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  • Hymenoptera  (201)
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  • Springer  (374)
  • 1990-1994  (374)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 73 (1994), S. 289-297 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; parasitoid ; C. rubecula ; foraging behaviour ; specialist ; synomones ; infochemical use ; tritrophic interactions ; olfaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of volatile stimuli in the long-range host-searching behaviour of the specialist parasitoidCotesia rubecula Marshall (Hymenoptera: Braconidae) was studied. Components from the plant-host-complex Brussels sprouts (Brassica oleracea L. var.gemmifera (DC.) Schulz. cv. ‘Titurel’)-Pieris rapae L. (Lepidoptera: Pieridae) were compared for their attractiveness in dual choice tests in a windtunnel. Stimuli from cabbage plants that were mechanically damaged or damaged byP. rapae caterpillars were more attractive to this parasitoid species than stimuli emitted by the host larvae or their faeces. Parasitoids preferred leaves from the plant-host-complex over artificially damaged leaves. Undamaged cabbage plants were the least attractive to the foraging females. These results indicate that in-flight searching behaviour ofC. rubecula is guided by plant-derived information and that for this specialist species more reliable and specific host-derived cues play a minor role at longer distances.
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  • 2
    ISSN: 1570-7458
    Keywords: ecdysteroid agonists ; juvenile hormone analogue ; Spodoptera exigua ; growth ; moulting ; metamorphosis ; imaginal discs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Biological activity assays with RH 5849 and RH 5992 indicated that both compounds affected growth and development of last-instar larvae ofSpodoptera exigua (Hübner) (Lepidoptera: Noctuidae) in a dose-dependent manner. Within the first 24 h after treatment by continuously offering leaves dipped in a water solution of ≥50 mg/l RH 5849 and ≥0.5 mg/l RH 5992, symptoms of a prematurely induced larval moult and head capsule apolysis were visible. Intoxicated larvae died shortly afterwards, showing signs of unsuccessful ecdysis. LC50-values of RH 5849 and RH 5992 for fifth-instarS. exigua larvae were 110 and 2.5 mg/l, respectively. Pyriproxyfen alone affected the larval stage and disturbed normal metamorphosis. One supernumerary larval instar occurred occasionally. LC50-value for pyriproxyfen was 1.7 mg/l. Larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, continued to grow until they attained a size and weight about 2–3 times that of the controls. This growth was accompanied by at least one and sometimes two supernumerary moults. Concerning thein vivo imaginal wing disc growth and development, only in larvae treated with 10 and 50 mg/l RH 5849 or 0.5 mg/l RH 5992, tracheole migration was observed earlier than in the controls. When applying 300 mg/l RH 5849 or 3–7 mg/l RH 5992, the discs remained small and no signs of tracheole migration were observed. In larvae simultaneously treated with RH 5849 or RH 5992 and pyriproxyfen, tracheole migration was not prematurely induced and a pupal cuticle was produced in the discs of larvae, undergoing a supernumerary moult. No clear signs of evagination were observed.
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  • 3
    ISSN: 1570-7458
    Keywords: Cruciferae ; Brassica oleracea ; tritrophic interactions ; Pieridae ; Hymenoptera ; parasitoids ; Cotesia ; kairomones ; headspace ; GC-MS ; chemical composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Headspace composition, collected from intact cabbage plants and cabbage plants infested with eitherPieris brassicae L. orP. rapae L. (Lepidoptera: Pieridae) first instar larvae, was determined by GC-MS. Twenty-one volatiles were identified in the headspace of intact plants. Twenty-two volatiles were identified in the headspace of plants infested byP. brassicae larvae, 2 of which, Z-3-hexenyl butyrate and Z-3-hexenyl isovalerate, were not detected in the headspace of either intact orP. rapae damaged plants. In the headspace of the latter, 21 compounds were identified, all of which which were also produced by intact plants. No significant quantitative differences were found between headspace composition of the plants damaged by one or the other caterpillar species. Major differences between intact and caterpillar-damaged plants in contribution to the headspace profile were revealed for hexyl acetate, Z-3-hexenyl acetate, myrcene, sabinene and 1,8-cineole. The larval endoparasitoidCotesia glomerata L. was attracted by the volatiles emanating fromB. oleracea damaged byP. brassicae first instar larvae.C. rubecula L., a specialized larval endoparasitoids ofP. rapae, was attracted by the volatiles released from theB. oleracea-P. rapae plant-host complex. This shows that cabbage plants kept under the conditions of headspace collection produce attractive volatiles for both parasitoids.
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  • 4
    Electronic Resource
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    Entomologia experimentalis et applicata 73 (1994), S. 211-220 
    ISSN: 1570-7458
    Keywords: Eurytoma amygdali ; Hymenoptera ; Eurytomidae ; host selection ; chemical stimuli ; olfactory response ; aggregation ; oviposition ; almond
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Females of the almond seed wasp,Eurytoma amygdali Enderlein (Hymenoptera, Eurytomidae), responded in an olfactometer positively to odours from almond flowers and unripe fruits, but not to almond leaf odours and odours from flowers and unripe fruits of certain other nonhostPrunus species. Males responded to none of these odours. Extracts of undamaged unripe almond fruits (using ethanol, methanol, acetone, hexane, dichloromethane, or petroleum ether) stimulated female aggregation on glass surfaces treated with these extracts; in addition, certain fruit extracts (ethanol, methanol, or acetone) stimulated oviposition. Extracts of undamaged almond leaves (ethanol, methanol, or acetone) and flowers (ethanol or methanol) also stimulated female aggregation and oviposition. Aggregation and oviposition in response to an ethanol extract of almond fruits was intense in females aged 5 to 14 days and from 12∶00 to 18∶00h (photophase between 06∶00 and 20∶00). Certain almond fruit (ethanol, methanol, acetone or hexane) and flower extracts (ethanol or methanol) also provoked female response in the olfactometer. The results strongly suggest that certain chemical stimuli emanating from parts of the almond tree play a major role in host selection and oviposition. Some of the extracts tested may be a good source for the isolation, identification and synthesis of compounds stimulating attraction, aggregation and oviposition in nature. *** DIRECT SUPPORT *** A3414024 00003
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  • 5
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    Entomologia experimentalis et applicata 70 (1994), S. 27-39 
    ISSN: 1570-7458
    Keywords: almond seed wasp ; Eurytoma amygdali ; diapause termination ; insect dormancy ; temperature ; prolonged diapause ; photoperiod ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diapausing larvae ofEurytoma amygdali Enderlein (Hymenoptera, Eurytomidae) were collected in early August and late September. They were subjected to various photoperiod and temperature regimens for up to 20 weeks, then kept at L16:D8 and 19°C for another 14 to 26 weeks for diapause to be terminated and pupation to take place. Photoperiod did not affect diapause completion. It was confirmed that the two morphologically distinct diapause stages have different temperature requirements for their completion. The first diapause stage was completed synchronously at temperatures between 16 and 19°C. A higher temperature of 26°C delayed diapause development. The second stage required lower temperatures between 4 and 10°C. Spontaneous termination of dipause was observed at constant 19°C. When applied to the first diapause stage for 20 weeks, low temperatures made the larvae refractory to subsequent intermediate temperatures. The first stage was thus maintained until a higher temperature of 26°C made the larvae regain their ability to respond to the intermediate temperatures and complete this stage. Larvae grown in Retsou almonds had a higher diapause intensity than larvae grown in Truoito almonds. The results suggest that, in nature, the high temperatures of late summer and early autumn are likely to maintain the first diapause stage. Subsequently, the less warm temperatures of autumn allow the completion of the first stage by late autumn, and the low temperatures of late autumn and of winter allow the completion of the second diapause stage by mid winter.
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  • 6
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    Entomologia experimentalis et applicata 70 (1994), S. 83-90 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Symphyta ; Tenthredinidae ; Dineura virididorsata ; sawfly ; sex ratio ; oviposition behaviour ; mating behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sex ratios of the arrhenotokous sawflyDineura virididorsata Retz. (Hymenoptera: Tenthredinidae) are variable among populations in the field, but are biased in favour of females. We have concluded elsewhere that the sex ratios of this species cannot be satisfactorily explained by any general sex ratio model that is available. Here we present data on the different behavioural traits that are relevant to interpretation of the evolution of female-biased sex ratios under local mate competition. We report on oviposition behaviour, mating behaviour and the patterns of movement of ovipositing virgin and mated females. Our results show that adult emergence is synchronous and females will mate daily with different males (in the laboratory, at least). This would decrease the relatedness of offspring should it also take place in the field. In addition, ovipositing females (mated and virgin) leave their site of release in a relatively short time and deposit few eggs relative to their capabilities, so siblings are unlikely to be clumped. The time that virgins spend in deposition of an egg is not different from that spent by mated females, so there is no differential ‘investment’ in this regard. These behavioural observations agree with our previous conclusion that the preconditions of current sex ratio theories are not met byD. virididorsata.
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  • 7
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    Entomologia experimentalis et applicata 70 (1994), S. 121-128 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Trichogrammatidae ; Trichogramma fuentesi ; couleur de l'hôte ; mutation récessive ; distribution ; influence de la température
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Abstract In Cuba, during its larval development inside its host egg,Trichogramma fuentesi turns its host egg either black or brown. Crossings carried out between the two forms ofT. fuentesi allowed to show that this character is determined by one locus with two alleles: Bh, dominant, colours the host black; and bh, recessive, colours the host brown. The bh allele is more frequent in the eastern region of Cuba which is also the warmest, than in the western region. We studied the evolution of frequencies of the two alleles in different experimental polymorphic populations at 23 °C and at 30 °C. In all tested conditions, bh was at a disadvantage by comparison with Bh, except at 30 °C when its initial frequency was high (90%). Thus, temperature alone should certainly not explain the maintenance of the observed polymorphism in Cuba.
    Notes: Résumé A Cuba, les oeufs hôtes parasités parTrichogramma fuentesi Torre (Hymenoptera: Trichogrammatidae) deviennent soit noirs, soit marrons. Une série de croisements entre Trichogrammes a permis de montrer que le déterminisme de ce caractère est sous la dépendance d'un locus portant deux allèles: Bh, dominant, confère une couleur noire à l'hôte; bh, récessif, confère une couleur marron. L'allèle bh étant plus fréquent dans la région orientale de Cuba, la plus chaude, nous avons suivi l'évolution des fréquences des deux allèles dans des populations expérimentales polymorphes placées à 23 °C et 30 °C. Dans toutes les conditions testées, bh a été désavantagé par rapport à Bh, sauf à 30 °C lorsque sa fréquence initiale était élevée (90%). La température n'explique donc certainement pas à elle seule le maintien du polymorphisme à Cuba.
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  • 8
    ISSN: 1572-8889
    Keywords: parasitoid ; foraging behavior ; Hymenoptera ; Cotesia ; Lepidoptera ; Pieris ; host location ; solitary ; gregarious ; specialist ; generalist
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the present study we apply a comparative approach, in combination with experimentation, to study behavior of two parasitoid species that attack caterpillar hosts with different feeding strategies (gregarious or solitary). In a semifield setup, consisting of clean cabbage plants and plants infested with one of two host species, the foraging behavior of the specialistCotesia rubecula, on obligate parasitoid of solitarily feedingPieris rapae larvae, was compared to that of the generalistCotesia glomerata, a polyphagous parasitoid of several Pieridae species (mainly the gregariously feedingPieris brassicae).Cotesia glomerata displayed equal propensity to search for and parasitize larvae of both host species. AlthoughC. glomerata exhibited a relatively plastic foraging behavior in that it searched differently under different host distribution conditions, its behavior seems more adapted to search for gregariously feeding hosts. Females exhibited a clear “area-restricted” search pattern and were more successful in finding the gregariously feeding caterpillars.Cotesia rubecula showed a higher propensity to search forP. rapae than forP. brassicae, i.e., females left the foraging setup significantly earlier when their natural hostP. rapae was not present.C. rubecula showed a more fixed foraging behavior, which seems adapted to foraging for solitarily feeding host larvae. In a setup with onlyP. rapae larvae, the foraging strategies of the two parasitoid species were quite similar. In a choice situationC. glomerata did not show a preference for one of the host species, whileCotesia rubecula showed a clear preference for its natural host species. The latter was shown by several behavioral parameters such as the number of first landings, allocation of search time, and percentage parasitization.
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  • 9
    ISSN: 1572-8889
    Keywords: host selection ; experience ; learning ; extinction ; reinforcement ; parasitoids ; Drosophila ; Leptopilina heterotoma ; Hymenoptera ; Eucoilidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The host-foraging behavior of female entomophagous parasitoids is commonly modified by positive associative learning. Typically, a rewarding experience (e.g., successful oviposition in a host) increases a female's foraging effort in a host microhabitat of the type associated with that experience. Less well understood are the effects of unrewarding experiences (i.e., unsuccessful foraging). The influence of unrewarding experience on microhabitat choice and residence time within a microhabitat was examined for the eucoilid parasitoid,Leptopilina heterotoma, in laboratory and greenhouse assays. As determined previously, females which oviposited successfully in either of two microhabitat types (fermenting apple or decaying mushroom) strongly preferred to forage subsequently on that microhabitat type. However, failure to find hosts in the formerly rewarding microhabitat caused females to reverse their preference in favor of a novel microhabitat type. The effect, though striking, was transient: within 1–2 h, the original learned preference was nearly fully restored. Similar effects of unrewarding experiences were observed with respect to the length of time spent foraging in a microhabitat. As determined previously, oviposition experience in a particular microhabitat type increased the time spent foraging in a patch of that microhabitat type. However, failure to find hosts in the patch caused the time a wasp spent in the next unoccupied patch of that type to decrease to almost nothing. In addition, there was a tendency for an unrewarding experience on a formerly rewarding microhabitat type to extend the time spent in a patch of a novel type. The function of the observed effects of unrewarding experiences is discussed.
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  • 10
    ISSN: 1572-8889
    Keywords: Hymenoptera ; Vespidae ; Vespula spp. ; foraging behavior ; chemoorientation ; social facilitation ; local enhancement ; spatial dispersion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A series of experiments on the discovery of meat bait by scavenging workers ofV. germanica andV. maculifrons showed that workers oriented to meats by visual cues and olfactory cues. Scavening workers ofV. germanica andV. maculifrons responded positively to the sight of conspecifics at meat baits; i.e., meat with (confined) wasps received more visits than meat without wasps. This attraction to conspecific workers on meat baits provides evidence of local enhancement, in that the presence of conspecifics at meat bait directs other wasps to a particular spatial locality. Local enhancement by yellowjackets foraging for meat may contribute to the clumped distribution of worker populations noted in studies of workers captured in meat-baited traps. We hypothesize that local enhancement of foraging is a ubiquitous behavior in species of theV. vulgaris species group and may be one attribute explaining their ecological success.
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  • 11
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    Journal of comparative physiology 175 (1994), S. 363-369 
    ISSN: 1432-1351
    Keywords: Hymenoptera ; Bees ; Homing ; Landmark guidance ; Distance perception
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Bees and wasps acquire a visual representation of their nest's environment and use it to locate their nest when they return from foraging trips. This representation contains among other features cues to the distance of near-by landmarks. We worked with two species of ground-nesting bees, Lasioglossum malachurum (Hymenoptera: Halictidae), Dasypoda hirtipes (Hymenoptera: Melittidae) and asked which cues to landmark distance they use during homing. Bees learned to associate a single cylindrical landmark with their nest's location. We subsequently tested returning bees with landmarks of different sizes and thus introduced large discrepancies between the angular size of the landmark as seen from the nest during training and its distance from the nest. The bees' search behaviour and their choice of dummy nest entrances show that both species of ground-nesting bees consistently search for their nest at the learned distance from landmarks. The influence of the apparent size of landmarks on the bees' search and choice behaviour is comparatively weak. We suggest that the bees exploit cues derived from the apparent speed of the landmark's image at their retina for distance evaluation.
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  • 12
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    Nutrient cycling in agroecosystems 40 (1994), S. 243-250 
    ISSN: 1573-0867
    Keywords: Ailanthus excelsa ; biomass ; growth ; N and P fertilizers ; N content ; N uptake ; P content ; P update ; silvicultural efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted on a coarse sand soil having pH 8.8 and organic matter 0.06% in Indian arid region, to study the influence of N and P fertilizers on growth, biomass and nutrient content ofAilanthus excelsa, which is an important fodder species of arid and semi arid regions. Three levels of nitrogen (0, 9 and 18 g N tree−1 as Urea) and of phosphorus (0, 3 and 6 g P2O5 tree−1 as Single Superphosphate) in factorial combinations were taken in triplicate and the experiment was laid in Randomised Block Design. Application of 9 g N plant−1 improved tree height by 15 to 25%, collar circumference by 30 to 37% and crown diameter by 18 to 26% in the initial 3 years. Total biomass increase due to 9 g N plant−1 was 76% and 59%, respectively, after 1 and 2 years of planting. Application of 3 g P2O5 tree−1 increased tree height by 8 to 18% and collar circumference by 17 to 24% during initial three years, and total biomass by 70% at 1 year and 30% at 2 years of age. Combined application of 18 g N and 3 g P2O5 tree−1 (N18P3) was the best treatment which increased tree height by 49%, 85% and 35% and collar circumference by 56%, 10% and 11% at 1, 2, and 3 years of age, respectively. N18P3 treatment increased the total biomass by 181% at 1 year and 185% at 2 years of age. N and P applications improved considerably the branching of roots and root length and enhanced root biomass by 2 to 3 folds. N18P3 treatment increased the nitrogen uptake by 304% (4.02 g tree−1) at 1 year and 211% (42.56 g tree−1) at 2 years of age. The P uptake was maximum (290.4 mg tree−1) due to N18P3 treatment in 1 year old and 11.37 g tree−1 due to N9P6 treatment in 2 year old plantation.
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  • 13
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    Nutrient cycling in agroecosystems 39 (1994), S. 217-222 
    ISSN: 1573-0867
    Keywords: growth ; K accumulation ; K nutrition ; physiological efficiency ; soybean ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments were conducted during 1989 to 1991 on a loamy sand (Typic ustochrept) soil to study the K nutrition of soybean. Soybean responded significantly up to 50 kg K ha−1 when applied 1/2 of K at planting and 1/2 at flower initiation (two split) or applying 1/3 K at planting, 1/3 at flower initiation and 1/3 at pod development (three splits). Split application was more beneficial than applying full K at time of planting due to higher leaf area index, crop growth rate, chlorophyll content of fresh leaves, K accumulation in soybean and better agronomic and physiological efficiency of applied K. Agronomic efficiency, physiological efficiency and apparent recovery of K reduced as rate of applied K was increased from 50 to 75 kg ha−1. Highest K+ concentration (3.4 % of dry matter) was recorded in 30-day-old plants at 75 kg K ha−1 which depressed progressively with the age of the crop. At maturity, the K concentration of soybean seed varied from 1.5% (unfertilised K plants) to 2.1 % (when 75 kg K ha−1 was applied in three splits).
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  • 14
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    Journal of statistical physics 74 (1994), S. 91-109 
    ISSN: 1572-9613
    Keywords: Random process ; local interaction ; critical phenomena ; invariant distribution ; growth ; eroder ; convexity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Components which are placed in a finite or infinite space have integer numbers as possible states. They interact in a discrete time in a local deterministic way, in addition to which all the components' states are incremented at every time step by independent identically distributed random variables. We assume that the deterministic interaction function is translation-invariant and monotonic and that its values are between the minimum and the maximum of its arguments. Theorems 1 and 2 (based on propositions which we give in a separate Part II), give sufficient conditions for a system to have an invariant distribution or a bounded mean. Other statements, proved herein, provide background for them by giving conditions when a system has no invariant distribution or the mean of its components' states tends to infinity. All our main results use one and the same geometrical criterion.
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  • 15
    ISSN: 1420-9098
    Keywords: Hymenoptera ; social bees ; behavioural castes ; behaviour ; Exoneura bicolor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Exoneura bicolor is a univoltine allodapine bee common in montane forests of southern Australia, where it exhibits a semisocial/quasisocial colony organization. Within-nest behaviour in postemergence autumn nests ofExoneura bicolor was recorded with the aim of studying behavioural specialization in pre-reproductive colonies. Ten complete colonies were transferred to purpose-built observation nests shortly before brood eclosion in late summer. Behaviour within observation nests was recorded for periods of up to 44 days after establishment, covering a period when colonies are preparing for overwintering. Dispersal of females and brood rearing do not occur at this time, although some females may become inseminated. Analyses of data using multivariate techniques indicated four distinguishable behavioural castes, designated here as Guards, Nest Absenters, Nest Modifiers and Non-recruits. This represents a higher degree of behavioural specialization than recorded to date for other allodapines. Behaviours performed by Guards and Nest Absenters are likely to involve considerable risks, but benefit the colony as a whole, so that some nestmates in prereproductive colonies exhibit altruism that frequently aids adult siblings or cousins. The males in our study were fed by females via trophallaxis and two of the males participated in nest maintenance tasks. Our results suggest that autumn colonies ofE. bicolor form well-integrated behavioural units even though brood rearing does not commence until the following spring.
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  • 16
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    Aquatic sciences 56 (1994), S. 70-79 
    ISSN: 1420-9055
    Keywords: Macrophytes ; growth ; assimilation ; macroelements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Helophytes are often incorporated into biological wastewater treatment plants. In favourable situations, they can take up large amounts of nutrients. One helophyte, the reed canarygrass (Phalaris arundinacea L.), is fast growing when supplied with sufficient light and nutrients. Experiments were carried out under natural climatic conditions in small plastic tanks filled with sand with regular additions of a balanced and concentrated nutrient solution. In the growing season (May–October), plant production reached 10.5 kg m−2 of dry biomass, of which 66% was in the aerial parts. Maximum nutrient uptake capacity was reached just after flowering and before senescence (beginning of October): 49% N, 34% P, 52% K and 34% Mg of the input was fixed in the aerial parts, which are easily harvestable. The corresponding values for the below ground parts were 12%, 10%, 11% and 11% respectively. Excretion of K and Mg has been observed when nutrients are translocated to the storage organs.
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  • 17
    ISSN: 1423-0445
    Keywords: chemical communication ; queen cells ; brood pheromone ; brood recognition ; fatty acid esters ; Hymenoptera ; Apidae ; Apis mellifera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Honey bee workers are able to nurse or to destroy and thus to recognize the capped queen cells containing a pupa. Fatty acid esters, especially methyl oleate, methyl palmitate and ethyl oleate were found in significant amounts on the queen pupal cuticle. Methyl oleate, the major component, along with smaller amounts of methyl linoleate and methyl linolenate, were involved in the recognition of queen cells by workers. In natural conditions of the colony, queen cells containing a paraffin pupal lure with methyl oleate were accepted 5.9 days by workers, releasing about 1.8 queen pupa equivalents during that period, when control cells (without ester) were kept only 2.1 days. Although these esters are non specific to honey bees, they are of great importance in social regulation of the honey bee colony.
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  • 18
    ISSN: 1423-0445
    Keywords: predation ; plant-insect interactions ; tritrophic level interactions ; iridoid glycosides ; catalpol ; Lepidoptera ; Nymphalidae ; Junonia coenia ; Hymenoptera ; Formicidae ; Camponotus floridanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We investigated the role of the iridoid glycoside, catalpol, as a deterrent to the predator,Camponotus floridanus. Four laboratory colonies of this ant were offered buckeye caterpillars (Junonia coenia: Nymphalidae) raised on diets with and without catalpol. The same colonies were offered sugar-water solutions containing varying concentrations of catalpol, in both no-choice and choice tests. Regardless of diet, buckeye caterpillars appeared to be morphologically protected from predation by the ants, possibly because of their large spines or tough cuticle. However, buckeyes raised on diets with catalpol had high concentrations of catalpol in their hemolymph; extracts of this high-catalpol hemolymph proved to be an effective deterrent to the ants. When starved ants were not given the choice of food items, they were more likely to consume sucrose solutions that contained 5 mg catalpol/ml or 10 mg catalpol/ml than they were to consume solutions with 20 mg catalpol/ml. When they were given a choice of sugar solution or a sugar solution containing catalpol, the ants avoided solutions with catalpol at any of these concentrations. Ant colony responses to catalpol in sucrose solutions varied considerably over time and among colonies.
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  • 19
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    Development genes and evolution 203 (1994), S. 450-453 
    ISSN: 1432-041X
    Keywords: Athalia rosae ; Hymenoptera ; Sperm injection ; Parthenogenesis ; Chimera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mature unfertilized eggs (oocytes) dissected from the ovary of the sawfly Athalia rosae (Hymenoptera) begin parthenogenetic development if exposed to distilled water and produce haploid males. Injection of sperm into mature oocytes through the anterior pole resulted in karyogamy in a fraction of cases which developed as diploid females. No haploid-haploid chimeras due to independent participation of the injected sperm in development were produced. When sperm were injected through the posterior pole, however, fertilization never occurred but haploid-haploid chimeras were produced in a smaller fraction of cases. Both egg nucleus-derived and injected sperm-derived nuclei contributed in forming the germ cells of the chimeric males.
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  • 20
    ISSN: 1573-1561
    Keywords: Pheromone ; 3,5-dimethyl-6-(methylethyl)-3,4,5,6-tetrahydropyran-2-one ; enantiomer ; parasitoid ; Macrocentrus grandii ; Hymenoptera ; Braconidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In a previous study we reported identification of (3R*,5S*,6R*)-3,5-dimethyl-6-(methylethyl)-3,4,5,6-tetrahydropyran-2-one as a component of the pheromone ofMacrocentrus grandii Goidanich. The lactone was present in male and female wasps, and laboratory and field bioassays demonstrated that both sources of the lactone elicit flight initiation, upwind anemotaxis, and casting in male wasps. In the present study, the synthetic (3R,5S,6R)- and (3S,5R,6S)-lactone enantiomers (RSR andSRS, respectively) were bioassayed for biological activity. In wind tunnel studies theSRS enantiomer elicited flight initiation, upwind anemotaxis, and casting by male wasps comparable to lactone derived from male and female wasps. Flight response to theRSR enantiomer averaged 14 percent of theSRS enantiomer. No specific ratio of the stereoisomers was found more attractive than theSRS enantiomer alone. Field studies demonstrated theSRS enantiomer was active alone in attracting male wasps. When paired with (Z)-4-tridecenal (a previously identified female-derived sex pheromone), theSRS enantiomer yielded a synergistic response comparable to (Z)-4-tridecenal plus female-derived lactone.
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  • 21
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    Journal of chemical ecology 20 (1994), S. 2307-2321 
    ISSN: 1573-1561
    Keywords: Polistes metricus ; Hymenoptera ; Vespidae ; cuticular hydrocarbons ; discriminant analysis ; nestmate recognition ; social wasps
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The cuticular lipids ofPolistes metricus queens, workers and males from seven laboratory-maintained colonies were extracted and analyzed by combined gas chromatography-mass spectrometry. Males had higher proportions of alkenes (20.5%) in their cuticular lipids than did queens (2.3%) or workers (7.7%). Discriminant analyses of the cuticular lipid profiles of the adult wasps showed that males group separately from females. Additional analyses showed that queens group with their respective workers by colony and that queens group even more closely with males by colony. The most distinct groupings occurred with workers only by colony and with males only by colony. Stepwise discriminant analyses showed that each type of grouping was dependent upon a different combination of cuticular lipids.
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  • 22
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    Journal of chemical ecology 20 (1994), S. 2437-2453 
    ISSN: 1573-1561
    Keywords: Varroa jacobsoni ; Acari ; Varroidae ; mite ; Apis mellifera ; Hymenoptera ; Apidae ; honeybee ; chemoreception ; host selection ; cuticle ; hydrocarbons ; alkanes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ectoparasitic miteVarroa jacobsoni invades worker brood cells of the honeybeeApis mellifera during the last 20 hr before the cells are sealed with a wax cap. Cuticle extracts of 8-day-old worker honeybee larvae occupying such brood cells have an arrestment effect on the mite. The mites run for prolonged periods on the extract, systematically returning onto the stimulus after touching the borders of the treated area. Mites increase walking speed and path straightness in response to increasing doses of a nonpolar fraction of the cuticle extract. Saturated straight-chain odd-numbered C19–C29 hydrocarbons were identified by thin-layer argentation chromatography and gas chromatography-mass spectrometry as the most active constituents, with branched alkanes also contributing to the arrestment effect of this active fraction. Analysis of the behavior responses to syntheticn-alkanes indicate that the response is probably based on a synergism between the different alkane components of the fraction rather than to an individual compound.
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  • 23
    ISSN: 1573-1561
    Keywords: Herbivory ; host preference ; host selection ; oviposition ; phenolic glucosides ; willow ; Salicaceae ; Salix ; galling sawfly ; Hymenoptera ; Tenthredinidae ; Euura amerinae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effects of phenolic glucosides on the oviposition behavior ofEuura amerinae L. (Hymenoptera: Tenthredinidae) were tested in multiple oviposition experiments using different shoot length categories ofSalix pentandra L. (with different amounts of phenolic glucosides) and in experiments with pure phenolic glucosides (salidroside, arbutin, salicin, 90% salicortin, 90% 2′-O-acetylsalicortin) or composite total fractions of phenolic glucosides from three willow species (S. pentandra, S. myrsinifolia Salisb.,S. triandra L.). This was the first time that the effects of pure phenolic glucosides on the oviposition behavior of sawfly species were tested. Total fraction of phenolic glucosides fromS. pentandra and its main individual glucoside, 2′-O-acetyl-salicortin, stimulated the strongest ovipositional behavior inE. amerinae. The results show clearly that females ofE. amerinae can recognize and choose their host willow,S. pentandra, on the basis of phenolic glucosides. Moreover, they are probably able to use phenolic glucosides as a cue in shoot selection within host-plant individuals.
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  • 24
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    Journal of sol gel science and technology 2 (1994), S. 97-101 
    ISSN: 1573-4846
    Keywords: hybrid materials ; monodispersed particles ; growth ; seeds ; LCD spacer
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    Topics: Chemistry and Pharmacology
    Notes: Abstract This paper describes the attempt to prepare a new group of monodispersed silica-polymer hybrid particles, which consists of silica and amide polymer: poly(vinylpyrrolidone) (PVP) and poly(2-ethyloxazoline) (POXZ). Preparation method is based on the growth of hybrid seeds by the addition of TEOS-polymer solution. Monodispersed PVP-silica hybrid particles of 1.24 µm in diameter were prepared by growing the hybrid seeds of 0.54 µm by the addition of TEOS-PVP solution with ammonia catalyst. In the case of POXZ-silica particles, addition of TEOS-POXZ solution to the solution containing 0.54 µm seeds resulted in monodispersed POXZ-silica hybrid particles and four times repetition of the addition for particle growth gave the hybrid particles of the diameter of 1.6 µm. Improvement of mechanical properties of hybrid particles was observed when the particles were heated at 100 ∼ 200°C.
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  • 25
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    Journal of sol gel science and technology 2 (1994), S. 273-276 
    ISSN: 1573-4846
    Keywords: aging in silica gels ; growth ; aggregation ; X-ray scattering
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Growth and aging of silica aggregates are influenced both by temperature and by catalyzing fluorine ions as shown by SAXS and BET. It was found that both fluorine and increased temperature slightly increased the fractal dimension Df during aging, but the fluorine catalyzed system showed a lower BET surface area. To understand the effect of fluorine and increased temperature on the aggregates, 2D aggregations and SAXS simulations were carried out using two new programs GRASP and DALAI. In agreement with experiments it was found that binary RLCCA aggregates have a slightly higher Df value compared to DLCCA aggregates and that branch-flexibility during aging increases Df even further.
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  • 26
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    Plant molecular biology 26 (1994), S. 1529-1555 
    ISSN: 1573-5028
    Keywords: gibberellin ; growth ; development ; perception ; receptor ; gene expression ; signal transduction ; response mutant ; calcium
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  • 27
    ISSN: 1432-0878
    Keywords: Key words: Microvascular endothelial cells ; Cell ; growth ; Extracellular matrix ; Human
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    Topics: Biology , Medicine
    Notes: Abstract. We introduce two methods, both of which are based on cellular-extracellular matrix interaction, which will facilitate the study of human microvascular endothelial cells. One method describes the means to obtain a G1 population baseline in human microvasclular endothelial cells. Because of the contribution of the extracellular matrix in endothelial cell growth, synchronization in G1 was possible only after the incorporation of angiostatic levels of heparin and hydrocortisone into the extracellular matrix. In the second method, we demonstrate that selective perturbation of human microvascular endothelial cell-extracellular matrix interactions results in the induction of a transitional growth state, between proliferative and differentiated growth states, in human microvascular endothelial cells. In the functional, microtubule formation assays, transitional growth state endothelial cells display rates that are indermediate between those obtained from differentiated and proliferative endothelial cells. Our results demonstrate the importance of the human microvascular endothelial cell-extracellular matrix interaction in the determination of cellular growth state. Our findings also imply that responsiveness of microvascular endothelial cells to their cellular-extracellular matrix environs is highest during the differentiated growth state.
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  • 28
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    Potato research 37 (1994), S. 365-371 
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; multiplication ; micropropagation ; growth ; leaf removal ; tissue culture variation
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Removing the leaves from single node potato cuttings in vitro allows propagules to be placed in culture vessels at increased densities with the intent of saving resources and space. Under light intensities of 22 wattsm−2, the removal of the subtending leaf resulted in fewer nodes, shorter plantlets, smaller leaf area, and lower fresh and dry weights of 4-week-old plantlets of cvs Atlantic, Kennebec, Russet Burbank and Shepody. Fewer nodes and reduced plantlet height may result in inefficient multiplication protocols because fewer propagules are available and shorter internodes make dissection more difficult. The coefficient of variation for the various growth parameters was greater when propagules lacked leaves, indicating that the variability of the plant material for propagation was increased. A reduction of vigour and growth was still evident when plantlets of cv. Shepody were grown for 6–8 weeks.
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  • 29
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    Aquatic ecology 28 (1994), S. 453-458 
    ISSN: 1573-5125
    Keywords: filter-feeding ; energy cost ; growth
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    Topics: Biology
    Notes: Abstract An overview of recent findings concerning filter-feeding inNereis diversicolor is given. It has been discovered that the facultative filter-feederN. diversicolor may meet its metabolic requirements on a pure diet of phytoplankton, just as a typical obligate filter-feeder. Consequently, this worm is likely to be a hitherto undervalued key organism in the control of phytoplankton production in many shallow brackish water areas.
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  • 30
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    Journal of applied phycology 6 (1994), S. 41-44 
    ISSN: 1573-5176
    Keywords: Prorocentrum ; semi-continuous culture ; okadaic acid ; growth ; suspension culture
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    Topics: Biology
    Notes: Abstract Clonal cultures ofProrocentrum hoffmannianum Faust (clone 882a) were grown under optimal environmental conditions for maximal okadaic acid production. The environmental conditions of 25 °C and 86 µmol photon m-2 s-1 were used to cultivateP. hoffmannianum in a semi-continuous 36-L culture vessel with continuous cell suspension and pH control. Using these conditions, a 3-fold increase in harvestable biomass and okadaic acid content was observed when compared to batch culture techniques.
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  • 31
    ISSN: 1573-5176
    Keywords: Dunaliella viridis ; subsidiary energy quantification ; growth ; carotenoids
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    Topics: Biology
    Notes: Abstract An account is given of the influence of different levels of mechanical energy, in the form of bubbling, on the growth of the microalgaDunaliella viridis when other variables (e.g. temperature, nutrient supply, photon fluence) do not change. The extra energy was quantified accurately through the application of the classical equations of mass and energy conservation providing a method for the calculation of the energy efficiency of primary production related to the total energy input, in which photon fluence was found to be the most important. The specific growth rate (μ) of the population vs the input of auxiliary energy fits to a second order polynomial function with a maximum growth rate at 0.63 W m−2. The increase of maximal cell density follows a hyperbolic saturation kinetics, with saturation at those same values of extra energy. Both primary production and the efficiency of energy transformation inD. viridis vs the variation of total energy input fit to hyperbolic functions, reaching a maximum efficiency for primary production of 0.85%.
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  • 32
    ISSN: 1573-5176
    Keywords: deep seawater ; micro-algae ; growth ; yield
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    Topics: Biology
    Notes: Abstract Deep seawater (DSW; seawater under the euphotic layer), obtained from Deep Seawater Laboratory in Muroto, Japan, was applied to the culture ofDunaliella tertiolecta, D. salina, Nannochloropsis oculata, N. salina, Porphyridium cruentum, Tetraselmis tetrahele, andChaetoceros ceratosporum. DSW supported the exponential growth of every species. The growth yields were at 14.7 (±2.3 SD) mg dry weight per liter, and could be heightened by the addition of nitrate to DSW.
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  • 33
    ISSN: 1573-5168
    Keywords: Atlantic salmon ; turbot ; cell culture ; salinity ; growth ; lipids
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    Topics: Biology
    Notes: Abstract The direct effects of osmotic pressure (salinity) on growth performance and lipid composition were investigated in fish cells in culture. Cell lines from a relatively stenohaline marine species, turbot (Scophthalmus maximus) (TF) and an anadromous species, Atlantic salmon (AS) were cultured in media supplemented with NaCl to produce osmotic pressures varying from 300 to 500 mOsm kg−1. The growth rates of the two cell lines were affected in a similar manner by the salinity of the media with the rank order for both peak cell numbers and growth rates up to the day of peak cell number being 300 〉 350 〉 400 〉 450 〉 500 mOsm kg−1. Cell death occurred in both cell lines in older cultures at all salinities with the greatest loss of viable cells in media of 300 and 350 kg−1. However, there were quantitative and qualitative differences between the cell lines in their lipid metabolism in response to the salinity of the media. The lipid content expressed per cell showed a positive correlation between lipid per cell and salinity in TF cells, but this was less apparent in AS cells. The percentage of total polar lipid classes increased with increasing salinity in TF cells due mainly to graded increases in the percentages of choline phospholipids. In contrast, there were no significant differences in the proportions of polar and neutral lipid classes with salinity in AS cells. The only significant effect of salinity in AS cells was a decreased proportion of dimethylacetals in total lipid at the highest salinity. The same significant effect of salinity on dimethylacetal content of total lipid was observed in TF cells. However, in addition there was a graded decrease in the percentage of 18:2n-9 in TF cell total lipid with increasing salinity. This was accompanied by increased percentages of total n-3 and n-6 PUFA with higher proportions of both groups of PUFA at 450 and 500 compared with 300 mOsm kg−1. The results show that environmental salinity, in the absence of hormonal or other physiological stimuli, has direct effects on the growth and lipid metabolism of fish cells and that these effects differ in cells from different fish species.
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  • 34
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    Plant systematics and evolution 193 (1994), S. 173-186 
    ISSN: 1615-6110
    Keywords: Rhamnaceae ; Frangula ; Diptera ; Coleoptera ; Hymenoptera ; Mecoptera ; Breeding system ; phenology ; pollination biology ; protandry ; reproductive success ; self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Various aspects of the reproductive biology of the perennial hermaphroditeFrangula alnus Mill. were studied in two populations located in the province of Cádiz, southern Spain. Flowering extends from the second week of May to early July. The small, whitegreenish, entomophilous flowers are incompletely protandrous and last 8–10 days, but pollen transfer takes place only on days 1–3. A very weak, pleasant odour along with nectar and pollen attract over 45 insect species, of which 21 are probable pollinators. These are mainlyDiptera but also includeHymenoptera and the unusual (as flower visitors)Mecoptera. Reproduction is exclusively sexual and strictly xenogamous, with pollen transfer depending solely on insect vectors. Although flower morphology and individual flower phenology do not fully prevent self-pollination, and geitonogamy can easily take place, the level of autogamy was negligible. Therefore, some self-incompatibility mechanism is operative in this species. Only 2.8% of open-pollinated flowers set fruit. At the flower level, fruit initiation was apparently limited by availability of cross-pollen, which in turn seemed influenced by the structure of the population. The seed/ovule ratio in ripe fruits was ca. 50%. Predispersal (maternal) reproductive success (percent ovules becoming filled seeds) was 1.42%. For mature individuals this corresponded to ca. 430 to 1560 potential offspring per year.
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  • 35
    ISSN: 1573-5036
    Keywords: biomass allocation ; carbon isotope discrimination ; growth ; water use efficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants of two bread wheat cultivars,Triticum aestivum L. ‘Katya Al’ and ‘Mexipak 65’, were grown in pots during the crop season in the field in NW Syria, a region with a Mediterranean climate. The experiment involved two treatments. Control plants were well-watered throughout the experiment (watering to 0.22 g water g−1 dry soil). In the second treatment, water was withheld from the plants until soil water content had decreased to 0.10 g water g−1 dry soil, the level that was maintained thereafter. Water use was measured by weighing the pots, and growth by destructive sampling. Growth of Katya and Mexipak was similar. Mexipak had a lower (though not significant atp〈0.05) plant water use efficiency (WUEp) in both treatments due to higher rates of water loss. On a leaf area basis differences in water use were especially high since Mexipak had a smaller total leaf area. In spite of a smaller investment in photosynthesizing area, Mexipak achieved similar growth as Katya. Carbon isotope discrimination and organic nitrogen concentration (both higher for Mexipak) suggest that Mexipak accomplished higher mean net photosynthetic rates with a higher mean leaf diffusive conductance, higher intercellular carbon dioxide partial pressure, and possibly a greater investment in the photosynthetic apparatus compared to Katya. Differences in carbon isotope discrimination suggest a larger difference in average photosynthetic WUE (net photosynthesis/transpiration) than in plant WUE. This could indicate that loss of carbon in respiration was greater in Katya. Gas exchange measurements on the youngest fully expanded leaves showed only minor differences between the cultivars. It is hypothesized that Mexipak, with a smaller total leaf area, is able to maintain high leaf conductance and photosynthesis for a longer period of time during the day or during the life span of leaves.
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  • 36
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    Plant and soil 165 (1994), S. 315-321 
    ISSN: 1573-5036
    Keywords: cell wal's ; epidermis ; growth ; root development ; soil penetration ; stiffness ; Zea diploperennis ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The development of the epidermal layer of roots of Zea is traced from the quiescent centre to the zone where root hairs develop. In the zone of cell division a three layered coat forms on the outside of the epidermal cells consisting of the outer epidermal walls, overlaid by a two-layered pellicle composed of a thick fibrillar inner layer of polysaccharide, and a thin fibrillar outer layer of protein. The epidermal cells divide several times in the same longitudinal file but rarely across a radius to give a new longitudinal file. Thus, the radial walls become much thicker than all but the original transverse walls, and packets of up to 32 daughter cells derived from a single initial may be distinguished. The pellicle develops during these divisions as a continuum over the outer walls of the daughter cells. It is proposed that the pellicle provides a stiffening to the forward end of the root which permits it to penetrate soil without bending. Support for this hypothesis is shown by the Zea mays mutant Ageotropic in which the pellicle is absent, the epidermal surface is disorganized, and which grows crookedly through soil. In the zone of extension growth of normal roots of two Zea species the pellicle thins and disappears. Circumferential strips of the pellicle were peeled off the young epidermal cells and could be stretched to twice their length. This deformation is partly the result of the pellicle stretching and breaking above the attachments of the radial walls. After normal thinning of the pellicle, detachment of the radial walls at their outer ends produces a corrugated surface in the proximal zone of the root tips. In dicotyledons (e.g., soybean), there is no similar pellicle, but a stiff root tip is produced by a long multi-layered root cap, the proximal portion of which covers the elongating epidermal surface.
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  • 37
    ISSN: 1573-5036
    Keywords: growth ; osmotic adjustment ; saline stress ; solutes ; Lycopersicon esculentum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of a short period of saline stress was studied in two phenotypically different cultivars, one of normal fruit-size (L. esculentum cv. New Yorker) and one of cherry fruit-size (L. esculentum var.cerasiforme cv. PE-62). In both cultivars the relative growth rate (RGR) and the leaf area ratio (LAR) decreased following salinisation. The leaf turgor potential (ψp) and the osmotic potential at full turgor (ψos) decreased to the same extent in both cultivars. However, the contributions of organic and inorganic solutes to the osmotic adjustment was different between cultivars. New Yorker achieved the osmotic adjustment by means of the Cl− and Na+ uptake from the substrate, and by synthesis of organic solutes. In the cherry cultivar organic solutes did not contribute to the osmotic adjustment, instead, their contribution decreased after salinisation. After the salt stress was removed, the water stress disappeared, the content of organic solutes decreased in plants of both cultivars and, therefore, their growth was not retarded by the diversion of resources for the synthesis of organic solutes. However, the toxic effects of the Cl− and Na+ did not disappear after removal of the salt stress, and the net assimilation rate (NAR) and the rate of growth (RGR) did not recover.
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  • 38
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    Plant and soil 167 (1994), S. 43-49 
    ISSN: 1573-5036
    Keywords: DNA contents ; DNA synthesis ; growth ; proliferation ; radicle ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The pattern of proliferation and growth of cortical and central metaxylem cells in a radicle and the transitional zone of a wheat embryo was studied during the final stages of embryogenesis. Cell divisions finished nearer the root tip in the central metaxylem than was the case in the cortex. After divisions ceased the cells of both tissues maintained the ability to synthesize DNA and the cells began DNA endoreduplication. The maximal levels of endoreduplication were 4C and 8C in cortical and central metaxylem cells, respectively. As a result of nonsimultaneous cessation of divisions, the metaxylem cells were two or three times longer than cortical cells. The proportion of cells with the maximal DNA content was smaller in the transitional zone than in the radicle. During the final embryonal stages cell growth rate was decreased. It was established that the transition of cells to DNA synthesis was inhibited in all sites of the radicle during the completion of embryogenesis. The cell growth was topped in proximal sites of the radicle. In the division zone the cells which had already begun DNA synthesis were able to complete it and divided. Cell growth stopped simultaneously with completion of proliferation in this zone.
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  • 39
    ISSN: 1573-5036
    Keywords: Climate change ; elevated carbon dioxide ; growth ; Laccaria laccata ; mycorrhization ; oak ; Quercus robur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pedunculate oak (Quercus robur L.) was germinated and grown at ambient CO2 level and 650 ppmv CO2 in the presence and absence of the ectomycorrhizal fungus Laccaria laccata for a total of 6 month under nutrient non-limiting conditions. Mycorrhization and elevated atmospheric CO2 each supported the growth of the trees. Stem height, stem diameter, and dry matter accumulation of pedunculate oak were increased by mycorrhization. Elevated atmospheric CO2 enhanced stem height, stem diameter, fresh weight and dry weight, as well as lateral root formation of the trees. In combination, mycorrhization and elevated atmospheric CO2 had a more than additive, positive effect on tree height and biomass accumulation, and further improved lateral root formation of the trees. From these findings it is suggested that the efficiency of the roots in supporting the growth of the shoot is increased in mycorrhized oak trees at elevated atmospheric CO2.
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  • 40
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    Plant and soil 164 (1994), S. 169-176 
    ISSN: 1573-5036
    Keywords: branching ; growth ; lateral roots ; maize ; root morphology ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The architecture of the root system is related to its water and mineral uptake. In this paper, the number, growth, and branching of first-order lateral roots are studied on field grown maize (early maturing cultivar ‘Dea’), mainly in relation to the depth and to the rank of the bearing phytomer. The soil was a deep clay loam, without any barrier until 1.80 m. The branching density was studied along axile roots until 1.40 m from the base, on a sample of individually excavated axile roots. A strong gradient of density was shown: the mean branching density decreased from 12 roots.cm−1 near the base to 4 roots.cm−1 at a 60 cm depth. Seminal roots were less densely branched than nodal roots. The mean difference was about 4 roots.cm−1. The length and branching density of lateral roots were studied on mature parts of the root systems where the growth and branching of the laterals were completed, using samples extracted from large soil monoliths. The length distribution of lateral roots was highly asymmetrical, for every source phytomer (mean: 25 mm; median: 16 mm). Many lateral roots were very short, and only 2 % reached a length higher than 10 cm. Only 29 % of all the laterals bore second-order lateral roots. Vigorous laterals branched more systematically and more profusely: the branching density varied from 2 to 5 roots.cm−1 according to the length of the mother lateral root. Both the number and length of lateral roots appeared to be affected by the soil bulk density which varied with the depth.
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  • 41
    ISSN: 1573-5036
    Keywords: Casuarina ; growth ; macronutrients ; sodicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A greenhouse experiment was conducted to evaluate the effect of increasing exchangeable sodium percentage (ESP)viz. 11, 17, 35, 58 and 70 on the growth and the concentration of important macronutrients in needles and stems of Casuarina obesa Miq., C. glauca Sieb. ex. spreng., C. cunninghamiana Miq. and C. equisetifolia Forster and G. Forster. A significant increase in the dry matter was found with increasing soil sodicity. At ESP 70, the percentage increase in dry matter over ESP 11 for the first three species was 44%, 35% and 124% in the needles, and 78%, 37% and 86% in stems, respectively. On the other hand the yields of C. equisetifolia decreased with increase in soil sodicity. Among the 4 species, C. obesa and C. glauca produced comparatively more DM under sodicity than did C. cunninghamiana and C. equisetifolia. The effect of sodicity was more pronounced on needles than on stems. Sodium concentration in plant parts increased with increasing sodicity, whereas concentration of potassium, calcium, magnesium, nitrogen and phosphorus showed a decrease.
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  • 42
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    Photosynthesis research 39 (1994), S. 303-320 
    ISSN: 1573-5079
    Keywords: phytoplankton ; primary production ; growth ; action spectrum ; biogeochemical cycling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Concerns about stratospheric ozone depletion have stimulated interest in the effects of UVB radiation (280–320 nm) on marine phytoplankton. Research has shown that phytoplankton photosynthesis can be severely inhibited by surface irradiance and that much of the effect is due to UV radiation. Quantitative generalization of these results requires a biological weighting function (BWF) to quantify UV exposure appropriately. Different methods have been employed to infer the general shape of the BWF for photoinhibition in natural phytoplankton, and recently, detailed BWFs have been determined for phytoplankton cultures and natural samples. Results show that although UVB photons are more damaging than UVA (320–400 nm), the greater fluxes of UVA in the ocean cause more UV inhibition. Models can be used to analyze the sensitivity of water column productivity to UVB and ozone depletion. Assumptions about linearity and time-dependence strongly influence the extrapolation of results. Laboratory measurements suggest that UV inhibition can reach a steady-state consistent with a balance between damage and recovery processes, leading to a non-linear relationship between weighted fluence rate and inhibition. More testing for natural phytoplankton is required, however. The relationship between photoinhibition of photosynthesis and decreases in growth rate is poorly understood, so long-term effects of ozone depletion are hard to predict. However, the wide variety of sensitivities between species suggests that some changes in species composition are likely. Predicted effects of ozone depletion on marine photosynthesis cannot be equated to changes in carbon flux between the atmosphere and ocean. Nonetheless, properly designed studies on the effects of UVB can help identify which physiological and ecological processes are most likely to dominate the responses of marine ecosystems to ozone depletion.
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  • 43
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    Photosynthesis research 39 (1994), S. 389-400 
    ISSN: 1573-5079
    Keywords: elevated CO2 ; nitrogen supply ; photosynthesis ; acclimation ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A common observation in plants grown in elevated CO2 concentration is that the rate of photosynthesis is lower than expected from the dependence of photosynthesis upon CO2 concentration in single leaves of plants grown at present CO2 concentration. Furthermore, it has been suggested that this apparent down regulation of photosynthesis may be larger in leaves of plants at low nitrogen supply than at higher nitrogen supply. However, the available data are rather limited and contradictory. In this paper, particular attention is drawn to the way in which whole plant growth response to N supply constitutes a variable sink strength for carbohydrate usage and how this may affect photosynthesis. The need for further studies of the acclimation of photosynthesis at elevated CO2 in leaves of plants whose N supply has resulted in well-defined growth rate and sink activity is emphasised, and brief consideration is made of how this might be achieved.
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  • 44
    ISSN: 1573-5117
    Keywords: Ruppia drepanensis ; ammonia toxicity ; temperature effects ; photosynthesis ; growth
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    Topics: Biology
    Notes: Abstract In a laboratory experiment, Ruppia drepanensis Tineo seedlings from a brackish marsh in Southern Spain were grown at 20 and 30 °C, at three different nitrogen levels. These levels were obtained by the addition of a slow release fertilizer (23% NH4NO3 by weight) to a sediment mixture of sand and clay (3:1). Several morphometric parameters were recorded during the first five weeks of the experiment, and photosynthesis and respiration were measured after 7 weeks of growth. Results showed a significant reduction of growth and development with increasing nitrogen and temperature levels. Dark respiration increased strongly at high nitrogen levels. At the same time, net photosynthesis at 250 and 500 µE m-2 s-1, Pm, Km and LCP were not affected by either factor. We attribute these phenomena to ammonia toxicity, since relatively high total ammonia (NH3 + NHf4 p+) levels were found in the interstitial water.
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  • 45
    ISSN: 1573-5117
    Keywords: mussel ; mussel culture ; growth ; standing stock ; food ; carrying capacity ; hydrodynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To evaluate the effects of a large scale coastal engineering project on the mussel (Mytilus edulis) bottom culture in the Oosterschelde estuary (S.W. Netherlands), mussel growth and production in the period 1980–1990 are studied in relation to food supply and the hydrodynamic conditions. Due to the construction of a storm-surge barrier and two additional dams, the risk that mussels are swept away by high current velocities decreased, resulting in an increase of the area in the Oosterschelde potentially suitable for mussel culture and in food availability now being more important as a limiting factor. For the Oosterschelde, a clear relation between mussel growth, stock sizes, and phytoplankton dynamics has been demonstrated. The meat yield of mussels landed in autumn — which is an index for growth rate — seems to be determined by the phytoplankton production in the preceding summer. In years with dense bivalve stocks, phytoplankton production and meat yields are relatively low. It is concluded that an increase of the mussel biomass cultured can result in a reduction of the primary production and, consequently, in a deterioration of the growing conditions for suspension-feeders in the estuary. This conclusion is supported by model calculations. An expansion of mussel culture in the new Oosterschelde is therefore dissuaded. Apart from primary production and stock sizes, food supply for mussels on culture lots appeared to be controlled by the horizontal advection of phytoplankton between and within the tidal channels. An observed decline in mussel landings from certain areas is attributed to the reduced mixing energy of the estuary in relation to the present distribution of the lots over the estuary. Production figures from the experimental lots, established in 1988 in the newly available areas, demonstrate that the yield of mussels can be enhanced by relaying culture lots towards the areas where the phytoplankton is produced. It is expected that by redistributing the culture lots, without expanding the biomass cultured, the carrying capacity of the Oosterschelde for mussel culture can be maintained.
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  • 46
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    Hydrobiologia 272 (1994), S. 125-146 
    ISSN: 1573-5117
    Keywords: Moina ; tropical ponds ; growth ; fecundity ; production ; demography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sampling and experiments were performed in brackish fishponds, located near the Ebrie Lagoon, Ivory Coast. Moina developed here at salinities up to about 4 g l-1. Mean embryonic and juvenile development times in experimental conditions were 0.82 and 0.80 day, respectively, with 2 juvenile stages at 30 °C (salinity of 3 g l-1; 29 µg chlorophyll-a l-1) and 1.22 and 1.37, with 2 stages for most individuals and 3 or 4 stages for some of them at 26 °C (2 g l-1 and 36 µg l-1). The corresponding Q10 for embryonic development was relatively high (2.7). Growth rates in weight at 30 ° C were 2.11 and 1.37 µg µg-1 d-1, respectively, for the embryonic and juvenile stages, 0.21 for mean somatic growth of the five first female adult stages, and 1.00 for primipara, summed for somatic growth, production of eggs, and embryonic growth. Consequently, differences in growth rates between young and adults were small. M. micrura also was a high fecundity species according to its size, fecundity in nature varying from 2.2 to 7.7 eggs per adult female. Rates of population increase evaluated for some sequences of parthenogenetic growing periods, reached up to 0.9. Most of the daily P/B evaluated by the cumulative growth method reached values above 1.0 (28 to 31 °C), minima being about 0.7 for lower temperatures (approximatively 26 °C). Populations were also characterized by low juvenile to adult ratios (down to 0.9) and high daily birth rates (up to 1.2). Overall, M. micrura is a highly productive, opportunistic species, well adapted to the low salinities that occur during part of the year in the ponds. Biological and population characteristics of this species, and literature data on regulation mechanisms, possible use for aquaculture, and on size in tropical species are discussed.
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  • 47
    ISSN: 1573-5095
    Keywords: bud dormancy ; growth ; morphology ; root collar diameter ; seedling quality ; survival ; sylem pressure potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study examined the relationship between root collar diameter, plant moisture stress and budbreak in three morphological grades of bareroot 1+0 slash pine (Pinus elliottii Engelm.) nursery seedlings and assessed the role of these parameters in predicting field performance potential under operational conditions. Two months after outplanting seedlings with small (≤3.2 mm) diameters exhibited greater signs of moisture stress than those with the largest (≥4.7 mm) diameters, as determined by lower xylem pressure potential values. Intermediate and large-sized seedlings (diameter 〉3.2 mm) showed earlier budbreak than smaller seedlings with more rapid shoot elongation after planting and had significantly greater survival rates for two years after planting on both a moist flatwoods and a dry sandhill planting site. However, after two years on the more favorable moist site, height and diameter measurements of seedlings with significantly smaller diameters initially did not differ from those of intermediate sized seedlings (diameter 〉3.2 and 〈4.7 mm). Large seedlings had greater second-year leader and diameter increments and attained greater total height and diameter after two years on both sites. Decreasing the proportion of smaller seedlings included in the field performance analyses increased overall mean plantation survival while increasing the proportion of large seedlings increased mean two-year total height and diameter as well as annual growth increments.
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  • 48
    ISSN: 1573-5117
    Keywords: roach (Rutilus rutilus (L.)) ; diet ; growth ; biomanipulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous studies have suggested that the roach Rutilus rutilus (L.) stock of Lake Vesijärvi is one of the main factors delaying the recovery of the lake after sewage diversion. This study is concerned with the documentation of the diet of roach in the lake. In total, 531 roach were examined. Both in the pelagial and in the littoral the roach had mixed diets in May and in September—October. The importance of zooplankton decreased and the importance of benthos and plants increased with increasing size of roach. In July, in the pelagic zone all sizes of roach fed exclusively on zooplankton (Bosmina spp.), while in the littoral zooplankton had the highest volume proportions only in the smallest (〈130 mm) roach. The frequent use of plant food and slow growth rate of large roach indicate a low availability of animal prey. As the fish densities decrease due to the mass removal taking place in the lake, the percentage of plant food in the diets of roach will probably decrease and the growth of roach will increase. Additionally, the tendency of the roach to migrate into the pelagic zone in early summer may be reduced, which would decrease their predation on the zooplankton.
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  • 49
    ISSN: 1573-5168
    Keywords: triploidy ; growth ; hormones ; growth hormone ; thyroid hormones ; gonadotropin ; gonadal steroids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Triploidy as a result of thermal shock exposure of fertilized eggs decreases the growth rate ofOreochromis aureus as compared to their diploid controls, but this is due to the higher female ratio present in triploids (86%) and the lower growth rate of females. When females and males are considered separately, the growth rate is not significantly different in diploids and triploids. Since triploidy results in a malfunctioning steroidogenesis in females (mainly testosterone (T) and 17β-estradiol (E2)), but does not affect the growth rate, it is concluded that female gonadal steroids do not influence growth unless in pharmacological concentrations. These low levels of gonadal steroids are generally accompanied by higher levels of gonadotropin (GtH), but the difference is not always significant. Despite their lower growth rate diploid females have higher plasma concentrations of growth hormone (GH) during several months compared to the triploid females and diploid males. 3,5,3′-triiodo-L-thyronine (T3) levels, however, are comparable between diploid and triploid females (except for 1 month), but higher in diploid males in 4 of the 5 months studied. 11-ketotestosterone (11kT) is always higher in males. These results indicate that the higher growth rate of males may be related to the high circulating levels of T3 and 11kT.
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  • 50
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    Fish physiology and biochemistry 12 (1994), S. 369-380 
    ISSN: 1573-5168
    Keywords: red drum ; Sciaenops ocellatus ; thyroid hormones ; diet ; growth ; body composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four separate 8-week feeding trials were conducted to assess the effects of supplementing semipurified diets with either triiodothyronine (T3) or thyroxine (T4) at 0, 2, 10, and 50 mg/kg on growth and body composition of juvenile red drum (Sciaenops ocellatus) held in artificial brackish water (6‰) and artificial seawater (32‰). At both levels of salinity, increasing doses of T3 resulted in fish with reduced weight gain, feed efficiency, condition factor (weight × 100/length3), and muscle ratio (muscle weight × 100/body weight), as well as a lighter body color. Significant (p 〈 0.05) effects of T3 on the proximate composition of whole body, liver, and muscle were variable, generally reflecting decreased lipid and protein storage in liver and muscle, respectively. The two highest doses of T3 given to seawater adapted fish increased survival. Dietary T4 supplementation had no distinctive effects on appearance, growth or proximate body composition. These results indicate that whereas T3 may function to regulate protein and lipid metabolism in red drum, dietary supplementation with T3 leads to a hyperthyroidism-induced catabolic state. The elevated endogenous thyroid hormone levels found in fish fed optimal diets may thus adequately supply tissue needs during juvenile growth.
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  • 51
    ISSN: 1573-5168
    Keywords: turbot ; growth ; linoleate ; linolenate ; lipids ; fatty acids ; prostaglandins
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    Topics: Biology
    Notes: Abstracts Three practical-type diets utilizing fishmeal and casein as the protein sources and containing fish oil (FO), safflower oil (SO) or linseed oil (LO) were fed to duplicate groups of juvenile turbot (Scophthalmus maximus) of initial weight 1.2 g for a period of 12 weeks. No differences in final weight, mortality or development of pathological lesions were evident either between duplicate tanks or between dietary treatments over this period. Fish fed diets containing SO and LO contained significantly greater amounts of liver triacylglycerol compared to fish fed FO. The major C18 polyunsaturated fatty acids (PUFA) in SO and LO diets, 18:2(n-6) and 18:3(n-3) respectively, were readily incorporated into both total lipid and individual phospholipids of turbot tissues. There was no accumulation of the Δ6-desaturation products of these fatty acids, namely 18:3(n-6) and 18:4(n-3), in any of the tissues examined. The products of elongation of 18:2(n-6) and and 18:3(n-3), 20:2(n-6) and 20:3(n-3) respectively, accumulated in both total lipid and phospholipids with the highest levels of 20:2(n-6) in liver PC and 20:3(n-3) in liver PE. Eicosapentaenoic acid [EPA, 20:5(n-3)] levels exceeded those of arachidonic acid [AA, 20:4(n-6)] in phosphatidylinositol (PI) from liver and gill of fish fed LO. EPA levels in liver PI from fish fed LO were 3-fold and 2-fold greater than SO-fed and FO-fed fish, respectively. Fish fed diets containing SO and LO had significantly reduced levels of AA in liver and muscle total lipid and lower AA in individual phospholipid classes of liver and gill compared to FO-fed fish. The concentration of thromboxane B2 was significantly reduced in plasma and isolated gill cells stimulated with calcium ionophore A23187 of fish fed SO and LO compared to those fed FO. Prostaglandin E produced by isolated gill cells stimulated with A23187 was significantly reduced in fish fed both SO and LO compared to fish fed FO.
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  • 52
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    Journal of bioenergetics and biomembranes 26 (1994), S. 137-142 
    ISSN: 1573-6881
    Keywords: Adriamycin ; ascorbate ; electron transport ; growth ; plasma membrane (HL-60 cells)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Adriamycin, 10−8–10−5 M, inhibited transplasma membrane electron transport of uninduced HL-60 cells susceptible to adriamycin and not in uninduced HL-60 cells resistant to adriamycin as measured by reduction of external ascorbate free radical. Electron flow across the plasma membrane was measured with the intact living cells by means of a simple assay procedure whereby the transported electrons were captured by ascorbate free radical to slow the rate of chemical oxidation of ascorbate. The response to adriamycin was rapid with maximum inhibition in less than 1 min. Preincubation was not required and the inhibition presumably was not mediated through effects on DNA replication or transcription. Except at the highest concentration tested of 10 µM, both transplasma membrane electron transport and growth were unaffected by adriamycin with a line of HeLa cells resistant to the drug. The findings provide evidence, using a physiological acceptor, ascorbate free radical, for a direct inhibition of transmembrane electron transport of HL-60 cells by adriamycin that correlates closely with adriamycin inhibition of cell growth. The lack of response with resistant cells suggests an alternative mechanism for adriamycin resistance not necessarily based on transport control.
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  • 53
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    Open economies review 5 (1994), S. 65-88 
    ISSN: 1573-708X
    Keywords: growth ; protectionism ; dualism ; collective action ; developing countries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This paper examines how economic growth can affect various political actors and influence trade and labor policies in a developing economy. The paper extends the Findlay-Wellisz (1982) model of endogenous trade policy to include the endogenous determination of an urban-rural wage differential along lines suggestive of the Harris-Todaro (1970) model. Under assumptions normally associated with developing economies, the model shows that growth, stimulated primarily by capital formation, can lead to the rise of protectionism and urban unrest.
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  • 54
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    Journal of bioenergetics and biomembranes 26 (1994), S. 421-433 
    ISSN: 1573-6881
    Keywords: NADH oxidase ; plasma membrane ; growth factors ; growth ; thiol/disulfide interchange ; membranes ; brefeldin A ; Golgi apparatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract An NADH oxidase activity of animal and plant plasma membrane is described that is stimulated by hormones and growth factors. In plasma membranes of cancer cells and tissues, the activity appears to be constitutively activated and no longer hormone responsive. With drugs that inhibit the activity, cells are unable to grow although growth inhibition may be more related to a failure of the cells to enlarge than to a direct inhibition of mitosis. The hormone-stimulated activity in plasma membranes of plants and the constitutively activated NADH oxidase in tumor cell plasma membranes is inhibited by thiol reagents whereas the basal activity is not. These findings point to a thiol involvement in the action of the activated form of the oxidase. NADH oxidase oxidation by Golgi apparatus of rat liver is inhibited by brefeldin A plus GDP. Brefeldin A is a macrolide antibiotic inhibitor of membrane trafficking. A model is presented where the NADH oxidase functions as a thiol-disulfide oxidoreductase activity involved in the formation and breakage of disulfide bonds. The thiol-disulfide interchange is postulated as being associated with physical membrane displacement as encountered in cell enlargement or in vesicle budding. The model, although speculative, does provide a basis for further experimentation to probe a potential function for this enzyme system which, under certain conditions, exhibits a hormone- and growth factor-stimulated oxidation of NADH.
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  • 55
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    Journal of chemical ecology 20 (1994), S. 2743-2749 
    ISSN: 1573-1561
    Keywords: Hymenoptera ; Symphyta ; Tenthredinidae ; predation ; chemical defense ; ants ; Formicidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Evidence is presented from predation tests with ants (Formica exsectoides) that the slimy coating ofCaliroa cerasi and the waxy investiture ofEriocampa ovata serve in defense.
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  • 56
    ISSN: 1573-1561
    Keywords: Hymenoptera ; Alloxystidae ; hyperparasitoids ; ants ; Formicidae ; interactions ; aggression ; chemical defense ; 6-methyl-5-hepten-2-one ; actinidin ; biological significance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Foraging females of the aphid hyperparasitoidAlloxysta brevis were attacked by honeydew-collecting workers of the antLasius niger at the first encounter. However, ants abandoned their attacks quickly, and foragingA. brevis remained unmolested for a subsequent time interval of approximately 5 min, which is long enough for the hyperparasitoid to oviposit successfully. Furthermore, freshly killed intactA. brevis were disregarded by ants, while decapitated specimens were readily removed. We present evidence thatA. brevis females release a mandibular gland secretion, which contains 6-methyl-5-hepten-2-one, actinidin, and unidentified iridoids, in response to an ant attack. This secretion functions both as a measure of self-defense if the female is seized by an ant worker and as a repellent, which prevents ant attacks during subsequent encounters. This is the first evidence for chemical defense in a hymenopterous parasitoid. It enablesA. brevis females to hyperparasitize ant-attended aphids that constitute a major proportion of their hosts and significantly reduces mortality by ectohyperparasitoids.
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  • 57
    ISSN: 1573-1561
    Keywords: Chemical communication ; nestmate recognition ; role of the queen ; colony closure ; aggressive behavior ; pheromone ; cuticular hydrocarbons ; ant ; Messor barbarus ; Hymenoptera ; Formicidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The results of laboratory experiments carried out with both monogynous and artificially polygynousMessor barbarus ant colonies (which under natural conditions are always monogynous) have shown that the workers belonging to monogynous colonies were able to discriminate between intruders from other monogynous colonies and those from polygynous (di- and trigynous) ones. What mechanisms are involved in this discriminatory ability? Since differences in the relative proportions of the hydrocarbons they carry are known to convey complex messages that are used for recognition purposes, it was proposed here to investigate whether there existed any differences in the proportions of the various hydrocarbons carried by the diverse categories of intruders tested in our experiments. It emerged that one set of hydrocarbons, which were usually present in rather small proportions and included all the families that constitute this species' chemical signature (n-alkanes, mono-, di-, and trimethylalkanes), was characteristically associated with workers from monogynous colonies. Another set of hydrocarbons, which included some of the above components, mostly in larger relative proportions, can be said to have characterized the digynous and trigynous colonies.
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  • 58
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    Journal of chemical ecology 20 (1994), S. 785-802 
    ISSN: 1573-1561
    Keywords: Hymenoptera ; Agaonidae ; evolution ; fig wasps ; host finding ; volatile attractants ; Ficus ; Moraceae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Fig wasps (Chalcidoidea; Agaonidae) are intimately associated with the 750 or so species of fig trees (Ficus, Moraceae). Each tree species is usually pollinated by a single species of wasp belonging to the subfamily Agaoninae, while other wasps of the family are parasitoids or seed predators. Previous experiments have shown that the wasps are attracted to the trees by volatiles emanating from the figs. Using fig-bearing trees and arrays of sticky traps baited with figs, we investigated the specificity of wasp attraction and its timing. The pollinators of two closely relatedFicus species were specifically attracted to figs of their host species and only at the time when figs were ready to be pollinated. Some nonpollinating fig wasps appear to respond to the same volatile cues.
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  • 59
    ISSN: 1573-1561
    Keywords: Amblyomma variegatum ; Acarina ; Ixodidae ; dermal glands ; Solenopsis geminata ; Hymenoptera ; Formicidae ; defensive secretion ; entomopathogenic bacteria ; antibiotic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract When they are mechanically disturbed, all instars of the tropical bont tickAmblyomma variegatum exude droplets of a liquid on the dorsal, lateral, and ventral cuticle. These spread out and quickly evaporate. In this study, the possible role of these secretions was investigated in relation to predators and pathogens. In laboratory bioassays, it was demonstrated that the secretions from engorged larvae, nymphs, and females have an antibiotic activity against the bacteria speciesBacillus thuringiensis andSerratia marcescens, combined with a repellent effect on a potential predator, the fire-antSolenopsis geminata.
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  • 60
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Pieridae ; Hymenoptera ; Braconidae ; cabbage ; Brussels sprouts ; behavior ; tritrophic interactions ; green-leaf volatiles ; herbivore-induced synomones ; elicitor ; caterpillar regurgitant ; Brassica oleracea ; Pieris brassicae ; Cotesia glomerata ; parasitoid ; wasp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Induction of plant defense in response to herbivory includes the emission of synomones that attract the natural enemies of herbivores. We investigated whether mechanical damage to Brussels sprouts leaves (Brassica oleracea var.gemmifera) is sufficient to obtain attraction of the parasitoidCotesia glomerata or whether feeding byPieris brassicae caterpillars elicits the release of synomones not produced by mechanically damaged leaves. The response of the parasitoidCotesia glomerata to different types of simulated herbivory was observed. Flight-chamber dual-choice tests showed that mechanically damaged cabbage leaves were less attractive than herbivore-damaged leaves and mechanically damaged leaves treated with larval regurgitant. Chemical analysis of the headspace of undamaged, artificially damaged, caterpillar-infested, and caterpillar regurgitant-treated leaves showed that the plant responds to damage with an increased release of volatiles. Greenleaf volatiles and several terpenoids are the major components of cabbage leaf headspace. Terpenoids are emitted in analogous amounts in all treatments, including undamaged leaves. On the other hand, if the plant is infested by caterpillars or if caterpillar regurgitant is applied to damaged leaves, the emission of green-leaf volatiles is highly enhanced. Our data are in contrast with the induction of more specific synomones in other plant species, such as Lima bean and corn.
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  • 61
    ISSN: 1573-1561
    Keywords: Hymenoptera ; Formicidae ; army ants ; Dorylinae ; Aenictus ; Dorylus ; Eciton ; Ecitoninae ; Dufour gland ; mandibular gland ; postpygidial gland ; ant secretions ; chemotaxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The Dufour glands ofAenictus rotundatus contain a complex mixture of terpenoids with geranylgeraniol comprising over 50% of the secretion. Some novel compounds have been tentatively identified as higher homologs of 1,3,3-trimethyl-2,7-dioxabicyclo[2,2,1]heptane based on GC-MS data. The Dufour gland secretion ofA. rotundatus is more similar in composition to the secretions of members of the subfamily Ecitoninae than to its closer relatives from the tribe Dorylini, a result that further complicates studies on the phylogeny of army ants. The mandibular glands ofA. rotundatus contain a mixture of 4-methyl-3-heptanone and limonene in trace amounts, and the well-developed postpygidial glands contain methyl anthranilate only.
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  • 62
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    Journal of chemical ecology 20 (1994), S. 1053-1056 
    ISSN: 1573-1561
    Keywords: Honey bee ; Apis mellifera ; Hymenoptera ; Apidae ; pheromone ; attractant ; Nasonov ; swarms ; citral ; geraniol ; nerolic acid ; geranic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A crossover experimental design was established to test the attractancy of Nasonov pheromone to reproductive swarms of honey bees. Nineteen swarms were attracted to artificial nest cavities containing a slow-release blend of the Nasonov components citral, geraniol, and nerolic + geranic acids, and only four swarms were attracted to pheromone-free artificial nests. The results indicate that Nasonov pheromone plays a key role in the attraction of honey bee swarms to nest cavities.
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  • 63
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    Journal of chemical ecology 20 (1994), S. 1113-1119 
    ISSN: 1573-1561
    Keywords: Hymenoptera ; Formicidae ; Reptilia ; Amphisbaenidae ; Blanus cinereus ; prey odor ; harmful prey ; tongue-flicking
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We tested the ability of amphisbaenians (Blanus cinereus) to discriminate between odors of ant species selected as prey (Pheidole pallidula) and odors of potentially harmful ant species (Messor barbarus) that are avoided. Tongue-flick rate to swabs impregnated with ant odors, cologne, or deionized water differed among treatments, showing that amphisbaenians were able to discriminate ant species odors. Amphisbaenians showed an aggressive response and bit applicators bearing the odor of harmful ants, while the odor of prey ants did not elicit bites to swabs. The possible evolutionary advantage of identifying and avoiding harmful ants is discussed in relation to the fossoriality of amphisbaenians.
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  • 64
    ISSN: 1573-1561
    Keywords: Polistes fuscatus ; Hymenoptera ; Vespidae ; cuticular hydrocarbons ; discriminant function analysis ; nestmate recognition ; social wasps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The cuticular chemicals of 124 individual wasps (foundresses and workers) from 23 colonies ofPolistes fuscatus were analyzed. The compounds identified, all of which were hydrocarbons, were similar to those of other vespid wasps in that the bulk of the hydrocarbons were 23–33 carbons in chain length. However, the hydrocarbon profile ofP. fuscatus differed from those of its congeners in its proportions of straight-chain alkanes, methylalkanes, and alkenes. Three of the 20 identified hydrocarbons, 13- and 15-MeC31, 11,15- and 13,17-diMeC31, and 13-, 15-, and 17-MeC33, had properties postulated for recognition pheromones: colony specificity, efficacy in assigning wasps to the appropriate colony, heritability, lack of differences between foundresses and workers, and distinctive stereochemistry.
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  • 65
    ISSN: 1573-1561
    Keywords: Trail pheromone ; Hymenoptera ; Formicidae ; methyl 4-methylpyrrole-2-carboxylate ; poison gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The trail pheromone ofAcromyrmex subterraneus subterraneus consists simply of the heterocyclic ester methyl 4-methylpyrrole-2-carboxylate in the venom reservoir of workers at about 1.2 ng/ant. No pyrazines were detected, and no enhancement of trail-following was observed when pyrazines were added to the pyrrole compound.
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  • 66
    ISSN: 1573-1561
    Keywords: Cotesia rubecula ; Hymenoptera ; Braconidae ; Lepidoptera ; Pieridae ; Plutellidae ; Pieris rapae ; Brassica oleracea ; Plutella xylostella ; Phaselus vulgaris ; Geranium molle ; tritrophic interactions ; infochemicals ; volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The role of airborne infochemicals in host selection by the parasitoidCotesia rubecula (Marshal) (Hymenoptera: Braconidae) was examined in a wind tunnel. To elucidate the role of volatile chemicals in attractingC. rubecula to cabbage infested by the host [Pieris rapae L. (Lepidoptera: Pieridae)], the potential sources of volatiles related toP. rapae infestation on cabbage were tested individually. The responses of females to nonhost plant species, bean and geranium, as well as to frass of a nonhost lepidopteran were also examined.C. rubecula was attracted to cabbage previously infested byP. rapae and to frass and regurgitate ofP. rapae. No attraction was observed to larvae ofP. rapae alone. Females were also attracted to mechanically damaged cabbage, cabbage previously infested byPlutella xylostella L. (Lepidoptera: Plutellidae) (a nonhost lepidopteran herbivore), and cabbage previously infested by snails (a nonhost, noninsect herbivore). Intact cabbage, bean, and geranium plants elicited no attraction. A low frequency of attraction was observed to mechanically damaged bean and geranium. Attraction was also observed to frass ofP. xylostella. Volatiles from cabbage related to damage, and volatiles from frass and regurgitate of the host seem to play an important role in guidingC. rubecula to plants infested by its host.
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  • 67
    ISSN: 1573-1561
    Keywords: Cotesia rubecula ; Hymenoptera ; Braconidae ; Lepidoptera ; Pieridae ; Plutellidae ; Pieris rapae ; Plutella xylostella ; Helix aspera ; Brassica oleracea ; Phaseolus vulgaris ; tritrophic interactions ; synomones ; infochemicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The responses of the parasitoidCotesia rubecula to differently damaged cabbages were recorded during a series of choice tests. To determine if flyingC. rubecula can discriminate differences in the blend of volatiles emitted by cabbages damaged by different causes and how plant volatiles released from a distant source affect the searching behavior ofC. rubecula once searching on a plant, wasps were presented with a choice of plants located one behind the other and separated by a distance of 15 cm. The sources of damage were: cabbage damaged by the host (Pieris rapae), by a nonhost lepidopteran herbivore (Plutella xylostella), by a nonhost, noninsect herbivore (snail), and by mechanical means. The results showed that the site of first landing and the time spent searching on the leaves was influenced by the type of damage inflicted on plants. Wasps preferred to land on cabbages damaged by host and nonhost species of Lepidoptera over those damaged by snails and mechanical means. No preference was observed for first landing between cabbages damaged by the two species of Lepidoptera or between cabbages damaged by snails and mechanical means. Cabbage damaged byP. rapae was searched most intensively, followed by cabbage damaged byP. xylostella, cabbage damaged by snails, and cabbage damaged by mechanical means.C. rubecula differentiates between the volatile blends emitted by differently damaged cabbages, and it is attracted to volatiles related to recent lepidopteran damage. Wasps searched longer on freshly damaged than on leaves with older damage.
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  • 68
    ISSN: 1573-1561
    Keywords: Galleria mellonella ; Apis mellifera ; Pyralidae ; Apidae ; Lepidoptera ; Hymenoptera ; greater wax moth ; honeybee ; propolis ; plant resins ; phenolics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Bee propolis is a sticky amalgamation of plant resins collected by honeybees (Apis mellifera L.) and used in the hive for filling cracks and repairing combs. Propolis contains a diversity of compounds of plant origin, and is reported to have medicinal, antimicrobial, insecticidal, and phytotoxic properties. We examined the physical and chemical composition of North American samples of bee propolis from several sites in North America and tested for bioactivity against larvae of the greater wax moth (Galleria mellonella L.), a common apiary pest. The amount of methanol-extractable resin in samples from Ohio and Georgia ranged from 24% to 79% by weight. Propolis collected from hives in Ohio was more chemically diverse (over 30 compounds detected by paper chromatography) than material from south Georgia (fewer than 10 major compounds) and contained a lower proportion of methanol-insoluble beeswax. The paper chromatographic surveys revealed little variation in the chemical profile of specific hives over a six-month period and no differences between propolis from adjacent hives. Four flavonoids were identified from propolis collected in Ohio: kaempferol, galangin, 3,3′-dimethoxyquercetin and 3-methoxykaempferol. When mixed into artificial diet, fractionated propolis reduced larval growth of the greater wax moth, but not dramatically. An array of phenolics reported from propolis (caffeic acid, chrysin, ferulic acid, galangin, kaempferol, and quercetin) were bioassayed individually for effects on larvae, but none reduced larval growth at the concentrations tested, suggesting that wax moths are tolerant of some phenolics in their diet.
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  • 69
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    Journal of chemical ecology 20 (1994), S. 1583-1594 
    ISSN: 1573-1561
    Keywords: Ovipositional stimulants ; Catolaccus grandis ; Hymenoptera ; Pteromalidae ; short-chain hydrocarbons ; artificial diet ; kairomones ; synomones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Oviposition behavior was elicited fromCatolaccus grandis (Burks) (Hymenoptera: Pteromalidae) females, an ectoparasitoid of the boll weevil,Anthonomus grandis Boheman (Coleoptera: Curculionidae), by smears of freshly cut cotton bolls or smears of extracts prepared with boll weevil damaged or undamaged cotton boll tissues. Oviposition behavior was also elicited fromC. grandis females by smears made withn-pentane,n-hexane,n-heptane, and isooctane. This is the first report of oviposition behavior elicited for any parasitoid by these short-chain saturated hydrocarbons (alkanes), introducing a new concept on the chemical mediation of parasitoid behavior during host selection. Oviposition behavior was also elicited fromC. grandis females by volatiles emanating from an artificial diet devoid of insect components that was specifically developed for the in vitro rearing of ectoparasitoids. The possible use of a synergistic combination ofn-hexane and diet to optimize the mechanized production of noncontaminated eggs is also discussed.
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  • 70
    ISSN: 1573-1561
    Keywords: Pieris rapae ; Lepidoptera ; Pieridae ; Plutellidae ; Plutella xylostella ; Cotesia rubecula ; Hymenoptera ; Braconidae ; Brassica oleracea capitata ; infochemicals ; plant volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract To elucidate the identity of the volatile compounds that could be involved in the searching behavior of the parasitoidCotesia rubecula Marshall (Hymenoptera: Braconidae), the volatiles released by cabbage and frass of Lepidoptera feeding on cabbage were collected and analyzed using a gas chromatograph-mass spectrometer. The volatiles emitted by intact cabbage were α-pinene, β-pinene, myrcene, 1,8-cineole,n-hexyl acetate,cis-3-hexen-1-yl acetate, and dimethyl trisulfide. Mechanical damage on an intact plant induced the release of two more compounds,trans-2-hexenal and 1-methoxy-3-methylene-2-pentanone. Current feeding by larvae ofPieris rapae L. (Pieridae) induced the plant to release all the compounds released after mechanical damage and additionally 4-methyl-3-pentenal and allyl isothiocyanate. Current feeding by larvae ofPlutella xylostella L. (Plutellidae) induced the plant to release all the compounds present after mechanical damage and additionally allyl isothiocyanate. The volatiles emitted after feeding by the lepidopterans had ceased were the same as those emitted by cabbage damaged by mechanical means. The blend of volatiles emitted by frass was comprised of plant chemicals, mainly sulfur compounds. Frass ofP. rapae emitted allyl isothiocyanate, methyl isothiocyanate, methyl propyl sulfide, dimethyl trisulfide,S-methyl methane thiosulfinate, 4-methyl-3-pentenal,trans-2-hexenal, and 2,3-dihydro-4-methyl furan. Frass ofP. xylostella emitted only dimethyl trisulfide andS-methyl methane thiosulfinate. The blend of volatiles emitted by frass is herbivore-species specific.
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  • 71
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    Nutrient cycling in agroecosystems 40 (1994), S. 235-242 
    ISSN: 1573-0867
    Keywords: fertilization ; growth ; natural survival ; root pruning ; Pterocarpus angolensis ; symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Observations on growth behaviour of naturally regenerated and irrigated nursery seedlings ofPterocarpus angolensis were made in Morogoro, Tanzania. Following their natural survival strategy, seedlings build up a robust taproot. The above-ground shoot produced in a year dies back in the field during the dry period whereas the taproot expands during the rainy seasons. In the nursery, the type of symbiosis formed by irrigated seedlings was assessed and trials of taproot pruning and fertilization were carried out. Seedlings formed VA mycorrhizae and nodules. Root pruning sharply depressed seedling growth. Fertilization with nitrogen and phosphorus disturbed the shoot/root ratio in favour of the shoots and inhibited nodulation. We concluded that manipulation of the root and the root environment ran contrary to the seedling's own, natural survival strategy.
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  • 72
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    Agroforestry systems 26 (1994), S. 171-184 
    ISSN: 1572-9680
    Keywords: windbreaks ; Sahel ; Niger ; mortality rate ; growth ; establishment ; nutritive value ; calorific value
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tree and windbreak species considered for the Southern Sahelian Zone (SSZ) of West Africa have to be evaluated following multiple criteria such as fast establishment, shelter efficiency, production of feed for livestock, and firewood. An on-station experiment was conducted on a sandy soil in southwest Niger between 1988 and 1993 to assess the establishment, growth, nutritive, and calorific values of seven species considered for windbreaks:Andropogon gayanus, Bauhinia rufescens, Acacia holosericea, Acacia nilotica, Acacia senegal, Faidherbia albida, andAzadirachta indica. Mortality and stem diameter were monitored twice a year. From 20 months after planting (MAP) onwards, species were annually trimmed to 2 m height and to 1 m sidewards of the main stem. Fresh and dry weight of leaves, twigs, wood, and fruits were recorded. Leaves were analyzed for crude protein (CP) and metabolizable energy (ME) content. Wood was analyzed for its gross calorific value and its ash content. At 56 MAP, all species had less than 5% morality, except forA. holosericea, which had a mortality rate of 15.9%.A. indica andA. holosericea showed the highest stem diameter among species with 12.2 and 11.8 cm, respectively, at 56 MAP.A. senegal had the highest dry matter production with 22.3 t ha−1 at 56 MAP, followed byA. indica (12.0 t ha−1),A. holosericea (11.7 t ha−1) andB. rufescens (11.2 t ha−1), butA. holosericea was most productive at earlier harvests. OnlyA. senegal (6.4 t ha−1) andA. indica (5.1 t ha−1) had a significant wood production, whereasA. holosericea (5.7 t ha−1) had the highest phyllodes production. Leaves ofA. senegal andA. indica had highest CP contents of 258 and 214 g kg−1, respectively. The leaves of all species exceptA. gayanus had a higher CP:ME ratio than natural pasture in the region. The calorific values of firewood did not differ significantly among the species.A. nilotica, the species with one of the lowest firewood production, had the highest Fuelwood Value Index of 6.6. The choice of species for planting trees and windbreaks in the SSZ must be oriented along these criteria. Further research should be directed towards cost-benefit analyses, land tenure and property rights in combination with surveys on local knowledge of rural people.
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  • 73
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    Hydrobiologia 281 (1994), S. 115-122 
    ISSN: 1573-5117
    Keywords: Trisopterus luscus ; growth ; age ; bib ; Atlantic ; Asturian coast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The age and growth of an Asturian population of the ‘bib’ Trisopterus luscus L. were studied based on 990 bibs from monthly catches from October 1986 until October 1988. Using the estimates of age taken from otolith readings, it was possible to construct a growth curve for the whole range of ages and demonstrate that most growth takes place during the first two years of life. The maximum age was found to be 5 years. The largest fish caught during the investigation measured 430 mm in standard length. The different growth rates of males and females show that in most cases the mean standard length of females was equal to or greater than that of the males.
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  • 74
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    Hydrobiologia 291 (1994), S. 125-130 
    ISSN: 1573-5117
    Keywords: Biomphalaria ; intermittent starvation ; survival ; growth ; reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mature Biomphalaria glabrata, submitted to four weeks of varied starvation times (0, 1, 3, 5, 6 & 7 d.week−1, were thereafter refed during four weeks. The different intermittent starvation times had no significant effect on snails survival. As weekly starvation increased, the rate of change in body weight and fecundity decreased. In snails fed one or two d.week−1, the rate of change in body weight was negative, while fecundity remained at a low level. Continuous hunger stopped oviposition. Starvation had no further effect on body growth after the first week of refeeding; however, its effect on fecundity remained significant over the two first weeks.
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  • 75
    ISSN: 1573-1561
    Keywords: Floral oils ; cacao ; Theobroma cacao ; Sterculiaceae ; attraction ; Diptera ; Ceratopogonidae ; Cecidomyiidae ; Hymenoptera ; Apidae ; pollination ; 1-pentadecene ; n-pentadecene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Steam-distilled floral fragrance oils from nine distinctive cultivars ofTheobroma cacao L. (Sterculiaceae) in Costa Rica were examined with GC-MS to determine whether or not major differences existed among these cultivars for volatile constituents comprising 50% or more of the samples. The cultivars selected for floral oil analyses were chosen to represent diverse cultivars having supposedly different genetic backgrounds and histories of artificial selection for agronomic purposes. Cluster analysis revealed two major groupings of cultivars: those with higher molecular weight dominant compounds, and those having lower molecular weight compounds. Additionally, one cultivar, Rim-100, selected from criollo or ancestral-type cacao in Mexico and resembling criollo in the appearance of flowers and fruits, formed an extreme group having the highest molecular weight profile for major volatile compounds. Based upon these analyses, bioassays using McPhail traps were performed in an abandoned cacao plantation in northeastern Costa Rica during rainy and dry seasons to determine the relative attraction of these oils to flying insects. Bioassays revealed that the Rim-100 cultivar attracted by far the greatest numbers of cacao-associated midges (Diptera: Ceratopogonidae and Cecidomyiidae), as well as stingless bees (Hymenoptera: Apidae: Meliponinae), suggesting that a floral fragrance having high-molecular-weight volatiles is more potent as an attractant to flying insects than floral oils having lower-molecular-weight compounds. It is suggested that Rim-100 more closely resembles an ancestral or wild-type cacao than the other cultivars examined, and therefore it is more effective in attracting opportunistic dipteran floral visitors and pollinators than other cultivars in plantation settings. Several of the major volatile compounds found in the floral oils ofT. cacao and other species ofTheobroma occur in mandibular and other exocrine glands in various bees, including stingless bees and halictids, known visitors ofTheobroma flowers. These compounds are particularly present in noncultivated species ofTheobroma and have much more noticeable fragrances than the seemingly scentless flowers of cultivatedT. cacao selected for agriculture. It is hypothesized that the floral attraction system of ancestral or wild (noncultivated)T. cacao and other species ofTheobroma may have evolved to attract certain bees as their principal pollinators in natural habitats in the Neotropics, with a floral reward hypothesized as being sociochemicals needed by bees for mating, foraging, territorial defense, etc. Because of the many generations of extensive selection by cloning for desired cultivars,T. cacao might have lost much of its original floral attraction system for bees, instead being pollinated opportunistically by dipterans in plantation habitats. This may help to explain why natural pollination in cultivatedT. cacao is generally very poor relative to observed levels of fruit-set in wild or noncultivated species ofTheobroma.
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  • 76
    ISSN: 1573-1561
    Keywords: Honey bee ; Apis mellifera ; Hymenoptera ; Apidae ; gas chromatography ; electroantennogram ; conditioned proboscis extension ; olfactory discrimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A novel technique for the simultaneous monitoring of electroan-tennogram (EAG) and conditioned proboscis extension (CPE) responses of honey bees to the effluent from a gas chromatograph (GC) was developed to locate biologically active components in blends of plant volatiles and to investigate odor recognition at the peripheral and behavioral levels. A six-component mixture, comprising compounds previously identified as oilseed rape floral volatiles, was used as the stimulus. Standard CPE and EAG recordings were done as a reference. EAG responses were elicited from unconditioned bees by all the components presented either in the coupled or the standard mode. Conditioned bees gave larger EAG responses than unconditioned bees, suggesting that antennal sensitivity is enhanced by conditioning. At the behavioral level, in both the standard and the coupled modes, only conditioned bees showed the proboscis extension response, with the majority of individuals responding to linalool, 2-phenylethanol, and benzyl alcohol.
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  • 77
    ISSN: 1573-1561
    Keywords: Veratrum album ; ceveratrum alkaloids ; plant-insect interactions ; sequestration ; hemolymph ; feeding deterrence ; Rhadinoceraea nodicornis ; Aglaostigma sp. ; Hymenoptera ; Tenthredinidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The larvae of the specialist sawflyRhadinoceraea nodicornis Konow (Hymenoptera, Tenthredinidae) store in their hemolymph ceveratrum alkaloids originating from the host plantVeratrum album L. (Liliales, Melanthiaceae). The major alkaloid found in the hemolymph is 3-acetyl-zygadenine. Qualitative and quantitative data showed that the plant alkaloid 3-angeloylzygadenine is most probably metabolized in the larval gut to zygadenine and then acetylated. A still unidentified alkaloid with a molecular weight of 591 Da was detected in plant leaves as well as in the gut, hemolymph, and excrement of larvae. Protoveratrine A and B, on the other hand, seem to be degraded by the larvae. These findings indicate that the pathway of ceveratrum alkaloids inR. nodicornis larvae is fourfold: direct sequestration, metabolism followed by sequestration, excretion of intact alkaloids, and degradation. In contrast, no ceveratrum alkaloids were detected in the hemolymph and excrement of larvae of the generalist sawflyAglaostigma sp. fed withV. album leaves. Bioassays with the antMyrmica rubra L. proved that the hemolymph ofR. nodicornis larvae is highly deterrent and toxic. In bioassays evaluating defensive efficiency against predators (ants, spiders, and bushcrickets), no larvae were eaten. Ceveratrum alkaloids were also detected in the hibernating prepupae ofR. nodicornis. In feeding bioassays, the shrewCrocidura russula Hermann rarely fed upon prepupae, suggesting that this stage is also protected from predation to some degree. In field surveys, the only parasitoids recorded were two ichneumonid species that are believed to be specialized onR. nodicornis. Bioassays and field observations enable us to suppose thatR. nodicornis and its enemies produce a food web of ion connectance.
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    World journal of microbiology and biotechnology 10 (1994), S. 505-509 
    ISSN: 1573-0972
    Keywords: Acetic acid ; chemostat ; Geotrichum ingens ; growth ; inhibition ; kinetics ; monocarboxylic acids ; propionic acid ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Growth of Geotrichum ingens in batch cultures was completely inhibited by 47 g acetic acid/l or 33 g propionic acid/I. With mixtures of acetic and propionic acids, however, growth only ceased at 55 g/l. Acetic acid inhibited growth linearly, whereas propionic acid inhibited growth non-linearly. In continuous culture, two steady states at each dilution rate were observed at high dilution rates for acetic acid and propionic acid. The highest yield coefficient (0.69 g cells/g substrate) was achieved with propionic acid as substrate. On both substrates and their mixtures, the protein content of the biomass increased when the dilution rate was increased.
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  • 79
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    Evolutionary ecology 8 (1994), S. 315-327 
    ISSN: 1573-8477
    Keywords: mortality ; age at maturity ; growth ; reproductive investment ; life history theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Arctic charrSalvelinus alpinus life histories are very variable, both within and between localities. In some lakes we find more than one phenotype (in Lake Thingvallavatn there are four), each phenotype having a characteristic life history. In spite of this, the life histories of 44 populations of Arctic charr from throughout its range of distribution can be described accurately by a number of dimensionless indices made up from some common life-history descriptors (age and length at maturity, theoretical maximal length, instantaneous rate of natural mortality). This is contrary to what we previously have found for Brown troutSalmo trutta and we discuss this difference. We also test a new model which seeks to predict reproductive effort based on information on relative length at maturity (L α)/L inf) and age at maturity. The model did not fit the observed reproductive investment data.
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  • 80
    ISSN: 1570-7458
    Keywords: Vitis vinifera ; Empoasca vitis ; leafhopper ; photosynthesis ; transpiration ; stomatal conductance ; mesophyll conductance ; growth ; yield ; fruit quality ; starch ; carbohydrate reserves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The impact of the grape leafhopper,Empoasca vitis, on leaf gas exchange, plant growth, yield, fruit quality and carbohydrate reserves of the grapevines,Vitis vinifera L., was studied. Gas exchange was measured on the discolored (red) and the green parts of infested main leaves and on leaves from uninfested vines. Photosynthesis and mesophyll conductance were severely reduced on main leaves showing leafhopper feeding symptoms. The stomatal conductance of the red leaf section of infested main leaves was lower than on undamaged control leaves. Additionally, the red leaf section of infested main leaves showed lower transpiration rates when compared to the green parts of the same leaves and to undamaged control leaves. Gas exchange processes of lateral leaves were not affected by leafhopper feeding. Leafhopperload on main leaves was correlated to visual damage symptoms. At 71.8 leafhopper-days per leaf up to 40% of the main leaf area of the infested plants was discolored from the borders towards the center. Lateral leaves showed no feeding symptoms. Shoot diameter, pruning weight and carbohydrate reserves in the wood were not affected by leafhoppers. Lateral leaf area growth was significantly stimulated on plants infested by leafhoppers. No decrease in yield and fruit quality with leafhopper-loads up to 71.8 leafhopper-days per leaf were observed.
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    Entomologia experimentalis et applicata 69 (1993), S. 33-39 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Aphidiinae ; aphid ; sex pheromone ; parasitoid ; Praon volucre ; Aphidius rhopalosiphi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Laboratory and field experiments provided evidence for the release of sex pheromones by virgin femalePraon volucre Haliday andAphidius rhopalosiphi De Stefani-Perez (Hymenoptera: Braconidae, Aphidiinae). In Petri dish biosassays, rubber or vermiculite models treated with crude virgin female extracts were frequently approached by males and elicited rapid wing-fanning behaviour and copulation attempts. Delta-shaped water traps containing live virgin females caught large numbers of conspecific males when placed in winter wheat crops. Trapping slightly below crop height resulted in higher catches than trapping above the crop canopy.
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    Entomologia experimentalis et applicata 68 (1993), S. 1-7 
    ISSN: 1570-7458
    Keywords: Hybridoma ; detectability period ; venom ; predation ; serology ; protease ; Vespidae ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to derive quantitative estimates of predation rate from serological gut analysis data, one must have an estimate of the interval during which a meal can be detected after feeding. In practice this has turned out to be ‘Dmax,’ defined as ‘...the time from finishing a meal until that meal could just no longer be detected in any individuals.’ However Dmax substitutes an absolute limit for what is really a continuous variable with significant variation. We examined this problem in a study of the detectability ofHelicoverpa zea Boddie (Lepidoptera: Noctuidae) fifth instar remains in the guts ofPolistes metricus Say (Hymenoptera: Vespidae). Wasps were maintained onTrichoplusia ni (Hübner) (Lepidoptera: Noctuidae) fifth instars before being fed a singleH. zea fifth instar. They were killed and frozen at 0, 24, 48 and 96 h intervals, with those held for more than 24 h fed a singleT. ni fifth instar at 24 h intervals in order to simulate continued feeding. Wasp abdomens were assayed by immunodot, using a monoclonal antibody toH. zea arylphorin. There was a logarithmic decay in the proportion ofP. metricus positive over time, a singleH. zea fifth instar meal having a detectability half-life of 19.4 h at field temperatures. If prey antigen detectability decays exponentially, then a detectability half-life is a more appropriate unit of detectability than an absolute detectability period.
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    Entomologia experimentalis et applicata 68 (1993), S. 219-229 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Aphidiidae ; Homoptera ; Aphididae ; Schizaphis graminum ; wheat ; tritrophic interactions ; learning ; host-habitat location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of experience on the responsiveness of the aphidiid parasitoidLysiphlebus testaceipes (Cresson) (Hymenoptera: Aphidiidae) to host-associated cues was investigated using a wind-tunnel bioassay. Naive females were able to discriminate between uninfested wheat (Triticum aestivum L.) and wheat infested withSchizaphis gramimum (Rondani) (Homoptera: Aphididae), but oviposition experience significantly increased the parasitoid's propensity to respond to aphid-infested plants with upwind, targeted flight. The behavioural change associated with such experience was acquired rapidly (within five minutes) and persisted for at least 24 h. The parasitoid could be successfully conditioned to associate a novel odour with the presence of hosts, suggesting that the increase in response to aphid-infested plants which occurs as a result of experience is probably due to associative learning of olfactory cues from the plant-aphid complex.
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  • 84
    ISSN: 1570-7458
    Keywords: Chrysoperla carnea ; food consumption ; growth ; development ; efficiency of food utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Larvae of the common green lacewing,Chrysoperla carnea (Stephens)(Neuroptera: Chrysopidae), were fed either an optimal or a suboptimal number of eggs of the mothAnagasta kuehniella (Zeller) in the first and/or second larval instar, or in all three larval instars. Parameters for the duration, growth, and the efficiency of conversion of ingested food to body substance (ECI) of each instar were established for seven different dietary regimes. Larvae that had a suboptimal food supply in the first instar had a significantly longer developmental time, gained significantly less weight, and had a slightly lower ECI in that instar larvae. Suboptimally-fed second less, but remained only slightly longer in that instar and had a similar ECI to optimally-fed second instar larvae. The developmental time of suboptimally-fed third instar larvae was similar to that of optimally-fed larvae of that stage. Whereas the growth of the former was significantly less than that of larvae optimally fed in that instar, the ECI of the former was significantly higher. Despite the relatively smaller size of larvae fed suboptimally in the first and/or second instar, when such larvae were subsequently supplied with an overabundance of prey eggs, they consumed approximately the same number of eggs during the remainder of their larval life as did larvae whose food supply had not been restricted previously. When larvae were allowed to consume different numbers of eggs in their third instar, their gain in weight and therefore the weights attained by the resulting adults (based on the weights of 3-day-old cocoons) had a highly significant positive correlation with the number of eggs consumed in this instar.
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  • 85
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    Entomologia experimentalis et applicata 67 (1993), S. 135-142 
    ISSN: 1570-7458
    Keywords: nutrition ; utilization ; efficiency ; growth ; metabolism ; energy ; respirometry ; Pieris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conventional gravimetry and a combination of gravimetry and respirometry were compared for their precision in measuring respiration and metabolic efficiency of growth of final stadiumPieris brassicae L. (Pieridae, Lepidoptera) caterpillars. This was done both for caterpillars feeding on an artificial diet and for caterpillars feeding on excised leaf material of a host plant,Brassica oleracea L. Gravimetry produced significantly greater variation in the total amount of matter respired and the metabolic efficiency than indirect calorimetry for caterpillars feeding on plant material, while the two methods gave similar results for the caterpillars reared on a meridic artificial diet. Respirometry (indirect calorimetry) revealed that caterpillars feeding on the artificial diet were growing with a higher metabolic efficiency than caterpillars feeding on the host plant. This difference was not revealed by conventional gravimetry. It is argued that metabolic efficiencies as derived from gravimetric budget calculations are subject to a number of random errors that distort precise determination of metabolic efficiencies in studies involving plant food.
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  • 86
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    Entomologia experimentalis et applicata 67 (1993), S. 183-192 
    ISSN: 1570-7458
    Keywords: symbionts ; thelytoky ; arrhenotoky ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microbe-associated parthenogenesis (thelytoky) has been discovered in nineTrichogramma species, parasitoids of mainly lepidopteran eggs. Parthenogenetic and bisexual conspecifics co-occur in many field populations. As an initial step to understand the dynamics of these two reproductive strategies we studied the effect of microbe-associated parthenogenesis on fecundity. The fecundity of two parthenogenetic isofemale lines ofT. pretiosum and one ofT. deion was compared with bisexual lines derived from them by antibiotic treatment. In all three cases parthenogenetic females were less fecund over their lifetime than bisexual females. Also, parthenogenetic females produced fewer daughters in two cases and in one case a similar number of daughters as their respective bisexual counterparts. The lack of mating and insemination was excluded as an explanation for the reduced fecundity of parthenogenetic females, because mated and virgin parthenogenetic females produce the same number of offspring. Antibiotic treatment can also be excluded because females of field-collected bisexual line treated with antibiotics produced the same number of offspring as untreated females. The reduced fecundity of parthenogenetic females was caused by a lower number of eggs being laid rather than by a greater developmental mortality. Parthenogenetic females produced less daughters than bisexual females when host availability was not limiting, but when host availability was severely limited, parthenogenetic females produced more daughters than the bisexual females.
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  • 87
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    Entomologia experimentalis et applicata 67 (1993), S. 233-239 
    ISSN: 1570-7458
    Keywords: inbreeding ; colonization ; isofemale line ; Drosophila ; Diptera ; Leptopilina boulardi ; Cynipidae ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé D. melanogaster (Meigen) a été utilisé pour tester la capacité des lignées isofemelles à conserver la variabilité génétique d'une population naturelle. Deux types d'expériences ont été réalisées. L'une a consisté à déterminer la variabilité génétique de 3 locus enzymatiques pour 32 lignées isofemelles à la première et à la 23ème génération d'élevage au laboratoire. L'autre a consisté à tester la capacité des larves à éliminer un parasitoïde par le processus d'encapsulation après 8 années d'élevage au laboratoire. D'une façon générale, certaines lignées isofemelles perdent de la variabilité durant les 23 générations de l'étude. Mais la fréquence globale des allèles reste inchangée si l'on considère l'ensemble des 32 lignées. Le seul allèle rare observé a également été conservé. Les modifications des fréquences allèliques à chacun des locus ont lieu de façon indépendante les unes des autres. La variabilité génétique d'un caractère biologique, la capacité des larves à encapsuler le parasitoïde, a également varié, mais elle a pu être restaurée à un niveau proche de la population initiale en rassemblant plusieurs individus de chacune des lignées.
    Notes: Abstract Drosophila melanogaster (Meigen) was used to test the power of isofemale lines in preserving genetic variability. We performed experiments in two ways. One series consisted of measuring the genetic variability for three enzymatic loci in 32 isofemale lines, in the first and 23rd generations of culture. In the second series, we tested the capacity of the larvae to eliminate a parasitoid by encapsulation after eight years of laboratory breeding. In general, individual isofemale lines appeared to change during the 23 generations of the study, but the global frequency of these alleles among the 32 isofemale lines stayed relatively unchanged. The only rare allele observed was also conserved. Changes in allozyme frequencies at any one locus were independent of those at other loci. Genetic variation of a biological trait, the capacity of the larvae to encapsulate a parasitoid, also changed, but it could be restored to a level close to that of the starting population by mass hybridizing together individuals of each line.
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  • 88
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    Entomologia experimentalis et applicata 66 (1993), S. 171-177 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; egg parasites ; parasitoid quality ; mass rearing ; biological control ; factitious host
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between the index of female's size, hind tibia length (HTL), and selected fitness parameters used in quality assessment of mass-rearedTrichogramma spp. (Hymenoptera, Trichogrammatidae) was investigated. The studied fitness parameters were: the number of mature eggs present in the ovaries of females 0–2 hours, as well as 1, 2 and 3 days old, lifetime fecundity, fecundity during a 24 h period, longevity in the presence and absence of the factitious host (Ephestia kuehniella Zeller) and locomotor activity. The relationship between HTL and fitness parameters was analysed in 7 strains ofTrichogramma spp. (Trichogramma dendrolimi Matsumura,T. evanecens Westwood,T. maidis Pinturea et Voegelé andT. ostriniae Pang et Chen) using linear correlation. No significant relationship was found between HTL and egg complement, fecundity, longevity and locomotor activity. The results indicate that HTL used as an index of female's size is not suitable for quality assessment ofTrichogramma reared onE. kuehnieIla.
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  • 89
    ISSN: 1572-8889
    Keywords: Hymenoptera ; Leptopilina ; Drosophila ; semiochemicals ; kairomones ; parasitoid ; generalist ; specialist ; foraging behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Foraging parasitoids are thought to need more specific information than generalists on the presence, identity, availability, and suitability of their insect host species. In the present paper, we compare responses to host kairomones by two phylogenetically related parasitoid species that attack Drosophilidae and that differ in the width of their host range. As predicted, the behavioral response of the parasitoids to host kairomones reflected their difference in host range. The response of the specialist parasitoid Leptopilina boulardiwas restricted to contact kairomones from its natural hosts and one closely related species. In contrast, the generalist parasitoid Leptopilina heterotomaresponded to contact kairomones of a variety of Drosophilidae species.
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  • 90
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    Journal of insect behavior 6 (1993), S. 737-750 
    ISSN: 1572-8889
    Keywords: sex pheromone ; vibrational communication ; courtship sequence ; Hymenoptera ; Braconidae ; Cotesia rubecula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The courtship behavior of the parasitic wasp Cotesia rubeculawas studied in a flight tunnel using standard quantitative ethological techniques. Emission of a female sex pheromone induces searching and signaling behavior in males. Males combine wing-fanning, which produces low-frequency airborne sound, with “pulsing” behavior, which transmits a vibrational signal through the substrate to the female and induces her receptivity. Female receptivity is indicated by a stereotyped antennal position, which may provide a visual or tactile signal to courting males. Comparison of successful and unsuccessful courtships indicated that courtship success was dependent primarily on the effective production or reception of the male pulse signal. Overall, the sequence of courtship behavior was similar to that reported for other parasitic wasps.
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  • 91
    ISSN: 1572-8889
    Keywords: Phoridae ; Diptera ; Formicidae ; Hymenoptera ; host ; parasitoid ; behavior ; Atta ; Neodohrniphora
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    Topics: Biology
    Notes: Abstract This study examines the oviposition behavior of the phorid parasitoid Neodohrniphora curvinervisand the antiparasitoid defense behavior of its leafcutting ant host Atta cephalotes. N. curvinervisfemales are diurnal sit- and- wait parasitoids that attack only outbound foragers of head width 1.6 mm or greater. Females deposit a single egg through the foramen magnum of each host successfully parasitized. Pursuit of hosts is usually initiated when an outbound forager of acceptable size passes by a parasitoid perch site. Individual foragers defend themselves against pursuing parasitoids by outrunning them along the foraging trail or by standing their ground and fending them off with their legs,antennae, and mandibles. At the colony level, susceptible foragers are protected against parasitism by a shift in the forager size distribution toward smaller unsusceptible sizes during the day when parasitoids are active and toward larger sizes at night when parasitoids are inactive. The frequency of parasitism of susceptible foragers was 15%, which is more than five times the frequency found in another system involving the phorid parasitoid Apocephalus attophilusand the leafcutting ant host Atta colombica.We offer several possible explanations for such differences in the frequency of parasitism and also examine reasons for the high incidence of superparasitism (19%) observed in the system studied.
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  • 92
    ISSN: 1572-8889
    Keywords: Hymenoptera ; Braconidae ; Microplitis croceipes ; cotton ; cowpea ; parasitoid ; host location ; kairomones ; olfaction ; induced plant responses ; learning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study was conducted to determine the primary source of volatile cues within the plant-host complex used by hostseeking freeflying female Microplitis cro-ceipesCresson in flight tunnel bioassays. In single-source and two-choice tests, using wasps given an oviposition experience on either cotton (Gossypium hirsutum)or cowpea (Vigna unguiculata)seedlings damaged by corn earworm (CEW; Helicoverpa zeaBoddie), the damaged seedlings were significantly more attractive than the CEW frass, which was in turn more attractive than the larvae themselves. In a series of two-choice wind-tunnel tests, the discriminatory ability of the wasps was examined, following various oviposition experiences. Significantly more wasps flew to plants with “old” damage than to plants with “fresh” damage, regardless of whether they had experience on fresh or old damage. In a comparison of plant species, wasps with only one experience on either hostdamaged cotton or host-damaged cowpea were unable to distinguish between them, and showed no preference for either plant, whereas wasps with multiple experiences on a particular plant preferentially flew to that plant in the choice test. In comparing hosts with nonhosts, wasps successfully learned to distinguish CEW from beet armyworm (BAW; Spodoptera exigua)on cotton but were unable to distinguish CEW from either BAW or cabbage looper (Trichoplusia ni)on cowpea. The results show the important role played by plant volatiles in the location of hosts by M. croceipesand indicate the wasps’ limitations in discriminating among the various odors. The ecological advantages and disadvantages of this behavior are discussed.
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  • 93
    ISSN: 1572-8889
    Keywords: brood ; pheromone ; slavemaking ants ; Polyergus breviceps ; Formica occulta ; Hymenoptera ; Formicidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Freeliving workers of Formica occulta, an ant species enslaved by the obligatory slavemaking ant Polyergus breviceps, retrieve and nurse Polyergus pupaejust as well as conspecific pupae in a choice test. No such attraction was found toward pupae of the facultative slavemaker; Formica wheeleri,which also enslaves F. occulta. Formica neogagates,a sympatric species which is not parasitized by either slavemaker, preferentially retrieves and tends conspecific brood over that of Polyergusand F. wheeleri.It is proposed that brood of obligatory slavemaking species must possess an attractive pheromone for slavemaker colony foundation to be successful, since slavemaker brood must be nursed by adult slave workers with no prior exposure to slavemaker brood. An attractive pheromone is not necessary in the brood of facultative slavemakers, since this brood is cared for by newly eclosed slave workers who imprint on the slavemaker brood.
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  • 94
    ISSN: 1420-9071
    Keywords: Ropalidia marginata ; primitively eusocial wasp ; queen success ; worker-brood genetic relatedness ; inclusive fitness theory ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ropalidia marginata is a primitively eusocial polistine wasp in which, although there is only one queen at any given time, frequent queen replacements lead to a system of serial polygyny. One of the most striking features of this system, is the enormous variation in the success of different queens. Measuring queen success as queen tenure, total number of offspring produced, number of offspring produced per day of tenure, and proportion of eggs laid that develop into adults, we show here that each measure of queen success is correlated with worker-brood genetic relatedness and not correlated with worker: brood ratio or the age of the queen at takeover. We interpret these results as meaning that queens are better able to obtain the cooperation of workers when worker-brood genetic relatedness is high.
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  • 95
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    Entomologia experimentalis et applicata 66 (1993), S. 31-38 
    ISSN: 1570-7458
    Keywords: Eurytoma amygdali ; Hymenoptera ; Eurytomidae ; egg distribution ; superparasitism ; host discrimination ; oviposition behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The egg distribution patterns ofEurytoma amygdali Enderlein (Hymenoptera, Eurytomidae), which oviposits singly in green, developing almonds, were studied in the laboratory and in the field. In the laboratory, individual females were caged with a number of almonds and the eggs deposited in each fruit were counted. In the field, eggs were censused in almonds of different varieties at regular intervals, over four seasons (1988–91). In the susceptible ‘Retsou’ variety, eggs were uniformly distributed among fruits, both in the laboratory and in the field, as long as the mean number of eggs per almond was ≤2.5. When the mean number of eggs per almond was higher the egg distributions were random. This suggests that, up to a certain level of infestation, females were able to assess egg load of fruits and oviposit in the less infested ones. The main factor enabling the females to discriminate and select the less infested almonds for oviposition is probably a host-marking pheromone. In 1990, the same patterns of egg distribution were observed in samples taken from 5 other almond varieties that are not as susceptible as Retsou and have thicker pericarp and harder endocarp. In 1991 however, when fruits developed more rapidly than in 1990, egg distributions in 3 of these 5 varieties (Truoito, Ai, and Marcona) were not uniform. Although the mean number of eggs per fruit was low (1.2–2.0), many fruits of these varieties contained no eggs. This suggests that, in some less susceptible varieties, egg distribution might also be affected by certain fruit parameters, unfavourable for oviposition, related to the pericarp thickness and endocarp hardness.
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  • 96
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    Journal of comparative physiology 172 (1993), S. 207-222 
    ISSN: 1432-1351
    Keywords: Insects ; Hymenoptera ; Homing ; Visual ; spatial memory ; Landmark orientation ; Orientation flights
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cerceris wasps learn the position of their nest relative to landmarks during the performance of orientation flights. This paper examines the similarities that exist between an orientation flight made on departing from the nest and the subsequent return flight to the nest area. Returning wasps do not exactly retrace the paths they have flown during the preceding orientation flight. But there are striking similarities: in both types of flight wasps face into similar directions and their orientation depends on their position relative to nest and landmarks in a similar way. During both orientation flights and returns wasps fly along arcs while counter-turning at similar angular velocities. In both flights their flight direction and the retinal position of close landmarks are similar. Wasps on their return thus experience much the same spatio-temporal pattern of visual stimulation on their retina as they generated during their previous orientation flight. To discover whether wasps exploit the motion parallax information produced by these flights, the arrangement and size of landmarks was altered between the insects' departure and their return. Their search pattern for the hidden nest indicates (i) that they weight close landmarks more heavily than distant ones and (ii) that they frequently search at the appropriate distance from a landmark regardless of its apparent size. Both findings imply that returning wasps recall the patterns of visual motion produced during their orientation flights.
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  • 97
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    Journal of comparative physiology 172 (1993), S. 189-205 
    ISSN: 1432-1351
    Keywords: Insects ; Hymenoptera ; Homing ; Visual spatial memory ; Landmark orientation ; Orientation flights
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Bees and wasps are known to use a visual representation of the nest environment to guide the final approach to their nest. It is also known that they acquire this representation during an orientation flight performed on departure. A detailed film analysis shows that orientation flights in solitary wasps of the genus Cerceris consist of a systematic behavioural sequence: after lift-off from the nest entrance, wasps fly in ever increasing arcs around the nest. They fly along these arcs obliquely to their long axis and turn so that the nest entrance is held in the left or right visual field at retinal positions between 30° and 70° from the midline. Horizontal distance from the nest and height above ground increase throughout an orientation flight so that the nest is kept at retinal elevations between 45° and 60° below the horizon. The wasps' rate of turning is constant at between 100°/s and 200°/s independent of their distance from the nest and their ground velocity increases with distance. The consequence of this is that throughout the flight wasps circle at a constant angular velocity around the nest. Orientation flights are strongly influenced by landmark lay-out. Wasps adjust their flight-path and their orientation in a way that allows them to fixate the nest entrance and to hold the closest landmark in their frontal visual field. The orientation flight generates a specific topography of motion parallax across the visual field. This could be used by wasps to acquire a series of snapshots that all contain the nest position, to acquire snapshots of close landmarks only (distance filtering), to exclude shadow contours from their visual representation (figure-ground discrimination) or to gain information on the distance of landmarks relative to the nest.
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  • 98
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    Chemoecology 4 (1993), S. 29-32 
    ISSN: 1423-0445
    Keywords: chemical defence ; alkaloids ; predation ; Coleoptera ; Coccinellidae ; Adalia bipunctata ; Coccinella septempunctata ; Hymenoptera ; Formicidae ; Lasius niger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Garden black ants,Lasius niger L., in a laboratory colony, attacked three species of live ladybirds found near their nest, killing the smaller two species. A second colony was offered artificial diets containing crushed ladybirds of two species, and the ants' choice of feeding site noted. Both the diets were aversive compared to control, but that containing 7spot,Coccinella septempunctata L., was more aversive than the diet containing 2spot,Adalia bipunctata L. The implications of this lesser protection for 2spots in terms of the chemical defence of the species are discussed.
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  • 99
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    Chemoecology 4 (1993), S. 125-144 
    ISSN: 1423-0445
    Keywords: pheromones ; exocrine glands ; behaviour ; trail pheromone ; Dufour gland ; poison gland ; pygidial gland ; chemotaxonomy ; Hymenoptera ; Formicidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chemical communication plays a very important role in the lives of many social insects. Several different types of pheromones (species-specific chemical messengers) of ants have been described, particularly those involved in recruitment, recognition, territorial and alarm behaviours. Properties of pheromones include activity in minute quantities (thus requiring sensitive methods for chemical analysis) and specificity (which can have chemotaxonomic uses). Ants produce pheromones in various exocrine glands, such as the Dufour, poison, pygidial and mandibular glands. A wide range of substances have been identified from these glands.
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  • 100
    ISSN: 1432-041X
    Keywords: Oogenesis ; Accessory nuclei ; Morphogenetic signals ; Hymenoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphogenesis of accessory nuclei (AN) in chorionated oocytes of Cosmoconus meridionator is described. Initially, each AN contains two dense, morphologically distinct inclusions. During the final stages of postvitellogenesis, these inclusions undergo characteristic transformation that is followed by the extrusion of some substances from AN to the surrounding periplasm. Histo- and cytochemical tests indicate that both inclusions contain RNA, although their precise composition is different. Our results support previous suggestions on the involvement of AN in the distribution of morphogenetic signals.
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