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  • Triticum aestivum  (105)
  • Calcium  (98)
  • Springer  (202)
  • Alfred Wegener Institute for Polar and Marine Research & German Society of Polar Research
  • Hamburg: Verlag Weltarchiv
  • 1980-1984  (202)
  • 1935-1939
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Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 36 (1984), S. 139-144 
    ISSN: 1432-0827
    Keywords: 1,25(OH)2-vitamin D ; Weight loss ; Phosphate ; Calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary During a review of 42 metabolic studies in healthy women and men we observed that serum 1,25-(OH)2-D concentrations were directly correlated to the observed daily changes in body weight (r=0.68;P〈0.001) and to caloric intake/kg/day (r=0.39;P=0.01). These relationships could not be accounted for by related and physiologically expected changes in serum Ca or iPTH concentrations. However, serum 1,25-(OH)2-D concentrations were observed to be inversely correlated to serum PO4 levels (r=−0.44;P=0.004). In addition, serum PO4 levels were inversely correlated to the daily changes in body weight (r=−0.40;P=0.009). Since dietary sodium intake averaged 142 mmol/day, it is unlikely that the observed changes in weight were the result of changes in salt and water balance. Thus it seems reasonable to speculate that serum 1,25-(OH)2-D concentrations may vary directly with energy balance, as reflected by changes in body weight. This effect may be mediated by alterations in PO4 metabolism. The accurate assessment of serum 1,25-(OH)2-D levels thus appears to require several measurements over time periods during which body weight is stable.
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  • 2
    ISSN: 1432-0827
    Keywords: Electromagnetic field ; Bicarbonate ; Phosphate ; Calcium ; Fluoride ; Osteogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The present studies are aimed at establishing molecular correlations in the interaction of very low frequency electromagnetic fields with biological systems. Ca-uptake by chick embryo tibia rudiment in short-term culture was a useful model. Tibiae of 8- to 10-day-old chick embryos were incubated 60 min in simplified culture media in the presence of45Ca at 37.5±0.5°C either inside or outside pulsating electromagnetic fields. Radioactivity count in the medium was the most accurate method for determining Ca-uptake by the rudiment. The effect of the fields on the Ca-uptake depended markedly on the chemical composition of the culture medium: bicarbonate was indispensable; glucose or sucrose was important; phosphate was potentiating; ethanol, Mg2+, and NaF were stimulating. The field had no effect in (a) blank medium without tibia, (b) tibiae that had been altered by fixation with aqueous glutaraldehyde, (c) nonliving artificial systems endowed with great or small ion sorption capacity. The unique bicarbonate effect with living systems and the passive behavior of nonliving ion sorbing systems prompt the suggestion that the electromagnetic field probably couples with specific processes, such as a bicarbonate-dependent Ca2+ ATPase and the active ion transport, at the cell membrane level. The molecular mechanisms remain to be established.
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  • 3
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    Calcified tissue international 36 (1984), S. 194-199 
    ISSN: 1432-0827
    Keywords: Benzo(B)Thiophene-2-Carboxylic Acid ; Bone cells ; Calcium ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The purpose of the present study was to investigate the mechanism of action on bone of Benzo(B)Thiophene-2-Carboxylic Acid (BL-5583). BL-5583, at a dose range of 0.01–100 µg/ml, inhibited spontaneous as well as A23187 and PTH-induced bone resorption in tissue culture. This compound also decreased calcium uptake in both osteoclastic and osteoblastic enriched bone cell populations obtained by sequential collagenase digestion of 1–2 day newborn rat calvariae. The decrease occurred after a 5 min. incubation with45Ca and BL-5583. The effective dose range was 0.01–100 µg/ml. No effect on leucine incorporation or lactic acid production by bone cells was observed. BL-5583 also induced a transient decrease in calcium uptake in skin cells isolated from fetal rats by collagenase digestion, suggesting a lack of tissue specificity for this compound. No effect on cyclic AMP in isolated bone cells was observed with the same dose range that produced a calcium effect.
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  • 4
    ISSN: 1432-0827
    Keywords: Calcium ; Vitamin D deficiency ; 1,25(OH)2D3 ; Parathyroidectomy ; Parathyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Studies presented here were designed to investigate further the basis for an impaired cAMP response to parathyroid hormone (PTH) in osteoblastlike calvarial bone cells isolated from vitamin D-deficient rat pups. The goal was to perturb Ca, PTH, and vitamin Din vivo in order to see which factors might be responsible for the impairedin vitro bone cell cAMP response. Pups either were parathyroidectomized (PTX) 3–5 days, implanted with osmotic minipumps delivering high doses of PTH, given repeated, high doses of 1,25(OH)2D3, or were D-deficient (-D, i.e., born and suckled by D-deficient mothers). Osteoblastlike bone cells, isolated by sequential enzyme digestion and centrifugation, were exposed to PTH for 5 min in the presence of a phosphodiesterase inhibitor. In bone cells isolated from -D rat pups, both basal and PTH-induced cAMP accumulation were significantly lower than in +D bone cells. Earlier, we had shown that two daily injections of -D pups with 50 ng 1,25(OH)2D3 restores this reduced bone cAMP response of -D pups toward normal. In the present study, neither basal nor PTH-induced bone cell cAMP accumulation was affected by subjecting D-replete pups to PTX, PTH infusion, or repeated high doses of 1,25(OH)2D3 despite the fact that each treatment markedly changed serum Ca or serum immunoreactive PTH. The results indicate that the impaired bone cell cAMP response seen in -D pups is not a direct result of chronic hypocalcemia and that the “heterologous desensitization” seenin vitro with added 1,25(OH)2D3 could not be duplicated byin vivo treatment of +D pups with supraphysiologic doses of 1,25(OH)2D3. Finally the lack of alteration in the bone cell cAMP response to PTHin vitro after chronic PTH infusionin vivo fails to support the notion that the impaired response in -D bone cells can be explained entirely by “homologous desensitization” induced by high circulating levels of PTH in the hypocalcemic, -D rat pup.
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  • 5
    ISSN: 1432-0827
    Keywords: Calcitonin ; Parathyroid hormone ; Calcium ; Newborn ; Fracture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Determinations of serum calcium (Ca), phosphorus (P), calcitonin (CT), and parathyroid hormone (PTH) were carried out in 36 full-term newborn infants with fracture of the clavicle (CF) and in 46 normal neonates (N). At the 6th hour of life the CF neonates demonstrated lower serum Ca and higher serum CT in comparison with normal infants. In the hours following, no significant differences between the two groups for the Ca levels were found, whereas serum CT remained significantly higher in the CF newborns at the 24th, 48th, and 72nd hour of life. Significant differences between normal and CF infants in the PTH serum levels were detected only at the 48th hour, when PTH was lower in the CF newborns. The results of this investigation indicate that the fracture of the clavicle is a significant and peculiar factor in stimulating CT secretion. Serum Ca level appeared to be controlled by CT rather than auto-regulating the secretion of the hormone.
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  • 6
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    Calcified tissue international 36 (1984), S. 596-603 
    ISSN: 1432-0827
    Keywords: Calcium ; Ameloblasts ; X-ray microanalysis ; Transport ; Frozen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Energy dispersive X-ray microanalysis was applied to freeze-dried blocks of enamel organ tissue to determine levels of calcium in various celular regions. The tissue blocks were dissected free from adjacent forming enamel following injection of cobalt or fluoride ions, both of which temporarily inhibit enamel mineralization. In all control and experimental specimens there was an increasing gradient of calcium from the stratum intermedium cells to the distal ends of the ameloblasts. Calcium levels were significantly reduced near the distal ends of the ameloblasts following cobalt or fluoride injection as compared with controls. It is suggested that evidence of an intercellular buildup of calcium near the distal ends of the ameloblast supports a controlling function of these cells. The changes in calcium levels are correlated with alterations in mineralization known to occur in the adjacent enamel of the model systems employed.
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  • 7
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    Calcified tissue international 36 (1984), S. 77-82 
    ISSN: 1432-0827
    Keywords: Osteogenesis ; Diffusion chambers ; Alkaline phosphatase ; Calcium ; Phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The alkaline phosphatase activity and the calcium and phosphorus content of osteogenic tissue formedin vivo following the implantation of diffusion chambers loaded with rabbit bone marrow cells is reported. (In this study the term osteogenic includes osteoblastic and chondroblastic.) Chambers examined 14–70 days after implantation revealed progressive accumulation of mineral. Alkaline phosphatase activity increased until day 30 and declined thereafter. The osteogenic potential of the marrow cells decreased with increasing weight (age) of the cell donor rabbit when measured either as the percentage of chambers containing osteogenic tissue or as the amount of calcium, phosphorus, or alkaline phosphatase activity within the chambers. The results confirm that measurements of these parameters in tissue formed by cells incubated in diffusion chambersin vivo may be used as a method for assay of osteogenesis.
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  • 8
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    Calcified tissue international 36 (1984), S. 269-273 
    ISSN: 1432-0827
    Keywords: Bone loss (osteopenia) ; Calcium ; Corticosteroids (glucocorticosteroids) ; Fluoride ; Vitamin D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary To evaluate the effect of prednisone and triple treatment (sodium fluoride, calcium, and vitamin D) on trabecular and cortical bone serial bone mineral content (BMC) measurements were made at a metaphyseal (BMCD) and diaphyseal (BMCP) site on the forearm on 31 consecutive and previously bone-healthy patients scheduled for at least 24 weeks high-dose prednisone treatment. The patients were randomized into two further treatment groups: group I (n=16) received prednisone plus triple treatment and group II (n=15) received only prednisone. The two groups were similar with regard to age, sex, prednisone dose, and initial BMC. During 24 weeks treatment, BMCD (partially representing trabecular bone) and BMCP (mainly representing cortical bone) fell significantly and similarly, demonstrating that there is no preventive effect on bone mineral loss on the triple regimen. The BMC fall after 12 weeks was significantly more pronounced for metaphyseal (partially trabecular) than for diaphyseal (cortical) bone, whereas the values did not differ significantly after 24 weeks; this indicates a greater sensitivity to the hormone treatment of trabecular bone. In the entire group, the fall in BMC correlated positively with individual prednisone dose, significant at the diaphyseal site (r=0.39,P〈0.05), but not at the metaphyseal site (r=0.31, P=0.08). It is concluded that corticosteroid-induced osteopenia is a diffuse bone disease which affects trabecular as well as cortical bone, suggesting that BMC measured on the forearm reflects changes in bone mineral at other locations.
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  • 9
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    Calcified tissue international 36 (1984), S. 392-400 
    ISSN: 1432-0827
    Keywords: Vitamin D ; Chick embryo ; Bone ; Calcium ; Phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Chick embryos were injected in the yolk sac at various ages with various doses of different vitamin D3 metabolites. Serum concentrations of total calcium and inorganic phosphate were determined 24 h after the injection and histological and electron microscopic studies of the tibiae were conducted 3–6 days after. Confirming previous results, the injection of 1,25(OH)2D3 was found to produce significant hypercalcemia and hypophosphatemia. The dose required to produce these effects decreased with age: 100 ng on the 9th day, 50 ng on the 11th, and 10 ng on the 15th. This finding is interpreted as resulting from the fact that the specialized cells in the chorionic epithelium which are considered to be involved in mineral resorption from the shell differentiate between the 11th and 13th days. Although no bone changes were observed in embryos injected before the 11th day, a rim of unmineralized trabeculae (osteoid) was observed at the periphery of the cortex of the tibial diaphysis in the embryos which had been injected after that age. Thus, in embryos injected on the 11th day with 100 ng 1,25(OH)2D3, the trabeculae formed between the 11th and 14th day remained unmineralized until the 15th or 16th day at which time they completed their mineralization. In the embryos injected on the 14th day, the alterations were more severe and could be produced with doses 10 times smaller than those required when the injections were made on the 11th day. At all ages, the doses that produced an osteoid rim also induced hypercalcemia and hypophosphatemia. The electron microscopical study of the osteoid trabeculae showed that osteoblasts and osteocytes had normal cytological characteristics and that the bone matrix did not present changes other than the reduction in mineral deposition. While the above findings do not exclude a direct action of 1,25(OH)2D3 on bone cells as the mechanism of osteoid formation, they do underline the importance of the humoral changes at least as partial determinants of this phenomenon. The activities of various vitamin D metabolites were compared using as parameter the threshold-dose required to produce a rim of unmineralized trabeculae in the tibia of 14–15 days embryos (T-D). The most active metabolite appeared to be 1,25(OH)2D3 (T-D: 10 ng); it was followed by 1,24,25(OH)3D3 (T-D: 100 ng) and 1,25,26(OH)3D3 (T-D: 100 ng). Vitamin D3 itself (T-D: 100 µg), 25(OH)D3 (T-D: 2.5µg) and 24,25(OH)2D3 (T-D: 5 µg) produced similar responses but only when administered in much larger doses.
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  • 10
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    Calcified tissue international 36 (1984), S. 702-710 
    ISSN: 1432-0827
    Keywords: Corticosteroids ; Cartilage ; Organ culture ; Calcium ; Matrix vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The present study examined the effects of various corticosteroid and noncorticosteroid hormones upon the ultrastructure of chondroprogenitor cells and chondroblasts in an organ-culture system of late fetal condylar cartilage. Corticosteroids, (triamcinolone acetonide, dexamethasone, corticosterone) at concentrations of 10−6–10−8M stimulated markedly a precocious formation of matrix vesicles by chondroblasts. This stimulation was accompanied by a significant accretion of calcium complexes intra- and extracellularly in both the chondroprogenitor cell population and chondroblastsin vitro, as well as in the newly induced matrix vesicles. Nonglucocorticoid steroids (progesterone, estradiol, testosterone, cortexolone) did not evoke similar effects. Progesterone and testosterone, however, seemed to adversely affect the ultrastructure of the cartilage cells, whereas estradiol appeared to have a favorable effect on the morphology of cultured condylar cartilage.
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  • 11
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    Archives of microbiology 138 (1984), S. 140-152 
    ISSN: 1432-072X
    Keywords: Caulobacter ; Prostheca development ; Stalk function ; Calcium ; Phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Calcium was found to stimulate stalk development in Caulobacter crescentus and to relieve the inhibition of development long known to be caused by phosphate. This suggested that phosphate inhibition could be attributed to its interaction with Ca2+, thereby depriving the cells of a factor that promoted development. Calcium was also found to promote phosphate acquisition by the cells, observed as acceleration of growth at extremes of phosphate concentration, as promotion of carbon-source utilization rather than storage, and as support for phosphate-dependent resistance to arsenate inhibition of growth. Cytological studies of dividing cells revealed that stalked siblings had greater access to exogenous phosphate for use in growth or for storage as polyphosphate, and that access of non-stalked sibling to phosphate was dependent on the length of the stalk of the dividing cell. It was concluded that the physiologic role of the stalk is enhancement of phosphate acquisition. The stimulatory role of calcium in this process was attributed to its support of stalk development and to its stabilization of internal membrane/cell envelope association within the cell-stalk juncture.
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  • 12
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    Archives of microbiology 139 (1984), S. 102-104 
    ISSN: 1432-072X
    Keywords: Fungus ; Zoospore ; Blastocladiella ; Allomyces ; Gamma ; Particle ; Phosphate ; Calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elemental composition of gamma particles in zoospores ofAllomyces macrogynus andBlastocladiella emersonii was determined by use of energy dispersive X-ray analysis of glutaraldehyde fixed, thin section zoospores. Isolated preparations of gamma particles were also examined. Gamma particles contained no detectable elements. Similar sized, globular, electron dense cytoplasmic inclusions contained phosphorus and calcium. We suggest that previous studies assigning calcium and phosphorus to gamma particles may have been based on confusion of these two types of cytoplasmic inclusions.
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  • 13
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    Planta 160 (1984), S. 12-20 
    ISSN: 1432-2048
    Keywords: Calcium ; Calmodulin ; Germination (spore) ; Onoclea ; Phytochrome and Ca2+ ; Pteridophyta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome is confirmed to be the photoreceptor pigment in the germination response of Onoclea sensibilis L. by demonstrating red-far-red (R-FR) photoreversibility. External Ca2+ is required for this response with a threshold at a submicromolar concentration. Ethylene glycol-bis(β-amino-ethyl ether)-N,N,N′,N′-tetraacetic acid, La3+ and Co2+ reversibly inhibit germination. Lanthanum only inhibits germination when applied before or during irradiation, indicating that the external Ca2+ requirement is transient, although in the absence of Ca2+ the R-stimulated system remains maximally poised to accept the ion for over 4 h after irradiation. The ability to respond to Ca2+ 4.1 h after R-irradiation is not reversed by FR-irradiation, indicating that Ca2+ transport has been uncoupled from phytochrome. Barium and Sr2+, but not Mg2+ can substitute for Ca2+. Artificially increasing the concentration of intracellular free Ca2+ with the ionophore A 23187 stimulates germination in the dark. The Ca2+-calmodulin antagonists, trifluoperizine and chlorpromazine, reversibly inhibit germination. Calcium is required in phytochrome-mediated fern spore germination; it may be acting as a second messenger.
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  • 14
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    Theoretical and applied genetics 68 (1984), S. 335-345 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Developmental genetics ; Cytoplasmic inheritance ; Genetic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-dimensional gel electrophoresis of denaturated proteins were performed at five developmental stages or organs (hereafter referred to as stage-organs) on two wheat lines with four different cytoplasms. Five hundred and fifty to 712 reproducible spots were scored depending on the stage-organ. Each stage-organ is unambiguously characterized and several types of control of protein quantity are recorded. Post-translational modifications are hypothetized and may sometimes be stagespecific. Two cytoplasmic patterns are found: one for the euplasmic lines with Triticum aestivum cytoplasm and one for the alloplasmic lines with Aegilops juvenalis, Ae. ventricosa and Ae. kotschyi cytoplasms. Cytoplasmic variation is observed for 28 spots showing position difference, all of which are probably products of the LS gene, and for four spots showing differences for regulation of protein quantity. Nuclear variation between ‘Chinese Spring’ and ‘Selkirk’ is found for 20 allelic differences and for 20 regulatory systems, the latter number being probably underestimated.
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  • 15
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Electrophoresis ; Hybridological analysis ; Genetic nomenclature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inheritance of gliadin components in winter wheat has been studied by one-dimensional polyacrylamide gel electrophoresis. Single F2 grains from 36 intervarietal hybrid combinations have been analysed. The genetic analysis has revealed blocks, including 1–6 gliadin components, which are inherited as individual mendelian traits. About 80 variants of blocks have been detected. On the basis of the allelism test they are grouped into 6 series in accordance with the number of known gliadin-coding loci located on chromosomes of the homoeologous groups 1 and 6. Each series includes 8–18 blocks controlled by different alleles of one gliadin-coding locus. Blocks of components have been confirmed to be inherited codominantly in accordance to the gene dose in the triploid endosperm. The highest similarity between members of one series is observed in groups of blocks controlled by chromosomes ID and 6D. On the contrary, many blocks controlled by chromosomes 1A and 1B have no bands in common. The presented catalogue of blocks of components may be used to make up gliadin genetic formulae and to compare electrophoregrams obtained by different authors. Blocks of gliadin components are suitable genetic markers for use in revealing and studying heterogeneity of wheat varieties, in tracing their origin, in identifying recombinations, translocations and substitutions of the genetic material and in solving many other problems of the origin, evolution and selection of hexaploid wheat.
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  • 16
    ISSN: 1432-2242
    Keywords: Amphiplasty ; Ag-NOR ; Addition lines ; Nucleolar organizer ; Agropyron elongatum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleolar organizer activity of the Agropyron elongatum, its amphiploid with hexaploid wheat (Triticum aestivum) and the chromosome addition lines is analyzed by the silver-staining procedure. Four Ag-NORs are observed in A. elongatum corresponding to the chromosomes 6E and 7E. In the amphiploid T. aestivum — A. elongatum, eight Ag-NORs are observed which corresponds the wheat chromosomes 1B and 6B and to the elongatum chromosomes 6E and 7E. Thus, there is codominance in the nucleolar organizer activity of the chromosomes of the two species. However, a partial amphiplasty is detected since less than 8 Ag-NORs (7 up to 4) are observed in some metaphase cells; the chromosomes 6E and 7E are occasionally suppressed by wheat chromosomes. This conclusion is confirmed by the behaviour of the addition lines since only in those corresponding to the chromosomes 6E and 7E are the elongatum chromosomes nucleolar active although occasionally they can be suppressed by wheat chromosomes.
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  • 17
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    Theoretical and applied genetics 69 (1984), S. 193-198 
    ISSN: 1432-2242
    Keywords: C-banding ; Heterochromatin ; Meiosis ; Common wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The meiotic identification of nine pairs of chromosomes at metaphase I of meiosis of Triticum aestivum (B genome, 4A and 7A) has been achieved using a Giemsa C-banding technique. As a result, the analysis of the pairing of each chromosome arm in disomic and monosomic intervarietal hybrids between ‘Chinese Spring’ and the Spanish cultivar ‘Pané 247’ could be carried out. Differences in the chiasmata frequencies per chromosome arm cannot be explained on the basis of relative arm lengths only. Possible effects of arm-to-arm heterochromatic differences on meiotic pairing are discussed.
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  • 18
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Two-dimensional electrophoresis ; Hybridological analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Blocks of gliadin components found both in a number of varieties and in single F2 grains of winter wheat intervarietal hybrids have been studied by two-dimensional electrophoresis combining electrophoresis in acidic aluminium-lactate buffer (pH3.1) and SDS-electrophoresis. Gliadin components (spots) have been shown to be inherited as linked groups (blocks), codominantly and in accordance with a gene dosage in triploid endosperm. Blocks include components differing in their electrophoretic mobility and molecular weight. Some allelic variants of blocks differ only in presence of few additional components or in the electrophoretic mobility of components with similar molecular weights; other variants may contain no similar components. Apparently, in the course of evolution, mutations in individual genes of gliadin-coding loci and processes changing the number of expressing genes and the sizes of their structural part occurred.
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  • 19
    ISSN: 1432-0878
    Keywords: Ionophore A-23187 ; Calcium ; Exocrine pancreas ; Secretion ; Zymogen granule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rat-pancreas lobules were incubated with the ionophore A-23187 in the presence of Ca2+. After 90 min, some of the acini were partially or almost completely depleted of their zymogen granules while others had the appearance of resting acini. With few exceptions, the cells of a given acinus were degranulated to a comparable level. Slight dispersion of the zymogen granules was noticed in cells incubated in a Ca2+-free medium containing EGTA with or without A-23187. In the presence of Ca2+ the secretory response obtained with the ionophore was comparable to that observed with 10-5M urecholine. The results obtained provide cytological evidence that the secretory response is only partially determined at the membrane-receptor level and that other mechanisms intervene between cytosol Ca2+ increase and exocytosis.
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  • 20
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    Cell & tissue research 236 (1984), S. 237-244 
    ISSN: 1432-0878
    Keywords: Aster formation ; Calcium ; Endoplasmic reticulum ; Hypertonic stress ; Parthenogenetic stimulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sea urchin eggs exposed to a continuous hypertonic treatment rapidly form many concentric whorls of endoplasmic reticulum (ER) during the pre-activation period of the parthenogenetic development. These whorls, however, are only a temporary configurational alteration of ER which begin to break up just prior to egg activation. The conversion back to normal vesicles and lamellae occurs not only concurrently with the appearance of early cytastral areas, but also frequently in close association with the formation of these membranous areas. It is revealed here that membrane elements from disrupting whorls may become incorporated into adjacent, developing clear areas, early cytastral areas, and that this ER constitutes an initial major source of membranes for these early astral areas. Having previously suggested that the actual formation of ER whorls occurs in direct response to released intracellular calcium in hypertonic stressed eggs, the new findings, along with other related data and correlations, further suggest that whorl disruption and the formation of associated astral areas can be correlated with a corresponding decrease in the concentration of this released calcium in the cytoplasm.
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  • 21
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    Plant systematics and evolution 145 (1984), S. 1-13 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticum aestivum ; Aegilops longissima ; Cytogenetics ; homoeologous relationships ; chromosome substitutions ; translocations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationships of three wheat-Aegilops longissima chromosome addition lines A, C, and D with homoeologous wheat chromosomes were studied in PMC meiosis. Substitutions of alien chromosome A for wheat chromosome 6 B, chromosome C for 1 B and chromosome D for 4 B were obtained. The production of 4 BS/C and 7 BS/D chromosome translocations indicated cytogenetic relationships of C partially to homoeologous wheat chromosomes of group 1 and 4, and D partially to homoeologous wheat chromosomes of group 4 and 7.
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  • 22
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    Protoplasma 120 (1984), S. 209-215 
    ISSN: 1615-6102
    Keywords: Fusion ; Calcium ; Protoplast ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rather than selecting for a chemical fusogen one can select for a fusogenic plant membrane (i.e., one that will fuse readily). Wild carrot suspension culture cells can be grown under conditions which cause the released protoplasts to have a high potential to fuse. Protoplast fusion is enhanced by calcium and inhibited by EGTA. When 10mM calcium (pH6.0) is added, fusion percentages of 60% are common. The mild fusion treatment appears to have no effect on callus regeneration and differentiation.
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  • 23
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    Protoplasma 121 (1984), S. 17-24 
    ISSN: 1615-6102
    Keywords: Chilling-sensitivity ; Calcium ; Chlortetracyline ; A 23187 ; Lycopersicon ; Digitalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have investigated the possibility that the rapid low temperature effects upon cyclosis and subcelluar structure might be due to a breakdown in compartmentation of intracellular calcium, leading to an increase in cytoplasmic Ca2+. Changes in fluorescence of chlortetracycline (CTC), a probe for membrane-bound Ca2+ were monitored in the corners of individual trichome cells (effective spot size ca. 800 square microns) with the aid of a Zeiss epifluorescence microscope linked to a Zeiss Zonax analyzing system. A consistent decrease in signal was observed as cells of chilling-sensitiveLycopersicon esculentum Mill. (cv.Ace) were cooled below their threshold temperature for chilling sensitivity. On rewarming, as the temperature rose above the chilling threshold, there was an increase in fluorescent signal. In contrast, trichomes ofDigitalis purpurea (chilling-resistant) showed no such changes. The uncoupling agent, CCCP, and the Ca2+-chelator, EGTA, induced marked decreases in the fluorescent signal in cells from both species. A more direct approach to testing the hypothesis was to examine the effect of modulating cytoplasmic Ca2+ with the aid of the Ca2+ -ionophore A 23187 and a Ca2+ concentration series in EGTA buffer. Above 10−8 M external free Ca2+, streaming began to be inhibited, full inhibition occurring at 5 x 10−6M Ca2+. The strand structure started to disappear when the Ca2+ rose above 10−7M. Disappearance of strands was accompanied by an increase in the number of cells with vesiculated cytoplasm, an effect analogous to that of chilling temperatures on these cells. The phenothiazines, trifluoperazine and chlorpromazine (10−5M) had similar effects. However but such effects were not seen with R 24571 or N(6-aminohexyl)5-chloro-1-napthalenesulfonamide (W 7) until concentrations were reached that orders of magnitude above their IC50 for calmodulin.
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  • 24
    ISSN: 1615-6102
    Keywords: Calcium ; Contraction ; Discophrya ; Ionophore A23187 ; Ruthenium red ; Tentacles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The tentacles of the suctorian protozoonDiscophrya collini are stimulated to contract by externally applied Ca2+. The role of extracellular Ca2+ in tentacle contraction was studied by monitoring45Ca2+ uptake, using ionophore A23187 to facilitate membrane transport of calcium and ruthenium red (RR) as an inhibitor of transport. The degree of tentacle retraction was dependent upon external Ca2+ concentration and studies with45Ca2+ using scintillation counting indicated a linear relationship between external Ca2+ concentration and Ca2+ uptake. Uptake of Ca2+ was enhanced in the presence of the ionophore while RR caused little inhibition.45Ca2+ uptake was only partially inhibited by RR when cells were subjected to a Ca2+, ionophore and RR mixture. Grain counts from light microscope autoradiographs after treatment of cells with45Ca2+/ionophore,45Ca2+/RR or45Ca2+ alone showed heavy, light and intermediate labelling respectively. In all instances the grains were evenly distributed within the cell. These observations are interpreted as supporting the suggestion that the ionophore enhances both the uptake of extracellular Ca2+ and release of Ca2+from an internal source, while the RR could only partially prevent movement of Ca2+ through the plasma mebrane. A model is presented suggesting that tentacle retraction is mediated by cytosolic Ca2+ levels which are determined by the fluxing of Ca2+ across the plasma membrane and the membrane of elongate dense bodies which act as internal Ca2+ reservoirs.
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  • 25
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; grain yield ; phosphate response ; height ; harvest index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The response to phosphate fertilizer by modern wheat genotypes was examined in the field under natural rainfall in three seasons. Models were developed which show that grain yield was positively correlated with biological yield and harvest index. In one of the seasons, which was relatively dry, shorter statured wheats gave higher yields at each level of applied phosphate. Higher levels of phosphate tended to offset the reduction in yield associated with late heading and the importance of biological yield on grain yield. The genotypes which produced the largest number of grains m-2 produced the highest yields. Implications for plant breeding programs are discussed.
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  • 26
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    Euphytica 33 (1984), S. 107-112 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; male sterility ; meiotic analyses ; gene mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetic and cytogenetic studies were done on a male sterile mutant of the wheat variety ‘Probus’. Association of the 4A chromosome carrying the ms gene was studied in the F1 of the male sterile ‘Probus’ with ‘Chinese Spring’ ditelo 4AS, with ‘Transec’ and with line ‘T4AS-DRS’ respectively. The presumption that the genetic male sterility of the mutant was due to a terminal deletion of the short arm of chromosome 4A could be confirmed. Linkage studies showed that the ms gene was at 17 map units from the dwarfing gene (Rht3) of ‘Minister dwarf’. This allows selection of short male sterile plants at the seedling stage.
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  • 27
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; glutenin ; high molecular-weight subunits of glutenin ; SDS-PAGE ; 1R(1B) substitution identification ; 1BL/1RS translocation identification ; bread-making quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The high-molecular-weight subunits of glutenin from wheat 1R(1B) substitution and 1BL/1RS translocation lines were fractionated by SDS-PAGE. Two new subunits denoted R1 and R2 were characterized in 1R(1B) substitution, but not in 1BL/1RS translocation lines. R1 and R2 were proved to be rye proteins by 2d electrophoresis (NEPHGE x SDS-PAGE). In contrast to literature citations it was demonstrated that the cultivar Winnetou is a 1R(1B) substitution line and the cultivars Clement and Mildress both are 1BL/1RS translocation lines.
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  • 28
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; common wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; resistance ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three lines derived from the old ‘dirty’ Dutch land variety Gelderse Ris were resistant against race 66(70)EO(16) of yellow rust. It was found that this resistance was conditioned by one recessive gene provisionally coded yrGR.
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  • 29
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    Euphytica 33 (1984), S. 757-767 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; water stress ; photosynthesis ; stomatal resistance ; leaf water potential ; water efficiency ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The responses of six wheat genotypes to water stress were analysed. Soil moisture (H), leaf water potential (Ψw), photosynthesis (PN), stomatal resistance (rs) and transpiration (T) were measured during a water stress. The genotypes investigated differed in their stress avoidance (Ψw-H relationship) and their stress tolerance (PN-Ψw and rs-Ψw relationships). The most important differences observed concern the mechanisms of tolerance at low leaf water potential: two varieties, Haurani 27 and Baalback, can then maintain a high photosynthetic activity. These observations are in agreement with the drought resistance characteristics already known for these genotypes. Possible applications to wheat breeding are considered
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  • 30
    ISSN: 1573-5060
    Keywords: Triticum dicoccoides ; wild emmer ; Triticum aestivum ; bread wheat ; nitrogen uptake ; plant nitrogen distribution ; translocation efficiency ; harvest index ; domestication ; evolution ; yield components ; grain protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences were found in total nitrogen uptake and its pattern of distribution in the main tiller amongst five lines of wild tetraploid wheat (Triticum turgidum dicoccoides) and between it and two hexaploid wheats (Triticum aestivum) under low (48 ppm) and higher (240 ppm) levels of soil nitrogen. Under the low soil nitrogen level the hexaploids had higher amounts of total nitrogen in the main tiller than the dicoccoides lines, but under the higher soil nitrogen level, three of the dicoccoides lines had significantly (P〈0.01) higher, and the other two lines, similar amounts as the hexaploids. The total amount of grain nitrogen in the hexaploids was significantly (P〈0.01) higher than the five dicoccoides under the low nitrogen soil level but under the higher level, two of the dicoccoides lines had similar amounts as one of the hexaploids (cv. Bencubbin) but significantly (P〈0.01) lower than the other (cv. Argentine IX). The efficiency of nitrogen translocation to the grain was significantly (P〈0.01) lower in a primitive, compared with four cereal forms of dicoccoides under both low and high levels of soil nitrogen. The cereal forms of dicoccoides, while similar in nitrogen translocation efficiency under low soil nitrogen as the lower translocation efficiency hexaploid (cv. Bencubbin), were significantly (P〈0.01) and substantially lower than it under the higher soil nitrogen level.
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  • 31
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    Plant and soil 76 (1984), S. 319-337 
    ISSN: 1573-5036
    Keywords: Aggregates ; Aluminium ; Bacterial mucilage ; Binding agents ; Calcium ; Cation bridges ; Complexing agents ; Dispersion ; Electron microscopy ; Electrophoretic mobility ; Fungal hyphae ; Glues Iron ; Management Periodate ; Polysaccharides ; Rhizosphere ; Roots ; Slaking
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The stability of pores and particles is essential for optimum growth of plants. Two categories of aggregates macro- (〉 250 μm) and micro- (〈250 μm) depend on organic matter for stability against disruptive forces caused by rapid wetting. Dispersion of clay particles from microaggregates is promoted by adsorption of complexing organic acids which increase the negative charge on clays. The acids are produced by plants, bacteria and fungi. However, the dispersibility of clay in microaggregates is offset by the binding action of polysaccharides, mainly mucilages produced by bacteria, but also by plant roots and fungal hyphae. The stability of microaggregates is also enhanced by multivalent cations which act as bridges between organic colloids and clays. Macroaggregates are enmeshed by plant roots, both living and decomposing, and are thus sensitive to management, and increase in number when grasses are grown and the soil is not disturbed. Lack of root growth,i.e. fallow, has the opposite effect. Various implications for management of soil structure are discussed.
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  • 32
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    Plant and soil 78 (1984), S. 367-379 
    ISSN: 1573-5036
    Keywords: Acidity ; Aluminium ; Calcium ; Manganese ; Nodulation ; pH Rhizobium ; Rhizosphere ; Root elongation ; Root hairs ; Trifolium repens ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of factors associated with soil acidity (low pH, low calcium, high aluminium and high manganese) on theTrifolium repens-Rhizobium trifolii symbiosis were investigted under laboratory conditions using an axenic solution-culture technique. 200 μM manganese increased root elongation in the range pH 4.3–5.5, but had no effect on root hair formation, the number of Rhizobium in the rhizosphere, or nodule formation. Root elongation and root hair formation were unaffected at pH 4.3 when 500 or 1000μM calcium was supplied, whereas multiplication of Rhizobium in the rhizosphere and nodulation were inhibited at pH 4.3 and 4.7.50–1000μM calcium had no effect either on the multiplication of Rhizobium in the range pH 4.3–5.5, or on nodule formation in the absence of aluminium. 50 μM aluminium inhibited, root elongation and root hair formation at pH 4.3 and 4.7; the effect on root elongation was reduced by increasing the calcium concentration from 50 to 1000μM. 50μM aluminium also inhibited Rhizobium multiplication in the rhizosphere and reduced nodule formation at pH 5.5 (at which aluminium precipitated out of solution), but root elongation and root hair formation were unaffected. These, effects of aluminium at pH 5.5 may explain the poor response to inoculation by white clover in acid mineral soils after liming.
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  • 33
    ISSN: 1573-5036
    Keywords: Calcium ; Cation de liaison ; Complexes organo-minéraux ; Minéralisation ; Stabilisation de la matière organique ; Structure ; Calcium ; Cation bridge ; Fixation of organic matter ; Mineralisation ; Organomineral compounds ; Structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary By gradually removing the exchangeable calcium held by the organo-mineral complex of an organic soil from the Jura mountains, the authors showed: a direct action of the microflora on the mineralisation of organic matter (Fig. 7) and on the generation of mineral nitrogen (Fig. 3); an indirect action by the mineralisation of organic matter on the destabilisation of the clay-humus complex. They therefore concluded that the reduction of calcium resulted in the destabilisation of the organo-mineral complex by H+, produced by the biodegradation of the most labile portion of the organic matter. The clay humus complex gradually dissociated. This process lead to the destruction of aggregates and organo-mineral complexes. The organic matter released became biodegradable due to the shortage of calcium.
    Notes: Résumé Les auteurs sont partis d'un complexe organo-minéral calcique provenant d'un sol humifère du Jura et ont provoqué une diminution progressive au cours du temps du taux de calcium échangeable. L'étude de l'évolution de divers paramètres, menée parallèlement, a permis d'analyser les effets directs et indirects de la baisse du calcium sur le complexe organo-minéral. Les résultats obtenus concernent: 1. les effets directs de l'action minéralisatrice de la microflore sur la dégradation de la matière organique (Fig. 7) et la production d'azote minéral (Fig. 3). 2. les effets indirects de cette minéralisation sur la déstabilisation du complexe argilo-humique (Fig. 8). Ils permettent d'affirmer qu'un complexe organo-minéral calcique évolue très rapidement quand l'apport d'ions calcium vient à diminuer ou à être supprimé. Les ions Ca2+ sont très vite déplacés et remplacés par des ions H+ produits par la biodégradation des fractions les plus labiles de la matière organique. Le complexe argile-calcium-humus est progressivement dissocié. Le processus conduit à une destruction des agrégats avec libération des argiles et de la matière organique qui devient à son tour biodégradable. l'effet stabilisateur du calcium ayant disparu.
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  • 34
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    Plant and soil 78 (1984), S. 441-444 
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis vartritici ; Hay-die ; Manganese deficiency ; Take-all ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The hypothesis that wheat plants deficient in managenese are predisposed to infection byGaeumannomyces graminis is outlined, and a test of the hypothesis in a soil system is reported. The results supported the hypothesis: wheat plants growing in managanese-deficient soil, although not showing foliar symptoms, were markedly more susceptible to infection; plant analysis confirmed the nutrient status of the plants. A review of the literature on take-all in wheat coupled with the results of our experiments suggests a reinterpretation of the etiology of this disease, since those edaphic factors which promote infection by this organism are those which also render managese unavailable to the host. Managenese nutrition is proposed as a common factor in many of the environmental conditions which influence the host-pathogen balance.
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  • 35
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    Plant and soil 79 (1984), S. 101-121 
    ISSN: 1573-5036
    Keywords: Base saturation ; Bio-climatic zones ; Calcium ; Clay accumulation ; Copper ; C:N ratio ; EDTA ; Forest soils ; Greece ; Iron ; Magnesium ; Manganese ; Nitrogen ; Phosphate ; Potassium ; Soil classification ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soils derived from a number of different parent materials (lithologies) and developed along a climatic gradient, manifested by the altitudinal succession of natural vegetation zones (Mediterranean, sub-Mediterranean, Mountainous and Pseudoalpine), were sampled throughout mainland Greece. In soils derived from siliceous parent materials low in clay, acidity increase and percent base saturation decreases from the Mediterranean to the Pseudoalpine vegetation zones. Clay illuviation is found mainly in soils developed in the Mediterranean and the sub-Mediterranean zones. No such changes are apparent in clayey soils rich in bases. Organic matter content of the mineral portion of the soil profile increases by a factor of 2 with a decrease in mean annual air temperature of about 10°C. The pattern of change in clay and soil organic matter content with climate is in relatively good agreement with soil development trends in the area, when soil profiles are named according to the FAO-Unesco soil map of the world. Concentrations of Ca and Mg decrease and those of total N, total and extractable P, K, Fe, Mn and Zn increase from the Mediterranean to the Mountainous zone. Within the same zone, however, concentrations of N, Ca, K, Fe, Mn and Zn decrease, but those of Mg, total and extractable P increase with soil depth. The concentrations of most macro- and micronutrients in the humic horizon are several times higher than those in the mineral portion of the soil profile due to biological enrichment.
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  • 36
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    Euphytica 33 (1984), S. 91-98 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; near-isogenic lines ; chromosome substitution lines ; vernalization ; photoperiod, ear emergence ; basic development rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies were made of days to ear emergence under the constant temperatures of 9, 14, 19 and 25°C and 16 h photoperiod in three sets of wheat lines each possessing genotypes differing for developmental responses. Days to ear emergence in three near-isogenic lines of the wheat cultivar Triple Dirk, which differed for vernalization response, increased as the strength of the response increased. At the four temperatures Triple Dirk D (Vrn 1 vrn 2) was not significantly different from normal Triple Dirk (Vrn 1 Vrn 2) but Triple Dirk B (vrn 1 Vrn 2) was significantly (P=0.01) later than normal Triple Dirk at each temperature. This indicates that the vrn 1 allele confers stronger vernalization response than vrn 2 over the range of temperatures (9–24°C). However, Triple Dirk C (vrn 1 vrn 2) failed to head after 120 days at each temperature indicating strong interaction between vrn 1 and vrn 2 with each other (and possibly the Triple Dirk back-ground) to give a much stronger vernalization response than predictions from additivity of their individual effects. The second set comprised the four Chinese Spring/Thatcher chromosome substitution lines CS/T 3B, 6B, 7B and 5D, plus Chinese Spring and Thatcher, and were grown in the unvernalized condition. CS/T 5D was similar in days to ear emergence as Chinese Spring at all four temperatures but the other three lines were earlier to ear emergence, particularly as the temperature increased. Days to ear emergence was fastest at 14°C in all lines, except CS/T 3B, in which it decreased progressively from 9 to 24°C. The third set of Chinese Spring and Thatcher and the homoeologous group 2 chromosomes of Thatcher substituted in Chinese Spring, the group which is considered to be involved in the control of photoperiod sensitivity. The three substitution lines responded differently to temperature compared with Chinese Spring and with each other, with chromosome 2D being the least, and chromosome 2B the most, responsive to temperature.
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  • 37
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    Euphytica 33 (1984), S. 123-132 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; durable resistance ; Puccinia striiformis ; yellow rust of wheat ; race-specific resistance ; race-non-specific resistance ; trangressive segregation ; stripe rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made between wheat varieties Joss Cambier, Nord Desprez and Maris Bilbo, all classified as susceptible to yellow rust in field tests, and between Cappelle Desprez and Maris Huntsman, both classified as moderately and durably resistant. Selection for resistance to yellow rust among the progeny was carried out using races of Puccinia striiformis able to overcome all the known race-specific components of resistance in both parents of each cross. Lines with greater resistance than in both parents were obtained from each cross, those with greatest resistance being obtained from the cross between the moderately resistant parents. Three lines selected for resistance from the cross of Joss Cambier with Nord Desprez and one from the cross of Cappelle Desprez with Maris Huntsman, together with the parents, were tested in the field with 12 races of P. striiformis. Nord Desprez possessed a previously undetected race-specific component. The selected lines also displayed race-specific resistance, some of which was clearly related to race-specificity of the parents, and a component of resistance, greater than in both parents, that was effective against all 12 races. The possible origin and potential durability of this transgressive level of resistance is discussed. It is suggested that such transgressive resistance is more likely to be durable if it is derived from parents that have shown durable resistance.
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  • 38
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    Euphytica 33 (1984), S. 227-232 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; selection ; inbreeding ; maternal inheritance ; kernel color
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Data are presented which support early generation selection for white kernel color in the progeny of red/white kernel wheat crosses which are segregating at 12 or more loci for yield (or any other trait of interest). The optimum generation for selecting white kernels is determined by the frequency of seeds produced with the potential to produce plants having desired quantitative alleles from both parents, and by the frequency of white kernels produced. The F2-produced seed (F3 embryo) is shown to be the optimum generation for selecting white kernels, given that 12 or more loci are segregating for a quantitative character such as yield. When the red parent is a 2 or 3 loci red genotype, selection among F4-produced seed for white kernel color may be desirable when 5 or fewer genes are segregating for the second trait. The results have direct application for all highly heritable, recessive, sporophytic traits.
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  • 39
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    Euphytica 33 (1984), S. 233-239 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; read wheat ; Triticum durum ; durum wheat ; Secale cereale ; rye ; inbred lines ; intergeneric crossability ; embryo differentiation ; plantlet recovery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary One hexaploid wheat cultivar (Triticum aestivem) and two tetraploid wheat lines (T. durum) were crossed with seventeen inbred lines of rye (Secale cereale). Seed set, degree of hybrid embryo differentiation at the time of excision for in vitro culture and recovery of amphihaploid plantlets from various embryo categories were studied. Degree of embryo differentiation was predominantly determined by maternal wheats, paternal rye genotypes appearing to be of minor importance. T. aestivum x rye hybrid embryos were superior to those produced from T. durum for degree of differentiation. The proportion of plantlets developing from differentiated embryos was high for all wheat parents, whereas undifferentiated embryos were mostly unsuitable for plantlet production. The results revealed that cross-incompatibility in hexaploid wheat x rye crosses was due to failure of fertilization, while in tetraploid wheat x rye crosses it was caused by lack of embryo differentiation. Correlation analyses showed that seed set provided a criterion to predict the amphihaploid plantlets to be expected from a particular wheat x rye combination.
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  • 40
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum turgidum dicoccoides ; wild emmer ; homologous recombination ; homoeologous recombination ; Ph-mutants ; high protein ; kernel weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat pentaploids were produced by hybridizing a high kernel weight (1000 grain wt=56 g), high protein (25.4%) line of wild tetraploid wheat (Triticum turgidum dicoccoides) as male parent, with the three hexaploids (T. aestivum) — normal Chinese Spring and its two homoeologous pairing mutants, ph 1b and ph 2. The pentaploids were crossed as female parents to the two commercial hexaploid cultivars Warigal and Barkaee and 42-chromosome stable plants selected from the F1 of the pentaploid x hexaploid crosses. Mean protein content of certain F3 lines from all six pentaploid x hexaploid crosses was significantly higher than Chinese Spring and the respective commercial hexaploid parent (p〈0.005) indicating high protein had been transferred from the tetraploid to the hexaploid level. Kernel weight amongst certain F3 lines of the three pentaploids x Barkaee was significantly (p〈0.0005) higher than either Chinese Spring or Barkaee, indicating the transfer also of high kernel weight from the tetraploid to the hexaploid level. However kernel weight was not significantly increased over Warigal in any F3 lines of its crosses with the three pentaploids. High levels of homoeologous chromosome pairing in the ph-mutant pentaploids, plus evidence for significant modification of the composition of high-molecular weight (HMW) glutenin subunits of grain protein in certain F3 derivatives of the ph-mutant pentaploid x hexaploid, crosses indicates that the ph-mutant-derived lines may possess novel (intergenome) genetic recombination, at least for high protein, and possibly kernel weight.
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  • 41
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    Euphytica 33 (1984), S. 489-495 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; multilines ; recurrent parents ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three multilines each of Kalyansona and PV 18 varieties of wheat were compared for their stability of yield and agronomic characters and disease resistance against the respective recurrent parents. The experiments were conducted for four years at nine locations. The multilines had more tillers and bolder seeds than the recurrent parents. There were, however, no differences for plant height, ear length and number of spikelets per spike. The genotypes x years x locations interaction was much more important than genotypes x years or genotypes x locations interaction as well as the main effects for genotypes. It is suggested that the number of test locations should be increased while decreasing the number of years. The stability parameters indicated that the multilines had a higher mean yield in the case of Kalyansona multilines whereas the yield of multilines of PV 18 was not significantly inferior to that of the recurrent parent. The regression coefficients were very close to unity except for KSML 3 (b=1.132). The deviations from regression were much larger for the multilines than for the recurrent parents. For rust resistance the multilines were superior over the varieties Kalyansona and PV 18.
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  • 42
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; breeding ; grain yield ; grain quality ; preharvest sprouting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hard white winter wheat (HWWW) occupies a very limited area of the USA, but its purported advantages suggest that its production in the major hard red winter wheat (HRWW) region may be feasible. Objectives of our investigations were to develop experimental HWWW lines that combined desirable attributes-grain yield, functional grain quality, and resistance to preharvest sprouting-in single genotypes for comparison with popular cultivars in the major US RHWW region. Forty-four lines from seven parental combinations were tested in randomized complete block designs at three Kansas locations during the 1981–82 and 1982–83 seasons. Agronomic traits, grain yield, grain quality, and preharvest sprouting were measured. Plant characteristics and grain yield were similar in the HWWW experimental lines and the HRWW check cultivar, Newton. Mean grain SDS-sedimentation value and grain protein content of most experimental lines equaled or exceeded that of the check. Dough mixing times frequently were shorter for the experimental lines than for the check cultivar, whereas loaf volumes were greater. Falling number usually was similar in all geneotypes, but α-amylase was higher in field-harvested grain of white lines than the check; both measures were more favorable than grain trade standards. We concluded that production of high yielding, high quality hard white winter wheat genotypes is feasible in the US ‘breakbasket’.
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  • 43
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    Plant and soil 80 (1984), S. 261-266 
    ISSN: 1573-5036
    Keywords: Azospirillum brasillense ; Wheat yield ; Triticum aestivum ; Responding genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty different wheat genotypes representing a wide genetic variability, were tested for their yield and yield components response to inoculation withAzospirillum brasilense, at two levels of N fertilization. Only two cultivars responded by a significant yield increase of 7.4 and 8.0 per cent — both at the higher N level. The response reflected an increase in the number of grains per plant added as additional spikes. The importance of the host plant genotype for a successful wheat-bacteria association is discussed.
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  • 44
    ISSN: 1573-5036
    Keywords: Lignin ; Manganese ; NO 3 − Phenols ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Managanese deficiency (〈 18 μg g−1 Mn) resulted in decreased levels of phenols in wheat shoots and decreased levels of lignins in both roots and shoots. These observed reductions in phenol contents was due largely to a decrease in the alkaline labile phenol component. Levels of nitrate supplied in solution influenced both phenol and lignin production; high nitrate levels (15 mM) resulted in a reduction in phenol and lignin in the shoot but stimulated lignin production in root tissue.
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  • 45
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    Plant and soil 82 (1984), S. 61-67 
    ISSN: 1573-5036
    Keywords: Calcium ; Glomus caledonium ; Glomus mosseae ; Lactuca sativa ; Vesicular-arbuscular mycorrhiza
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Infection of lettuce roots (Lactuca sativa L.) by the vesicular-arbuscular mycorrhizal fungiGlomus caledonium andGlomus mosseae was dependent on the amount of calcium (supplied as CaCl2·2H2O or CaSO4·2H2O) in the nutrient solution; those plants growing at low calcium concentrations being poorly infected.
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  • 46
    ISSN: 1573-5036
    Keywords: Aluminium toxicity ; Calcium ; Phosphate ; Trifolium ; Uptake ; Nutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hydroponic experiments were undertaken to examine the effect of increasing aluminium levels on the mineral nutrition and root morphology ofT. repens growing in nutrient solution. Toxicity symptoms appear between 27.8 and 47.5 μM Al3+ activity (148 to 297 μM total aluminium). The threshold level corresponding to a 10% reduction in leaf fresh weight is estimated to be approximately 20 μM Al3+ activity. The concentration of aluminium in the leaves of white clover increases exponentially with aluminium activity in the nutrient solution. The uptake of divalent cations was inhibited but aluminium enhanced potassium and nitrogen concentrations in both leaves and roots. At high pH (pH 6.0) the speciation of aluminium is controlled by the formation of solid aluminium phosphate and aluminium hydroxide except at the lowest aluminium level (37 μM) where 99.9 per cent is present as the DTPA complex. As the concentration of total aluminium increases, the percentage of Al-DTPA and soluble aluminium hydroxide decreases whilst solid Al(OH)3 increases rapidly to reach a maximum of 91.6 percent (of the total aluminium) in the 1180 μM aluminium treatment. At pH 4.5 the dominant forms of aluminium are free aluminium ion Al-DTPA, AlSO 4 + and AlOH2+. The roots of aluminium stressed plants showed symptoms typical of aluminium toxicity.
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  • 47
    ISSN: 1573-5036
    Keywords: Calcium ; Eucalyptus saligna ; Eucalyptus wandoo ; Foliar nutrients ; Magnesium ; Nitrogen ; Phosphorus ; Potassium ; Rehabilitation ; Seasonal trends
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seasonal changes in the foliar concentration of macronutrients (N, P, K, Ca and Mg) in sapling trees ofEucalyptus saligna Sm. andE. wandoo Blakely growing in rehabilitated bauxite mined areas in the Darling Range of Western Australia are described. Foliar N concentration decreased with age of the fully expanded leaf tissue. Leaf N concentrations were also high when rates of litter decomposition were expected to be high during the period of early spring. The greatest foliar N difference between trees growing in good soil conditions and those from poorer soil conditions also occurred during this period. Levels of P in leaves were highest in young developing leaves but once the leaves reached full size, no seasonal trend in P concentration was observed. Foliar K was lower during the winter and probably related to the period of maximum leaching by precipitation. High foliar K during summer, however, could be related to the role of K in lowering cellular water potential. Leaf Ca was highest during early sping. Low mobility of cellular Ca during the cool portion of the year was indicated. Foliar Mg showed a weak pattern of decreasing concentration with leaf age. The best season for sampling for these broadleafed evergreen species to provide information on plant nutrient status appears to be in spring.
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  • 48
    ISSN: 1573-5036
    Keywords: Azospirillum lipoferum ; Grain yield ; N-content ; Nitrogen fixation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Spring wheat (Triticum aestivum var. Arkas) was associated withAzospirillum lipoferum under greenhouse and field conditions of a temperate region. Controls were treated with autoclaved bacteria. The soils used were: sand, sandy loam, and a peat-clay mixture. In experiments run over a period of three years, there were increases in grain yield, N-yield of the grains, and 1000 grain weight. Depending from environmental conditions, increase changed from year to year, and within one given year. There was, however, no experiment without positive response to the inoculation. Highest grain yield increase (70%) was found on sand supplemented with P and K only, but up to 32% were also obtained on peat-clay soil containing 0.28% total N. Under greenhouse conditions, one third of technical N-fertilizer could be saved by bacterial activities. With high probability the effects observed have been at least partly due to bacterial N2-fixation, because the N-yield of the grains was increased (up to 33%), and the most pronounced response was found on sand without any N-fertilizer added.
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  • 49
    ISSN: 1573-5044
    Keywords: Triticum aestivum ; wide crosses ; tissue culture ; somaclonal variation ; Agropyron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Segments of young inflorescences of Triticum aestivum cv. Chinese Spring (CS), its F1 hybrids with Agropyron trachycaulum and A. scirpeum and backcross derivatives with A. yezoense, A. intermedium and A. junceum, and of a A. yezoense x T. aestivum cv. Wichita hybrid were cultured. Different parts of young spikelets of A. trachycaulum x CS F1 and A. yezoense x Wichita F1 's were also cultured. Percent callus induction was lower in wheat than in the wheat-Agropyron hybrids or backcross derivatives. Percent callus induction from different organs in both hybrids was in the descending order of whole spikelet, spikelet without glumes, rachis, and glumes. No plants could be regenerated from calli of wheat and backcross derivatives except those of CS x A. intermedium combination. Callus induction in hybrids varied from 54 to 84% and plant regeneration from 14 to 31%. The regenerants required no vernalization. Variants including one with top-dense spikes and another with elongated spikelets were recovered. Out of eight A. trachycaulm x CS hybrid regenerants, one had anthers and stigma as opposed to neutral flowers of the original hybrid.
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  • 50
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    Plant and soil 80 (1984), S. 181-190 
    ISSN: 1573-5036
    Keywords: Adsorption ; Calcium ; CEC ; Cell wall ; Clover ; Exchange ; Magnesium ; Model ; Potassium ; pH ; Root ; Rye-grass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The ion exchange properties of clover and rye-grass root cell walls were studied quantitatively. Three sets of experiments were performed: adsorption of Ca, Mg and K ions versus pH, adsorption versus cation concentration and exchange isotherms Ca−Mg and Mg−K. A model has been developed. It allows the satisfactory prediction of results (except for pH curves) with the adjustment of a minimum of parameters. The total charge (RT), on its own, accounts for the difference between the ion exchange properties of the clover and rye grass cell walls. The selectivities observed on root material are very different from those observed on soil exchange complexes. The decreasing affinities of cell walls for cations follow the sequence: Ca〉Mg≫K for cell walls. These differences of selectivity are much larger than those usually observed for soil exchange complexes.
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  • 51
    ISSN: 1573-5036
    Keywords: Azospirillum brasilense ; Wheat ; Triticum aestivum ; N-fertilizer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The yield response of a common spring wheat cultivar,Triticum aestivum, to inoculation withAzospirillum brasilense was studied at four levels of N fertilization. Plant yield increased due to the inoculation treatment only at medium and high levels of N fertilization, with a maximum yield increase of about 8.0 per cent at the highest level (approximately 1.0 g of pure N per plant). Yield increase was mostly due to an increase in the number of grains per spike, and at the highest level of fertilization, also due to a higher number of spikes per plant. At all N levels, the inoculation caused an increase of 0.5–1.4 per cent in the number of fertile spikelets per main spike. Grain protein percentage was unaffected by the inoculation, though significantly increase due to the fertilization treatments. The occurrence of maximum yield response at the highest N level, the response by early-determined yield components, i.e. spikelet number, and the unaffected grain protein content are in accord with the suggestion that the contribution ofAzospirillum brasilense to wheat yield is not through N2-fixation.
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  • 52
    ISSN: 1573-5036
    Keywords: Branch nutrients ; Calcium ; Eucalyptus saligna ; Eucalyptus wandoo ; Foliar nutrients ; Magnesium ; Nitrogen ; Phosphorus ; Potassium ; Rehabilitation ; Soil nutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The macronutrient variation within four 6 year oldEucalyptus saligna and four 5 year oldE. wandoo growing on rehabilitated bauxite pits was determined. Significant differences in mean nutrient concentrations were generally recorded between good soil condition sites and poor soil sites, between tree individuals, branch height, and plant organ type; but mean nutrient values were not different among canopy aspects. Fully expanded leaves of the current year provided the most uniform nutrient levels among the plant organs and showed major differences between sites with good soil nutrient conditions and those with poor conditions. Differences in foliar and branch levels of N, P, K, Ca and Mg, the variation between sites, canopy heights and plant organ types, and the use of foliar nutrient levels to indicate deficiencies are discussed.
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  • 53
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    Calcified tissue international 35 (1983), S. 107-110 
    ISSN: 1432-0827
    Keywords: Calcium ; Glucocorticoid ; Vitamin D ; Osteoporosis ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Eighty-eight adult male Sprague-Dawley rats were given a diet with either (a) 0.5% Ca and 0.6% P or (b) 0.01% Ca and 0.6% P. Osteopenia was created by adding prednisolone to the diet. The prophylactic effect of oral 1,25(OH)2D3 on the osteopenia was studied. It was found that prednisolone osteopenia in the rat was associated with defective Ca absorption. By giving an oral dose of 1,25(OH)2D3, it was possible to maintain normal Ca absorption during prednisolone treatment and to prevent the bone loss. No significant hypercalcemia or any kidney calcifications were seen. These results are in contrast to earlier findings, in which subcutaneous administration of 1,25(OH)2D3 failed to prevent prednisolone osteopenia because of its tendency to increase bone resorption.
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  • 54
    ISSN: 1432-0827
    Keywords: 31P NMR spectroscopy ; Phosphoprotein ; Dentin ; Calcium ; Inorganic orthophosphate ; Bovine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The single phosphoprotein of fetal calf dentin, having a molecular weight of approximately 94,000 and a phosphorus content of 8% (w/w), was examined by31P NMR spectroscopy. The single resonance at 3.7 ppm at pH 10 and its chemical shift during acid titration established the phosphomonoester nature of the organic phosphorus moiety. During titration of the phosphoprotein with CaCl2 in the presence of inorganic orthophosphate ions, line broadening for the orthophosphate resonance was both phosphoprotein- and calcium-dependent, indicating ternary complex formation. The data indicate that the phosphoprotein of fetal calf dentin binds both calcium and inorganic orthophosphate ions and therefore has the requisite physical chemical properties necessary for it to facilitate the heterogeneous nucleation of a Ca-PO4 solid phase from solution during tissue mineralization.
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  • 55
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    Planta 158 (1983), S. 320-328 
    ISSN: 1432-2048
    Keywords: Calcium ; Egeria ; Fluorescent probe ; Ions, group II ; Symplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hydrophyllic dyes fluorescein glutamic acid, fluorescein glutamylglutamic acid (F(Glu)2), fluorescein hexaglycine, fluorescein leucyldiglutamyl-leucine and 6-carboxyfluorescein are unable to pass the plasmalemma in leaves of E. densa. However, when injected into single cells the dye conjugates of molecular weight 665 dalton or less move freely from cell-to-cell. This intercellular movement presumably occurs via the plant symplast. Movement of F(Glu)2 from the injected cell occurs with greatly reduced frequency when Ca2+, Mg2+ or Sr2+ are injected into the cell immediately prior to the dye. The fraction of dye injections leading to movement declines with increasing group II ion concentration in the electrode tip, up to 10 mM. Sodium and K ions do not affect dye movement. When dye injection is delayed 30 min after Ca2+ injection, dye movement is no longer inhibited. Thus the cells recover from the Ca2+ injection, indicating that the ion does not cause major cell damage. Recovery from Mg2+ injection is not complete within 60 min. Treatment of leaves with chemicals expected to raise the concentration of free intracellular group II ions, notably the mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxyphenyl hydrazone, the inhibitor of mitochondrial Ca2+ uptake trifluralin, or the ionophore A23187 also inhibits dye movement, while the calmodulin inhibitor trifluoperazine does not. Cytoplasmic streaming is inhibited by Ca2+ or Mg2+ injection and by the metabolic inhibitors. However when streaming is stopped by cytochalasin B, dye movement is not inhibited. Hence steaming is not necessary for dye movement. Thus the cytoplasmic concentration of free group II ions may directly regulate the permeability of the plant symplast.
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  • 56
    ISSN: 1432-072X
    Keywords: Phototaxis ; Motility ; Flagellate ; Chlamydomonas ; Sodium azide ; Calcium ; A 23 187
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A fully automated and computerized method for simultaneous measurements of motility and phototaxis of unicellular flagellates is described. Both systems are directly coupled with a homocontinuous culture. The motility measuring apparatus is equipped with a video camera and recorder for simultaneous single cell behaviour studies. First results of studies on the effects of the phototaxis inhibitor sodium azide and the Ca2+ conducting ionophore A23 187 on motility and phototaxis of Chlamydomonas are reported and correlated with video observations. These results demonstrate that the described systems give informations of whether phototaxis or motility or both are inhibited by chemicals.
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  • 57
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    Theoretical and applied genetics 66 (1983), S. 101-109 
    ISSN: 1432-2242
    Keywords: Anther culture ; Culture temperature ; Induction frequency ; Pollen callus (plantlet) ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The response of anther culture to culture temperature was studied in detail using many varieties, F1 hybrids and pollen-derived lines of wheat (Triticum aestivum) as materials. The suitable culture temperature for inducing pollen callus (or embryoids) in wheat anther culture ranged from 26 °C to 30 °C, varying with genotypes. But for the great majority of wheat genotypes the suitable culture temperatures lay between 28 °C and 30°C. The most significant genotypic variation in the response to culture temperature was observed in the comparison between the culture at 33 °C for eight days followed by culture at 25 °C (or 26 °C) and the continuous culture at 25 °C (or 26 °C). This genotypic variation in the response to culture temperature is a heritable character which may be controlled by multiple genes. The effect of culture at 30 °C for eight days followed by culture at 26 °C was similar to, or in some cases, better than that of continuous culture at 28 °C, and the effect of culture at 32 °C for eight days followed by culture at 28 °C was similar to that of continuous culture at 30 °C. In the range from 26 °C to 32 °C, the overwhelming majority of pollen calli emerged before the 40th day after anther inoculation, and the higher the culture temperature, the earlier and more concentrated the emerging period of the pollen callus. The pollen callus obtained at high temperatures above 28 °C should be transferred in time onto the regeneration medium at 25°–27°C to induce shoots.
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  • 58
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    Theoretical and applied genetics 64 (1983), S. 255-258 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Malate dehydrogenase ; Chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The malate dehydrogenase (E.C. no 1.1.1. 37) of Triticum aestivum L. cv. Chinese Spring, shows two activity zones. The results obtained support the hypothesis that the malate dehydrogenase isozymes of zone II are dimers composed of the six possible combinations of subunits coded by triplicate genes located in the long arms of chromosomes of the homoeologous group 1.
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  • 59
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    Theoretical and applied genetics 66 (1983), S. 111-121 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron ; Intergeneric hybrids ; Embryo culture ; Chromosome pairing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids of Triticum aestivum (2n=42,AABBDD) with Agropyron ciliare (2n= 28,SSYY), A. trachycaulum (2n=28,SSHH), A. yezoense (2n=28,SSYY) and A. scirpeum (2n=28) are reported for the first time. F1 hybrids of T. aestivum were also produced with A. intermedium (2n=42,E1E1E2E2Z1Z1) and A. junceum (2n=14,JuJu). All wheat-Agropyron hybrids were obtained by embryo rescue technique. Cultivars and reciprocal crosses differed for seed set, seed development and F1 plant production. The F1 hybrids were sterile. Attempts to obtain amphiploids were unsuccessful. However, backcross derivatives were obtained with wheat as the recurrent parent. The level of chromosome pairing in A. trachycaulum x wheat, A. yezoense x wheat and wheat x A. junceum hybrids provided no evidence of homologous or homoeologous pairing. Mean pairing frequencies in A. ciliare x wheat, wheat x A. scirpeum and wheat x A. intermedium hybrids indicated homoeologous or autosyndetic pairing. Ph gene was more effective in regulating homoeologous pairing in A. yezoense x wheat hybrids than in A. ciliare x wheat hybrid. Chromosome pairing data of BC1 derivatives indicated that either some of the wheat chromosomes were eliminated or Agropyron chromosomes caused reduced pairing of wheat homologues.
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  • 60
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    Theoretical and applied genetics 65 (1983), S. 41-46 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Protein ; Mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. Four hard red winter wheat genotypes — KS644 (‘Triumph// Concho/Triumph’), ‘Kaw’, ‘Parker’, and ‘Shawnee’ — were treated with 0.40 M ethyl methanesulfonate (EMS). Advanced lines (M8-M10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. Increased grain protein content was generally associated with decreased grain yield and kernel weight, but some high-protein mutant lines had yields or kernel weights similar to those of original genotypes. Changes in height and lodging induced by EMS were generally favorable, most mutants being shorter and lodging less than controls, but blooming date was generally delayed, a deleterious change. One line also changed from resistant to segregating for wheat soil-borne mosaic virus. Mutant lines might be utilized in cross-breeding programs, particularly if negative pleiotropic effects and linkages are absent.
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  • 61
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    Theoretical and applied genetics 64 (1983), S. 97-101 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total endosperm protein subunits, extracted from the common wheat cultivar Chinese Spring and from some of its aneuploid lines, were fractionated according to their molecular weight (MW) in an improved high resolution one-dimensional sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). The resolution obtained by this method and, in particular, that of the high molecular weight (HMW) glutenin and gliadin subunits approached that of a previous report in which two-dimensional fractionation system based on charge and MW was used. In the cultivar Chinese Spring, 21 discrete protein bands were resolved and the chromosomes controlling many of them were either reconfirmed, or, in some cases, established. The advantages of this high resolution SDS PAGE technique are discussed.
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  • 62
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    Theoretical and applied genetics 67 (1983), S. 53-58 
    ISSN: 1432-2242
    Keywords: Aegilops umbellulata ; Genetics ; Lectin ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Each of the three genomes in hexaploid wheat controls the expression of a specific lectin in the embryo. The chromosomes which control their synthesis were determined using nullisomic-tetrasomic and inter-varietal chromosome substitution lines of ‘Chinese Spring’. All three wheat lectins were shown to be controlled by the homoeologous group 1 chromosomes. Using ditelosomic lines of ‘Chinese Spring’ the lectin genes could be localized on the long arms of chromosomes 1A and 1D. Inter-specific addition and substitution lines of Aegilops umbellulata chromosomes to ‘Chinese Spring’ indicated that chromosome 1U, which is homoeologous to the group 1 chromosomes of wheat, controls lectin synthesis.
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  • 63
    ISSN: 1432-2242
    Keywords: HMW glutenin subunit genes ; cDNA clones ; Tandem DNA repeats ; Chromosomal location ; Gene copy number ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA clones encoding wheat HMW glutenin subunits have been isolated from a cDNA bank made to poly A+ RNA from developing wheat endosperm var. Chinese Spring. One such clone, pTag 1290, has enabled us to identify the HMW glutenin mRNA species. The DNA sequence of this clone has been partially determined and it contains several tandem DNA repeats. The sequence is discussed in relation to the generation of the HMW glutenin subunit gene family. Analysis of the organization of the HMW glutenin sequences in the wheat genome revealed that the genes encoding HMW glutenin subunits exist in low copy number and are located on the long arm of each of the homoeologous group 1 chromosomes.
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  • 64
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. timopheevii ; Wheat ; Photoperiod ; Vernalization ; Male sterility ; Alloplasmic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies were conducted to determine the influence of the male sterility-inducing cytoplasm of Triticum timopheevii (Zhuk.) Zhuk. on response of several common winter wheat (T. aestivum L.) nuclear genotypes to photoperiod and vernalization. Comparative studies of cytoplasmic substitution lines provide information on the role of the cytoplasmic genetic mechanism in growth and development. In the case of cytoplasmic male sterility-based hybrid production systems, ubiquity of sterility-inducing cytoplasm in derived hybrids warrants thorough characterization of its influence on plant phenotype. Factorial combinations of cytoplasm (T. timopheevii and T. aestivum), nuclear genotype, and photoperiod or vernalization treatments were evaluated under hydroponic conditions in controlled environment chambers. Interaction of cytoplasm, photoperiod, and nuclear genotype was significant in one or more experiments for days to anthesis and potential spikelet number, and interaction of cytoplasm, vernalization, and nuclear genotype was significant for days to spike emergence. Long day length was associated with increased percentage seed set in one study, but interactions of photoperiod and cytoplasm were not detected for percentage seed set. Interactions involving cytoplasm and photoperiod or vernalization were interpreted as evidence of the existence of genetic factors in cytoplsam of T. timopheevii which alter photoperiod or vernalization responses of alloplasmic plants relative to responses exhibited by euplasmic plants. Since photoperiod and vernalization responses are critical to adaptation, T. timopheevii cytoplasm can alter adaptability of T. aestivum. The specific effect would be nuclear genotype dependent, and does not appear to be of a magnitude greater than that induced by nuclear genetic variability at loci conditioning photoperiod or vernalization responses or other adaptation-determining characteristics. Normal multilocation/year testing of alloplasmic hybrids should therefore adequately identify zones of adaptation.
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  • 65
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    Theoretical and applied genetics 66 (1983), S. 77-86 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins ; Glutenins ; Gliadins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endosperm protein subunits of 109 primitive and modern lines of hexaploid wheat, Triticum aestivum L. em. Thell., were fractionated by one-dimensional, high resolution, sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). A wide range of both qualitative and quantitative variation was observed in the fractions of the high molecular weight (HMW) glutenin and gliadin subunits of the different lines. The qualitative variation was expressed in the number of subunits per fraction and in their molecular weight, as determined by the differential rate of migration. The quantitative variation was expressed in the differential staining intensity of several subunits. The widest variation was detected in the HMW glutenin and gliadin subunits controlled by chromosome 1B while a much smaller variation was observed in those subunits controlled by chromosome 1A and further smaller variation in the subunits controlled by 1D. Only a small number of subunits in both fractions was found to be controlled by chromosome 1A indicating that diploidization of endosperm protein genes in common wheat has been non-random. The genetic and evolutionary implications of these findings are discussed.
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    Theoretical and applied genetics 66 (1983), S. 1-7 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Aegilops species ; Alloplasmic lines ; 2D gel electrophoresis ; Cytoplasmic inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
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    Protoplasma 115 (1983), S. 11-17 
    ISSN: 1615-6102
    Keywords: Pollen tubes ; Calcium ; Tip extension ; Vesicle fusion ; Microfilaments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pollen tube growth is shown to be inhibited by both suboptimal (〈10−4 M) and supraoptimal (〉10−3 M) Ca2+ ion concentrations. Tip extension rates are independent of Ca2+ ion concentration over the optimal range. Changes in the structure of the tips of pollen tubes after transfer to inhibitory Ca2+ ion conditions provide evidence in support of our proposed mechanism of pollen tube tip growth (J. theor. Biol.98, 15–20, 1982). It is clearly demonstrated that tip extension is controlled, not only by the rate of vesicle fusion, but also by the state of plasticity of the tip; these processes appear to be sensitive to changes in Ca2+ ion concentration.
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    Protoplasma 115 (1983), S. 104-113 
    ISSN: 1615-6102
    Keywords: Cereals ; Embryo culture ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell proliferation from the mature embryo of wheat occurs on a defined medium in the presence of 2,4-D. Unorganized growth is observed when the 2,4-D concentration is equal to or greater than 2 mg/l, but increasing levels of 2,4-D inhibit cell division. Cell divisions begin after 4 days in culture from parenchyma cells within and near the procambial tissues of the embryo axis. By day 8 continuous meristematic zones are formed in association with vascular tissues, and DNA synthesis and cell divisions are distributed throughout these zones. No morphological evidence exists to show that these zones consist of proliferating root primordia, which are formed only after the level of 2,4-D falls below some critical concentration. When the concentration of 2,4-D is lowered, the meristematic zones first become dissected and then give rise to many root meristems.
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  • 69
    ISSN: 1615-6102
    Keywords: Calcium ; Contraction ; Discophrya ; Ionophore A 23187 ; Ruthenium red ; Tentacle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The suctorian protozoonDiscophrya collini has contractile tentacles with microfilaments and a central microtubule-lined canal (axoneme). The role of calcium fluxing in tentacle contraction has been investigated using the Ca2+ ionophore A 23187 and ruthenium red (RR), a known inhibitor of certain Ca2+ membrane transport events. Treatment with CaCl2 alone caused tentacle contraction with a threshold at 5 × 10−3 M CaCl2 and a maximum at 5 × 10−2 M CaCl2 with contraction to 32.8% of the original length. In the presence of 5 μM ionophore A 23187 the threshold was lowered to 5 × 10−6 M CaCl2 with a maximum to 19.6% original length at 5 × 10−2 M CaCl2. A 23187 alone induced contraction with a threshold of 3.0 μM and a maximum to 30.5% original length at 10 μM A 23187. Treatment with RR alone had little effect on tentacle length. However, a 10 μM A 23187-induced contraction was partially counteracted by the simultaneous application of RR with a threshold at 2 μM RR and a maximum at 8 μM RR. Removal of the ionophore after induced tentacle contraction resulted in partial re-extension, which was inhibited by RR. Ultrastructural observations indicated that the ionophore and CaCl2-induced contraction processes are indistinguishable. The CaCl2/ionophore treatments led to axonome disruption, interpreted as a consequence of supranormal levels of intracellular Ca2+. X-ray microanalysis of cytoplasmic membrane-bound elongate dense bodies (EDB) showed a high level of Ca2+ in CaCl2-treated cells, little Ca2+ in ionophore-treated cells and intermediate levels in the RR-treated, ionophore/RR-treated and untreated control cells. It is suggested that A 23187 enhances both the uptake of extracellular Ca2+ and the release of Ca2+ from the EDB, the latter being counteracted by RR. These observations support the proposal that the EDB act as Ca2+ reservoirs, and that their Ca2+ fluxing moderates cytosolic Ca2+ levels which mediate a Ca2+-dependent tentacle contraction mechanism.
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  • 70
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    Protoplasma 117 (1983), S. 226-232 
    ISSN: 1615-6102
    Keywords: Calcium ; Calmodulin ; Chlorotetracycline ; Microfluorometry ; Phenothiazine ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pear (Pyrus communis L.) pollen was germinated and grown in hanging drop cultures containing phenothiazine drugs, trifluoperazine and chlorpromazine, potent inhibitors of the Ca2+-calmodulin complex. Responses for the two drugs were similar: at 1.0–2 10.0 ΜM pollen germination and tube growth were inhibited. Inhibition of tube growth was not uniform; at 10.0 ΜM growth of about half of those tubes which had germinated was inhibited while the remaining half of the population grew normally. This bimodal distribution of tube growth was noted, but to a lesser extent, at lower concentrations of the drugs as well. Microfluorometric analysis of membrane calcium using chlorotetracycline, a fluorescent chelate probe for membrane calcium, revealed that upon pollen grain hydration there was a sharp decrease in the concentration of calcium associated with membranes and that membrane calcium became localized primarily at the periphery of the newly hydrated pollen grain. Prior to germination there was a reloading of calcium onto membranes in the region of the germination aperture through which the pollen tube would emerge. The release of Ca2+ from membrane sites upon hydration was partially inhibited by treatment with 10 ΜM trifluoperazine. Distribution of membrane calcium in populations of the inhibitor-treated pollen grains was not bimodal however; approximately half of the population had CTC-fluorescence emissions exceeding the maximum value found in the control population. These results suggest that there is a maximum concentration of membrane-associated calcium consistent with normal pollen germination and tube growth and that phenothiazines interfere with the unloading of Ca2+ from membrane sites.
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  • 71
    ISSN: 1573-5036
    Keywords: Calcium ; Nutrient stress ; Phosphorus ; Potassium ; Root growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Techniques developed to measure growth of tomatoes and beans under limiting amounts of either P, K or Ca in solution culture reveal differences among strains. Genetic analysis permits estimates of gene action for control of efficiency and the isolation of improved segregants. The genetic isolates have value in studying mechanisms contributing to uptake, transport and utilization processes.
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  • 72
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    Euphytica 32 (1983), S. 217-223 
    ISSN: 1573-5060
    Keywords: Hexaploid triticale ; Secale cereale ; rye ; Triticum aestivum ; wheat ; heterochromatin ; chromosome association
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the hexaploid triticale (× Triticosecale Wittmack) cultivar Rosner chromosome 2R lacks the prominent heterochromatic bands of both telomeres. This modified 2R chromosome is capable of pairing in a high frequency with wheat chromosomes. It is hypothesized that the accumulation of heterochromatin at the telomeres of rye chromosomes may have contributed to the isolation of the wheat and rye genera by inhibiting pairing between wheat and rye chromosomes.
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  • 73
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    Euphytica 32 (1983), S. 431-438 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Triticum aestivum ; wheat ; T. turgidum ; durum wheat ; X Triticosecale ; triticale ; salt tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Saline soils are typically very patchy in their salinity. The yield of crops growing on them is similarly patchy. This paper argues that because most of the yield from such soils comes from the least saline areas, the best breeding strategy for improving the overall yield of crops growing on them is to select for high yield on non-saline soils. This conclusion derives from comparing the effects that four different breeding goals, namely: (1) a 10% increase in yield on non-saline soils, (ii) a 20% increase in the threshold salinity that first reduces yield, (iii) a doubling of yield at an electrical conductivity of the saturation extract (ECe) of 20 dS/m and (iv) a combination of (i) and (iii), would have on total yield. The effects of achieving these goals in barley, common wheat, durum wheat and triticale in fields exhibiting different salinities are predicted from actual yields of these species grown on different salinities in the field.
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  • 74
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; kernel color ; protein content ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Utilization of high-protein hard red wheat germplasm in breeding high-protein hard white winter wheats for the U.S. Great Plains raised concern regarding possible genetic relationships between kernel color and protein content. Segregating F3 and F4 populations from reciprocal crosses and backcrosses involving high-protein hard red winter wheat cultivar Plainsman V and normal-protein hard white winter wheat line KS75216 were examined. Nonsignificant regression and correlation coefficients in the F3 generations of KS75216/Plainsman V, KS75216//KS75216/Plainsman V and Plainsman V//KS75216/Plainsman V indicated the absence of genetic relationships between kernel color and protein content. Therefore, despite the presence of genes for protein content and kernel color on the same chromosomes (3A, 3B and 3D), kernel color and protein content appeared as independent traits. A small but significant negative relationship between white kernel color and high protein in Plainsman V/KS75216 was attributable to the possible presence of alien genetic material in the parentage of Plainsman V. Chi-square tests indicated that Plainsman V is a mixture of genotypes for kernel color; most genotypes carry two dominant genes for red color and a few carry one or three. Genetic control of grain protein appeared to be complex. Partial dominance for high protein was indicated in the F3 generation but a generally continuous distribution and transgressive segregation also suggested other genes functioned additively. Heritability estimates by parent-offspring (F3-F4) regression were sufficiently high to ensure genetic progress in the selection of high-protein lines in the red x white wheat crosses. We concluded that development of high-protein cultivars is as feasible for white wheats as for red wheats.
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  • 75
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    Euphytica 32 (1983), S. 299-310 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; ear colour ; glume colour ; genetics ; linkage ; chromosomal location ; geographical distribution ; homeoallelic genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A summary of the brown ear character of bread wheat is presented. In most varieties this character is conditioned by a (semi)dominant gene identified as Rg on 1BS. This gene seems to be widespread, from the viewpoint of evolution, probably because it is an ‘old’ gene. There are no indications that the presence of the gene and hence the presence of a brown ear is advantageous or disadvantageous to the carrier wheat plant. Some linkage relations are described. More research is needed to establish whether all varieties with one gene for brown ear carry Rg, to investigate the varieties with a non-monogenic genetic system and to identify more associations between brown ear and other characters. It should also further be investigated whether within T. spelta another gene for brown ear is present and, if so, whether this gene is on chromosome 1AS and linked to Hgl, the gene for hairy glume.
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  • 76
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    Euphytica 32 (1983), S. 743-748 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; plant physiology ; plant breeding ; Australia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Major changes in the behaviour of the Australian wheat crop over the last one hundred years have been associated with three major gene groupings. The significance of major genes in monitoring response to vernalization, photoperiod and gibberellin, reveals a more optimistic future for breeding programmes where simple genetic and physiologic studies are integrated within those programmes. A revised classification of growth habit is presented in the appendix.
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  • 77
    ISSN: 1573-5036
    Keywords: Acetic acid ; Inhibition ; Wheat growth ; Tillering ; Root growth ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies were conducted to determine the effect of and duration of the effect of alliphatic acids on winter wheat (Triticum aestivum L.) seedling root growth, shoot growth, and tillering. Winter wheat seedlings grown in contact with unbuffered solutions of 2 mM or greater acetic or 0.5 mM or greater propionic or butyric acid for 3 days showed decreased root and, in general, shoot growth. Buffering the medium partially alleviated the problem. Removing the seedling from the acid medium and growing it in a nutrient medium resulted in accelerated root growth, compared with the control, while shoot growth was permanently inhibited during this study. Seedling wheat, grown with one root in contact with concentrations of acetic acid ranging from 0–16 mM and the other roots in aliphatic acid-free medium, grew at the same rate as the control. Seedling wheat grown for 3 days in 2 and 4 mM acetic acid medium showed a more rapid formation of the first stem tiller (T1) than did the control. Concentrations of 6 and 8 mM acetic acid appeared to delay T1 tiller formation through the first 18 days after germination, while only 10 mM acetic acid reduced T1 tiller formation by 30% 20 days after germination. The second stem tiller (T2) was not affected by previous exposure to acetic acid. The results of these laboratory studies indicate that short-term exposure of seedling winter wheat to short-chain aliphatic acids can result in permanent shoot and tiller damage and not in permanent root damage as previously thought. These results could explain the poor performance of no-till seeded winter wheat when growing through heavy crop residues that are producing shortchain aliphatic acids during decomposition.
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  • 78
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    Plant and soil 70 (1983), S. 77-93 
    ISSN: 1573-5036
    Keywords: Adsorption ; Calcium ; Calcium carbonate ; Cation Exchange Capacity ; Hydrogen ; Magnesium ; Moss ; pH ; Potassium ; Sodium ; Soil preference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Moss species with a different soil preference with respect to its acidity and carbonate content also differ in their cation exchange capacity (leaf, stem, rhizoid), the acidiphile-calcifuges having lower C.E.C. values than the neutrophile-calcicoles. The higher the C.E.C. value, the more the divalent cations calcium and magnesium tend to be adsorbed as compared to the monovalents sodium and potassium, from an equinormal tetracationic solution. The increase in divalent adsorption is mainly due to calcium. Considering this differential adsorption of calcium and in view of data in literature on membrane characteristics, it appears that among the studied cations calcium is an important factor in the observed soil preference through its presence in the cell wall adsorbed fraction and thus in the membrane environment.
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  • 79
    ISSN: 1573-5036
    Keywords: Alkali metals ; Alkaline earth metals ; Barium ; Calcium ; Ceasium ; Lithium ; Magnesium ; Potassium ; Rubidium ; Sodium ; Strontium ; Temperate forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Absorption and accumulation of alkali (Li, Na, K, Rb, Cs) and alkaline earth (Mg, Ca, Sr, Ba) metals were investigated as taxonomic characteristics (in 62 plant species). Leaf and soil samples were collected from 9 sites in temperature forest in Japan and the above mentioned elements were analyzed. Considerable differences were found among species in their ability to accumulate alkali and alkaline earth metals. Very high concentrations of Li (45 ppm, D.W.), K (37×103 ppm), Rb (159 ppm) and Cs (8.2 ppm) were detected inLastrea japonica which were about 412, 12, 27 and 6 times higher than those of the species with the lowest concentrations. Na content was high inAcer micranthum (358 ppm) which was 16 times higher than species with the lowest concentration. Other species containing high levels of alkali metals wereHydrangea macrophylla, Struthiopteris niponica, Clethra barbinervis. Mean discrimination ratio (D.R.) for all investigated plant species for Li, Na, Rb, and Cs to K were 1.7, 0.44, 0.9 and 1.8 respectively. High concentrations of alkaline earth metals Ca (36×103 ppm), Sr (345 ppm), and Ba (241 ppm) were found in the leaves ofHydrangea paniculata which were about 31, 84, and 72 times higher than those for the species with the lowest concentration. Mg was very high inStruthiopteris niponica (83×102 ppm). Other species with high concentrations of alkaline earth metals belonged to the genus Viburnum. Mean D.Rs. for Mg, Sr, and Bavs Ca were 1.0, 0.7 and 0.08. Principal component analysis of interrelationships between the mineral content in leaf tissues indicated that these elements could be classified into 2 groups with respect to their accumulation behavior in plants. The alkali metals K, Li, Rb, and Cs behaved similarly in their accumulation in leaves but Na behaved independently. Alkaline earth metals Ca, Mg, Sr, and Ba were also found to behave similarly in their accumulation. Factors scores of 1st and 2nd components revealed three groups of plant species: alkaliphilic, alkaline earthphilic, and neutral (non-accumulators).
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  • 80
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    Plant and soil 70 (1983), S. 309-316 
    ISSN: 1573-5036
    Keywords: Calcium ; Ionic interaction ; Magnesium ; Nutrient solution ; Oryza sativa L. ; Phosphorus ; Potassium ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ionic interactions may occur as cation-cation interactions, anion-anion interactions, or cation-anion interactions. Greater knowledge of this subject is warranted, due to the intensification of agriculture, in devising efficient fertilization systems. The present study was undertaken to elucidate the interaction among P, K, Ca and Mg absorption by the intact rice (Oryza sativa L.) plants from dilute solutions. The uptake of P was independent of Ca concentrations up-to 250 μM Ca but-there was a small decrease at the higher concentrations. Absorption of K and Mg was stimulated in the presence of Ca ions at low concentrations. But K and Mg absorption was decreased at higher Ca concentrations. Increasing concentrations of K in the nutrient solution depressed P and Ca uptake. Magnesium uptake was stimulated at lower concentrations of K, but at higher concentrations it was also decreased. Similarly, absorption of K and Ca was also decreased with increasing concentrations of Mg. Magnesium at lower concentrations stimulated P uptake but at higher concentraions it was decreased. In the present study maximum growth of rice plants was achieved at about 250μM Ca, 260 μM K, and 33μM Mg in the nutrient solution.
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  • 81
    ISSN: 1573-5036
    Keywords: Boron ; Calcium ; Copper ; Genotypic differences ; Iron ; Magnesium ; Manganese ; Mineral nutrition ; Molybdenum ; Nitrogen ; Phosphorus ; Plant adaptation ; Plant breeding ; Potassium ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant genotypes differ in their uptake, translocation, accumulation, and use of mineral elements. Examples of genotype differences to iron, nitrogen, phosphorus, potassium, calcium, magnesium, manganese, boron, copper, zinc, and molybdenum are discussed. Current knowledge is sufficient to indicate that many crop plants can be improved for the efficient use of mineral elements and better adaptation to mineral stress conditions.
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  • 82
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    Plant and soil 71 (1983), S. 371-380 
    ISSN: 1573-5036
    Keywords: Apple ; Calcium ; Copper ; Iron ; Irradiance ; Magnesium ; Manganese ; Nitrogen ; Nutrient uptake rate ; Phosphorus ; Potassium ; Scion effects ; Season ; Source of nitrogen ; Temperature ; Tissue nutrient level ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The rates of uptake of nutrients from solution by apple roots were measured (a) in a root laboratory, using intact roots of mature trees growing under field conditions and (b) in controlled environment using young trees. Maximum nitrate inflows into Discovery/M.9 roots under field conditions were only slightly lower than those into roots of the same genotype in controlled environment, but up to 80 times lower than those into roots of Worcester Pearmain seedlings. At any given external P concentration, P inflows into roots of field-grown trees were about 2.5-times lower than those into the roots of young trees in controlled environment. Nitrate inflows were constant above a solution concentration of 20 mmol m−3 in both field-grown and small trees. In both cases, phosphate inflows increased linearly with solution concentration up to 10 mmol m−3. Among the various plant and environmental factors influencing nutrient uptake characteristics of apple roots were: the scion genotype, tissue nutrient levels, root origin, the form in which N is supplied, level of irradiance of the shoot, root temperature and the season of the year. The effects of these factors are illustrated with examples.
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  • 83
    ISSN: 1573-5036
    Keywords: Calcicole ; Calcifuge ; Calcium ; Dittrichia viscosa ; Magnesium ; Potassium ; Sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Dittrichia viscosa is a Mediterranean bush widespread on various soil types. It is shown that the plants from a calcareous habitat (G plants) and those from an acidic habitat (M plants) differ in their ability to accumulate various cations when growing in the same experimental conditions. On this acidic soil the G plants accumulate more Ca and less K than the M plants. On a calcareous soil the response is reversed; it is the M plants which contain more Ca and often less K. This behaviour on each of the soils is typically that of a calcifuge for M and a calcicole for G. The two types of plants also differ in their affinity for Mg and Na. The ubiquity of the species could well be explained at least partially by genetic differentiation in the ability of plants to select ions.
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  • 84
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    Plant and soil 74 (1983), S. 437-450 
    ISSN: 1573-5036
    Keywords: Burning controlled ; Calcium ; Carbon ; CEC ; Eucalyptus ; Magnesium ; Nitrogen ; N. mineralisation ; Potassium ; Rainforest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In mixed eucalypt/rainforest in southern Tasmania, samples of surface soil 0 to 2 cm, 2 to 5 cm, and 5 to 10 cm were taken from a clear-felled coupe before and after burning in 1982, from a similar coupe after burning in 1979, and from an uncut area adjacent to each coupe. Factors compared were bulk density; total organic C, N, P, Ca, Mg, and K; pH; exchangeable Ca, Mg, and K; cation exchange capacity; extractable P; and N-mineralisation rates. The effect of burning was found to be restricted mainly to the upper 2 cm of soil. The combustion of organic matter caused losses of 7360 kg organic C and 211 kg N/ha; 348 kg Ca and 282 kg Mg and 151 kg K/ha were added to the soil in ash. Burning caused significant increases in pH, exchangeable Ca, Mg, and K, and in extractable P; cation exchange capacity was reduced. In the 6 months after burning only K was leached from the upper 2 cm of soil. Equilibrium levels of NH4−N increased initially after the fire, but between 6 and 18 months, equilibrium levels and rate of production of NH4−N during anaerobic incubation in soil of burned coupes differed little from that in adjacent uncut forest. Rates of production of NO3−N during aerobic incubation were very low throughout the period of study. It is concluded that for soils developed on dolerite in mixed eucalypt/rainforest, a single regeneration burn probably improves the nutritional status of the soil. Nutrients lost from the area as particulate ash are in quantities that will probably be replaced in rainfall in 15 to 20 years.
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  • 85
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    Plant and soil 70 (1983), S. 391-402 
    ISSN: 1573-5036
    Keywords: Intercropping Lupins ; Lupinus albus ; Manganese ; Nitrogen ; Phosphorus ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Significant interactions between wheat and lupins occur below ground and wheat intercropped with lupins has access to a larger pool of available P, Mn and N than has wheat grown in monoculture. This suggests that the wheat is able to take up nutrients produced or made available by lupins grown in association with it.
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  • 86
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    Plant and soil 71 (1983), S. 463-467 
    ISSN: 1573-5036
    Keywords: Anion uptake ; Barley ; Hordeum vulgare ; Mycorrhiza ; Phosphorus ; pH Rhizosphere ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In two field experiments sown in 1982 to test the effect of vesicular-arbuscular mycorrhizas (VAM) on growth and phosphorus nutrition of (i) spring wheat and spring barley, (ii) winter wheat and winter barley, we measured the concentrations of the major cation (K+, Ca2+, Mg2+ and Na+) and anions (Cl−, SO4 2−, H2PO4 − and NO3 −) in shoot tissue. In all cases the sum of the anion concentrations (ΣA) was increased strongly by mycorrhizal infection but not by P additions, confirming earlier observations2 on spring wheat. The concentration of total cations (ΣA) was generally reduced by P additions, hence P and VAM both reduced the cation excess (ΣC−ΣA) but by different mechanisms. These results suggest that increased uptake of anions by plants with VAM may be a general phenomenom which would have important implications for the elemental composition of crops. The effect may also be manifested by other types of mycorrhizal association.
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  • 87
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    Plant and soil 75 (1983), S. 51-61 
    ISSN: 1573-5036
    Keywords: Aggregate fractions ; Fatty acids ; Long-term rotation ; Triticum aestivum ; Water-stable aggregates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three non-replicated, unfertilized, dryland grain rotations—continuous wheat, wheat-fallow, and wheat-wheat-fallow—were established in 1912 on a Dark Brown Chernozemic (Typic Haploboroll) soil. The effect of long-term cropping on the chemical constituents of total water-stable aggregates was assessed. There was a loss in percentage of total water-stable aggregates and a shift in aggregate size distribution with time. Together with an increase in the 100 μm diameter fraction, there was an increase in the sand component of this fraction. These sand particles are probably held together by alkaline-soluble, acid-insoluble organic matter. Organic carbon, polysaccharides, polyuronides, phenols, and chloroform/methanol-extractable organic matter were all associated with the 〉250 μm diameter fractions. Although the aggregates had generally the same suite of aliphatic carboxylic acids, the relative proportions changed with cultivation.
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    Euphytica 32 (1983), S. 593-600 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; Secale cereale ; inbred lines ; intergeneric crossability ; embryo development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crossability and embryo development were studied in the crosses of one Triticum aestivum and three T. durum genotypes with nineteen rye inbred lines. Parental wheat and rye genotypes exerted a significant influence on the characters seed set, number of seeds containing embryos and viable plantlets obtained from embryo culture. It was established that the common winter wheat cultivar Götz is of intermediate crossability. The rye inbred lines varied substantially in their capacity to fertilize several wheat genotypes. Interactions between wheats of different crossability classes and their seed set with rye lines were detected. Significant correlations were obtained between seed set and viable plantlets recovered in vitro.
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  • 89
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; protein content ; grain yield ; mixing time ; soil-borne mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat (Triticum aestivum L.) cultivar Lancota has genetic potential to produce grain with higher protein content than most other cultivars grown in the hard winter wheat region. It has not consistently expressed full potential for grain protein content outside its area of development. Experiments were conducted to determine genetic variability for grain protein content in Lancota and to utilize that variability to select genotypes with high grain protein content. Approximately 1600 lines were screened to 37 high-protein selections that varied in yield, test weight, flour mixing time, blooming date, height, and reaction to wheat soil-borne mosaic virus (WSBM). Nine promising selections (KS80476, KS80478, KS80480, KS80488, KS80490, KS80491, KS80497, KS80499, and KS80500) had grain protein advantage over Lancota of 0.5 to 1.0% and equalled or exceeded Lancota in yield or test weight. Those selections were resistant to WSBM and satisfactory or better in mixing properties than Lancota. The highest protein selection (KS80496) had a mean protein advantage of 1.5% over Lancota but exhibited a short mixing time of 1 7/8 min. The absence of correlation between some years indicated strong environmental influence on protein content. We concluded that adequate genetic variability existed in the high-grain protein cultivar Lancota to select lines that express the high protein potential better than the original cultivar outside its area of development.
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  • 90
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; linkage drag ; seedling resistance ; Puccinia graminis tritici ; stem rust ; Puccinia recondita ; leaf rust ; Puccinia striiformis ; yellow rust ; stripe rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To determine whether linkage drag had occurred during the breeding of near isogenic lines (NILs) of wheat, 176 lines involving 11 sets of NILs, their recurrent parents and some of their donors were tested for seedling reaction to stem rust (4 races), leaf rust (3 races) and yellow rust (3 races). From the results, six cases were identified in which linkage drag may have played a role. More research is needed to prove clearly that linkage is involved. Nevertheless, the results suggest that linkage drag is a fairly common phenomenon.
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  • 91
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat, glutenin ; high-molecular-weight subunits ; SDS-PAGE ; SDS-sedimentation test ; baking quality selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The high-molecular-weight subunit composition of glutenin is regulated by genes on the long arm of the homoeologous group 1 chromosomes. Evidence is presented that in general the bread-making quality of wheat cultivars containing the subunits 3+10 coded for by chromosome 1D or the subunit 2* coded for by chromosome 1A is higher than that of cultivars containing their allelic counterparts the subunits 2+11 or subunit 1 and the null form respectively. Besides it is shown that the positive effects of the subunits 3+10 and subunit 2* are additive.
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  • 92
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum turgidum ; Hordeum vulgare ; barley ; chemotypes ; electrophoresis ; variation ; prolamines ; gliadins ; hordeins ; electrophoregram ; genetic resources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Polyacrylamide gel electrophoresis (PAGE) of storage proteins (prolamines) was used to screen 64 landraces of wheat and barley from Nepal and the YemenArab Republic and two cultivars for comparison. Altogether 3168 single seeds were examined and the advantages gained by using the vertical slab gel method were recognised. The extent of variation present within populations of landraces could be assessed easily and rapidly using the methods described. Differences in ploidy levels of wheats were detected by PAGE and investigated. Suggestions are made for improvements in sampling strategies in hilly terrain.
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  • 93
    ISSN: 1573-1561
    Keywords: allelopathy ; ferulic acid ; no-tillage ; prickly sida ; common ragweed ; weed control ; 2-methoxy-4-ethenylphenol ; carboxylic acid ; phenol ; morning glory ; Ipomoea lacunosa ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This study was conducted to determine if well-known phytotoxic effects of plant residues on crop growth could also be responsible for observed reductions of certain weed species in no-till cropping systems. An aqueous extract of field-grown wheat (Triticum aestivum L.) reduced the germination and root length of pitted morning glory (Ipomoea lacunosa L.) and common ragweed (Ambrosia artemisiifolia L.). Phytotoxicity was increased by about 70% when bioassays with the wheat extract on morning glory and ragweed were conducted in the presence of light. Phytotoxic substances were extracted from wheat with 2 N NaOH. The hydrolyzed extract was fractionated by thin-layer chromatography (TLC). The compound isolated by TLC having the greatest inhibitory effects on morning glory germination was identified using mass spectrometry and determined to be ferulic acid (4-hydroxy-3-methoxycinnamic acid). Ferulic acid at 5 × 103 M inhibited the germination and root length of morning glory 23 and 82%, respectively, and prickly sida (Sida spinosa L.) with carpels 85 and 82%, respectively. Crabgrass (Digitaria sanguinalis L.) germination was inhibited 100%. Ferulic acid had no effect on ragweed or prickly sida without carpels. Morning glory root and shoot biomass were reduced 52 and 26%, respectively, when morning glory was grown in sand and watered with a 5 × 103 M solution of ferulic acid. Ferulic acid in the presence of prickly sida seed carpels was found to undergo decarboxylation, forming a styrene derivative, 2-methoxy-4-ethenylphenol. The more phytotoxic styrene compound was produced by a bacterium isolated from the carpels of prickly sida seed. The study showed that ferulic acid and other compounds may indeed play a role in reducing the growth of certain weeds in no-tillage cropping systems.
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  • 94
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    Plant and soil 74 (1983), S. 101-109 
    ISSN: 1573-5036
    Keywords: Crop residue ; Root growth ; Shoot growth ; Soil types ; Temperature ; Triticum aestivum ; Wheat germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two controlled environment experiments were conducted to examine the germination and early growth of wheat (Triticum aestivum L. cv. Songlen) growing under crop residues of rape, sorghum, field pea and wheat. Additional treamments also included were soil type (Lithic Vertic Ustochrept and Plinthustalf) and temperature (8°C and 24°C to simulate winter and autumn sowing conditions). At low temperature, wheat and sorghum residues produced the most adverse effects on germination with all residues reducing emergence at high temperatures. Shoot lengths were also reduced by most residues at high temperatures whilst root lengths and shoot and root dry weights were unaffected by residue treatments. These results suggest major phytotoxic effects of residues during early growth (up to 14 days after sowing) with, in general, few interactions with soil type or temperature.
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  • 95
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    Plant and soil 74 (1983), S. 229-235 
    ISSN: 1573-5036
    Keywords: Barley ; Hordeum vulgare ; Mineral composition ; Na−K interaction ; Solonetzic soil ; Solonetz-Solod sequence ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and mineral composition of barley and wheat was measured across sequences of Solonetz and Solod soils. Growth of both species, along with root penetration was reduced on the Solonetz compared to the Solod soil. Mineral composition of the foliage and roots indicated that a Na−K interaction was present for the barley across the Solonetz-Solod sequences. Such factors were considered to be characteristics of soil—plant relationships on Solonetzic soils.
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  • 96
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; chromosome substitution lines ; vernalization ; ear emergence ; basic development rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of vernalization on days to ear emergence in hexaploid wheat (Triticum aestivum L. em. Thell.) was examined in five Chinese Spring/Thatcher (CS/T) chromosome substitution lines. CS/T 5A and 5D were similar to normal Chinese Spring in days to ear emergence after all periods of vernalization while CS/T 3B and 5B were similar after some, but significantly earlier after other periods of vernalization When compared with Chinese Spring. In both the unvernalized condition and when vernalization did not limit development rate CS/T 7B was faster to ear emergence than Chinese Spring. These results are discussed in relation to the known chromosomal and genetical control of vernalization response.
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  • 97
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    Euphytica 32 (1983), S. 225-230 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; shattering ; awns ; height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Temporal changes in shattering losses of 14 wheat (Triticum aestivum L.) genotypes were studied in an irrigated environment. The lines ranged in height from 68 to 98 cm; eight lines were fully awned while five were not. Shattered kernels were gathered at weekly intervals for 6 wk; samping commenced at 30 to 40% kernel moisture and continued for 3 wk beyond harvest ripeness (14.5% moisture). Shattering loss, expressed as a percentage of yield, ranged from 3.25 to 17.3% over the 6 wk period. Awnedness was not a factor in shattering susceptibility of the genotypes studied. Both the most and least shattering resistant lines in this study were awnless. There was a nonsignificant correlation between shattering losses and plant height. It was concluded that a single quantitative measurement of shattering loss at or after harvest ripeness (14.5% moisture) would provide an adequate measure of shattering susceptibility.
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  • 98
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    Euphytica 32 (1983), S. 241-255 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; physiology ; grain yield ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Post anthesis physiological characters were examined in four genotypes and a diallel set of their progenies. Variation in total carbon uptake by photosynthetic tissue above the flag leaf node was primarily related to leaf area and ear size differences during the early grain filling period. Flag leaf apparent photosynthetic rates during the late grain filling period were closely correlated with flag leaf chorophyl levels. During the period of rapid growth, genotypes differed in the proportion and total quantity of current assimilate translocated to the grain. Total rather than proportional translocation was correlated with grain number and grain yield. Differences in total grain protein were primarily related to total plant nitrogen at anthesis and secondarily to the proportion of this nitrogen translocated to the grain. The level of post anthesis nitrate reductase activity decreased with increasing flag leaf age, but genotypic differences were not closely related to differences in total grain protein contents. Significant general combining ability effects were found for flag leaf chlorophyll levels, flag leaf photosynthetic rates at higher illuminances during the late grain filling period, total plant carbon uptake, total ear carbon uptake, and proportional carbon translocation to the grain. A preponderance of significant general combining ability variances suggests that additive gene action is of particular importance in the inheritance of these physiological characters.
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  • 99
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    Euphytica 32 (1983), S. 257-271 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; Triticum compactum ; collection ; germplasm ; land-races ; local varieties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat has traditionally been grown by the Beduin population in the semi-arid (150 to 200 mm, mean total annual rainfall) northern Negev region of Israel. A collection was made in this area (the size of which is 150 km2) from small (0.1 to 0.5ha) fields of mixed wheat, resulting in 1553 collected spikes. Each spike was planted in a 1 m row at Bet Dagan, and grown under favorable conditions. Qualitative and quantitative data were collected from each row. Qualitative data were submitted to hierarchial clustering and the results were compared with published information on the identification, classification and distribution of the land-races of wheat in the Middle East. Triticum durum was represented in 84% of the collection. It was clustered into 22 populations, identified as 11 known varietas of T. durum. They were aggregated into five groups, similar to groups of old varieties recognized by Jacubziner (1932). While 38.5% of the collection consisted of T. durum groups villosa and sinaica, aboriginal to the northern Negev, it included also forms similar to several land-races found in the past in other parts of the Middle East. Each of the populations, and the durum collection as a whole, was very diverse for the quantitatively measured plant attributes. Triticum aestivum was represented in 15.6% of the collection, clustered into six populations. Most of the common wheat accessions were analogous to the old locally grown variety Hirbawi. Triticum compactum was represented in only eight accessions. The collection is now being evaluated as a potential genetic resource for durum wheat breeding.
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  • 100
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; grains ; dry matter and nitrogen ; distribution within ear ; plant height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Distribution and accumulation of dry matter (d.m.) and nitrogen (N) were investigated within ears of a semidwarf, a gigas, and a normal wheat genotype grown under controlled environments. A high harvest index was linked with a small pre-anthesis N-storage (and vice versa) but this did not affect N amounts in grains because of compensation by prolonged N uptake during grain filling. N distribution within and between spikelets roughly paralleled that of d.m. Nevertheless, the two processes appeared to be rather independent as became evident from comparisons of the time courses in and between the genotypes. Final distribution of d.m. and N within and between spikelets mainly depended on different rates during the linear phases of accumulation and less on the duration. Maximum of d.m. and N weights per grain as well as maximum of grain number were observed in spikelets below the middle of the ear axis. This ‘submedian dominance’ suggested a pre-anthesis determination of the accumulation potential of grains in different spikelets. N percentage within spikelets disclosed that N accumulation was hampered more than that of d.m. in those positions unfavourable to the latter process. This principal pattern was not affected by genotypes in spite of considerable differences in numbers and weights of grains.
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