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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Microbial ecology 8 (1982), S. 281-283 
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 66 (1975), S. 1017-1023 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 45 (1991), S. 137-161 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 9 (1993), S. 493-494 
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1009
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1572-9729
    Keywords: analytical chemistry ; bioassay ; biochemistry ; composition ; hydrocarbons ; remediation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Bioremediation of petroleum-contaminated sites is expected to be a cost-effective remediation technology. However, many potential users of the technology expect the reliability of this technology to be similar to other candidate technologies for widespread consideration. In particular, candidate technologies should possess the property of reliable experimental linkage — there should be reasonable confidence that experiments done at one scale can be reliably related to another. An important example is bench-scale treatability studies that should result in linkages with commercial-scale operations. In this respect comparison of bioremediation to other candidate technologies reveals that bioremediation is in an early stage of its evolution. It is being pursued at a variety of sites and scales with practitioners from a variety of disciplines. Integration of activities between disciplines and an ability to quantitatively compare results at different sites and scales is proceeding. This paper addresses a number of physical, chemical, biological, analytical, and statistical issues regarding the successful comparison of results between experiments.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 10 (1992), S. 79-85 
    ISSN: 1476-5535
    Keywords: Bacterial containment ; Genetically engineered microbes ; hok ; gef ; Inducible promoters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Conditional lethal (suicidal) genetic constructs were designed and employed in strains of Pseudomonads as models for containment of geneticallyengineerd microbes that may be deliberately released into the environment. A strain ofPseudomonas putida was formed with a suicide vector designated pBAP24h that was constructed by cloning the host killing gene (hok) into the RSF1010 plasmid pVDtac24 and placing it under the control of thetac promoter. Afterhok induction inP. putida only 40% of surviving cells continued to bear thehok sequences within 4 h of induction; in contrast, 100% of the cells in uninduced controls borehok. A few survivors that demonstrated resistance tohok-induced killing developed inP. putida, which may have been due to a mutation or physiological adaptation that rendered the membrane ‘resistant’ tohok. Conditional lethal strains ofP. putida also were formed by insertinggef (a chromosomal homolog ofhok) under the control of thetac promoter into the chromosome using a transposon. Constructs with chromosomalgef, as well as an RK2-derived plasmid construct containinggef, were only marginally more stable than thehok constructs; they were effective in killingP. putida when induced and within 2 h post-induction killing from eithergef construct resulted in a 103−105-fold reduction in viable cell count compared to uninduced controls.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 4 (1989), S. 441-446 
    ISSN: 1476-5535
    Keywords: DNS hybridization ; Gene probe ; Environmental survival ; Pseudomonas cepacia AC1100 ; Alcaligenes A5
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The effectiveness of gene probe methods for tracking genetically engineered microorganisms (GEMs) in the environment was tested by inoculating nutrient-supplemented freshwater microcosms withAlcaligenes A5 (a naturally occurring 4-chlorobiphenyl degrader) orPseudomonas cepacia AC1100 (a genetically engineered 2, 4, 5 T-degrader) and following the fates of the introduced bacterial populations. Colony hybridization of the viable heterotrophic bacterial populations and dot blot hybridization of DNA recovered from the total microcosm microbial communities showed persistence of bothAlcaligenes A5 andP. cepacia AC1100 in the microcosms in the presence and absence of the xenobiotic substrates that these organisms biodegrade. Although there was a gradual decline in the added populations, both of the bacterial populatins were still detected in the microcosms two months after their introduction into the microcosms. Addition of 2, 4, 5-T enhanced the survival ofP. cepacia AC1100 — and 4-chlorobiphenyl addition resulted in increased levels ofAlcaligenes A5. The results indicate that both organisms may persist for very long periods in freshwater habitats.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1434-4483
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Summary  The relationship between European surface temperature and winds over the eastern North Atlantic are investigated for the years 1988 to 1997. Daily Special Sensor Microwave Imager SSM/I observations are used to evaluate a monthly surface wind index that quantifies the influence of southwesterly flow. Our wind index and the monthly-mean surface-air temperatures in late winter and early spring over France and northern-latitude Europe are highly correlated. In February, the year-to-year increases/decreases match every year for France (correlation of 0.82 with the Index); and every year with just one exception for Europe (correlation with the Index of 0.76 for a longitudinal strip through Europe 45–50° N, and 0.73 for the 50–60° N strip). In March, the increases/decreases of the wind Index and of the temperatures for France also match, but the correlation with the Index is lower, 0.65. The high correlation between our Index and the large interannual fluctuations in the monthly temperature in late winter and early spring indicate that the onset of the spring conditions in Europe is significantly influenced by the wind patterns over the eastern North Atlantic. Coinciding with the fluctuations from warm-Europe/high-Index winter to the opposite conditions, we observe “seesaw” effects, fluctuations over the North Atlantic, in opposite directions in the east (25–5° W), and the west (65–45° W). In the low-Index years we find that: (a) the surface-air temperatures in the west are appreciably higher than in the east (but slightly lower in the high-Index year), and (b) the difference between the 500 mb meridional wind in the west and that in the east is positive and large, exceeding 10 m s−1 (but it becomes negative and small in the high-Index years). The “seesaw” effects suggest that a positive feedback exits between these cross-Atlantic temperature differences and the surface winds.
    Type of Medium: Electronic Resource
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  • 10
    Publication Date: 2018-11-26
    Description: Experiments with a global observing system simulation experiment (OSSE) system based on the recent 7-km-resolution NASA nature run (G5NR) were conducted to determine the potential value of proposed Global Navigation Satellite System (GNSS) radio occultation (RO) constellations in current operational numerical weather prediction systems. The RO observations were simulated with the geographic sampling expected from the original planned Constellation Observing System for Meteorology, Ionosphere, and Climate-2 (COSMIC-2) system, with six equatorial (total of ~6000 soundings per day) and six polar (total of ~6000 soundings per day) receiver satellites. The experiments also accounted for the expected improved vertical coverage provided by the Jet Propulsion Laboratory RO receivers on board COSMIC-2. Except that RO observations were simulated and assimilated as refractivities, the 2015 version of the NCEP’s operational data assimilation system was used to run the OSSEs. The OSSEs quantified the impact of RO observations on global weather analyses and forecasts and the impact of adding explicit errors to the simulation of perfect RO profiles. The inclusion or exclusion of explicit errors had small, statistically insignificant impacts on results. The impact of RO observations was found to increase the length of the useful forecasts. In experiments with explicit errors, these increases were found to be 0.6 h in the Northern Hemisphere extratropics (a 0.4% improvement), 5.9 h in the Southern Hemisphere extratropics (a significant 4.0% improvement), and 12.1 h in the tropics (a very substantial 28.4% improvement).
    Print ISSN: 0027-0644
    Electronic ISSN: 1520-0493
    Topics: Geography , Geosciences , Physics
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