Skip to main content
SpringerLink
Log in

Calcium distribution in freeze-dried enamel organ tissue during normal and altered enamel mineralization

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

Energy dispersive X-ray microanalysis was applied to freeze-dried blocks of enamel organ tissue to determine levels of calcium in various celular regions. The tissue blocks were dissected free from adjacent forming enamel following injection of cobalt or fluoride ions, both of which temporarily inhibit enamel mineralization. In all control and experimental specimens there was an increasing gradient of calcium from the stratum intermedium cells to the distal ends of the ameloblasts. Calcium levels were significantly reduced near the distal ends of the ameloblasts following cobalt or fluoride injection as compared with controls. It is suggested that evidence of an intercellular buildup of calcium near the distal ends of the ameloblast supports a controlling function of these cells. The changes in calcium levels are correlated with alterations in mineralization known to occur in the adjacent enamel of the model systems employed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Reith EJ, Cotty VE (1962) Autoradiographic studies on calcification of enamel. Arch Oral Biol. 7:365–372

    Article  CAS  PubMed  Google Scholar 

  2. Wennberg A, Bawden JW (1978) Influence of the pulpal route on uptake of45Ca in enamel and dentin of developing rat molars. J Dent Res 57:313–318

    CAS  PubMed  Google Scholar 

  3. Nagai N, Frank RM (1975) Transfert du Ca45 par Autoradiographie en Microscopie Electronique au Cours de L'Amelogenese. Calcif Tissue Res 19:211–221

    CAS  PubMed  Google Scholar 

  4. Eisenmann DR, Ashrafi S, Neiman A (1979) Calcium transport and the secretory ameloblast. Anat Rec 193:403–422

    Article  CAS  PubMed  Google Scholar 

  5. Boyde A, Reith EJ (1977) Qualitative electron probe analysis of secretory ameloblasts and odontoblasts in the rat incisor. Histochem 50:347–354

    Article  CAS  Google Scholar 

  6. Reith EJ, Boyde A (1978) Histochemical and electron probe analysis of secretory ameloblasts of developing rat molar teeth. Histochemistry 55:17–26

    Article  CAS  PubMed  Google Scholar 

  7. Engel M (1981) Microprobe analysis of calcifying matrices and formative cells in developing mouse molars. Histochemistry 72:443–452

    Article  CAS  PubMed  Google Scholar 

  8. Neiman A, Eisenmann DR (1975) The effect of strontium, cobalt and fluoride on rat incisor enamel formation. Anat Rec 183:303–322

    Article  CAS  PubMed  Google Scholar 

  9. Eisenmann DR, Ashrafi SH, Zaki AE (1982) Multi-method analysis of calcium localization in the secretory ameloblast. J Dent Res 61:1555–1561

    CAS  Google Scholar 

  10. Walton RE, Eisenmann DR (1974) Ultrastructural examination of various stages of amelogenesis following parenteral fluoride administration. Arch Oral Biol 19:171–182

    Article  CAS  PubMed  Google Scholar 

  11. Hall TA (1971) Microprobe assay of chemical elements. In: Oster G (ed) Physical techniques in biological research optical techniques, Vol. 1A, 2nd ed., Academic Press, New York, pp 157–275

    Google Scholar 

  12. Russ JC (1976a) Processing of energy dispersive x-ray spectra. EDAX Editor 6:4–39

    Google Scholar 

  13. Russ JC (1976b) An improved model to calculate pure element intensities in bulk samples. EDAX Editor 6:42–45

    Google Scholar 

  14. Boyde A, Reith EJ (1976) Scanning electron microscopy of the lateral cell surfaces of rat incisor ameloblasts. J Anat 122:603–610

    CAS  PubMed  Google Scholar 

  15. Skobe Z (1980) Scanning electron microscopy of the mouse incisor enamel organ in transition between secretory and maturation stages of amelogenesis. Arch Oral Biol 25:395–401

    Article  CAS  PubMed  Google Scholar 

  16. Bawden JW, Wennberg A (1977) In vitro study of cellular influence on Ca45 uptake in developing rat enamel. J Dent Res 56:313–319

    CAS  PubMed  Google Scholar 

  17. Kallenbach E (1980) Fate of horseradish peroxidase in the secretion zone of the rat incisor enamel organ. Tissue Cell 12:491–501

    Article  CAS  PubMed  Google Scholar 

  18. Sasaki T, Higashi S, Tachikawa T, Yoshiki S (1982) Formation of tight junctions in differentiating and secretory ameloblasts of rat molar tooth germs. Arch Oral Biol 27:1059–1068

    Article  CAS  PubMed  Google Scholar 

  19. Mulryan BJ, Neuman MW, Neuman WF, Toribara TY (1964) Equilibration between tissue calcium and injected radiocalcium in the rat. Am J Physiol 207:947–952

    CAS  PubMed  Google Scholar 

  20. Borle AB (1967) Membrane transfer of calcium. Clin Orthop Rel Res 52:267–291

    CAS  Google Scholar 

  21. Crenshaw MA, Takano Y (1982) Mechanisms by which the enamel organ controls calcium entry into developing enamel. J Dent Res 61:1574–1579

    CAS  Google Scholar 

  22. Reith EJ (1983) A model for transcellular transport of calcium based on membrane fluidity and movement of calcium carriers within the more fluid microdomains of the plasma membrane. Calcif Tissue Int 35:129–134

    CAS  PubMed  Google Scholar 

  23. Irving J (1973) Theories of mineralization of bone. Clin Orthop Rel Res 97:225–236

    Google Scholar 

  24. Gelpke C, Eisenmann DR (1980) Effects of cobalt on calcium transport in the secretory ameloblast as visualized by PPA. J Dent Res 59(A):811

    Google Scholar 

  25. Nikiforuk G, Fraser D (1979) Etiology of enamel hypoplasia and interglobular dentin: the roles of hypocalcemia and hypophosphatemia. Metab Bone Dis Rel Res 2:17–23

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Histology, University of Illinois, Box 6998, 60680, Chicago, Illinois, USA

    D. R. Eisenmann, S. H. Ashrafi & A. E. Zaki

Authors
  1. D. R. Eisenmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. H. Ashrafi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. E. Zaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Eisenmann, D.R., Ashrafi, S.H. & Zaki, A.E. Calcium distribution in freeze-dried enamel organ tissue during normal and altered enamel mineralization. Calcif Tissue Int 36, 596–603 (1984). https://doi.org/10.1007/BF02405373

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02405373

Key words

Search

Navigation