ; Commercial ; Diet ; Pellet ; Carp ; Common carp ; Cyprinus carpio ; Rearing ; Protein ; Energy ; Juvenile ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 40pp.
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  • 40
    Publication Date: 2021-05-19
    Description: Catlassfishes are one of valuable stocks in the Oman Sea and Persian Gulf that need enough knowledge of biological and environmental characteristics of provinces. Thus this project was performed. In this study, the stock condition of Catlassfishes was considered from March 2000 to September 2001 in the Sirik and Hengarn area. Sampling carried out seasonally with bottom trawl. Dominant species was Trichiurus lepturus. Anal length compared in the both Sirik and Hengam areas. The results showed a significant difference (P〈0.05) between areas. Biomass and maximum CPUA were respectively estimated as 2856 tons and 3198 Kg/nm2 for Sirik area and 31 78 tons and 5204 kg/nm2 for Hengam area. IÄ and K were obtained i 15cm and 0.93, respectively. The instantaneous natural mortality co-efficient (M), Total mortality coefficient (Z) and exploitation ratio (E) was estimated as 3.65, It 15 and 0.65, respectively. Anal length per weight relationship obtained as 1 497X2*2849 2.2964 in Sirik and y=o.14649x in Hengam. This species seems to spawn throughout the year but two spawning peaks were determined in spring and Autumn. I-M50 was 27.5 cm. Female to male sex ratio was estimated 1.59 and showed a significant difference sexes. Minimum and maximum Fecundity were 2835 and 186000, respectively. Main food item was obtained sardine MSY values were estimated as 1642 and 1827 Ton for Sirik and Hengam respectively. Therefore, it is suggested that fishing should be done with care out of fishing season.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Trichiurus lepturus ; CPUA ; CPUE ; Biomass ; Growth parameters ; Reproduction ; Fecundity ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 85pp.
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  • 41
    Publication Date: 2021-05-19
    Description: Nowadays, the use of live food enrichment is very common in aquaculture and as a practical method introduced. Artemia a potential food source and quality is suitable, it will have used for feeding fish and crustaceans (shrimp). In this study, Artemia enriched with unsaturated fatty acids used for increase the sexual maturity of the white leg shrimp (Litopenaeus vannamei). Thus, the young brood stocks transferred to Bandarga Research station. In order to achieve optimal weight, Brood stocks were feeding by food concentrate and food frozen fresh. In this study such as 3 treatments each with 3 replicates, which brood stocks were stocking in the fiberglass thank with density 2 to 3 (pieces/m2). They were feeding with meals different including 1) squid , pen shale and marine polychaeta worm (Perinereis nuntia), 2) squid , pen shale and Artemia biomass "fransiscan", 3) squid , pen shale and Artemia biomass enriched with unsaturated fatty acid (HUFA) SPARI SELCO solution after were eye ablation. The results showed that sexual maturity increased in the brood stocks fed with Artemia enriched which with significantly higher brood stocks fed with Artemia biomass and the marine worm (P 〈0.05) whereas significant difference was not found in the rate of sexual maturity brood stocks fed with Artemia biomass and marine worm (P〉 0.05). The other hand, histological studies showed that brood stocks fed with Artemia enriched than other two treatments contain were many mature follicles ready for ovulation. However, there was more absolute fecundity in the brood stocks fed with Artemia enriched, but don't found any significant differences in competition with other two treatments (P 〉 0.05). In addition, during this period, measured and recorded all factors physicochemical water and environment daily. Following this study showed that if the Artemia enriched as part of the diet can both caused eliminate unsaturated fatty acid deficiency diet and improved ovarian development and increase sexual maturity brood stocks female in a short interval. Finally, the results analyzed with statistical software spss 9.0, one way ANOVA and Tukey test.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Histological ; Physicochemical ; White leg shrimp ; Litopenaeus vannamei ; Artemia ; Biomass ; Enrichment ; Sexual maturation ; HUFA ; Aquaculture ; Feeding ; Fatty acids ; Brood stocks ; Density ; Squid ; Polychaeta worm ; Perinereis nuntia ; ANOVA
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 45pp.
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  • 42
    Publication Date: 2021-05-19
    Description: The diet quality and type has a great role in aquatic animals and leads to increase of resistance against diseases and good growth . Cultured andornamental fish do not access to live and selected food due to captivity condition. Threfore, they should be provided with complete diet similar to natural food in captive condition. Carotenoid pigments are responsible of flesh pigmentation of edible fish and skin color of ornamental fish. The accumulation of this pigments in fish tissue has a greate importance in marketing and hence due to lack of its synthesis, carotenoids shoud be added to diet of cultured fish. As the synthetic carotenoids are harmful to the environment , there is a greate interest to use natural carotenoids in ornamental fish diets to obtain bright color. This study was carried out to compare the effects of diets containing Artemia urmiana and Phallocryptus spinosa supplements and commercial feed on growth and survival of goldfish fingerlings , quality of skin color, amounts of total carotenoids , Astaxantin, Canthaxantin and beta-carotene inCultured Carassius auratus during 90 days. The culture medium were contained glass aquaria in controlled condition and suitable for goldfish growth with 12 L: 12 D photoperiod and water temperature of 28 ± 1 oC. 3 test groups were included: treatment 1 fed with concentrate diet , treatment 2 fed with concentrate and frizzed Phallocryptus spinosa with tha same concentrations and treatment 3 fed with concentrate and frizzed Artemia urmiana with the same concentrations . Each treatment contains 2 replications and each replication consisted of 30 goldfish. . In this study, The amounts of total carotenoids using spectrophotometer modelWPA , astaxantin, canthaxantin and beta-carotene using HPLC model Younglin, UK, were determined in the skin of Carassius auratus at the end of the exprement period. The results revealed that the most growth rate ( GR), specific growth rate and condition factor ( CF) were 0.11 ±0.006 , 0.34 ± 0.015 and 3.96 ± 0.10, respectively which due to treatment 3 and the most weight gain and length gain including 8.57± 1.18g and 31.54± 3.33 mm, respectively due to treatment 2 .During rearing period , there was not any significant difference among treatments( p〉 0.05). The analysis of obtained data showed that there was a significant difference between diets containing live food and concentrate diet (p〈 0.05). The results revealed that live food enhanced skin color of Carassius auratus compared to concentrate diet . Also, the most pigmentation obtained from the diet contained fairy shrimp. As, in concentrate, concentrate and fairy shrimps, concentrate and Artemia diets amounts of total carotenoids at 450 nm wave length were 1.09, 3.90 and 2.07 mg/100 , asthaxantin were 84.57, 205.82 and 102.24 ng/g and canthaxanthin were 0.24, 35.79 and 30.64 ng/g and carotenoid were 34.73, 138.78 and 69.77 ng/g, respectively. The use of fairy shrimp compared to Artemia in the diet can be significantly increased the amounts of carotenoids especially asthaxanthin in the skin of goldfish(p〈 0.05). Therefore fairy shrimps can be used as a suitable for artemia and high cost synthetic pigments to enhance color of ornamental fish.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Carasius auratus ; Growth ; Survival ; Carotenoid pigments ; Artemia urmiana ; Phallocryptus spinosa ; Concentrate ; Fish ; Goldfish ; Survival rate ; Feeding ; Diet
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 43
    Publication Date: 2021-05-19
    Description: For the accomplish of project, nine treatments with three replicate in each treatment, used as follows. Control treatment: Feeding with natural foods (Chaetoceros sp and artemia nauplii) Treatment 1: Feeding with combination of inner artificial diet and natural foods. Treatment 2: Feeding with combination of imported artificial diet and natural foods. Treatment 3: feeding from zoea 1 to mysis 1 with chaetoceros sp and from mysis 1 to pl15 with inner artificial diet. Treatment 4: feeding from zoea 1 to mysis 1 with chaetoceros sp and from mysis 1 to pl15 with imported artificial diet. Treatment 5: feeding from zoea 1 to pl15 with project artificial diet. Treatment 6: feeding from zoea 1 to pl15 with imported artificial diet. Treatment 7: Feeding in zoeal stages with chaetoceros sp and from mysis 1 to pl15 with chaetoceros sp+artemia nauplii+ imported artificial diet. Treatment 8: Feeding in zoeal stages with chaetoceros sp and from mysis 1 to pl15 with chaetoceros sp+artemia nauplii+ project artificial diet. Larvae stocked at a density of 100 nauplii/ L-1 (1000 nauplii in each tank each tank), at the beginning of culture period The results showed that, in treatments 1 , 2, 7 and 8 from zoea 1 to pl15, growth indexes were suitable and noticible, and in most treatment better than control treatment. Survival percent, in pl15 in treatment 1, 2, 7 and 8 was more than control treatment, and differention between treatment 1, 2 and 8 in comparision with control treatment was statictically significant (p〈0.05). Survival percent in treatments 5 and 6 from stages zoea 1 to late zoea 3 stage, in comparision with other treatments were lesser, but without statictically significant (p〉0.05). But thereafter, significantly decresed. In treatments 3 and 4 from zoea 1 to late mysis 3 stage, survival percent in comparision with other treatments were suitable, and thereafter, significantly decreased, and with statictically significant with other treatments (p〈0.05). The means of final weight in treatments 1, 3 and 8 was more than control treatment, but without statictically significant (p〉0.05), but in treatment 8, was more than other treatments. The mean of final length between treatments 1, 2, 6, 7, 8 and in comparision with control treatment, were not statistically significant (p〉0.05). But in treatment 2, more than control treatment, and in other treatments less than control treatment.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Larvae culture ; Litopenaeus vannamei ; Imported artificial diet ; Inner microbound artificialdiet ; Feeding ; White shrimp ; Larval stage ; Chaetoceros Sp. ; Artemia nauplii ; Zoea ; Mysis ; Diet
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 40pp.
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  • 44
    Publication Date: 2021-05-19
    Description: To assess some biological aspects of croaker fish (Sciaenidae) in Mahshahr Creeks (Mousa Bay, persian Gulf) monthly sampling (Eight months) in four creeks namely Ghazaleh, ahmadi, Bihad and Moavi was conducted.....
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological Aspect ; Fish ; Spawning ; Feeding ; Diet
    Repository Name: AquaDocs
    Type: Report , Not Known
    Format: 121pp.
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  • 45
    Publication Date: 2021-05-19
    Description: Present study analyzed the stomach contents of individuals belonging to five fish’s species in order toInvestigate seasonal changes in feeding strategy and diet composition in the South coast of the Caspian Sea (Gilan and Mazandaran provinces) from late March until late May 2010 using the research ship Gillan and fishing with batoom trawl nets and fishing blades insoles bony fishes in the depths of less than 60 meters. The average length and the total weight of Rutilus frisii kutum, Rutilus rutilus caspicus, Cyprinus carpio, Liza auratus and Liza saliens respectively were 36/9 ± 8/5 cm , 562/9 ± 353/2 g, 23/2 ± 3/2 cm , 202 ± 101/6 g, 24/6 ± 8/1 cm , 249/8 ± 242 g, 32/2 ± 8/9 cm , 320/9 ± 242/8 g, 22/7 ± 2/6 cm and 82/9 ± 52/2 g repectively. Using the method of Shorygin and Costello highly nourishing food strategy and different species of fishes were studied. The results of this study showed that Rutilus frisii kutum fed generally of Balanus and Cerastorderma In order of importance after Bivalvia, Gastropoda, Crabs, Fish eggs, Filamentous alge in GI Rutilus frisii kutum presence, but feeding it of Bivalvia (in particular Cerastorderma) was much more than the other food. The results of present study showed that under the influence of compatibility and behavioral mechanisms, ecological, physiological and nutritional importance of Rutilus frisii kutum Bivalvia. The results of studies were showed that Bivalvia was feeding the dominant Rutilus frisii kutum , while the results of this study showed that despite Bivalvia present importance in feeding the of this species, it seems that its presence in such digestion decreased. As well as the dominant food of Rutilus rutilus caspicus was fish, but generally from Detrite fed. This study showed that feeding the dominant species of Cyprinus carpio, Liza auratus and Liza saliens of Detrite was. The most important food items after ditrit for the Cyprinus carpio in order of importance include the Bivalvia, Gastropoda, Oligochaeta, Abra ovata, Nereis, Fish eggs, Filamentous alge, was. The Liza auratus dietary habits also showed that after ditrit the most importance in nutrition related to Foraminifera, Porifera and Balanus, and for Liza saliens to Gastropoda, Bivalvia and Porifera. The results showed that the mullet the greater the intensity of feeding fishes than other species were, Rutilus frisii kutum in between of Cyprinidae the greater the intensity of feeding fishes than other species were (0/05 〉 P). The values of this indicator in the lower longitudinal in all fish species investigated were more than high longitudinal groups. The feeding intensity at different times of sampling for all species investigated showed that in all species at times extremely reduced the reproductive index (0/05 〉 P). As well as the diversity foods in the lower longitudinal in all fish species investigated were more than high longitudinal groups. In conclution, our results demonstrated Food strategy have changed based on physiological changes, metabolismi activity, behaviour, and the amount of food available.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Dietary habits ; Rutilus frisii kutum ; Cyprinus carpio ; Rutilus rutilus caspicus ; Mullets ; Survey ; Feeding ; Benthic ; Liza auratus ; Liza saliens
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 46pp.
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  • 46
    Publication Date: 2021-05-19
    Description: In this study, data were gathered from library research, discussing with feed and feeding experts in warm and cold water fish farms and also from research project implemented by executer. Standardization on selection of materials and ingredients percentage in order to prepare dry pellet feed for these two kind of cultured fishes emphasized on proximate composition energy, carbohydrate, protein with amino acids profile, lipid with fatty acid profile, minerals and vitamins and also feeding frequency, kind of feed pellet manufacture were codified environmental friendly, according to responsible aquaculture. On the other hand, feed color, taste, smell, their attraction and the best physico- chemical condition for storing of in order to get optimum result of responsible aquaculture were recorded.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Responsible Aquaculture ; Nutritional Standardization ; Coldwater fish ; Warm water fish ; Aquaculture ; Feeding ; Preparation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 103pp.
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  • 47
    Publication Date: 2021-05-19
    Description: This study was to assess the silver carp diet compositionduring grow out period. The experiment was conducted in a well-managed fish farm located at Mazndarn province from 2012 to 2013. Totally five samples from the pond water and also silver cap intestines were collected in May, April, Jun, July, August and September of 2012. The results showed that there are five phyla of phytoplankton in the ponds including: Phyrophyta, Bacilluryophyta, Cyanophyta, Clorophyta, Oglenophyta. A comparison of phytoplankton populationindicated a significant differences throughout the five sampling sessions (P〈0.05). Phytoplankton population increased gradually from April to September. The biomass of the phytoplankton was not also similar during the experiment (P〈0.05) and this value elevated along with increasing the phytoplankton number from April to September. The overall intestine content demonstrated that around 39.2% of the proximal intestine content composed ofChlorophyta . However Chlorophyta concentration reached to 34.9% for distal part of the intestine. The rest of silver carp intestinal phytoplankton were Bacilluriaphyta ,Cianophyta, Oglana and Phyrophyta, respectively.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Silver Carp ; Feeding ; Chlorophyta ; Bacilluriaphyta ; Cianophyta ; Oglana ; Phyrophyta ; Survey ; Hypophathalmichthys molitrix ; Samples ; Cyanophyta ; Oglenophyta ; Phytoplankton
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 44pp.
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  • 48
    Publication Date: 2021-05-19
    Description: A study was conducted to determine , feeding , reproduction in 12 stations in the Caspian Sea basin of Iran including Anzali wetland (lagoon), Ghorigol wetland, Neor lake , south Caspian coastal water (Iranian of shore), and several springs in Alborz mountains In addition sampling of Plankton and physical and chemical factors of the water body were also done in all stations. Special aquariums were designed to test the acclimation of the 7 amphipoda in pond culture. This process was performed from march 2001 to Feb 2003. The finding showed that while reproduction of Gammarus lacustris and Gammarus paricrenatus were only about 3 months , that Gammarus komareki lasted throught the year. From diet studies of intestine content have been showed that there is not a correlation between abundance and diversity of plankton with habitat for all species, except about Pontogarnrnarus maeoticus and P. borceae from Kelachi coast that had a high plankton abundance. Gammarus aequicauda (37.75 ± 31.04) and Obesogammarus acuminatus (49.07 ± 40.4) had the maximum of average eggs production while G. komareki had the lowest clutch sizes with mean about 7-8 eggs. Species tolerance to changes in environmental factors (Physical and chemical variation) were limited in the springs while those off coastal water species and Obesogammarus acuminatus were wider. In aquarium (laboratory) study, it was found that although Gammarus lacustris showed some growth but did not reproduce. Gammarus paricrenatus, in later stage of study, reproduced and grow in length during most experiment period. Although Gammarus komareki survived and a little active life, but was not able to acclimate itself to laboratory condition. The findings showed that four species P. maeoticus, P. borceae, O. acuminatus, and G. aequcauda , seems to be good candidate for acclimation study in warm water fish farms. Their living in the ponds can be enhanced by some limited alteration in substrate structure (P. maeoticus, P. borceae), presence of submerged aquatic plants (O. acuminatus) and higher water salinity (more than 2 ppt) for G. aequcauda..
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Gammarus paricrenatus ; Gammarus lacustris ; Gammarus komareki ; Obesogammarus acuminatus ; Feeding ; Biological ; Gammaridae ; Amphipoda ; Fish Culture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 157pp.
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  • 49
    Publication Date: 2021-05-19
    Description: Shrimp diseases, especially viral diseases are the major limiting factors of shrimp farming activities in the world. White spot disease is one of the most pernicious viral disease that lead to serious loss in all shrimps of penaeids family. In Iran during 2002 to 2004 in Khuzestan province and in 2005 in Bushehr province, the most ponds and farms infected with white spot and the entire industry faced threat of closure. Surface proteins of virus has important role in the early stages of virus with host cell interaction, because these proteins usually enumerated as a candidate to produce a subunit and recombinant vaccines. On the other hand, these proteins can use as an important virus antigen and produce virus-specific antibodies in the preparation of diagnostic kits. In several studies proteins in the form of injection or oral vaccine has shown promising results. This study was designed in order to gain these proteins for immunization of white leg shrimp. To achieve the white spot virus genome, sampling and DNA extraction of moribund shrimps with clinical signs was done from Choebdeh farms. VP28 and VP19 protein-coding gene-specific primers were selected, and after amplification and purification were cloned in E.coli TG1. Protein expression evaluated and commercial plates were coated with inactivated bacteria containing the recombinant protein. White leg shrimp post larvae (PL30) were fed with recombinant food for 7 days then two times in 9th and 23th day after beginning of plate feeding and was challenged by white spot disease virus. The results of the first exposure (end of the seventh day after the shrimp fed with recombinant plate) showed that the lowest mortality at day 14 after exposure in different groups belong to VP28 group with cumulative mortality percent %30 ± 3.84 and the highest percentage of the Group TG1 equal to %72.22±2.93, also The highest and lowest relative percent survival, belong to the group of VP28 (60.29± 5.09%) and TG1 (4.41 ± 2.94%), respectively. In terms of mortality percent and relative percent survival, VP28 and VP 28+19 has significant difference with other groups while there was no significant difference between the groups VP19, TG1 and pMal. In the second exposure experiment (21 days after end of plate feeding) results showed that the lowest cumulative mortality in different groups belong to VP28 (%50 ± 5.09) and the highest percentage was owned by Group TG1 equal to %75.55±2.22, also the highest and the lowest of relative percent survival belong to VP28 (%32.86± 6.83) and TG1 (%0.00 ± 2.98) groups respectively. Similar to the first experiment, VP28 showed significant differences in cumulative mortality and relative percentage of survival in contrast to other group. As a general conclusion it can be noted that recombinant protein VP28 in any form that delivered to shrimp was able to protect shrimp against white spot virus. On the contrary VP19 has no this ability. The survival rate is directly related to the duration of the presence of recombinant protein in the diet.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Commercial ; Shrimp ; WSSV ; Vaccine ; In vitro ; White spot disease ; Proteins ; DNA ; Larvae ; Mortality ; Survival ; Feeding
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 50
    Publication Date: 2021-12-05
    Description: Regarding the fish farming in cages, taking into account all nutritional requirements appropriate to the needs of aquaculture is essential in order to minimize damage to the environment and achieve maximum growth. Fish nutritionists should be aware about the analysis of ability to digest and absorb nutrients in the diet to prepare and adjust the diet according to the needs of aquaculture. Food must also have appropriate physical properties for food intake with minimal loss of nutrients in the water. Inadequate resources with low quality or energy consumption within food, reduced the growth performance and increased the excretion of nitrogen in the environment. Food must be designed so that the balance of minerals in food will help maintain osmotic pressure within the body of fish in salty water. Food requirements and efficiency of food conversion ratio (FCR) is variable by changing environmental conditions (dissolved oxygen, temperature, water quality, flow rate, light intensity ,day length). The fish of cages are feeding by floating pellets which have the ability to stand in the water column. The floating pellets allows the fish to have sufficient time and opportunity for finding food. But sinking pellets pass rapidly from the cage and are inaccessible to fish. According to the Caspian sea conditions is better the extruded food equipped with Hygenizer to be used that a portion of it remains on the water surface and part of it moves gently down with an immersion mode in the water column (Softly deposited) for fish consumption. The amount, number and time of feeding are important factors to assess the nutritional activities. In general, fish feed from dry food between 2 to 3 percent of their body weight. Food consumption was also varied due to the quality of the food and fish physiological factors such as age, size, stage of life and stress level. Fish food distribution being fed manually or by means of demand, launcher and automatic feeding. The farm managers should pay attention to the things like water flows in the cage, wind, fish appetite, consolidated flows in the cage during food distribution as well as food storage in dry places with proper ventilation system to prevent the growth of fungi and insects activities to prevent loss of food. Also, adding antioxidants to the 100150 ppm when storing dry foods including the important cases particularly in prevention of fish liver lipoidosis disease is considered.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Cage culture ; Salmon ; Feeding ; Analyze ; Nutrition ; Variety ; Fish ; FCR ; Aquaculture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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