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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of peanut with a soybean vspB promoter-uidA chimeric gene. I. Optimization of a transformation system and analysis of GUS expression in primary transgenic tissues and plants (1998)
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 102 (1998), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Direct DNA delivery via microprojectile bombardment has become an established approach for gene transfer into peanut (Arachis hypogaea L.). To optimize our transformation protocol and to simultaneously explore the function of a heterologous promoter whose activity is developmentally regulated, embryogenic cultures from three peanut cultivars were bombarded with two plasmid constructs containing a uidA gene controlled by either a soybean vegetative storage protein gene promoter or a cauliflower mosaic virus 35S promoter. We found that GUS transient expression was useful to predict stable transformation and confirmed that image analysis could provide a quick and efficient method for semi-quantitation of transient expression. One hundred and sixty hygromycin-resistant cell lines were recovered from and maintained on selective medium, and those tested by Southern blot analysis showed integration of the foreign gene. Over 200 transgenic plants were regenerated from 38 cell lines. More than 100 plants from 32 cell lines flowered and 79 plants from 19 cell lines produced pods. Over 1000 R1 seeds were harvested. Analysis of expression in primary transgenic plants showed that GUS expression driven by the vspB promoter was modulated by chemical and positional information.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.1998.1020106.x
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro regeneration and genetic manipulation of grasses (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 73 (1988), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Regeneration of plants from cultured cells is an important and essential component of plant biotechnology. Advances in the recovery of plants from cultured cells and protoplasts of grasses, and in genetic transformation provide challenging opportunities for the genetic manipulation and improvement of this most important group of food plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1988.tb05442.x
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  • 3
    Electronic Resource
    Electronic Resource
    Preferential amplification of mitochondrial DNA fragments in somatic hybrids of the Gramineae (1988)
    Springer
    Current genetics 13 (1988), S. 241-245 
    Details
    ISSN: 1432-0983
    Keywords: DNA amplification ; Mitochondrial DNA ; Somatic hybrid ; Gramineae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Somatic hybrid cell lines of Pennisetum americanum + Panicum maximum, and of Pennisetum americanum + Saccharum officinarum display unique mitochondrial DNA (mtDNA) restriction patterns suggestive of mitochondrial fusion and recombination. Apparent recombinant fragments of the hybrids were recovered, cloned, and hybridized to parental and somatic hybrid mtDNAs. In each somatic hybrid, “novel” fragments were found to be present at low copy number in one or both of the parental mtDNAs, and amplified 15–30 times in the hybrids. In pearl millet-sugarcane somatic hybrid cells, the amplification does not appear to involve enhanced recombination. The presumably amplified restriction fragment of the pearl millet-Guinea grass somatic hybrids is a junction fragment of a repeat, present in low copy number in both parents, and in high copy number in the hybrids. Thus protoplast and probable mitochondrial fusion results in a marked shift in the direction of mtDNA recombination events. We conclude that amplification of parental mtDNA fragments is a common event in somatic hybrid cells of these Gramineae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387770
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  • 4
    Electronic Resource
    Electronic Resource
    Plant regeneration from immature embryos of peanut (1989)
    Springer
    Plant cell reports 8 (1989), S. 217-218 
    Details
    ISSN: 1432-203X
    Keywords: Groundnut ; Somatic Embryo ; Arachis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5–1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal medium. Shoots were excised from thick embryonic roots and rerooted on Murashige and Skoog medium containing half the normal concentration of inorganic salts. This technique should be useful for the production of interspecific hybrid plants from immatureArachis embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00778536
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  • 5
    Electronic Resource
    Electronic Resource
    Rapid estimation of ploidy levels in in vitro-regenerated interspecific Arachis hybrids and fertile triploids (1992)
    Springer
    Euphytica 64 (1992), S. 183-188 
    Details
    ISSN: 1573-5060
    Keywords: Arachis ; chloroplast number ; interspecific hybrids ; pollen grain size ; 2n gametes ; groundnut ; peanut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A significant correlation among chromosome number, chloroplast number and pollen grain size was observed using interspecific hybrids (2n=30,60), Arachis hypogaea (2n=40), A. stenosperma (PI 338280) (2n=20), A. batizocoi (PI 468329) (2n=20) and A. villosulicarpa Hoehne (PI 336984) (2n=20), representing four ploidy groups. Both chloroplast number in guard cells and pollen grain size were found to be positively correlated with ploidy, and provided a reliable method to distinguish diploid, triploid, tetraploid and hexaploid plants from each other regardless of their taxonomic backgrounds. These methods in combination with root-tip chromosome counts enabled us to confirm ploidy determinations on all three dissue layers, L1, L2 and L3 (guard cells, microsporocytes and root meristematic cells, respectively), and to detect the chimeric nature of some colchicine-treated tissue culture-derived plants. Screening pollen grains by size and the detection of highly stainable and viable, 30-chromosome pollen grains have enhanced the use of triploid hybrids in peanut breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00046047
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  • 6
    Electronic Resource
    Electronic Resource
    Improved efficiency and normalization of somatic embryogenesis inTriticum aestivum (wheat) (1983)
    Springer
    Protoplasma 117 (1983), S. 40-44 
    Details
    ISSN: 1615-6102
    Keywords: Cereals ; Immature embryo ; Somatic embryogenesis ; Tissue culture ; Triticum aestivum L. ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tissue cultures derived from the scutellum of immature embryos ofTriticum aestivum L. (wheat) gave rise to somatic embryos with a well-defined scutellum and coleoptile as well as one or more shoot primordia and a root primordium. The normal somatic embryos were formed from compact, white callus tissue which was not observed until 4 or more weeks after culture initiation. The highest frequency of white embryogenic tissue formation and the most normal embryoids were obtained in the dark on Murashige and Skoog's medium with twice the concentration of inorganic salts plus sucrose (2%), inositol (100–200 mg/l), casein hydrolysate (100–200 mg/l), glutamine (500 mg/l), and 2,4-dichlorophenoxyacetic acid (1–2 mg/l).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281782
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  • 7
    Electronic Resource
    Electronic Resource
    Plant regeneration from cultured immature embryos and inflorescences ofTriticum aestivum L. (wheat): Evidence for somatic embryogenesis (1982)
    Springer
    Protoplasma 110 (1982), S. 95-105 
    Details
    ISSN: 1615-6102
    Keywords: Cereals ; Immature embryo ; Inflorescence ; Plant regeneration ; Somatic embryogenesis ; Triticwn aestivum L. ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tissue cultures ofTriticum aestivum L. (wheat) initiated from young inflorescences and immature embryos possessed the potential for regeneration of whole plants. Both a friable and a compact type of callus were produced on Murashige and Skoog's medium with 2 mg/l 2,4-dichlorophenoxyacetic acid. The friable callus contained meristematic centers in which the peripheral cells ceased dividing, elongated, and could be easily separated. Roots were frequently formed in this type of callus. The compact, yellowish, and nodular callus arose from the epithelial and sub-epithelial cells of the embryo scutellum, and the rachis and glumes of the young inflorescence. Such callus had a smooth surface and characteristic chlorophyllous areas. Plants were regenerated only from the compact callus. The first sign of differentiation in the compact callus was the formation of a cleft or notch on the smooth surface, followed by the appearance of trichomes and the direct development of leafy structures which were not associated initially with any shoot meristems. Multiple shoots subsequently arose at the bases of the leafy structures, which are considered modifications of the scutellum, a definitive part of the cereal embryo. Accordingly, we suggest that while typical bipolar embryos are generally not formed, plant regeneration nevertheless takes place through embryogenesis and the precocious germination of the embryoids. Plants regenerated from immature embryo and inflorescence cultures were grown to maturity in soil, and were shown to have the normal chromosome number of 2n=6x=42.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281535
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  • 8
    Electronic Resource
    Electronic Resource
    Callus induction and growth from the mature embryo ofTriticum aestivum (Wheat) (1983)
    Springer
    Protoplasma 115 (1983), S. 104-113 
    Details
    ISSN: 1615-6102
    Keywords: Cereals ; Embryo culture ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell proliferation from the mature embryo of wheat occurs on a defined medium in the presence of 2,4-D. Unorganized growth is observed when the 2,4-D concentration is equal to or greater than 2 mg/l, but increasing levels of 2,4-D inhibit cell division. Cell divisions begin after 4 days in culture from parenchyma cells within and near the procambial tissues of the embryo axis. By day 8 continuous meristematic zones are formed in association with vascular tissues, and DNA synthesis and cell divisions are distributed throughout these zones. No morphological evidence exists to show that these zones consist of proliferating root primordia, which are formed only after the level of 2,4-D falls below some critical concentration. When the concentration of 2,4-D is lowered, the meristematic zones first become dissected and then give rise to many root meristems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279802
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  • 9
    Electronic Resource
    Electronic Resource
    Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album L.) (1985)
    Springer
    Protoplasma 124 (1985), S. 80-86 
    Details
    ISSN: 1615-6102
    Keywords: Santalum album L. ; Sandalwood ; Somatic embryogenesis ; tree protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts were isolated from embryogenic cell suspension cultures derived from proliferating shoot segments of a 20-year-old sandalwood tree (Santalum album Linn.). Under appropriate conditions, isolated protoplasts divided in liquid culture medium and produced embryogenic cell aggregates and globular embryos. Plating of cell aggregates on a fresh medium facilitated the differentiation of somatic emryos which further developed into plantlets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279726
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  • 10
    Electronic Resource
    Electronic Resource
    Somatic hybridization in the gramineae: Pennisetum americanum (L.) K. Schum. (Pearl millet) +Panicum maximum Jacq. (Guinea grass) (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 365-370 
    Details
    ISSN: 1617-4623
    Keywords: Cereals/grasses ; Gramineae ; Somatic hybridization ; Panicum maximum ; Pennisetum americanum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts from Pennisetum americanum resistant to S-2-amino-ethyl-l-cysteine (AEC) were fused with protoplasts of Panicum maximum utilizing polyethylene glycol-dimethylsulfoxide after inactivation of the Pennisetum protoplasts with 1 mM iodoacetic acid. The iodoacetate treatment prevented division of Pennisetum protoplasts; therefore, only Panicum protoplasts and heterokaryons potentially could give rise to colonies. A second level of selection was imposed by plating 3–4-week-old colonies on AEC medium. Putative somatic hybrid calli were analyzed for alcohol dehydrogenase, 6-phosphogluconate dehydrogenase, aminopeptidase, and shikimate dehydrogenase isozymes. Three somatic hybrid cell lines (lines 2, 3, and 67) were identified which showed two bands of alcohol dehydrogenase activity representing homodimers of P. maximum and P. americanum as well as a novel intermediate band of activity where Panicum-Pennisetum heterodimers would be expected. Aminopeptidase and shikimate dehydrogenase were useful for identifying presumptive hybrid calli but the isozyme patterns were additive-evidence which would not preclude the selection of chimeric callus. A more complex isozyme pattern which varied among the somatic hybrids was observed for 6-phosphogluconate dehydrogenase. In the hybrid calli, the presence of DNA sequences homologous to both P. maximum and P. americanum sequences was confirmed by hybridization of a maize ribosomal DNA probe to XbaI and EcoRI restriction fragments. Growth of hybrid lines on various concentrations of AEC was either similar to the AEC-resistant parent (hybrid line 2) or intermediate between the resistant and sensitive parents (hybrid lines 3, 67).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422058
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