Publication Date:
1979-03-23
Description:
When incubated in a calcium-free medium, isolated rat fasciculata cells showed neither an increase in the concentration of guanocine 3',5'-monophosphate (cyclic GMP) nor an increase in corticosterone production in response to adrenocorticotropic hormone (ACTH). In response to submaximum and maximum steroidogenic concentrations of ACTH, corticosterone formation was directly proportional to increases in calcium concentration ranging from 0 to 2.5 mM. Higher concentration of calcium, however, inhibited maximal ACTH-induced steroidogenesis. In the absence of ACTH, calcium did not stimulate cyclic GMP accumulation and corticosterone formation. ACTH-induced corticosterone synthesis, preceded by an increase in cyclic GMP, was restored when ACTH and calcium were both present in the medium. Cyclic GMP or dibutryl cyclic GMP-induced steroidogenesis was substantially reduced in the absence of calcium, but in contrast to the ACTH effect a significant amount of corticosterone formation occurred without calcium. It is proposed that at the physiological concentrations of the hormone, calcium regulates the transduction of information between hormone receptors and guanylate cyclase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Perchellet, J P -- Sharma, R K -- New York, N.Y. -- Science. 1979 Mar 23;203(4386):1259-61.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/34216" target="_blank"〉PubMed〈/a〉
Keywords:
Adrenal Cortex/*drug effects/metabolism
;
Adrenal Cortex Hormones/*biosynthesis
;
Adrenocorticotropic Hormone/*pharmacology
;
Animals
;
Calcium/*pharmacology
;
Cyclic GMP/*pharmacology
;
Dibutyryl Cyclic GMP/pharmacology
;
Dose-Response Relationship, Drug
;
Drug Interactions
;
Guanylate Cyclase/metabolism
;
In Vitro Techniques
;
Models, Biological
;
Rats
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
