Summary
Nitrification activity (formation of NO −2 + NO −3 per unit soil weight) was measured in the surface layer of 15 presubmerged soils incubated in petri dishes under flooded but aerobic conditions. soils with pH above 5 nitrified quickly, whereas soils with pH below this level did not nitrify or nitrified slowly. The pH values between 7 and 8.5 were optimal for nitrification. Organic-matter levels in the 15 soils of our study did not influence their nitrification activities. In a follow-up greenhouse pot study, after a period of 3 weeks, 15N-balance measurements showed that the loss of N through apparent denitrification did not follow the nitrification patterns of the soils observed in the petri dishes. Apparent denitrification accounted for 16.8% and 18.9% loss of 15N from a soil with insignificant nitrification activity and a soil with high nitrification activity, respectively. These results, thus, indicate a lack of correspondence between the nitrification activities of soil and the denitrification loss of N when the former was measured in the dark and the latter was estimated in the light. Soils that nitrified in the darkness of the incubator did not nitrify in the daylight in the greenhouse.
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References
Bock E (1965) A comparative examination of the effect of visible light on Nitrosomonas europaea and Nitrobactor winogradskyi. Arch Mikrobiol 51:18–41
Bouldin DR, Alimagno BV (1976) NH3 volatilization from IRRI paddies following broadcast application of fertilizer nitrogen. Int Rice Res Inst (Los Baños)
Buresh RJ, Austin ER (1986) Measurement of denitrification in flooded rice fields. Agronomy Abstracts
Buresh RJ, Austin ER, Craswell ET (1982) Analytical methods in 15N research. Fert Res 3:37–62
Dancer WS, Paterson LA, Chester G (1973) Ammonification and nitrification of N as influenced by soil pH and previous N treatments. Soil Sci Soc Am Proc 37:67–69
Fillery IRP, Vlek PLG (1986) Reappraisal of the significance of ammonia volatilization as an N loss mechanism in flooded rice fields. Fert Res 9:79–98
Fillery IRP, Simpson JR, De Datta SK (1985) Contribution of ammonia volatilization to total nitrogen loss after application of urea to wetland rice fields. Fert Res 8:193–202
Focht DD (1979) Microbial kinetics of nitrogen losses in flooded soils. In: Nitrogen and rice. Int Rice Res Inst (Los Baños), pp 119–134
Hauck RD (1979) Methods for studying N transformation in paddy soils: Review and comments. In: Nitrogen and rice. Int Rice Res Inst (Los Baños), pp 73–94
Howeler RH, Bouldin DR (1971) The diffusion and consumption of oxygen in submerged soils. Soil Sci Soc Am Proc 35:202–208
Kamphake LJ, Hannah SA, Cohen JM (1967) Automated analysis for nitrate by hydrazine reduction. Water Res 1:205–216
Khaldebarin B, Oertli JJ (1977) Effect of pH and ammonia on the rate of nitrification of surface water. J Water Pollut Control Fed 49:1688–1692
Mitsui S (1977) Recognition of the importance of denitrification and its impact on various improved and mechanized application of nitrogen to rice plant. In: Proc Int Seminar on Soil Environment and Fertility Management in Intensive Agriculture (SEFMIA). Tokyo, Japan, pp 259–268
Morrill LG, Dawson JE (1967) Patterns observed for the oxidation of ammonium to nitrate by soil organisms. Soil Sci Soc Am Proc 31:757–760
Reddy KR, Patrick WH Jr (1976) Effect of frequent changes in aerobic and anaerobic conditions in redox potential and nitrogen loss in a flooded soil. Soil Biol Biochem 8:491–495
Reddy KR, Patrick WH Jr (1986) Fate of fertilizer nitrogen in the rice root zone. Soil Sci Soc Am J 50:649–651
Sahrawat KL (1982) Nitrification in some tropical soils. Plant Soil 65:281–286
Sakai H (1970) A method to determine nitrifying power of paddy soil and some results with its use. Progress report of the all India coordinated rice improvement project, vol 2. Kharif, Rajendra Nagar, Hyderabad, Andhra Pradesh, India
Sarathchandra SU (1978) Nitrificaton activities and the changes in the populations of nitrifying bacteria in soil perfused at two different H-ion concentrations. Plant Soil 50:99–111
Savant NK, De Datta SK (1982) Nitrogen transformations in wetland rice soils. Adv Agron 35:241–302
Schmidt EL (1982) Nitrification in soil. In: Stevenson FJ (ed) nitrogen in agricultural soils. Agron Monogr 22, ASA-CSSA-SSSA, Madison, Wisc, pp 253–288
Simpson JR, Freney JR, Muirhead WA, Leuning R (1985) Effects of phenylphosphoromolybdate and dicyandiamide on nitrogen loss from flooded soils. Soil Sci Soc Am J 49:1426–1431
Vlek PLG, Craswell ET (1979) Effect of nitrogen source and management on ammonia volatilization losses from flooded rice soil systems. Soil Sci Soc Am J 43:352–358
Vlek PLG, Craswell ET (1981) Ammonia volatilization from flooded soils. Fert Res 2:227–245
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Katyal, J.C., Carter, M.F. & Vlek, P.L.G. Nitrification activity in submerged soils and its relation to denitrification loss. Biol Fert Soils 7, 16–22 (1988). https://doi.org/10.1007/BF00260726
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DOI: https://doi.org/10.1007/BF00260726