ALBERT

Description: Microcapsules prepared by encapsulating an aqueous solution of a protein, drug or other bioactive substance inside a semi-permeable membrane by are disclosed. The microcapsules are formed by interfacial coacervation under conditions where the shear forces are limited to 0-100 dynes/cm.sup.2 at the interface. By placing the microcapsules in a high osmotic dewatering solution, the protein solution is gradually made saturated and then supersaturated, and the controlled nucleation and crystallization of the protein is achieved. The crystal-filled microcapsules prepared by this method can be conveniently harvested and stored while keeping the encapsulated crystals in essentially pristine condition due to the rugged, protective membrane. Because the membrane components themselves are x-ray transparent, large crystal-containing microcapsules can be individually selected, mounted in x-ray capillary tubes and subjected to high energy x-ray diffraction studies to determine the 3-D structure of the protein molecules. Certain embodiments of the microcapsules of the invention have composite polymeric outer membranes which are somewhat elastic, water insoluble, permeable only to water, salts, and low molecular weight molecules and are structurally stable in fluid shear forces typically encountered in the human vascular system.

Description: The present invention provides a method for production of functional proteins including hormones by renal cells in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel. Natural mixture of renal cells expresses the enzyme 1-a-hydroxylase which can be used to generate the active form of vitamin D: 1,25-diOH vitamin D3. The fibroblast cultures and co-culture of renal cortical cells express the gene for erythropoietin and secrete erythropoietin into the culture supernatant. Other shear stress response genes are also modulated by shear stress, such as toxin receptors megalin and cubulin (gp280). Also provided is a method of treating in-need individual with the functional proteins produced in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel.

Description: A method, simulation, and apparatus are provided that are highly suitable for treatment of benign prostatic hyperplasia (BPH). A catheter is disclosed that includes a small diameter disk loaded monopole antenna surrounded by fusion material having a high heat of fusion and a melting point preferably at or near body temperature. Microwaves from the antenna heat prostatic tissue to promote necrosing of the prostatic tissue that relieves the pressure of the prostatic tissue against the urethra as the body reabsorbs the necrosed or dead tissue. The fusion material keeps the urethra cool by means of the heat of fusion of the fusion material. This prevents damage to the urethra while the prostatic tissue is necrosed. A computer simulation is provided that can be used to predict the resulting temperature profile produced in the prostatic tissue. By changing the various control features of the catheter and method of applying microwave energy a temperature profile can be predicted and produced that is similar to the temperature profile desired for the particular patient.

Description: The present invention relates to telemetry-based sensing systems that continuously measures physical, chemical and biological parameters. More specifically, these sensing systems comprise a small, modular, low-power implantable biotelemetry system capable of continuously sensing physiological characteristics using implantable transmitters, a receiver, and a data acquisition system to analyze and record the transmitted signal over several months. The preferred embodiment is a preterm labor and fetal monitoring system. Key features of the invention include Pulse Interval Modulation (PIM) that is used to send temperature and pressure information out of the biological environment. The RF carrier frequency is 174-216 MHz and a pair of RF bursts (pulses) is transmitted at a frequency of about 1-2 Hz. The transmission range is 3 to 10 feet, depending on the position of the transmitter in the body and its biological environment. The entire transmitter is encapsulated in biocompatible silicone rubber. Power is supplied by on-board silver-oxide batteries. The average power consumption of the current design is less than 30 .mu.W., which yields a lifetime of approximately 6-9 months. Chip-on-Board technology (COB) drastically reduces the size of the printed circuit board from 38.times.28 mm to 22.times.8 mm. Unpackaged dies are flip-chip bonded directly onto the printed circuit board, along with surface mount resistors and capacitors. The invention can monitor additional physiological parameters including, but not limited to, ECG, blood gases, glucose, and ions such as calcium, potassium, and sodium.

Description: A method is presented for determining diastolic intracranial pressure (ICP) in a patient. A first change in the length of a path across the skull of the patient caused by a known change in ICP is measured and used to determine an elasticity constant for the patient. Next, a second change in the length of the path across the patient's skull occurring between systolic and diastolic portions of the patient's heartbeat is measured. The patient's diastolic ICP is a function of the elasticity constant and the second change.

Description: A rotary pump for pumping fluids through a patient having a housing with an internal region, a stator member and an impeller positioned within the housing and having impeller blades, wherein the impeller is magnetically suspended and rotated, and wherein the geometric configuration of the rotary pump is sized and proportioned to minimize stagnant and traumatic fluid flow within the rotary pump. The plurality of magnetic impeller blades are preferably rare earth, high-energy-density magnets selected from the group consisting of samarium cobalt and neodymium-iron-boron alloy.

Description: The vision document provides an overview of the Climate Change Science Program (CCSP) long-term strategic plan to enhance scientific understanding of global climate change.This document is a companion to the comprehensive Strategic Plan for the Climate Change Science Program. The report responds to the Presidents direction that climate change research activities be accelerated to provide the best possible scientific information to support public discussion and decisionmaking on climate-related issues.The plan also responds to Section 104 of the Global Change Research Act of 1990, which mandates the development and periodic updating of a long-term national global change research plan coordinated through the National Science and Technology Council.This is the first comprehensive update of a strategic plan for U.S. global change and climate change research since the origal plan for the U.S. Global Change Research Program was adopted at the inception of the program in 1989.

Description: The purpose of this Science and Implementation Plan is to describe GAPP science objectives and the activities required to meet these objectives, both specifically for the near-term and more generally for the longer-term. The GEWEX Americas Prediction Project (GAPP) is part of the Global Energy and Water Cycle Experiment (GEWEX) initiative that is aimed at observing, understanding and modeling the hydrological cycle and energy fluxes at various time and spatial scales. The mission of GAPP is to demonstrate skill in predicting changes in water resources over intraseasonal-to-interannual time scales, as an integral part of the climate system.

Description: Dr. France Cordova, NASA's Chief Scientist, opened this, the sixth seminar in the Administrator's Seminar Series, by introducing NASA Administrator Daniel S. Goldin. Mr Goldin welcomed the attendees and set the stage for Dr. Cordova's introduction of the first speaker, Dr. Robert Kates of Brown University. Dr. Kates primary concerns are global environmental changes, world hunger, and the size of the population. Human changes, he said, rival the changes of nature. Changes in the size of world population affect the need for more agricultural products, therefore more land for growing food, which leads to deforestation, which affects rainfall, and therefore the water supply which is in increased demand. Human ingenuity can reduce some shortages but generally doesn't keep up with increased demand for life-sustaining essentials. These problems require the concern of intergovernmental organizations, treaties and activities, as well as transnational corporations, and non-governmental and private, volunteer organizations. Next Dr. Diana Liverman of Pennsylvania State University spoke on human interactions regarding climate and society. She considered the effect of changes in land use on climate, using Mexico as an example. Mexicans changed from raising much wheat to raising more fruits and vegetables. This was in response to the demands of the market. The results were more industry, population growth, greater income, drought (because the new crops required more water), and conflicts over water supplies. Dr. Charles Kennel of the Office of Mission to Planet Earth joined Dr.s Cordova, Kates, and Liverman for the question and answer session that followed.

Description: This final rule adopts with changes the interim rule published in the Federal Register on April 5, 2001 (65 FR 18051-18053), which amended the NASA FAR Supplement to implement a Safety and Health (Short Form) clause to address safety and occupational health in all NASA contracts above the micro-purchase threshold where the existing Safety and Health clause did not apply, and amended other safety and health clauses to be consistent with the new NASA Safety and Health (Short Form) clause.

Description: It is hypothesized that the hematopoietic stem cell therapy (HSCT) might countermeasure various space-caused disorders so as to maintain astronauts' homeostasis. If this were achievable, the HSCT could promote human exploration of deep space. Using animal models of disorders (hindlimb suspension unloading system and beta-thalassemia), the HSCT was tested for muscle loss, immunodeficiency and space anemia. The results indicate feasibility of HSCT for these disorders. To facilitate the HSCT in space, growth of HSCs were optimized in the NASA Rotating Wall Vessel (RWV) culture systems, including Hydrodynamic Focusing Bioreactor (HFB).

Description: This document describes some of the life sciences research that was conducted on Spacelab missions. Dr. Larry Young, Director of the National Space Biomedical Research Institute, provides an overview of the Life Sciences Spacelabs.

Description: Much of NASA's Arctic Research is run through its Cryospheric Sciences Program. Arctic research efforts to date have focused primarily on investigations of the mass balance of the largest Arctic land-ice masses and the mechanisms that control it, interactions among sea ice, polar oceans, and the polar atmosphere, atmospheric processes in the polar regions, energy exchanges in the Arctic. All of these efforts have been focused on characterizing, understanding, and predicting, changes in the Arctic. NASA's unique vantage from space provides an important perspective for the study of these large scale processes, while detailed process information is obtained through targeted in situ field and airborne campaigns and models. An overview of NASA investigations in the Arctic will be presented demonstrating how the synthesis of space-based technology, and these complementary components have advanced our understanding of physical processes in the Arctic.

Description: The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

Description: A three-dimensional (3-D), tissue-like model was developed for the genotoxic assessment of space environment. In previous experiments, we found that culturing mammalian cells in a NASA-designed bioreactor, using Cytodex-3 beads as a scaffold, generated 3-D multicellular spheroids. In an effort to generate scaffold-free spheroids, we developed a new 3-D tissue-like model by coculturing fibroblast and epithelial cell in a NASA bioreactor using macroporous Cultispher-S(TradeMark) microcarriers. Big Blue(Registered Trademark) Rat 2(Lambda) fibroblasts, genetically engineered to contain multiple copies (〉60 copies/cell) of the Lac I target gene, were cocultured with radio-sensitive human epithelial cells, H184F5. Over an 8-day period, samples were periodically examined by microscopy and histology to confirm cell attachment, growth, and viability. Immunohistochemistry and western analysis were used to evaluate the expression of specific cytoskeletal and adhesion proteins. Key cell culture parameters (glucose, pH, and lactate concentrations) were monitored daily. Controls were two-dimensional mono layers of fibroblast or epithelial cells cultured in T-flasks. Analysis of 3-D spheroids from the bioreactor suggests fibroblast cells attached to and completely covered the bead surface and inner channels by day 3 in the bioreactor. Treatment of the 3-day spheroids with dispase II dissolved the Cultisphers(TradeMark) and produced multicellular, bead-less constructs. Immunohistochemistry confirmed the presence of vi.mentin, cytokeratin and E-cadherin in treated spheroids. Examination of the dispase II treated spheroids with transmission electron microscopy (TEM) also showed the presence of desmosomes. These results suggest that the controlled enzymatic degradation of an artificial matrix in the low shear environment of the NASA-designed bioreactor can produce 3-D tissue-like spheroids. 2

Description: The present invention provides transgenic plants over-expressing a transgene encoding a calcium-binding protein or peptide (CaBP). Preferably, the CaBP is a calcium storage protein and over-expression thereof does not have undue adverse effects on calcium homeostasis or biochemical pathways that are regulated by calcium. In preferred embodiments, the CaBP is calreticulin (CRT) or calsequestrin. In more preferred embodiments, the CaBP is the C-domain of CRT, a fragment of the C-domain, or multimers of the foregoing. In other preferred embodiments, the CaBP is localized to the endoplasmic reticulum by operatively associating the transgene encoding the CaBP with an endoplasmic reticulum localization peptide. Alternatively, the CaBP is targeted to any other sub-cellular compartment that permits the calcium to be stored in a form that is biologically available to the plant. Also provided are methods of producing plants with desirable phenotypic traits by transformation of the plant with a transgene encoding a CaBP. Such phenotypic traits include increased calcium storage, enhanced resistance to calcium-limiting conditions, enhanced growth and viability, increased disease and stress resistance, enhanced flower and fruit production, reduced senescence, and a decreased need for fertilizer production. Further provided are plants with enhanced nutritional value as human food or animal feed.

Description: NASA conducted the Cirrus Regional Study of Tropical Anvils and Cirrus Layers (CRYSTAL) Florida Area Cirrus Experiment (FACE) during July 2002 for improved understanding of tropical cirrus. One of the goals was to improve the understanding of cirrus generation by convective updrafts. The reasons why some convective storms produce extensive cirrus anvils is only partially related to convective instability and the vertical transport ice mass by updrafts. Convective microphysics must also have an important role on cirrus generation, for example, there are hypotheses that homogeneous nucleation in convective updrafts is a major source of anvil ice particles. In this paper, we report on one intense CRYSTAL- FACE convective case on 16 July 2002 that produced extensive anvil.

Description: The capability for massively parallel programming (MPP) using a message passing interface (MPI) has been implemented into a three-dimensional version of the Goddard Cumulus Ensemble (GCE) model. The design for the MPP with MPI uses the concept of maintaining similar code structure between the whole domain as well as the portions after decomposition. Hence the model follows the same integration for single and multiple tasks (CPUs). Also, it provides for minimal changes to the original code, so it is easily modified and/or managed by the model developers and users who have little knowledge of MPP. The entire model domain could be sliced into one- or two-dimensional decomposition with a halo regime, which is overlaid on partial domains. The halo regime requires that no data be fetched across tasks during the computational stage, but it must be updated before the next computational stage through data exchange via MPI. For reproducible purposes, transposing data among tasks is required for spectral transform (Fast Fourier Transform, FFT), which is used in the anelastic version of the model for solving the pressure equation. The performance of the MPI-implemented codes (i.e., the compressible and anelastic versions) was tested on three different computing platforms. The major results are: 1) both versions have speedups of about 99% up to 256 tasks but not for 512 tasks; 2) the anelastic version has better speedup and efficiency because it requires more computations than that of the compressible version; 3) equal or approximately-equal numbers of slices between the x- and y- directions provide the fastest integration due to fewer data exchanges; and 4) one-dimensional slices in the x-direction result in the slowest integration due to the need for more memory relocation for computation.

Description: A detailed analysis of available in situ and remotely sensed N2O and CH4 data measured in the 1999/2000 winter Arctic vortex has been performed in order to quantify the temporal evolution of vortex descent. Differences in potential temperature (theta) among balloon and aircraft vertical profiles (an average of 19-23 K on a given N2O or CH4 isopleth) indicated significant vortex inhomogeneity in late fall as compared with late winter profiles. A composite fall vortex profile was constructed for 26 November 1999, whose error bars encompassed the observed variability. High-latitude extravortex profiles measured in different years and seasons revealed substantial variability in N2O and CH4 on theta surfaces, but all were clearly distinguishable from the first vortex profiles measured in late fall 1999. From these extravortex-vortex differences we inferred descent prior to 26 November: as much as 397 plus or minus 15 K (lsigma) at 30 ppbv N2O and 640 ppbv CH4, and falling to 28 plus or minus 13 K above 200 ppbv N2O and 1280 ppbv CH4. Changes in theta were determined on five N2O and CH4 isopleths from 26 November through 12 March, and descent rates were calculated on each N2O isopleth for several time intervals. The maximum descent rates were seen between 26 November and 27 January: 0.82 plus or minus 0.20 K/day averaged over 50- 250 ppbv N2O. By late winter (26 February to 12 March), the average rate had decreased to 0.10 plus or minus 0.25 K/day. Descent rates also decreased with increasing N2O; the winter average (26 November to 5 March) descent rate varied from 0.75 plus or minus 0.10 K/day at 50 ppbv to 0.40 plus or minus 0.11 K/day at 250 ppbv. Comparison of these results with observations and models of descent in prior years showed very good overall agreement. Two models of the 1999/2000 vortex descent, SLIMCAT and REPROBUS, despite theta offsets with respect to observed profiles of up to 20 K on most tracer isopleths, produced descent rates that agreed very favorably with the inferred rates from observation.

Description: The ISS (International Space Station) ITCS (Internal Thermal Control System) includes two internal coolant loops that utilize an aqueous based coolant for heat transfer. A silver salt biocide had previously been utilized as an additive in the coolant formulation to control the growth and proliferation of microorganisms within the coolant loops. Ground-based and in-flight testing demonstrated that the silver salt was rapidly depleted, and did not act as an effective long-term biocide. Efforts to select an optimal alternate biocide for the ITCS coolant application have been underway and are now in the final stages. An extensive evaluation of biocides was conducted to down-select to several candidates for test trials and was reported on previously. Criteria for that down-select included: the need for safe, non-intrusive implementation and operation in a functioning system; the ability to control existing planktonic and biofilm residing microorganisms; a negligible impact on system-wetted materials of construction; and a negligible reactivity with existing coolant additives. Candidate testing to provide data for the selection of an optimal alternate biocide is now in the final stages. That testing has included rapid biocide effectiveness screening using Biolog MT2 plates to determine minimum inhibitory concentration (amount that will inhibit visible growth of microorganisms), time kill studies to determine the exposure time required to completely eliminate organism growth, materials compatibility exposure evaluations, coolant compatibility studies, and bench-top simulated coolant testing. This paper reports the current status of the effort to select an alternate biocide for the ISS ITCS coolant. The results of various test results to select the optimal candidate are presented.

Description: The Goddard Cumulus Ensemble (GCE) model is used to examine the sensitivities of surface fluxes, explicit radiation, and ice microphysical processes on multi-day simulations of deep tropical convection over the Tropical Ocean Global Atmosphere Coupled Ocean-Atmosphere Response Experiment (TOGA COARE). The simulations incorporate large-scale advective temperature and moisture forcing, as well as large-scale momentum, that are updated every time step on a periodic lateral boundary grid. This study shows that when surface fluxes are eliminated, the mean atmosphere is much cooler and drier, convection and CAPE are much weaker, precipitation is less, and cloud coverage in stratiform regions much greater. Surface fluxes using the TOGA COARE flux algorithm are weaker than with the aerodynamic formulation, but closer to the observed fluxes. In addition, similar trends noted above for the case without surface fluxes are produced for the TOGA flux case, albeit to a much lesser extent. The elimination of explicit shortwave and longwave radiation is found to have only minimal effects on the mean thermodynamics, convection, and precipitation. However explicit radiation does have a significant impact on cloud temperatures and structure above 200 mb and on the overall mean vertical circulation. The removal of ice processes produces major changes in the structure of the cloud. Much of the liquid water is transported aloft and into anvils above the melting layer (600 mb), leaving narrow, but intense bands of rainfall in convective regions. The elimination of melting processes leads to greater hydrometeor mass below the melting layer, and produces a much warmer and moister boundary layer, leading to a greater mean CAPE. Finally, the elimination of the graupel species has only a small impact on mean total precipitation, thermodynamics, and dynamics of the simulation, but does produce much greater snow mass just above the melting layer. Some of these results differ from previous CRM studies of tropical systems, which is likely due to the type of simulated system, total time integration, and model setup.

Description: Observing system simulation experiments (OSSE's) provide an effective means to evaluate the potential impact of a proposed observing system, as well as to determine tradeoffs in their design, and to evaluate data assimilation methodology. Great care must be taken to ensure realism of the OSSE's, and in the interpretation of OSSE results. All of the OSSE's that have been conducted to date have demonstrated tremendous potential for space-based wind profile data to improve atmospheric analyses, forecasts, and research. This has been true for differing data assimilation systems, analysis methodology, and model resolutions. OSSE's clearly show much greater potential for observations of the complete wind profile than for single-level wind data or observations of the boundary layer alone.

Description: The idea behind genetic algorithms is to extract optimization strategies nature uses successfully - known as Darwinian Evolution - and transform them for application in mathematical optimization theory to find the global optimum in a defined phase space. One could imagine a population of individual 'explorers' sent into the optimization phase-space. Each explorer is defined by its genes, what means, its position inside the phase-space is coded in his genes. Every explorer has the duty to find a value of the quality of his position in the phase space. (Consider the phase-space being a number of variables in some technological process, the value of quality of any position in the phase space - in other words: any set of the variables - can be expressed by the yield of the desired chemical product.) Then the struggle of 'life' begins. The three fundamental principles are selection, mating/crossover, and mutation. Only explorers (= genes) sitting on the best places will reproduce and create a new population. This is performed in the second step (mating/crossover). The 'hope' behind this part of the algorithm is, that 'good' sections of two parents will be recombined to yet better fitting children. In fact, many of the created children will not be successful (as in biological evolution), but a few children will indeed fulfill this hope. These good sections are named in some publications as building blocks. Now there appears a problem. Repeating these steps, no new area would be explored. The two former steps would only exploit the already known regions in the phase space, which could lead to premature convergence of the algorithm with the consequence of missing the global optimum by exploiting some local optimum. The third step, mutation, ensures the necessary accidental effects. One can imagine the new population being mixed up a little bit to bring some new information into this set of genes. Whereas in biology a gene is described as a macro-molecule with four different bases to code the genetic information, a gene in genetic algorithms is usually defined as a bitstring (a sequence of b 1's and 0's).

Description: Spaceborne scatterometers are active microwave radar instruments designed to acquire near-simultaneous, spatially collocated measurements of the normalized radar backscattering cross section (sigma0) of the global surface from several azimuth and/or incidence angles. The primary objective of the scatterometer mission is to measure the near-surface wind speed and direction over the global ocean using sigma0 measurements together with a wind geophysical model function. However, since sigma0 measurements are collected globally all the time, sigma0 data can also be used for global land and ice applications. In this paper, we will first present the objectives of the QSCAT mission, the instrument design, and the unique features of the Ku-band scatterometer currently in operation, called SeaWinds on QuikSCAT (QSCAT). We will then present some emerging land and ocean applications of the QSCAT data, which include (1) global snow detection and monitoring, (2) melt region mapping on the Greenland ice sheet, (3) Monsoon flood detection and monitoring, (4) soil wetness application at large scale, and (5) hurricane monitoring and tracking.

Description: This paper studies the performance of a spaceborne precipitation radar in measuring vertical Doppler velocity of rainfall. As far as a downward pointing precipitation radar is concerned, one of the major problems affecting Doppler measurement at the nadir direction arises from the Non-Uniform Beam-Filling effect (NUBF). That is, when significant variation in rain rate is present within the radar IFOV (Instrument Field of View) in the along track direction. the Doppler shift caused by the radial component of the horizontal speed of the satellite is weighted differently among the portions of IFOV. The effects of this non-uniform weighting may dominate any other contribution. Under this condition, shape, average value and width of the Doppler spectrum may not be directly correlated with the vertical velocity of the precipitating particles. However, by using an inversion technique which over-samples the radar measurements in the along track direction, we show that the shift due to NUBF can be evaluated, and that the NUBF induced errors on average fall speed can be reduced.

Description: The conversion of cloud ice to snow by depositional growth, designated P(sub SFI), in the Goddard Cumulus Ensemble Model cloud physics parameterization is examined. The original formulation of P(sub SFI) is shown to produce excessive conversion of cloud ice to snow because of an implicit assumption that the relative humidity is 100% with respect to water even though the air may actually be quite less humid. Two possible corrections to this problem are proposed, the first involving application of a relative humidity dependent correction factor to the original formulation of P(sub SFI), and the second involving a new formulation of P(sub SFI) based on the equation for depositional growth of cloud ice.

Description: This project, supported by the NASA New Investigator Program, has primarily been funding a graduate student, Darren McKague. Since August 1999 Darren has been working part time at Raytheon, while continuing his PhD research. Darren is planning to finish his thesis work in May 2001, thus some of the work described here is ongoing. The proposed research was to use GOES visible and infrared imager data and SSM/I microwave data to obtain joint distributions of cirrus cloud ice mass and precipitation for a study region in the Eastern Tropical Pacific. These joint distributions of cirrus cloud and rainfall were to be compared to those from the CSU general circulation model to evaluate the cloud microphysical amd cumulus parameterizations in the GCM. Existing algorithms were to be used for the retrieval of cloud ice water path from GOES (Minnis) and rainfall from SSM/I (Wilheit). A theoretical study using radiative transfer models and realistic variations in cloud and precipitation profiles was to be used to estimate the retrieval errors. Due to the unavailability of the GOES satellite cloud retrieval algorithm from Dr. Minnis (a co-PI), there was a change in the approach and emphasis of the project. The new approach was to develop a completely new type of remote sensing algorithm - one to directly retrieve joint probability density functions (pdf's) of cloud properties from multi-dimensional histograms of satellite radiances. The usual approach is to retrieve individual pixels of variables (i.e. cloud optical depth), and then aggregate the information. Only statistical information is actually needed, however, and so a more direct method is desirable. We developed forward radiative transfer models for the SSM/I and GOES channels, originally for testing the retrieval algorithms. The visible and near infrared ice scattering information is obtained from geometric ray tracing of fractal ice crystals (Andreas Macke), while the mid-infrared and microwave scattering is computed with Mie scattering. The radiative transfer is performed with the Spherical Harmonic Discrete Ordinate Method (developed by the PI), and infrared molecular absorption is included with the correlated k-distribution method. The SHDOM radiances have been validated by comparison to version 2 of DISORT (the community "standard" discrete-ordinates radiative transfer model), however we use SHDOM since it is computationally more efficient.

Description: The microphysical parameterization of clouds and rain-cells plays a central role in atmospheric forward radiative transfer models used in calculating passive microwave brightness temperatures. The absorption and scattering properties of a hydrometeor-laden atmosphere are governed by particle phase, size distribution, aggregate density., shape, and dielectric constant. This study identifies the sensitivity of brightness temperatures with respect to the microphysical cloud parameterization. Cloud parameterizations for wideband (6-410 GHz observations of baseline brightness temperatures were studied for four evolutionary stages of an oceanic convective storm using a five-phase hydrometeor model in a planar-stratified scattering-based radiative transfer model. Five other microphysical cloud parameterizations were compared to the baseline calculations to evaluate brightness temperature sensitivity to gross changes in the hydrometeor size distributions and the ice-air-water ratios in the frozen or partly frozen phase. The comparison shows that, enlarging the rain drop size or adding water to the partly Frozen hydrometeor mix warms brightness temperatures by up to .55 K at 6 GHz. The cooling signature caused by ice scattering intensifies with increasing ice concentrations and at higher frequencies. An additional comparison to measured Convection and Moisture LA Experiment (CAMEX 3) brightness temperatures shows that in general all but, two parameterizations produce calculated T(sub B)'s that fall within the observed clear-air minima and maxima. The exceptions are for parameterizations that, enhance the scattering characteristics of frozen hydrometeors.

Description: A persistent, mesoscale region of intense eyewall convection contained within Hurricane Bonnie on 23 August 1998 is examined from multiple observations synthesized from the NASA ER-2 and DC-8 aircraft. The intense convection occurred late in the day as Bonnie was attaining its minimum central pressure and during a stage when the inner core featured a markedly asymmetric structure. The main purpose of this paper is to describe the internal structure of a convective burst and its relationship to the warm core using a synthesis of high-resolution satellite, aircraft radar, and in situ data. An exceptionally vigorous eyewall tower penetrating to nearly 18 km is described. A second intense eyewall tower, adjacent to the eye, is shown to be associated with a mesoscale subsiding current of air that extends horizontally nearly 20 km into the eye interior. The subsidence occupies a substantial depth within the eye and appears to be a much larger scale feature than the convectively-induced, symmetric overturning which commonly occurs on the upper-level flanks of convective towers in other tropical environments.

Description: Ensemble sets of simulation experiments were conducted with a single column model (SCM) using the Goddard GEOS II GCM physics containing a recent version of the Cumulus Scheme (McRAS) and a biosphere based land-fluxes scheme (SSiB). The study used the 18 July to 5 August 1995 ARM-CART (Atmospheric Radiation Measurement-Cloud Atmospheric Radiation Test-bed) data, which was collected at the ARM-CART site in the mid-western United States and analyzed for single column modeling (SCM) studies. The new findings affirm the earlier findings that the vegetation, which increases the solar energy absorption at the surface together with soil and soil-moisture dependent processes, which modulate the surface, fluxes (particularly evapotranspiration) together help to increase the local rainfall. In addition, the results also show that for the particular study period roughly 50% of the increased evaporation over the ARM-CART site would be converted into rainfall with the Column, while the remainder would be advected out to the large-scale. Notwithstanding the limitations of only one-way interaction (i.e., the large-scale influencing the regional physics and not vice versa), the current SCM simulations show a very robust relationship. The evaporation-precipitation relationship turns out to be independent of the soil types, and soil moisture; however, it is weakly dependent on the vegetation cover because of its surface-albedo effect. Clearly, these inferences are prone to weaknesses of the SCM physics, the assumptions of the large-scale being unaffected by gridscale (SCM-scale) changes in moist processes, and other limitations of the evaluation procedures.

Description: This chapter will very briefly introduce and review some computational experiments in using trainable gene regulation network models to simulate and understand selected episodes in the development of the fruit fly, Drosophila melanogaster. For details the reader is referred to the papers introduced below. It will then introduce a new gene regulation network model which can describe promoter-level substructure in gene regulation. As described in chapter 2, gene regulation may be thought of as a combination of cis-acting regulation by the extended promoter of a gene (including all regulatory sequences) by way of the transcription complex, and of trans-acting regulation by the transcription factor products of other genes. If we simplify the cis-action by using a phenomenological model which can be tuned to data, such as a unit or other small portion of an artificial neural network, then the full transacting interaction between multiple genes during development can be modelled as a larger network which can again be tuned or trained to data. The larger network will in general need to have recurrent (feedback) connections since at least some real gene regulation networks do. This is the basic modeling approach taken, which describes how a set of recurrent neural networks can be used as a modeling language for multiple developmental processes including gene regulation within a single cell, cell-cell communication, and cell division. Such network models have been called "gene circuits", "gene regulation networks", or "genetic regulatory networks", sometimes without distinguishing the models from the actual modeled systems.

Description: Nucleozymes containing ribonucleotides and deoxyribonucleotides or nucleic acid analogues are described herein. The nucleozymes have catalytic activity and are significantly more resistant to degradation than their all-RNA ribozyme counterparts. Also described are methods for preparing the nucleozymes along with methods of using nucleozymes, e.g., as therapeutic agents.

Description: Encrusting bryozoans provide one of the few systems in the fossil record in which ecological competition can be observed directly at local scales. The macroevolutionary history of diversity of cyclostome and cheilostome bryozoans is consistent with a coupled-logistic model of clade displacement predicated on species within clades interacting competitively. The model matches observed diversity history if the model is perturbed by a mass extinction with a position and magnitude analogous to the Cretaceous/Tertiary boundary event, Although it is difficult to measure all parameters in the model from fossil data, critical factors are intrinsic rates of extinction, which can be measured. Cyclostomes maintained a rather low rate of extinction, and the model solutions predict that they would lose diversity only slowly as competitively superior species of cheilostomes diversified into their environment. Thus, the microecological record of preserved competitive interactions between cyclostome and cheilostome bryozoans and the macroevolutionary record of global diversity are consistent in regard to competition as a significant influence on diversity histories of post-Paleozoic bryozoans.

Description: Near infrared spectroscopy is a non-invasive technique that allows determination of tissue oxygenation/blood flow based on spectrophotometric quantitation of oxy- and deoxyhemoglobin present within a tissue. This technique has gained acceptance as a means of detecting and quantifying changes in tissue blood flow due to physiological perturbation, such as that which is elicited in skeletal muscle during exercise. Since the NIRS technique requires light to penetrate the skin and subcutaneous fat in order to reach the muscle of interest, changes in skin blood flow may alter the NIRS signal in a fashion unrelated to blood flow in the muscle of interest. The aim of this study was to determine the contribution of skin blood flow to the NIRS signal obtained from resting vastus lateralis muscle of the thigh.

Description: A response is provided to Diem and colleagues discussion on the whether urbanization-enhanced precipitation should be maximized in the South-Southeast of Atlanta, Georgia as documented in Shepherd et al. (2003). Diem and colleagues have offered a critical response to Shepherd et al. (2002). The response herein offers both general and specific responses to issues raised by Diem and colleagues.

Description: In vitro evolution methods were used to obtain DNA enzymes that cleave either a 2',5' - phosphodiester following a wibonucleotide or a 3',5' -phosphodiester following an L-ribonucleotide. Both enzymes can operate in an intermolecular reaction format with multiple turnover. The DNA enzyme that cleaves a 2',5' -phosphodiester exhibits a k(sub cat) of approx. 0.01/ min and catalytic efficiency, k(sub cat)/k(sub m) of approx. 10(exp 5)/ M min. The enzyme that cleaves an L-ribonudeotide is about 10-fold slower and has a catalytic efficiency of approx. 4 x 10(exp 5)/ M min. Both enzymes require a divalent metal cation for their activity and have optimal catalytic rate at pH 7-8 and 35-50 C. In a comparison of each enzyme s activity with either its corresponding substrate that contains an unnatural ribonudeotide or a substrate that instead contains a standard ribonucleotide, the 2',5' -phosphodiester-deaving DNA enzyme exhibited a regioselectivity of 6000- fold, while the L-ribonucleotide-cleaving DNA enzyme exhibited an enantioselectivity of 50-fold. These molecules demonstrate how in vitro evolution can be used to obtain regio- and enantioselective catalysts that exhibit specificities for nonnatural analogues of biological compounds.

Description: Measurements from the Halogen Occultation Experiment (HALOE) revealed the infrared signature of polar mesospheric clouds (PMCs), for the first time, HALOE PMC observations at eight wavelengths (2.45 - 10 microns) show remarkable agreement with model PMC spectra based on ice particle extinction, and thus provide the first confirmation that water ice is the primary component of PMCs. Because PMCs respond to changes in temperature and water vapor, they are considered an indicator of global climate change. We propose to further the understanding of PMCs using a decade of infrared measurements form HALOE. This effort will characterize PMC spectral properties, extinction profiles, and size distributions. Using this information, HALOE measurements will be used to make simultaneous retrievals of H2O3, and temperature, in the presence of PMCs. The simultaneous retrievals of particle properties, H2O3, and temperature will be used with HALOE NO data to provide a significant step forward in the knowledge of PMC characteristics and formation conditions. We will challenge fundamental theories of PMC formation, and investigate changes in PMC properties and related conditions over the length of the HALOE measurement record. HALOE has been operating without flaw since it was launched on October 11, 1991. Consequently, ten southern and ten northern PMC seasons have been observed thus far, providing a wealth of data for the study of PMC and related parameters.

Description: The primary purpose of this project was to evaluate and improve models for the bulk-skin temperature difference to the point where they could accurately and reliably apply under a wide variety of environmental conditions. To accomplish this goal, work was conducted in three primary areas. These included production of an archive of available data sets containing measurements of the skin and bulk temperatures and associated environmental conditions, evaluation of existing skin layer models using the compiled data archive, and additional theoretical work on the development of an improved model using the data collected under diverse environmental conditions. In this work we set the basis for a new physical model of renewal type, and propose a parameterization for the temperature difference across the cool skin of the ocean in which the effects of thermal buoyancy, wind stress, and microscale breaking are all integrated by means of the appropriate renewal time scales. Ideally, we seek to obtain a model that will accurately apply under a wide variety of environmental conditions. A summary of the work in each of these areas is included in this report. A large amount of work was accomplished under the support of this grant. The grant supported the graduate studies of Sandra Castro and the preparation of her thesis which will be completed later this year. This work led to poster presentations at the 1999 American Geophysical Union Fall Meeting and 2000 IGARSS meeting. Additional work will be presented in a talk at this year's American Meteorological Society Air-Sea Interaction Meeting this May. The grant also supported Sandra Castro during a two week experiment aboard the R/P Flip (led by Dr. Andrew Jessup of the Applied Physics Laboratory) to help obtain additional shared data sets and to provide Sandra with a fundamental understanding of the physical processes needed in the models. In a related area, the funding also partially supported Dr. William Emery and Daniel Baldwin in the preparation of their publication "Accuracy of in situ sea surface temperatures used to calibrate infrared satellite measurements". The remainder of this report is drawn from these publications and presentations.

Description: A laboratory experiment was conducted on cultures of Micromonas pusilla, a marine prasinophyte, to investigate how cell growth and division affect the optical properties over the light:dark cycle. Measurements were made of cell size and concentration, attenuation and absorption coefficients, flow cytometric light scattering (in forward and side directions), chlorophyll and carbon content. Refractive index was calculated using the anomalous diffraction approximation Cells were about 1.5 micrometers in diameter and exhibited phased division, with the major division burst occurring during the night. Typical diel variations were observed, with cells increasing in size and light scattering during the day as they photosynthesize and decreasing at night upon division. The cells were in ultradian growth, with more than one division per day, at a light level of 120 Mu-mol photons m/sq/sec. Since these cells are similar in size to small phytoplankton that are typically abundant in field samples, these results can be used in the interpretation of diel variations in light scattering in natural populations of phytoplankton.

Description: Heavy rainfall occurred over the western side of Taiwan's complex terrain from August 10 to 13, 1994 after Typhoon Doug moved northward from the East China Sea into Taiwan and on towards the Yellow Sea. On August 10, most of the rainfall fell over sloped areas. The heaviest daily rainfall totals were in excess of 200 mm over southwestern as well as central Taiwan. However, not much rainfall occurred over northern Taiwan. The lack of rainfall over northern Taiwan also occurred on August 11, 12 and 13. The larger rainfall amounts shifted westward from the sloped areas on August 10 toward lower terrain on August 11. On August 12 and 13, most of the higher rainfall amounts were found over the coastal area in southwestern Taiwan. Notably, about 300 to 400 mm per day fell over the coastal area in southwest Taiwan on August 12 and 13. The distribution of rainfall amount was different on August 10 and 11 (termed as Case 1) compared to August 12 and 13 (termed as Case 2). The environmental situation and precipitation characteristics are analyzed using EC/TOGA data, ground-based radar data, surface rainfall patterns, surface wind data, and upper air soundings. Chen at al. (2001) also categorized the precipitation pattern into two types, propagating and quasi-stationary. For the propagating type of precipitation, rainrates increased or remained the same as systems went from the plains to mountainous regions. With the quasi-stationary type of precipitation, however, rainrates decreased as precipitation propagated across the plains and into the mountains. The focus of this study is to understand what causes the h1aher amounts of rainfall over Taiwan, and what factors influence where the higher amounts of rainfall will occur, over sloped areas or over coastal areas.

Description: Spaceflight adversely affects the skeleton, posing a substantial risk to astronaut's health during long duration missions. The reduced bone mass observed in growing animals following spaceflight is due at least in part to inadequate bone formation by osteoblasts. Thus, it is of central importance to identify basic cellular mechanisms underlying normal bone formation. The fundamental ideas underlying our research are that interactions between extracellular matrix proteins, integrin adhesion receptors, cytoplasmic signaling and cytoskeletal proteins are key ingredients for the proper functioning of osteoblasts, and that gravity impacts these interactions. As an in vitro model system we used primary fetal rat calvarial cells which faithfully recapitulate osteoblast differentiation characteristically observed in vivo. We showed that specific integrin receptors ((alpha)3(beta)1), ((alpha)5(beta)1), ((alpha)8(betal)1) and extracellular matrix proteins (fibronectin, laminin) were needed for the differentiation of immature osteoblasts. In the course of maturation, cultured osteoblasts switched from depending on fibronectin and laminin for differentiation to depending on these proteins for their very survival. Furthermore, we found that manipulating the gravity vector using ground-based models resulted in activation of key intracellular survival signals generated by integrin/extracellular matrix interactions. We are currently testing the in vivo relevance of some of these observations using targeted transgenic technology. In conclusion, mechanical factors including gravity may participate in regulating survival via cellular interactions with the extracellular matrix. This leads us to speculate that microgravity adversely affects the survival of osteoblasts and contributes to spaceflight-induced osteoporosis.

Description: An observing system comprised of two lidars in geosynchronous orbit would enable the synoptic and meso-scale measurement of atmospheric winds and moisture, both of which are key first-order variables of the Earth's weather equation. Simultaneous measurement of these parameters at fast revisit rates promises large advancements in our weather prediction skills. Such capabilities would be unprecedented and yield greatly improved and finer resolution initial conditions for models, make existing costly and cumbersome measurement approaches obsolete, and obviate the use of numerical techniques needed to correct data obtained using present observing systems. Additionally, simultaneous synoptic wind and moisture observations would lead to improvements in model parameterizations, and in our knowledge of small-scale weather processes. Technology and science data product assessments are ongoing. Results will be presented during the conference.

Description: The development of a satellite infrared technique for estimating convective and stratiform rainfall and its application in studying the diurnal variability of rainfall in Amazonia are presented. The Convective-Stratiform. Technique, calibrated by coincident, physically retrieved rain rates from the Tropical Rain Measuring Mission (TRMM) Microwave Imager (TMI), is applied during January to April 1999 over northern South America. The diurnal cycle of rainfall, as well as the division between convective and stratiform rainfall is presented. Results compare well (a one-hour lag) with the diurnal cycle derived from Tropical Ocean-Global Atmosphere (TOGA) radar-estimated rainfall in Rondonia. The satellite estimates reveal that the convective rain constitutes, in the mean, 24% of the rain area while accounting for 67% of the rain volume. The effects of geography (rivers, lakes, coasts) and topography on the diurnal cycle of convection are examined. In particular, the Amazon River, downstream of Manaus, is shown to both enhance early morning rainfall and inhibit afternoon convection. Monthly estimates from this technique, dubbed CST/TMI, are verified over a dense rain gage network in the state of Ceara, in northeast Brazil. The CST/TMI showed a high bias equal to +33% of the gage mean, indicating that possibly the TMI estimates alone are also high. The root mean square difference (after removal of the bias) equaled 36.6% of the gage mean. The correlation coefficient was 0.77 based on 72 station-months.

Description: Global rainfall is the primary redistributor of earth's energy by the process of latent heat release. This forms the main driving force for the tropical circulation, which in turn impacts the global circulation .through transient events-such as El Nino. Hence, more precise and long-term time series of the rainfall and its variability is crucial to the understanding and prediction of the global climate and climate change. The Precipitation Radar (PR) abroad the US/Japan Tropical Rainfall Measuring Mission (TRMM) is the first radar ever launched into space that measures detailed vertical profiles of rain intensity over the tropics. One of the challenges in estimating rainfall from spaceborne radars is the presence of attenuation at frequencies, such as 14 GHz of the TRMM PR and future planned systems at this and higher frequencies. A common approach in current rainfall retrieval algorithms is to employed the path integrated attenuation (PIA) as a constraint to the retrieval, and hence overcome errors in the radar calibration or in the assumed rainfall parameters. PIA can either be derived from a radiometer or from the surface reference technique, in which a clear air radar measurement is compared with the measurement in the raining area. The current TRMM 2A21 PIA data product makes use of both a temporal and spatial clear air database for comparison to rainy measurements. In this paper we present results from analysis of TRMM surface backscatter cross-section (sigmaO) measurements from Nov 97-Feb99, and a comparison with sigmaO measurements obtained by the NASA Scatterometer (NSCAT) between Sept96-June97. Measurements for a given month from both instruments are compiled on a 1 deg. (lat.) x 1 deg. (lon.) x 1 hr. grid. This enables TRMM--NSCAT comparison and the investigation of seasonal and diurnal trends in both data sets. From preliminary analysis of TRMM sigmaO's we have decided not to treat the ocean as a single homogeneous region but to select a number of ocean sub-regions and individually analyze their trends. Likewise, and in a similar approach to previous studies of Seasat over-land data, we have selected a number of over-land regions for study. From said sigmaO maps and regional trend analysis we investigate possible sources of trends and variability. In addition, we study the effects of TRMM PR sensitivity through the PR "possible rain" class. Given NSCAT's inability to flag rain contaminated measurements we are able to gauge the impact of rain contamination on NSCAT monthly sigmaO maps, using TRMM measurements. The research described in this paper was carried out by the Jet Propulsion Laboratory, California Institute of Technology, under contract with the National Aeronautics and Space Administration (NASA), U.S.A.

Description: The NASA ER-2 and DC-8 aircraft collected remote sensing and in situ data sets from Hurricane Bonnie (23, 24, and 26 August 1998) during the Convection And Moisture Experimental-3 (CAMEX-3). Bonnie was an exceptional case where NASA and NOAA had five aircraft sampling both upper levels and lower altitudes. The ER-2 was instrumented with the ER-2 Doppler XBand radar (EDOP) and several radiometers ranging from visible to lower frequency microwaves. EDOP is a fixed dual-beam radar (nadir and forward-looking beams) which allows computation of both vertical and alongtrack horizontal winds. The hurricane secondary circulation is typically difficult to measure at upper levels due to aircraft altitude limitations and sensitivity of the lower altitude airborne radars. EDOP is in principle, well suited to measure these components of the wind. When ER-2 flies across the approximate center of the hurricane circulation, the along-track winds derived from EDOP, are approximately equal to the hurricane radial flow comprising the secondary circulation. Assuming that the hydrometeor fallspeeds can be approximated, the radial and vertical wind components of the secondary circulation can be measured. Since the hydrometeor motions can be estimated with more confidence in the higher altitude ice regions (i.e., graupel and mixed phase are complicated at lower altitudes), the derived radial and vertical winds have higher accuracy at upper levels. On the other hand, the reflectivities are extremely low at higher altitudes, resulting in fewer Doppler velocity estimates.

Description: Inspired by the immense variety of naturally curious explorers (insects, animals, and birds), their wellintegrated biological sensor-processor suites, efficiently packaged in compact but highly dexterous forms, and their complex, intriguing, cooperative behavior, this paper focuses on "Biomorphic Explorers", their defination/classification, their designs, and presents planetary exploration scenarios based on the designs. Judicious blend of bio-inspired concepts and recent advances in micro-air vehicles, microsensors, microinstruments, MEMS, and microprocessors clearly suggests that the time of small, dedicated, low cost explorers that capture some of the key features of biological systems has arrived. Just as even small insects like ants, termites, honey bees etc working cooperatively in colonies can achieve big tasks, the biomorphic explorers hold the potential for obtaining science in-accessible by current large singular exploration platforms.

Description: The present invention discloses nucleic acid enzymes capable of cleaving single-stranded DNA in a site specific manner.

Description: The onset of the southeast Asian monsoon during 1997 and 1998 was simulated by coupling a mesoscale atmospheric model (MM5) and a detailed, land surface model, PLACE (the Parameterization for Land-Atmosphere-Cloud Exchange). The rainfall results from the simulations were compared with observed satellite data from the TRMM (Tropical Rainfall Measuring Mission) TMI (TRMM Microwave Imager) and GPCP (Global Precipitation Climatology Project). The control simulation with the PLACE land surface model and variable sea surface temperature captured the basic signatures of the monsoon onset processes and associated rainfall statistics. Sensitivity tests indicated that simulations were significantly improved by including the PLACE land surface model. The mechanism by which the land surface processes affect the moisture transport and the convection during the onset of the southeast Asian monsoon were analyzed. The results indicated that land surface processes played an important role in modifying the low-level wind field over two major branches of the circulation: the southwest low-level flow over the Indo-China peninsula and the northern, cold frontal intrusion from southern China. The surface sensible and latent heat fluxes modified the low-level temperature distribution and merit, and therefore the low-level wind due to the thermal wind effect. The more realistic forcing of the sensible and latent heat fluxes from the detailed, land surface model improved the low-level wind simulation and associated moisture transport and convection.

Description: A cloud-resolving model is used to study sensitivities of two different microphysical schemes, one is the bulk type, and the other is an explicit bin scheme, in simulating a mid-latitude squall line case (PRE-STORM, June 10-11, 1985). Simulations using different microphysical schemes are compared with each other and also with the observations. Both the bulk and bin models reproduce the general features during the developing and mature stage of the system. The leading convective zone, the trailing stratiform region, the horizontal wind flow patterns, pressure perturbation associated with the storm dynamics, and the cool pool in front of the system all agree well with the observations. Both the observations and the bulk scheme simulation serve as validations for the newly incorporated bin scheme. However, it is also shown that, the bulk and bin simulations have distinct differences, most notably in the stratiform region. Weak convective cells exist in the stratiform region in the bulk simulation, but not in the bin simulation. These weak convective cells in the stratiform region are remnants of the previous stronger convections at the leading edge of the system. The bin simulation, on the other hand, has a horizontally homogeneous stratiform cloud structure, which agrees better with the observations. Preliminary examinations of the downdraft core strength, the potential temperature perturbation, and the evaporative cooling rate show that the differences between the bulk and bin models are due mainly to the stronger low-level evaporative cooling in convective zone simulated in the bulk model. Further quantitative analysis and sensitivity tests for this case using both the bulk and bin models will be presented in a companion paper.

Description: In traditional applications in soil physics it is convention to scale porous media properties, such as hydraulic conductivity, soil water diffusivity, and capillary head, with the gravitational acceleration. In addition, the Richards equation for water flux in partially saturated porous media also contains a gravity term. With the plans to develop plant habitats in space, such as in the International Space Station, it becomes necessary to evaluate these properties and this equation under conditions of microgravitational acceleration. This article develops models for microgravity steady state two-phase flow, as found in irrigation systems, that addresses critical design issues. Conventional dimensionless groups in two-phase mathematical models are scaled with gravity, which must be assigned a value of zero for microgravity modeling. The use of these conventional solutions in microgravity, therefore, is not possible. This article therefore introduces new dimensionless groups for two-phase models. The microgravity models introduced here determined that in addition to porous media properties, important design factors for microgravity systems include applied water potential and the ratio of inner to outer radii for cylindrical and spherical porous media systems.

Description: Despite the obvious notion that the presence of hail or graupel is a good indication of convection, the model results show this does not provide an objective benchmark partly due to the unrealistic presence of small amounts of hail or graupel throughout the anvil in the model but mainly because of the significant amounts of hail or graupel, especially in the tropical TOGA COARE simulation, in the transition zone. Without use of a "transition" category, it is open to debate as how this region should best be defined, as stratiform or as convective. So, the presence of significant hail or graupel contents in this zone significantly degrades its use an objective benchmark for convection. The separation algorithm comparison was done in the context of a cloud-resolving model. These models are widely used and serve a variety of purposes especially with regard to retrieving information that cannot be directly measured by providing synthetic data sets that are consistent and complete. Separation algorithms are regularly applied in these models. However, as with any modeling system, these types 'of models are constantly being improved to overcome any known deficiencies and make them more accurate representations of observed systems. The presence of hail and graupel in the anvil and the bias towards heavy rainfall rates are two such examples of areas that need improvement. Since, both of these can effect the perceived performance of the separation algorithms, the Lang et al. (2003) study did not want to overstate the relative performance of any specific algorithms.

Description: In this study, we investigate the seasonal variations in surface rainfall and associated large-scale processes in the tropical eastern Atlantic and West African region. The 5-yr (1998-2002) high-quality TRMM rainfall, sea surface temperature (SST), water vapor and cloud liquid water observations are applied along with the NCEP/NCAR reanalysis wind components and a 3-yr (2000-2002) Quickscat satellite-observed surface wind product. Major mean rainfall over West Africa tends to be concentrated in two regions and is observed in two different seasons, manifesting an abrupt shift of the mean rainfall zone during June-July. (i) Near the Gulf of Guinea (about 5 degN), intense convection and rainfall are seen during April-June and roughly follow the seasonality of SST in the tropical eastern Atlantic. (ii) Along the latitudes of about 10 deg. N over the interior West African continent, a second intense rain belt begins to develop from July and remains there during the later summer season. This belt co-exists with a northwardmoved African Easterly Jet (AEJ) and its accompanying horizonal and vertical shear zones, the appearance and intensification of an upper tropospheric Tropical Easterly Jet (TEJ), and a strong low-level westerly flow. Westward-propagating wave signals [ i e . , African easterly waves (AEWs)] dominate the synoptic-scale variability during July-September, in contrast to the evident eastward-propagating wave signals during May- June. The abrupt shift of mean rainfall zone thus turns out to be a combination of two different physical processes: (i) Evident seasonal cycles in the tropical eastern Atlantic ocean which modulate convection and rainfall in the Gulf of Guinea by means of SST thermal forcing and SST-related meridional gradient; (ii) The interaction among the AEJ, TEJ, low-level westerly flow, moist convection and AEWs during July-September which modulates rainfall variability in the interior West Africa, primarily within the ITCZ rain band. Evident seasonality in synoptic-scale wave signals is shown to be a good evidence for this seasonal evolution.

Description: Plant organs may respond to gravity by vertical (orthogravitropic), oblique (plagiogravitropic) or horizontal (diagravitropic) growth. Primary roots of maize (Zea mays L.) provide a good system for studying such behaviours because they are reportedly capable of displaying all three responses. In current work using maize seedlings of the Silver Queen cultivar, stabilisation of growth at an oblique orientation was commonplace. Hypothetically, plagiogravitropism may be accomplished either by a process we call graded orthogravitropism or by hunting about a sensed non-vertical setpoint. In graded orthotropism primary bending is unidirectional and depends on facilitative stimuli that determine its extent. The hallmark of the setpoint mechanism is restorative curvature of either sign following a displacement; both diagravitropism and orthogravitropism are based on setpoints. Roots settled in a plagiogravitropic orientation were tested with various illumination and displacement protocols designed to distinguish between these two hypotheses. The tests refuted the setpoint hypothesis and supported that of graded orthotropism. No evidence of diagravitropism could be found, thus, earlier claims were likely based on inadequately controlled observations of graded orthotropism. We propose that orthotropism is graded by the sequential action of dual gravity receptors: induction of a vectorial gravitropic response requires gravitational induction of a separate facilitative response, whose decay in the absence of fresh stimuli can brake gravitropism at plagiotropic angles.

Description: Gravitropism of vascular plants has been assumed to require a single gravity receptor mechanism. However, based on the evidence in Part I of this study, we propose that maize roots require two. The first mechanism is without a directional effect and, by itself, cannot give rise to tropism. Its role is quantitative facilitation of the second mechanism, which is directional like the gravitational force itself and provides the impetus for tropic curvature. How closely coupled the two mechanisms may be is, as yet, unclear. The evidence for dual receptors supports a general model for roots. When readiness for gravifacilitation, or gravifacilitation itself, is constitutive, orthogravitropic curvature can go to completion. If not constitutively enabled, gravifacilitation can be weak in the absence of light and water deficit or strong in the presence of light and water deficit. In either case, it can decay and permit roots to assume reproducible non-vertical orientations (plagiogravitropic or plagiotropic orientations) without using non-vertical setpoints. In this way roots are deployed in a large volume of soil. Gravitropic behaviours in shoots are more diverse than in roots, utilising oblique and horizontal as well as vertical setpoints. As a guide to future experiments, we assess how constitutive v. non-constitutive modes of gravifacilitation might contribute to behaviours based on each kind of setpoint.

Description: We seek funding from NASA for the third year (2005) of the four-year period January 1, 2003 - December 31, 2006 for continued support of the MIT contributions to the multi-national global atmospheric trace species measurement program entitled Advanced Global Atmospheric Gases Experiment (AGAGE). The case for real-time high-frequency measurement networks like AGAGE is very strong and the observations and their interpretation are widely recognized for their importance to ozone depletion and climate change studies and to verification issues arising from the Montreal Protocol (ozone) and Kyoto Protocol (climate). The proposed AGAGE program is distinguished by its capability to measure over the globe at high frequency almost all of the important species in the Montreal Protocol and almost all of the significant non-CO2 gases in the Kyoto Protocol.

Description: A revolutionary, low-calorie sugar is now available to the food and beverage market, offering an all-natural alternative to table sugar and artificial sweeteners. Tagatose, a sugar that appears in nature in small quantities, began its unusual journey to the commercial market nearly 30 years ago, when Dr. Gilbert V. Levin invented a life detection experiment to place aboard NASA s Mars Viking 1 and Viking 2 landers. The experiment involved using radiation-laced nutrients to determine the presence of microbial life in Martian soil samples.

Description: Computational phenomena (i.e., spurious supersaturation and negative mixing ratio of cloud water) usually exist in cloud-resolving models when the time step for explicit integration is larger than a microphysical timescale in clouds. In this paper, the microphysical timescales in clouds are studied, showing that the timescale of water vapor condensation (or cloud water evaporation) is smaller than 10 s - the order of a typical time step for cloud-resolving models. To avoid spurious computational phenomena in cloud-resolving modeling, it is suggested that moist entropy be used as a prognostic thermodynamic variable, and temperature be diagnosed from that and other prognostic variables. A simple numerical model with moist entropy as a prognostic variable, for example, is presented to show that spurious computational phenomena are removed when moist entropy is used as a prognostic variable.

Description: Recently Grabowski and Khairoutdinov and Randall have proposed and demonstrated, the feasibility of using 2D CRMs (these did not include detailed land processes and used periodic lateral boundary conditions) as 'super-parameterizations or a multi-scale modeling framework MMF' for cloud processes within atmospheric generation circulation models (GCMs). In the MMF, a fine-resolution 2D CRM takes the place of the single-column parameterization that is used in conventional GCMs. An overview of this approach is given by Randall et al.. Since a CRM can explicitly simulate cloud processes at the natural space and time scales of clouddynamical processes, it's possible to compute statistics of cloud fields, including radiances and radar backscatters, directly simulated/produced by the CRM. A seed fund is available at NASA Goddard to build a MMF based on a 2D GCE model and the Goddard fvGCM. A prototype MMF will be developed at end of 2004. The major objectives are to test and evaluate its performance using the GCE model as the super parameterization and compare its results with other super parameterizations, conventional GCMs and observations. Recently, a detailed spectral-bin microphysical scheme was implemented into the Goddard Cumulus Ensemble (WE) model. The GCE model is a CRM and it has been improved and applied to study the precipitation processes in the past two decades. Atmospheric aerosols are also described using number density size-distribution functions. The model is tested by studying the evolution of deep tropical clouds in the west Pacific warm pool region and summertime convection over a mid-latitude continent with different concentrations of CCN: a low 'clean' concentration and a high 'dirty' concentration. The impact of atmospheric aerosol concentration on cloud and precipitation will be investigated. In addition, the similarities and differences between bulk- and spectral-bin microphysics will be presented,

Description: Thin films of an organic nonlinear optical (NLO) material, N, N-dimethyl-p-(2,2-dicyanovinyl) aniline (DCVA), have been grown in space and on the ground by physical vapor transport in an effusive ampoule arrangement. The thin film growth technique developed on the ground is a direct result of information gleaned from experiments in microgravity. This paper covers the results of our experimental investigations for establishing "ideal" terrestrial conditions for deposition of a DCVA film. The active control during the deposition process was exercised by three deposition variables: the material source temperature, the background pressure external to the growth ampoule and the substrate temperature. Successful growth occurred when the difference in temperature between the source material and the copper substrate was 14 degrees C and the background nitrogen pressure was such that the transport was either diffusive or convective. A qualitative diffusion limited boundary was estimated to occur at a pressure of approximately 20 torr. We have probed the DCVA thin films with visible-near infrared reflection absorption spectroscopy, polarized Fourier transform infrared spectrometry, differential interference contrast optical microscopy, and stylus profilometry.

Description: Fast amplitude variations due to atmospheric scintillation are the main concerns for the Deep Space Network (DSN) Ka-band downlink under clear weather conditions. A theoretical study of the amplitude scintillation variances for a finite aperture antenna is presented. Amplitude variances for weak scattering scenarios are examined using turbulence theory to describe atmospheric irregularities. We first apply the Kolmogorov turbulent spectrum to a point receiver for three different turbulent profile models, especially for an exponential model varying with altitude. These analytic solutions then are extended to a receiver with a finite aperture antenna for the three profile models. Smoothing effects of antenna aperture are expressed by gain factors. A group of scaling factor relations is derived to show the dependences of amplitude variances on signal wavelength, antenna size, and elevation angle. Finally, we use these analytic solutions to estimate the scintillation intensity for a DSN Goldstone 34-m receiving station. We find that the (rms) amplitude fluctuation is 0.13 dB at 20-deg elevation angle for an exponential model, while the fluctuation is 0.05 dB at 90 deg. These results will aid us in telecommunication system design and signal-fading prediction. They also provide a theoretical basis for further comparison with other measurements at Ka-band.

Description: Etiolated seedlings frequently display a hypocotyl or epicotyl hook which opens on exposure to light. Etylene has been shown to be necessary for maintenance of the hook in a number of plants in darkness. We investigated the interaction of ethylene and light in the regulation of hypocotyl hook opening in Arabidopsis thaliana. We found that hooks of Arabidopsis open in response to continuous red, far-red or blue light in the presence of up to 100 microliters l-1 ethylene. Thus a change in sensitivity to ethylene is likely to be responsible for hook opening in Arabidopsis, rather than a decrease in ethylene production in hook tissues. We used photomorphogenic mutants of Arabidopsis to demonstrate the involvement of both blue light and phytochrome photosensory systems in light-induced hook opening in the presence of ethylene. In addition we used ethylene mutants and inhibitors of ethylene action to investigate the role of ethylene in hook maintenance in seedlings grown in light and darkness.

Description: Recent events in the International Space Station (ISS) Program have resulted in the necessity to re-examine the research priorities and research plans for future years. Due to both technical and fiscal resource constraints expected on the International Space Station, it is imperative that research priorities be carefully reviewed and clearly articulated. In consultation with OSTP and the Office of Management and budget (OMB), NASA's Office of Biological and Physical Research (OBPR) assembled an ad-hoc external advisory committee, the Biological and Physical Research Maximization and Prioritization (REMAP) Task Force. This paper describes the outcome of the Task Force and how it is being used to define a roadmap for near and long-term Biological and Physical Research objectives that supports NASA's Vision and Mission. Additionally, the paper discusses further prioritizations that were necessitated by budget and ISS resource constraints in order to maximize utilization of the International Space Station. Finally, a process has been developed to integrate the requirements for this prioritized research with other agency requirements to develop an integrated ISS assembly and utilization plan that maximizes scientific output. c2003 American Institute of Aeronautics and Astronautics. Published by Elsevier Science Ltd. All rights reserved.

Description: A key aspect of this project is the establishment of a causal link from circulation anomalies in the lower mesosphere and stratopause region downward through the stratosphere to the troposphere. The observational link for stratospheric sudden warmings and surface climate is fairly clear. However, our understanding of the dynamics is incomplete. We have been making significant progress in the area of dynamical mechanisms by which circulation anomalies in the stratosphere affect the troposphere. We are trying to understand the details and sequence of events that occur when a middle atmosphere (wind) anomaly propagates downward to near the tropopause. The wind anomaly could be caused by a warming or solar variations in the low-latitude stratopause region, or could have other causes. The observations show a picture that is consistent with a circulation anomaly that descends to the tropopause region, and can be detected as low as the mid-troposphere. Processes near the stratopause in the tropics appear to be important precursors to the wintertime development of the northern polar vortex. This may affect significantly our understanding of the process by which low-latitude wind anomalies in the low mesosphere and upper stratosphere evolve through the winter and affect the polar vortex.

Description: The Pennsylvania State University-National Center for Atmospheric Research mesoscale model MM5 is used to simulate Hurricane Bonnie at high resolution (2-km spacing) in order to examine budgets of water vapor, cloud condensate, and precipitation. Virtually all budget terms are derived directly from the model (except for the effects of storm motion). The water vapor budget reveals that a majority of the condensation in the eyewall occurs in convective hot towers, while outside of the eyewall most of the condensation occurs in weak updrafts, indicative of a dominance of stratiform precipitation processes. The ocean source of water vapor in the eyewall region is only a very small fraction of that transported inward in the boundary layer inflow or that condensed in the updrafts. In contrast, in the outer regions, the ocean vapor source is larger owing to the larger area, counters the drying effect of low-level subsidence, and enhances the moisture transported in toward the eyewall. In this mature storm, cloud condensate is consumed as rapidly as it is produced. Cloud water peaks at the top of the boundary layer and within the melting layer, where. cooling from melting enhances condensation. Unlike squall lines, in the hurricane, very little condensate produced in the eyewall convection is transported outward into the surrounding precipitation area. Most of the mass ejected outward is likely in the form of small snow particles that seed the outer regions and enhance stratiform precipitation development through additional growth by vapor deposition and aggregation. In addition, artificial source terms for cloud and precipitation mass associated with setting negative mixing ratios, produced as a result of numerical advection errors, to zero are described. Although small at any given point and time, the cumulative effect of these terms contributes an amount of mass equivalent to 13% of the total condensation. Thus, these terms must be accounted for to balance the model budgets and the results suggest the need for improved model numerics.

Description: The notion that the continental-scale land-sea contrast is the main reason that monsoon circulation exists has been a long-held belief. The purpose of this paper is to point out that this notion should be substantially modified. The central idea of this notion states that in summer, radiative heating of the continent, say Asia, gives rise to a continental-scale thermal low and surrounding the thermal low in its southeast direction the low level wind flows in from south-west. This low-level inflow creates a convergence of moisture, which maintains the cumulus convection. And in winter, radiative cooling of continent gives rise to a thermal high and to its southeast the low-level wind is from northeast. The mechanism in this interpretation does undoubtedly exist. However, this mechanism, though believed to be the main driving force of monsoon, has not been tested in numerical experiments. There has been an increasing recognition in the recent years that monsoon is inextricably tied to the heating in the intertropical convergence zone (ITCZ). We propose that the main cause of monsoon is ITCZ's being substantially away from the equator. A brief qualitative explanation of why the ITCZ can be a source of monsoon circulation can be offered based on the circulation field forced by the ITCZ heating. The existence of the ITCZ's does not always have to rely on land-sea contrast on the continental scale. This is hinted in the fact that in February the ITCZ close to Australia (and its associated monsoon circulation) covers a longitudinal range several times as long as that of Australia and thus cannot possibly be caused mainly by the land-sea contrast associated with Australia. Yet, this cannot be used as a proof that the ITCZ in the Asian summer monsoon is not mainly due to land-sea contrast. One of the purposes of this work is to provide a convincing proof. In this work the role of land-sea contrast in the origin of monsoon is examined through numerical simulation with the Goddard general circulation model. The Asian and Australian monsoon circulations are obtained in a four-year integration and then the integration is repeated with Asia, the maritime continent, and Australia replaced by ocean. The sea surface temperature (SST) at each affected grid is specified as the SST at the first grid to the east that is an ocean grid in the first experiment. The latter integration shows that the monsoon circulation pattern over where south Asia and Australia were and the surrounding region has largely remained. The results discount land-sea contrast as the main cause of Asian monsoon. A third experiment is the same as the first except that the topography of Asia, the maritime continent, and Australia is reduced to zero. This experiment reveals that the difference between the first two experiments is due more to the removal of topography than to the removal of land-sea contrast. August precipitation is shown averaged over the last three years of each of the three experiments. They show that the Asian monsoon rainy region is largely intact in the second experiment and the difference between the second and the third experiment is mainly in the longitudinal location of the maximum precipitation. Additionally, in Asian and Australian winter monsoons land-sea contrast also plays only a modifying role. Although land-sea contrast plays only a modifying role in Asian and Australian (and Central American including Mexican) monsoons, it is the main reason that ITCZ (and thus monsoon) exists in Africa and South America. Thus, monsoons can be classified into two groups depending on whether land-sea contrast plays a major role.

Description: In dynamic meteorology, singular vectors (SVs) are the structures that maximize a given norm of a forecast perturbation given a tangent linear model and a quadratic constraint on the initial perturbation. That constraint is a prescription of the value of either the same or a different norm applied to the initial perturbations. In the sense that SVs maximize the forecast perturbations according to a specified measure, they may be considered as optimal perturbations. SVs are used to characterize predictability, to identify and correct possible initial errors given forecast errors, to create a set of significant perturbations for ensemble forecasting, or to determine locations for observation targeting. They are a specific application of generally defined singular vectors in mathematics.

Description: Land-atmosphere feedback, by which precipitation-induced soil moisture anomalies affect subsequent precipitation, may be an important element of Earth's climate system, but its very existence has never been demonstrated conclusively at regional to continental scales. Evidence for the feedback is sought in a 50-year observational precipitation dataset covering the United States. The precipitation variance and autocorrelation fields are characterized by features that agree (in structure, though not in magnitude) with those produced by an atmospheric general circulation model (AGCM). Because the model-generated features are known to result from land-atmosphere feedback alone, the observed features are highly suggestive of the existence of feedback in nature.

Description: The South China Sea Monsoon Experiment (SCSMEX) was conducted in May-June 1998. One of its major objectives is to better understand the key physical processes for the onset and evolution of the summer monsoon over Southeast Asia and southern China. Multiple observation platforms (e.g., upper-air soundings, Doppler radar, ships, wind profilers, radiometers, etc.) during SCSMEX provided a first attempt at investigating the detailed characteristics of convective storms and air pattern changes associated with monsoons over the South China Sea region. SCSMEX also provided rainfall estimates which allows for comparisons with those obtained from the Tropical Rainfall Measuring Mission (TRMM), a low earth orbit satellite designed to measure rainfall from space. The Goddard Cumulus Ensemble (GCE) model (with 1-km grid size) is used to understand and quantify the precipitation processes associated with the summer monsoon over the South China Sea. This is the first (loud-resolving model used to simulate precipitation processes in this particular region. The GCE-model results captured many of the observed precipitation characteristics because it used a fine grid size. For example, the temporal variation of the simulated rainfall compares quite well to the sounding-estimated rainfall variation. The time and domain-averaged temperature (heating/cooling) and water vapor (drying/ moistening) budgets are in good agreement with observations. The GCE-model-simulated rainfall amount also agrees well with TRMM rainfall data. The results show there is more evaporation from the ocean surface prior to the onset of the monsoon than after the on-~et of monsoon when rainfall increases. Forcing due to net radiation (solar heating minus longwave cooling) is responsible for about 25% of the precipitation in SCSMEX The transfer of heat from the ocean into the atmosphere does not contribute significantly to the rainfall in SCSMEX. Model sensitivity tests indicated that total rain production is reduced 17-18% in runs neglecting the ice phase. The SCSMEX results are compared to other GCE-model-simulated weather systems that developed during other field campaigns (i.e., west Pacific warm pool region, eastern Atlantic region and central USA). Large-scale forcing vie temperature and water vapor tendency, is the major energy source for net condensation in the tropical cases. The effects of large-scale cooling exceed that of large-scale moistening in the west pacific warm pool region and eastern Atlantic region. For SCSMEX, however, the effects of large-scale moistening predominate. Net radiation and sensible and latent hc,it fluxes play a much more important role in the central USA.

Description: CloudSat is a satellite experiment designed to measure the vertical structure of clouds from space. The expected launch of CloudSat is planned for 2004 and, once launched, CloudSat will orbit in formation as part of a constellation of satellites including NASA's Aqua and Aura satellites, a NASA-CNES lidar satellite (P-C) and a CNES satellite carrying a polarimeter (PARASOL). A unique feature that CloudSat brings to this constellation is the ability to fly a precise orbit enabling the fields of view of the CloudSat radar to be overlapped with the P-C lidar footprint and the other measurements of the EOS constellation. The precision of this overlap creates a unique multi-satellite observing system for studying the atmospheric processes essential to the hydrological cycle. The vertical profile of cloud properties provided by CloudSat fills a critical gap in the investigation of feedback mechanisms linking clouds to climate. Measuring the vertical profile of cloud properties requires a combination of active and passive instruments, and this will be achieved by combining the radar data of CloudSat with active and passive data from other sensors of the constellation. This paper describes the underpinning science, and gives an overview of the mission, and provides some idea of the expected products and anticipated application of these products. Notably, the CloudSat mission is expected to provide new knowledge about global cloudiness, stimulating new areas of research on clouds including data assimilation and cloud parameterization. The mission also provides an important opportunity to demonstrate active sensor technology for future scientific and tactical applications. The CloudSat mission is a partnership between NASA/JPL, the Canadian Space Agency, Colorado State University, the US Air Force, and the US Department of Energy.

Description: Several heavy precipitation episodes occurred over Taiwan from August 10 to 13, 1994. Precipitation patterns and characteristics are quite different between the precipitation events that occurred from August 10 and I I and from August 12 and 13. In Part I (Chen et al. 2001), the environmental situation and precipitation characteristics are analyzed using the EC/TOGA data, ground-based radar data, surface rainfall patterns, surface wind data, and upper air soundings. In this study (Part II), the Penn State/NCAR Mesoscale Model (MM5) is used to study the precipitation characteristics of these heavy precipitation events. Various physical processes (schemes) developed at NASA Goddard Space Flight Center (i.e., cloud microphysics scheme, radiative transfer model, and land-soil-vegetation surface model) have recently implemented into the MM5. These physical packages are described in the paper, Two way interactive nested grids are used with horizontal resolutions of 45, 15 and 5 km. The model results indicated that Cloud physics, land surface and radiation processes generally do not change the location (horizontal distribution) of heavy precipitation. The Goddard 3-class ice scheme produced more rainfall than the 2-class scheme. The Goddard multi-broad-band radiative transfer model reduced precipitation compared to a one-broad band (emissivity) radiation model. The Goddard land-soil-vegetation surface model also reduce the rainfall compared to a simple surface model in which the surface temperature is computed from a Surface energy budget following the "force-re store" method. However, model runs including all Goddard physical processes enhanced precipitation significantly for both cases. The results from these runs are in better agreement with observations. Despite improved simulations using different physical schemes, there are still some deficiencies in the model simulations. Some potential problems are discussed. Sensitivity tests (removing either terrain or radiative processes) are performed to identify the physical processes that determine the precipitation patterns and characteristics for heavy rainfall events. These sensitivity tests indicated that terrain can play a major role in determining the exact location for both precipitation events. The terrain can also play a major role in determining the intensity of precipitation for both events. However, it has a large impact on one event but a smaller one on the other. The radiative processes are also important for determining, the precipitation patterns for one case but. not the other. The radiative processes can also effect the total rainfall for both cases to different extents.

Description: Six different convective-stratiform separation techniques, including a new technique that utilizes the ratio of vertical and terminal velocities, are compared and evaluated using two-dimensional numerical simulations of a tropical [Tropical Ocean Global Atmosphere Coupled Ocean-Atmosphere Response Experiment (TOGA COARE)] and midlatitude continental [Preliminary Regional Experiment for STORM-Central (PRESTORM)] squall line. The simulations are made using two different numerical advection schemes: 4th order and positive definite advection. Comparisons are made in terms of rainfall, cloud coverage, mass fluxes, apparent heating and moistening, mean hydrometeor profiles, CFADs (Contoured Frequency with Altitude Diagrams), microphysics, and latent heating retrieval. Overall, it was found that the different separation techniques produced results that qualitatively agreed. However, the quantitative differences were significant. Observational comparisons were unable to conclusively evaluate the performance of the techniques. Latent heating retrieval was shown to be sensitive to the use of separation technique mainly due to the stratiform region for methods that found very little stratiform rain. The midlatitude PRESTORM simulation was found to be nearly invariant with respect to advection type for most quantities while for TOGA COARE fourth order advection produced numerous shallow convective cores and positive definite advection fewer cells that were both broader and deeper penetrating above the freezing level.

Description: The Phanerozoic paleontological record is marked by several biological extinction events. One of them,at the Cretaceous/Tertiary (K/T) boundary was responsible for the demise of about 50% of genera and 75% of species, including the dinosaurs.These drastic and abrupt changes in the development of life on Earth puzzled paleontologists in the past. Many a cause was put forward to account for them, amongst them climate changes, disease, or overspecialization.

Description: The present invention involves methods and devices which enable discrete objects having a conducting inner core, surrounded by a dielectric membrane to be selectively inactivated by electric fields via irreversible breakdown of their dielectric membrane. One important application of the invention is in the selection, purification, and/or purging of desired or undesired biological cells from cell suspensions. According to the invention, electric fields can be utilized to selectively inactivate and render non-viable particular subpopulations of cells in a suspension, while not adversely affecting other desired subpopulations. According to the inventive methods, the cells can be selected on the basis of intrinsic or induced differences in a characteristic electroporation threshold, which can depend, for example, on a difference in cell size and/or critical dielectric membrane breakdown voltage. The invention enables effective cell separation without the need to employ undesirable exogenous agents, such as toxins or antibodies. The inventive method also enables relatively rapid cell separation involving a relatively low degree of trauma or modification to the selected, desired cells. The inventive method has a variety of potential applications in clinical medicine, research, etc., with two of the more important foreseeable applications being stem cell enrichment/isolation, and cancer cell purging.

Description: The matrix protein, M1, of influenza virus strain A/PR/8/34 has been purified from virions and crystallized. The crystals consist of a stable fragment (18 Kd) of the M1 protein. X-ray diffraction studies indicated that the crystals have a space group of P3.sub.t 21 or P3.sub.2 21. Vm calculations showed that there are two monomers in an asymmetric unit. A crystallized N-terminal domain of M1, wherein the N-terminal domain of M1 is crystallized such that the three dimensional structure of the crystallized N-terminal domain of M1 can be determined to a resolution of about 2.1 .ANG. or better, and wherein the three dimensional structure of the uncrystallized N-terminal domain of M1 cannot be determined to a resolution of about 2.1 .ANG. or better. A method of purifying M1 and a method of crystallizing M1. A method of using the three-dimensional crystal structure of M1 to screen for antiviral, influenza virus treating or preventing compounds. A method of using the three-dimensional crystal structure of M1 to screen for improved binding to or inhibition of influenza virus M1. The use of the three-dimensional crystal structure of the M1 protein of influenza virus in the manufacture of an inhibitor of influenza virus M1. The use of the three-dimensional crystal structure of the M1 protein of influenza virus in the screening of candidates for inhibition of influenza virus M1.

Description: This study used a two-dimensional coupled land/atmosphere (cloud-resolving) model to investigate the influence of land cover on the water budgets of squall lines in the Sahel. Study simulations used the same initial sounding and one of three different land covers, a sparsely vegetated semi-desert, a grassy savanna, and a dense evergreen broadleaf forest. All simulations began at midnight and ran for 24 hours to capture a full diurnal cycle. In the morning, the latent heat flux, boundary layer mixing ratio, and moist static energy in the boundary layer exhibited notable variations among the three land covers. The broadleaf forest had the highest latent heat flux, the shallowest, moistest, slowest growing boundary layer, and significantly more moist static energy per unit area than the savanna and semi-desert. Although all simulations produced squall lines by early afternoon, the broadleaf forest had the most intense, longest-lived squall lines with 29% more rainfall than the savanna and 37% more than the semi-desert. The sensitivity of the results to vegetation density, initial sounding humidity, and grid resolution was also assessed. There were greater differences in rainfall among land cover types than among simulations of the same land cover with varying amounts of vegetation. Small changes in humidity were equivalent in effect to large changes in land cover, producing large changes in the condensate and rainfall. Decreasing the humidity had a greater effect on rainfall volume than increasing the humidity. Reducing the grid resolution from 1.5 km to 0.5 km decreased the temperature and humidity of the cold pools and increased the rain volume.

Description: We provide preliminary evidence that existing algorithms for inferring small-scale gene regulation networks from gene expression data can be adapted to large-scale gene expression data coming from hybridization microarrays. The essential steps are (I) clustering many genes by their expression time-course data into a minimal set of clusters of co-expressed genes, (2) theoretically modeling the various conditions under which the time-courses are measured using a continuous-time analog recurrent neural network for the cluster mean time-courses, (3) fitting such a regulatory model to the cluster mean time courses by simulated annealing with weight decay, and (4) analysing several such fits for commonalities in the circuit parameter sets including the connection matrices. This procedure can be used to assess the adequacy of existing and future gene expression time-course data sets for determining transcriptional regulatory relationships such as coregulation.

Description: An extensive body of research this quarter is documented. Further methodical analysis of temperature residuals in Cryogenic Limb Array Etalon Spectrometer (CLAES) Version 8 level 3AT data show signatures during December 1992 at middle and high northern latitudes that, when compared to Naval Research Laboratory/Mountain Wave Forecast Model (NRL)/(MWFM) mountain wave hindcasts, reveal evidence of long mountain waves in these data over Eurasia, Greenland, Scandinavia and North America. The explicit detection of gravity waves in limb-scanned Cryogenic Infrared Spectrometers and Telescopes for the Atmosphere (CRISTA) temperatures is modeled at length, to derive visibility functions. These insights are used to convert CRISTA gravity wave temperature residuals into data that more closely resemble gravity wave fluctuations detected in data from other satellite instruments, such as Microwave Limb Sounder (MLS), Limb Infrared Monitor of the Stratosphere (LIMS) and Global Positioning System/Meteorology (GPS)/(MET). Finally, newly issued mesospheric temperatures from inversion of CRISTA 15gin emissions are analyzed using a new method that uses separate Kalman fits to the ascending and descending node data. This allows us to study global gravity wave amplitudes at two local times, 12 hours apart. In the equatorial mesosphere, where a large diurnal tidal temperature signal exists, we see modulations of gravity wave activity that are consistent with gravity wave-tidal interactions produced by tidal temperature variability.

Description: Since the advent of meteorological satellites in the 1960's, numerous experiments have been conducted in order to evaluate the impact of these and other data on atmospheric analysis and prediction. Such studies have included both OSE'S and OSSE's. The OSE's were conducted to evaluate the impact of specific observations or classes of observations on analyses and forecasts. Such experiments have been performed for selected types of conventional data and for various satellite data sets as they became available. (See for example the 1989 ECMWF/EUMETSAT workshop proceedings on "The use of satellite data in operational numerical weather prediction" and the references contained therein.) The ODYSSEY were conducted to evaluate the potential for future observing systems to improve Numerical Weather Prediction NWP and to plan for the Global Weather Experiment and more recently for EVANS (Atlas et al., 1985a; Arnold and Day, 1986; Hoffman et al., 1990). In addition, OSSE's have been run to evaluate trade-offs in the design of observing systems and observing networks (Atlas and Emmitt, 1991; Rohaly and Krishnamurti, 1993), and to test new methodology for data assimilation (Atlas and Bloom, 1989).

Description: The hypothesis being tested is that Super Dwarf wheat, Triticum aestivum L., plants in the Svet Greenhouse onboard the Russian Space Station Mir will complete a life cycle in spaceflight, providing that the environmental conditions necessary for adequate growth on Earth are supplied. Twenty six seeds of wheat were planted in each of 2 rows of 2 root compartments for a total of 104 seeds in Svet. Germination rate at 7 d was 56 and 73% on Mir and 75 and 90% in ground-based controls. Plants were grown throughout the whole cycle of ontogenesis (123 d) with samples gathered at different times to validate the morphological and reproductive stages of the plants. Young plants showed vigorous early seedling growth, with large biomass production, including the formation of 280 floral spikes. Upon return to Earth, comparative analyses showed that the number of tillers and flowers per spikelet were 63.2% and 40% greater, respectively, in Mir-grown plants than in the controls. By contrast, the stem length (52.4%), spike mass (49.2%) and length (23.1%), awn length (75.7%), number of spikelets per spike (42.8%) and number of seeds per spike (100% sterile) from Mir-grown plants were substantially less than the controls. Distribution of moisture and roots throughout the substrate was very good. All florets on Mir-grown spikes ceased development at the same stage of ontogeny. Lack of caryopses formation was attributed to male sterility occurring at different stages of staminal development. Anthers failed to dehisce and pollen grains were smaller and shriveled compared to the controls, suggesting a chronic stress had occurred in the Svet growth chamber. Recent ground-based studies indicated that ethylene, which was measured at 0.3 to 1.8 mg per kilogram in the Mir, almost certainly could have induced male sterility in the wheat plants grown on the Mir.

Description: Six different convective-stratiform separation techniques, including a new technique that utilizes the ratio of vertical and terminal velocities, are compared and evaluated using two-dimensional numerical simulations of a tropical [Tropical Ocean Global Atmosphere Coupled Ocean Atmosphere Response Experiment (TOGA COARE)] and midlatitude continental [Preliminary Regional Experiment for STORM-Central (PRESTORM)] squall line. Comparisons are made in terms of rainfall, cloud coverage, mass fluxes, apparent heating and moistening, mean hydrometeor profiles, CFADs (Contoured Frequency with Altitude Diagrams), microphysics, and latent heating retrieval. Overall, it was found that the different separation techniques produced results that qualitatively agreed. However, the quantitative differences were significant. Observational comparisons were unable to conclusively evaluate the performance of the techniques. Latent heating retrieval was shown to be sensitive to the use of separation technique mainly due to the stratiform region for methods that found very little stratiform rain.

Description: Hurricane Earl developed from a tropical wave that moved into the Gulf of Mexico, which triggered abundant convection. On 1 Sept. 1998, the wave was upgraded directly to a tropical storm. Earl reached hurricane status the next morning. The system moved erratically as it interacted with an upper level short wave trough rotating around a long wave trough to the northeast. The storm made landfall near 0600 UTC on 3 September near Panama City, FL. During August and September 1998, NASA conducted the Third Convection and Moisture Experiment (CAMEX-3). It focused on studying the intensity, track, and impacts at landfall of hurricanes. On the afternoon of 2 September 1998, the NASA ER2 high-altitude aircraft flying at 65,000 feet in tandem with the NASA DC-8 flying at 35,000 feet flew over and through, respectively, the eastern rainbands of Earl near the Florida Panhandle as the storm neared landfall in the region. Two approaches to studying Earl are undertaken here: first, an examination of the source and height of the dry air region using GOES-8 water vapor data and, second, a look into the impact of the dry air entrainment on the system using aircraft remote sensing data.

Description: A system and method for monitoring cellular activity in a cellular specimen. According to one embodiment, a plurality of excitable markers are applied to the specimen. A multi-photon laser microscope is provided to excite a region of the specimen and cause fluorescence to be radiated from the region. The radiating fluorescence is processed by a spectral analyzer to separate the fluorescence into respective wavelength bands. The respective bands of fluorescence are then collected by an array of detectors, with each detector receiving a corresponding one of the wavelength bands.

Description: A process for propagating a pathogen in a three-dimensional tissue mass cultured at microgravity conditions in a culture vessel containing culture media and a culture matrix is provided. The three-dimensional tissue mass is inoculated with a pathogen and pathogen replication in the cells of the tissue mass achieved.

Description: The penetration of the MARSIS radar signal into the polar ice mass is modeled to determine the capability of the instrument to locate sub-glacial aquifers. As a ground penetrating radar, the orbiting MARSIS transmits a signal greater than 1 W between 1-5 MHz. In this work we will investigate the effect of ice conductive losses on the radar-detection of subsurface aquifers. Based on wave propagation analysis, it is found that for a bulk ice conductivity below 10-5 S/m, conductive losses in the medium are not significant. However, if the bulk ice conductivity is relatively large (greater than 10-5 S/m), the reflected signal from any deep aquifer will be absorbed as it propagates in the lossy ice medium limiting the probing depth.

Description: Summer tropical synoptic-scale waves over West Africa are quantified by the 850 mb meridional wind component from the NCEP/NCAR reanalysis project. Their relationships with surface precipitation patterns are further explored by applying the data from the Tropical Rainfall Measuring Mission (TRMM) satellite in combination with other satellite observations during 1998-2002. Evident wavelet spectral power peaks are seen within a period of 2.5 - 6 days in both meridional wind and precipitation. The most intense wave signals in meridional wind are concentrated along 15 deg N- 25 deg N. Wave signals in precipitation and corresponding wavelet cross-spectral signals between these two variables, however, are primarily located at 5 deg N- 15 deg N, the latitudes of major summer rain events. There is a tendency for the perturbations in meridional wind component to lag (lead) precipitation signals south (north) of 15 deg N. In some cases, either an in-phase or out-of-phase relationship can even be found between these two variables, suggesting a latitude-dependent horizontal structure for these waves and probably implying two distinct wave-convective coupling mechanisms. Moreover, the lagging relationship (and/or the out-of-phase tendency) is only observed south of 15 deg N during July-September, indicating a strong seasonal preference. This phase relationship is generally consistent with the horizontal wave structures from a composite analysis.

Description: The development of a ground based direct detection Doppler lidar based on the recently described aerosol double edge technique is reported. A pulsed, injection seeded Nd:YAG laser operating at 1064 nm is used to make range resolved measurements of atmospheric winds in the free troposphere. The wind measurements are determined by measuring the Doppler shift of the laser signal backscattered from atmospheric aerosols. The lidar instrument and double edge method are described and initial tropospheric wind profile measurements are presented. Wind profiles are reported for both day and night operation. The measurements extend to altitudes as high as 14 km and are compared to rawinsonde wind profile data from Dulles airport in Virginia. Vertical resolution of the lidar measurements is 330 m and the rms precision of the measurements is a low as 0.6 m/s.

Description: Doctinary overlays on the definition of life can effectively be avoided by focusing discussion on microorganisms, their vital processes, and their genetic pedigree. To reach beyond these present and highly advanced forms of life and to inquire about its origin it is necessary to consider the requirements imposed by the environment. These requirements include geophysically and geochemically acceptable conjectures for the generation of source compounds, their concentration from dilute solution, and their selective combination into functional biomolecules. For vital function these macromolecules require programming in the form of specific sequence motifs. This critical programming constitutes the scientifically least understood process in the origin of life. Once this stage has been surpassed the laws of Darwinian evolution can operate in ways that are understood and experimentally demonstrated.

Description: RNA is adsorbed strongly on suspensions of many moderately soluble organic solids. In some cases, the hydrolysis of tRNA(sup Phe) is greatly accelerated by adsorption, and the major sites of hydrolysis are changed from those that are important in homogeneous solution. Here we show that the hydrolysis is greatly accelerated by suspensions of aspartic acid and beta-glutamic acid but not by suspensions of alpha-glutamic acid, asparagine, or glutamine. The non-enzymatic hydrolysis of RNA has been studied extensively, especially because of its relevance to the mechanisms of action of ribozymes and to biotechnology and therapy. Many ribonucleases, ribozymes, and non-biological catalysts function via acid-base catalysis of an intramolecular transesterification mechanism in which the 2'-OH group attacks the adjacent phosphate group. The pentacoordinated phosphorane intermediate may collapse back to starting material, or yield isomerized or cleaved products.

Description: Baroreceptors sense pressure in blood vessels and send this information to the brain. The primary baroreceptors are located in the main blood vessel leaving the heart (the aorta) and in the arteries in the neck (the carotid arteries). The brain uses information from the baroreceptors to determine whether blood pressure should be raised or lowered. These reflex responses are called baroreflexes. Changing position within a gravity field (i.e., moving from lying to sitting or standing) powerfully stimulates the baroreflexes. In weightlessness, the amount of stimuli that the baroreflexes receive is dramatically reduced. If this reduction occurs when the pathways that control the baroreflexes are being formed, it is possible that either the structure or function of the baroreceptors may be permanently changed. To study the effect of microgravity on structural and functional development of the aortic baroreflex system, we studied young rats (eight days old at launch) that flew on the Space Shuttle Columbia for 16 days. Six rats were studied on landing day; another six were studied after re-adapting to Earth's gravity for 30 days. On both landing day and 30 days after landing, we tested the sensitivity of the rats' baroreflex response. While the rats were anaesthetized, we recorded their arterial pressure, heart rate, and aortic nerve activity. After the tissues were preserved with perfusion fixation, we also examined the baroreflex structures. On landing day, we found that, compared to the controls, the flight rats had: fewer unmyelinated nerve fibers in their aortic nerves lower baroreflex sensitivity significantly lower contraction ability and wall tension of the aorta a reduced number of smooth muscle cells in the aorta. In the 30-day recovery group, the sensitivity of the baroreflex showed no difference between the flight rats and the control groups, although the unmyelinated fibers of the aortic nerve remained reduced in the flight rats. The results show that spaceflight does affect the development of the aortic baroreflex. The sensitivity of the reflex may be suppressed; however, the function of the blood pressure control system can re-adapt to Earth's gravity if the rats return before maturation. The structural differences in the input pathway of the reflex (Le., the reduction in nerve fibers) may remain permanently.

Description: The SeaWinds on QuikSCAT scatterometer was developed by NASA JPL to measure the speed and direction of ocean surface winds. Simulations performed to estimate the performance of the instrument prior to its launch have indicated that the mid-swath accuracy is worse than that of the rest of the swath. This behavior is a general characteristic of scanning pencil beam scatterometers. For SeaWinds, the accuracy of the rest of the swath, and the size of the swath are such that the instrument meets its science requirements despite mid-swath shortcomings. However, by understanding the problem at mid-swath, we can improve the performance there as well. We discuss the underlying causes of the problem in detail and propose a new wind retrieval algorithm which improves mid-swath performance. The directional discrimination ability of the instrument varies with cross track distance wind speed, and direction. By estimating the range of likely wind directions for each measurement cell, one can optimally apply information from neighboring cells where necessary in order to reduce random wind direction errors without significantly degrading the resolution of the resultant wind field. In this manner we are able to achieve mid-swath RMS wind direction errors as low as 15 degrees for low winds and 10 degrees for moderate to high winds, while at the same time preserving high resolution structures such as cyclones and fronts.

Description: Iron is an essential micronutrient for marine phytoplankton, limiting their growth in high nutrient, low chlorophyll regions of the ocean. I use a hierarchy of ocean circulation and biogeochemistry models to understand controls on global iron distribution. I formulate a mechanistic model of iron cycling which includes scavenging onto sinking particles and complexation with an organic ligand. The iron cycle is coupled to a phosphorus cycling model. Iron's aeolian source is prescribed. In the context of a highly idealized multi-box model scheme, the model can be brought into consistency with the relatively sparse ocean observations of iron in the oceans. This biogeochemical scheme is also implemented in a coarse resolution ocean general circulation model. This model also successfully reproduces the broad regional patterns of iron and phosphorus. In particular, the high macronutrient concentrations of the Southern Ocean result from iron limitation in the model. Due to the potential ability of iron to change the efficiency of the carbon pump in the remote Southern Ocean, I study Southern Ocean surface phosphate response to increased aeolian dust flux. My box model and GCM results suggest that a global ten fold increase in dust flux can support a phosphate drawdown of 0.25-0.5 micromolar.

Description: The onset of the southeast Asian monsoon during 1997 and 1998 was simulated with a coupled mesoscale atmospheric model (MM5) and a detailed land surface model. The rainfall results from the simulations were compared with observed satellite data fiom the TRMM (Tropical Rainfall Measuring Mission) TMI (TRMM Microwave Imager) and GPCP (Global Precipitation Climatology Project). The simulation with the land surface model captured basic signatures of the monsoon onset processes and associated rainfall statistics. The sensitivity tests indicated that land surface processes had a greater impact on the simulated rainfall results than that of a small sea surface temperature change during the onset period. In both the 1997 and 1998 cases, the simulations were significantly improved by including the land surface processes. The results indicated that land surface processes played an important role in modifying the low-level wind field over two major branches of the circulation; the southwest low-level flow over the Indo- China peninsula and the northern cold front intrusion from southern China. The surface sensible and latent heat exchange between the land and atmosphere modified the lowlevel temperature distribution and gradient, and therefore the low-level. The more realistic forcing of the sensible and latent heat from the detailed land surface model improved the monsoon rainfall and associated wind simulation.

Description: This quarter was largely devoted to a detailed study of temperature data acquired by the Cryogenic Limb Array Etalon Spectrometer (CLAES) on UARS. Our analysis used the same sequence of methods that have been developed, tested and refined on a more limited subset of temperature data acquired by the CRISTA instrument. We focused on a limited subset of our reasoning that geographical and vertical trends in the small-scale temperature variability could be compared with similar trends observed in November 1994 by the CRISTA-SPAS satellite. Results, backed up with hindcasts from the Mountain Wave Forecast Model (MWFM), reveal strong evidence of mountain waves, most persuasively in the Himalayas on 16-17 November, 1992. These CLAES results are coherent over the 30-50 km range and compare well with MWFM hindcasts for the same period. This constitutes, we believe, the first clear evidence that CLAES explicitly resolved long wavelength gravity waves in its CO2 temperature channel. A series of other tasks, related to mesoscale modeling of mountain waves in CRISTA data and fitting of ground-based and HRDI data on global scales, were seen through to publication stage in peer-reviewed journals.

Description: Colonization of space requires the ability to reproduce in reduced gravity. Following spaceflight, astronauts and male rats exhibit decreased testosterone (T). This has important implications as T effects the testes and accessory sex glands. To our knowledge no studies have examined the effects of spaceflight on accessory sex glands. Due to the rarity of spaceflight opportunities, ground models have been used to simulate weightlessness. The objective of this study was to determine the effect of long-term (21 d) weightlessness on the reproductive system of male rats. Weightlessness was simulated using the Morey-Holton hindlimb suspension (HLS) model. Age 10 week old, male Sprague-Dawley rats weighing (209.0 +9.7g) were randomly assigned (n=10/group) to either HLS or ambulatory control. In HLS rats, testes mass was 33% lower (p〈0.05) than ambulatory controls. However, HLS had no effect on prostate (0.65 +0.09g vs 0.69 +0.12g) or seminal vesicles (1.01 +0.35g vs 0.75 +0.22g) weights compared to controls. The absence of effects on plasma T in this study contrasts previous reports of reduced plasma T in HLS male rats. This discrepancy may have been due to the age of animal and timing of sampling. T levels vary dramatically during testes development as well as within normal diurnal cycles. In young HLS rats, testes weight was reduced but not plasma T. Subsequently there was no effect on accessory sex glands. However, this may not be the case in older rats. More studies using standardized methods are needed to gain a better understanding of male reproduction function and capability in weightlessness. Funding provided by NASA.

Description: Ideal cloud-resolving models contain little-accumulative errors. When their domain is so large that synoptic large-scale circulations are accommodated, they can be used for the simulation of the interaction between convective clouds and the large-scale circulations. This paper sets up a framework for the models, using moist entropy as a prognostic variable and employing conservative numerical schemes. The models possess no accumulative errors of thermodynamic variables when they comply with a discrete constraint on entropy conservation and sound waves. Alternatively speaking, the discrete constraint is related to the correct representation of the large-scale convergence and advection of moist entropy. Since air density is involved in entropy conservation and sound waves, the challenge is how to compute sound waves efficiently under the constraint. To address the challenge, a compensation method is introduced on the basis of a reference isothermal atmosphere whose governing equations are solved analytically. Stability analysis and numerical experiments show that the method allows the models to integrate efficiently with a large time step.

Description: Reasor et al. (2004) examined the tilting of a vortex by shear and found that a damping mechanism intrinsic to the dry adiabatic dynamics suppresses departures from an upright state. This realignment occurs through projection of the tilt asymmetry onto two types of vortex Rossby waves: sheared vortex Rossby waves, in which the radial shear of the swirling flow axisymmetrizes tilt asymmetries, and a quasi-mode, or discrete, vortex Rossby wave that, in the absence of damping, causes precession of the upper vortex until it realigns with the lower one (thereafter undergoing repeated cycles of tilting and realignment). With damping, the vortex achieves a downshear-left equilibrium tilt. This paper examines the role of a damped quasi-mode in producing asymmetric vertical motions in a high-resolution simulation of Hurricane Bonnie (1998).

Description: We determined if progressive differentiation of osteoblasts influences their sensitivity to gravitational loading. Osteoblasts were cultured for 4 days (confluent monolayer), 6 days (prenodules), 9 days (nodules) and 19 days (mineralized nodules), then centrifuged at 10 times gravity (g) or 50-g for 3 hours using the NASA Ames 1-ft. Diameter Centrifuge. Stationary controls were placed in an adjacent incubator. Following centrifugation, conditioned media were collected and analyzed for PGE, by ELISA. Microtubules were fluorescently labeled and analyzed by confocal microscopy to determine microtubule network morphology and height. Centrifugation at 10-g reduced microtubule network height by 15% on d4 and 10% on d6, with variable changes in more mature cultures. No major changes in microtubule morphology were observed. PGE(sub 2) release by d4 cultures increased in a dose-dependent fashion (3-fold at 10-g and 6-fold at 50-g relative to controls). D6 cultures produced a 5-fold increase for both 10-g and 50-g. PGE(sub 2) increased only 1.5-fold by d9, and by d19, PGE(sub 2) was not delectable in either the control or hypergravity-stimulated cells. Thus, as osteoblasts differentiate in culture, responsiveness of the microtubule cytoskeleton and the PGE(sub 2) pathway to hypergravity declines.

Description: Land-atmosphere feedback, by which (for example) precipitation-induced moisture anomalies at the land surface affect the overlying atmosphere and thereby the subsequent generation of precipitation, has been examined and quantified with many atmospheric general circulation models (AGCMs). Generally missing from such studies, however, is an indication of the extent to which the simulated feedback strength is model dependent. Four modeling groups have recently performed a highly controlled numerical experiment that allows an objective inter-model comparison of land-atmosphere feedback strength. The experiment essentially consists of an ensemble of simulations in which each member simulation artificially maintains the same time series of surface prognostic variables. Differences in atmospheric behavior between the ensemble members then indicates the degree to which the state of the land surface controls atmospheric processes in that model. A comparison of the four sets of experimental results shows that feedback strength does indeed vary significantly between the AGCMs.

Description: Mechanical forces generated by gravity, weightbearing, and muscle contraction play a key role in the genesis and maintenance of skeletal structure. The molecular mechanisms that mediate changes in osteoblast activity in response to altered patterns of skeletal loading are not known, and a better understanding of these processes may be essential for developing effective treatment strategies to prevent disuse osteoporosis. We have elucidated specific integrin/ECM (extracellular matrix) interactions that are required for osteoblast differentiation and survival and have developed a useful loading system to further explore the molecular basis of mechano-sensitivity of osteoblasts. The long term goal of our collaborative research is to understand how the ECM and cell adhesion proteins and integrins interaction to mediate the response of osteoblasts and their progenitors to mechanical loading. We suggest that integrin/ECM interactions are crucial for basic cellular processes, including differentiation and survival, as well as to participate in detecting and mediating cellular responses to mechanical stimuli.

Description: The One-Degree Daily (1DD) technique is described for producing globally complete daily estimates of precipitation on a 1 deg x 1 deg lat/long grid from currently available observational data. Where possible (40 deg N-40 deg S), the Threshold-Matched Precipitation Index (TMPI) provides precipitation estimates in which the 3-hourly infrared brightness temperatures (IR T(sub b)) are thresholded and all "cold" pixels are given a single precipitation rate. This approach is an adaptation of the Geostationary Operational Environmental Satellite (GOES) Precipitation Index (GPI), but for the TMPI the IR Tb threshold and conditional rain rate are set locally by month from Special Sensor Microwave/Imager (SSM/I)-based precipitation frequency and the Global Precipitation Climatology Project (GPCP) satellite-gauge (SG) combined monthly precipitation estimate, respectively. At higher latitudes the 1DD features a rescaled daily Television Infrared Observation Satellite (TIROS) Operational Vertical Sounder (TOVS) precipitation. The frequency of rain days in the TOVS is scaled down to match that in the TMPI at the data boundaries, and the resulting non-zero TOVS values are scaled locally to sum to the SG (which is a globally complete monthly product). The time series of the daily 1DD global images shows good continuity in time and across the data boundaries. Various examples are shown to illustrate uses. Validation for individual grid -box values shows a very high root-mean-square error but, it improves quickly when users perform time/space averaging according to their own requirements.