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  • Articles  (118)
  • wheat  (55)
  • Triticum aestivum  (49)
  • maize  (37)
  • Springer  (118)
  • 1990-1994  (118)
  • 1991  (118)
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  • Articles  (118)
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  • Springer  (118)
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  • 1990-1994  (118)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 58 (1991), S. 69-74 
    ISSN: 1570-7458
    Keywords: Rhopalosiphum padi ; antixenosis ; plant resistance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Antixenose gegenüberRhopalosiphum padi (L.) in den drei Weizensorten Timmo, Moghan 2 und Ommid, von denen die zwei letzteren Sorten aus dem Iran stammen, wurde unter Verwendung des systemischen Insektizids Pirimicarb gemessen. Jeweils zwei Pflanzen aus verschiedenen Sorten wurden als Paar verwendet (in allen Kombinationen der drei Sorten), pro Paar ein Topf mit Pirimicarb gegossen, und zwanzig Blattlaüse über Blattflächen von beiden Sorten gekäfigt. Ähnliche Versuche wurden auch ohne Insektizid ausgeführt. Beide Methoden zeigten deutliche Antixenose von Moghan 2 und Ommid in Vergleich mit Timmo. Die Insektizidmethode zeigte auch eine Bevorzugung von Moghan 2 gegenüber Ommid, ein Resultat, das mit der schon bekannten relativen Antibiose dieser zwei Sorten übereinstimmte, während sich ohne Insektizid kein solcher Unterschied erwies. Die statistische Analyse solcher Versuche mit einem Insektizid, die eine Modifikation der Berechnung des erwarteten Werts mit dem χ2 Test enthält, ist beschrieben.
    Notes: Abstract Antixenosis in three wheat varieties (Timmo, Moghan 2 and Ommid) toRhopalosiphum padi (L.) was measured by pairing plants of any two varieties with one plant of each pair treated with a soil drench of the systemic insecticide pirimicarb. Aphids were then allowed access to equivalent leaf areas from the two plants in a leaf cage. The results were compared with similar choice experiments without insecticide. By either technique, the two Iranian varieties (Moghan 2 and Ommid) showed antixenosis in comparison with Timmo. The statistical analysis of the data, using a heterogeneity χ2 test, is described.
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  • 2
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    Entomologia experimentalis et applicata 59 (1991), S. 79-85 
    ISSN: 1570-7458
    Keywords: Wheat ; Triticum aestivum ; cereals ; Rhopalosiphum padi ; Aphididae ; barley yellow dwarf virus ; hydroxamic acids ; DIMBOA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), a hydroxamic acid (Hx) occurring in wheat, was shown to deter feeding by the aphid Rhopalosiphum padi (L.), and to reduce BYDV transmission to the plant. Dual choice tests with wheat leaves showed the preferential settlement of aphids on leaves with lower levels of DIMBOA. Electric monitoring of aphid feeding behaviour showed that in seedlings with higher DIMBOA levels fewer aphids reached the phloem and they needed longer times to contact a phloem vessel than in those with lower levels. When aphids carrying BYDV were allowed to feed on wheat cultivars with different DIMBOA levels, fewer plants were infected with BYDV in the higher DIMBOA cultivars than in the lower ones. Preliminary field experiments showed a tendency for wheat cultivars with higher Hx levels to be more tolerant to infection by BYDV than lower Hx level ones.
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  • 3
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    Entomologia experimentalis et applicata 61 (1991), S. 169-177 
    ISSN: 1570-7458
    Keywords: African migratory locust ; crop environment ; oviposition behaviour ; oviposition sites ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Oviposition by the African migratory locust, Locusta migratoria migratorioides (Orthoptera: Acrididae), was studied in maize and wheat crops on the Orange Free State Highveld. Maize was shown to be the most important oviposition habitat with peak laying taking place in autumn and early winter when highest pod densities were recorded. Laying was mainly concentrated along the middle of the crop interrows in maize and within clearings in the wheat crop. Despite the uniform layout of these crops, the distribution of egg pods was found to be aggregated. Non-reproductive behaviour, such as locust aggregation, basking and feeding, as well as environmental factors appeared to influence the distribution of egg pods in these crops. Secondary selection for optinum soil moisture and compaction on the laying site enhanced the aggregation of pods.
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  • 4
    ISSN: 1432-0983
    Keywords: Light regulation ; psbN ; Triticum aestivum ; Etioplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of a region of wheat chloroplast DNA containing the psbB gene for the 47 kDa chlorophyll a-binding protein of photosystem II has been determined. The gene encodes a polypeptide of 508 amino acid residues which is predicted to contain six hydrophobic membrane-spanning regions. The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading frame of 38 codons is located between psbB and psbH, and on the opposite strand the psbN gene, encoding a photosystem II polypeptide of 43 amino acid residues, is located between orf38 and psbH. S1 nuclease mapping indicated that the 5′ ends of transcripts were located 371 and 183 bp upstream of the psbB translation initiation codon. Predominant transcripts of 2.1 kb and 1.8 kb for psbB and 0.4 kb for psbH were present in RNA isolated from etiolated and greening wheat seedlings. Immunodecoration of Western blots indicated that the 47 kDa polypeptide was absent, or present in very low amounts, in dark-grown tissue and accumulated on greening, whereas the 10 kDa polypeptide was present in similar amounts in both dark-grown and greening seedlings. The 10 kDa polypeptide was phosphorylated in vitro by incubating wheat etioplast membranes with [γ3 2P] ATP.
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  • 5
    ISSN: 1573-0832
    Keywords: Fusarium ; maize ; moniliformin ; mycotoxins ; trichothecenes ; zearalenone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Forty Fusarium isolates obtained from maize fields were screened for moniliformin production on maize kernels. Twelve isolates, including seven of F. subglutinans, were found to produce moniliformin at levels ranging from 0.4 to 64 ppm. Twenty six isolates were also screened for production of deoxynivalenol, diacetoxyscirpenol, T-2 toxin and zearalenone. Of these, 22, including all 11 isolates of F. graminearum, produced zearalenone at levels ranging from 0.1 to 96.0 ppm, while 13 produced T-2 toxin at low levels, (〈1.1 ppm). Deoxynivalenol and diacetoxyscirpenol were each produced by six isolates, also at low levels (〈1.0 ppm). Three isolates of F. graminearum and one of F. sambucinum produced four toxins simultaneously.
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  • 6
    ISSN: 1573-0832
    Keywords: Penicillium griseofulvum ; patulin ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sixty-four wheat samples from Spanish flour factories were screened for patulin and patulin-producing moulds. None of them was found to contain any patulin, whereas samples experimentally contaminated with this toxin proved it to be highly unstable. On the other hand, Penicillium griseofulvum was the only in vitro patulin-producing species found (19 samples). Mould growth in the samples was investigated by using yeast-sucrose medium (YES) and high-performance liquid chromatography (HPLC) to measure the amounts of toxin produced during 40 day's incubation at 20 and 28°C. The highest yield rate of patulin was obtained between the 20th and 30th day of incubation; such a rate, however, was very low throughout the vigorous growth phase, during the first 20 days of incubation. The more appropriate temperature for incubation and patulin production was 28 °C. We also investigated the influence of other incubation conditions in the yield and found stationary dark cultures to be more efficient that shaken or fermentation cultures in YES medium. The best patulin yield achieved was 11.9 mg in the culture broth and 6.3 mg in the mycelium from 100 ml of medium.
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  • 7
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    Nutrient cycling in agroecosystems 29 (1991), S. 163-172 
    ISSN: 1573-0867
    Keywords: Superphosphate ; residual value ; sandy soil ; leaching of phosphorus ; lupins ; barley ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on deep, yellow, sandy soil near Badgingarra, Western Australia, the residual value of superphosphate applied one and two years previously was measured relative to freshly-applied superphosphate using yields of narrow-leafed lupin (Lupinus angustifolius), barley and wheat. In addition, soil samples were collected for measurement of bicarbonate-extractable soil P. This was also used to estimate the residual value of the superphosphate. For lupins and wheat, and for bicarbonate-extractable soil P, the residual value decreased with increasing level of application. For barley grain, the residual value was not significantly affected by the level of application. The decrease in residual value of superphosphate with increasing level of application is attributed to increased leaching of applied phosphorus (P) down the profile of the sandy soils as the level of application increases. This may reduce subsequent plant yields due to the delay in seedling roots reaching the P in the soil during the crucial early stages of plant growth. For lupins, the relationship between yield and the level of superphosphate applied was markedly sigmoidal. The relationship for wheat and barley was exponential. Consequently, at suboptimal levels of P application, lupins required about two to three times more P than wheat or barley to produce the same yield. However, lupins required less P to achieve near-maximum yield.
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  • 8
    ISSN: 1432-203X
    Keywords: wheat ; rye ; embryogenesis ; growth ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the short arm of rye chromosome 1 (1RS) from Secale cereale var. Imperial on the growth and differentiation of callus cultures from wheat Triticum aestivum var. Chinese Spring immature embryos was analysed. This chromosome arm was found to stimulate both embryogenesis and the rate of growth of calli. Recombinant lines carrying segments of 1RS were used to delineate the regions of 1RS responsible for the tissue culture effects. The enhancement of embryogenesis and the stimulation of growth were shown to be associated with two distinct genetic regions of the chromosome arm; the former is located between the centromere and the Sec 1 locus, while the latter is situated in the immediate vicinity of the Sec 1 locus.
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  • 9
    ISSN: 1432-0983
    Keywords: Mitochondrion ; Transcription ; Guanylyltransferase ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To identify transcription initiation sites in wheat mitochondria, the nascent 5′-ends of transcripts were specifically labeled by incubation of wheat mitochondrial RNA with [α-32P]GTP in the presence of the enzyme guanylyltransferase. After separation of the resulting capped transcripts by electrophoresis in polyacrylamide gels, individual RNAs were recovered and directly sequenced. Four RNA sequences obtained in this way were localized upstream of the protein-coding genes atpA, coxII, coxIII and orf25. Comparison of mRNA and gene sequences allowed precise positioning of transcription initiation sites for these four genes. Sequence similarities immediately upstream of these sites define a conserved motif that we suggest as a candidate regulatory element in wheat mtDNA. The relationship between this motif and putative mitochondrial promoters in other plant species is discussed.
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  • 10
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    Plant molecular biology 16 (1991), S. 919-923 
    ISSN: 1573-5028
    Keywords: Zea mays ; maize ; embryogenesis ; abscisic acid ; seed maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated by differential hybridization a cDNA, termed Emb564, which is complementary to an 800 nt embryo-specific transcript in Zea mays. The expression of Emb564 can be enhanced by exogenous abscisic acid (ABA) and the effect of ABA on the accumulation of Emb564 transcript appears to be confined to embryos at the early and mid embryonic stages. In addition, Emb564 is expressed at low levels in ABA-deficient but not in ABA non-responsive embryos. Genomic analysis suggested that the Emb564 mRNA is encoded by a single gene. Sequence analysis showed that Emb564 exhibits extensive similarities with several known ABA-inducible genes.
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  • 11
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    Plant molecular biology 16 (1991), S. 335-337 
    ISSN: 1573-5028
    Keywords: Triticum ; wheat ; endosperm ; gliadin ; pseudogene ; duplication ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 12
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    Plant molecular biology 16 (1991), S. 907-908 
    ISSN: 1573-5028
    Keywords: ubiquitin ; wheat ; heat shock protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 13
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    Plant molecular biology 16 (1991), S. 935-936 
    ISSN: 1573-5028
    Keywords: maize ; mitochondrial DNA ; repeated sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 14
    ISSN: 1573-5028
    Keywords: α-amylase inhibitor ; expression inE. coli ; glycosylation versus activity ; insect α-amylase ; mutagenesis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wheat monomeric inhibitor WMAI-1 (syn. 0.28) produced inEscherichia coli using the pT7-7 expression ventor has the correct N-terminal sequence and the same electrophoretic mobility and specific activity towards the α-amylase from the insectTenebrio molitor as the native WMAI-1 isolated from wheat. This confirms that the native inhibitor is not glycosylated and contradicts claims that a putative glycosyl moiety was essential for inhibition. Thirteen mutants have been obtained at six different sites. Substitution of the highly conserved N-terminal S by the sequence ARIRAR increased the pre-incubation time required for maximum activity. A similar result was obtained by insertion of GPRLPW after position 4, while insertion of EPRAPW at the same position rendered the inhibitor inactive. The substitution D/EGPRL and insertions DGP or D, at position 58, produced complete inactivation. All other mutations had only minor effects on activity.
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  • 15
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    Plant molecular biology 17 (1991), S. 273-275 
    ISSN: 1573-5028
    Keywords: Tahsp17.3 ; low-molecular-weight HSP ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 16
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    Plant molecular biology 17 (1991), S. 1259-1261 
    ISSN: 1573-5028
    Keywords: anaerobic genes ; maize ; pyruvate decarboxylase ; DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 17
    ISSN: 1573-5028
    Keywords: pathogen-induced ; peroxidase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here the complete amino acid sequence of a pathogen-induced putative peroxidase from wheat (Triticum aestivum L.) as deduced from cDNA clones representing mRNA from leaves infected with the powdery mildew fungus Erysiphe graminis. The protein consists of 312 amino acids, of which the first 22 form a putative signal sequence, and has a calculated pI of 5.7. Sequence comparison revealed that the putative wheat peroxidase is most similar to the turnip (Brassica rapa) peroxidase, with which it shares 57% identical and 13% conserved amino acids.
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  • 18
    ISSN: 1573-5028
    Keywords: peroxidase gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic libary of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen-nor wound-induced in leaves but is constitutively expressed in roots.
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  • 19
    ISSN: 1573-5028
    Keywords: anaerobic stress ; enolase ; gene regulation ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA encoding maize enolase (2-phospho-D-glycerate hydrolase) was purified by functional genetic complementation using an enolase deficient mutant of Escherichia coli, DF261. This cDNA, pZM245, was characterized by restriction mapping and DNA sequence analysis. The cDNA contained an open reading frame encoding a protein of 446 amino acids with a high degree of similarity to enolase sequences from other organisms (72% identity to yeast enolase and 82% identity to human enolase). The pZM245 contains a correctly positioned consensus prokaryotic translation initiation sequence. The specific activity of enolase in maize increases to about twice its initial level after 48 hours of anaerobiosis. Northern-blot analysis showed a five-fold anaerobic induction in enolase mRNA, while heat shock or cold shock increased enolase mRNA levels only slightly. Southern-blot analysis of maize genomic DNA indicated that there is one copy of the pZM245 hybridizing sequence per haploid genome in maize.
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  • 20
    ISSN: 1573-5028
    Keywords: cDNA family ; maize ; pollen-expressed genes ; polygalacturonase ; signal peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA clone, named PG1, abundantly expressed in late stages of pollen development, has been isolated from a cDNA library using a differential screening method with cDNA probes representative of microspores at early or late developmental stages. The encoded 410 amino acid polypeptide has significant homology with various polygalacturonases (PG) described elsewhere. Two polypeptides, of 49 and 53 kDa respectively, have been identified in the active PG fraction, isolated from mature pollen by immuno-cross-reaction with tomato PG antibodies. According to their N-terminal sequence, they can be identified as being mature peptides encoded by the PG1 cDNA clone. We propose that these two proteins derive from a unique precursor through several post-translational events, including the excision of a 22 amino-terminal signal peptide and glycosylation. PG-encoding genes form a small genomic family. Sequence analysis of three PG cDNA clones shows that they are closely related. The divergence of nucleotides between these three cDNA clones is 1%. They encode the same product.
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  • 21
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    Plant molecular biology 16 (1991), S. 1073-1076 
    ISSN: 1573-5028
    Keywords: abscisic acid ; dehydration ; LEA ; water stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475–486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23 000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.
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  • 22
    ISSN: 1573-5028
    Keywords: expression ; germination ; histone genes ; maize ; subfamilies ; tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The steady-state levels of H3 and H4 mRNAs transcribed from three H3 and two H4 multigene subfamilies were studied during germination and in different organs of maize. During germination the five subfamilies are expressed in parallel to DNA synthesis, but a 5-fold difference in the quantity of mRNAs transcribed per gene copy was found from our subfamily to another. In adult plants H3 and H4 mRNA levels are highest in organs containing meristematic tissues but also high in non-proliferating tissues. No strict tissue specificity expression could be detected but some subfamilies show preferential expression in some tissues.
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  • 23
    ISSN: 1573-5028
    Keywords: germination ; fungi ; maize ; pathogenesis-related ; seed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pathogenesis-related proteins (PRs) are plant proteins produced in leaves in response to infection by pathogens including viruses, viroids, fungi and bacteria. Information on the presence and/or expression of PRs in monocotyledonous plants is scarce. Here we report the identification of cDNA and genomic clones coding for a basic form of a protein from germinating maize seeds having a high homology with the group of PR-1 from tobacco. A cDNA library enriched in aleurone-specific sequences was prepared from maize seeds two days after germination. One clone was found to contain an open reading frame encoding a protein homologous to PR proteins from tomato (p14) and tobacco (PR-1 group). Sequence analysis of the corresponding genomic clone revealed that it was encoded by a single exon. Besides, DNA blot hybridization indicates that this PR-like protein is encoded by a single-copy gene in maize. The accumulation of its mRNA increases after rehydration of desiccated seeds. Furthermore, a relationship was found between its expression and infection by a natural pathogen of maize, the fungus Fusarium moniliforme. The possible role of this protein as a response mechanism following fungal infection in cereal seeds is discussed.
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  • 24
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    Plant molecular biology 16 (1991), S. 663-670 
    ISSN: 1573-5028
    Keywords: gene expression ; proline-rich protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich proteins. The WPRP1 cDNA clone was used to determine the copy number and chromosomal location of the WPRP1 gene by restriction fragment length polymorphism analysis of wheat inbred lines. Although WPRP1 is encoded by a single-copy gene it is also a representative of a larger family of related sequences. RNA gel blot analysis showed that expression of WPRP1 is highest in rapidly growing tissue which together with its amino acid composition suggests a structural role for the encoded protein.
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  • 25
    ISSN: 1573-5028
    Keywords: heat shock ; heat shock cDNAs ; maize ; small heat shock proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heat-shocked maize seedlings (cv. Oh43) synthesize a characteristic set of heat-shock proteins (hsps) which include an 18 kDa family containing at least six major isoelectric variants. A cDNA library was constructed from poly(A)+ RNAs isolated from the radicles of heat-shocked maize seedlings and screened with a DNA fragment from the theoretical open reading frame of a putative Black Mexican Sweet maize hsp 18 genomic clone. Two clones, cMHSP18-3 and cMHSP18-9, were isolated, and the RNA transcripts generated from them were translated into proteins which immunoreact with antibodies directed against the maize 18 kDa hsps and exhibit the same electrophoretic characteristics as two different members of the 18 kDa hsp family. Nucleotide sequence analyses of the cDNAs in these clones reveal that their 5′ and 3′ untranslated regions exhibit 33–34% identity and that their protein encoding regions share 93% identity. The deduced amino acid sequences of these clones show 90% identity, and the apparent molecular masses and isoelectric points of these proteins agree with those established for two different 18 kDa hsps, numbered 3 and 6. This report substantiates that at least two of the 18 kDa hsps in maize are products of different but related genes. Moreover, it establishes that transcripts for these proteins accumulate during heat shock and that both their nucleotide and deduced amino acid sequences share extensive similarities with the class VI small hsps in soybean and with transcripts expressed during meiosis in Lilium.
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  • 26
    ISSN: 1573-5028
    Keywords: chloroplast genes ; cytochrome b6 ; introns ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
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  • 27
    ISSN: 1573-5028
    Keywords: enhanced gene expression ; exon ; intron ; maize ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Both exon 1 and intron 1 of the maize Shrunken-1 (Sh1) gene individually stimulate expression of reporter genes in transient gene expression experiments if present within the transcription unit. The Sh1 exon 1 mediates a 10-fold increase in activity when inserted at the 5′ end of the bacterial chloramphenicol transacetylase (CAT) marker gene in both monocot and dicot protoplasts. The Sh1 intron 1 enhances chimeric gene expression in rice and maize protoplasts approximately 100-fold but inhibits CAT expression in tobacco protoplasts. In combination, the stimulatory effects of Sh1 exon 1 and intron 1 are multiplicative in monocot protoplasts resulting in a final enhancement of up to 1000-fold compared to the unmodified CAT or luciferase marker genes.
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  • 28
    ISSN: 1573-5028
    Keywords: wheat ; glutathione-S-transferase ; transposon-like sequence
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  • 29
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    Plant molecular biology 16 (1991), S. 1099-1101 
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; waxy protein
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  • 30
    ISSN: 1573-5028
    Keywords: anthocyanin ; B-Peru ; maize ; transcription activator
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  • 31
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    Plant molecular biology 17 (1991), S. 167-168 
    ISSN: 1573-5028
    Keywords: chloroplast ; monocot gene ; phosphoribulokinase ; wheat
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  • 32
    ISSN: 1573-5028
    Keywords: Tahsp26.6 ; chloroplast-localized HSP ; Triticum aestivum L. ; wheat
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  • 33
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    Plant molecular biology 17 (1991), S. 305-307 
    ISSN: 1573-5028
    Keywords: adenine nucleotide translocator ; ADP/ATP translocase ; mitochondria ; maize ; inner mitochondrial membrane
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  • 34
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    Plant molecular biology 17 (1991), S. 361-369 
    ISSN: 1573-5028
    Keywords: chloroplast genome ; cloning in YACs ; maize ; stability analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 139 kb circular maize chloroplast genome was cloned in a linear form in a single yeast artificial chromosome (YAC), and several such clones were then analyzed to study the stability of large DNA sequences in YACs. The YAC clones were isolated from a partial maize DNA YAC library. The library was constructed byBal-31 nuclease digestion of high-molecular-weight total maize genomic DNA followed by blunt-end ligation into a modified YAC vector, pYAC11. All chloroplast genome-containing YACs (cpYACs) were characterized by comparing their restriction enzyme digestion patterns with that of purified maize chloroplast DNA (MCD). Comparison of the restriction patterns of four cpYACs, MY503, MY504, MY518 and MY526, with that of MCD did not show any change in the size of the restriction fragments except at the chloroplast DNA-vector junction fragments. These altered size fragments were confirmed as junction fragments by hybridization of the pYAC11 border fragments. The end-specific T3 and T7 transcripts synthesized from MY503 also identified the same junction fragments as did the pYAC11 border fragments. Most cpYACs were completely stable through the analyzed one hundred generations.
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  • 35
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    Plant molecular biology 17 (1991), S. 679-690 
    ISSN: 1573-5028
    Keywords: herbicide safener ; maize ; inducible plant genes ; differential gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A search of compounds capable of inducing specific gene expression in plants without affecting growth and development led to the examination of changes in the pattern of gene expression in corn after treatment with substituted benzenesulfonamide herbicide safeners. Following hydroponic treatment of corn with the safener N-(aminocarbonyl)-2-chlorobenzenesulfonamide (2-CBSU), the specific induction of new translatable mRNA species was observed. Replicate copies of a cDNA library made using RNA from 2-CBSU-treated corn roots were differentially screened with cDNA probes made from either the same mRNA fraction used for library construction or mRNA isolated from roots treated with 2-chlorobenzenesulfonamide (2-CBSA), an inactive analog of the safener. Colonies showing hybridization only with the probe made using mRNA from 2-CBSU-treated roots were further characterized to assess the specificity of the induction and decay of the corresponding induced RNA species. RNA blot analyses showed two clones, designated In2-1 and In2-2, contained plasmids that hybridized to RNAs that were induced from an undetectable background in corn roots within 30 minutes after treatment with 2-CBSU. Leaf and meristem tissues showed similar inductions of the In2-1 and In2-2 RNA species after a delay of several hours. In addition, both RNA species were induced in corn by foliar application of 2-CBSU. In contrast, neither RNA species was induced following stress treatments of plants. These results indicate a substituted benzenesulfonamide safener might be used with the promoters from the In2-1 and In2-2 genes to develop a new inducible gene expression system for plants.
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  • 36
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    The journal of membrane biology 121 (1991), S. 11-22 
    ISSN: 1432-1424
    Keywords: channel ; protoplast ; K+ current ; patch clamp ; corn ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Whole-cell sealed-on pipettes have been used to measure electrical properties of the plasmalemma surrounding protoplasts isolated from Black Mexican sweet corn shoot cells from suspension culture. In these protoplasts the membrane resting potential (V m ) was found to be −59±23 mV (n=23) in 1mm K o − . The meanV m became more negative as [K−] o decreased, but was more positive than the K+ equilibrium potential. There was no evidence of electrogenic pump activity. We describe four features of the current-voltage characteristic of the plasmalemma of these protoplasts which show voltagegated channel activity. Depolarization of the whole-cell membrane from the resting potential activates time- and voltage-dependent outward current through K+-selective channels. A local minimum in the outward current-voltage curve nearV m =150 mV suggests that these currents are mediated by two populations of K+-selective channels. The absence of this minimum in the presence of verapamil suggests that the activation of one channel population depends on the influx of Ca2+ into the cytoplasm. We identify unitary currents from two K+-selective channel populations (40 and 125 pS) which open when the membrane is depolarized; it is possible that these mediate the outward whole-cell current. Hyperpolarization of the membrane from the resting potential produces time- and voltage-dependent inward whole-cell current. Current activation is fast and follows an exponential time course. The current saturates and in some cases decreases at membrane potentials more negative than −175 mV. This current is conducted by poorly selective K+ channels, whereP Cl/P K=0.43±0.15. We describe a low conductance (20 pS) channel population of unknown selectivity which opens when the membrane is hyperpolarized. It is possible that these channels mediate inward whole-cell current. When the membrane is hyperpolarized to potentials more negative than −250 mV large, irregular inward current is activated. A third type of inward whole-cell current is briefly described. This activates slowly and with a U-shaped current-voltage curve over the range of membrane potentials −90〈V m 〈0 mV.
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  • 37
    ISSN: 1432-2242
    Keywords: Preferential transmission ; Triticum aestivum ; Aegilops sharonensis
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    Topics: Biology
    Notes: Summary The transmission of chromosome 4S l from Aegilops sharonensis was observed in a range of wheat genetic backgrounds. Chromosome 4S l was transmitted at a very high frequency (at least 97.8%) in all crosses. The genetic background appears to only have a small effect on transmission. The frequency of transmission of chromosome 4S l was the same in each genetic background through both the male and female gametes.
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  • 38
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    Theoretical and applied genetics 81 (1991), S. 576-580 
    ISSN: 1432-2242
    Keywords: Pollen culture ; Pollen maturation ; Pollination ; Triticum aestivum ; Gliadins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immature pollen of two varieties of Triticum aestivum, at the stage right after the first pollen mitosis, was isolated from individual anthers and cultured in microcultures of microliter droplets. In a specifically designed medium, some of the pollen grains developed to maturity. These were applied to excised stigmas on agar, where they produced pollen tubes. Application to flowers in vivo led to seed set. Pollen was matured in vitro from a variety that produced a different protein banding pattern on SDS-PAGE as compared to the variety that was pollinated. The protein banding in the produced seeds showed the hybrid pattern, demonstrating that the seeds were not produced by self-pollination in this in-breeding species but by pollination with the in-vitro-matured pollen.
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  • 39
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    Theoretical and applied genetics 82 (1991), S. 615-620 
    ISSN: 1432-2242
    Keywords: HSP-70 ; BiP/GRP-78 ; Wheat ; Triticum aestivum ; Chaperone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Animal and plant cells contain a family of constitutively expressed HSP-70 cognate proteins that are localized in different subcellular locations and are presumed to play a role in protein folding and transport. Utilizing antibodies raised against the yeast endoplasmicreticulum-localized HSP-70 cognate termed BiP/GRP-78, as well as antibodies raised against the Escherichia coli HSP-70 protein DnaK, we have identified and characterized a large family of closely related proteins in wheat. One protein band of 78 kDa that is apparently closely related to yeast BiP was localized in the endoplasmic reticulum. This band cross-reacted with the yeast BiP but not with the DnaK-specific antibodies. The yeast BiP antibodies also recognized a cytoplasmic protein of 70 kDa that is probably related to the HSC-70 cognate proteins. These two proteins were further confirmed as HSP-70 cognates by their ability to bind to an ATP-agarose column. Probing of proteins from purified wheat mitochondrial preparations with the yeast BiP and DnaK-specific antibodies showed that this organelle contained a family of HSP-70-related proteins. The yeast BiP antibodies recognized two mitochondrial proteins of 60 and 58 kDa, but failed to detect any protein in the size rang of 70 to 80 kDa. However, the presence of immunologically distinct proteins of 90 and 78 kDa, as well as of lower molecular weight from this family in the mitochondria, was shown by probing with the DnaK-specific antibodies. A new protein of 30 kDa, cross-reacting with anti-yeast BiP antibodies, was detected only in developing seeds, close to their maturity. The evolution of HSP-70 cognate proteins in wheat as shown in this study is discussed.
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  • 40
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    Theoretical and applied genetics 82 (1991), S. 674-680 
    ISSN: 1432-2242
    Keywords: Puccinia recondita tritici ; Triticum aestivum ; Rust resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A study of spring bread wheat (Triticum aestivum) germ plasm developed at the International Maize and Wheat Improvement Center (CIMMYT) showed highly significant phenotypic variability for each component of partial resistance (namely, uredial appearance period, latency period, uredial number and uredial size) to Puccinia recondita f. sp. tritici. All of the wheat genotypes displayed longer uredial appearance and latency periods and decreased uredial number and uredial size when compared to the susceptible check cultivar ‘Morocco’. Positive correlations between uredial appearance period and latency period, and uredial number and uredial size, and negative correlations between uredial appearance and latency periods and uredial number and uredial size, inclusive, suggested that the components of partial resistance were either tightly linked or under pleiotropic genetic control. Compared to ‘Morocco’, all entries had slow disease progress in the field and variation occurred in the germ plasm for the area under the leaf rust progress curve. Disease progress was negatively correlated with uredial appearance and latency periods, whereas a positive correlation was observed with uredial number and uredial size. Certain genotypes displayed high levels of partial resistance resulting in low disease incidence in the field.
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  • 41
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    Theoretical and applied genetics 81 (1991), S. 312-316 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron michnoi ; Intergeneric hybrid ; Chromosome pairing ; Self-fertile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids between Triticum aestivum cv ‘Chinese Spring’ (2n=6x=42, AABBDD) and Agropyron michnoi Roshev. (2n=4x=28, PPPP) were obtained by embryo culture. Their spike characteristics were similar to those of common wheat but, unlike their parents, they were long-awned. The average meiotic chromosome pairing at MI of F1 hybrids was: 6.39 I +3.75 rodII+8.64 ringII+0.81 III+0.30 IV+0.04 V, the bivalent and multivalent formation of which was much higher than expected from the genomic formulae. It is especially worthwhile to note that the F1 hybrids were self-fertile, self set being 0.15%, and seeds were easily obtained from the backcross of f1 plants with hexaploid and tetraploid wheats; here the seed set was more than 20.0%. The polyploid taxa and the position of A. Michnoi in Agropyron are discussed.
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  • 42
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Triticum monococcum ; blue aleurone color ; Chromosome substitution/translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiotic chromosome pairing and Giemsa C-banding analyses in crosses of several European blue-grained wheat strains with Chinese Spring double ditelosomic and other aneuploid lines showed that Triticum aestivum Blaukorn strains “Berlin,” “Probstdorf,” “Tschermak,” and “Weihenstephan” are chromosome substitutions, in which the complete wheat chromosome 4A pair is replaced, whereas the strains “Brünn” and “Moskau” are 4B substitutions. The alien chromosome pair in all of these strains is an A genome chromosome (4A) from diploid Triticum monococcum or T. boeoticum not present in common tetraploid and hexaploid cultivated wheats. The Blaukorn strain Weihenstephan “W 70a86” possesses, in addition to a rye chromosome pair 5R compensating for the loss of part of chromosome 5D, a 4A/5DL translocation replacing chromosome pair 4B of wheat.
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  • 43
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    European journal of plant pathology 97 (1991), S. 275-288 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; brown rust ; monitoring ; epidemiology ; yield loss ; disease management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Epidemics ofPuccinia recondita and resulting yield loss of winter wheat were studied in field experiments over three seasons in the Netherlands. Results are reported and used to construct an advisory model for control of brown rust, based on rust monitoring. If the fraction of leaves with rust (I) at a certain development stage is determined, the average number of rust sori per leaf (M) was estimated by: M=EXP(1.84+1.39ln(ln[1/(1-I)]). The final number of sori per leaf (Mf) at early dough was forecast by an exponential growth: Mf=M·EXP(RGR·t). The relative growth rate (RGR) averaged 0.163/day and the forecasting period, t, until early dough, was derived from published data. The forecast number of sori-days per leaf (S, AUDPC-value) was then obtained by: S=(Mf-M)/RGR. Yield loss (kg/ha) by brown rust was 1.15 times the number of sori-days per leaf at low rust intensities. The efficacy of the fungicides used was 85%. The forecast avoidable yield loss (L, kg/ha) was calculated by: L=0.85 (1.15 S). Economic thresholds for brown rust control at different development stages are given for Dutch wheat fields at a cost level of 270 kg/ha for one fungicide application.
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  • 44
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    Plant and soil 131 (1991), S. 59-66 
    ISSN: 1573-5036
    Keywords: maize ; mechanical stress ; penetrometer ; root growth ; sandy loam
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root penetration resistance and elongation of maize seedling roots were measured directly in undisturbed cores of two sandy loam soils. Root elongation rate was negatively correlated with root penetration resistance, and was reduced to about 50 to 60% of that of unimpeded controls by a resistance of between 0.26 and 0.47 MPa. Resistance to a 30° semiangle, 1 mm diameter penetrometer was between about 4.5 and 7.5 times greater than the measured root penetration resistance. However, resistance to a 5° semiangle, 1 mm diameter probe was approximately the same as the resistnace to root penetration after subtracting the frictional component of resistance. The diameter of roots grown in the undisturbed cores was greater than that of roots grown in loose soil, probably as a direct result of the larger mechanical impedance in the cores.
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  • 45
    ISSN: 1573-5036
    Keywords: cassava ; Guelph permeameter ; leaching ; maize ; methylene blue ; rice ; rooting depth ; soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Under high rainfall conditions on acid soils with shallow crop root systems the rate of N leaching is high. A simple model predicts nitrogen uptake efficiency as a function of the amount of rainfall in excess of evapotranspiration, rooting depth and degree to which N leaching is retarded in comparison with water transport. Field observations on acid soils in S.E. Nigeria and S. Sumatera (Indonesia) showed that this model should be amended to include the role of old tree root channels. Crop roots can follow these channels, which are coated with partly decayed organic matter, into the acid subsoil. Measurements of water infiltration with a Guelph permeameter and a methylene blue dye showed that such channels form the major infiltration sites during rainstorms. Implications for nitrogen use efficiency and cropping pattern are discussed.
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  • 46
    ISSN: 1573-5036
    Keywords: aluminium ; calcium ; groundnut ; gypsum ; limestone ; magnesium ; maize ; soil solution ; ultisol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted on an Ultisol in Malaysia to assess changes in soil solution composition and their effects on maize and groundnut yields, resulting from limestone and gypsum application. The results showed that soil solution Ca in the lime treatment remained mainly in the zone of incorporation, but in the gypsum treatment some Ca moved into 15–30 cm zone. Al3+ and AlSO4 + were dominant Al species in the soil solution of nil treatment. Liming decreased Al3+ and AlSO4 +, but increased hydroxy-Al monomer activities. However, gypsum application resulted in an increase of AlSO4 + activity and in a decrease of Al3+ activity. Relative maize and groundnut yields were negatively correlated with Al3+, Al(OH)2+ and Alsum activities. Likewise, relative yields were negatively correlated with Al concentration and the Al concentration ratio and positively correlated with soil solution Mg concentration and Ca/Al ratio.
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  • 47
    ISSN: 1573-5036
    Keywords: cadmium ; copper ; extraction method ; iron ; maize ; manganese ; nickel ; root exudate ; tobacco ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soluble root exudates were collected from three plants (Nicotiana tabacum L., Nicotiana rustica L. and Zea mays L.), grown under axenic and hydroponic conditions, in order to study their metal-solubilizing ability for Cd and other cations (Cu, Fe, Mn, Ni, Zn). Nicotiana spp. and Zea mays L. root exudates differed markedly in C/N ratio, sugars vs. amino acids ratio and organic acids content. Metals from two soils were extracted with either root exudate solutions, containing equal amounts of organic carbon, or distilled water as control. In the presence or absence of root exudates, the solubility of Fe and Mn was much higher than of the four other metals tested. Root exudates increased the solubilities of Mn and Cu, whereas those of Ni and Zn were not affected. Root exudates of Nicotiana spp. enhanced the solubility of Cd. The extent of Cd extraction by root exudates (N. tabacum L. N. rustica L. Zea mays L.) was similar to the order of Cd bioavailability to these three plants when grown on soil. An increase in Cd solubility in the rhizosphere of apical root zones due to root exudates is likely to be an important cause of the relatively high Cd accumulation in Nicotiana spp.
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  • 48
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    Plant and soil 133 (1991), S. 93-100 
    ISSN: 1573-5036
    Keywords: cultivars ; growth inhibition ; hydrogen cyanide ; lettuce ; Pseudomonas fluorescens ; volatile metabolites ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lettuce and wheat cultivars, differing in reaction to root inoculation with plant growth-inhibitory bacteria, were tested for sensitivity to (i) gaseous metabolites produced by deleterious, cyanogenic isolates of Pseudomonas fluorescens, and to (ii) pure cyanide. Reactions were read as shoot and/or root elongation after exposure of seedlings to the volatiles in vitro. Lettuce cultivar Salad Bowl was significantly less sensitive than cv. Montana, both to bacterial volatiles and to pure cyanide, and a similar difference between these cultivars was also obtained in greenhouse experiments where bacteria were inoculated directly on the roots. Cultivar differences were, however, not recorded, either in vitro or in the greenhouse, when the bacteria were grown on a medium which did not support cyanide production. In wheat, a difference in sensitivity to bacteria-produced volatiles was recorded between two cultivars (Drabant and Besso) differing in reaction to bacterial inoculation, but in contrast to lettuce cultivars, the wheat cultivars tested did not react differentially to pure cyanide. The results suggest that in lettuce differential sensitivity to cyanide is one factor behind cultivar differences in reaction to the bacteria tested, even though bacterial metabolites other than cyanide may contribute to the plant growth inhibition recorded. In wheat, however, differential cultivar responses to these bacteria could not be shown to be related to cyanide.
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  • 49
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    Euphytica 59 (1991), S. 1-8 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; root ; combining ability ; correlation ; dry matter ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat root characters which influence vital plant processes have scarcely been explored for their genetic control. This study was conducted to i) examine the diversity of root traits and associated shoot traits in spring wheat (Triticum aestivum L.) cultivars; ii) study the nature of genetic control of selected traits; and iii) examine associations among root and shoot traits. Three experiments were conducted in the greenhouse with plants grown in the vermiculite medium in clear plastic tubes. In the first experiment, 42 spring wheat cultivars were grown for three weeks and measurements were taken on root length, leaf length, root number, leaf number, root dry weight, and top dry weight. In the second study, 15F1's originating from a partial diallel mating of six cultivars along with the parents were evaluated for 4 weeks. The data on root length, leaf length, and root number were subjected to diallel analysis according to Griffing's method 4, fixed model. A third experiment consisted of studying 2 F2 populations with 141 plants per population. In the first study the 42 cultivars exhibited a wide range of variation for all six traits. Diallel analysis revealed significant effects of both general combining ability (GCA) and specific combining ability (SCA) for root length, leaf length, and root number. Parents with high GCA estimates were identified. Significant positive correlation coefficients were found among root and shoot traits. Analysis of F2's for root length indicated quantitative nature of inheritance of root length.
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  • 50
    ISSN: 1573-5060
    Keywords: Breeding ; heritability ; in vitro digestibility ; in vivo digestibility ; maize ; silage maize ; variation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Variation and covariation for agronomic and digestibility traits of silage maize are reported from a compilation of 22 years of experiments with standard sheep. Genotype effects of DOM and DCF were highly significant, even when genotypes were nested in earliness groups or brown-midrib hybrids discarded (Table 2). The genetic variance of crude fiber content was low, but the variance of the DCF was high. The genetic variance of DOM was about 4 times lower than genetic variance of DCF, but broad sense heritability of DOM was higher because of lower residual variance (Table 3). Genetic correlations between grain or crude fiber content and DOM had similar absolute values, 0.65, so each of these two traits was an important but not the unique determinant of silage maize quality. There was no correlation between DCF and grain or crude fiber content. Yield was not related to DOM or DCF within each group of earliness, allowing some quality improvement without agronomic drift (Table 4). Except for late hybrids, most of DOM differences between groups of earliness came from lowering of minimum value, while maximum values were similar. It was the contrary for DCF, with similar minimum values for all groups (Table 5). There was no obvious correlation between year of registration of hybrids and DOM or DCF, but extra new variation seemed obtained only for low values (Figs 1, 2; Table 6). IVDOM according to the APC process was a poor predictor of DOM, especially when brown-midrib hybrids and earliness effects were discarded; but because heritability of this trait was similar to DOM heritability, such enzymatic processes could probably be used to avoid drift towards poor DOM with hybrids bred for higher stalk strength.
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  • 51
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum compactum ; club wheat ; Triticum spelta ; spelt wheat ; classification ; genetic diversity ; landraces ; cultivars ; USA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In 1922 Clark, Martin and Ball published descriptions of 207 hexaploid wheat landraces and improved cultivars, collected in the USA: 189 bread wheat accessions (Triticum aestivum), 24 club wheats (T. compactum) and four spelt wheats (T. spelta). After omitting 24 bread wheat accessions with identical descriptions as other accessions, the descriptions were used here to classify the remaining 183 accessions into five clusters on the basis of 10 selected characters. Clusters 1 and 3 include mainly accessions introduced from North and West Europe. These accessions must derive from the European Zeeuwse and Gelderse landrace groups. Hence most of the accessions belonging to these two clusters belong to the North and West European heritage. Cluster 2 includes most of the accesions introduced from Australia and Canada, or belonging to the club wheats. Most accessions from the USSR1 are included in cluster 4. Here we also find Turkey and related landraces. All but one pubescent accessions are included in cluster 5. The wide variation of US wheats, described in 1922, forms the basis of the Foundation Germplasm wheats as defined by Cox (1991). Apparently no hard white winter wheat landraces and cultivars were described.
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  • 52
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; hybridization ; pollen-tube-growth ; wide-crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five- to seven-day-old ovaries from Triticum aestivum vars Hope (non-crossable), Chinese Spring (CS, crossable) and the chromosomal substitution line Chinese Spring/Hope 5B (CS/H5B) were used to test the hypothesis that an ovarian substance inhibits rye (Secale cereale var Wrens) pollen tube growth. In vitro assays on a semi-solid medium demonstrated that a soluble, dialyzed lysate from Hope or CS/H5B ovaries inhibited rye pollen tube elongation significantly more than a similar lysate from CS ovaries (26%, 13% and 5% respectively compared to controls). When only the 20 longest tubes from each sample were included in the analysis the relationship was maintained (33%, 22% and 15% for Hope, CS/H5B and CS respectively). Heating the Hope lysate at 100° C for 10 min reduced the inhibition from 26% to 14% for all pollen tubes measured (n = 51 per sample) and from 33% to 20% when only the 20 longest pollen tubes per sample were analyzed. Isoelectric focusing followed by sodium dodecyl sulfate polyacrylamide electrophoresis (IEF/SDS-PAGE) and non-equilibrium pH gradient electrophoresis (NEPHGE) revealed several differences in the polypeptide profiles of soluble lysates from vars Hope, CS and CS/H5B. While two striking differences were observed, a 50 kilodalton (kD) polypeptide with an isoelectric point (pI) of approximately 8.5 and 100 kD polypeptide (pI∼4) from CS lysates which were considerably reduced in lysates of Hope and CS/H5B, none were obviously associated with the Hope 5B chromosome and therefore cross-incompatibility.
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  • 53
    ISSN: 1573-5060
    Keywords: bread wheat ; Erysiphe graminis ; powdery mildew ; Triticum aestivum ; Triticum dicoccoides ; wild emmer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A new source of resistance to wheat powdery mildew caused by Erysiphe graminis has been transferred to hexaploid bread wheat, Triticum aestivum, from the wild tetraploid wheat, Triticum dicoccoides. The donor was crossed to bread wheat and the pentaploid progeny was then self-pollinated. Plants having a near stable hexaploid chromosome complement were selected in the F3 progeny and topcrossing and backcrossing of these to a second wheat cultivar to improve the phenotype was undertaken. Monosomic analysis of early backcross lines showed the transferred gene to be located on chromosome 4A. The gene has been designated Pm16.
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  • 54
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    Euphytica 53 (1991), S. 137-141 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; crossability ; Himalayas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crossability of 62 bread wheat accessions (14 landraces from Himachal Pradesh and 48 others) was examined with rye. The 3 rye cultivars did not differ in their relative crossability with 4 of the wheat accessions studied. On the other hand, the wheat cultivars differed greatly among themselves in their crossability with rye. Most of the wheat cultivars showed poor (〈10%) crossability. Two of the 14 landraces from Himachal Pradesh were found to be free from the crossability inhibitors as they showed very high (〉50%) crossability, whereas none of the other 48 cultivars studied was so.
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  • 55
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Aegilops sharonensis ; male sterility ; preferential transmission ; hybrid wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat plants nullisomic for chromosome 4B are male sterile due to the absence of the male fertility gene Ms1. However, plants in which chromosome 4B has been substituted by the preferentially transmitted chromosome 4S1 of Ae. sharonensis are male fertile due to the compensating effect of Ms4 on the alien chromosome. This substitution line has been mutated and three recessive mutation of Ms4 have been selected. Plants homozygous for these mutations are male sterile. The implication of these mutations for hybrid wheat production is discussed.
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  • 56
    ISSN: 1573-5060
    Keywords: boron ; inheritance ; tolerance ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic control of tolerance of wheat to high concentrations of soil boron was studied for five genotypes. Each genotype represented one of five categories of response to high levels of boron, ranging from very sensitive to tolerant. Tolerance to boron was expressed as a partially dominant character, although the response of an F1 hybrid, relative to the parents, varied with the level of boron applied. The F1 hybrids responded similarly to the more tolerant parent at low B treatments and intermediate to the parents at higher treatments. Ratios consistent with monogenic segregation were observed for the F2 and F3 generations for the combinations (WI*MMC) × Kenya Farmer, Warigal × (WI*MMC) and Halberd × Warigal. The three genes, Bo1, Bo2 and Bo3, while transgressive segregation between two tolerant genotypes, G61450 and Halberd, suggested a fourth locus controlling tolerance to boron.
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  • 57
    ISSN: 1573-5060
    Keywords: Canada ; genotypic differences ; manganese toxicity ; manganese tolerance ; origin ; pedigree ; screening ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Breeding wheat (Triticum aestivum L.) for tolerance to manganese (Mn) might be in some cases more feasible and economical than use of soil amendments. As part of research on the heritability of Mn tolerance, a study on the level of Mn tolerance in Canadian wheat cultivars and its probable origin was accomplished by analysis of cultivar pedigrees and drawing phylogenetic maps to discern filial relationships. Cultivar tolerance to Mn was determined by relative root weight (RRW) in solution culture in the presence of 500 μM Mn. A total of 91 cultivars were screened, 76 of which were Canadian. These data, together with data from another 28 cultivars reported in the literature, were used to draw two pedigree maps, a map for Canadian cultivars only, and a map for the Mn-tolerant Canadian cultivars Norquay and Laura. Results indicated a range of tolerance to Mn among Canadian cultivars. Manganese tolerance, found in either Canadian or foreign germplasm, and of either recent or older selection or origin, seems to have originated from land races from Rio Grande do Sul, the southernmost state of Brazil. Tolerance may have been introduced into Canadian germplasm directly by the use of Brazilian cultivars as parents, or indirectly by the introduction of Mexican germplasm with Brazilian parentages. This information will help the plant breeder to develop plant breeding systems, and may also help in the study of the mechanisms for Mn tolerance in wheat.
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  • 58
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Diuraphis noxia ; Russian wheat aphid ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Russian wheat aphid (Diuraphis noxia, Mordvilko) (RWA) is responsible for significant economic damage to cereal crops in arid and semi-arid environments. In this research 20 red winter wheats originating from Iran were evaluated for resistance to RWA. Leaf rolling, leaf folding, and leaf chlorosis were measured using 0 to 3 scales. An overall mean damage score was calculated as the average of the three measured damage symptoms. Plants from seven central Asian accessions (PI222666, PI222668, PI225226, PI225267, PI225271, PI243630, and PI243642) had mean damage scores significantly lower (p 〈 0.001) than ‘Stephens’ wheat (RWA susceptible) and not significantly different from ‘Border’ oat (RWA resistant). These results are consistent with previous studies which found a high frequency of resistant wheats collected from the central Asian region.
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  • 59
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    Euphytica 58 (1991), S. 57-63 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; cold hardiness ; cultivar x tillage interaction ; tillage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Conservation tillage in the Great Plains of North America has increased in recent years, in part, to control soil erosion, increase soil water storage and reduce production costs. No-till production is recommended for winter wheat (Triticum aestivum L.) in northern latitudes for the purpose of trapping snow and protecting the crop from freezing temperatures. A 5 year field study was conducted at four North Dakota locations from 1984–85 through 1988–89 to determine if significant cultivar x tillage interactions existed for grain yield of winter wheat. Fourteen hard red winter wheat cultivars were planted in both conventional-till and no-till systems. Significant cultivar x tillage interactions were obtained for grain yield from both a weighted analysis across all environments and from individual analyses of 12 of the 16 environments in which wheat survived under both tillage systems (P〈0.05). Regression of cultivar yield on mean environment yield produced b values that varied little between conventional till and no-till for 10 of the 14 cultivars. The top five yielding cultivars were the same for both tillage systems and had b values 〉 1.0. Although the cultivar x tillage interaction may be a result of differential winter survival rather than tillage per se, this study suggests that selection for winter wheats with superior yield performance can be conducted under either no-till or conventional-till systems.
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  • 60
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    Euphytica 58 (1991), S. 171-181 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four spring wheat (Triticum aestivum L.) varieties differing in origin and reaction in the seedling stage to pathotype CDL-6 (extant in California) were intercrossed and examined in greenhouse conditions in F1, F2, and F3 generations. Digenic and transgressive segregation was found in all crosses. The four varieties each had infection types (1 immune, 9 susceptible) and putative resistance genes as follows: ‘Anza’, IT 7, YrA; ‘Glennson 81’, IT 2, Yr9; ‘Yecora Rojo’, IT 6, YrC; and ‘Ollanta’, IT 4–6, YrL. ‘Anza’ was classified as susceptible, ‘Yecora Rojo’ and ‘Ollanta’ as intermediate in seedling resistance, and ‘Glennson 81’ as resistant in the seedling stage.
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  • 61
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; day-length insensitivity ; semi-dwarfness ; tissue culture response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of genes affecting the ‘plant growth habit’ in wheat (Rht8 and Ppd1) and rye (ct1 and ct2) on tissue culture response was studied using immature embryos. Whereas the semi-dwarfing gene Rht8 seems to promote only a minor effect, the day-length sensitive allele ppd1 determined a major increase in callus growth and regeneration ability. With regards to their tissue culture efficiency, the four alleles studied could be ranked as follows: ppd1〉Rht8〉rht8〉Ppd1. In contrast to wheat, the GA insensitive semi-dwarfing genes of rye (ct1 and ct2) appear not to influence in vitro response.
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  • 62
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    Plant and soil 137 (1991), S. 191-199 
    ISSN: 1573-5036
    Keywords: durixerollic calciorthid soil ; evapotranspiration ; plant water relations ; silicon transport soil ; temperature ; Triticum aestivum ; uptake ; water balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Silicon (Si) content in cereal plants and soil-Si solubility may be used to estimate transpiration, assuming passive Si uptake. The hypothesis for passive-Si uptake by the transpiration stream was tested in wheat (Triticum aestivum cv. Stephens) grown on the irrigated Portneuf silt loam soil (Durixerollic calciorthid) near Twin Falls, Idaho. Treatments consisted of 5 levels of plant-available soil water ranging from 244 to 776 mm provided primarily by a line-source sprinkler irrigation system. Evapotranspiration was determined by the water-balance method and water uptake was calculated from evapotranspiration, shading, and duration of wet-surface soil. Water extraction occurred from the 0 to 150-cm zone in which equilibrium Si solubility (20°C) was 15 mg Si L−1 in the Ap and Bk (0–58 cm depth) and 23 mg Si L−1 in the Bkq (58–165 cm depth). At plant maturity, total Si uptake ranged from 10 to 32 g m−2, above-ground dry matter from 1200 to 2100 g m−2 and transpiration from 227 to 546 kg m−2. Silicon uptake was correlated with transpiration (Siup=−07+06T, r2=0.85) and dry matter yield with evapotranspiration (Y=119+303ET, r2=0.96). Actual Si uptake was 2.4 to 4.7 times that accounted for by passive uptake, supporting designation of wheat as a Si accumulator. The ratio of Si uptake to water uptake increased with soil moisture. The confirmation of active Si uptake precludes using Si uptake to estimate water use by wheat.
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  • 63
    ISSN: 1573-5036
    Keywords: alley cropping ; beans ; Erythrina ; Gliricidia ; humid tropics ; isotopically exchangeable P ; maize ; mulching ; phosphorus cycle ; P desorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phosphorus availability was measured in soils under five cropping systems: alley cropping with Erythrina poeppigiana, alley cropping with Gliricidia sepium, sole cropping with Erythrina poeppigiana mulch applied, sole cropping with Gliricidia sepium mulch applied, sole cropping with no mulch. The following parameters were measured: 1) plant-available soil P assessed by P uptake of maize and bean bioassay plants; 2) phosphate desorbable by anion exchange resin; 3) adsorption of added P into isotopically exchangeable and non-exchangeable pools. In the bioassay, P uptake of beans declined in the order: mulched sole-cropped〉unmulched sole-cropped〉alley-cropped soils. For maize the relative uptake was: mulched sole-cropped〉unmulched sole-cropped = alley-cropped soils. These results suggest trees had not incorporated a significant quantity of P into the system after seven years and, probably, there was a decrease in available soil P due to the sequestration of P in the tree biomass. Potentially resin-desorbable P was higher in alley-cropped and mulched sole-cropped soils than in unmulched sole-cropped soils. The adsorption and desorption of added P into and from exchangeable and non-exchangeable pools did not differ between alley-cropped and unmulched sole-cropped soils. Crop yield and crop N, P and K uptake were all higher in the alley crops than in the unmulched sole crop. The supply of P to the crop under alley cropping seems to be dependent on P cycled and released from the mulch. The P cycle in alley cropping appears to be self-sustaining at least under conditions of moderate P fertiliser input.
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  • 64
    ISSN: 1573-5036
    Keywords: fertilizer trials ; Kenya ; maize ; Striga hermonthica ; sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The adverse effect of the parasitic weed Striga hermonthica on yield of maize was studied in a fertilizer trial in Southwestern Kenya. In two years of experimentation (1987 and 1988), the weed had a highly significant, negative impact on maize yields. The spotty incidence of Striga disturbed the fertilizer trials. Inclusion of the degree of Striga infestation in a regression model caused an increase in the fraction of experimental variation that could be explained by the model. Several methods were tested to combat Striga. Hand-pulling reduced Striga incidence and increased grain yields during the following growing season. No clear effect was obtained from the trap crop sunflower, although such an effect may have been concealed by the success of hand-pulling. Application of mineral fertilizers or farmyard manure did not significantly reduce Striga infestation.
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  • 65
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    Plant and soil 138 (1991), S. 139-142 
    ISSN: 1573-5036
    Keywords: maize ; manganese deficiency ; pollen viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Maize (Zea mays L. cv. G2) was grown with 0.55 mg L−1 (sufficient), or 0.0055 mg L−1 (deficient) manganese in sand. Manganese-deficient plants developed visible deficiency symptoms and showed poor tasseling and delayed anther development. Compared to Mn-sufficient plants, Mn-deficient plants produced fewer and smaller pollen grains with reduced cytoplasmic contents. Manganese deficiency reduced in vitro germination of pollen grains significantly. Ovule fertility was not significantly affected by Mn. But in Mn-deficient plants seed-setting and development was reduced significantly.
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  • 66
    ISSN: 1573-5036
    Keywords: aluminium tolerance ; cation-anion balance ; phosphate translocation ; split-root ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seedlings of two cultivars of wheat (Triticum aestivum L.) differing in tolerance to aluminium (Al) were grown using a split-root sand/soil culture technique. Each culture tube was divided horizontally into a surface (0–150 mm) compartment and a subsurface (150–250 mm) compartment separated by a root-permeable paraffin wax barrier. Thus phosphorus (P) supplied to surface roots could not percolate or diffuse into the soil in the subsurface compartment. The soil in the subsurface compartment was divided into ‘rhizosphere’ and ‘non-rhizosphere’ zones using a porous (5 μm) membrane. Root growth of both cultivars into the subsurface zone was enhanced by increased P supply to surface roots, but did not conform to known relationships between root growth and soil pH, extractable-Al, or pH, Al or P concentrations in soil solution. Concentrations of Al in soil solution in the rhizosphere were greater than those in solution in the bulk soil. Concentrations of Al reactive with pyrocatechol violet (30s-RRAI) in the rhizosphere soil solution were generally greater than those in non-rhizosphere soil. With the Al-sensitive cultivar, root dry weight and length increased as concentrations of RRAl in the rhizosphere soil solution increased. Increased concentrations of Al in rhizosphere soil solutions were not related to the presence of organic ligands in solution. The effect of P in promoting root penetration into the acidic subsurface stratum was not related to differential attainment of maturity by the plant shoots, but appeared to be related to the effect of P in enhancing the rate of root growth. Thus, suboptimal supply of P to the surface roots of a plant, even at levels sufficient to preclude development of nutritional (P) stress symptoms, may seriously reduce tolerance to Al, and hence diminish the ability of roots to penetrate into acidic subsoils.
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  • 67
    ISSN: 1573-5036
    Keywords: aglycone ; allelopathy ; arabinose ; bioassay ; Bromus secalinus ; dormancy rating ; Medicago sativa ; saponins ; thin-layer chromatography (TLC) ; Trichoderma viride ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat, cheat and Trichoderma viride bioassays were used to establish the relationship between the content of biologically active saponins in the roots and the degree of winter dormancy and/or time of six cultivars of alfalfa over the period January to August, 1988. Wheat and cheat bioassay results indicated no significant difforences among cultivars, whereas T. viride was inhibited most by extracts of roots collected during months with high rainfall and rapid growth. Cheast seedling roots were inhibited 8–10% more than those of wheat seedling roots indicating that alfalfa root saponins were more effective as allelopathic compounds in preventing growth of cheat than that of wheat alone. An average of 14 different saponins per cultivar were separated by thin-layer chromatograms. Saponins, and the aglycones produced by acid hydrolysis of the May samples, were separated by thin-layer chromatography. The conclusion is that the amount, structure, and type of saponins present in alfalfa roots vary with time.
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  • 68
    ISSN: 1573-5036
    Keywords: nitrate reductase ; nitrite reductase ; organic nitrogen ; tritordeum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tritordeum is a fertile amphiploid derived from durum wheat (Triticum turgidum L. conv. durum) × a wild barley (Hordeum chilense Roem. et Schultz.). The organic nitrogen content of tritordeum grain (34 mg g-1 DW) was significantly higher than that of its wheat parent (25 mg g-1 DW). Leaf and root nitrogen content became higher in tritordeum than in wheat after four weeks of growth, independently of the nitrogen source (either NO3 - or NH4 +). Under NO3 - nutrition, tritordeum generally exhibited higher levels of nitrate reductase (NR) activity than wheat. Nitrite reductase (NiR) levels were however lower in tritordeum than in its wheat parent. In NH4 +-grown plants, both NR and NiR activities progressively decreased in the two species, becoming imperceptible after 3 to 5 weeks of growth. Results indicate that, in addition to a higher rate of NO3 - reduction, other physiological factors must be responsible for the greater accumulation of organic nitrogen in tritordeum grain.
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  • 69
    ISSN: 1573-5036
    Keywords: growth promotion ; sterile red fungus ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Enhancement of shoot and root growth of wheat seedlings by a sterile red fungus was evident even when sufficient nutrients were supplied and density of plants per pot was increased (from 15 to 30 plants per pot of 400 g soil). It is likely that production of hormonal-type substances by these fungi may be involved in the phenomenon observed.
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  • 70
    ISSN: 1573-5036
    Keywords: chlorophyll ; genotypic differences ; leaf elongation rate ; Mn toxicity ; Mn tolerance ; screening ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract After aluminum toxicity, manganese (Mn) toxicity is probably the second most important growth limiting factor in acid soils. The purpose of this study was to determine the feasibility of using chlorophyll content and leaf elongation rate (LER) for regrowth of Mn stressed seedlings as a rapid seedling based screening bioassay for Mn tolerance in segregating populations of wheat (Triticum aestivum L.). In one experiment, chlorophyll was determined for the cultivars Norquay (Mn-tolerant) and Columbus (Mn-sensitive) subjected to twelve Mn levels (2 to 2000 μM) in nutrient solutions. As Mn concentration increased, chlorophyll ‘a’ and ‘b’ contents of the Mn-tolerant cultivar decreased up to 9%, while in the Mn-sensitive cultivar it was reduced by as much as 43%. The chlorophyll ‘a/b’ ratio did not differ among Mn concentrations for either cultivar. In a second experiment, chlorophyll content and LER for regrowth of Mn stressed seedlings (1000 μM) was determined for Columbus and Katepwa (Mn-sensitive), Oslo (Mn-intermediate), and Norquay and Laura (Mn-tolerant). Manganese tolerance as assayed by chlorophyll ‘a’ and ‘b’ and LER was significantly correlated with Mn tolerance as assayed by the relative root weight methodology (RRW). Thus, chlorophyll content of Mn-stressed seedlings and LER of seedling regrowth appear to be suitable techniques for screening unreplicated selections of segregating populations for tolerance to Mn.
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  • 71
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    Plant and soil 134 (1991), S. 127-135 
    ISSN: 1573-5036
    Keywords: aluminium and acid saturation ; exchangeable aluminium and acidity ; lime requirement indices ; maize ; relative crop yields ; seasonal effects ; soil pH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Due, in part, to the relative paucity of published comparisons based on field generated data, there is still poor agreement regarding the relative merits of lime requirement indices based on exchangeable Al and those based on pH. The objective of this study was to compare such indices using results obtained from long-term field experiments. Data were obtained over 22 site-years from lime trials conducted on clay (Typic Haplorthox) and sandy loam (Plinthic Paleudult) soils differing widely in organic carbon content. Relative maize (Zea mays L.) yields were used to compare the prognostic value of soil pH with indices obtained using exchangeable Al and exchangeable acidity (Al+H). Both within and across soils, pH proved to be markedly inferior to Al based indices. Exchangeable acid saturation of the effective cation exchange capacity, a readily obtained and popular index of lime requirement in some countries, proved as effective as less easily acquired indices based on exchangeable Al per se. The findings reported are consistent with those of many glasshouse studies and support the viewpoint that indices based on Al or acid saturation should replace pH as a measure of lime requirement.
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  • 72
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    Plant and soil 134 (1991), S. 277-280 
    ISSN: 1573-5036
    Keywords: bleeding rate ; maize ; root pruning ; root-study methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Current methods of studying roots are either labour intensive or require expensive equipment. In 1986 and 1987 root pruning treatments were given to maize plants grown indoors. In both years the amount of xylem exudate collected from plant stumps shortly after root pruning increased with the dry weight of roots attached to the plant. The measurement of xylem exudation is presented as an alternative for conventional laborious root-study methods.
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  • 73
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    Plant and soil 135 (1991), S. 213-221 
    ISSN: 1573-5036
    Keywords: maize ; plant growth-regulating substances ; plant hormones ; substrate dependend-cytokinin production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Glasshouse experiments were conducted to evaluate the influence of pretested cytokinin precursors, adenine (ADE) and isopentyl alcohol (IA), and a cytokinin producing bacterium, Azotobacter chroococcum added to soil, on the vegetative growth of maize (Zea mays L.). The combination of 2.0 mg ADE kg-1 soil, 13 mg IA kg-1 soil, plus an inoculum of A. chroococcum was the most effective in enhancing the vegetative growth of maize compared with the application of ADE plus IA, ADE plus A. chroococcum, or ADE, IA or A. chroococcum alone. The dry weight of root and shoot tissues was increased up to 5.57- and 5.01-fold, respectively, in comparison to the controls; however, the root/shoot ratios were similar. The increases in shoot height, internodal distance, stem and leaf width over the controls under the optimum treatment were: 2.07-, 2.81-, 1.46-, and 2.11-fold, respectively. The improvement in plant yield was primarily attributed to A. chroococcum production of cytokinins in the rhizosphere.
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  • 74
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    Plant and soil 136 (1991), S. 183-193 
    ISSN: 1573-5036
    Keywords: diagnosis ; distribution ; magnesium deficiency ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aims of this study were to describe the distribution of magnesium (Mg) and its retranslocation within wheat, in order to develop diagnostic procedures for Mg deficiency. Plants were grown in solution culture with both constant supply (0, 5, 10, 20, 40, 80, 160 μMMg) and discontinued supply (40 μM and 160 μM decreased to nil). Magnesium was depleted from old leaves when Mg supply to the roots was halted. However, initial deficiency symptoms occurred on young leaves under constant but inadequate supply, contrasting with previous reports. Magnesium concentrations were also lower in young leaves compared to old leaves. Symptoms of yellowing and necrosis occurred if the leaf tissue contained 〈1194 μgg−1, irrespective of leaf age. The minimum Mg concentration in whole shoots associated with maximum shoot weight was 932 μgg−1; for the youngest emerged blade (YEB) it was 861 μgg−1. Symptoms were apparent on the young leaf before a reduction in shoot weight was measurable. The concentration of Mg in the YEB and whole shoot were better related to solution Mg concentration than was the Mg concentration in the old leaf.
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  • 75
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    Plant and soil 136 (1991), S. 205-213 
    ISSN: 1573-5036
    Keywords: barley ; carbon ; 14C ; exudates ; partitioning ; respiration ; rhizodeposition ; root ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (cv. Gutha) and barley (cv. O'Connor) were grown as field crops on a shallow duplex soil (sand over clay) in Western Australia with their root systems contained within pvc columns. At four stages during growth, the shoots were pulse-labelled for 1.5h with14CO2; immediately prior to labelling, the soil was isolated from the shoot atmosphere by pvc sheets. After labelling, the soil atmosphere was pumped through NaOH to trap respired CO2 and after 2.5, 5, 7.5 and 24 h from the start of labelling, columns were destructively sampled to recover14C from the roots, soil and shoot. Both species showed similar patterns of14C distribution and changes in distribution through the growing season. During early tillering, 15–25% of the14C recovered after 24 h had been respired by the roots and rhizosphere, 17–27% was retained in the roots, 0.4–1.8% was recovered as water-soluble14C in the soil and the remainder (45–67%) was present in the shoot. These percentages changed during growth so that during grain filling only 2–3% of the14C recovered after 24 h was as respired CO2, 2–6% was in the roots, 0.2% was in the soil and over 90% was in the shoot. The distribution of14C in components of the soil-plant system changed during the 24 h after labelling with the most rapid changes occurring generally during the first 7.5 h after labelling. Using growth measurements from adjacent plots, the amounts of C added to the soil were estimated for the whole season. Carbon input to the soil was about 48 gC m−2 for wheat and 58 gC m−2 for barley; the crops produced total shoot dry matter of 494 (wheat) and 735 g m−2 (barley). Of the C input to the soil, 27.8% (wheat) and 40.3% (barley) was as respired C and only 3.3 (wheat) and 4.1% (barley) was collected as exudate (water-soluble material).
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  • 76
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    Plant and soil 138 (1991), S. 189-194 
    ISSN: 1573-5036
    Keywords: aggregate size ; carbon allocation ; maize ; shoot-root ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Shoot dry mass and leaf area of 16-d old maize plants decreased as soil aggregate size in greenhouse pots increased in diameter from 0.075–0.5 to 4–8 mm. Root length was also much greater on the finer aggregate beds, due primarily to increased growth of second-order laterals. In a subsequent experiment in which shoot dry matter again decreased with increasing aggregate size, it was found that a similar change in root morphology as noted in experiment I resulted in increased root dry mass as aggregate size increased. The associated change in shoot-root ratio was significant eight days after emergence. This change was due to a change in allocation of fixed carbon rather than allocation of seed reserves. Neither transpiration rate per unit leaf area, nor net assimilation rate were affected by aggregate size. Likewise nutrition could not account for the differences in shoot or root growth.
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  • 77
    ISSN: 1573-5060
    Keywords: Zea mays ; maize ; genetic variation ; nitrate accumulation ; N use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The objective of this research was to obtain information about expression of genes controlling N concentration in the lower stalk internodes of maize at silking stages and in two other stages during the grain filling period. The inheritance of nitrate-N concentration, total N concentration, and the nitrate-N/total N ratio in the lower stalk internodes was measured in an eight-line diallel cross experiment and in an experiment involving segregating generations. The results show that the parameters examined, particularly nitrate-N concentration, are genetically controlled and that maize plants differ in this respect. Moreover, the magnitude of the general combining ability effects in relation to the size of specific combining ability obtained from the diallel analysis and the mean squares of the analysis of variance of generation means indicated additive heritability of nitrate-N concentration and other N-related traits in maize stalks. These findings suggest to us that a cyclic selection program in maize should be effective in modifying the level of N-related traits concentration in the stalks.
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  • 78
    ISSN: 1573-5060
    Keywords: Triticum ; wheat ; sprouting tolerance ; dormancy ; variation ; heritability ; correlation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Germination of wheat (Triticum spp) kernels prior to harvest reduces the economic value of grain. Losses attributable to pre-harvest sprouting could be reduced by developing sprouting tolerant (ST) cultivars if heritable variation exists for this trait. Objectives of this study were to compare various assays that measure ST, seed dormancy (SD), and alpha-amylase activity, and to divide the total variation for these traits into parts relating to genetic differences, genotype-year interaction, and residual variation. Twenty-six hexaploid wheat genotypes (Triticum aestivum L.) (eight red-kernelled and eighteen white-kernelled), and three white-kernelled tetraploid wheat genotypes (T. turgidum L. var. durum) were subjected to various assays that measure ST, SD, and alpha-amylase production using artificial wetting treatments. Genotype effects accounted for 44% to 90% of the phenotypic variation. The genetic component of variation, when expressed as a proportion of the total variation, was 2 to 6 times greater than the proportion attributable to error. Heritability expressed on a genotype mean basis was highly significant for all variables and ranged from 0.59 to 0.93. The phenotypic correlation for any ST or SD trait or alpha-amylase activity measured on samples collected at two dates (T1 and T2 = T1+14 days) was highly significant and positive. The phenotypic correlation matrix among assays performed on the unthreshed spikes, germination tests and alpha-amylase activity at both T1 and T2 were positive and highly significant. Increased ST is an attainable objective, and direct selection for ST using an artificial wetting treatment is an appropriate breeding strategy.
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  • 79
    ISSN: 1573-5060
    Keywords: Zea mays ; maize ; prediction ability ; discrimination ability ; yield ; digestibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The identification of environments suitable for selection should enable plant breeders to test reliably a larger number of genotypes with given resources. This research was undertaken to evaluate discrimination ability (DA) and prediction ability (PA) of eight environments involving two locations. Eckartsweier (Eck) 1 and Voelkenrode (Voe); 2 yer. 1984 (84) and 1985 (85); and two harvests, silage harvest (I) and later (II). It was based on dry matter yield of plant (PDMY), ear (EDMY) and stover (SDMY) and on in vitro digestible organic matter (IVDOM), neutral detergent fibre (NDF), acid detergent fibre (ADF) and acid detergent lignin (ADL) of stover of 12 inbred lines and their 66 diallel crosses in maize (Zea mays L.). Linear regression coefficient of the performance of genotypes in an environment on that averaged across all environments and its degree of determination were used as measures of the DA and PA of environments, respectively. In hybrids, the DA of environments differed significantly for all traits except SDMY and ADL. Environment Eck851 showed better PA (≥0.63) than other environments for PDMY, EDMY, IVDOM, NDF, and ADF. Among the 12 two-environment combinations. EckI was a better predictor for PDMY, EDMY, IVDOM and NDF (PA≥0.80). The more productive environments showed better DA and PA for PDMY and FDMY than less productive ones. For IVDOM and NDF the first harvest provided better discrimination and prediction than the second harvest. In inbred lines the DA of environments differed significantly for EDMY only. In the present study, PA and DA seemed to be functions of the environments, PA was improved by combining two environments, and some environments seemed to be suitable for preliminary selection of genotypes for such diverse traits as yield and digestibility.
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  • 80
    ISSN: 1573-5060
    Keywords: Triucum aestivum ; wheat ; storage protein ; bread-making quality ; N-banding PA ; subtilisin inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four methods for detecting 1BL. 1RS translocations in bread wheat have been compared winter wheat cultivars: N-banding of mitotic metaphase chromosomes, sodium lactate electrophoresis at pH 3.1, sodium dodecyl sulphate-polyacrylamide gel electrophorests under conditions, and a recently characterised protein, subtilisin inhibitor, separated by isoelectric N-banding was much the most labour intensive method, and, of the three electrophoretic recommend the use of subtilisin inhibitor, which is at least as easy to interpret as the other is often faster for screening purposes. The sources of the 1BL. 1RS translocation in this matenal Avrora. Kavkaz and Skorospelka 35, which have been extensively used as parents in breeding programmes. Out of 59 cultivars that include a line carrying the 1BL. 1RS pedigree. 23 of them did not carry the translocation; thus the effect on plant phenotype of insufficient to guarantee its selection during breeding.
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  • 81
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; drought resistance ; yield ; selection ; grain filling ; kernel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Post-anthesis chemical desiccation of wheat (Triticum aestivum L.) plants in the field eliminates transtent photosynthesis by killing all green tissues, thus revealing the plant's capacity for grain filling from stored stem reserves, as the case is for post-anthesis stress such as drought or leaf diseases. This study was conducted to investigate whether mass selection for large kernels under chemical desiccation would lead to the improve ment of grain filling in the absence of transient photosynthesis. Six crosses of common spring wheat were subjected to three cycles of mass selection from F2 through F1 when selection was performed for large kernels by sieving grains from plants that were erther chemically desiccated after anthesis, or not (controls). The resulting 36 bulks (six crosses by three selection cycles by two selection environments) were compared with their respective F2 base populations, when tested with and without chemical desiccation. Selection for large kernels under potential conditions (without chemical desiccation) did not improve kernel weight under potnetial conditions, evidently because these materials were lacking in genetic variation for kernel weight under potential conditions. In four of the crosses, 3rd cycle selection for large kernels under potential conditions decreased kernel weight under chemical desiccation. On the other hand, selection for large kernels under chemical desiccation was effective in improving kernel weight and test weight under chemical desiccation, depending on the cross and the selection cycle, with no genetic shift in mean days to heading or mean plant height. Selection for large kernels under chemical desiccation was also effective in some cases in increasing kernel weight under potential conditions. The results are interpreted to show that selection under potential conditions and under chemical desiccation operate on two different sources for grain filling, namely transient photosynthesis and stem reserve utilization, respectively. In order to expose genetic variability for stem reserve utilization to selection pressure, transient photosynthesis must be eliminated, as done by chemical desiccation in this study.
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  • 82
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    Euphytica 54 (1991), S. 285-295 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; gliadins ; APAGE ; multiple allelism ; variation ; Yugoslavia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The complete gliadin allele composition of 57 Yugoslav common winter wheat cultivars was studied. Large differences were found in gliadin genotypes among cultivars bred at different Yugoslav breeding centres as well as between early and recent wheats bred in the Novi Sad breeding centre. Yugoslav wheats have limited variation in gliadin alleles, of which Gli-A1a, Gli-B1b, Gli-B1l, Gli-D1b, Gli-A2e, Gli-B2b, Gli-D2a are shown to be the most frequent. Examples of favoured alleles to new cultivars are described. 40% of the studied wheat cultivars have natural biotypes differing in gliadin allele composition. The frequency of cultivars with the biotypes differs greatly among breeding centres.
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  • 83
    ISSN: 1573-5060
    Keywords: adult plant resistance ; seedling resistance ; Septoria nodorum ; septoria nodorum blotch ; X Triticosecale ; triticale ; Triticum aestivum ; wheat ; T. spelta ; spelt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary On average, the cereal species studied were susceptible to septoria nodorum blotch (SNB), except for spring triticale on leaf and head and winter titicale on leaf, that appeared to be significantly more resistant, than the other ones. In all three species the SNB response of the adult plants was to a limited extent only predicted by the reaction on first leaf seedlings. In most cases it was impossible to predict the response to SNB of adult plants on the basis of seedling reaction. Correlations between the adult plant stage and the seedling stage, or detached seedling leaves, appears not to be sufficient for use in practical breeding work. A reversal of reaction to SNB was even found between the above growth stages in studied spring and winter wheat varieties.
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  • 84
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    Euphytica 55 (1991), S. 229-234 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; harvest index ; diallel analysis ; genotypic and phenotypic correlations ; assimilate partitioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cereal breeders have used harvest index (HI) as a selection criterion in segregating generations to identify physiologically superior lines with improved partitioning of total assimilate into grain. Information on combining ability for HI of the hard red winter wheat (Triticum aestivum L.) cultivars of the Southern Great Plains is not available. A study was undertaken to examine HI of seven genetically diverse winter wheat parents, evaluate their general combining ability (GCA) and specific combining ability (SCA) effects, and study correlations of HI with several agronomic traits. The seven parents were crossed in a half-diallel mating design to produce 21 crosses. The F1's, their F2 progenies, and the parents were evaluated in replicated field tests at Stillwater and at Lahoma, OK. The combining ability analysis was performed using Griffing's Method 4, Model 1. The results showed significant variation among parents for HI. The GCA and the SCA effects were mostly inconsistent between generations and between environments. However, parents with consistently high HI and positive GCA estimates were identified. The progeny with high HI mostly resulted from parents with high GCA estimates. The correlations between HI and agronomic traits indicated that improvement in HI should also result in high grain yield, early maturity, and short plant height.
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  • 85
    ISSN: 1573-5060
    Keywords: HMW glutenin subunits ; bread-making quality ; Triticum aestivum ; wheat ; epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relation has been studied between the high molecular weight glutenin (HMWg) subunit alleles and the bread-making quality of 226 lines of winter wheat (T. aestivum L.), grown in The Netherlands. The lines represented a wide range of genetic backgrounds, and had not been selected for quality, in contrast to the established varieties used by other authors. The variation in HMWg subunit genotypes accounted for about 20% of the total variation in loaf volume among the lines. Most important was the allelic variation at the Glu-D1 locus. The Glu-D1 allele encoding the subunits 5+10 was superior to its allelic counterpart, encoding 2+12. The difference in average of loaf volume between groups of lines containing 5+10 or 2+12 was negatively related with protein content of the flours. When protein content was below 9.2%, no effect of allelic variation at the Glu-D1 locus was present. Epistatic effects between the Glu-I loci also contributed to the variation in loaf volume of the lines: i.e. the effect of allelic variation at Glu-A1 and Glu-B1 depended on the allele present at the Glu-D1. The contribution of the epistatic effects was about half the contribution of the additive effects, and should therefore be included in predictive models for bread-making quality.
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  • 86
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    Euphytica 56 (1991), S. 243-258 
    ISSN: 1573-5060
    Keywords: Triticum species ; wheat ; grain colour ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In addition to white and red grains, wheats with purple and blue grains may occur. Purple grain colour is caused by anthocyanins in the pericarp whereas blue colour is caused by anthocyanins in the aleurone layer. Purple grains occur in tetraploid wheats from Ethiopia, and in one bread wheat accession apparently native to China. Although the use of the purple and blue grain characters as markers has been suggested, their expression is often erratic, especially when heterozygous. No hexaploid wheat with blue grains was described prior to the artificial introgression of genes from diploid wheat and Agropyron species. The number of different sources of blue aleurone gene(s) from Agropyron elongatum is unknown. It is possible that with exchange between researchers the same or related accessions have been used at several research stations. Accessions of diploid wheats are known to possess blue aleurone. The breeding history of a number of purple and blue grained accessions is described. Research should indicate the source species of the gene for blue aleurone of the blue-grained Barevna.
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  • 87
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    Euphytica 57 (1991), S. 9-17 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; winter wheat ; intrinsic earliness ; photoperiod ; vernalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Development in wheat is strongly controlled by sensitivity to vernalization and photoperiod, and to a lesser degree by non-vernalizing temperature and intrinsic earliness. A method to measure effect of vernalization in wheats with winter habit is described. Twenty seven wheats with winter habit and eight with spring/facultative habit were studied, comprising breeding lines and cultivars with maturities suited to south-central New South Wales. Effect of vernalization on the development of these wheats was quantified by integrating responses to vernalizing treatments of differing duration. Intrinsic earliness was measured as time for vernalized seedlings to grow to ear emergece in an 18h photoperiod with day/night temperature of 21/16°C, and response to photoperiod as the difference in time to ear emergence between 9 and 18h daylengths. Integrated response to vernalization is sensitive to both cumulative and thresh-hold responses and is applicable to wheats of all habit type. Integrated response to vernalization and intrinsic earliness were positively associated within wheats with winter habit. Wheats were largely of restricted origin, so that there were few allelic differences at Vrn loci to disrupt this association, which suggests intrinsic earliness may modify response to vernalization. Though integrated response to vernalization was measured with artificial treatments, it was strongly associated with ear emergence for wheats with winter habit when grown at a site in New South Wales.
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  • 88
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita tritici ; leaf rust ; rust resistance ; slow rusting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genes conferring low seedling reaction to Mexican pathotypes of Puccinia recondita f. sp. tritici in 71 bread wheat (Triticum aestivum L.) cultivars from India and Pakistan were postulated. In total, 9 known and one unknown genes were identified, either singly or in combination: Lr1 (in 20 cultivars), Lr3 (5), Lr10 (21), Lr11 (1), Lr13 (43), Lr17 (5), Lr23 (14), Lr26 (2), Lr27 + Lr31 (2), and the unknown gene in 2 cultivars. Additional temperature-sensitive seedling resistance appeared to occur in 27 cultivars. This resistance in at least 15 cultivars appeared to be due to Lr34. Area under the disease progress curve (AUDPC) for these 27 cultivars indicated variable levels of adult plant resistance. Several other cultivars with high seedling infection types to one or more of the predominant field pathotypes were also partially resistant in the field. High levels of adult plant resistance occurred in some cultivars even in the absence of known seedling resistance genes with major effects.
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  • 89
    ISSN: 1573-5060
    Keywords: Adult plant resistance ; infection frequency ; latency period ; partial resistance ; Puccinia recondita f.sp. tritici ; race-specificity ; temperature sensitivity ; Triticum aestivum ; wheat ; wheat leaf rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Components of partial resistance, infection frequency and latency period, were determined in 71 winter and spring wheat genotypes in the seedling stage, after infection with three races of leaf rust (‘Felix 3B’, ‘Clement B’ and ‘Betuwe 85C’) at three different day/night temperature regimes (24/18°C, 18/12°C and 12/6°C). The genotypes were split into two groups and two separate experiments were carried out. Five genotypes, SVP 84039, Akabozu, Banco, BH 1146 and Orso, conferred a low infection frequency and a long latency period and Westphal 12A a long latency period, indicating a relatively high level of partial resistance. The correlation coefficient between infection frequency and latency period was low. Race-specificity was not found. There was a significant temperature effect on the latency period. In the second experiment the temperature x genotype interaction was significant. Temperature-response functions of transformed data demonstrated that the latency periods of four relatively resistant genotypes, Westphal 12A, Banco, BH 1146 and Orso and of Sarno and Mirela were most sensitive to temperature. The range between the genotypes with the longest and the shortest latency period was highest at 12°C. Therefore, low temperature regimes are preferred to distinguish differences in level of partial resistance.
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  • 90
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    Euphytica 58 (1991), S. 165-170 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; choice of parents ; distance analysis ; standardized potence ; character selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three groups of characters selected using three methods were used to calculate genetic distance among two groups of winter wheat lines using Euclidean distance based on principal components. These wheat lines were then clustered by the unweighted pair-group arithmetic average method. The relationship between genetic distance and standardized potence was seemingly influenced by genetic materials. In order to obtain promising crosses, it is advisable to select parents from intercluster rather than intracluster materials. The results suggested that the repeatability method was better than the conventional and weighting methods for selecting characters for distance analysis.
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  • 91
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    Plant growth regulation 10 (1991), S. 205-214 
    ISSN: 1573-5087
    Keywords: tillering ; wheat ; barley ; rice ; 2-phenoxypropionic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The properties of various mono- and di-substituted analogues of 2-phenoxypropionic acid as inhibitors of tillering were investigated on wheat, barley and rice. Highest levels of activity were shown by (R)2-Cl,5-Cl, (R)2-Cl,5-F, and (R)2-Cl,5-methyl analogues. Few or no signs of phytotoxic effects (leaf chlorosis or necrosis) were evident on wheat or barley following spray application of these compounds. Rice was both more susceptible to inhibition of tillering and phytotoxic effects. However, almost complete inhibition of tillering was achieved by application of some compounds to rice with little or no phytotoxicity. Comparisons were made between the properties of these compounds and commercially used phenoxyacetic and phenoxypropionic herbicides and plant growth regulators. Dichlorprop inhibited tillering in rice, fenoprop in wheat and rice, and fluroxypyr in wheat, all without phytotoxic effects.
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  • 92
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    Plant cell, tissue and organ culture 26 (1991), S. 141-146 
    ISSN: 1573-5044
    Keywords: calluses mutagenesis ; embryo mutagenesis ; EMS somatic effect ; immature ; embryo mutagenesis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutation experiments require careful selection of a mutagen with characteristics suited to the tissue source and mutagenesis objective, and an appropriate treatment regime. The objectives of the present investigation were:-to compare the ability of immature embryos to initiate calluses and calluses derived from immature embryos to survive and grow after being treated with ethyl methanesulfonate (EMS) doses of 0.2, 0.4, 0.8, 1.2, and 1.6% (v/v) and-to compare these results with those published for seed mutagenesis. To determine if the response of tissue source to EMS treatment varied with genotype, tissues from two spring wheat cultivars, ‘Angus’ and ‘Pavon 76’, were used. The combined analysis of variance detected highly significant differences (p≤0.01) among doses. The higher the dose, the lower the tissue survival in each tissue source. No significant differences were detected between cultivars, tissue sources, in the cultivar by dose interaction, tissue source by dose interaction, tissue source by cultivar interaction, or tissue source by cultivar by dose interaction. Hence, both cultivars and tissue sources responded similarly to EMS doses. The predicted LD20 are 0.35%±0.08% for the immature embryo treatment, and 0.36%±0.10% for the callus treatment. The predicted LD50 are 0.82%±0.13% for the immature embryo treatment, and 0.77%±0.13% for the callus treatment. These results were very similar to published results for seed mutagenesis, hence seed mutagenesis research may be applicable to immature embryo and callus mutagenesis.
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  • 93
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    Plant cell, tissue and organ culture 24 (1991), S. 233-236 
    ISSN: 1573-5044
    Keywords: anther culture ; cultivars ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-two cultivars and lines of winter and spring wheat (Triticum aestivum L.) were studied, most for the first time, for their anther culture response. The response was genotype dependent. Plants grown in the field gave higher callus induction frequency than those grown in the greenhouse and the controlled environment chamber. Donor plants grown in a season of low drought stress as compared to a season of severe drought stress resulted in a higher frequency of callus induction. Spherical microcalli were observed in two wheat genotypes in some of only those anthers that were placed with only one loculus in contact with the medium. Wheat lines that were more responsive to anther culture were identified.
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  • 94
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    Plant cell, tissue and organ culture 25 (1991), S. 199-208 
    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; cocultivation ; enzymatic digestion ; tissue culture ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agrobacterium attached to wheat embryos in vitro. This attachment was plasmid independent, and occurred on both wounded and unwounded cell surfaces. The pattern of attachment clearly demonstrated that bacterial attachment to cereal cells follows the same trends observed for dicotyledonous plants. During the inoculation period the bacterial cells attach to the plant cell walls either with lateral or polar orientation. Wounding (mechanical or enzymatic) preferentially promoted adherence of the bacteria at the wound site, however, attachment was not wound dependent.
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  • 95
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    Plant cell, tissue and organ culture 25 (1991), S. 209-218 
    ISSN: 1573-5044
    Keywords: Agrobacterium tumefaciens ; cocultivation ; tissue culture ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA can be transferred by Agrobacterium tumefaciens to wheat, albeit at very low frequencies. Transfer of agrobacterial DNA occurred in cultures where the embryos had been subjected to partial enzymatic digestion prior to cocultivation with the bacteria. It is unclear whether this is by the normal process mediated by the Ti virulence genes and the border repeats of the T-DNA. The Southern hybridization patterns indicate that in one cell line the T-DNA had undergone extensive rearrangements, and might indicate that the process of T-DNA transfer and integration might differ in the case of cereals. This could suggest the method of transfer and ultimately the expression of these genes in cereal cells may be different to that observed in other monocotyledonous and dicotyledonous species.
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  • 96
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    Nutrient cycling in agroecosystems 28 (1991), S. 67-72 
    ISSN: 1573-0867
    Keywords: Phosphate adsorption ; external P requirement ; wheat ; fertilizer requirements ; calcareous soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Five field experiments involving P application rates from 0 to 66 kg P ha−1 were conducted on irrigated wheat at Tandojam, Pakistan. The soils belonged to two great soil groups, Torrifluvent and Camborthid. All soils were calcareous. Olsen-P contents ranged from 3.5 to 6.3 mg P kg−1. Phosphate sorption curves were developed for soils from control (no P) plots at each site. Concentrations of P in solution established by fertilization in the field as estimated from the sorption curves ranged from 0.008 to 0.16mg P L−1. Actual grain yields were converted to relative grain yields and plotted against corresponding concentrations of P in solution. Yield response to P application was obtained in each experiment. Control plot yields ranged from 57 to 89% of maximum yield of respective experiments. Phosphorus requirements of wheat were 0.032 mg L−1 for 95% yield as determined from a composite yield response curve. Predicted quantities of P required to attain 0.032 mg P L−1 ranged from 18 to 29 kg P ha−1. The results of the study suggest that the P sorption approach can be used as a rational basis for making P fertilizer recommendations for various soil-crop combinations.
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  • 97
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    Nutrient cycling in agroecosystems 30 (1991), S. 1-7 
    ISSN: 1573-0867
    Keywords: Chloride ; pH ; residual effect ; sandy soil ; selenate ; selenite ; sulphate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In areas where soils supply inadequate Se to crops to meet animal nutritional needs, it may be necessary to supplement Se in some fashion. This greenhouse experiment was designed to study the effect of selenate and selenite, incorporated with a Cl- or a SO4-based NPK (16-5-12) fertilizer added to a low-Se soil, on the Se concentration in wheat (Triticum aestivum L.). The sandy soil was limed to pH 5.5 and 6.5 prior to receiving 0, 0.03, or 0.06 mg Se kg−1 of soil with each NPK source each year for two years. The SO4-based NPK fertilizer treatment was used only at the 0.06 mg Se kg−1 rate. In the third year, the residual effect of the Se applied in the first 2 years was studied. The grain yield among treatments differed significantly but the effects of the rate or source were not consistent in all the years. However, significantly higher yields were obtained at pH 6.5 than at pH 5.5. Selenium concentration and uptake in wheat grain increased in proportion to the applied selenate but not selenite. However, selenite increased the Se concentration and uptake over the control. The concentration and uptake of Se were higher from selenate than from selenite. The effect of the Cl- and SO4-based NPK fertilizers was different on selenate and selenite. Selenium accumulation in wheat grain was greater from the Cl-based NPK fertilizer when selenate was used but it was greater from the SO4-based NPK fertilizer when selenite was used. The Se concentration in wheat, especially from selenate, was generally higher at the higher pH level except in the first year where it was higher at the lower pH level. The uptake of Se from the Se-enriched NPK fertilizers was generally as high as from the Se applied separately. The residual effect of both sources of Se applied in the first 2 years was very poor in the third year. The results suggest that the Se concentration in wheat grain can be increased by incorporating the Se salts in the NPK fertilizers but more selenate is recovered than selenite. Selenate should, however, be incorporated in a Cl-based NPK fertilizer to avoid the ionic competition from SO4 ions in the SO4-based NPK fertilizer. The uptake of Se is generally higher at pH 6.5 than at pH 5.5.
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    Nutrient cycling in agroecosystems 30 (1991), S. 39-46 
    ISSN: 1573-0867
    Keywords: Rock phosphate ; single superphosphate ; P uptake ; VAM fungi ; maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of inoculation with fourteen endomycorrhizal species belonging to the generaGigaspora, Scutellospora, Glomus, Acaulospora andEntrophospora on the growth of maize (Zea mays L.) was evaluated under glasshouse conditions in an unsterilized tropical virgin soil using two P sources with different solubility. In both P treatments the population of indigenous mycorrhiza species was not affected and growth of maize was enhanced. Introducing VAM species additionally modified the growth pattern of maize. Using a low-grade rock phosphate (Patos de Minas) from Brazil all endophytes with exception ofGigaspora margarita, Scutellospora verrucosa, Scutellospora gregaria, Entrophospora colombiana andGlomus pallidum improved shoot dry weight. In the treatment with single superphosphate, dry matter production was not significantly enhanced byGigaspora margarita, Gigaspora gigantea, Scutellospora verrucosa, Scutellospora reticulata, Scutellospora gilmorei andGlomus manihotis. When rock phosphate was added root fresh weights were enhanced only by three endophytes (Gigaspora margarita, Gigaspora gigantea andAcaulospora rehmii); with single superphosphate none had a significant effect. The percentage of P in shoots was almost equal in non-inoculated and inoculated plants and yield responses did not always follow the pattern of P uptake. Mycorrhizal root infection was always highest in the treatment with single superphosphate and in most cases a correlation with plant growth was found. The present results show that introduced vesicular arbuscular mycorrhiza species differently promote growth of maize according to their adaptability to the P source and to their capability to compete with native VAM endophytes.
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  • 99
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    Springer
    Agroforestry systems 14 (1991), S. 193-205 
    ISSN: 1572-9680
    Keywords: Acacia albida ; intercropping ; maize ; green gram ; coastal lowlands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Long-term agroforestry demonstrations/trials using Acacia albida and other nitrogen fixing multipurpose trees/shrubs were initiated in mid-1982 to assess soil and crop productivity at a coastal lowland site characterized by low soil fertility, weed problems and consequent poor crop yields. Growth performance (height and diameter at breast height, dbh) of Acacia albida under eight densities rotationally intercropped with maize (Zea mays) and green gram (Phaseolus aureus), crop grain yields, soil fertility changes and weed control were assessed for a 5-year period (May 1982 to March 1987). A parallel-row systematic spacing field layout was used. Intercropped Acacia albida mean hight and dbh were 140 and 24% respectively higher than tree-only controls by the fifth year. Growth rate was low during the first year but increased in subsequent years to mean height and dbh of 9 m and 10 cm respectively by March 1987. While differences in dbh were significant, those between stand heights were not. Crop yields, especially under higher tree densities, declined considerably due to unexpected shade which also caused significant reductions in weed biomass. Soil fertility levels remained unchanged during the experimental period relative to the initial status, and differences between the intercropped Acacia albida plots and the tree — or crop — only control appeared not to be significant. We conclude that an understanding of the mechanism regulating leaf fall/retention phenomena of Acacia albida is crucial towards determining the intercropping potentials of the species.
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  • 100
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    Springer
    Human ecology 19 (1991), S. 389-418 
    ISSN: 1572-9915
    Keywords: maize ; indigenous knowledge ; technological change ; Mexico
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract This paper presents a case study of the relationship between farmers' knowledge of maize varieties and their selection and management of these varieties under conditions of technological change. Research for this paper was done among Spanish-speaking small farmers in an ejidoof central Chiapas, Mexico. This ejido is well integrated into the market, and the use of modem technologies is widespread. This research demonstrates that farmers have an extensive and widely shared knowledge of their maize varieties. This knowledge reflects objective maize characteristics. Variation occurs in the farmers' selection and management of maize varieties, but on average the variation deviates from a random pattern in the direction predicted by the farmers' knowledge base. They have incorporated the technological changes brought about by development into their knowledge base. Farmers maintain maize varieties with contrasting traits, and their knowledge base provides important information about which traits and constraints are important to them.
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