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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 2
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A field experiment was conducted to assess the progress in time and spread in space of powdery mildew (caused by Erysiphe pisi) in pea (Pisum sativum) cultivars differing in resistance to the disease. Disease severity (proportion of leaf area infected) was measured in 19 × 23 m plots of cultivars Pania and Bolero (both susceptible) and Quantum (quantitatively resistant). Inoculum on infected plants was introduced into the centre of each plot. Leaves (nodes) were divided into three groups within the canopy (lower, middle, upper) at each assessment because of the large range in disease severity vertically within the plants. Disease severity on leaves at upper nodes was less than 4% until the final assessment 35 days after inoculation. Exponential disease progress curves were fitted to disease severity data from leaves at middle nodes. The mean disease relative growth rate was greater on Quantum than on Pania or Bolero, but it was delayed, resulting in an overall lower disease severity on Quantum. Gompertz growth curves were fitted to disease progress on leaves at lower nodes. Disease progress on Quantum was delayed compared with Pania and Bolero. The average daily rates of increase in disease severity from Gompertz curves did not differ between the cultivars on these leaves. Disease gradients in the plots from the inoculum focus to 12 m were detected at early stages of the epidemic, but the effects of background inoculum inputs and the rate of disease progress meant that these gradients decreased with time as the disease epidemic intensified. Spread was rapid, and there were no statistically significant differences between cultivar isopathic rates (Pania 2.2, Quantum 2.9 and Bolero 4.0 m d−1).
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Sweet potato virus disease (SPVD) is the name used to describe a range of severe symptoms in different cultivars of sweet potato, comprising overall plant stunting combined with leaf narrowing and distortion, and chlorosis, mosaic or vein-clearing. Affected plants of various cultivars were collected from several regions of Uganda. All samples contained the aphid-borne sweet potato feathery mottle potyvirus (SPFMV) and almost all contained the whitefly-borne sweet potato chlorotic stunt closterovirus (SPCSV). SPCSV was detected by a mix of monoclonal antibodies (MAb) previously shown to react only to a Kenyan isolate of SPCSV, but not by a mixture of MAb that detected SPCSV isolates from Nigeria and other countries. Sweet potato chlorotic fleck virus (SPCFV) and sweet potato mild mottle ipomovirus (SPMMV) were seldom detected in SPVD-affected plants, while sweet potato latent virus (SPLV) was never detected. Isolates of SPFMV and SPCSV obtained by insect transmissions together induced typical symptoms of SPVD when graft-inoculated to virus-free sweet potato. SPCSV alone caused stunting and either purpling or yellowing of middle and lower leaves when graft-inoculated to virus-free plants of two cultivars. Similarly diseased naturally inoculated field plants were shown consistently to contain SPCSV. Both this disease and SPVD spread rapidly in a sweet potato crop.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four experiments studied the effects of a clover understorey on pycnidiospore dispersal of Septoria tritici in a wheat–clover intercrop under simulated rain. Clover significantly reduced the dispersal of spores in a horizontal direction by 33% at a distance of 15 cm from a line inoculum source compared with a wheat monocrop. The clover also reduced the vertical movement of spores from infected leaves at the base of wheat plants by an average of 63% compared to the monocrop, and this suggests that the main movement of spores was from the base upwards. Splash experiments using blue colour marker showed the vertical decline of splash and the number of drops per cm2 with height caught on paper adjacent to trays of clover was described by the exponential decline model. The effect of clover in reducing vertical splash approached an asymptote as the leaf area index of the understorey increased. Simulated rain-splash increased the level of disease on the flag leaf and, in one experiment, there was a significant interaction between rain-splash and clover in reducing the number of lesions on the flag leaf. The level of disease resulting from one splash event was low, indicating that subsequent pathogen multiplication is probably required to bring about high severities of disease.
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Thrips species and tomato spotted wilt virus (TSWV) alternate weed hosts were surveyed on two lettuce farms in southern Tasmania during 1994 and 1995. Only one known vector species, Thrips tabaci, was found at either site, comprising on average 36.8% of the total monthly catch. A major peak of thrips activity in the summer corresponded with an increase of disease in autumn harvested lettuce. Two thrips species new for Tasmania were recorded, Pseudanaphothrips achaetus and Tenothrips frici. Infection patterns within the crop indicated that localized weed infestations were the most likely reservoir of virus. ELISA testing showed that TSWV was present in a range of dicotyledonous weed species, although usually infecting only a low percentage of the plants. Arctotheca calendula appeared to be the single most important reservoir host species at one property, whilst this species and Sonchus oleraceus, Malva sylvestris, Brassica rapa ssp. silvestris, Erodium moschatum and Trifolium sp. were probably the most important reservoirs at the other property. Two new natural TSWV host species were recorded, Erodium moschatum and Brassica rapa ssp. silvestris. The property with the highest incidence of TSWV-infected lettuce had a relatively higher proportion of virus-infected weeds but less thrips activity during the infection period.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Models of a banana bunchy top virus disease epidemic were developed to incorporate the two key features of an epidemic in a plantation in the Philippines: an exponential increase in disease incidence over 10 years, and a declining gradient of incidence from the outside edge of the plantation to the centre. A non-spatial model consisted of three difference equations to describe the numbers of latently infected and of infectious plants in the plantation and the size of the inoculum source outside the plantation. In a spatial model the outside portion of the plantation was divided into eight blocks running parallel to the outside edge. The dispersal gradient of the inoculum was assumed to be negative exponential. Analysis of the two models showed that for disease incidence to increase exponentially over time, the rate of disease progress could be dependent either on internal spread and roguing rate (proportion of diseased plants removed and replaced per unit time) or on the rate of increase of external inoculum pressure. The observed incidence gradient from the edge to the centre of the plot could be explained only if external inoculum dominated the parameters in the spatial model. This model was also used to explore a variable roguing rate across blocks. Simulations indicated that this may produce small gains over the adoption of a constant roguing rate over all blocks, but was risky because a shift of roguing emphasis only slightly too far towards the outside blocks can result in a dramatic increase in disease.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 11
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The infection of tomato fruit by the postharvest pathogen Colletotrichum gloeosporioides did not proceed until the onset of ripening in response to ethylene. Compared with fruit from wild-type plants, infection progressed more slowly in transgenic fruit in which ethylene biosynthesis and ripening had been inhibited by an ACC oxidase (ACO) antisense transgene. In contrast, transgenic fruit deficient in polygalacturonase developed lesions at the same rate as the wild-type fruit. Ethylene biosynthesis increased rapidly in response to infection of ripe wild-type and ACO antisense fruit but was 25 times greater in the former. Fruit from the ripening mutant ripening inhibitor (rin), which are normally very resistant to infection, became infected quickly when incubated in the presence of ethylene, whereas fruit incubated in the absence of ethylene remained healthy. The ACO 1 mRNA accumulated to detectable levels within 24 h of inoculation of unripe wild-type fruit, prior to the development of visible symptoms, whereas there was no detectable expression in the inoculated ACO antisense fruit. ACO transcripts accumulated to maximum levels during the early stages of infection of ripe fruit, correlating with maximum ethylene biosynthesis. Northern analysis using gene-specific probes for each of the three ACO genes indicated that ACO 1 was the main gene expressed in response to infection and that there was no detectable expression of ACO 2 and 3.
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  • 12
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Winter oilseed rape grown in several areas in South Bohemia showed symptoms of stunting, leaf reddening and extensive malformation of floral parts. Phytoplasmas were consistently observed by using electron microscopy only in phloem tissue of symptomatic plants. DNA isolated from infected and healthy control plants was used in PCR experiments. Primer pairs R16F2/R2, P1/P7 and rpF2/R2, amplifying, respectively, 16S rDNA, 16S rDNA plus spacer region and the beginning of the 23S and ribosomal protein gene L22 specific for phytoplasmas, were used. According to RFLP and sequence analyses of PCR products, the phytoplasma from rape was classified in the aster yellows phytoplasma group, subgroup 16SrI-B. The PCR products from the Czech phytoplasma-infected rape also had RFLP profiles identical to those of phytoplasma strains from Italian Brassica. This first molecular characterization of phytoplasmas infecting rape compared with strains from Brassica does not, however, clearly indicate differences among isolates of the same 16SrI-B subgroup. Further studies on other chromosomal DNA portions could help the research on host specificity or on geographical distribution of these phytoplasmas.
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  • 13
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Pseudomonas fluorescens F113 and Stenotrophomonas maltophilia W81 protect sugar beet from Pythium-mediated damping-off through production of the antifungal secondary metabolite 2,4-diacetylphloroglucinol and extracellular proteolytic activity, respectively. In this study, the two biocontrol strains were combined in a consortium, with the objective of improving upon the level of protection achieved when using each strain singly. Growth and in vitro production of 2,4-diacetylphloroglucinol by F113 and extracellular lytic enzymes by W81 were not affected when inoculated in combination. The abilities of W81 and F113 to colonize the rhizosphere of sugar beet were essentially similar when the two strains were applied singly or coinoculated onto seeds in a 1 : 1 ratio, both in natural soil microcosms and under field conditions. Concomitantly, single inoculation with W81 or F113 effectively prevented colonization of sugar beet seeds by Pythium spp. in soil microcosms, without the necessity for combining both strains. However, this parity was not reflected in seed emergence where the combination of W81 and F113 significantly enhanced final sugar beet stands (to the level achieved with chemical pesticides) under microcosm conditions at 28 days after sowing. In a field experiment, the only inoculation treatment capable of conferring effective protection of sugar beet was that in which W81 and F113 were coinoculated, and this treatment proved equivalent to the use of chemical fungicides. In conclusion, when compared with single inoculations of either biocontrol strain, the combined use of a phloroglucinol-producing P. fluorescens and a proteolytic S. maltophilia improved protection of sugar beet against Pythium-mediated damping-off.
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  • 14
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Powdery mildew infection of barley with the mlo5 barley powdery mildew resistance gene was examined, using near-isogenic barley lines, with and without mlo5 resistance, and two near-isogenic powdery mildew isolates, HL3/5 and GE3 with high (virulent) or low (avirulent) penetration efficiency on the resistant barley line. In all isolate–host combinations (except GE3 on the resistant barley line), frequency of haustorium formation increased significantly from zero at 11 h after inoculation to a maximum by 13 h, and there was no subsequent increase up to 24 h. In the susceptible barley line, 27% of appressoria from both isolates formed haustoria. Although this was significantly higher than the frequency of haustorium formation (18%) of HL3/5 on the resistant barley line, HL3/5 was much more successful than GE3 (frequency of haustorium formation less than 1%). The fact that HL3/5 did not possess a generally higher ability to penetrate successfully to form haustoria on the susceptible barley line, indicates that HL3/5 did not overcome the mlo5 resistance by being generally more vigorous. In the resistant barley line, papillae were larger than in the susceptible line; however, both isolates were associated with papillae of the same diameter at the time of penetration. We suggest that the mlo5 resistant barley line confers two different forms of resistance: isolate-specific and isolate-nonspecific.
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  • 15
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 16
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Factors affecting the production of conidia of Peronosclerospora sorghi, causing sorghum downy mildew (SDM), were investigated during 1993 and 1994 in Zimbabwe. In the field conidia were detected on nights when the minimum temperature was in the range 10–19°C. On 73% of nights when conidia were detected rain had fallen within the previous 72 h and on 64% of nights wind speed was 〈 2.0 m s−1. The time period over which conidia were detected was 2–9 h. Using incubated leaf material, conidia were produced in the temperature range 10–26°C. Local lesions and systemically infected leaf material produced 2.4–5.7 × 103 conidia per cm2. Under controlled conditions conidia were released from conidiophores for 2.5 h after maturation and were shown to be well adapted to wind dispersal, having a settling velocity of 1.5 × 10−4 m s−1. Conditions that are suitable for conidia production occur in Zimbabwe and other semi-arid regions of southern Africa during the cropping season.
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  • 17
    Electronic Resource
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    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Stem segments from Mexican lime, sweet orange, grapefruit, Citrus excelsa and alemow, infected with five citrus tristeza virus (CTV) isolates, were cultured in vitro. Regeneration of roots and shoots were modified as a result of infection. The effect of CTV on the morphogenesis of stem segments cultured in vitro depended on the CTV isolate and the plant host, and showed a correlation with the in vivo effects observed in biological indexing. Evaluation of the morphogenic response of stem segments of Mexican lime and grapefruit can be used as an additional tool for the biological characterization of CTV isolates. The symptoms on sweet orange plants obtained from regenerated shoots indicated that CTV is unevenly distributed in the host plant cells and that the regeneration process may be utilized as a tool to separate strains from complex field isolates.
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  • 18
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Monocyclic components (development rate during the incubation period or latent period, lesion density, lesion size and disease severity) of rust (Uromyces appendiculatus) and of angular leaf spot (Phaeoisariopsis griseola) in two bean (Phaseolus vulgaris) cultivars (Rosinha G-2 and Carioca), pre-infected or not with bean line pattern mosaic virus (BLPMV), were determined. Trials were conducted at temperatures in the range from 9 to 27°C for rust and from 12 to 30°C for angular leaf spot. Regardless of viral pre-infection, the effect of temperature on the four monocyclic components followed an optimum curve and could be described by a generalized beta function. Generally, angular leaf spot was favoured by higher temperatures with an optimum for disease severity between 24.2 and 28.3°C compared with 15.9–18.5°C for rust. Pre-infection with BLPMV did not change the shape of the optimum curves for all components, but significantly reduced lesion density and disease severity on both cultivars. The development rates during incubation and latent periods for both fungal diseases were not affected by BLPMV. Pre-infection with virus did not alter the ranking of cultivars with respect to resistance to both fungal diseases.
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  • 19
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four populations of Pratylenchus thornei from different locations were tested for reproductive fitness in axenic carrot disc cultures and for pathogenicity to chickpea cultivars JG 62 and UC 27 and lines K 850 and ILC 1929. Parasitism and histopathology on selected chickpea genotypes (JG 62, UC 27 and lines ILC 482, ICC 11324 and ICC 12237) were also investigated. Reproductive fitness, assessed as the ratio of the final number of nematodes per carrot disc to the number of nematodes inoculated, was similar among the populations tested and the four populations reproduced to a similar extent in a given chickpea genotype. However, the extent of reproduction was significantly affected by the chickpea genotype, JG 62 and UC 27 being the best and poorest hosts, respectively. Pathogenicity to chickpea genotypes was assessed by the difference in fresh root and dry shoot weights between infected and uninfected plants 90 days after inoculation. Plant growth was significantly reduced by the four nematode populations in all chickpea genotypes, with the exception of cv. JG 62, which was tolerant of P. thornei. Severity of root necrosis caused by nematode infection was similar for all populations. Histopathological studies of chickpea genotypes infected by P. thornei showed that all were suitable hosts according to nematode reproduction and host reaction. P. thornei always migrated through epidermal and cortical cells by breaking down cell walls along the nematode pathway. In the most susceptible lines (ILC 482 and JG 62), damage to endodermal cells adjacent to nematode feeding sites was occasionally observed.
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  • 20
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In situ strand growth and sclerotium formation of Phymatotrichopsis omnivora were observed in minirhizotrons using a microvideo camera. Strand growth was greater in soils subject to decreasing levels of soil matric potential (high water stress) compared with a continuously wet treatment. Intermediate levels of water stress (−0.5 to −0.9 MPa) resulted in less strand growth than either the dry or wet treatments. In all treatments strand growth increased to a maximum within 10 days of inoculum placement in soil but then declined to approach an apparent steady-state value. The effects of soil matric potential on sclerotium formation were apparent when barley seeds were sown in the experimental units 3 weeks after the experiment had begun, permitting differential water cycling patterns to be obtained. Sclerotia were recovered in larger numbers from experimental units in which high water potentials (〉 −0.2 MPa) occurred 2 weeks prior to and following the death of host plants.
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  • 21
    Electronic Resource
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    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A wind tunnel was designed to study the effect of wind, relative humidity, leaf movement and colony age on dispersal of conidia of Uncinula necator. Wind speed as low as 2.3 m s−1 instantaneously triggered dispersal of conidia from fixed leaf discs of 18-day-old infections. Conidia were observed on sporulating leaf discs even after exposure to 17 m s−1 wind. The fraction of conidia dispersed at a given wind speed increased with colony age from 12 to 24 days. Conidia of 27-day-old colonies were less easily dispersed. No gradient of maturation of conidia along the conidial chain was observed, suggesting that even newly formed conidia were able to germinate after dispersal. Germination of dispersed conidia decreased slightly with greater colony age. Both wind and simulated rain drops caused dispersal of conidia from infected leaves. Leaf movement at wind speed of 3.5–4 m s−1 increased dispersal, and the first impact of three simulated raindrops caused release of 53% of the total conidia dispersed. Relative humidity had no effect on dispersal of conidia at different wind speeds.
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  • 22
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    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: During a survey of viruses of peanuts in South Africa a mechanically transmissible virus was isolated from a plant exhibiting chlorotic ringspots and blotches on the leaves. Typical potyvirus-like flexuous particles were detected by electron microscope examination. Pinwheel-shaped and laminated inclusions in ultrathin sections, reaction with a monoclonal antibody directed to a potyvirus common epitope, a single 33 kDa coat protein and aphid transmission using Myzus persicae all confirmed that the virus was a subdivision II member of the Potyviridae. Host range studies suggested that the virus was none of the previously reported potyviruses of peanuts or of subdivision II potyviruses. The serological relationships of the virus were studied using a range of 17 antisera to potyviruses in ELISA and immunosorbent electron microscopy (ISEM). The isolate reacted weakly with antisera to plum pox virus and bean yellow mosaic virus in ISEM only. Nucleotide sequence of a 624 bp DNA product was obtained following immuno-capture with a potyvirus common epitope antiserum, cDNA synthesis and PCR amplification with potyvirus specific primers which amplify the 3′ untranslated region and a part of the coat protein gene. The sequence was only distantly related to a number of potyviruses, whether amino acid or nucleotide sequences were used for comparisons. It is proposed that the virus be named peanut chlorotic blotch virus and be accepted as a new member of the genus Potyvirus in the family Potyviridae.
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  • 23
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    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Aerial spore concentration of Uncinula necator (the causal agent of grape powdery mildew), weather data and cropping practices were monitored during two consecutive seasons in two vineyards of the Bordeaux area. During days with no rain, spore dispersal was mainly diurnal and showed variations that followed the same pattern as that of wind speed, and a reverse pattern to that of relative humidity. Light falls of rain, of approximately 2 mm, coincided with increased spore densities in the air. Pesticide sprays using high pressure equipment generated high wind speeds at the canopy level. This may trigger high spore dispersal. High conidial stocks were produced under spontaneous conditions in the canopy. These stocks were released only under particular events, such as heavy rains, or pesticide applications with high pressure sprayers. Other cropping practices causing leaf shaking, such as pruning, may enhance spore dispersal. Over the observation period, the onset of spore dispersal was observed during a period with no rain following a rainy period, suggesting the detrimental effect of rains on epidemic onset. Epidemiological and disease management implications are discussed.
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  • 24
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    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Blast, caused by Pyricularia grisea, is a major constraint on rice production. To broaden genetic diversity for resistance to this disease, two rice cultivars, GA20 and GA25 from Yunnan Province, China, were analysed for the genetic basis of their high resistance to blast. GA20 was crossed with 10 Japanese differential cultivars, and GA25 was crossed with nine of them and with the susceptible Chinese cultivar Lijiangxintuanheigu (LTH). The resistance of GA20 was governed by two dominant genes allelic to genes at the Pi-k and Pi-ta loci. The allele at the Pi-k locus was new, based on a reaction pattern different from known alleles at this locus. It could not be shown whether or not the allele at the Pi-ta locus is new, because races with virulence for Pi-ta were not tested. GA25 has one resistance gene, which is not allelic to genes at the loci Pi-a, Pi-k, Pi-z, Pi-ta, Pi-b, and Pi-t, but is linked to the Pi-i gene on chromosome 9 with a recombination frequency of 15.1 ± 2.8%.The new allele at the Pi-k locus in GA20 is designated as Pi-kg(t), and the new resistance gene in GA25 as Pi15(t).
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  • 25
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    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Oospores of Phytophthora infestans were produced in potato leaf discs floating on metalaxyl solution (100 μg mL−1 a.i.) and inoculated with all combinations of two metalaxyl-sensitive and two -resistant parental isolates. Numbers of oospores produced varied between different matings, depending on parents, in the absence of the fungicide and when metalaxyl was added 0, 7, 14 and 21 days after inoculation. Oospores were not produced when metalaxyl was added at the time of inoculation (0 days) when either one or both parents were sensitive to metalaxyl. In two of three such matings further oospore formation was arrested when metalaxyl was added either 7 or 14 days after inoculation. Oospores extracted from leaf discs 14, 21 and 28 days after inoculation were assessed for germination on water agar after 21 days. Germination of oospores from water control treatments varied between 6 and 30% depending on the cross. Germination was significantly reduced in oospores of metalaxyl-sensitive parents extracted 28 days after inoculation of leaf discs treated with metalaxyl 0, 7 and 14 days after inoculation compared with the 21-day treatment. Minimal differences in germination were observed for oospores from the mating of resistant parents irrespective of metalaxyl treatment, although germination was generally low, not exceeding 8.5%.
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  • 26
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A reliable diagnostic method was developed for use in studying the relationship between phormium yellow leaf disease of New Zealand flax (Phormium tenax) and its associated phytoplasma (phormium yellow leaf phytoplasma: PYL). Diagnosis involved a nested PCR (polymerase chain reaction) technique targeting the 16S rRNA gene. DNA was extracted from woody rhizome tissues of NZ flax plants using CTAB and a high salt precipitation step. This method effectively eliminated polysaccharides, gum-like material and other compounds inhibitory to PCRs that occur at high concentrations in diseased NZ flax rhizomes. PCR competence of each DNA preparation from both healthy and yellow leaf diseased plants was assessed using the general prokaryotic 16S rRNA gene primers, Gd1/Berg54. These primers amplified DNA from both diseased and healthy plants. PYL 16S rDNA sequences were not detected consistently following amplification by PCR (35 cycles) using the ‘universal’ phytoplasma-specific primer pairs R16F2/R16R2 or P1/P6. By contrast, PYL was consistently detected in diseased, but not healthy, NZ flax plants, following nested PCR of the products of the above three primer pairs. Nested PCRs involve the primers NGF/NGR, which were designed to hybridize with all phytoplasmas for which published sequences were available. The most sensitive level of detection by nested PCR was achieved using primers R16F2/R16R2, rather than primers P1/P6 or Gd1/Berg54, for the primary amplification step. The consistent association found in this study between yellow leaf disease and PYL further substantiates this phytoplasma as the causal agent. PCR products of the expected size were also amplified by nested PCR using the primers R16F2/R16R2 followed by NGF/NGR from C. roseus tissues infected with five other phytoplasmas representing three distinct phytoplasma groups. Therefore nested PCRs with these pairs of primers should be useful for detecting other phytoplasmas, in particular those occurring at low concentrations or in recalcitrant tissues.
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  • 27
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A mechanically transmissible virus was isolated from a naturally infected alfalfa plant (Medicago sativa) in Karaj, Iran. It induced fern-leaf symptoms in Lycopersicon esculentum, general chlorosis and stunting in Arachis hypogoea, local lesions and systemic infection in Chenopodium quinoa, Phaseolus vulgaris and Vigna unguiculata but only local lesions in C. amaranticolor. In gel-immunodiffusion tests, it reacted strongly with an antiserum to peanut stunt cucumovirus (PSV), moderately with two of six antisera to cucumber mosaic cucumovirus (CMV) and with two of five antisera to tomato aspermy cucumovirus. Its spherical virions (28 nm in diameter) contained a coat protein of approximately 29 kDa and encapsidated four species of RNAs with similar electrophoretic mobilities to RNAs 1–4 of CMV strains Fny and LS and those of PSV strains J and W. Its encapsidated RNAs hybridized in slot-blot hybridization assay with the complementary DNA probe of PSV-W RNAs but not with those of CMV strains. Therefore, on the basis of biological, serological and physico-biochemical properties, the virus was identified as PSV. No satellite RNA was associated with the virus. This is the first report of PSV in Iran.
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  • 28
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The genetic relatedness of phytoplasmas associated with dieback (PDB), yellow crinkle (PYC) and mosaic (PM) diseases in papaya was studied by restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene and 16S rRNA/23S rRNA spacer region (SR). RFLP and SR sequence comparisons indicated that PYC and PM phytoplasmas were identical and most closely related to members of the faba bean phyllody strain cluster. By comparison the PDB phytoplasma was most closely related to Phormium yellow leaf (PYL) phytoplasma from New Zealand and the Australian grapevine yellows (AGY) phytoplasma from Australia. These three phytoplasmas cluster with the stolbur and German grapevine yellows (VK) phytoplasmas within the aster yellows strain cluster. Primers based on the phytoplasma tuf gene, which amplify gene products from members of the AY strain cluster, also amplified a DNA product from the PDB phytoplasma but not from either the PYC or PM phytoplasmas. Primers deduced from the 16S rRNA/SR selectively amplified rDNA sequences from the PDB and AGY phytoplasmas but not from other members of the stolbur strain cluster. Similarly, primers designed from 16S rRNA/SR amplified rDNA from the PYC and PM phytoplasmas but not from the PDB phytoplasma. These primers may provide for more specific detection of these pathogens in epidemiological studies.
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  • 29
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effect of two temperature regimes (daytime, 29 ± 2°C, night-time, 24 ± 3°C; and daytime, 23 ± 1°C, night-time, 18 ± 2°C) on the symptoms caused by tomato spotted wilt virus (TSWV), and the accumulation of TSWV virions, was compared in Datura stramonium, Nicotiana tabacum cv. White Burley and Physalis ixocarpa. Tobacco plants were more severely affected by TSWV at the high temperature regime, but the incidence (percent of plants with symptoms) was 100% for both regimes. In P. ixocarpa and D. stramonium the higher temperature caused an increase in both incidence and rate of development of symptoms. At high temperature, all three species showed both local and systemic symptoms; however, at low temperature only P. ixocarpa consistently developed systemic symptoms. In general, virus accumulation in the inoculated leaves (presumably the combined effect of virus replication and local movement) of all plants was higher at the lower temperature. Long distance movement in tobacco, leading to virion accumulation in other plant organs, was favoured by high temperature; but there was relatively little effect in P. ixocarpa and D. stramonium.
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  • 30
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two whitefly-transmitted viruses elicit identical symptoms of interveinal chlorosis (yellowing) in greenhouse- and field-grown cucurbits in Mediterranean countries. The agents of these diseases are partially characterized closteroviruses and are known as beet pseudo-yellows virus (BPYV), which is transmitted by Trialeurodes vaporariorum, and cucurbit yellow stunting disorder virus (CYSDV), transmitted by Bemisia tabaci. Using previously determined sequence information for fragments of the HSP70 homolog genes of both BPYV and CYSDV, oligonucleotide primers were designed for reverse transcription-polymerase chain reaction (RT-PCR) protocols to differentiate between the two viruses. RT-PCR amplified products for each virus were subsequently cloned and specifically hybridized with the homologous dsRNA (approximately 9 kbp in size for both viruses) following Northern hybridization analysis. Minor dsRNAs of approximately 5 kbp for both viruses and one dsRNA of 3 kbp for CYSDV were also identified by Northern hybridization and might represent defective RNA species. In order to compare the predicted amino acid sequence of fragments of the HSP70 homolog genes of BPYV and CYSDV, oligonucleotide primers were designed for RT-PCR amplification of a previously unknown segment of the BPYV gene from a Greek isolate and the American isolate of the virus. The RT-PCR products of both isolates were cloned and found to be identical in sequence. Computer assisted analysis of the deduced amino acid sequence of the assembled HSP70 homolog gene fragments showed that BPYV and CYSDV are closely related to each other and individually as closely related to the corresponding gene of lettuce infectious yellows virus, which is the prototype member of the recently proposed Crinivirus genus of whitefly-transmitted, bipartite closteroviruses.
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  • 31
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Existing PCR-based assays for the detection of the cereal pathogen Fusarium avenaceum were found to cross-react with F. tricinctum. An investigation into the phenetic relationship between these two species using two different marker systems revealed a close relationship between them despite their being from separate taxonomic sections. Whilst RFLP differences in the ITS regions surrounding the nuclear 5.8 S rDNA were insufficient to discriminate between isolates of the two species, they were clearly differentiated by RAPD profiling. RAPD fragments from F. avenaceum isolates were screened for hybridization to F. tricinctum DNA on Southern blots. One of 12 selected RAPD fragments showed no hybridization to genomic DNA from F. tricinctum. This fragment was cloned and sequenced, and the sequence obtained was used to design PCR primers. The primers were found to be specific for F. avenaceum, with no cross-reactions obtained with F. tricinctum or any other wheat pathogen assayed. The primers were able to differentiate between the two species in infected plant material, in contrast to the earlier assays.
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  • 32
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A pathogenicity survey of Puccinia recondita f.sp. tritici (Prt) was conducted in western Europe in 1995. Random urediospore isolates (850) of Prt were collected from the air by means of a jet spore sampler in wheat-growing regions of Austria, Belgium, France, Germany, northern Italy, Switzerland and the UK. Pathogenicity of the isolates was determined in tests of detached primary leaf segments maintained on water agar supplemented with benzimidazole (35 p.p.m.). The differential genotypes used were Thatcher, 20 near-isogenic Thatcher lines each with a single leaf rust resistance gene, and five cultivars/lines with additional resistance genes. All isolates were avirulent for the genes Lr9, Lr19, Lr21, Lr24, Lr25 and Lr29, and both virulence and avirulence were detected for the remaining 19 genes. Fifty-three pathotypes were identified, four of which predominated (64% of isolates) and were widespread throughout western Europe. Three of the four predominant pathotypes were also identified in collections of wheat leaf rust collected in Poland, Hungary, Estonia and Finland. One pathotype, which comprised 35% of isolates in the south of France, was not detected in any other region. This pathotype was indistinguishable from several isolates obtained from Morocco, which suggested that it may have originated from northern Africa. Comparisons with previously published data suggested that the four predominant pathotypes were very similar and possibly the same as pathotypes present in the former Czechoslovakia for up to 20 years. The results obtained provide evidence of migration of Prt over considerable distances in western Europe, stressing the need for a co-ordinated approach for genetical control of the disease in this region.
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  • 33
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Nine Myrobalan plum clones (Prunus cerasifera) obtained from in vitro propagation were tested for host suitability to four root-knot nematode (RKN) isolates (Meloidogyne incognita (2), M. javanica (1), and M. hispanica (1)) and one root-lesion nematode (RLN), Pratylenchus vulnus, isolate. The clones are characterized according to the Ma major resistance genes that control resistance to M. arenaria, M. incognita and M. javanica and are categorized into five resistant (R) and four host (H) clones to these RKN species. As expected, the clones ranged, regardless of the RKN species or isolate, into the same distinct R (P.2175, P.1079, P.2980, H.17 and H.21) and H (P.2794, H.18, H.23 and P.16.5) groups for RKN resistance. The P. vulnus tests showed that all the clones had higher final populations than initial populations (Pf : Pi 〉 1) and that Pf : Pi ratios were equivalent in each RKN resistance group (R or H). Consequently, resistant alleles of RKN Ma genes in Myrobalan plum have no major effect on RLN multiplication. Our data must be taken into account for breeding strategies aimed at incorporating multiple resistance to both RKN and RLN in Prunus spp.
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  • 34
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Polymerase chain reaction (PCR) assays for the detection of various Fusarium species and Microdochium nivale subspecies were compared with conventional visual disease assessment using a field plot of wheat in which the central subplot was inoculated with F. culmorum. Visual disease assessment was performed on a range of samples taken from each of 15 subplots at growth stage 80. At harvest, each sample was divided into its component parts, i.e. grain, glume and rachis, and species-specific PCR analysis was used to detect the presence of F. culmorum, F. poae, F. avenaceum, F. graminearum, M. nivale var. majus and M. nivale var. nivale. Within the inoculated subplot there was good correlation between visual disease assessment and PCR analysis, both techniques indicating a high incidence of F. culmorum in this region. According to the visual disease assessment results, there was also a relatively high incidence of F. culmorum in most other regions of the field plot. However, according to PCR analysis the incidence of F. culmorum in many of the other subplots was relatively low and F. poae, M. nivale var. majus and var. nivale, and F. avenaceum were detected within the grain, glume and rachis tissues of many of the ear samples from these subplots. F. poae predominated in the glume component of ears and M. nivale var. majus and var. nivale in the rachis component. M. nivale PCR results revealed that 64% of infected samples involved var. majus, and 36% var. nivale. PCR analysis has highlighted some difficulties that may arise when using visual assessment for studying disease complexes.
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  • 35
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Progenies from eight tetraploid potato crosses were assessed for the association between ineffective R-genes and lesion expansion rate (LER) in mm per day on detached leaves that had been inoculated with Phytophthora infestans. In two of the progenies, a significant association between ineffective R-genes and LER was measured, which could be an indication of residual effects. No association was found in the other progenies. These results and other corroborative evidence do not support an approach to plant breeding based on the stacking of R-genes to accumulate residual resistance effects. Using the same detached-leaf test, differences in aggressiveness between two isolates of P. infestans could be measured in all eight progenies. LER values were consistent with previously published ranges and were distributed continuously and unimodally in all progenies, suggesting that differences in relative aggressiveness of isolates were probably not affected by R-genes.
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  • 36
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The pathogenicity of Acremonium cucurbitacearum to 31 cucurbits, 18 crop plant species, and 15 weed species was evaluated under greenhouse conditions. This study demonstrates that many other cucurbits in addition to muskmelon and watermelon can potentially serve as hosts of A. cucurbitacearum. Using a disease severity index (DSI), all muskmelon and watermelon cultivars were ranked as susceptible or highly susceptible, with the exception of honeydew cultivar TAM Dew Improved, which ranked resistant in one study. Highly resistant cucurbits included Cucurbita maxima (autumn squash), Luffa acutangula, L. aegyptiaca (sponge gourd) and Benincasa hispida (ash gourd). Cyclanthera pedata and Cucumis miriocarpus were rated as resistant. The percentage recovery of A. cucurbitacearum ranged from 8% to 100% and tended to be higher in plants with a higher DSI. Other crops and weed species common to muskmelon production fields are not likely to be involved in the perpetuation or propagation of the fungus.
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  • 37
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Yellow net virus of sugar beet has, until now, been listed as a tentative member of the luteovirus genus, but this is the first study to show that the yellow net symptom (YN) is always associated with a luteovirus. This virus is related to beet mild yellowing virus (BMYV) and when transmitted alone causes a mild yellowing symptom similar to that caused by BMYV in beet. Plants with YN have been found to contain, in addition to the luteovirus, two prominent, low molecular weight double-stranded (ds)RNA species. This dsRNA is indicative of an extra component in a complex with the luteovirus, which gives rise to the conspicuous symptom. The range of experimental hosts for this virus has been extended to include Capsella bursa-pastoris and Physalis floridana.
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  • 38
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isolates of Pseudomonas syringae ssp. savastanoi from ash were examined for their ability to produce phytohormones in culture and for pathogenicity, in comparison with isolates from olive and oleander. Nineteen out of 20 ash isolates produced low levels of indole-3-acetic acid and its methyl ester but no cytokinins. In contrast, the remaining isolate, NCPPB3474, accumulated high levels of auxins and cytokinins in culture, comparable to those of olive and oleander strains. Hybridization of DNA preparations with tryptophan mono-oxygenase (iaaM) and isopentenyl transferase (ipt) gene-containing probes showed sequences of DNA homologous to both probes only in isolate NCPPB3474, and in which the iaaM and ipt genes were located on the chromosome and on a plasmid of about 80 kb, respectively. When assayed for pathogenicity on ash, olive and oleander, 19 of the 20 ash isolates caused disease only on ash but NCPPB3474 caused knots on both ash and olive. Oleander isolates infected all three hosts whereas those from olive caused symptoms only on olive and ash. All the cultures were able to multiply in host plant tissues, but the growth rates and final population densities were correlated to the plant species inoculated and the host origin of the isolates. In particular, the highest population densities were reached by isolates capable of causing symptoms on the inoculated host. The phytohormone production shown by ash, olive and oleander isolates of P. syringae ssp. savastanoi was in accordance with the type of symptoms: cankers accompanied by wart-like excrescences on ash and knots on olive and oleander. Furthermore, the pathogenic features of these isolates and, in particular, their growth patterns in the different host tissues, support previous evidence on the existence of three distinct pathovars in P. syringae ssp. savastanoi.
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  • 39
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of two grapevine rootstocks on susceptibility of grafted scions to crown gall (Agrobacterium vitis and Agrobacterium tumefaciens) was investigated in greenhouse and field experiments. The incidence of crown gall on a susceptible grape scion cultivar (Vitis vinifera, cv.  Kiralyleanyka) was not affected by grafting it on a crown gall resistant rootstock (Vitis riparia, cv.  Gloire) or on a susceptible rootstock (V. berlandieri × V. riparia, cv.  Teleki 5C) when the inoculated vines were observed over a 2-month period in the greenhouse. Weights of galls that were induced by six of eight strains of Agrobacterium (six A. vitis and two A. tumefaciens) were not significantly different, but A. tumefaciens strain 16/6 and A. vitis strain 2/3 produced significantly larger galls on Kiralyleanyka vines that were growing on their own roots or were grafted on 5C than on those grafted on Gloire. When the same vines were planted in the field and observed over a 4-year period, no differences in incidence of crown gall were appa_rent until the third and fourth years, when vines grafted on Gloire had less crown gall than vines on 5C. By that time, the natural symptoms (galls along the trunk) of the disease appeared, coinciding with the decline of whole vines. In another field experiment, with naturally infected scions of V. vinifera, cv.  Blau Frankisch (susceptible), crown gall was appa_rent on vines grafted on both rootstocks after 2 years, but in the fifth year 79% of the vines on 5C had crown gall whereas only 9% on Gloire showed symptoms. After the appearance of typical natural symptoms, vines showed reduced vigor with substantial loss of trunks and entire vines. During this period, many vines on 5C died as compared with only a few on Gloire. By the end of the sixth year, 13% of the originally planted vines on 5C and 82% on Gloire were alive, and although many pathogenic strains of Agrobacterium spp. were isolated from the roots of 5C, none was isolated from Gloire.
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  • 40
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The anamorphic state of a powdery mildew, which first appeared on tomato in the UK in 1987, currently listed as Erysiphe orontii (anamorph Oidium violae), was examined microscopically on both tomato and alternative hosts. Host range studies were also carried out. In 1993–95, species and cultivars of plants from 26 of the 30 families listed as hosts of E. orontii were inoculated with tomato powdery mildew in the glasshouse and assessed for susceptibility on a 0–3 scale based on degree of sporulation and growth. Thirteen families contained species or varieties that were susceptible and a total of 80 varieties, including many economically important Cucurbitaceae and Solanaceae, supported sporulation to some extent (susceptibility score 1–3). Necrosis was not a constant feature of plants exhibiting resistance, as it could accompany sporulation on some susceptible varieties. Microscopic measurements showed that conidial size, conidiophore length, conidiophore foot-cell length and width, and the number of cells distal to the foot-cell were remarkably constant between samples of powdery mildew taken from tomato each year and from the various alternative hosts. Based on the production of conidia singly, the presence of nipple-shaped to moderately lobed appressoria, and occurrence of straight conidiophores, it is concluded that this fungus on tomato should more correctly be named Oidium lycopersicum until the sexual stage is discovered. The highly polyphagous nature of this powdery mildew would suggest that it represents a potential risk to UK agriculture and horticulture, but the failure to recover any powdery mildew from 120 samples of potato, including varieties Cara and Maris Piper, on which the tomato powdery mildew could sporulate in the glasshouse, suggests that environmental factors may play a key role in preventing establishment of this powdery mildew on alternative hosts on a commercial scale in the field.
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  • 41
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The colonization of the roots of four cultivars of subterranean clover by isolates representing four races of Phytophthora clandestina was studied. There was a highly significant race × cultivar interaction in the growth of inoculated tap roots and the degree of colonization of roots by the pathogen. While all races were able to infect the roots of all cultivars tested, roots of the susceptible cultivars were colonized more rapidly and extensively than those of the resistant cultivars. In compatible combinations, fungal colonization extended for a few centimetres in the tap root and lateral roots in the moderately susceptible cultivars Trikkala and Meteora, or throughout the whole root system leading to the death of the host in the very susceptible cultivar Woogenellup. In contrast, limited fungal colonization of the tap root and lack of extension of the fungus into lateral roots was typical of incompatible combinations. In all cultivars, lateral roots were as susceptible to infection as tap roots. The number of lateral roots of Woogenellup was significantly reduced by infection. However, neither the rate of lateral root formation nor the total number of lateral roots of Seaton Park, Meteora and Trikkala was reduced by infection with virulent or avirulent races of the pathogen.
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  • 42
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Amplified fragment length polymorphism (AFLP) markers were used to determine genetic diversity and population structure of Pyrenopeziza brassicae, the causal agent of light leaf spot of Brassica spp. Fungal isolates were sampled from six regions in the UK, one region in Germany and one region in France. A high level of genetic diversity was found (HT = 58%), with most variation attributed to within regions (HS = 43%), which suggests that sexual reproduction is frequent. FST values suggested significant population differentiation between England and the continent, but not between Scotland and England and Scotland and the continent. Overall, a moderate but significant level of regional differentiation was found (FST = 16 ± 4.0). There was no correlation between FST values and distance, indicating that long-distance dispersal by natural factors does not occur at high frequencies. However, the lack of differentiation among populations from Aberdeen, Winchester and Cambridge suggests that seed transmission or other artificial methods of dispersal may be important.
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  • 43
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    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Monarda yellows occurring in southern Alberta was found to be associated with a phytoplasma. Using two pairs of universal primers, 16S ribosomal DNA fragments (about 1.5 and 1.2 kb) were amplified separately by polymerase chain reaction (PCR) from DNA samples that had been extracted from infected monarda. No such DNA bands were observed using DNA samples from uninfected monarda. The DNA fragment (1.2 kb) amplified by nested-PCR was analysed and compared with western aster yellows (AY27, Canada), eastern aster yellows (EAY, USA), French hydrangea aster yellows (AYHF), Belgium hydrangea aster yellows (AYHB), clover proliferation (CP, Canada) and potato witches'-broom (PWB, Canada) by means of restriction fragment length polymorphism (RFLP) using endonucleases AluI, MseI, HpaII, Sau3AI, KpnI and RsaI. The results showed that monarda yellows phytoplasma belongs to the aster yellows subclade and is different from CP and PWB. This is the first report of aster yellows phytoplasma infecting monarda.
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  • 44
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ninety-eight isolates of Rhizoctonia spp. were obtained from barley and wheat grown in Erzurum, Turkey. Of these, 78% were Rhizoctoniasolani (AG-2 type 1, AG-3, AG-4, AG-5 and AG-11), 10% were binucleate Rhizoctonia (AG-I and AG-K) and the remainder were Waitea circinata var circinata (Rhizoctonia sp.). Among the binucleate Rhizoctonia, AG-I was not recovered from barley. In pathogenicity tests on barley and wheat, the highest disease severity was caused by isolates of AG-4 and AG-11, whereas isolates of AG-2 type 1, AG-3, AG-5 and W. c. var circinata were moderately virulent. Isolates of binucleate Rhizoctonia were all nonpathogenic. This is the first report of R. solani AG-11 and W. c. var circinata from Turkey.
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  • 45
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isolates of Rhizoctonia solani AG2-2 obtained from turf with symptoms of large-patch disease of warm-season turfgrasses were compared with known AG2-2 isolates belonging to cultural types IIIB and IV. Some isolates that were previously identified as type IV have been separated here and named LP isolates. Comparisons among isolates were based on cultural morphology, hyphal growth rate, pathogenicity and restriction fragment length polymorphism (RFLP) analysis in the nuclear encoded ribosomal DNA (rDNA) genes. The cultural characteristics of LP isolates varied from those of types IIIB and IV. LP isolates did not show distinct sclerotial formation and zonation, and the colour of their mycelia and pigment deposition was dark brown. LP isolates had slower hyphal growth rates than types IIIB and IV, with an optimum temperature of 25°C compared with 28°C for types IIIB and IV. LP isolates were less virulent on radish but highly virulent on zoysia grass when compared with isolates of types IIIB and IV. Genomic DNA was digested separately with Eco RI, Ban III, Xba I and Sal I, and probed with cloned rDNA from Alternaria alternata in Southern hybridizations. LP isolates had one RFLP pattern, while both IIIB and IV possessed four different patterns each. Cluster analysis of RFLPs showed that R. solani AG2-2 is divided into three genetic subgroups, consisting of the IIIB, IV and LP isolates, respectively. The polymerase chain reaction (PCR) amplified rDNA internally transcribed spacer (ITS) regions of the IIIB, IV and LP isolates had the same length but produced different restriction patterns when digested with Msp I and Taq I. These results indicate that there are three cultural types in R. solani AG2-2, namely IIIB, IV and LP.
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  • 46
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Rhizoctonia isolates obtained from maize grown in commercial fields in 33 representative counties (or cities) in Sichuan province in China were characterized according to colony morphology, hyphal anastomosis and pathogenicity. Of 141 isolates, 116 were identified as R. solani, 23 as R. zeae and two as binucleate Rhizoctonia. The isolates of R. solani were assigned to four anastomosis groups (AG): AG-1-IA (101 isolates, accounting for 71.6% of the total), AG-1-IB (2, 1.4%), AG-4 (9, 6.4%) and AG-5 (4, 2.8%). The two isolates of binucleate Rhizoctonia belonged to AG-K. On maize, isolates of AG-1-IA caused typical sheath blight symptoms. Lesions produced by isolates of AG-4, AG-5, AG-1-IB and AG-K were darker than those of AG-1-IA. Rhizoctonia zeae usually caused discontinuous lesions with a dark brown margin and a brown centre on the leaf sheaths, as well as ear rot. Isolates of AG-1-IA were the most virulent to maize, with an average lesion length of approximately 15 cm. Isolates of R. zeae produced lesions approximately 12 cm long, while those of AG-4, AG-5, AG-1-IB and AG-K were progressively shorter. On potato dextrose agar (PDA; pH 6.4), the minimum temperature for mycelial growth of R. zeae isolates was 14–18°C, the maximum 38–40°C and optimum 30°C. Isolates of R. zeae did not grow on PDA (28°C) at pH 2.0, the optimum for growth being pH 6.4.
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  • 47
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Publishers
    Plant pathology 47 (1998), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Didymella rabiei grew saprophytically on pieces of artificially and naturally infected chickpea stem debris under artificial incubation conditions, and formed pseudothecia and pycnidia. The extent of growth was not significantly affected by temperature of incubation within the range 5–25°C, but was significantly reduced as relative humidity (RH) decreased from 100% to 86%, when no growth occurred. Pseudothecia matured at 10°C and constant 100% RH, or at 5 and 10°C and alternating 100%/34% RH. Under these conditions, pseudothecial maturation, assessed by a pseudothecia maturity index, increased over time according to the logistic model. For temperatures higher than 10°C or RH lower than 100%, pseudothecia either did not form ascospores, or ascopores did not mature and their content degenerated. When pseudothecia that initially developed to a given developmental stage were further incubated at a constant 100% RH, temperature became less limiting for complete pseudothecial development as the developmental stage was more advanced. Pycnidia of the fungus developed and formed viable conidia in all environmental conditions studied, except at 86% RH. However, the density of pycnidia formed and the number of viable conidia per pycnidium were significantly influenced by temperature, RH and the type of debris (artificially or naturally infected) used.
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    Quarterly review of the rural economy. 23:1 (1970:Jan.) 1 
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    Quarterly review of the rural economy. 23:1 (1970:Jan.) 19 
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    Quarterly review of the rural economy. 23:1 (1970:Jan.) 50 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
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    Quarterly review of the rural economy. 23:1 (1970:Jan.) 35 
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    Quarterly review of the rural economy. 23:1 (1970:Jan.) 51 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
    Description / Table of Contents: Selected Annual Rural Statistics: Australia
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    Quarterly review of the rural economy. 23:1 (1970:Jan.) 52 
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    Description / Table of Contents: Selected Annual Rural Statistics: Australia
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    Quarterly review of the rural economy. 23:1 (1970:Jan.) 53 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
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    Quarterly review of the rural economy. 23:2 (1970:Apr.) 55 
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    Quarterly review of the rural economy. 23:2 (1970:Apr.) 110 
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    Quarterly review of the rural economy. 23:2 (1970:Apr.) 114 
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    Quarterly review of the rural economy. 23:2 (1970:Apr.) 117 
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    Quarterly review of the rural economy. 23:3 (1970:July) 119 
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    Quarterly review of the rural economy. 23:3 (1970:July) 122 
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    Quarterly review of the rural economy. 23:3 (1970:July) 164 
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    Quarterly review of the rural economy. 23:3 (1970:July) 192 
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    Quarterly review of the rural economy. 23:3 (1970:July) 207 
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    Quarterly review of the rural economy. 23:4 (1970:Oct.) 209 
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    Quarterly review of the rural economy. 23:4 (1970:Oct.) 222 
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    Quarterly review of the rural economy. 23:4 (1970:Oct.) 243 
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    Quarterly review of the rural economy. 24:1 (1971:Jan.) 1 
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    Canberra : Periodicals Archive Online (PAO)
    Quarterly review of the rural economy. 24:1 (1971:Jan.) 45 
    ISSN: 0156-7446
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
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    Canberra : Periodicals Archive Online (PAO)
    Quarterly review of the rural economy. 24:1 (1971:Jan.) 57 
    ISSN: 0156-7446
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
    Notes: STATISTICS
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  • 100
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    Canberra : Periodicals Archive Online (PAO)
    Quarterly review of the rural economy. 24:1 (1971:Jan.) 58 
    ISSN: 0156-7446
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Economics
    Description / Table of Contents: Selected Annual Rural Statistics: Australia
    Notes: STATISTICS
    Location Call Number Expected Availability
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