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  • Articles  (2,596)
  • Springer  (2,437)
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  • 1988  (2,596)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (2,596)
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  • Articles  (2,596)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European journal of wildlife research 34 (1988), S. 232-241 
    ISSN: 1439-0574
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary To evaluate the feeding pressure exerted by roe deer(Capreolus capreolus), on vegetation, the biomass within reach of feeding animals was measured in the Chizé forest (Deux Sèvres, France) in 2 types of woodland (oak and beech), and for 2 levels of population density: a high density of 20 animals/100 hectares (inside a fenced reserve) and a low density of 5 animals/100 hectares (outside the reserve). The biomass of vegetation was sampled 3 times (spring, end of summer, winter) using the same procedure each time. In the oakwood, total biomass was greater in areas of low density population than in areas with high density. In the beechwood, there was no significant difference in total biomass according to population density. In all cases, the maximum total biomass was observed in the spring sampling. Some differences were noted in the composition of the vegetation between the area inside the game reserve and the area outside the reserve.Hedera helix was very abundant outside the reserve andRuscus aculeatus and mosses were more frequent in the reserve. The feeding impact of roe deer on vegetation determines a change in the floral equilibrium permitting an increase in the abundance of plants not eaten by roe deer and causing a decrease of plants favoured by the deer.
    Abstract: Résumé Dans le but d'évaluer l'impact du chevreuil sur la végétation, la biomasse accessible à ces animaux a été évaluée dans 2 types d'association végétale (une chênaie et une hêtraie) pour 2 niveaux de densité de population dans la forêt domaniale de Chizé (Deux Sèvres, France): forte densité (20 animaux pour 100 hectares) dans la Réserve Nationale de Chasse et faible densité (5 animaux pour 100 hectares) à l'extérieur de la Réserve. Trois séries de relevés ont été effectuées avec la même méthode (printemps, fin de l'été, hiver). Dans la chênaie, la biomasse totale est plus importante en faible densité qu'en forte densité. Ce phénomène n'est pas observé dans la hêtraie. Dans tous les cas, elle est maximale au printemps. Des differences de la composition spécifique de la végétation sont observées entre la zone en réserve et l'extérieur. Le lierre(Hedera helix) est très abondant hors de la réserve. Le fragon(Ruscus aculeatus) et les mousses sont plus développées dans la réserve. L'impact du chevreuil se traduirait par une modification de la composition de l'équilibre végétal, le développement des espèces refusées par cet animal et la raréfaction des espèces appétentes.
    Notes: Zusammenfassung Zur Beurteilung des Einflusses des Rehwildes (Capreolus capreolus) auf die Vegetation wurde die diesen Tieren zugängliche Biomasse in zwei Waldgesellschaften (Eichen- und Buchenwald) mit unterschiedlicher Populationsdichte (hohe Dichte: 20 Stück/100 ha; niedrige Dichte 5 Stück/100 ha) im Staatsforst von Chizé (Frankreich) erhoben. Drei Probeaufnahmen wurden mittels der gleichen Methode (Frühjahr, Spätsommer, Winter) durchgeführt. Die Gesamtbiomasse liegt im Eichenwald höher bei niedriger als bei starker Populationsdichte. Gleiches Phänomen kann im Buchenwald nicht festgestellt werden. In allen Fällen wird die maximale Biomasse im Frühjahr erreicht. Unterschiede in der spezifischen Vegetationszusammensetzung zwischen Gehege-und Freiflächen konnten beobachtet werden. Efeu (Hedera helix) ist außerhalb des Geheges reichlich vorhanden. Segge (Ruscus aculeatus) und Moosarten sind im Gehege stark vertreten. Der Einfluß der Rehe wird durch die Veränderung des Vegetationsgleichgewichtes, der Entwicklung von verweigerten und Seltenwerden der bevorzugten Pflanzenarten, eindeutig.
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  • 2
    Electronic Resource
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    Springer
    European journal of wildlife research 34 (1988), S. 262-276 
    ISSN: 1439-0574
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 3
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    Springer
    European journal of nutrition 27 (1988), S. 71-74 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
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  • 4
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    Springer
    European journal of nutrition 27 (1988), S. 57-70 
    ISSN: 1436-6215
    Keywords: Retinol ; Vitamin-A-Status ; Vitamin-A-Serumbestimmung ; Hypervitaminose A ; Hypovitaminose A ; retinol ; vitamin A status ; vitamin A serum determination ; hypervitaminosis A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary As demonstrated in the literature on vitamin A metabolism and homeostasis of retinol in serum, the concentration of retinol in serum is regulated very exactly if the liver stores are within the physiological range (20–300 Μg/g liver). Therefore, the serum level indicates the status of vitamin A storage only if there is an extreme depletion or overconsumption of vitamin A. At marginal depletion, however, there is damage to peripheral tissue before changes in the vitamin A level in serum occur. At the beginning of hypervitaminosis A, changes in the level of vitamin A in serum also occur later. Therefore, the determination of vitamin A in serum gives no information on the adequacy of liver reserves for judging the necessity of a substitution.
    Notes: Zusammenfassung Wie viele Arbeiten über den Vitamin-A-Metabolismus und Retinol-Serumhomöostase zeigten, unterliegt die Retinol-Serum-Konzentration im physiologischen Bereich der Leberreserven (20–300 Μg/g Leber) einer strengen Regulation. Daher gibt der Serumspiegel nur bei einem extremen Mangel oder bei übersättigung mit Vitamin A Auskunft über die Speichervorkommen. Im Grenzbereich, dem marginalen Mangel, kommt es aber schon zur Schädigung peripherer Gewebe, bevor sich der Retinol-Serumspiegel verändert. Auch bei beginnender Hypervitaminose A zeigen sich Veränderungen im Serumspiegel erst zu einem späteren Zeitpunkt. Daher ist die Bestimmung des Vitamin-A-Serumspiegels kein guter Indikator für den Vitamin-A-Status und die Beurteilung der Notwendigkeit einer Substitution.
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  • 5
    ISSN: 1436-6215
    Keywords: mental retardation ; obesity ; body size ; serumlipids ; low-energydiet ; geistige Behinderung ; Übergewicht ; Serumlipide ; kalorienarmeDiät
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Geistig behinderte, übergewichtige Patienten, die in einem geschlossenen Kollektiv lebten, verwendeten neun Monate lang eine kalorienreduzierte Kost (4,2–4,6 MJ). Während der Untersuchungsperiode war eine Körpergewichtsverminderung von 13±5,5 kg bei den Männern und 16±2,6 kg bei den Frauen zu beobachten. Vor der Diättherapie und im neunten Monat wurden anthropometrische Untersuchungen durchgeführt. Wir konnten feststellen, daß der prozentuale Anteil des Körperfetts, nach BMI berechnet, deutlich abnahm. Anhand der Hautfaltmessungen dagegen zeigte sich diese Verminderung weniger deutlich. Das ist Wahrscheinlich auch auf die Schwierigkeiten zurückzuführen, die während der Messungen infolge körperlicher Mißbildungen auftraten. Die klinischen Laboruntersuchungen, die vor Beginn der diätetischen Behandlung sowie im vierten und siebten Monat durchgeführt wurden, weisen darauf hin, daß eine kalorienarme Kost mit ausgeglichenem Nährstoff- bzw. Eiweißgehalt dem Eiweißstoffwechsel nicht schadet und den Triglycerid- und Cholesterinspiegel im Serum günstig beeinflußt, andererseits aber eine ungewünschte Cholesterinverminderung in der HDL-Fraktion verursacht.
    Notes: Summary Mentally retarded obese in-patients were fed by low-energy diet (4.2–4.6 MJ) for 9 months. During this period, an average of 13±4.5 kg loss of body mass occurred in men and 16±2.7 kg in women. Anthropometric measurements were performed before starting the dietotherapy and in the ninth month. Changes of body fat could be followed well when calculated according to BMI. Less reliable results were obtained with skinfold thickness Measurements, presumably due to body deformities. Results of clinical laboratory tests, which were carried out before starting the dietotherapy and in the fourth and seventh months, suggested that a low-energy-containing diet with balanced nutrient content and adequate protein intake did not lrnpair protein metabolism, favourably affected serum triglyceride and cholesterol levels, but resulted in an unfavourable decrease in the HDL-cholesterol content.
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  • 6
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    Springer
    European journal of nutrition 27 (1988), S. 126-136 
    ISSN: 1436-6215
    Keywords: marginalerZinkmangel ; humoraleImmunität ; Immunglobuline der Ratte ; marginalzinc deficiency ; humoralimmunity ; ratimmunoglobulins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The purpose of this experiment was to examine the effects of a marginal zinc deficiency on serum immunoglobulin (Ig) IgM and IgG1 levels. 60 weanling female Sprague-Dawley rats were divided into six groups of ten animals each. Two control groups (73.7 ppm Zn) and two zinc-deficient groups (4.3 ppm Zn) were fed a semisynthetic casein diet ad libitum. The animals of two further groups received the control diet (73.7 ppm Zn) pair-fed to the zinc-deficient animals. After 15 days the rats of one group of each treatment were infected i.m. with a single injection (0.2 ml) of a bovine enterovirus, apathogenous to rats. 3 weeks later, all rats were anesthetized with ether and decapitated. Infection of the rats with the bovine enterovirus had no effect on the serum immunoglobulin level. The marginal zinc deficiency did not alter the IgM and IgG1 serum concentrations at all either. It remains to be examined whether other Ig-classes will react to marginal zinc deficiency. Also, the effect of severe zinc deficiency on the humoral immune system must be studied.
    Notes: Zusammenfassung In einem Versuch mit 60 weiblichen entwöhnten Sprague-Dawley-Ratten wurde geprüft, ob marginaler Zinkmangel direkte Auswirkungen auf die Serumimmunglobulinspiegel IgM und IgG1 zeigt. Die Ratten wurden in 6 Behandlungsgruppen zu je 10 Tieren eingeteilt. Zwei Kontrollgruppen (73,7 ppm Zn) und die beiden marginalen Zinkmangelgruppen (4,3 ppm Zn) wurden ad libitum mit einer halbsynthetischen Diät auf Caseinbasis versorgt. Die Tiere zweier weiterer Gruppen erhielten pair-fed zu den Mangeltieren eine Kontrolldiät mit ebenfalls 73,7 ppm Zn. 15 Tage nach Verfüttern dieser Diäten wurde jeweils eine Gruppe der unterschiedlich versorgten Tiere mit einem für Ratten apathogenen Rinderenterovirus (0,2 ml pro Ratte) i.m. infiziert. 3 Wochen später wurden alle Tiere mit Ether betäubt und dekapitiert. Die Infektion der Ratten mit dem bovinen Rinderenterovirus blieb ohne Einfluß auf den Immunglobulin-(Ig-)Spiegel. Auch der marginale Zinkmangelzustand, wie er in der vorliegenden Untersuchung auftrat, führte bei den Versuchstieren zu keinen Veränderungen in der IgM- und IgG1-Konzentration des Serums. Es bleibt zu untersuchen, ob andere Immunglobulinklassen eher auf marginalen Zinkmangel ansprechen oder welchen Einfluß ein extremer Zinkmangel bei der Ratte ausübt.
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  • 7
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    Springer
    European journal of nutrition 27 (1988), S. 201-205 
    ISSN: 1436-6215
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
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  • 8
    ISSN: 1436-6215
    Keywords: cholesterol ; triglycerides ; lipoproteins ; ω 3 fatty acids ; Cholesterol ; Triglyceride ; Lipoproteine ; ω-3-Fettsäuren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung In einem Kurzzeit-Experiment wurde an gesunden, normolipidämischen Probanden geprüft, ob durch einen mäßig hohen Fischverzehr der Gehalt an Plasma- und Lipoproteinlipiden gesenkt werden kann. 8 Teilnehmer aßen eine Woche lang täglich 100 g Makrele (entsprechend etwa 2,5–3 g ω-3-Fettsäuren). Die Triglycerid-Konzentrationen im Plasma und in den VLDL und LDL waren nach der Fischdiät um 40, 46,7 und 38,5 % niedriger als zu Beginn des Experiments. Die Plasmacholesterol-Konzentration war nur geringfügig erniedrigt, das Verhältnis HDL/Gesamt-Cholesterol aber signifikant erhöht. Die Studie zeigt, daß eine moderate Steigerung des Fischverzehrs bei gesunden, normolipidämischen Probanden innerhalb einer Woche — selbst auf der Basis einer nicht strikt kontrollierten Diät — die Lipidspiegel verändern kann.
    Notes: Summary The effect of mackerel consumption on plasma and lipoprotein lipid concentrations was studied in a seven-day experiment in eight healthy, normolipidemic subjects. Participants ate about 100 g mackerel (corresponding to about 2.5–3 g ω3 fatty acids daily. The mean triglyceride concentrations in total plasma, VLDL, and LDL were significantly reduced by 40, 46.7, and 38.5 % respectively after fish consumption. There was also a small (non-significant) reduction of plasma cholesterol and a (significant) increase of the ratio of HDL/total plasma cholesterol. These data show that a moderately increased intake of ω-3 fatty acids by fish food can change lipid characteristics in healthy normolipidemic individuals within a short-time period, even on a free diet.
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  • 9
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    Springer
    European journal of nutrition 27 (1988), S. 236-243 
    ISSN: 1436-6215
    Keywords: cholesterol feeding ; spermatogenesis ; Leydig's cell ; VLDL/LDL-cholesterol ; Cholesterinfütterung ; Spermatogenese ; Leydig-Zellen ; VLDL/HDL-Cholesterin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Cholesterinfütterung über 120 Tage beeinträchtigt bei männlichen Ratten und Kaninchen die Testikularfunktion. Die Produktion von sekundären Spermatozyten wird bei Ratten und Kaninchen um 26,1 % bzw. 46,9 % reduziert. Ferner kommt es zu einer signifikanten Reduktion der Spermatid-Population, der Samenkanälchen und der Dimension der Zellkerne der Leydig-Zellen. Im Aortenendothel finden sich bei der Ratte einige Alterationen, beim Kaninchen dagegen voll ausgeprägte atherosklerotische Plaques. Gesamtcholesterin, Triglyceride und Phospholipide in den Testes nehmen zu, während der Gehalt an Glykogen in den Testes abnimmt. Die Cholesterinkonzentrationen im Serum sind bei Ratten dreifach, bei Kaninchen siebenfach erhöht, wobei LDL-Cholesterin ansteigt, der Quotient HDL-Cholesterin/Gesamtcholesterin dagegen signifikant abfällt. Als Schlußfolgerung ergibt sich, daß Hypercholesterinämie neben dem Aortenepithelium auch die Hodenfunktion beeinträchtigt.
    Notes: Summary Cholesterol fed to male rats and rabbits for 120 days impaired testicular function. The production of secondary spermatocytes in rats and rabbits was reduced by 26.1% and 46.9%, respectively. There was significant reduction in spermatid cell population, seminiferous tubules, and Leydig's cell nuclear dimensions. In rats aortic endothelium showed some alterations while in rabbits full-blown atherosclerotic plaque containing a necrotic core and a proliferative fibrous cap was found. Total cholesterol, triglycerides and phospholipids of the testes were increased whereas, testicular glycogen was significantly reduced. Three- and sevenfold increases were noticed in the serum cholesterol levels of rats and rabbits. The LDL cholesterol values were increased while the HDL cholesterol/Total cholesterol ratio was significantly decreased (P〈0.001). It is concluded that hypercholesteromia affects the testicular function and aortic endothelium.
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  • 10
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    European journal of nutrition 27 (1988), S. 244-251 
    ISSN: 1436-6215
    Keywords: Phytinsäure/Zink-Quotient ; Vollkornbrot ; Knäckebrot ; Zink alkalische ; Phosphatase ; phytic acid/zinc ratio ; wholemeal bread ; crispbread ; zinc alkaline ; phosphatase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary A study was untertaken to determine whether the reduction of the molar phytic acid/zinc ratio by enrichment of wholemeal and crispbread with zinc improves zinc availability to the same extent as does the reduction of phytic acid in whole meal cereal products. For three weeks, five diets (three samples of wholemeal bread and two samples of crispbread)) were fed to growing rats. Significant increases in bone-zinc deposition and activity of serum alkaline phosphatase were induced by those bread samples in which the phytic acid/zinc ratio had been lowered either by enrichment with zinc or by reduction of phytic acid (enzymatic hydrolysis by adjusting the pH-value of the dough with lactic acid) when compared with untreated bread samples. Weight gain of the animals differed little between the groups. The results suggest that it is possible to improve zinc availability in wholemeal cereal products high in phytate by enrichment with zinc.
    Notes: Zusammenfassung Es wurde geprüft, ob die Verringerung des molaren Phytinsäure/Zink-Quotienten in Vollkorn- oder Knäckebrot mit einem hohen Phytinsäuregehalt durch Anreicherung mit Zink die gleiche günstige Wirkung auf die Zinkverfügbarkeit hat wie die Reduzierung der Phytinsäure in Vollkornprodukten. In einem dreiwöchigen Versuch wurden 5 Diäten (3 Schrotbrot- und 2 Knäckebrotproben) an wachsende Ratten verfüttert. Die Proben, in denen der Phytinsäure/Zink-Quotient durch Anreicherung mit Zink oder durch Reduzierung der Phytinsäure (enzymatische Hydrolyse durch Einstellung des pH-Wertes des Teiges mit Milchsäure) verringert war, bewirkten eine signifikante Erhöhung der Zinkeinlagerung im Femur und der Aktivität der alkalischen Phosphatase im Serum der Tiere im Vergleich zu unbehandelten Brotproben. Das Wachstum der Tiere zeigte nur geringe Unterschiede zwischen den 5 Gruppen. Die Ergebnisse lassen erkennen, daß eine Verbesserung der Zinkverfügbarkeit in Getreidevollkornprodukten mit hohem Phytatgehalt durch Anreicherung mit Zink erzielt werden kann.
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  • 11
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    European journal of nutrition 27 (1988), S. 252-265 
    ISSN: 1436-6215
    Keywords: cow'smilk ; humanmilk ; high performance liquid chromatography (HPLC) ; infant formulas ; phospholipids ; Frauenmilch ; Hochdruck-Flüssigkeitschromatographie (HPLC) ; Kuhmilch ; Phospholipide ; Säuglingsnahrungen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Mit Hilfe einer speziell hierfür entwickelten (6) Hochdruckflüssigkeitschromatographie-Methode (HPLC) wurde der Phospholipidgehalt (PL) von Frauenmilch, Kuhmilch und verschiedenen Säuglingsnahrungen bestimmt. Die Untersuchungen zeigen, daß der Gehalt an Phosphatidylinositol (PI), Phosphatidyläthanolamin (PE), Phosphatidylcholin (PC) und Sphingomyelin (SP) durch Homogenisieren und Pasteurisieren von Kuhmilch nicht verändert wird. Der Gehalt an Phosphatidylglycerol (PG) liegt hier, wie bei der Frauenmilch, unter der Nachweisgrenze. Auch bei den anderen untersuchten PL konnten zwischen Frauen- und Kuhmilch keine nennenswerten Unterschiede festgestellt werden. Der Gehalt einiger PL verändert sich im Verlauf der Spätschwangerschaft und der Post-partum-Periode charakteristisch. So liegt der Gehalt an PI, PC und SP in der Vormilch deutlich über dem Gehalt reifer Frauenmilch. Bereits vor Beginn der Wehen zeigen alle PL einen mehr oder weniger scharfen Abfall. So verliert Vormilch vor der Geburt 70 % des Gehaltes, den PL-Proben sechs Wochen vor der Geburt zeigen. Die PG-Gehalte sind allgemein in der gesamten Beobachtungsperiode sehr niedrig. Im Gegensatz zu den anderen Parametern erholt sich der PC-Gehalt drei Wochen nach der Geburt wieder, was als Ausdruck eines endogenen Surfactant-Regelmechanismus betrachtet werden kann. Weiterhin wurden 13 verschiedene Säuglingsnahrungen im Vergleich mit Kuhmilch und Frauenmilch untersucht. Dabei wurden teilweise erhebliche Unterschiede zwischen adaptierter Milch, teilweise adaptierter Milch und Spezialnahrungen, aber auch innerhalb dieser Gruppen, gefunden. Keines der bestimmten PL konnte in allen Säuglingsnahrungen nachgewiesen werden. Mit Ausnahme einiger Milupa-Säuglingsnahrungen war der PG-Gehalt allgemein niedrig. PE und PI wurden nur in einigen Spezialnahrungen nicht gefunden. Die meisten Säuglingsnahrungen enthalten hohe Gehalte an SP, die teilweise höher als der Gehalt an PC liegen. Allgemein kann gesagt werden, daß die untersuchten Säuglingsnahrungen z.T. wesentlich andere Phospholipidmuster als Frauen- und Kuhmilch aufweisen und daß zwischen einzelnen Säuglingsnahrungen erhebliche Unterschiede in der Zusammensetzung bestehen. In den meisten der untersuchten Nahrungen ist der Phospholipidgehalt so hoch, daß er durchaus als Phospholipidquelle für das Neugeborene genutzt werden kann.
    Notes: Summary The phospholipid (PL) content of human milk, cow's milk, and various infant formulas was determined by recently developed high performance liquid chromatography (6). As the examinations promised, the content of phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylcholin (PC), and sphingomyelin (SP) was not changed by homogenization and pasteurization of cow's milk. Levels of phosphatidylglycerol (PG) were below the detection limit. Furthermore it has been proved that human milk and cow's milk are more or less identical in PL content. Some of the PL in human milk varies during the course of pregnancy and postpartum. PI, PC, and SP content in the prepartum mammarial secretion lies above the average content of mature human milk after delivery. Before the contractions start, all the PL examined show a more or less considerable decrease. PC drops to 30 % of the value at the beginning of the examination six weeks before delivery. PG contents are very low throughout the whole period. Contrary to the others, PC content recovers three weeks after delivery, which may be the result of the endogenous surfactant replacement system. To compare PL content with human milk and cow's milk, 13 different infant formulas have been examined. There are considerable differences to be found in and among adapted milk, partially adapted milk, and special formulas. None of the PL examined could be found in all the infant formulas, where PG content was usually low, except in some Milupa formulas. PE and PI were not to be found in some special formulas. Most of the formulas contain high amounts of SP, in some cases higher than the amount of PC. To a certain extent infant formulas contain a considerably greater amount of other PL concentrations than human milk and cow's milk. In most of the formulas examined the PL content is generally so high, that it can be used as a source of PL for the newborn.
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  • 12
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    European journal of nutrition 27 (1988), S. 119-125 
    ISSN: 1436-6215
    Keywords: vitamin A ; development offetal heart ; DNA ; RNA ; protein ; rat ; Vitamin A ; Entwicklungfetaler Organe ; DNA ; RNA ; Protein ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung Die Auswirkung einer beschränkten Vitamin-A-Zufuhr bei trächtigen Ratten auf die biochemische Entwicklung des fetalen Herzens wurde untersucht. Der Vitamin-A-Status der weiblichen Ratten wurde durch Angebot von geringen, mittleren und hohen Mengen an Vitamin A während Trächtigkeit und Säugeperiode verändert (6,40 und 100 μg Retinol/Tag/kg Körpergewicht). Der Vitamin-A-Status der Muttertiere verursachte eine Veränderung in der Entwicklung des fetalen Herzens in bezug auf Gewebegewicht, DNA-, RNA- und Proteingehalt sowie der Biosynthese von DNA und Proteinen aus jeweils [3H]-Thymidin und [3H]-Leucin. In den sich entwickelnden Rattenherzen wurde eine dosisabhängige Wirkung von Vitamin A auf den DNA-, RNA- sowie den Proteinstoffwechsel beobachtet. Eine angemessene Versorgung der Muttertiere mit Vitamin A ist entscheidend für die normale Entwicklung fetaler Organe.
    Notes: Summary The effect of restricted vitamin A supply to the mother on the biochemical development of the heart in rats has been studied. The vitamin A status of female rats was varied by supplying low, medium and adequate amounts (6, 40, 100 μg retinol/day/kg body weight respectively) of vitamin A during pregnancy and suckling. The vitamin A status of the mother caused an alteration in the developmental pattern of fetal heart in parameters of tissue weight, DNA, RNA and protein contents and the biosynthesis of DNA and protein from [3H]-thymidine and [3H]-leucine respectively. A dose-dependent effect of vitamin A on the metabolism of DNA, RNA and protein was observed in the developing rat hearts. Hence, emphasis should be given to the adequate availability of vitamin A to dams for the normal growth and development of fetal organs.
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  • 13
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    European journal of nutrition 27 (1988), S. 155-169 
    ISSN: 1436-6215
    Keywords: acesulfame ; anion transport ; cyclamate ; saccharin ; streptococcus mutans ; sweetener ; Acesulfam ; Anionentransport ; Cyclamat ; Saccharin ; Streptococcus mutans ; Süßstoff
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Zusammenfassung In einem 1-Octanol/Phosphatpuffersystem war Saccharin viel stärker lipophil, als aus seiner Dissoziationskonstante abgeleitet werden kann; diese dagegen bestimmte die Verteilung von Acesulfam und Cyclamat. Die Aufnahme von Saccharin in S. mutans ergab eine 30-bis 40fache Anreicherung dieses Süßstoffs gegenüber dem Medium. Acesulfam und Cyclamat verteilten sich zwischen Zellen und Medium im wesentlichen nach einem diffusionskontrollierten Vorgang. Die Aufnahme von Saccharin in S. mutans erwies sich als von einer gleichzeitigen Zuckerfermentation abhängig, ferner auch vom Außen-pH, von der Süßstoff-Konzentration und der Zellzahl. Ohne Glykolyse — z. B. aufgrund des Verbrauchs der vorgelegten Saccharose, wegen eines zu sauren pH-Wertes im Medium oder wegen Anwesenheit von Glykolyse-Inhibitoren — war die Aufnahme von Saccharin ebenso nur diffusionskontrolliert wie die des Acesulfams und die des Cyclamats. Durch Zugabe von L-Lactat ins Medium, wodurch die Richtung des Lactat-Gradienten umgekehrt wurde, war die Saccharin-Aufnahme gehemmt. Die Aktivierungsenergie der Saccharin-Aufnahme betrug rund 18 kJ/Mol, während die Glykolyse selbst je nach Versuchsbedingungen 82–98 kJ/Mol erforderte. Bis zu 100 attomol Saccharin wurden pro Bakterienzelle gefunden. Daraus läßt sich schließen, daß die Cytomembran von S. mutans an der Vermittlung der Hemmeffekte von Saccharin auf die Zuckervergärung — durch Antiport des Intensivsüßstoffs in die Bakterienzelle im Austausch gegen Lactat — beteiligt ist.
    Notes: Summary In a 1-octanol/phosphate buffer system, saccharin was much more lipophilic than would be inferred from its dissociation constant which, however, determined the partition behavior of acesulfame and cyclamate. The uptake of saccharin into Streptococcus mutans led to a 30 to 40-fold higher concentration of this intense sweetener within cells than in the incubation medium. Acesulfame and cyclamate were distributed between cells and medium essentially in a diffusioncontrolled manner. The uptake of saccharin into S. mutans was found to depend strongly on simultaneous sugar fermentation, and in addition, on external pH, sweetener concentrations, and cell densities. Without glycolysis, caused, for example, by an exhaustion of added sucrose, too acidic external pH, or the addition of glycolysis inhibitors, the uptake of saccharin was diffusion-controlled as in the case of acesulfame and cyclamate. The uptake of saccharin was inhibited by a reversal of the direction of the lactate gradient from in → out to out → in. The activation energy of saccharin uptake into glycolyzing S. mutans was near 18 kJ/mol, while glycolysis itself required 82–98 kJ/mol as activation energy, depending somewhat on experimental conditions. Up to 100 attomol of saccharin per bacterial cell was observed. It was concluded that the cytomembrane of S. mutans was involved in mediating the inhibitory effects of saccharin by an antiport of saccharin into cells in exchange for lactate.
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    Journal of plant growth regulation 7 (1988), S. 1-17 
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    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Purified fractions of soluble proteins from barley leaves have been shown to contain specific binding sites fortrans-zeatin, a natural cytokinin. Such binding is very strong in vitro in concentrated solutions of some salts (ammonium sulfate or potassium phosphate) with optimum at pH 7–8 and temperature within the range 0–20°C. The cytokinin-binding sites have high affinity for zeatin (Kd∼1.5·10−8 M) and low capacity corresponding to 1–1.5 pmol zeatin per milligram of initial soluble protein. Cytokinin binding is reversible; it is due to protein (or proteins) with molecular weight 40–45 kDa. This protein(s) does not bind3H-adenine and3H-abscisic acid. The ability of various compounds to displace3H-zeatin from its high-affinity binding sites is in strict accordance with their biological cytokinin activities. Other phytohormones as well as fusicoccin do not displace3H-zeatin from its binding sites. Specific zeatin binding is sensitive to heat, alkali, and pronase, but not to RNase treatment. The 150- to 200-fold purification of cytokinin-binding proteins was achieved by a combination of ammonium sulfate precipitation and Ultrogel AcA-54- and DEAE-cellulose chromatography. The zeatin-binding protein(s) from barley leaves is suggested to take part in cytokinin action in vivo.
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    Journal of plant growth regulation 7 (1988), S. 37-44 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of [(N-phenyl-N′-1,2,3-thidiazol-5-ylurea)] (thidazuron) on sprouting of potato (Solanum tuberosum L.) tubers and the role of ABA in bud break and subsequent bud growth were studied. Abscisic acid (ABA) was quantitated by enzyme-linked immunosorbent assay (ELISA) from the peel of potato tubers. The ELISA results were also validated by gas chromatography-electron capture detector and confirmed by gas chromatography-mass spectrometry and by a lettuce hypocotyl bioassay. The degree of rest in the tubers was associated with ABA content in the peel. Basal portion (where tuber was attached to mother plant) contained the highest amount of ABA. Thidiazuron reduced ABA content and induced potato tuber sprouting. Exogenously applied ABA stimulated growth of buds that had emerged from dormancy.
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    Journal of plant growth regulation 7 (1988), S. 19-26 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract XE-1019 [(E)-1-(4-chlorophenyl)-4,4,-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol] was injected into bean plants (Phaseolus vulgaris L. ‘Black Valentine’) at doses of 0.1–1000 μg/plant and caused reduced height growth, fresh weight, and leaf area 7 days after treatment. The sprout growth of California privet (Ligustrium ovalifolium Hassk.) was inhibited 52% by 10 μg and growth was further suppressed as the dose was increased to 100 μg, without injury. The shoot growth of American sycamore (Platanus occidentalis L.) and yellow-poplar (Liriodendron tulipifera L.) was progressively inhibited after 3 months as the injected dose of XE-1019 was increased from 2.5 to 240 mg/tree. Neither species was injured. Growth of 1-year-old trees of ‘Golden Delicious’ apple (Malus domestica Borkh) was inhibited 28 days after injecting the stem with 2 mg of14C-labeled XE-1019. At this time, 2% of14C activity has been translocated into the new shoots and 3% was present in the xylem and phloem of the scion. From 96 to 99% of14C-activity found in the xylem and phloem and 92% in the new shoot tissue chromatographed with XE-1019. This indicates that little degradation of XE-1019 occurred during the initial inhibition period.
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    Journal of plant growth regulation 7 (1988), S. 27-36 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relative growth inhibitory activities of paclobutrazol [(2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2(1,2,4-triazol-1-yl)pentan-3-ol]; XE-1019 [(E)-(1-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol]; flurprimidol [α-(1-methylethyl)-α-[4-(trifluoromethyloxy)-phenyl]-5-pyrimidine-methanol]; and triadimefon (a fungicide) [1-(4-chlorophenoxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1-yl)-2-butanone] were evaluated and compared by treating the root zone of young greenhouse-grown tissue-culture-propagated ‘Gala’ (Malus domestica Borkh.) trees. At 0.25 mg/plant, only XE-1019 significantly reduced new stem length and number, area, and dry weight of leaves after 115 days. Paclobutrazol and flurprimidol both significantly reduced growth compared to controls when applied at 0.5 mg/plant, but XE-1019 was more effective. All three gibberellin (GA) biosynthesis inhibitors effectively retarded growth at a dosage of 1 mg/plant. Triadimefon applied at 10 mg/plant had essentially no effect on growth, but at 50 and 100 mg/plant it caused significant but less dramatic growth retardation when compared with the GA inhibitors. Major differences in effectiveness among the triazole GA biosynthesis inhibitors may be due to longevity of effect as well as to extent of inhibition.
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    ISSN: 1435-8107
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The specific inhibitor of gibberellin biosynthesis, (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol (paclobutrazol), inhibited germination ofAmaranthus caudatus L. seeds. Addition of gibberellic acid (GA3), 2-chloroethylphosphonic acid (ethephon), or 1-aminocyclopropane-1-carboxylic acid (ACC) effectively antagonized inhibition. Ethephon was found to be the most efficient antagonist. The transfer of seeds after 1 day's incubation in paclobutrazol to solutions of GA3 or ethephon reversed the inhibition, the effect increasing with increasing concentration of GA3 or ethephon. Seeds incubated in paclobutrazol for 5 days decreased sensitivity to GA3 and ethephon.
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    Journal of plant growth regulation 7 (1988), S. 67-75 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Triapenthenol or RSW 0411 (B(-cyclohexalmethylene)-gamma-(1,1-dimethylethyl)-1H-1,2,4-triazole-1-ethanol), a triazole plant growth regulator, applied preemergence as a separate broadcast application, protected tolerant and midtolerant soybean cultivars from metribuzin (4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one)-induced necrotic injury, and stabilized seedling fresh weight and dry weight loss to herbicide treatment. Soybean yields were not significantly reduced by triapenthenol treatment alone, but 1.12 kg ai/ha metribuzin and a 0.56 kg ai/ha triapenthenol plus 1.12 kg ai/ha metribuzin combination reduced crop yield averaged across cultivars.
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    Journal of plant growth regulation 7 (1988), S. 95-109 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to verify if epidermis integrity played a determinant role in epicotyl elongation induced by fusicoccin (FC), buffers at different pH's, and indoleacetic acid (IAA), we studied the short-term kinetics of elongation growth, the increase of fresh weight in long-term treatment, and the H+ excretion in intact, abraded, and peeled azuki bean epicotyl sections. We demonstrated that the epidermis is more sensitive to IAA, whereas the cortex is highly responsive to protons. Our data are consistent with the “acid growth theory.” In addition, our studies support the idea that the epidermis may be the tissue target for auxin, but its integrity is necessary for IAA-induced elongation.
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    Journal of plant growth regulation 7 (1988), S. 111-119 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Factors affecting the induction of ethylene synthesis in apple were investigated through their effect on the delay in ethylene production (DEP) in single fruits after harvest. DEP in Cox apples was not affected by temperature from 1 to 25°C, but in Golden Delicious, DEP was shorter below 12°C and above 20°C. An orchard treatment with daminozide increased DEP in apples held below 15°C or treated with ethylene after harvest. Experiments in which the temperature was changed after ethylene treatment showed that ethylene was less active at 5°C than at 15°C but that response to it could be equally rapid. The results suggest that endogenous ethylene is not regulatory prior to the onset of rapid ethylene synthesis.
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    Journal of plant growth regulation 7 (1988), S. 145-151 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ent-kaurene (ent-kaur-16-ene) and squalene were analyzed in extracts of the shoots of three cultivars of rice (Oryza sativa L.) of 14 and 28 days of age by gas chromatography-mass spectrometry (GS-MS) and GC-selected ion monitoring (GS-SIM).Ent-kaurene occurred at approximate concentrations of 〈1 to 13 ng/g f.w. in 14-day-old plants and 26 to 147 ng/g f.w. in 28-day-old plants. Shoots of the dwarf cultivar Waito-C contained much lessent-kaurene than the other two cultivars at both developmental stages. The level ofent-kaurene in the dwarf cultivar Tan-ginbozu was similar to that in the normal cultivar Nihonbare at 14 days but was lower than in Nihonbare at 28 days. Trace amounts ofent-isokaurene (ent-kaur-15-ene) were also found in the extracts of all three cultivars of shoots at 28 days. Squalene occurred in approximate concentrations from as low as 19 ng/g f.w. in 28-day-old Waito-C shoots to as much as 626 ng/g f.w. in 14-day-old Nihonbare shoots. Both Tan-ginbozu and Waito-C shoots contained less squalene than Nihonbare shoots at both developmental stages.
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    Agriculture and human values 5 (1988), S. 3-5 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Agriculture and human values 5 (1988), S. 24-34 
    ISSN: 1572-8366
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A farming systems approach to development has meant many things over the past 15 years, depending on its institutional and ecological setting, its target populations, and the goals motivating its implementation. Despite the diversity of approaches, and the sometimes rancorous discussion over which was best and why, the approach is now recognized in many places as the only one that can identify and respond to the needs of limited resource farm families, especially those in marginal ecosystems. Involving an iterative process of diagnosis, design, testing and extension, the farming system approach to date has done more to change research objectives at national and international institutions than to change actual farmer practices. By legitimizing what limited resource farmers do and why they do it, a farming systems approach lends itself to policy analysis as well. Recent research in farming systems suggests greater attention should be payed to exogenous variables, including policy and infrastructure, as well as to development of technology that really responds to the felt needs of limited resource farmers in improving their level of living.
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    Agriculture and human values 5 (1988), S. 6-15 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper presents some of the basic trends, issues, and questions regarding the last four decades of international development cooperation in agriculture. The impact of technical cooperation tends to account for only a small proportion of change; the bulk of the variance being caused by internal, rather than external, forces and events. The paper reviews both multilateral and bilateral technical cooperation, and then illustrates with the case of U.S. universities in international technical cooperation. It goes on to question the difference between “development” and “merely change,” and asks who are the real beneficiaries? Finally, the paper suggests the following factors affecting continuity and change as forces to be analysed with respect to any attempt at technical cooperation: biological, physical, cultural, social, economic, administrative, political, and diplomatic. The world experience of the past four decades confirms that without consideration of such a human ecology of continuity and change, well-meaning interventions in international technical cooperation are likely to have unintended consequences for both “donors” and “recipients.”
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    Agriculture and human values 5 (1988), S. 35-48 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract International agencies have contributed significantly to the promotion of capital-intensive fisheries development programs in many Third World nations. Activities of both bilateral and multilateral development assistance agencies are examined and shown to have certain common features, notably production-oriented programs typified by the introduction of powerful new fishing technologies, and the promotion of fishery exports as a means of increasing foreign exchange earnings. The argument is advanced that these programs have been largely detrimental to the best interests of recipient nations because they have ignored both resource limitations and the distributional consequences of such development. Fisheries development programs in the Third World are seen as being shaped by a convergence of institutional and class interests between national and international agencies. The perspective of political economy is used to examine these interests and explain their relation to policy outcomes. Evidence is presented to show that international agencies have contributed to dualistic patterns of industry growth which have skewed development benefits towards a narrow urban elite. Rural small-scale fishers have been increasingly marginalized as a result of their inability to compete over a limited and, in some cases, depleted resource. Fisheries development and resource management need to be seen as complementary aspects of a single process rather than as separate activities. Central to fisheries management is the question of resource allocation between competing users. Suggestions are offered by which international development agencies can play an important role in encouraging resource use patterns which are both biologically sustainable and socially just.
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    Agriculture and human values 5 (1988), S. 16-23 
    ISSN: 1572-8366
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Technology transfer is a multi-level process of communication involving a variety of senders and receivers of ideas and materials. As a response to market failure, or as an effort to accelerate market-driven social change, technology transfer may combine public and private aparatus or rely solely on public institutional mechanisms to identify, develop, and deliver innovations and information. Technology transfer institutions include universities, government ministries, research institutes, and what may be termed the ‘project sector’. Four farm- and village-level change models are considered: traditional community development, adoption-diffusion, training and Visit Extension, and Farming Systems Research. The challenges to technology transfer efforts center on developing indigenous capacity to generate and adapt agricultural technology to local conditions. This is the primary objective of technology transfer in agriculture and the basis for advancing rural development.
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    Agriculture and human values 5 (1988), S. 49-60 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Social forestry, in contrast to traditional forestry, is intended to meet biological/environmental, procedural and equity goals. Social forestry projects may not fulfill this multiplicity of goals either because priority is given to a single goal or because various factors including the structure and norms of implementing institutions and the distribution of local power overwhelm procedural and distributive intentions. Thus, despite participatory and equitable project designs, social forestry projects may result in the distribution of benefits to the rich and costs to the poor and products that either have little local value or lose their value over time. Factors leading to these outcomes are explored and countervailing measures considered.
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    Agriculture and human values 5 (1988), S. 61-68 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract There is in much of contemporary irrigation development a large paradox. Irrigation development, while contributing to productivity, frequently thwarts social justice—it either introduces incipient inequalities in access or it reinforces those that exist. More progressive irrigation development policies are being developed and tested in several countries. These new approaches give more attention to the role of local groups in governing irrigation systems while reducing the heavy-handed involvement of state agencies. These new approaches are seen as both consistent with achieving greater fairness in irrigation development as well as with the new fiscal realities of governments nearly everywhere. Supporting these new approaches are a set of institutions and individuals with nonengineering expertise who traditionally have not been defined as central to irrigation development.
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    Agriculture and human values 5 (1988), S. 92-100 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study investigates the socio-historical relationships existing between the development of the agricultural structure and the process of technical development. Adopting a political economy posture, it is argued that the development of technical procedures in agriculture has been aimed historically at the maximization of production and productivity. This phenomenon has been generated by the social hegemony of groups interested in the enhancement of accumulation of capital and has been translated into an emphasis on large productive units, which discriminates against small and medium farms. This pattern of development has been legitimized through a social consensus which involves an emphasis on small family producers. This contradiction is embodied in the generation of a dualistic agricultural structure characterized by the prosperity of large farms, the persistence of small farms and the crisis of medium farms and in patterns of overproduction and unavailability of food for the poor segments of society.
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    Agriculture and human values 5 (1988), S. 69-76 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this paper, the author points out that although by-passed by international development assistance in many parts of the world, women have been providing skill and labor for agricultural production, as well as subsistence of food, water and firewood for their families. Some of the assumptions which have contributed to the marginal attention to women as agriculturalists in international development assistance programs are reviewed. Factors contributing to these assumptions, examples of achievements in development projects and persistent problems are discussed. Some issues which continue to challenge national policy makers in developing countries as well as donors are identified. The author notes that while various donors and organizations address bits and pieces of the issues intermittently, a systematic, organized and universally shared approach to the issues, within a nation state or on a global basis is lacking. She concludes that just as agricultural production inputs and information need to be modified to fit the unique constraints of any ecological niche, similarly, modifications must be made in development assistance programs to fit the specific needs of women in their agricultural roles and cultural settings.
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    Agriculture and human values 5 (1988), S. 101-111 
    ISSN: 1572-8366
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Agricultural research raises fundamental ethical and value questions going beyond those in other fields both because of its public funding and because its results have significant impacts on habitats and other species. Questions about the sustainability of modern agriculture, which are shared with other sectors, require us to examine alternative visions and structures. These can be seen to range from status quo preserving ideologies to change-oriented utopias. It is argued that at the national level current ideologies—which include positivistic approaches and belief in the neutrality of technology—mask real structural and policy choices as well as their ethical and value implications. At the international level, the export of fossil-fuel based modes of agriculture to the developing countries raises additional structural, policy, value, and ethical issues.
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    Agriculture and human values 5 (1988), S. 77-91 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The first part of this paper discusses five sets of forces that have had a major influence on world agriculture in the post-World War II period. These include (1) high rates of population growth in the developing countries; (2) a steady increase in economic integration world-wide, driven by technological breakthroughs in the communication and transportation sectors; (3) major realignments in the values of national currencies; (4) growing distortions in economic policies in both the industrialized and developing countries; and (5) growing diffusion of new production technology from the industrialized to the developing countries. The second part reviews the changing role of international development assistance in support of agriculture in light of these historical forces. Such assistance successively stressed the development of extension services, food aid, institutional development of higher level education institutions, the development of research capacity, and rural development. A look to the future is the subject of the third part of the paper. This includes a discussion of the difficulties in sustaining U.S. foreign assistance, especially when that nation is letting its own economic house fall into such disarray.
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    Agriculture and human values 5 (1988), S. 1-2 
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    Agriculture and human values 5 (1988), S. 112-123 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper uses a cultural ecology of development approach to critique existing models of development. The critique identifies existing models as running counter to ecological and biological imperatives, placing an over-emphasis on growth as the solution to development, and resulting in considerable cultural wastage. An argument is made that many of the attempts to construct an alternative development paradigm can be grouped within the cultural ecology of development approach. Ten precepts that will enhance the long-term survivability of the earth are proposed. The final portion of the paper looks at how compatible current trends in thinking about development are with these ten precepts and assesses the prospects for adoption of these precepts in an alternative development paradigm.
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    Agriculture and human values 5 (1988), S. 26-35 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper argues that the new biotechnologies will affect the natural environment primarily in two ways: by bringing relatively “wild” areas, such as forests and estuaries, under domestication, and by forcing areas now domesticated, such as farms, out of production, because of surpluses. The problem of the safety of biotechnology—the risk of some inadvertent side-effect—seems almost trivial in relation to the social and economic implications of these intentional uses. The paper proposes that we should be more concerned about the successful uses of biotechnology than about the possible mishaps or failures.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In periods of social crisis, policymakers become particularly vulnerable to interest groups mobilizing to compete for scarce funds. At this point, legislators are no longer able to address the specific needs of their primary constituency directly, but rather are forced to do so in pretext only. New, unfamiliar technologies provide ample ammunition for astute interest groups to take advantage of times of economic turmoil and maneuver for policy support through dramatic campaigns of “salesmanship.” By publicizing a crisis situation, dramatizing it effectively, and advertising an innovation as the solution to the crisis, legislators may be effectively persuaded to give priority to interest group pressures above and beyond those of the local constituency. Iowa's attempts to address the farm crisis through economic development strategies relying on biotechnology is examined in this paper. The results of extensive surveys of Iowa's legislators and farmers are examined and the consequences for Iowa's policy process of using biotechnology under the auspices of economic development are discussed.
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    Agriculture and human values 5 (1988), S. 36-52 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the voluminous literature on the subject of bGH we have yet to find an attempt to frame the issue in specifically moral terms or to address systematically its ethical implications. I argue that there are two moral objections to the technology: its treatment of animals, and its dislocating effects on farmers. There are agricultural biotechnologies that deserve funding and support. bGH is not one of them.
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    Agriculture and human values 5 (1988), S. 71-95 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Agriculture and human values 5 (1988), S. 61-70 
    ISSN: 1572-8366
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract All biotechnology-related promises are based upon its technological potential; yet, many of these promises assure the solution for chronic socio-economic problems in the Third World through a new technological revolution in agriculture. The forecasting is that such a revolution will start delivering its most profound impact early in the 21st century. However, 11 years before the year 2000, a critical analysis of its promises against its current trends indicates that the future use and impact of biotechnology in the Third World rely presently upon crucial contradictions. As a result of such contradictions—Social Goals vs. Private Gains, Social Problems vs. Technical Solutions, Agricultural vs. Industrial Revolution, Cooperation vs. Competition, and Control over Nature vs. Control Over People—there is a high likelihood that (1) traditional farming will become increasingly obsolete, (2) technological and economic dependence of developing on developed countries will persist and even increase, (3) food and fiber production will be increasingly dislocated from developing to developed countries and from farms to industries, (4) the market for specific tropical products will be destroyed and therefore entire economies may collapse, (5) hunger and poverty will persist and even increase, and (6) social unrest may increase worldwide.
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    Agriculture and human values 5 (1988), S. 3-14 
    ISSN: 1572-8366
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reviews current trends in the development of agricultural biotechnology, including (1) the recent and potential biotechnology products and processes in the plant, animal and food sciences, and (2) the enormous increase in Federal and State government and industrial investments in biotechnology research. Next we analyze the impacts and possible consequences of agricultural biotechnology for public and private agricultural research and for the structure and nature of the food system in this country and around the world. We conclude with a range of proposals for agricultural research policies. Among the possible consequences we discuss are: (1) a shift in disciplinary emphasis in the research community to molecular biology, (2) reduction of research on systems, ecology, and the social sciences, (3) increased concentration of research funds at a small number of institutions, (4) reduction of long-term research in the public sector, (5) increased collaboration between industry, government and universities with a restriction of scientific communication, and a potential for conflict of interest, favoritism and increased scientific misconduct, (6) a change of the primary goals and agenda of the public sector research community, and (7) increased concentration in the agribusiness sector and the industrialization of the food system. Our policy suggestions include: (1) maintaining and strengthening an independent public research and teaching system, (2) striking a balance between short-term proprietary biotechnology and long-term nonproprietary research, (3) maintaining an extension system that delivers biotechnology information and products to all potential users, (4) developing a regulatory system that adequately protects the public and provides clear guidelines to industry. (5) assisting developing nations to reap the benefits of the biotechnology revolution, and (6) establishing mechanisms to foster broad-based understanding of the social and ethical issues relating to agricultural biotechnology and to promote research on the social and ethical impacts.
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    Agriculture and human values 5 (1988), S. 96-98 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Agriculture and human values 5 (1988), S. 1-3 
    ISSN: 1572-8366
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Agriculture and human values 5 (1988), S. 53-60 
    ISSN: 1572-8366
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The “new” agricultural biotechnologies are presently high-priority items on the national research agenda. The promise of increased efficiency and productivity resulting from products and processes derived from biotech is thought to justify the commitment to R&D. Nevertheless, critics challenge the environmental safety as well as political-economic consequences of particular products of biotech, notably, ice-nucleating bacteria and the bovine growth hormone. In this paper the critics' arguments are analyzed in explicitly ethical terms, and assessed as to their relative merits. In some cases, a principle of “do no foreseeable harm” as well as a clear determination of likely harms would force us to conclude that research, development, and diffusion of a product or process derived from biotechnology is ethically wrong. In all cases, one conclusion that can be reached is that everyone involved in research, development, marketing and adoption of biotech products is responsible for the results of their actions; thus, each individual has a responsibility to consider a broader range of values and goals that effect and are effected by the biotechnology effort.
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    Agriculture and human values 5 (1988), S. 4-15 
    ISSN: 1572-8366
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Agricultural research and education ended 100 years of funding under the Hatch Act with a decade of unprecedented criticism of goals and outcomes. This paper examines the way that planners can accommodate some of these criticisms within a framework for understanding the ethical and social goals of agriculture that is consistent with traditional practice. The paper goes on to state that some criticisms are so fundamental that they cannot be readily incorporated into this framework. They must be regarded as a challenge, both politically and intellectually, to longstanding practices within academic institutions devoted to agriculture.
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    Agriculture and human values 5 (1988), S. 26-33 
    ISSN: 1572-8366
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Colleges of agriculture are being forced to adapt to a changing world. The forces behind these changes affect all departments within the college. In this paper, the place of agricultural economics within the college and within the university is identified, the current situation facing the discipline is outlined, and strategies for responding to the forces of change are discussed. Three alternatives are available: continuation, termination, and metamorphosis. Different departments are likely to pursue different strategies. Some may disappear altogether or may be absorbed into the parent discipline, economics, or into the business school. Other departments may transform themselves into specialized sub-disciplines, such as natural resources or agribusiness departments. Still others may continue with little or no change. Whatever course is followed, strategic planning will surely be necessary.
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    Agriculture and human values 5 (1988), S. 16-25 
    ISSN: 1572-8366
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This essay examines the growing concerns about disciplinary narrowing occurring in agricultural research and the prospects of ameliorating the detrimental effects of disciplinary compartmentalization while capitalizing on its positive effects. The general model for agricultural science is that disciplinary groupings set the logic and standards for research; the disciplinary sciences are set in a hierarchical arrangement which allows communication from the relevant basic sciences through applied research into technology development and use and problem-solving. But agricultural research throughout most of its history has been goal-oriented and, therefore, is subject to ethical judgements of its worth and consequences. Also, strategic aspects of agricultural research have been subject to the evaluations and criticisms of both scientists and critics with differing interests at stake. Goals can change and organizations can be set to enable multidisciplinary and interdisciplinary research, but both goals and organizations come up against values associated with the disciplinary quality of the research, the social setting of academic science, the competition for resources, and the scientific reward system. However, there are changes underway in the agricultural scientific community which may recast the impacts of disciplinary structuring: (1) changes in the disciplinary components of subject areas and departments, (2) evolution and introductions of integrative and systems sciences into the system, (3) infusion of the same new powerful tools into most of the sciences, and (4) increased networking among scientists of different disciplines. Given that scientists' values and personalities intrude in agricultural science and research strategies, the future of agricultural research may rest on the scientists' intellectual vision and philosophical awareness that go beyond the expected disciplinary limits.
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    Agriculture and human values 5 (1988), S. 34-39 
    ISSN: 1572-8366
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reports the results of a survey of the teaching of courses in agricultural history in the seventy-four Land Grant institutions in the United States and its territories. It concludes with the expression of concern that the subject matter, agricultural history, is nearly a dying field, and only heroic measures will succeed in rescuing it.
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  • 50
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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    Agriculture and human values 5 (1988), S. 50-56 
    ISSN: 1572-8366
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Traditionally, the Swedish Agricultural Extension Service has delivered technical information to farmers with the aim of increasing productivity and efficiency in farming. Present problems with overproduction of food and the negative social and environmental consequences of present farm practices has brought this traditional mission in question. In a situation of budgetary constraints it has been suggested that the funding of the governmental Agricultural Extension Service should be cut down or even discontinued altogether The article argues that this would be a mistake. The various negative consequences of modern agriculture indicate that we are far from an ideal mode of agricultural production. Instead, public opinion and new guidelines for agricultural and environmental policies call for substantial changes in Swedish agriculture with respect to pollution, preservation of non-renewable resources, maintaining an open rural landscape, ethical aspects of animal production, rural development etc. This reorientation of Swedish agriculture presumes that decision-makers, farmers, and the public at large get an opportunity to learn more about the complexities of agricultural production. In contributing to this learning process the Agricultural Extension Service would have an important mission. To be able to fulfill this mission, extension professionals must be provided an opportunity to learn a broader concept of productivity and efficiency in agriculture, for instance, how to extend cost-benefit analyses and technical criteria of efficiency to include social, environmental, and ethical aspects. Our present extension staff has not received adequate training for this task. It is suggested that all agricultural colleges need to create departments of Rural Sociology and Agricultural Humanities to provide agricultural students and professionals an opportunity to develop a better understanding of agriculture and make them prepared to take on the challenges and responsibilities they confront in developing our future agriculture.
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  • 52
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Difficulties in getting participants in agricultural research policy disputes to work fairly with four different and sometimes conflicting normative viewpoints might be lessened by attending to the deeper cultural differences that lie behind differences of normative view. Mediation of policy disputes might work better if cultural differences were better understood and described impartially. By treating deep differences as ideological, in a non-pejorative sense, descriptions can forestall impulses to combat, improve communication, and open fresh prospects for compromise without attempting to change people's basic thinking.
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    Agriculture and human values 5 (1988), S. 84-91 
    ISSN: 1572-8366
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The author describes a team taught (philosophy/agriculture) professional ethics in agriculture course at Cal Poly, San Luis Obispo. He shows how the teaching, student selection (half agriculture, half non-agriculture), topic selection and class projects (mock public forums on critical issues aimed at achieving a public consensus) were chosen to achieve one main goal, a professional ethics aimed at public service. A second goal, public awareness of the legitimate needs of agriculture, is pursued simultaneously. The public-good orientation of this model of professional ethics is distinguished from the frequent professional-defensive role found in other fields. The author indicates that agriculture's need for publicly effective and credible leadership makes the older defensive models useless today as well as wrong, given the common-good goals of ethics. A short list of goals of agriculture is proposed: sufficient, sustainable and healthy food/fiber supplies by means which respect the dignity of all participants. From these goals it is claimed that consensus “first principles” of agricultural ethics can be constructed. More fundamental first principles, such as the golden rule, are given their appropriate role. From these some simple criteria for the problem solving efforts of the students are suggested: broad consideration of technical and human impacts (no dismissal of impacts as “externalities”), awareness of the need for technical information, consideration of risk-reducing alternative tools or policies, and fair, humane and consistent risk/benefit analysis. The author concludes with a description of the impact on students, the importance of the role model of agriculture teachers in the course with the resultant importance of the team-taught format, and the need for special administrative support to attain the goals of the course.
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    Agriculture and human values 5 (1988), S. 92-95 
    ISSN: 1572-8366
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a number of surveys, Canadian farmers have found the absence of information to be a major obstacle to the development of sustainable agriculture. The traditional sources of information for farmers have been unable to provide them with suitable information. One reason for this deficiency is the absence of suitable training for agriculture professionals. The details of a newly created course designed to address these problems at the Faculty of Agriculture of McGill University are provided, and some suggestions made about the pedagogy and course content of an entire program.
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    Agriculture and human values 5 (1988), S. 40-49 
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    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Agricultural and rural development programs can only succeed if they are based on effective participation and support of actors from the policymaking stage through all levels, including field personnel and primary producers. But these actors must possess the knowledge, attitude, and skills necessary to execute their tasks. For this reason, agricultural education and training has an integral part to play in any agricultural and rural development effort. The Food and Agricultural Organization (FAO) of the United Nations has made significant contributions to the development of agricultural education in its member countries. FAO's contributions have focused on institution building, group training, and fellowship/study tours. This paper reviews some of the lessons learned through FAO involvement in these areas and recommends directions for improvement. These recommendations include the following: 1. Higher agricultural education institutions should become more involved in rural development by participating in appropriate activities leading to the formulation of national development policies. 2. These institutions should develop education programs and curricula responding to the need of development by providing the knowledge and skills required to meet the needs of the people concerned and to solve their technical and socio-economic problems. 3. More emphasis should be placed on improving the quality of teaching and on practical training. 4. The same institutions should undertake to play a leading role in the agricultural and training systems at the national level. 5. Research should be an integral part of higher educational institution programs and the research themes should include interests of small and poor agricultural producers and measurement of research impacts. 6. Regional and inter-regional collaboration among higher agricultural education institutions should be encouraged.
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    Agriculture and human values 5 (1988), S. 96-101 
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    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract If the field of agricultural ethics is to realize its potential and if the agricultural and philosophical communities are to address the impending changes in world food production, there is a need for education in public, governmental, and academic arenas. The development of a symposium on agriculural ethics is an effective method for “raising awareness” of the imminent need for a consolidation of philosophical and agricultural expertise. Based on experience, a series of organizational guidelines and their associated philosophical issues are presented. The initial step is a determination of the purposes of the symposium, which leads to the consideration of the choice of speakers and topics. The best series of speakers will ultimately prove to be ineffectual if the logistics of the symposium are not carefully planned. The scheduling, format, timing and location of the talks are critical. Related to these practical aspects is the organization of promotional efforts. Since one of the goals is, presumably, to enlighten a target audience, effective promotion should be a central concern. Underlying all of these considerations is the budget. How the agricultural ethics symposium at the University of Wyoming addressed these issues is presented, with a critical review of our efforts based on external evaluations.
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    Biology and fertility of soils 5 (1988), S. 276-281 
    ISSN: 1432-0789
    Keywords: Metolachlor ; Biodegradation ; Soil perfusion ; Microbial metabolites ; Dechlorination ; Actinomyces spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Degradation of the herbicide metachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide] was studied in a soil perfusion system. After 28 days of perfusion, the 14CO2 evolved from a Virginia soil (soil A), which had been previously treated with metolachlor (Dual) for 5 years, accounted for 18.4% of the added 14C-metolachlor, while only 3.5% of the 14C was liberated as 14CO2 from a soil of the same field which had no history of Dual treatment (soil B). No 14CO2 was liberated from γ-irradiated soil A. After incubation, metolachlor constituted almost all the extractable 14C in sterile soil A, while about 20% of the added 14C extracted from non-sterile soil A consisted of products of metolachlor; 14.8% was identified as dechlorinated metolachlor. No mineralization occurred in actinomycete-inoculated sterile soil A, but 30% of the added 14C was recovered in the form of transformation products of metolachlor. Our results demonstrate clearly that microbial activity is responsible for the mineralization of metolachlor, and that degradation is enhanced in herbicide-acclimated soils.
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    Biology and fertility of soils 5 (1988), S. 295-298 
    ISSN: 1432-0789
    Keywords: pH measurement ; Rhizosphere ; SB electrodes ; Proton secretion of roots ; Red clover ; Rhizotrone ; Rhizobium spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Measurements of pH were made at the root surface of Trifolium pratense, using Sb electrodes. Nodulated plants were grown in rhizotrones on a sandy soil free of carbonate and on a clay soil rich in carbonate. In the sandy soil, pH at the surface of root laterals was about 1 unit lower than in the bulk soil. The lowest pH values were found at the root tips. In the calcareous soil, pH measured at the root surface did not differ from pH in the bulk soil. This soil had a much higher H+ buffer capacity than the sandy soils. It seems likely that H+ ions excreted from the roots grown in the calcareous soil were directly neutralized by soil carbonate.
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  • 59
    ISSN: 1432-0789
    Keywords: Organic soil P ; Phosphatase ; P uptake - ; Trifolium alexandrinum ; Hordeum vulgare L. ; Avena sativa L. ; Triticum aestivum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficiency of phosphatases produced by clover, barley, oats and wheat was investigated in soils treated with sodium glycerophosphate, lecithin and phytin. Root exudates of aseptically grown clover were also examined for the breakdown of different organic P compounds in order to test the efficiency of plant-produced phosphatases. In general, the plants were able to use P from all the organic sources used in the study almost as efficiently as inorganic sources. Dry-matter yield, P uptake, acid and alkaline phosphatase activity and microbial population were increased in all the P treatments. Organic P enhanced alkaline phosphatase activity. Lecithin increased fungal, and phytin bacterial growth. There was no alkaline phosphatase activity in the asepticallly grown clover root exudates. Phosphatase released in aseptic culture after 4 weeks of clover growth was able to efficiently hydrolyse sodium glycerophosphate, lecithin and phytin. The amount of organic P hydrolysed in this and in the soil experiment surpassed plant uptake by a factor of 20. This suggests that the limiting factor on plant utilization of organic P is the availability of hydrolysable organic P sources.
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    Biology and fertility of soils 5 (1988), S. 323-327 
    ISSN: 1432-0789
    Keywords: Earthworm ; Aporrectodea rosea ; Axial pressure ; Tunnelling ; Burrowing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The axial forces generated by the earthworm Aporrectodea rosea were measured by directing the earthworm to tunnel into soil discs mounted on an electronic balance connected to a datalogger. The area over which the force acted was estimated from the size of the hole created by the tunnelling. The maximum force recorded by an individual worm was 0.760 N and the mean maximum value was 0.465 N. The maximum pressures recorded was 116.5 kPa, while the mean maximum pressure was 72.8 kPa. These axial pressures are approximately one-tenth of the maximum axial pressure recorded for plant roots. The statistical distribution of activity as the worms tunnelled through the soil discs was analysed. Pressures recorded were well in excess of physiologically measured pressures and the generation of forces within muscles to achieve these pressures was calculated by some assumptions. It was estimated that there is a maximum tension in the circular muscles of 0.15 N per segment of the worm.
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    Biology and fertility of soils 5 (1988), S. 333-337 
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhiza ; Greece ; Glomus ; Degree of root infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This paper reports the results of a survey of the occurrence of vesicular-arbuscular mycorrhizas and spores of the associated fungi in a variety of soils in Northern Greece. The roots of several crop species were examined for the presence of mycorrhizal structures and all samples showed evidence of infection, though to varying degrees. Cotton plants, in particular, had heavy arbuscular infection. Intact and presumably viable spores were relatively infrequent and empty non-viable spores were much more common. Soil characteristics at each sample site are reported also, but only broad generalizations are made at this stage with respect to correlations between mycorrhizal occurrence and edaphic factors.
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    ISSN: 1432-0789
    Keywords: Trifolium pratense ; VA mycorrhiza ; Glomusfasciculatum ; Inorganic P fractionation ; Soil sterilization ; Mycorrhizal interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five selected vesicular-arbuscular mycorrhizal (VAM) fungi and the native population of a cambisol were tested in sterilized soil conditions, with Trifolium pratense as host plant. Indigenous fungi were the most effective in enhancing plant growth and P uptake, which were correlated with a higher root colonization. Selected fungi did not spread further in the root after 4 months from sowing, occupying less than 10% at the end of the experiment; inoculation with Glomus fasciculatum E3 yielded a higher dry-matter production than any other VAM species, but did not significantly increase shoot P concentration above that of the non-mycorrhizal control. Interactions between indigenous and introduced VAM fungi were studied in unsterilized soil. Results from fresh and dry weights of shoots and the percentage of fungal infection showed that the native endophytes competed more efficiently in colonizing the root. Inoculation with selected VAM species did not improve plant growth. Sterilization altered the inorganic P fractions of the soil, particularly those extracted with NH4F and NaOH. Sterilized soil contained less inorganic P than unsterilized soil, but more soluble P. By the end of the experiment in sterilized soil, P extracted with NH4Cl, NH4F and NaOH and total inorganic P were significantly different among inoculation treatments, suggesting that VAM fungi may differ in their ability to take up P.
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    Biology and fertility of soils 6 (1988), S. 68-72 
    ISSN: 1432-0789
    Keywords: Soil enzymes ; Urease ; Determination of ammonium ; Storage conditions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A rapid assay for soil urease in the absence of bacteriostatic agents has been developed. The method comprises incubation of soil with an aqueous or buffered urea solution, extraction of ammonium with 1 N KCl and 0.01 NHCl and colorimetric NH4 + determination by a modified indophenol reaction. The method is characterized by high sensitivity and stability of the coloured complex formed. Measurements obtained by this method showed that no change in urease activity occurred when field-moist samples of soils were stored at −20°C for as long as 5 months. Air-drying of field-moist soil samples may lead to an increase in urease activity.
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  • 64
    ISSN: 1432-0789
    Keywords: Decomposition ; Mesh bag ; Nitrogen mineralization ; 15N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In two field experiments, plant materials labelled with 15N were buried separately within mesh bags in soil, which was subsequently sown with barley. In the first experiment, different parts of white clover (Trifolium repens), red clover (T. pratense), subterranean clover (T. subterraneum), field bean (Vicia faba), and timothy (Phleum pratense) were used, and in the second, parts of subterranean clover of different maturity. The plant materials were analysed for their initial concentrations of total N, 15N, C, ethanol-soluble compounds, starch, hemicellulose, cellulose, lignin, and ash. After the barley had been harvested, the bags were collected and analysed for their total N and 15N. In the first experiment the release of N was highest from white clover stems + petioles (86%) and lowest from field bean roots (20%). In stepwise regression analysis, the release of N was explained best by the initial concentrations of lignin, cellulose, hemicellulose, and N (listed according to decreasing partial correlations). Although the C/N ratio of the plant materials varied widely (11–46), statistically the release of N was not significantly correlated with this variable. The results of the second experiment using subterranean clover of different maturity confirmed those of the first experiment.
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  • 65
    ISSN: 1432-0789
    Keywords: Rhizobium mefloti ; Glomus fasciculatus ; Medicago sativa ; Succinate dehydrogenase ; Acetylene reduction assay (ARA) ; VA mycorrhiza ; Alfalfa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Alfalfa (Medicago sativa, L. cv Aragón) plants were grown under greenhouse conditions in pots of inert sand and vermiculite. The plants were inoculated with Rhizobium meliloti strain 102F28, with Glomus fasciculatus or with a mixture of both microorganisms. Plants inoculated with both Rhizobium and Glomus had the highest shoot dry weight and the lowest root-to-shoot ratio. Roots from dually inoculated plants also had a higher oxygen uptake and nodule nitrogenase activity than those from plants inoculated with either of the two microsymbionts alone. However, the dry weight of the roots from only VAM-infected plants was higher than those from Rhizobium or from Rhizobium plus Glomus-inoculated ones. These differences did not correlate with succinate dehydrogenase activity, which was similar between treatments. Nutrient element concentrations were increased in dually infected plants in comparison with those of plants inoculated with only Rhizobium or Glomus. These data suggest that Rhizobium may affect fungal metabolism and that the effect is not achieved via the tricarboxylic acid pathway.
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  • 66
    ISSN: 1432-0789
    Keywords: Phytotron study ; Corn plants ; Denitrification ; 15N balance ; N2 flux by 15N method ; N2O flux by gas chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Containers filled with soil mixed with potassium nitrate highly enriched in 15N were planted with corn (Zea mays L.) and kept in a phytotron under controlled conditions for 79 days. Soil water content was normally maintained at exactly 60% water-holding capacity (−33 kPa), but it was increased several times to 85% (−5 kPa) for short periods to favour denitrification. The soil headspace was sealed from the phytotron atmosphere and aerated by a continuous stream of air. Nitrous oxide emission was measured by estimating the N2O concentration differences in the air entering and leaving the containers. Emission of N2 was estimated by mass spectroscopy from changes in the N2 composition in the temporarily enclosed soil headspace. Both methods were carefully checked for accuracy by different tests. At specific times during the experiment the distribution of 15N between plants and soil was determined and a 15N balance established. Emission of N gases peaked at times of increased water content and reached maxima of 149 and 142 μg N pot−1 day−1 for N2O and N2, respectively. While N losses of 5% ± 2% were indicated by the 15N balance, only 1.1% ± 0.3% loss from 2.7 g applied N was estimated from the N2O and N2 measurements after 79 days. Possible reasons for these differences are discussed.
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  • 67
    ISSN: 1432-0789
    Keywords: Spatial nitrogen distribution ; Prilled urea ; Urea supergranules ; Neem cake coated urea ; Submerged rice soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field experiments were conducted on transplanted rice (Oryza sativa L., var. Ratna) in a submerged soil, in order to study the distribution of N applied at 100 kg/ha. N was introduced as prilled urea or neem cake coated urea broadcast on the soil, and as urea supergranules, 1 or 2.5 g in size, point-placed at 5 cm depth. The surface-broadcast N was found mostly in the top 0–5 cm layer of soil and there was only a small vertical movement of applied N to 5–10 cm depth. With point placement of 1-g urea granules, the fertilizer N was found mostly at 5–10 cm depth and within 2.5 cm horizontal distance from the point of placement, compared with 5–10 cm depth and within 5 cm horizontal distance from the point of placement for the 2.5-g granules. With the two broadcast applications, the NH4 +-N content reached peaks of 40–68 mg/kg soil in the top 0–5 cm of soil within the first 3–6 days, decreased at a faster rate from the 6th to 12th day and then at a slower rate up to the 32nd day. In contrast, the NH4 +-N content around the points of placement of the urea supergranules reached peaks of 570–900 mg/kg soil during the first 3–12 days after placement, then decreased rapidly during the next 6–9 days, after which the values remained more or less unchanged but were still higher than the untreated control value.
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  • 68
    ISSN: 1432-0789
    Keywords: Muramic acid ; Glucosamine ; Soil ; HPLC ; Biomass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The optimal release and quantitative estimation of muramic acid and glucosamine were studied simultaneously in soil samples. The effect of hydrolysis conditions, HCl concentration, hydrolysis time, the ratio of soil dry weight to acid, and the recovery of reference substances were investigated. Derivatization of the fluorogenic reagent o-phthalaldehyde, in the presence of 2-mercaptoethanol with the residue of a soil hydrolysate, was achieved by optimizing the relative amounts of o-phthalaldehyde to hydrolysate in the reaction mixture, the pH of both, and the incubation period. A linear relationship was found between the fluorescence response and the concentration of the test substances. The muramic acid, as well as the glucosamine (o-phthalaldehyde) derivatives gave single peaks, and complete separation from interfering substances at the picomol level was achieved in a short time (3 h preparation and 30 min for chromatography) by using high-performance liquid chromatography.
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  • 69
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    Biology and fertility of soils 6 (1988), S. 137-140 
    ISSN: 1432-0789
    Keywords: Rhizobium meliloti ; ELISA ; Growth temperatures ; Competition ; Medicago sativa ; Enzyme-linked immunosorbent assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Axenically grown alfalfa (Medicago sativa L. var. Peace) was simultaneously inoculated with Canadian commercial Rhizobium meliloti strains NRG-185 and BALSAC. The plants were grown for 7 weeks in sealed units at five different root temperatures (8°, 13°, 17°, 21°, and 25°C) and at a relatively constant air temperature (24°–30°C). Nodule occupancy by each strain was determined by enzyme-linked immunosorbent assay (ELISA). Nitrogenase activity, nodule fresh weight, and plant dry weight were also measured. The lowest root-temperature regime (8°C) resulted in substantially lower nodule numbers and weights, and plant dry weights, than the higher temperature regimes. Development of nitrogenase activity was completely inhibited at 8°C. The immunoassay of nodule-strain occupancy showed markedly different strain-nodulation responses to the various root-temperature regimes. At 8°C, 63% of nodules were occupied by both strains. Dual strain occupancy decreased from 63% to 2% with increasing root-growth temperature, while the proportion of nodules containing only strain NRG-185 increased from 9% to 75%. Nodules containing only strain BALSAC remained relatively constant at 25% from 8° to 21°C, decreasing slightly at 25°C. The results suggest that root-environment temperatures during the period of nodule formation may have major differential effects on the success of competing rhizobial strains. If this is so, then selection of Rhizobium strains with enhanced low-temperature nodulation capabilities should be possible.
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  • 70
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    Biology and fertility of soils 6 (1988), S. 148-152 
    ISSN: 1432-0789
    Keywords: Selenite ; Se-effects on soil ; Mull ; Moder ; Mor ; N-Mineralization ; Enzym activity ; CO2-production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of Na2SeO3 on microbial activity of mull, moder and mor soils were investigated with perfusion experiments under controlled laboratory conditions. Treatments with Se at 50 mg/kg reduced carbon dioxide production and dehydrogenase activity in the mor and moder soils. Se at 250 (Ah) to 1000 (L) mg/kg had to be added to the mull soil in order to affect both parameters. In contrast to the effect on respiration and dehydrogenase activity, N mineralization, especially ammonification, was stimulated by the addition of Se. This is probably due to secondary effects such as dissolution of organic matter and P desorption. The relative inhibition of arylsulfatase activity was closely correlated with a decrease in soil respiration. It was conduded that the reduction of arylsulfatase activity in Se-treated soils was the result of a reduction in enzyme synthesis.
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  • 71
    ISSN: 1432-0789
    Keywords: Residue management ; Nitrogen fertilization ; Manure ; Burning ; Soil enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soil enzyme activities (acid and alkaline phosphatase, arylsulfatase, β-glucosidase, urease and amidase) were determined (0- to 20-cm depth) after 55 years of crop-residue and N-fertilization treatment in a winter wheat (Triticum aestivum L.)-fallow system on semiarid soils of the Pacific Northwest. All residues were incorporated and the treatments were: straw (N0), straw with fall burn (N0FB), straw with spring burn (N0SB), straw plus 45 kg N ha−1 (N45), straw plus 90 kg N ha−1 (N90), straw burned in spring plus 45 kg N ha−1 (N45SB), straw burned in spring plus 90 kg N ha−1 (N90SB), straw plus 2.24 T ha−1 pea-vine residue and straw plus 22.4 T ha−1 of straw-manure. Enzyme activities were significantly (P〈0.001) affected by residue management. The highest activities were observed in the manure treated soil, ranging from 36% (acid phosphatase) to 190% increase in activity over the control (N0). The lowest activities occurred in the N0FB (acid phosphatase, arylsulfatase and β-glucosidase) and N90 treated soils (alkaline phosphatase, amidase and urease). Straw-burning had a significant effect only on acid phosphatase activity, which decreased in spring burn treated soil when inorganic N was applied. Urease and amidase activity decreased with long-term addition of inorganic N whereas the pea vine and the manure additions increased urease and amidase activity. There was a highly significant effect from the residue treatments on soil pH. Arylsulfatase, urease, amidase and alkaline phosphatase activities were positively correlated and acid phosphatase activity was negatively correlated with soil pH. Enzyme activities were strongly correlated with soil organic C and total N content. Except for acid phosphatase, there was no significant relationship between enzyme activity and grain yield.
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  • 72
    ISSN: 1432-0789
    Keywords: A. chroococcum ; Seed inoculation ; Zea mays ; Acetylene reduction assay ; Yield response ; Associative N2 fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inoculated and non-inoculated seedlings of Zea mays were grown in agricultural soils under aseptic and non-sterile conditions. Acetylene reduction activity and microbial counts were determined after 7 and 30 days of growth. Irrespective of the soil type Azotobacter spp. were commonly isolated under maize cultivation. Inoculation of agricultural soils with a suspension of A. chroococcum led to an increase in Azotobacter numbers, although this effect diminished with time. Nitrogenase activity was detected on maize roots and increased in response to the inoculation with A. chroococcum, showing that this associative growth could be of primary importance for the plant. The results of assays for acetylene reduction activity indicated that the nitrogenase activity was associated only with the root systems.
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  • 73
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    Biology and fertility of soils 6 (1988), S. 174-177 
    ISSN: 1432-0789
    Keywords: N2 fixation ; Bradyrhizobium japonicum strains ; Soybean cultivars ; Seed inoculation ; Strain-specific fluorescent antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A field experiment was condutced in a clay loam soil to study the performance of three Bradyrhizobium japonicum strains; USDA 110, USDA 138 and TAL 379, in relation to their N2-fixing potential and competitiveness on two soybean cultivars (Clark and Calland). Inoculation of soybean cultivars with these strains, either singly or in combination, induced significant increases in plant dry weight, N2 fixation and seed yields. However, no significant differences were found between the rhizobial strains and/or their mixtures in N2 fixation and increased seed yield for both cultivars. The two soybean cultivars gave similar responses to inoculation. No significant differences in seed yield were observed between Clark and Calland cultivars. The interaction between inoculant strain and soybean cultivar was not significant. The competition between strains for nodulation was assessed. Strain USDA 110 was the most competitive, followed by USDA 138. Strain TAL 379 was always less competitive on both cultivars. The incidence of double-strain occupancy of nodules varied from 8% to 40%.
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  • 74
    ISSN: 1432-0789
    Keywords: Denitrification ; Cellulose hydrolysis ; Straw ; Cellulolytic-denitrifying bacteria ; N immobilization ; Methane ; Decreasing aerobiosis ; Permanent anaerobiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Laboratory experiments were used to examine the influence of cellulose and straw on denitrification and N immobilization in a sandy loam soil. The soil was mixed with 300 μg nitrate-N/g and incubated in a special vessel under conditions that changed from aerobic to anaerobic or in the permanent absence of O2. Gases (O2, CO2, N2, N2O, NO and CH4) were analysed by gas chromatography at regular intervals and the soil was examined for nitrate, nitrite, ammonium and cellulose. Compared with controls, the application of straw and cellulose (0.5% and 1.0%, respectively) enhanced nitrate immobilization and decreased denitrification, under both anaerobic and originally aerobic (PO2 = 20 vol%) conditions. However, a comparison of results from the aerobic and the anaerobic incubations shows that an increase in denitrification and N immobilization was apparent at an original O2 concentration of 20 vol%. N2 was the major product of denitrification in all experiments. Free methane was apparent as soon as nitrate was respired. The stimulating effect of O2 on total denitrification in the presence of relatively high amounts of easily decomposable cellulose is ascribed to a higher turnover and an intensified mineralization rate (CO2 production), which increased the total demand for electron acceptors.
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  • 75
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    Biology and fertility of soils 5 (1988), S. 328-332 
    ISSN: 1432-0789
    Keywords: Earthworm ; Aporrectodea rosea ; Radial Pressure ; Tunnelling ; Indirect tension test ; Maximum force
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The radial pressures generated by the earthworm Aporrectodea rosea were measured in a study of the conditions under which the worms were able to crack soil discs into which they were encouraged to tunnel. The tensile strength of replicate discs was determined by indirect tension tests and by expansion of rubber membranes in soil discs. Simple engineering theory was used to relate the stress required to fracture a disc with the applied internal stress. Mean values for the radial stress applied by the earthworms in tunnelling through the discs was 230 kPa. With some assumptions about the distribution of muscles across the worm diameter, it was estimated that the mean maximum force generated by all the longitudinal muscles in any segment was 3.2 N.
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  • 76
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    Biology and fertility of soils 5 (1988), S. 338-343 
    ISSN: 1432-0789
    Keywords: Blue-green algae (BGA) ; Phosphorus fertilization ; Inoculum production ; Nitrogen yield ; Cyanobacteria ; Aulosira spp. ; Aphanothece spp. ; Gloeotrichia spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field experiments were conducted to assess the effects of the application of P on growth and N yield of inoculated and indigenous blue-green algae (BGA). Addition of 17.4 kg P ha−1 in split applications led to the highest BGA biomass and N yield, 162 kg dry weight ha−1 and 6 kg N ha−1 per 15 days, respectively. When inoculum of Aulosira spp., Aphanothece spp., Gloeotrichia spp. were compared separately, Gloeotrichia spp. grew faster but Aulosira spp. fixed more N. The growth rate and N yield of Aulosira spp. decreased with high P applications, although growth continued until the application of 34.8 kg P ha−1. The effects of P on inoculum production by local species compared with those collected from other states showed the superiority of the local culture. Applications of P also enhanced the growth and N yield of indigenous BGA, with Wollea spp. showing the best results.
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  • 77
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    Biology and fertility of soils 6 (1988), S. 1-8 
    ISSN: 1432-0789
    Keywords: Barley ; Grass ; Lucerne ; Soil nematodes ; Biomass ; Respiration ; Feeding groups ; Nutrient cycling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amounts of C and N that pass through the nematode biomass in four cropping systems, barley without and with N fertilization, grass ley and lucerne, has been estimated. The nematodes were sampled at the field site of a Swedish integrated research project “Ecology of Arable Land: The Role of Organisms in Nitrogen Cycling”. The nematode biomass was lower (200 mg dry weight m−2) in the annual (barley) than in the perennial (grass and lucerne, 350 mg dry weight m−2) crops. For respiration, the nematodes used 4–71 O2m−2 year−1 corresponding to C liberation of 1.3%–2.0% of the carbon input to the soil. A higher relative contribution by bacterial-feeding nematodes to the C and N fluxes and a higher turnover rate of the nematode biomass is an indication of more rapid nutrient circulation in the annual than in the perennial cropping systems.
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  • 78
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    Biology and fertility of soils 6 (1988), S. 45-49 
    ISSN: 1432-0789
    Keywords: Soil nitrogen fractions ; Submergence ; Rice yield ; Rice nutrition ; N-uptake ; Hydrolysable ammonium N ; Amino-acid N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse experiment was conducted to study the changes in different organic fractions of soil N after application of N fertilizer as organic N alone or in combination with inorganic N. The decrease in the amount of hydrolysable NH4 + and amino-acid N was positively related to mineral-N production, grain yield and N uptake, but changes in the amount of amino sugars were not related to these three parameters. The non-hydrolysable N fraction was negatively related to grain yield and N uptake. The average mineral-N production during incubation was highly correlated with both grain yield and N uptake.
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  • 79
    ISSN: 1432-0789
    Keywords: Herbicides ; DCMU ; Diuron ; Metamitron ; Metribuzin ; Nostoc muscorum ; Cyanobacterium strain G4 ; Nitrogenase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of the photosynthesis-inhibiting herbicides Diuron, DCMU, metamitron, and metribuzin on growth and nitrogenase activity of Nostoc muscorum and a new cyanobacterial isolate, strain G4, was studied. The experiments were performed under N2-fixing photoautotrophic conditions. Both cyanobacteria showed a high degree of tolerance towards the herbicides tested. In the presence of metamitron (35, 70, and 140 ppm) and metribuzin (7, 14, 35, and 70 ppm) strain G4 proliferated as well as the control culture. Metamitron and metribuzin had no influence on the nitrogenase synthesis of strain G4 and N. muscorum. When treated with 1 μM DCMU, strain G4 and N. muscorum showed partial inhibition for the first few days, but entirely recovered during succeeding incubation. In the presence of 10 ppm Diuron, N. muscorum and strain G4, compared to the control, showed 30% and 80% nitrogenase activity, respectively.
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  • 80
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    Biology and fertility of soils 6 (1988), S. 39-44 
    ISSN: 1432-0789
    Keywords: Alnus ; Energy forestry ; Frankia ; Meadow soil ; Nitrogen fixation ; Nodulation ; Peat soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Use of the N2-fixing grey alder, Alnus incana (L.) Moench, as a short-rotation crop for energy production is currently being explored. To evaluate the need for inoculation of alders, the distribution of infective propagules of Frankia in the soil at potential sites for alder plantations was examined. Uninoculated grey alder seedlings were grown in three types of soil. Frequent nodulation was found in a meadow soil which had been free from actinorhizal plants for nearly 60 years, but the alder seedlings failed to nodulate in peat soil from two different bog sites. One of these bogs had been exploited for peat and the surface layer of the peat had been removed, so that the soil samples were taken from deep layers of the peat. At the other site, an area of cultivated peat, there were no infective propagules of Frankia in plots without alders; the infective Frankia was present in plots only where it had been introduced by inoculated alders. There was no detectable air-borne dispersal of Frankia. Instead, water movement might account for the dispersal of Frankia in peat. Although the apparent absence of Frankia in these peat soils necessitates inoculation of alder seedlings before planting out, this makes it possible to introduce and maintain Frankia strains with selected beneficial characteristics, since there is no competition from an indigenous Frankia flora.
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  • 81
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    Biology and fertility of soils 6 (1988), S. 61-64 
    ISSN: 1432-0789
    Keywords: Ammonia emission ; Ammonia absorption ; Nitrogen cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Knowledge of the contribution of NH3 to the total deposition of N in rural areas is sparse, because the determination of NH3 deposition is costly and labour-intensive. A simple biomonitor consisting of barley plants grown in pots with an inert growth medium is therefore proposed for estimating total N deposition, including NH3. The rise in total N of the plant-soil system reflects the deposition. The biomonitor was tested near a dairy farm. Different N contents in the green biomass reflected differences in deposition, and the deposition correlated very well with NH3 levels in the area and in two background stations. The biomonitors were placed above the crop, the measurements thus representing total N at the edge of a plant community. In 1 month the deposition in the NH3 plume was 8 kg N ha−1.
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  • 82
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    Biology and fertility of soils 6 (1988), S. 84-88 
    ISSN: 1432-0789
    Keywords: Rhizobium ; Pigeonpea ; Cajanus cajan ; Effective legume symbiosis ; Antibiotic resistant rhizobia ; Cowpea rhizobia ; Acetylene reduction activity (ARA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pot and laboratory experiments were conducted to study the establishment and effectiveness of a streptomycin-sulphate-resistant (1 mg/ml of medium) pigeonpea rhizobia strain (RM7) in sterile sand and non-sterile soils. Strain RM7 increased the drymatter yield of pigeonpea plants (Cajanus cajan) by 106% over control plants under sterile conditions. However, when the rhizobia strain was introduced into 14 different non-sterile soils with a narrow abiotic variability, the comparable beneficial effect was observed only in one soil inoculated with log 6.70 cells/pot. At this inoculum rate, the percentage increase in yield over control plants varied from −1 to 140 in different soils. Rhizobium (RM7), applied at log 3.70 cells/pot (3 kg soil), showed less than 5% establishment in four soils. However, establishment varied from 8% to 72% at a higher level of inoculation (log 6.70 cells/pot). Displacement of native rhizobia and creation of new sites for nodulation by the introduced rhizobia were also affected by soil properties. The increase in shoot dry-matter yield compared with control plants was positively correlated with the percent establishment of RM7 (r = 0.60*) in these soils. Experiments showed that some biotic stresses led to poor survival, proliferation and establishment of the added alien in the soil. Therefore, any culture that is efficient in one soil may not produce similar results under all situations.
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  • 83
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    Biology and fertility of soils 6 (1988), S. 100-105 
    ISSN: 1432-0789
    Keywords: Decomposition ; Herbicides ; Microbial inhibitors ; Cotton ; Herbicide effects ; Trifluralin ; 14CO2 evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of trifluralin and 12 of its soil-formed metabolites on the decomposition of radio-labelled glucose, protein and cellulose were determined, using 14CO2 evolution from soil as a measure of decomposition. Trifluralin increased 14C-glucose mineralization rates, but these increases could be eliminated by adding N. Trifluralin had no inhibitory effect on the mineralization of protein or cellulose, but five of the metabolites inhibited glucose mineralization. None of the trifluralin metabolites affected protein mineralization. Seven trifluralin metabolites increased the rate of cellulose mineralization when applied at rates exceeding those that would be expected in soil. After considering the rate of metabolite application and the magnitude of the responses observed these compounds are expected to have no major effects on the microbial decomposition processes in soil.
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  • 84
    ISSN: 1432-0789
    Keywords: Soil mycelia ; Direct observation ; Membrane filtration ; Agar film ; Extraction efficiency ; Staining procedures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A rapid and simple membrane filtration technique to prepare specimens for light microscopy — direct observation of soil mycelial populations —has been developed from past studies. Stain type, and soil extraction and dilution procedures were optimized. The technique involves homogenizing soil suspensions for 60s followed by serial dilution and filtration of suspended soil through a membrane filter to a maximum of 1 mg soil per cm2 effective filtering area. Compared with the fluorescein isothiocyanate or acridine orange fluorescent stains, fluorescent brightener stained far greater amounts of mycelia from three fungal species grown in vitro for 2 and 46 days (25°C), and also indigenous mycelia in two grassland soils. Use.of the membrane filtration technique with fluorescent brightener stain also produced significantly (P ≤ 0.05) greater estimates of soil mycelial lengths than the agar film preparative technique with phenolic aniline blue stain and phase-contrast microscopy. This was attributed to the staining efficiency of fluorescent brightener, as both the membrane filtration and the agar film techniques produced similar mycelial length and volume estimates g−1 soil when fluorescent brightener stain was used for both.
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  • 85
    ISSN: 1432-0789
    Keywords: N2-fixation ; 15N ; Cicer arietinum ; Isotope-dilution ; Acetylene reduction assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary N accumulation, nodulation, and acetylene reduction activity were measured at frequent intervals during the growth of two chickpea genotypes, and N2 fixation was estimated by an isotope-dilution method, using safflower as a non-N2-fixing reference. Safflower was more efficient at N uptake than both the chickpea genotypes for at least the first 50 days and thus could not be used as an accurate reference control. We recommend that further work should employ non-nodulatiog genotypes of chickpea as reference plants and use slow-release forms of 15N fertilizer. Direct genotype comparison by isotope dilution estimated that genotype K 850 fixed 16–18 kg ha−1 more N than G 130, and this difference was supported by the greater nodule mass and acetylene reduction activity in the K 850 cultivar. Inoculation with an ineffective chickpea Rhizobium sp. led to 69% nodulation on cultivar K 850 but only 33% on G 130. While nodule weight, N uptake, and acetylene reduction activity decreased with inoculation in K 850, the isotope dilutions were similar for both inoculation treatments. The lack of a significant effect on N2 fixation was ascribed to the partial success of inoculant establishment.
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  • 86
    ISSN: 1432-0789
    Keywords: A. chroococcum ; Zea mays ; Yield response ; Fertilizer N ; Inoculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field experiments were conducted during 1985 and 1986 to examine the effect of Azotobacter chroococcum on the grain yield of maize. Application of 40 kg N ha−1 plus A. chroococcum caused a significant increase in maize yield. Azotobacter inoculation was more efficient at lower doses (40 kg N ha−1) than at high doses (80 kg N ha−1) of urea.
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  • 87
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    Biology and fertility of soils 6 (1988), S. 354-355 
    ISSN: 1432-0789
    Keywords: Symbiotic effectiveness ; Rhizobium spp. ; Chickpea ; Cicer arietinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Antibiotic-resistant Rhizobium spp. strains have been used in ecological studies of legumerhizobia symbiosis. It has been suggested that in the course of acquiring resistance against high doses of antibiotics, rhizobia might lose their symbiotic effectiveness. Evidence both for and against this argument has been presented (Kremer and Peterson 1982; Materon and Hagedron 1983). This communication reports our experience with streptomycin-resistant (Str+) mutants of chickpea Rhizobium spp. strains. Parent strains were used as controls.
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  • 88
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    Biology and fertility of soils 7 (1988), S. 1-6 
    ISSN: 1432-0789
    Keywords: Denitrification ; Air-filled porosity ; Rhizosphere effect ; Organic carbon content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of the air-filled porosity and organic-matter content of the soil on denitrification with non-limiting NO3 − concentrations was studied in unplanted pots and in pots sown to wheat. Four organic-C levels were established by using pure and mixed soil material from a Bt horizon with 0.12% organic-C and an Ap horizon with 1.31% organic C from a mollic luvisol. A range of air-filled porosities from 3% to 25% during denitrification assays was obtained by varying soil compaction. Beyond a 10% to 12% threshold of air-filled porosity the denitrification rates were at an insignificant and constant level in planted as well as in unplanted soil for all organic-C contents. Below this threshold denitrification increased exponentially with decreasing air-filled porosity. In planted soil the excess of denitrification over that of unplanted soil was inversely related to air-filled porosity. This rhizosphere effect on denitrification, which was confined to air-filled porosities lower than 10%–12%, became significantly greater with increasing soil organic-C content. The findings indicate that root dependent respiration amplifies O2 depletion in the rhizosphere and may accelerate the onset of denitrification in planted soil.
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  • 89
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    Biology and fertility of soils 7 (1988), S. 23-27 
    ISSN: 1432-0789
    Keywords: Eudrilus eugeniae ; Earthworm ; Cocoons ; Incubation ; Reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Although various authors have contributed to our knowledge of the biology of Eudrilus eugeniae some basic facts about the life cycle and reproduction are still not fully documented. Quantitative observations were made of the cocoons at 25 °C in different substrates. The incubation period for 166 cocoons was 16.89 days, based on cocoons produced by worms between the ages of 70 and 100 days. These cocoons produced a mean 2.12 hatchlings per cocoon after incubation in cattle manure, moist filter paper and distilled water. A smaller batch of cocoons incubated in cattle manure produced a mean of 2.7 hatchlings per cocoon. The hatching success of the cocoons was 84% in cattle manure, 50% in distilled water, and 48% on moist filter paper. The reproductive capabilities of E. eugeniae and Eisenia fetida were compared.
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  • 90
    ISSN: 1432-0789
    Keywords: 15N ; N2 fixation ; Rhizosphere ; Sorghum bicolor ; Pennisetum americanum ; Acetylene reduction assay (ARA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a series of short-term experiments root systems of young sorghum and millet plants inoculated with N2-fixing bacteria were exposed to 15N2-enriched atmospheres for 72 h. The plants were grown in a normal atmosphere for up to 22 days after the end of the exposure to allow them to take up the fixed N2. Environmental conditions and genotypes of sorghum and millet were selected to maximise N2-fixation in the rhizosphere. Detectable amounts of fixed N (〉 16 μg/plant) were rapidly incorporated into sorghum plants grown in a sand/farmyard manure medium, but measurable fixation was found on only one occasion in plants grown in soil. N2 fixation was detectable in some experiments with soil-grown millet plants but the amounts were small (2–4 μg/plant) and represented less than 1 % of plant N accumulated over the same period. In many cases there was no detectable 15N2 incorporation despite measurable increases in ethylene concentration found during an acetylene reduction assay.
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  • 91
    ISSN: 1432-0789
    Keywords: Ipomoea batatas ; Vesicular-arbuscular mycorrhiza ; Phosphorus uptake ; Single superphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Mycorrhizal infection in the roots of 10 sweet potato cultivars was assessed 7 weeks after planting in three soils collected from Ibadan, Fashola and Onne in southern Nigeria, three soils which contained 21.0, 7.8 and 54.8 mg P kg−1, respectively. Mycorrhizal infection averaged 17% in the soil from Ibadan, 24% in the soil from Fashola and 7% in the acid soil from Onne. The plants grown in the Fashola soil contained the same percentage of P as plants grown in the Onne soil. Although the percentage of P in sweet potato was lowest in the Ibadan soil, shoot dry weights were 35% higher in this soil than in the other two soils. There was no correlation between the level of mycorrhizal infection and plant dry weight in the partially sterilized soil from Ibadan. Sweet potato inoculated in this soil with infected roots of Leucaena leucocephala showed a higher level of mycorrhizal infection than uninoculated plants. Dry-matter production was, however, the same for all treatments. The sweet potato cultivars differed in their level of mycorrhizal infection and in their response to applied P. Cultivars TIS 2498 and TIS 70357 consistently showed the lowest percentage of infection; and TIb 4, TIS 8441 and TIS 8524 showed infection levels above 20% in the Fashola and Ibadan soils. When the low-yielding cultivar, TIb 4, and an improved clone, TIS 9265, were grown in the presence of 50 and 100 mg single superphosphate per kg soil, TIb 4 produced more dry matter in the presence of P fertilizer than it did without the fertilizer. Growth and mycorrhizal infection of TIS 9265 were not affected by the fertilizer.
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  • 92
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    Biology and fertility of soils 7 (1988), S. 16-22 
    ISSN: 1432-0789
    Keywords: Nitrification activity ; Submerged soils ; Denitrification ; 15N balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nitrification activity (formation of NO 2 − + NO 3 − per unit soil weight) was measured in the surface layer of 15 presubmerged soils incubated in petri dishes under flooded but aerobic conditions. soils with pH above 5 nitrified quickly, whereas soils with pH below this level did not nitrify or nitrified slowly. The pH values between 7 and 8.5 were optimal for nitrification. Organic-matter levels in the 15 soils of our study did not influence their nitrification activities. In a follow-up greenhouse pot study, after a period of 3 weeks, 15N-balance measurements showed that the loss of N through apparent denitrification did not follow the nitrification patterns of the soils observed in the petri dishes. Apparent denitrification accounted for 16.8% and 18.9% loss of 15N from a soil with insignificant nitrification activity and a soil with high nitrification activity, respectively. These results, thus, indicate a lack of correspondence between the nitrification activities of soil and the denitrification loss of N when the former was measured in the dark and the latter was estimated in the light. Soils that nitrified in the darkness of the incubator did not nitrify in the daylight in the greenhouse.
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  • 93
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    Biology and fertility of soils 7 (1988), S. 46-52 
    ISSN: 1432-0789
    Keywords: Nematodes ; Microarthropods ; Agroecosystems ; N mineralization ; Sorghum bicolor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Intact cores of agricultural soil planted with Sorghum bicolor were treated with selective biocides or combinations of biocides to manipulate soil organisms. Half the replicates of each biocide treatment were also given N fertilizer. The plants were maintained in a greenhouse, where growth and nutrient content and soil-organism populations were monitored over 16 weeks. The plants responded strongly to fertilization, but showed weak and variable responses to the biocides, even though biocide treatments aimed at animal taxa effectively reduced their target groups. There were no strong interactions between faunal manipulations and fertilization, implying that there was little compensatory function of fauna in the absence of fertilizer. Conditions under which soil fauna are important in making mineral nutrients available to plants in the field need further investigation.
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  • 94
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    Biology and fertility of soils 7 (1988), S. 39-45 
    ISSN: 1432-0789
    Keywords: Earthworm species ; Surface casting ; Allolobophora chlorotica ; Aporrectodea caliginosa ; Aporrectodea longa ; Lumbricus spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Surface cast production was investigated for several species of earthworms, including some not normally considered to produce surface casts. In single-species culture, the amount of cast material deposited on the surface by different species varied, with Allolobophora chlorotica 〈 Lumbricus rubellus 〈 Aporrectodea caliginosa 〈 Aporrectodea longa. In field communities, results indicated that a single species dominated surface casting activity. The order of species dominance was consistent with the above findings from single-species cultures, if Lumbricus terrestris was considered to be equivalent to Ap. longa. Surface casting is an essential function within earthworm communities which maintains their living space. However, it involves an energy cost and carries a risk of predation. Therefore, there are disadvantages associated with this activity. All of the species studied produced surface casts unless other, usually larger, species were present.
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  • 95
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhiza ; N2 fixation ; 15N-labelled fertilizers ; Rhizobium ; Legume symbiosis ; Drought stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The legume Medicago sativa (+Rhizobium melilott) was grown under controlled conditions to study the interactions between soluble P in soil (four levels), or a mycorrhizal inoculum, and the degree of water potential (four levels) in relation to plant development and N2 fixation. 15N-labelled ammonium sulphate was added to each pot for a qualitative estimate of N2 fixation, in order to rank the effects of the different treatments. Dry-matter yield, nutrient content and nodulation increased with the amount of plant-available P in the soil, and decreased as the water stress increased, for each P-level. The mycorrhizal effect on dry matter, N yield, and on nodulation was little affected by the water potential. Since P uptake was affected by the water content in mycorrhizal plants, additional mechanisms, other than those mediated by P, must be involved in the mycorrhizal activity. There was a positive correlation between N yield and nodulation for the different P levels and the mycorrhizal treatment at all water levels. A high correlation between plant unlabelled N content and atom% 15N excess was also found for all levels of P. In mycorrhizal plants, however, the correlation between unlabelled N yield and 15N was lower. This suggests that mycorrhiza supply plants with other N sources in addition to those derived from the improvement on N2 fixation.
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  • 96
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    Biology and fertility of soils 7 (1988), S. 32-38 
    ISSN: 1432-0789
    Keywords: Hydrolysable N ; Mineralizable N ; N2-fixation ; Priming effect ; Plant available N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Non-symbiotic N2 fixation was studied under laboratory conditions in two soils from Pakistan (Hafizabad silt loam and Khurrarianwala silt loam) and one from Illinois, USA (Drummer silty clay loam) incubated in a 15N-enriched atmosphere. N2 fixation was greatest with the Drummer soil (18–122 μg g−1 soil, depending upon the soil treatment) and lowest with the Khurrarianwala soil (4–81 μg g−1 soil). Fixation was increased by the addition of glucose, a close correlation being observed between the amount of glucose added and the amount of N2 fixed in the three soils (r = 0.96). Efficiency of N2 fixation varied with soil type and treatment and was greatest in the presence of added inorganic P. Application of Mo apparently had a negative effect on the amount and efficiency of N2 fixation in all the soils. The percentage of non-symbiotically fixed 15N in potentially mineralizable form (NH 4 + -N released in soil after a 15-day incubation period under anaerobic conditions) was low (2%–18%, depending upon the soil treatment), although most of the fixed N (up to 90%) was recovered as forms hydrolysable with 6N HCl. Recovery in hydrolysable forms was much greater for the fixed N than for the native soil N, indicating that the former was more available for uptake by plants.
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  • 97
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    Biology and fertility of soils 5 (1988), S. 282-287 
    ISSN: 1432-0789
    Keywords: Soil fauna ; Decomposition ; CO2 production ; Birch litter ; Raw humus ; Microcosm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of diverse soil fauna (Collembola, Acari, Enchytraeidae, Nematoda) on decomposition of dead organic matter was studied in microcosms containing (1) birch leaf litter, (2) raw humus of coniferous forest and (3) litter on humus. Total respiration (CO2 evolution) was monitored weekly, and mass loss, length of fungal hyphae (total and metabolically active) and survival of animal populations were checked at the end of weeks 12 and 21–22 from the start of experiment. Animal populations established themselves well during the incubation. At the end of the experiment some replicates containing litter had microarthropod densities of up to 500 specimens per microcosm, corresponding to a field population of 200 000 m−2. The soil animals had a positive influence on total respiration in all substrates. By the end of experiment 32.0%, 22.6% and 14.6% more CO2 had evolved in the presence of animals in litter, litter + humus and humus alone, respectively. There was clear trend towards a higher mass loss in the presence of animals, though it was significant in litter only. Our results showed that a diverse soil animal community enhances the activity of soil microbes, and may thereby accelerate decomposition in raw coniferous forest soil.
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  • 98
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    Biology and fertility of soils 5 (1988), S. 299-303 
    ISSN: 1432-0789
    Keywords: Nonyphenol ; Persistence ; Biotoxicity ; CO2 production ; ATP-biomass ; Compost ; Soil systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The persistence and biotoxicity of nonylphenol, a mixture of monoalkyl phenols that is found in relatively high concentrations in sewage sludge, were studied in an incubation experiment with a reconstituted soil system (compost + sandstone). The effect of nonylphenol (100 and 1000 ppm) on CO2 evolution and biomass ATP were monitored. Nonylphenol depressed CO2 production significantly only at high concentrations [1000 ppm 4-nonylphenol (4-NP)]. Biomass ATP declined progressively after the 5th day. At 100 ppm no toxic effects were detected. After a lag phase, nonylphenol disappeared readily upon incubation at the lower concentration (100 ppm), but persisted at high levels (1000 ppm). The persistence of 4-nonylphenol increased under aseptic conditions. The chromatogram profile of 4-NP changed over time, indicating that some of the isomers are more easily degraded than others.
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  • 99
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    Biology and fertility of soils 5 (1988), S. 313-316 
    ISSN: 1432-0789
    Keywords: Nematicide ; Phosphorus ; Subleaflet P ; Tropeptic Eutrustox ; Leucaena leucocephala ; VA mycorrhiza ; Glomus aggregatum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Concentrations of phenamiphos ranging from 0 to 40 μrg/g soil were established in a typical Oxisol (Tropeptic Eutrustox), inoculated or uninoculated with Glomus aggregatum. The effect of the nematicide on the development of vesicular-arbuscular mycorrhizal (VAM) symbiosis was evaluated in the greenhouse using Leucaena leucocephala as an indicator host plant. Treatment of soil with phenamiphos did not have a significant influence on the development of mycorrhizal activity measured in terms of subleaflet phosphorus concentrations. Similarly, the nematicide did not have an adverse effect on the level of mycorrhizal colonization or on the P content of shoots, as determined at the time of harvest. However, shoot dry weight was adversely influenced by phenamiphos when the chemical was applied to the uninoculated soil at 20 μg/g soil or higher, and when it was applied to the inoculated soil at 40 μg/g soil. It is concluded that phenamiphos is not likely to influence the growth of Leucaena or its symbiotic association with VAM fungi if the concentrations applied do not exceed levels known to suppress nematodes.
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  • 100
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    Biology and fertility of soils 6 (1988), S. 26-32 
    ISSN: 1432-0789
    Keywords: Organic S ; S uptake ; Plants ; Greenhouse experiments ; Leaching tubes ; Rate constants ; Sulfur mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field-moist soil and glass beads mixtures were packed in glass tubes and leached with 100 ml of 5 mM CaCl2 and incubated at 20 or 30°C. The leaching procedure was repeated every 2 weeks for 14 weeks. The leachates were analysed for SO inf4 sup2− and NO3 −. The S uptake by three successive croppings of corn (Zea mays L.) or soybean [Glycine max (L.) Merr.] at 40- or 60-day intervals, respectively, or three cuttings of ryegrass (Lolium multiflorum L.) at 30-day intervals were studied under greenhouse conditions. Results showed that significantly greater amounts of S were mineralized at 30°C than at 20°C in each of 13 Iowa and 7 Chilean surface soils. Expressed as percentages of organic S in soils, the amounts of S mineralized in the Iowa surface soils in 14 weeks at 20 and 30°C ranged from 1.2% to 9.8% and from 2.4% to 17.5%, respectively. The corresponding values for the Chilean soils ranged from 0.9% to 7.2%6 and from 1.4% to 12.1%. The Q10 values of S mineralization ranged from 1.7 to 4.4 (average 2.5) for the Iowa soils and from 1.7 to 3.1 (average 2.1) for the Chilean soils. The cumulative S mineralized at 20°C in 14 weeks was significantly correlated with the cumulative N mineralized (linear model, r=0.72**; quadratic model, r=0.84***). Similarly, the cumulative S mineralized at 30°C was significantly correlated with the cumulative N mineralized at this temperature (linear model, r=0.81***; quadratic model, r = 0.82***). The potentially mineralizable S pool (S0), calculated by using an exponential equation for the S mineralized at 20°C, ranged from 5 to 44 mg kg−1 for the Iowa soils and from 10 to 25 mg kg−1 for the Chilean soils. The corresponding values obtained by using a reciprocal-plot technique ranged from 6 to 48 mg kg−1 and from 12 to 26 mg kg−1, respectively. The S0 values calculated for S mineralized at 30°C, in general, were higher than those obtained at 20°C. The S mineralization rate constant (k) and the time required to mineralize 50% of S0 (K t), calculated by using the cumulative SO inf4 sup2− released during 14 weeks of incubation, varied considerably among the soils. Up take of S by corn and soybean (tops+roots) were, in general, lower than the total SO inf4 sup2− mineralized in 14 weeks at 20°C.
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