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  • Angiosperms  (46)
  • Nitrogen fixation  (29)
  • Electron microscopy  (27)
  • calcium  (26)
  • bioavailability
  • temperature
  • Springer  (165)
  • Periodicals Archive Online (PAO)
  • 1985-1989  (165)
  • 1980-1984
  • 1985  (165)
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  • 1985-1989  (165)
  • 1980-1984
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Colloid & polymer science 263 (1985), S. 116-119 
    ISSN: 1435-1536
    Schlagwort(e): Electron microscopy ; staining ; morphology ; nylon-12 ; orientation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Maschinenbau
    Notizen: Abstract The morphology of drawn and annealed sheets of nylon-12 was investigated by transmission electron microscopy of stained sections, and the results compared with equivalent small-angle X-ray scattering (SAXS) patterns. A three-component structure was observed, consisting of crystalline (C) and amorphous (A) regions in the microfibrils and an interfibrillar component whose density was deduced to be intermediate between that of the C and A regions. The crystallite width was given satisfactorily by a Guinier analysis of the SAXS profile.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Colloid & polymer science 263 (1985), S. 454-461 
    ISSN: 1435-1536
    Schlagwort(e): Impregnation ; void ; liquid ; fabric ; temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Maschinenbau
    Notizen: Abstract Under the condition that liquid epoxy resin impregnation was through polyester non-woven fabric sheets, sandwiched between two circular glass plates, the increase in gas pressure caused by decrease in the volume of non-impregnating area was not as much as expected according to Boyle's law. Hence, the quantity of the gas consumed by dissolution and diffusion into the liquid resin and the polyester fibers, as well as by the void formation in the impregnating area of liquid resin, was calculated. According to the results, the quantities of gas decreased by dissolution and diffusion from the non-impregnating area into the impregnating liquid resin and into the polyester fiber are found to be so small that the deviation from Boyle's law mostly depends on the gas consumption due to the void formation in the impregnating area. Observing the impregnating region with a microscope, many voids were found in the area between the non-impregnating area and the completely impregnated area. The number and size of voids are theoretically estimated by assuming the non-uniformity of the fiber distribution in the polyester non-woven fabric.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1429-1430 
    ISSN: 1420-9071
    Schlagwort(e): Fish ; serotonin ; hydrostatic pressure ; temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The contents of serotonin (5 HT) and its metabolite 5 hydroxy indoleacetic acid (5 HIAA) have been measured (HPLC technique) in the brains of eels exposed to different conditions of hydrostatic pressure and temperature (HP=1 or 101 ATA in winter, Tw=14°C, and in summer, Tw=19°C). It appears that an increase of Tw induces a significant increase of the 5 HT/5 HIAA ratio. In contrast, eels exposed at 101 ATA of HP for 1 h do not exhibit any modification in the 5 HT/5 HIAA brain ratio at a given temperature. The involvement of 5 HT under the conditions studied is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1532-1533 
    ISSN: 1420-9071
    Schlagwort(e): Marlin ; muscle ; mechanics ; ATPase activity ; temperature ; skinned fibers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary ATPase activity and force generation have been measured simultaneously in isolated, demembranated muscle fibers of the Pacific blue marlin (Makaira nigricans) between 0 and 30°C. Tension generation is relatively independent of temperature above 15°C and falls with a Q10 of 〈1.5 on decreasing the temperature to 0°C. In contrast, the Q10 for ATPase activity is 2.2 over the range 0–30°C. The results are interpreted in terms of the cross bridge theory of contraction.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 38 (1985), S. 165-169 
    ISSN: 1570-7458
    Schlagwort(e): temperature ; growth rates ; cassava mealybug ; Phenacoccus manihoti ; life table ; Manihot esculenta
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Des tables de vie ont été établies pour déterminer l'effet de températures constantes sur les taux de croissance de populations de la cochenille du manioc, Phenacoccus manihoti Mat.-Ferr., et ainsi comprendre les changements au sein des populations du ravageur dans les champs et mener à bien le programme de lutte biologique organisé à l'I.I.T.A. contre cette cochenille. P. manihoti, introduit à partir de l'Amérique latine en Afrique, y menace la production du manioc (Manihot esculenta Crantz). Le taux intrinsèque d'accroissement natural (rm) a augmenté de 0.114 à 20°C, à 0.185 à 27°C, avant de descendre à 0.182 à 30.5°C. Le taux net de reproduction (Ro) a été relativement élevé (426–584 oeufs femelles/génération). Dans nos conditions expérimentales, la mortalité a atteint 50% au bout de 37.5, 21.5, 19.0 jours respectivement à 20, 23.5, 27 et 30.5°C. La durée du cycle et le coefficient d'accroissement (λ) étaient inversement liés à la température. Le ravageur possède la capacité de doubler sa population en 6.08 jours à 20°C alors que 3.81 jours seulement suffisent pour doubler la population à 30.5°C. Ces résultats nous ont permis de comprendre et d'expliquer l'énorme pouvoir de pullulation de la cochenille observé dans les champs pendant la saison sèche; il s'ensuit que les lâchers des entomophages produits en élevages doivent se faire très tôt au début de la saison sèche, afin de contrecarrer la grande fertilité et la capacité d'augmentation rapide des populations de P. manihoti.
    Notizen: Abstract Life table studies were conducted to assess the effect of constant temperature on the rate of population growth of the cassava mealybug, Phenacoccus manihoti Matile-Ferrero. Four temperatures, between 20 and 30.5°C, were tested. An inverse relationship was observed between temperature and most demographic parameters. The intrinsic rate of natural increase (rm) increased from 0.1 at 20°C to 0.2 at 27°C and 30.5°C. The net reproductive rate varied between 426.3 at 30.5°C and 584.7 at 20°C. The mealybug population reached 50% mortality after 37.5, 21.5, 19.0 and 19.0 days respectively at 20, 23.5, 27 and 30.5°C. The results indicate that P. manihoti can persist and increase in numbers within the range between 20 and 30.5°C.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 39 (1985), S. 61-71 
    ISSN: 1570-7458
    Schlagwort(e): Dacus tryoni ; Tephritidae ; Diptera ; fruit flies ; oviposition ; egg laying ; behaviour ; taste receptors ; chemoreceptors ; stimulant ; deterrent ; fructose ; calcium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Des mouches fruitières gravides du Queensland (Dacus tryoni), confinées au laboratoire dans des chambres d'oviposition sont stimulées par la présence de β-D(-)fructose, à pondre significativement plus d'oeufs dans un substrat gélosé. Ce composé est un véritable stimulant d'oviposition, accroissant le nombre d'oeufs déposés par mouche, plutôt que simplement localisant l'oviposition dans les substrats le contenant. Le fructose est effectif seulement lorsqu'il est accessible aux récepteurs gustatifs tarsaux et labelliaux et, apparement, agit en stimulant de plus fréquentes insertions de l'ovipositeur dans le substrat; le contact du fructose avec uniquement l'ovipositeur inséré, n'accroît pas l'oviposition. Le seuil de concentration pour obtenir une stimulation par le fructose est de 4 mM; la résponse maximale se produit à 50 mM et au delà, auxquelles concentrations l'oviposition est augmentée d'un facteur 6 par rapport au témoin, qu'il y ait ou non possibilité de choix de substrat. Le sucrose (testé à 100 et 1 000 mM) et le D-glucose (testé à 100 et 500 mM) ne stimulent pas l'oviposition chez D. tryoni. Le fructose favorise fortement l'oviposition grâce aux trous existants dans une surface impénétrable, et dans les conditions naturelles, D. tryoni l'utilise probablement comme un marqueur pour localiser les ruptures dans la peau des fruits, où l'insertion est plus facile. La présence de chlorure de calcium molaire dans la gélose fructose inhibe fortement l'oviposition, même lorsqu'il est inaccessible aux récepteurs gustatifs tarsaux et labelliaux. Le chlorure de sodium molaire n'est pas inhibiteur. Les ions calciums déploient apparemment leur effet inhibiteur par l'intermédiaire de récepteurs gustatifs localisés sur l'ovipositeur.
    Notizen: Abstract Gravid Queensland fruit flies (Dacus tryoni) are stimulated by the presence of β-D(-) fructose to lay significantly more eggs in an agar substrate. Fructose is only effective when accessible to the tarsal and/or labellar gustatory sensilla; it greatly increases oviposition through holes in an impenetrable membrane. Threshold for the fructose effect is 4 mM, maximal response being at 50 mM and above. Sucrose and glucose are not oviposition stimulants for D. tryoni. In the field situation D. tryoni probably uses fructose as a marker to locate breaks in the skin of ripe fruit, where insertion of the ovipositor is easier. The flies are deterred from ovipositing in fructose agar by the presence of molar calcium chloride, even when this is inaccessible to the tarsal and labellar gustatory sensilla. Molar sodium chloride is not inhibitory. Calcium ions apparently exert their inhibitory effect via gustatory sensilla located on the ovipositor.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Methods in cell science 9 (1985), S. 83-93 
    ISSN: 1573-0603
    Schlagwort(e): keratinocytes ; human ; epidermis ; serum-free ; calcium ; differentiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Methods are described for serum-free culture of human epidermal keratinocytes derived from neonatal foreskin tissue. Cultures are initiated, stored frozen, and returned to active growth, all with bovine pituitary extract as the only undefined supplement. Clonal growth assays are then performed in a biochemically defined medium. The degree of stratification and differentiation in the defined medium (and also with pituitary extract) is controlled by the extracellular calcium ion concentration.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 758-759 
    ISSN: 1420-9071
    Schlagwort(e): Crickets ; corpus allatum activity ; juvenile hormone III ; temperature ; reproduction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In the Mediterranean field cricket,Gryllus bimaculatus, reproduction is controlled by temperature and the corpus allatum (CA) hormone JH III. In CA of females reared at 24°∶12°C(16∶8 h) (high reproduction rate) a first peak in JH III synthesis is reached about 4 days earlier than in those of 20°C females (low reproduction rate). Furthermore, in 20°C animals CA activity is low during the entire oviposition period, whereas at 24°∶12°C high CA activity is found during this period of adult life. The results indicate a stimulation of CA activity and reproduction by thermoperiods around a constant low temperature.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 997-1001 
    ISSN: 1420-9071
    Schlagwort(e): Myosin light chain kinase ; calcium ; c-AMP ; calmodulin ; smooth muscle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1048-1051 
    ISSN: 1420-9071
    Schlagwort(e): Na, K-ATPase ; calcium ; calmodulin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The effect of calcium on Na, K-ATPase activity of rat brain homogenates and its modification by the chelating agent EDTA has been investigated. In the absence of EDTA, free calcium (approximately 10−6mol/l) stimulates Na,K-ATPase activity; in the presence of EDTA the same concentration of free calcium is without effect on the enzyme. In the absence of EDTA the stimulation by calcium of Na,-K-ATPase activity is enhanced by the additional presence of calmodulin but in the presence of EDTA, even when calmodulin is added to excess, calcium still fails to stimulate the enzyme. The possibility that EDTA interferes with an interaction between a calcium-calmodulin complex and Na,K-ATPase is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    ISSN: 1420-9071
    Schlagwort(e): Smooth muscle ; calcium ; myosin light chain kinase ; regulation of contraction ; ATPase ; mechanics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The contraction induced by a Ca2+-independent myosin light chain kinase (MLCK-) was characterized in terms of isometric force (Fo), immediate elastic recoil (SE), unloaded shortening velocity (Vus), shortening under a constant load and ATPase activity of chemically skinned smooth muscle preparations. These parameters were compared to those measured in a Ca2+-induced contraction to assess the nature of cross bridge interaction in the MLCK-induced contraction. Fo developed in chicken gizzard fibers as well as SE were similar in contractions elicited by either agent. Vus in the contraction induced by MLCK-(0.36 mg/ml) was similar though averaged 39.3±8.9% less than Vus induced by Ca2+ (1.6x10−6M) in the control fibers. Addition of Ca2+ (1.6x10−6M) to a contraction induced by MLCK-resulted in small increases in both Fo and Vus. Shortening under a constant load was similar for both types of contractions. The contraction induced by MLCK-was accompanied by an increased rate of ATP hydrolysis. The MLCK-induced contraction is thus kinetically similar though not identical to a contraction induced by Ca2+. We conclude that with respect to actin-myosin interaction, MLCK- and Ca2+-induced contractions are similar.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1020-1025 
    ISSN: 1420-9071
    Schlagwort(e): Smooth muscle energetics ; light chain phosphorylation ; crossbridges ; calcium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Conclusion On the basis of measurements of the high energy phosphate usage associated with different mechanical states, as well as the degree of myosin light chain phosphorylation and mechanical properties, information has been gained concerning the existence and regulation of different crossbridge states in smooth muscle. Although incomplete, a general operational scheme is shown in figure 5. At very low intracellular calcium concentrations, actin and myosin are dissociated, as shown by a loss of resistance to stretch in resting muscles. At somewhat higher intracellular calcium concentrations in atonic, resting muscles, crossbridges can attach and be manifest mechanically as an increased resistance to stretch without ATP-driven crossbridge cycling and active force production. When the muscle is activated, intracellular calcium increases further, the light chains of myosin are phosphorylated through the calcium-calmodulin activation of myosin light chain kinase, actin-activated myosin ATPase activity increases and crossbridges cycle. Calcium also appears to modulate the ATPase activity and the rate of cycling of the phosphorylated crossbridge. The crossbridge cycling rate is highest during force development and slows with time as maximum isometric force is maintained reflecting a change in the rate at which phosphorylated crossbridges cycle. This may result from a decrease in the intracellular free calcium concentration with continued stimulation. During relaxation, the intracellular calcium concentration decreases, there is net dephosphorylation of the myosin light chains, the rate at which phosphorylated crossbridges cycle slows further with a gradual return to the attached, but non-cycling state or the detached state.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 29 (1985), S. 193-197 
    ISSN: 1432-1041
    Schlagwort(e): Glibenclamide ; intestinal absorption ; small and large intestine ; bioavailability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary In a study of eight volunteers and six patients, glibenclamide was placed at different sites of the gastro-intestinal tract under visual control. The dose was instilled once into the stomach and once into the duodenum of the eight volunteers in a randomized crossover design. The six patients underwent diagnostic colonoscopy, and the dose was placed into the ascending colon if pathological findings were not present. The area under the concentration-time curve, completed by extrapolation, and the mean residence time of the drug in the body were calculated. These pharmacokinetic characteristics were examined using a Jonckheere test for ordered alternatives and a Wilcoxon signed rank pair test. The means of the areas under the curve were 477±131 ng·h ml−1 for the stomach, 475±142 ng·h ml−1 for the duodenum and 486±301 ng·h ml−1 for the colon. The mean residence time changed from 2.67±0.35 h for the stomach to 2.42±0.48 h for the duodenum and 3.55±0.68 h for the colon. These results indicate that although glibenclamide is absorbed from all three sites of the gastro-intestinal tract to the same extent, the rates of absorption are different. It is discussed whether these findings really confirm the pH-partition hypothesis in drug absorption. Since glibenclamide — a weak acid — has a pK-value of about 6.5, these data seem to confirm the pH-partition hypothesis of drug absorption.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 29 (1985), S. 251-253 
    ISSN: 1432-1041
    Schlagwort(e): paracetamol ; antacids ; acetaminophen ; bioavailability ; kinetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary The effect of two antacids on the bioavailability of paracetamol has been investigated in 12 young healthy volunteers. Following a random cross over design, each subject swallowed, on three separate occasions, one weak apart, 500 mg paracetamol alone, or together with two different aluminium hydroxide, magnesium hydroxide preparations (Dimalan and Maalox). Plasma paracetamol levels were measured by HPLC. The bioavailability of paracetamol was not altered by either antacid, but they both delayed the time to peak plasma concentration (0.85 h; 1.43 h; 1.25 h, without antacid, with Dimalan and with Maalox respectively). The peak plasma concentration was not affected by concurrent antacid administration.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 28 (1985), S. 229-230 
    ISSN: 1432-1041
    Schlagwort(e): diazepam ; dipotassiumchlorazepate ; benzodiazepines ; bioavailability ; administration ; healthy volunteers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary Dipotassium chlorazepate (DPC) and diazepam (DZM) were given i.m. and i.v. to 6 healthy volunteers in doses of 20 mg (48.9 µmol) DPC and 15 mg (52.0 µmol) DZM. The interval between the injections was at least 1 week. Plasma samples were analyzed for DPC and DZM by HPLC. The bioavailability of DPC and DZM after i.m. administration, determined from computer calculated AUCs, was 1.04 and 0.85, respectively.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 16
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 28 (1985), S. 89-95 
    ISSN: 1432-1041
    Schlagwort(e): moclobemide ; Ro 11-1163 ; pharmacokinetics ; bioavailability ; MAO activity in platelets ; monoamine metabolites in urine ; healthy volunteers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary The plasma concentrations of the MAO-inhibitor moclobemide (Ro 11-1163) were determined in six healthy male subjects after oral (tablets) administration. Effects on MAO activity in platelets and excretion of monoamine metabolites in urine were investigated. The design of the study was a double-blind cross-over study with single oral doses of placebo, 50, 100 and 200 mg of moclobemide. The elimination profile of the drug showed that the half life of the unchanged drug ranged between 1 and 2 h except in one subject with a half-life of about 4 h. The mean bioavailability calculated using flow model concepts was F=0.43 after 50 mg, F=0.47 after 100 mg and F=0.59 after 200 mg. The outlier with a t1/2 of 4 h was found to have a bioavailability of more than 0.80 after all 3 doses. The slightly increasing bioavailability with higher doses was interpreted as evidence of saturable hepatic first-pass elimination of the drug. MAO activity in platelets was measured before and 2, 6 and 24 h after drug administration. No inhibition of platelet MAO was obtained at any point in time or dose level, as to be expected since moclobemide preferentially inhibits MAO A. Urine excretion of the monoamine metabolites homovanillic acid (HVA), dihydroxyphenylacetic acid (DOPAC), 3-methoxy-4-hydroxy-phenylglycol (MOPEG) and 5-hydroxyindoleacetic acid (5-HIAA) was followed during 48 h after placebo, 50 and 200 mg of moclobemide. Time but not dose contributed significantly to the variability in excretion of the monoamine metabolites. An apparent reduction of HVA and DOPAC levels was obtained in the early phase after the administration of 200 mg of moclobemide. In 1 subject with a mild drug reaction a pronounced decrease in the levels of all the metabolites was obtained. In the other 5 subjects, the compound was very well tolerated with a few reported side-effects like increased activity, somnolence or sweatings. There was a slight but significant increase in blood pressure following 50 and 100 mg but not 200 mg of moclobemide.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 17
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 28 (1985), S. 53-59 
    ISSN: 1432-1041
    Schlagwort(e): furosemide ; respiratory failure ; furosemide glucuronide ; first-pass metabolism ; diuretic effect ; bioavailability ; food effect ; chronic treatment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary The bioavailability and diuretic effect of furosemide 40 mg administered orally for at least 6 months have been compared in patients with chronic respiratory failure and in healthy controls. The mean urinary recovery of unchanged drug was 11.5 mg and 9.41 mg in 24 h after pre- and postprandial administration to 10 patients, whereas the recovery was 14.4 mg in 10 healthy subjects. The diuretic effect, in terms of urine flow and sodium ion excretion in the 6 h after administration, was also less in patients than in healthy subjects. This was ascribed to the lower bioavailability of furosemide in patients, based on the urinary recovery of unchanged drug, and not to a lower level of response to furosemide than in healthy subjects. The mean absolute bioavailability of furosemide in 6 patients was 41.3% and 63.4%, respectively, calculated from unchanged drug and total drug (unchanged plus glucuronide conjugate). Approximately 53.9% of the dose of furosemide was excreted as the glucuronide conjugate after oral administration, and 34.2% after i.v. injection in the 6 patients. In 3 of the 6 patients studied, a distinct first-pass effect for glucuronidation of furosemide was observed after oral administration. In another study, the mean glucuronide fraction recovered in 24-h urine was 20.7% and 7.3% (p〈0.01) in 38 patients and 12 healthy subjects, respectively. The fraction in urine was not affected by changing the dose of furosemide from 20 to 120 mg. The lower bioavailability in patients as compared to healthy subjects is ascribed to enhanced glucuronidation and incomplete drug absorption.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 18
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 28 (1985), S. 85-88 
    ISSN: 1432-1041
    Schlagwort(e): bioavailability ; distribution-free statistical method ; confidence limits
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary The confidence interval approach to bioavailability assessment depends first on selection of the confidence level, usually 95%, and then determination of the confidence limits for the expected bioavailability ratio AUC(Test)/AUC(Reference). In practice, however, it is sometimes of greater interest to know the probability that the expected bioavailability will fall below a critical value, for example 0.75, or within a clinically set bioequivalence range, for example 0.80 to 1.25. Up to now, posterior probability distributions have been suggested, based on classical analysis of variance (ANOVA) with its rather restrictive assumptions, including that of a (logarithmic) normal distribution. In this report, a distribution-free confidence interval based on the Wilcox-on signed-rank statistic has been generalized so that confidence probabilities can be obtained for any given confidence limits. In the case of unimodal and almost symmetrical sampling distributions, the results obtained are very similar to those of the ANOVA-based posterior probability distribution. However, skewed or multimodal sampling distributions are better reflected by the proposed distribution-free method, and more valid information is obtained in these cases, as demonstrated by examples.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 27 (1985), S. 637-644 
    ISSN: 1432-1041
    Schlagwort(e): isosorbide-dinitrate ; pharmacokinetics ; analytical method ; bioavailability ; drug metabolism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary The bioavailabilities of a conventional and two slow release 20 mg isosorbide dinitrate (ISDN) formulations were compared after oral administration in a three way cross-over study in 8 male volunteers. In a further group of 6 male volunteers the pharmacokinetics and metabolism of ISDN were investigated after intravenous infusion of a median dose of 14.1 mg for 2.5 h. A new analytical procedure was developed for the determination of isosorbide-5-mononitrate-2-glucuronide (IS-5-MN-2-Glu) and of isosorbide (IS). Kinetic data analysis on a molar basis was performed by the program package KINPAK providing model independent parameters. The median elimination half-lives of ISDN, IS-5-MN, IS-2-MN and IS-5-MN-2-Glu were 0.7, 5.1, 3.2 and 2.5 h, respectively. The systemic clearance of ISDN was 3.7 l/min and the distribution volume 2521 (3.1 l/kg). Apart from IS-5-MN-2-Glu, with a renal clearance of 5.9 l/min which suggested substantial glucuronidation in the kidney, the renal clearances of ISDN, IS-5-MN, IS-2-MN and the corresponding amounts excreted were negligible. 27.8% of the administered ISDN was excreted as IS-5-MN-2-Glu (8.7%) and IS (19.1%). Calculations based on the two mononitrate metabolites formed from ISDN showed an incomplete recovery of 84.1%, leading to the assumption that a simultaneous denitration to IS must have occurred. The rate of denitration at each nitro group in ISDN was almost twice as high as for the same position in the corresponding mononitrate. The bioavailability of the conventional ISDN formulation was 19%, although complete absorption was indicated by comparison of the percentages of mononitrate metabolites formed after the different routes of administration. On the same basis the absorption of the two sustained release formulations was found to be poor.
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  • 20
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 29 (1985), S. 109-113 
    ISSN: 1432-1041
    Schlagwort(e): griseofulvin ; skin blister fluid levels ; pharmacokinetics ; healthy subjects ; bioavailability ; protein binding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary Griseofulvin and 6-demethylgriseofulvin (6-DMG) in plasma, suction blister fluid (SBF) and cantharides blister fluid (CBF) and urinary excretion of 6-DMG, were evaluated following administration of single oral doses of an ultramicrosize and a microsize formulation of griseofulvin to 6 healthy volunteers. The bioavailability of griseofulvin was higher following the ultramicrosize formulation when 64% of the dose was recovered (via metabolites) versus 52% after the microsize preparation. Penetration into skin blister fluid was delayed as compared to plasma levels; the peak concentration in plasma was observed at 3–4 h, whereas griseofulvin in CBF increased up to 6 h. The terminal half-live was calculated from plasma levels to 9.3 h. The half-lives calculated from SBF and CBF concentrations were 9.2 and 9.8 h, respectively, (n=5). In plasma 84% of griseofulvin was bound to proteins, predominantly to albumin; binding in SBF and CBF was 72 and 82%, respectively. 3 h after drug administration the free concentration in plasma significantly exceeded the free concentrations in SBF and CBF. Distribution equilibrium between plasma and skin blister fluid was observed after 27 h. Thus, during chronic administration, the plasma griseofulvin level should reflect its concentration in the target organ.
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  • 21
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 29 (1985), S. 351-354 
    ISSN: 1432-1041
    Schlagwort(e): griseofulvin ; skin blister fluid ; plasma concentration ; blister fluid concentration ; pharmacokinetics ; microsize formulation ; urinary excretion ; bioavailability ; different formulations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary Griseofulvin was administered orally to 6 healthy volunteers for 6 days. The subjects received 500 mg of a microsize formulation and 330 mg of an ultramicrosize formulation, according to a cross-over design. The drug was determined in plasma, suction blister fluid (SBF) and cantharides blister fluid (CBF) following the last dose. Urinary excretion of the main metabolites 6-demethylgriseofulvin (6-DMG) and its glucuronic acid conjugate was also measured. The pharmacokinetic parameters were compared with those obtained from a recent single dose experiment. On repeated administration, the bioavailability of griseofulvin was significantly lower from the microsize formulation; the urinary recovery of total 6-DMG was 33.8% versus 53.6% on administration of the ultramicrosize material. Bioavailability was reduced as compared to ingestion of a single dose. The reduction was more prominent following the microsize (36%) than the ultramicrosize (17%) formulation. Penetration into skin blister fluid was not altered as compared to the single dose experiment. Relative areas under the blister fluid-time curves amounted to 51% (SBF) and 80% (CBF) of the area under the plasma level-time curve. The concentration of unbound griseofulvin in these body fluids was identical throughout the entire dosage interval. Unbound griseofulvin levels were low in comparison with the minimum inhibitory concentrations for strains of trichophyton and microsporum.
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 29 (1985), S. 379-381 
    ISSN: 1432-1041
    Schlagwort(e): confidence intervals ; bioavailability ; Bayesian analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Materialart: Digitale Medien
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  • 23
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 29 (1985), S. 477-481 
    ISSN: 1432-1041
    Schlagwort(e): budesonide ; glucocorticoid ; nasal administration ; pharmacokinetics ; bioavailability ; systemic effects
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary Budesonide, a topically potent glucocorticoid, was administered to 4 healthy volunteers by i.v. infusion and by nasal instillation of 100 µg tritium-labelled drug. Plasma was analyzed by liquid chromatography plus scintillation counting of collected fractions. After i.v. administration the plasma clearance was 0.92 l/min and the apparent volume of distribution was 2.8 l/kg. After nasal administration, the time to reach the peak plasma level was approximately 30 min, and the systemic availability was 102%. Budesonide had marginal effects on plasma cortisol and white blood cell counts either after i.v. or nasal administration. Thus, nasally instilled budesonide in solution is rapidly and completely absorbed from the nasal mucosa. The systemic effects after this clinically recommended nasal dose were negligible.
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  • 24
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 28 (1985), S. 411-417 
    ISSN: 1432-1041
    Schlagwort(e): ketanserin ; ketanserinol ; pharmacokinetics ; age ; healthy volunteers ; bioavailability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary The bioavailability of ketanserin has been examined in a cross-over experiment in 21 elderly subjects (aged 59–72 years) by administration of tablets (40 mg), solution (40 mg) and injectable solution (10 mg). After two weeks of treatment with 40 mg ketanserin tablets further 18 blood samples for analysis were collected under steady-state conditions. Plasma levels were measured by HPLC. The absolute bioavailability of ketanserin tablets was 52.7%; their relative bioavailability compared to a solution containing an equal quantity of active compound was 85.5%. Therefore, the low absolute bioavailability of ketanserin cannot be attributed to the formulation. The active compound was rapidly liberated from the tablet, reaching a peak of 103.8 ng/ml after 0.97 h. Individual plasma level-time curves were fitted to an open three compartment model and a half-life of 17.7±7.26 h was calculated for the terminal elimination phase. An average terminal elimination half-life of 15.4±4.2 ng/ml was found after administration of the ketanserin solution. Multiple dosing with 40 mg tablets b.d.s. resulted in an AUC over one dosing interval at steady-state of 666±201 ng × h/ml. The AUC extrapolated to infinity was 1200±405 ng × h/ml for the last tablet. This is 1.8-times the AUC in one dosing interval, and 2.3-times the AUC of a single dose. Under steady-state conditions, the mean peak plasma level was 155.1 ng/ml (1.08 h after dosing) and the terminal half-life was 19.1±5.1 h. For the metabolite ketanserinol terminal half-lives of 21.4 h after a single tablet and 31.0 h after discontinuation of multiple dosing were calculated. Compared to the parent compound there was much more marked accumulation of ketanserinol. Despite moderate accumulation and prolongation of the terminal half-life of ketanserin under steady-state conditions, dosage adjustment is not required in elderly people. First-pass metabolism and bioavailability remained in the range found in previous studies of ketanserin in young subjects.
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  • 25
    Digitale Medien
    Digitale Medien
    Springer
    European journal of clinical pharmacology 28 (1985), S. 581-583 
    ISSN: 1432-1041
    Schlagwort(e): piretanide ; liver cirrhosis ; ascites ; bioavailability ; pharmacokinetics ; diuretic
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie , Medizin
    Notizen: Summary The pharmacokinetics of piretanide, a new loop diuretic, were studied in seven patients with severe liver disease before and after resolution of ascites. The time to maximum concentration was significantly prolonged by the presence of ascites. Tmax after relief of ascites was similar to that seen for normal volunteers. Area under the curves, bioavailability, volumes of distribution and elimination half-lives did not change after resolution of the ascites: two patients in whom diuretic resistant ascites occurred showed similar pharmacokinetics to that of the diuretic responders. Reduced responsiveness to piretanide therapy in patients with gross ascites does not appear to be the result of decreased bioavailability.
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  • 26
    ISSN: 1432-072X
    Schlagwort(e): Phototrophic bacteria ; Rhodospirillaceae ; Glutamine synthetase ; Nitrogen metabolism ; Nitrogen fixation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The phylogenetically related phototrophic bacteria Rhodospirillum tenue and Rhodocyclus purpureus modulate activity of their glutamine synthetases by adenylylation/deadenylylation. Evidence for covalent modification includes the inhibitory effect of Mg2+ on the activity of glutamine synthetase extracted from cells of either species grown on excess ammonia, and the lack of Mg2+ inhibition of activity of the enzyme isolated from N2-(R. tenue) or glutamine (R. purpureus)-grown cells. In addition, snake venom phosphodiesterase treatment of glutamine synthetase from either species grown on excess ammonia relieved Mg2+ inhibition of the enzyme (as measured via the γ-glutamyl transferase assay), and changed the cation specificity from Mn2+ to Mg2+ (in the biosynthetic assay).
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  • 27
    ISSN: 1432-072X
    Schlagwort(e): Ammonium assimilation ; Excretion ; Anabaena azollae ; Azolla caroliniana ; Cyanobacteria ; Glutamine ; Glutamate formation ; Nitrogen fixation ; Symbiosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Anabaena azollae was isolated fromAzolla caroliniana by the “gentle roller” method and differential centrifugation. Incubation of suchAnabaena preparations for 10 min with [13N]N2 resulted in the formation of four radioactive compounds; ammonium, glutamine, glutamate and alanine. Ammonium accounted for 66% of the total radioactivity recovered and 58% of the ammonium was in an extracellular fraction. Since essentially no extracellular13N-labeled organic compounds were found, it appears that ammonium is the compound most probably made available toAzolla during dinitrogen-dependent growth of the association. The kinetics of incorporation of exogenous13NH 4 + into glutamine and glutamate were characteristic of a precursor (glutamine)-product (glutamate) relationship and consistent with assimilation by the glutamine synthetase-glutamate synthase pathway. The results of experiments using the glutamine synthetase inhibitor, methionine sulfoximine, the glutamate synthase inhibitor, diazo-oxonorleucine, and increasing the ammonium concentration to greater than 1 mM, provided evidence for assimilation primarily by the glutamine synthetase-glutamate synthase pathway with little or no contribution from biosynthetic glutamate dehydrogenase. While showing that N2 fixation and NH 4 + assimilation were not tightly coupled metabolic processes in symbioticAnabaena, these results reflect a composite picture and do not indicate the extent to which ammonium assimilatory enzymes might be regulated in filaments associated with specific stages in theAzolla-Anabaena developmental profile.
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  • 28
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 142 (1985), S. 333-339 
    ISSN: 1432-072X
    Schlagwort(e): Photosynthesis ; Membrane structure ; Electron microscopy ; Photosynthetic bacteria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The organization of photosynthetic membranes in the cytoplasm of the photosynthetic bacterium Rh. viridis has been examined by several techniques for electron microscopy. Thin sections of membrane stacks show that the regular lattice of membrane subunits reported in other studies can be observed in thin section. Tilting of sections in the electron microscope shows that the regular lattices of several membranes overlap in a way that suggests they are in register with each other. This observation can be confirmed by freeze-fracture images in which a regular arrangement of membrane lattices can be observed, each perfectly aligned. Analysis of the spacings of membrane pairs shows that the photosynthetic membranes of Rh. viridis are very closely apposed. The mean diameter of two membranes is 160A, and the average space between two such membranes is only 42A. When a recently developed atomic level model of Rh. viridis reaction center is superimposed against these spacings, each reaction center extends from the surface of its respective membrane far enough to make contact with an apposing membrane. The limited free space between membranes and regular alignment of lattices has a number of implications for how this membrane is organized to carry out the process of energy transfer.
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  • 29
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 143 (1985), S. 72-76 
    ISSN: 1432-072X
    Schlagwort(e): Oscillatoria ; Cyanobacteria ; Nitrogen fixation ; Oxygen protection of N2-ase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Oscillatoria sp. strain 23 is a filamentous, non-heterocystous cyanobacterium that fixes nitrogen aerobically. Although, in this organism nitrogenase is inactivated by oxygen a high tolerance is observed. Up to a pO2 of 0.15 atm, oxygen does not have any measurable effects on acetylene reduction. Higher concentrations of oxygen inhibited the activity to a relatively high degree. Evidence for two mechanisms of oxygen protection of nitrogenase in this cyanobacterium was obtained. A high rate of synthesis of nitrogenase may allow the organism to maintain a certain amount of active enzyme under aerobic conditions. Secondly, a switch off/on mechanism may reversibly convert the active enzyme into a non-active form which is insensitive to oxygen inactivation after a sudden and short-term exposure to high oxygen concentrations. It is conceived that these mechanisms in addition to a temporal separation of nitrogen fixation from oxygenic photosynthesis sufficiently explain the regulation process of aerobic nitrogen fixation in this organism.
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  • 30
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 143 (1985), S. 67-71 
    ISSN: 1432-072X
    Schlagwort(e): Oscillatoria ; Cyanobacteria ; Nitrogen fixation ; Light-dark cycles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The non-heterocystous cyanobacterium Oscillatoria sp. strain 23 fixes nitrogen under aerobic conditions. If nitrate-grown cultures were transferred to a medium free of combined nitrogen, nitrogenase was induced within about 1 day. The acetylene reduction showed a diurnal variation under conditions of continuous light. Maximum rates of acetylene reduction steadily increased during 8 successive days. When grown under alternating light-dark cycles, Oscillatoria sp. fixes nitrogen preferably in the dark period. For dark periods longer than 8 h, nitrogenase activity is only present during the dark period. For dark periods of 8 h and less, however, nitrogenase activity appears before the beginning of the dark period. This is most pronounced in cultures grown in a 20 h light – 4 h dark cycle. In that case, nitrogenase activity appears 3–4 h before the beginning of the dark period. According to the light-dark regime applied, nitrogenase activity was observed during 8–11 h. Oscillatoria sp. grown under 16 h light and 8 h dark cycle, also induced nitrogenase at the usual point of time, when suddenly transferred to conditions of continuous light. The activity appeared exactly at the point of time where the dark period used to begin. No nitrogenase activity was observed when chloramphenicol was added to the cultures 3 h before the onset of the dark period. This observation indicated that for each cycle, de novo nitrogenase synthesis is necessary.
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  • 31
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 141 (1985), S. 40-43 
    ISSN: 1432-072X
    Schlagwort(e): Nitrogenase ; Nitrogen fixation ; Regulation ; Photosynthetic bacteria ; Chromatium ; Ammonia switch off
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Nitrogenase in Chromatium vinosum was rapidly, but reversibly inhibited by NH 4 + . Activity of the Fe protin component of nitrogenase required both Mn2+ and activating enzyme. Activating enzyme from Rhodospirillum rubrum could replace Chromatium chromatophores in activating the Chromatium Fe protein, and conversely, a protein fraction prepared from Chromatium chromatophores was effective in activating R. rubrum Fe protein. Inactive Chromatium Fe protein contained a peptide covalently modified by a phosphate-containing molecule, which migrated the same in SDS-polyacrylamide gels as the modified subunit of R. rubrum Fe protein. In sum, these observations suggest that Chromatium nitrogenase activity is regulated by a covalent modification of the Fe protein in a manner similar to that of R. rubrum.
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  • 32
    ISSN: 1432-072X
    Schlagwort(e): Bacteriolysis ; Penicillin ; Autolysis ; Cell wall ; Electron microscopy ; Staphylococcus aureus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The actual reason for the penicillin-induced bacteriolysis of staphylococci was shown to be the “punching” of one or a few minute holes into the peripheral cell wall at predictable sites. These perforations were the result of the lytic activity of novel, extraplasmatic vesicular structures, located exclusively within the bacterial wall material, which we have named “murosomes”. In untreated staphylococci the punching of holes into the peripheral wall is a normal process which follows cross wall completion and represents the first visible step of cell separation. Under penicillin, however, analogous holes are punched by the murosomes at sites of presumptive cell separation even if no sufficient cross wall material had been assembled before at this site (but had rather been deposited at other sites). Consequently, because of the internal pressure of the protoplast, lytic death is the inevitable result of this perforation of the protective peripheral wall. Hence, the real mechanism of penicillin-induced bacteriolysis in staphylococci is considered to be mainly the result of a special morphogenetic wall defect: bacteriolysis is taking place regularly when a cell separation process is no longer preceeded by sufficient cross wall assembly at the correct place. However, hypotheses which are based purely on some variations of overall biochemical processes like total wall enzyme activities or total wall synthesis are not regarded to be sufficient to explain this type of lytic death.
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  • 33
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 142 (1985), S. 259-261 
    ISSN: 1432-072X
    Schlagwort(e): Methanogenic bacteria ; Plasmid isolation ; Alkaline lysis ; CsCl gradient ; Restriction endonuclease mapping ; Electron microscopy ; DNA homology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Of 21 recently isolated strains of methanococci, one was found to harbor a small, cryptic, low copy number plasmid. Reproducible recovery was achieved by alkaline lysis of cells pretreated with proteinase K in an osmotically stabilizing buffer. The plasmid was found to contain a singleAval site. No homology was detected between the plasmid and DNA from any of the other new strains or from five known species of methanococci.
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 143 (1985), S. 185-191 
    ISSN: 1432-072X
    Schlagwort(e): Heterocyst ; Pyruvate: ferredoxin oxidoreductase ; Nitrogen fixation ; Electron transport to nitrogenase ; Ferredoxin ; Cyanobacteria ; Anabaena cylindrica ; Anabaena variabilis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Various electron donors were found to stimulate C2H2 reduction (N2 fixation) by isolated heterocysts from Anabaena variabilis and Anabaena cylindrica. Intermediates of glycolysis and the tricarboxylic acid cycle as well as unphosphorylated sugars like glucose, fructose and erythrose were among these electron donors. The transfer of electrons from donors like H2, NADH, glyoxylate and glycollate was strictly light-dependent, whereas others like NADPH or pyruvate plus coenzyme A supported C2H2 reduction also in the dark. In all cases, the overall activity was enhanced by light. The stimulation by light was more distinct with heterocysts from A. variabilis than with heterocysts from A. cylindrica. The present communication establishes that pyruvate supports C2H2 reduction by heterocysts from either A. variabilis or A. cylindrica with rates comparable to those with other electron donors. Pyruvate could, however, support C2H2 reduction only in the presence of coenzyme A, and the concentrations of both coenzyme A and pyruvate were crucial. A pyruvate-dependent reduction of ferredoxin by extracts from heterocysts was recorded spectrophotometrically. Glyoxylate, which is an inhibitor of thiamine pyrophosphate-dependent decarboxylations, inhibited pyruvate-dependent C2H2 reduction. This result supports the conclusion that pyruvate is metabolised by pyruvate: ferredoxin oxidoreductase in heterocysts. High concentrations of pyruvate and other electron donors inhibited C2H2 reduction which suggests that nitrogenase activity in heterocysts may be controlled by the availability of electron donors.
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  • 35
    ISSN: 1432-072X
    Schlagwort(e): Alfalfa ; Conjugation ; Cross inoculation ; Host specificity ; Hydrogen uptake ; Nodulation ; Nitrogen fixation ; Rhizobium ; Plasmids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract pIJ1008, a Rhizobium leguminosarum plasmid which determines hydrogen uptake ability and symbiotic functions in pea was transferable to three of seven natural isolates of R. meliloti tested. In these three strains, pIJ1008 was maintained stably with the respective sym megaplasmid indigenous to each R. meliloti strain. These strains carrying both plasmids nodulated alfalfa but not pea. By reisolation and examination of the strains from alfalfa nodule tissue, it was shown that pIJ1008 continued to be maintained but that pea-nodulation ability was suppressed. In one strain of R. meliloti which carries a 200 kb cryptic plasmid (in addition to a megaplasmid), the transfer and selection for pIJ1008 resulted in the loss of the cryptic plasmid. In three separate plant growth experiments, alfalfa nodules induced by each of the R. meliloti strain carrying both sym plasmids were assayed for hydrogen uptake activity. The average activity was 40-, 3.5-and 2-fold higher than with the respective pIJ1008-free strains. However, this higher activity was not accompanied by an increase in plant biomass or nitrogen content of shoots.
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  • 36
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 140 (1985), S. 338-342 
    ISSN: 1432-072X
    Schlagwort(e): Sporosarcina halophila ; Endospores ; Electron microscopy ; Heat resistance ; Ethanol resistance ; Germination ; Dipicolinic acid
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Sporosarcina halophila forms endospores. Electron micrographs revealed ultrastructural similarity to spores of S. ureae. Spore germination indicated by loss of refractility, darkening, swelling and formation of new vegetative cells was followed by phase contrast light microscopy. To induce spore germination, the endospores needed to be heat avtivated. After activation, they were inoculated into nutrient broth medium supplemented with sea-water. Double concentrated sea-water was found to be optimal for germination. Similar to other bacterial endospores, the spores were found to be resistant to heat and ethanol. An ultraviolet absorbing substance was isolated from suspensions of free spores; it was identified to be pyridine-2,6-dicarboxylic acid (DPA) usually present in bacterial spores. DPA was detected in amounts ranging from 5–7% of the spore dry weight; it was not detected in extracts of vegetative cells.
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  • 37
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 141 (1985), S. 85-90 
    ISSN: 1432-072X
    Schlagwort(e): C. sporosphaeroides ; Citrate lyase ; Regulation ; Purification ; Properties ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cells of Clostridium sporosphaeroides which were grown on citrate contained citrate lyase and citrate lyase acetylating enzyme, but no detectable citrate synthase and citrate lyase deacetylase activities. Citrate lyase from C. sporosphaeroides was purified to homogeneity as judged by polyacrylamide gel electrophoresis and high performance liquid chromatography. In contrast to the enzyme from Clostridium sphenoides, the addition of l-glutamate was not necessary for activity and stabilization of the enzyme. The purified enzyme had a specific activity of 34 U/mg protein and was comparable to other citrate lyases with respect to its molecular weight and subunit composition. Electron microscopic investigations showed that similar to the lyase from C. sphenoides and in contrast to all other citrate lyases examined so far, the majority of the enzyme molecules was present in “star” form.
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  • 38
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 142 (1985), S. 289-294 
    ISSN: 1432-072X
    Schlagwort(e): Proteus mirabilis ; Nitrogen fixation ; nif genes ; nif plasmids ; Klebsiella pneumoniae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Self-transmissible plasmids carryinghis andnif genes fromKlebsiella pneumoniae have been introduced into threehis mutants ofProteus mirabilis: strains 5006-1, WR19 and WR20. Expression ofhis by the transconjugants was unequivocal, if slightly temperature-sensitive, but none was Nif+ when tested for acetylene reduction in anaerobic glucose medium using inocula from rich or glucose-minimal aerobic agar cultures. Succinate or pyruvate in place of glucose, low glucose, lower temperature or elevated Na2MoO4 did not allownif expression and no nitrogenase MoFe-protein peptide was detected immunologically after exposure to conditions in which diazotrophic enterobacteria, normal or genetically constructed, derepressnif. One strain,P. mirabilis WR19, carrying thehis nif Kmr plasmid pMF250 was examined in detail. Thenif activator genenifA was introduced on the plasmid pCK1. Such derivatives remained Nif- when tested, after aerobic growth on rich agar media, with normal or low glucose, with succinate or with elevated Mo. However, pre-conditioning by aerobic growth on glucose-minimal agar led to subsequent anaerobic expression ofnif in glucose medium from pMF250 in WR19 carrying pCK1. NH 4 + or proline could serve as N-source in the glucose-minimal agar. Maximum activity was about 5% of that ofK. pneumoniae in our assay conditions. Material cross-reacting with anti-serum to the nitrogenase MoFe protein was formed. Nitrogenase activity was not ‘switched off’ by NH 4 + .P. mirabilis WR19 (pCK1) showed NH 4 + -constitutive temperature-sensitive kanamycin resistance (anif-related phenotype of this plasmid) in aerobic glucose minimal medium. Expression ofnif inP. mirabilis WR19 (pCK1, pMF250) was NH 4 + -repressible despite the constitutivenifA character of pCK1 and introduction of thentrA + plasmid pMM17 did not alter this phenotype. However, pCK1 did not give rise to NH 4 + -constitutive diazotrophy in the wild-typeK. pneumoniae M5al. A construct of WR19 carrying pMF250 and constitutiventrC plasmid (pMD45) remained Nif- even after pre-growth on glucose-minimal media. We conclude (a) thatP. mirabilis forms a gene product functionally equivalent to that ofntrA inK. pneumoniae, (b) that it forms no functional equivalent of thentrC product in our growth conditions. The need for pre-conditioning on aerobic glucose media remains perplexing.
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  • 39
    ISSN: 1432-072X
    Schlagwort(e): Evolution ; Nif genes ; Nitrogen fixation ; Nitrogenase ; Nucleotide sequence ; Phylogeny ; Rhizobium ; 16S rRNA cataloguing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract It was known that nitrogenase genes and proteins are well conserved even though they are present in a large variety of phylogenetically diverse nitrogen fixing bacteria. This has lead to the speculation, among others, that nitrogen fixation (nif) genes were spread by lateral gene transfer relatively late in evolution. Here we report an attempt to test this hypothesis. We had previously established the complete nucleotide sequences of the three nitrogenase genes from Bradyrhizobium japonicum, and have now analyzed their homologies (or the amino acid sequence homologies of their gene products) with corresponding genes (and proteins) from other nitrogen fixing bacteria. There was a considerable sequence conservation which certainly reflects the strict structural requirements of the nitrogenase iron-sulfur proteins for catalytic functioning. Despite this, the sequences were divergent enough to classify them into an evolutionary scheme that was conceptually not different from the phylogenetic positions, based on 16S rRNA homology, of the species or genera harboring these genes. Only the relation of nif genes of slow-growing rhizobia (to which B. japonicum belongs) and fast-growing rhizobia was unexpectedly distant. We have, therefore, performed oligonucleotide cataloguing of their 16S rRNA, and found that there was indeed only a similarity of S AB=0.53 between fast- and slowgrowing rhizobia. In conclusion, the results suggest that nif genes may have evolved to a large degree in a similar fashion as the bacteria which carry them. This interpretation would speak against the idea of a recent lateral distribution of nif genes among microorganisms.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 40
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 141 (1985), S. 105-111 
    ISSN: 1432-072X
    Schlagwort(e): Ammonia ; Anabaena ; Cyanobacteria ; Nitrogen fixation ; Nitrogenase ; Nostoc
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Incubation in the dark of photoautotrophically grown N2-fixing heterocystous cyanobacteria leads to a loss of nitrogenase activity. Original levels of nitrogenase activity are rapidly regained upon re-illumination of the filaments, in a process dependent on de novo protein synthesis. Ammonia, acting indirectly through some of its metabolic derivatives, inhibits the light-promoted development of nitrogenase activity in filaments of Anabaena sp. ATCC 33047 and several other cyanobacteria containing mature heterocysts. The ammonia-mediated control system is also operative in N2-fixing filaments in the absence of any added source of combined nitrogen, with the ammonia resulting from N2-fixation already partially inhibiting full expression of nitrogenase. High nitrogenase levels, about two-fold higher than those in normal N2-fixing Anabaena sp. ATCC 33047, are found in cell suspensions which have been treated with the glutamine synthetase inhibitor l-methionine-d,l-sulfoximine or subjected to nitrogen starvation. Filaments treated in either way are insensitive to the ammonia-promoted inhibition of nitrogenase development, although this insensitivity is only transitory for the nitrogen-starved filaments, which become ammonia-sensitive once they regain their normal nitrogen status.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 41
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 141 (1985), S. 244-248 
    ISSN: 1432-072X
    Schlagwort(e): Ammonia analogues ; Anabaena variabilis ; Cyanobacteria ; Ethylenediamine ; Nitrogen fixation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The cyanobacterium Anabaena variabilis showed a pH dependent uptake of ethylenediamine. No uptake of ethylenediamine was detected at pH 7.0. At higher pH values (e.g. pH 8.0 and pH 9.0) accumulation did occur and was attributed to diffusion of uncharged ethylenediamine in response to a pH gradient. A biphasic pattern of uptake was observed at these higher pH values. Treatment with l-methionine-d,l-sulphoximine (MSX) to inactivate glutamine synthetase (GS) inhibited the second slower phase of uptake without any significant alteration of the initial uptake. Therefore for sustained uptake, metabolism of ethylenediamine via GS was required. NH 4 + did not alter the uptake of ethylenediamine. Ethylenediamine was converted in the second phase of uptake to an analogue of glutamine which could not be detected in uptake experiments at pH 7.0 or in uptake experiments at pH 9.0 following pretreatment of cells with MSX. Ethylenediamine treatment inhibited nitrogenase activity and this inhibition was greatest at high pH values.
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  • 42
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 141 (1985), S. 364-370 
    ISSN: 1432-072X
    Schlagwort(e): Nitrogen fixation ; Denitrification ; Associative symbiosis ; Acetylene reduction ; Nitrous oxide formation ; Azospirillum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A model system is described where Azospirillum and germinated wheat seeds were grown in association for a week and then assayed for nitrogen fixation (C2H2-reduction) and denitrification (N2O-formation) activities. The association performed C2H2-reduction and N2O-formation under microaerobic conditions. Both activities were measurable after already 3–5 h of incubation with substantial rates and were strictly dependent on the presence of both plants and bacteria. During the week of the growth of the association, the bacteria had lived exclusively from the carbon compounds supplied by the roots of the plants. C2H2-reduction activity by the association was more or less the same with all the Azospirillum brasilense strains, but lower with A. lipoferum and with the A. amazonense strains tested. Two nitrogenase negative mutants of Azospirillum brasilense showed virtually no activity in the association. C2H2-reduction activity was strongly dependent on the growth temperature of the association. Denitrification (N2O-formation) was high also at higher temperatures and at pH-values in the medium around 7.8 but not at neutrality and was strictly dependent on nitrate. The Azospirillum strain used strongly determined the rate of the N2O-formation in the association. It is suggested that Azospirillum may be beneficial to crops particularly under tropical conditions.
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  • 43
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 23 (1985), S. 705-728 
    ISSN: 1573-4927
    Schlagwort(e): glucosephosphate isomerase (EC 5.3.1.9) ; Mytilus edulis ; alleloenzymes ; enzyme kinetics ; temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Two glucosephosphate isomerase (GPI;D-glucose-6-phosphate ketolisomerase; EC 5.3.1.9) alleloenzymes from the blue mussel,Mytilus edulis, were purified to homogeneity. The steady-state kinetic properties of GPI1.00 and GPI.96, which exhibit latitudinal clines in frequency along the Atlantic coast of North America, were determined in both the glycolytic and the gluconeogenic reaction directions at physiological temperatures and pH levels. The two alleloenzymes are catalytically similar at low temperatures (5–10°C), while GPI1.00 diverges to become more efficient at higher physiological temperatures (15–25°C). This pattern of differentiation is consistent with the latitudinal distributions of the alleloenzymes and is due to the greater temperature sensitivities of GPI1.00 V max /K m values of the two alleloenzymes are virtually the same over the physiological range of temperatures. The observed pattern of catalytic differentiation is similar to that seen for interspecific GPI variants.
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  • 44
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 66 (1985), S. 111-116 
    ISSN: 1573-4919
    Schlagwort(e): Adenosine triphosphatase ; Na+ ; K+ ; catecholamines ; calcium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary The interaction of noradrenaline, various cation chelators and calcium on Na+, K+-ATPase from rat cerebral cortex plasma membranes was studied. It was shown that chelation of inhibitory cations by EGTA, EDTA and dipyridyl activated Na+, K+-ATPase to the same extent as noradrenaline but at higher concentrations; increasing concentrations of EGTA depressed the activation by noradrenaline; calcium in the form of a calcium-EGTA buffer depressed Na+, K+-ATPase at physiological concentrations; the inhibition of Na+, K+-ATPase by calcium is dependent on the magnesium concentration in the assay and the inhibition by calcium was partially reversed by noradrenaline.
    Materialart: Digitale Medien
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  • 45
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 66 (1985), S. 145-149 
    ISSN: 1573-4919
    Schlagwort(e): calcium ; cyclic GMP ; gonadotropin releasing hormone ; guanylate cyclase ; manganese
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary Gonadotropin releasing hormone enhanced guanylate cyclase [E.C.4.6.1.2] two- to threefold in pituitary, testis, liver and kidney. Dose response relationships revealed that at a concentration of 1 nanomolar, gonadotropin releasing hormone caused a maximal augmentation of guanylate cyclase activity and that increasing its concentration to the millimolar range caused no further enhancement of this enzyme. There was an absolute cation requirement for gonadotropin releasing hormone's enhancement of guanylate cyclase activity as there was no increase without any cation present. Gonadotropin releasing hormone could increase guanylate cyclase activity with either calcium or manganese in the incubation medium but more augmentation was observed with manganese. The data in this investigation suggest that guanylate cyclase may play a role in the mechanism of action of gonadotropin releasing hormone.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 46
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 23 (1985), S. 705-728 
    ISSN: 1573-4927
    Schlagwort(e): glucosephosphate isomerase (EC 5.3.1.9) ; Mytilus edulis ; alleloenzymes ; enzyme kinetics ; temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Two glucosephosphate isomerase (GPI;D-glucose-6-phosphate ketolisomerase; EC 5.3.1.9) alleloenzymes from the blue mussel,Mytilus edulis, were purified to homogeneity. The steady-state kinetic properties of GPI1.00 and GPI.96, which exhibit latitudinal clines in frequency along the Atlantic coast of North America, were determined in both the glycolytic and the gluconeogenic reaction directions at physiological temperatures and pH levels. The two alleloenzymes are catalytically similar at low temperatures (5–10°C), while GPI1.00 diverges to become more efficient at higher physiological temperatures (15–25°C). This pattern of differentiation is consistent with the latitudinal distributions of the alleloenzymes and is due to the greater temperature sensitivities of GPI1.00 V max /K m values of the two alleloenzymes are virtually the same over the physiological range of temperatures. The observed pattern of catalytic differentiation is similar to that seen for interspecific GPI variants.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 47
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 67 (1985), S. 145-150 
    ISSN: 1573-4919
    Schlagwort(e): calcium ; harmaline ; smooth muscle ; sodium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary The effect of changing the extracellular concentration of both Na+ and K+ on the longitudinal muscle of the guinea-pig ileum was studied in the presence and absence of harmaline. A decrease in extracellular Na+ concentration was found to produce a dose-dependent contractile response, which may suggest the existence of a Na...Ca exchange mechanism in this muscle. Harmaline (2 × 10−4 M) was found to reversibly inhibit this contraction and was also found to selectively block the tonic component of high-K induced contradictions. In view of the fact that harmaline is a non-competitive inhibitor of Ca-induced contractions (Hider et al., Europ. J. Pharmacol., 71, 87, 1981), the action of harmaline was interpreted as being a specific inhibitor of the Na... exchange mechanism, binding specifically to Na+ coordination sites.
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  • 48
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 68 (1985), S. 115-120 
    ISSN: 1573-4919
    Schlagwort(e): pyruvate kinase isoenzymes ; pancreatic islets ; kinetic and immunological studies ; calcium ; alanine ; phenylalanine ; fructose bisphosphate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract To determine which of the major isoenzymes of pyruvate kinase pancreatic islet pyruvate kinase most resembled, it was compared to pyruvate kinase from other tissues in kinetic and immunologic studies. The pattern of activation by fructose bisphosphate and the patterns of inhibition by alanine and phenylalanine were most similar to those of the M2 isoenzyme from kidney and were dissimilar to those of the isoenzymes from skeletal muscle (type M1) and liver (type L). The islet pyruvate kinase was inhibited by anti-M1 pyruvate kinase serum (which crossreacts with the M2 isoenzyme), but not by anti-L pyruvate kinase. These results are most consistent with islets possessing predominantly, if not exclusively, the M2 isoenzyme of pyruvate kinase. We previously showed that rat pancreatic islet cytosol contains protein kinases that can catalyze a calcium-activated phosphorylation of an endogenous peptide that has properties, such as subunit molecular weight and isoelectric pH, that are identical to those of the M2 and M, isoenzymes of pyruvate kinase, and that islet cytosol can catalyze phosphorylation of muscle pyruvate kinase. In the present study it was shown that incubating islet cytosol with ATP under conditions known to permit phosphorylation and inhibition of liver pyruvate kinase did not affect the islet pyruvate kinase activity. It is concluded that phosphorylation of the islet pyruvate kinase has no immediate effect on enzyme activity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 49
    ISSN: 1432-2048
    Schlagwort(e): Ammonia/ammonium (assimilation, excretion) ; Anthoceros ; Bryophyta ; Cyanobacteria ; Nitrogen fixation ; Nostoc ; Symbiosis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The initial product of fixation of [13N]N2 by pure cultures of the reconstituted symbiotic association between Anthoceros punctatus L. and Nostoc sp. strain ac 7801 was ammonium; it accounted for 75% of the total radioactivity recovered in methanolic extracts after 0.5 min and 14% after 10 min of incubation. Glutamine and glutamate were the primary organic products synthesized from [13N]N2 after incubation times of 0.5–10 min. The kinetics of labeling of these two amino acids were characteristic of a precursor (glutamine) and product (glutamate) relationship. Results of inhibition experiments with methionine sulfoximine (MSX) and diazo-oxonorleucine were also consistent with the assimilation of N2-derived NH 4 + by Anthoceros-Nostoc through the sequential activities of glutamine synthetase (EC 6.3.1.2) and glutamate synthase (EC 1.4.7.1), with little or no assimilation by glutamate dehydrogenase (EC 1.3.1.3). Isolated symbiotic Nostoc assimilated exogenous 13NH 4 + into glutamine and glutamate and their formation was inhibited by MSX, indicating operation of the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway: However, relative to free-living cultures, isolated symbiotic Nostoc assimilated 80% less exogenous ammonium into glutamine and glutamate, implying that symbiotic Nostoc could assimilate only a fraction of N2-derived NH 4 + . This implication was tested by using Anthoceros associations reconstituted with wild-type or MSX-resistant strains of Nostoc incubated with [13N]N2 in the presence of MSX. The results of these experiments indicated that, in situ, symbiotic Nostoc assimilated about 10% of the N2-derived NH 4 + and that NH 4 + was made available to Anthoceros tissue where it was apparently assimilated by the GS-GOGAT pathway. Since less than 1% of the fixed N2 was lost to the suspension medium, it appears that transfer of NH 4 + from symbiont to host tissue was very efficient in this extracellular symbiotic association.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 50
    Digitale Medien
    Digitale Medien
    Springer
    The journal of membrane biology 86 (1985), S. 113-125 
    ISSN: 1432-1424
    Schlagwort(e): epithelial monolayers ; MDCK cells ; tight junctions ; calcium ; biosynthesis of junctions ; junctional assembly ; apical/basolateral polarization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary Synthesis and assembly of tight junctions are studied in monolayers of MDCK cells plated at a density sufficient for confluence, allowed to attach for 1 hr, and transferred to fresh media without cells containing or not Ca2+, 20 hr later, while monolayers with Ca2+ have fully developed junctions that confer an electrical resistance across of 346±51 Ω cm2, those without Ca2+ have a negligible resistance. If at this time Ca2+ is added, junctions assemble and seal with a fast kinetics, that can be followed through the development of electrical resistance, penetration of ruthenium red, and electron microscopy. Drugs that impair synthesis, maturation and transport of proteins (cycloheximide, tunicamycin, monensin) indicate that protein components are synthesized early upon plating, do not seem to require N-glycosylation, and are stored in the Golgi compartment. Upon addition of Ca2+ they are transferred to the membrane with the participation of microfilaments but not of microtubules. These components seem to insert directly in the position they occupy in the strands, and the cell circles its perimeter with one strand as early as 15 min, even if in some segments it only consists of a row of particles. New strands develop in association with previous ones, and the pattern completes in 4 to 6 hr. Ca2+ is required for the maintenance of the assembly and also for the sealing with neighboring cells. These processes cannot occur below 25°C. Serum is not required. Polarized distribution of intramembrane particles (IMP) in apical and basolateral regions follows the same time course as junction formation, in spite of the fence constituted by those strands that are already assembled. This suggests that IMP do not redistribute by lateral displacements in the plane of the membrane, but by removal and insertion in the apical and basolateral domains.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 51
    Digitale Medien
    Digitale Medien
    Springer
    The journal of membrane biology 85 (1985), S. 269-280 
    ISSN: 1432-1424
    Schlagwort(e): cell fusion ; electrofusion ; dielectrophoresis ; calcium ; magnesium ; membrane lipid ; lymphoma cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary Mouse leukemic lymphoblasts (L5178Y) brought into close contact by dielectrophoresis underwent cell fusion following the application of electrical pulses in the presence of electrolytes. The electrically fused cells became spherical after switching off the dielectrophoretic field. Fusion between a cell vitally stained with Janus Green and that with Neutral Red resulted in the homokaryon with a mixed color. Intracellular potentials simultaneously recorded from the two cells located on both sides of the homokaryon were identical. The fusion efficiency was remarkably dependent upon temperature, displaying a discontinuity at about 11°C in the Arrhenius plot. The extracellular application of phospholipase-A2 or-C suppressed the fusion yield. Thus, it appears that the phospholipid domains play a crucial role in the electric pulse-induced cell fusion. Treatment of the cells with proteolytic enzymes markedly enhanced the fusion yield, presumably due to removing the glycocalix and/or giving rise to fusion-potent, protein-free lipid domains. The presence of millimolar concentrations of divalent cations (irrespective of Mg2+ or Ca2+) as well as of micromolar concentrations of Ca2+ (but not Mg2+) was prerequisite to the resealing of membranes suffered from electrical breakdown upon exposure to electric pulses. In addition, extracellular Ca2+ (but not Mg2+) ions at more than micromolar concentrations were indispensable for the cell fusion.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 52
    Digitale Medien
    Digitale Medien
    Springer
    The journal of membrane biology 86 (1985), S. 9-15 
    ISSN: 1432-1424
    Schlagwort(e): molluscan neurone ; patch voltage-clamp technique ; single Cl channel ; calcium ; potassium ; multiplicity of the condutance states
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary Properties of the single Cl− channels were studied in excised patches of surface membrane from molluscan neurones using single-channel recording technique. These channels are controlled by Ca2+ and K+ acting on cytoplasmic and outer membrane surfaces, respectively, and by the membrane potential. The channels display about 16 intermediate conductance sublevels, each of them being multiples of ∼12.5 pS. The upper level of the channel conductance is about 200 pS. The channel behavior is consistent with an aggregation of channel-forming subunits into a cluster.
    Materialart: Digitale Medien
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  • 53
    Digitale Medien
    Digitale Medien
    Springer
    The journal of membrane biology 83 (1985), S. 147-156 
    ISSN: 1432-1424
    Schlagwort(e): Exocytosis ; proton pump ; calcium ; secretion ; adrenal medulla
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary Calcium-dependent exocytosis in ‘leaky’ bovine adrenal medullary cells has a requirement for Mg-ATP. One possibility is that exocytosis depends in some way on the operation of the ATP-dependent proton pump that serves to maintain the core of the secretory vesicles both acid and at a positive potential with respect to the cytosol. This possibility has been tested in ‘leaky’ cells by monitoring exocytosis under conditions where the secretory vesicle pH and potential gradients are measuredin situ. The results show rather clearly that exocytosis can persist, with unchanged Ca-activation kinetics, in the virtual absence both of a difference in pH between the cytosol and secretory vesicle core and also of a difference in potential across the vesicle membrane. The results do not, however, exclude a small modulating effect of vesicle pH or potential on exocytosis and shed no light on whether or not the plasma membrane potential, which is maintained close to zero in these experiments, influences exocytosis.
    Materialart: Digitale Medien
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  • 54
    Digitale Medien
    Digitale Medien
    Springer
    The journal of membrane biology 84 (1985), S. 207-219 
    ISSN: 1432-1424
    Schlagwort(e): sodium/calcium exchange ; excitation-contraction coupling ; sarcolemma ; membrane potential ; sodium ; calcium ; heart
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary The effect of membrane potential on sodium-dependent calcium uptake by vesicles in an isolated cardiac sarcolemma preparation was examined. Initial time course studies showed that the reaction deviated from initial velocity conditions within minutes. This appeared to be due, in part, to loss of the sodium gradient. Assays carried out to 10 sec revealed a linear component of uptake (2 to 10 sec) and a faster component (complete by 2 sec). The latter was eliminated by loading the preparation with ethyleneglycol-bis-(β-aminoethyl ether)N,N′-tetraacetic acid (EGTA). This maneuver did not affect the slow component, and subsequent studies used preparations containing EGTA. Potassium Nernst potentials (E K), established by potassium gradients in the presence of valinomycin, were varied from −100 to +30 mV by changing [K+] o from 1.18 to 153.7mM ([K+] i =50mM). The initial velocity of sodium-dependent calcium uptake was stimulated twofold by changingE K from −100 to 0 mV and another twofold by raisingE K from 0 to +30 mV. For the total range ofE K and [K+] o , 32 to 36% of the increase appeared to reflect stimulation by extravesicular potassium. The remainder appeared to be due to membrane potential. The profile of sodium-dependent calcium uptake versusE K suggested that calcium influx through electrogenic sodium/calcium exchange may be much more affected by the positive region of the cardiac action potential than by the negative region.
    Materialart: Digitale Medien
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  • 55
    ISSN: 1432-1424
    Schlagwort(e): calcium ; rod photoreceptors ; surface potentials
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary The nature of the Ca2+ buffer sites in intact rod outer segments isolated from bovine retinas (ROS) was investigated. The predominant Ca2+ buffer in intact ROS was found to be negatively charged groups confined to the surface of the disk membranes. Accordingly, Ca2+ buffering in ROS was strongly influenced by the electrostatic surface potential. The concentration of Ca2+ buffer sites was about 30mm, 80% of which were located at the membrane surface in the intradiskal space. A comparison with observations in model systems suggests that phosphatidylserine is the major Ca2+ buffer site in ROS. Protons and alkali cations could replace Ca2+ as mobile counterions for the fixed negatively charged groups. At physiological ionic strength, the total number of these diffusible, but osmotically inactive, counterions was as large as the number of osmotically active cations in ROS. The surface potential is dependent on the concentration of cations in ROS and can be measured with the optical dye neutral red. Addition of cations to the external solution led to the release of the internally bound dye as the cations crossed the outer membrane. The chemical and spectral properties of the dye enable its use as a real-time indicator of cation transport across the outer envelope of small particles in suspension. In this study, the dye method is illustrated by the use of well-defined ionophores in intact ROS and in liposomes. In the companion paper this method is used to describe the cation permeabilities native to ROS.
    Materialart: Digitale Medien
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  • 56
    ISSN: 1432-1424
    Schlagwort(e): surface charge ; potassium channel ; calcium ; phosphatidylserine ; planar bilayer
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary A Ca-activated, K-selective channel from plasma membrane of rat skeletal muscle was studied in artificial lipid bilayers formed from either phosphatidylethanolamine (PE) or phosphatidylserine (PS). In PE, the single-channel conductance exhibited a complex dependence on symmetrical K+ concentration that could not be described by simple Michaelis-Menten saturation. At low K+ concentrations the channel conductance was higher in PS membranes, but approached the same conductance observed in PE above 0.4m KCl. At the same Ca2+ concentration and voltage, the probability of channel opening was significantly greater in PS than PE. The differences in the conduction and gating, observed in the two lipids, can be explained by the negative surface charge of PS compared to the neutral PE membrane. Model calculations of the expected concentrations of K+ and Ca2+ at various distances from a PS membrane surface, using Gouy-Chapman-Stern theory, suggest that the K+-conduction and Ca2+-activation sites sense a similar fraction of the surface potential, equivalent to the local electrostatic potential at a distance of 9 Å from the surface.
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  • 57
    ISSN: 1432-0878
    Schlagwort(e): Smooth muscle cells ; Phenotype ; Electron microscopy ; DNA synthesis ; Man
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Smooth muscle cells were isolated enzymatically from adult human arteries, grown in primary culture in medium containing 10% whole blood serum, and studied by transmission electron microscopy and [3H]thymidine autoradiography. In the intact arterial wall and directly after isolation, each smooth muscle cell had a nucleus with a wide peripheral zone of condensed chromatin and a cytoplasm dominated by myofilament bundles with associated dense bodies. After 1–2 days of culture, the cells had attached to the substrate and started to spread out. At the same time, a characteristic fine-structural modification took place. It included nuclear enlargement, dispersion of the chromatin and formation of large nucleoli. Moreover, myofilament bundles disappeared and an extensive rough endoplasmic reticulum and a large Golgi complex were organized in the cytoplasm. This morphological transformation of the cells was completed in 3–4 days. It was accompanied by initiation of DNA replication and mitosis. The observations demonstrate that adult human arterial smooth muscle cells, when cultivated in vitro, pass through a phenotypic modulation of the same type as arterial smooth muscle cells from experimental animals. This modulation gives the cells morphological and functional properties resembling those of the modified smooth muscle cells found in fibroproliferative lesions of atherosclerosis. Further studies of the regulation of smooth muscle phenotype and growth may provide important clues for a better understanding of the pathogenesis of atherosclerosis.
    Materialart: Digitale Medien
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  • 58
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 239 (1985), S. 219-228 
    ISSN: 1432-0878
    Schlagwort(e): Neuromuscular junctions ; Nervous system ; Electron microscopy ; Sea-urchin ; Echinodermata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The fine structure of the soft tissues at the bases of spines in the sea-urchin Echinus esculentus has been examined with particular reference to the innervation of these appendages. The basal nerve ring encircling the spine contains many somata of neurones, and circumferentially directed elements, as well as tangled neuropil. The smooth muscles that bring about spine-pointing movements are innervated by terminals that contain two different types of vesicles, suggesting dual innervation by neurones containing different neurotransmitters. The neuromuscular junctions include apparent synapses between nerve cell bodies and muscle fibres. There are also neural elements that may be involved in the control of the catch apparatus of the spine. The complexity of the nerve ring and effector innervation implies that coordination of spine movements is more sophisticated than has been previously supposed.
    Materialart: Digitale Medien
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  • 59
    ISSN: 1432-0878
    Schlagwort(e): Antigen transfer ; Electron microscopy ; Enterocytes ; Macrophages ; Fish
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Two protein antigens, horseradish peroxidase (HRP) and ferritin, have been administered to the digestive tract of carp. Electron-microscopical observations reveal considerable absorption of both antigens in the second segment of the gut (from 70 to 95% of the total length) and also, although to a lesser extent, in the first segment (from 0 to 70% of the total length). Even when administered physiologically with food, a large amount of ferritin is absorbed by enterocytes in the second gut segment. HRP and ferritin are processed by enterocytes in different ways. HRP seems to adhere to the apical cell membrane, probably by binding to receptors, and is transported in vesicles to branched endings of lamellar infoldings of the lateral and basal cell membrane. Consequently, most of the HRP is released in the intercellular space where it contacts intra-epithelial lymphoid cells. Only small amounts of HRP become localized in secondary lysosomes of enterocytes. Ferritin does not bind to the apical cell membrane; after uptake by pinocytosis, it is present in small vesicles or vacuoles that appear to fuse with lysosome-like-bodies. In the second segment, intact ferritin ends up in the large supranuclear vacuoles (after 8 h), where it is digested slowly. Although no ferritin is found in the intercellular space, ferritin-containing macrophages are present between the epithelial cells, in the lamina propria and also to a small extent in the spleen. The transport of antigens from the intestinal lumen, through enterocytes, to intra-epithelial lymphoid cells or macrophages may have immunological implications, such as induction of a local immune response and prospectives for oral vaccination.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 60
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 239 (1985), S. 235-239 
    ISSN: 1432-0878
    Schlagwort(e): Erythropoiesis ; Autophagy ; Mitochondria ; Electron microscopy ; Stereology ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Late erythroblasts and reticulocytes from bone marrow of male Wistar rats were studied by electron-microscopic stereology. Late erythroblasts with morphological signs of nuclear extrusion (EN+erythroblasts) and late erythroblasts without these signs (EN-erythroblasts) were analysed separately. The volumes of mitochondria, autophagosomes, autophagocytosed mitochondria, autophagocytosed cytoplasm and degraded material inside autophagosomes were calculated per unit volume of cytoplasm. The results demonstrate that (1) the volume density of mitochondria in the cytoplasm decreases by 34% during maturation from (EN-)- to (EN+)-erythroblasts (p〈 0.001) and by 60% during differentiation from (EN+)-erythroblasts to reticulocytes (p〈0.001), (2) a fivefold increase in the volume density of autophagosomes in the cytoplasm is noted during maturation from (EN-)- to (EN+)-erythroblasts (p〈0.01), whereas the value of this parameter remains essentially unchanged during the subsequent differentiation to reticulocytes, (3) no mitochondria are found inside autophagosomes of (EN-)-erythroblasts, whereas mitochondria occupy 26% and 35%, respectively, of the autophagosomal volume in (EN+)-erythroblasts and in reticulocytes. Our results show that autophagocytosis of mitochondria starts at the moment of nuclear extrusion and continues in the bone marrow reticulocytes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 61
    ISSN: 1432-0878
    Schlagwort(e): Parietal cells ; Human stomach ; Peanut lectin ; Carbohydrate histochemistry ; Electron microscopy ; Man
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Peanut lectin reactivity was examined in normal fundic glands from human gastric samples, both at light- and electron-microscopic levels, using a peroxidase conjugate. Positive reaction was observed in the glycocalyx of parietal cell secretory canaliculi as well as in the mucous globules of mucous cells and in the luminal cell coat of chief cells. The presence of terminal galactose in the canalicular glycocalyx may be connected with the peculiar function of hydrochloric acid secretion. Peroxidase-labelled peanut lectin is proposed as a marker for visualizing the secretory canaliculus of parietal cells.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 62
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 239 (1985), S. 467-476 
    ISSN: 1432-0878
    Schlagwort(e): Endometrium, rat ; Stromal cells ; Hemosiderin ; Histochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Cytoplasmic pigment inclusions of rat endometrial stromal cells were studied by histology, histochemistry, fluorescence microscopy, electron microscopy and X-ray microprobe analysis. It is shown that a number of endometrial perivascular stromal cells contain numerous free cytoplasmic ferritin particles as well as hemosiderin vacuoles. The larger pigment inclusions reveal also positive PAS- and Schmorl reactions indicating that they contain polysaccharide and lipofuscin material, respectively. These pigmentstoring stromal cells also display acid phosphatase activity; they avidly phagocytose instillated latex particles. No pigment-storing cells occur within the surface or glandular epithelium, either in the basal endometrium or in the myometrium. It is demonstrated that the endometrial iron-storing cells function as iron depots; they take part in the phagocytosis and endocytosis of extracellular tissue components and therefore can be named phagocytes. Our data show that “fibroblastoid” endometrial stromal cells may differentiate into endometrial resident phagocytes, which ensure interstitial proteolysis and hence facilitate the drainage of extracellular fluid into the venous blood capillaries.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 63
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 239 (1985), S. 547-553 
    ISSN: 1432-0878
    Schlagwort(e): Endochondral bone development ; Osteoblast ; Osteoclast ; Horseradish peroxidase ; Electron microscopy ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary To investigate the mechanisms whereby bone cells absorb organic bone-matrix components during endochondral bone development, rat humeri were examined, employing horseradish peroxidase as a soluble protein tracer. Intravenously-injected peroxidase filled the osteoid layer and penetrated into the osteocyte lacunae and canaliculi, but did not enter the mineralized bone matrix. Whereas osteocytes rarely took up exogenous peroxidase, osteoblasts and osteoclasts actively endocytosed peroxidase in pinocytotic coated vesicles, tubular structures, and vacuoles. They also formed endocytotic vacuoles containing peroxidase in the Golgi area. The Golgi apparatus and dense bodies of these bone cells were, however, free of reaction products. Osteoclast ruffled borders were responsible for peroxidase absorption. In the osteoblast, osteocyte and osteoclast, endogenous peroxidatic reaction was detected only in mitochondria and not in other membrane-bounded vesicles and bodies. These results strongly suggest that both osteoblasts and osteoclasts participate in the resorption of bone-matrix organic components during bone remodelling.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 64
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 240 (1985), S. 199-208 
    ISSN: 1432-0878
    Schlagwort(e): Ovary ; Postovulatory follicles ; Electron microscopy ; Enzyme histochemistry ; Teleosts, catfish
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In captive African catfish, Clarias gariepinus, ovulation was induced with human chorionic gonadotropin (HCG) 4 I.U./g body weight to study the function of postovulatory follicles (POFs). Ultrastructural and enzyme-histochemical data indicate that, apart from special theca cells, the granulosa of relative young POFs (i.e., from 16 h and 28 h after HCG-injection) is capable of producing steroids. Possible functions of the synthesized steroids are discussed. Histological comparison of POFs from stripped and from unstripped fish, as well as histochemical investigation of the contents of ovulated ova and granulosa of POFs at 48 h after HCG-injection, showed that the latter structure is involved in phagocytosis of the disintegrating ovulated eggs. The polysaccharide-lipid-protein material, initially taken up by heterophagolysosomes of the granulosa cells, subsequently undergoes fatty degeneration. The granulosa cells of the POFs showed strong acid phosphatase activity and abundant granular endoplasmic reticulum from 16 h after HCG-injection onward; heterophagolysosomes appeared at 32 h. These results indicate that after ovulation the phagocytotic function of the granulosa develops progressively. Autophagolysosomes, responsible for the final disintegration of POFs, become increasingly evident in the granulosa cells with increasing time after ovulation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 65
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 240 (1985), S. 243-246 
    ISSN: 1432-0878
    Schlagwort(e): Liver ; Hepatectomy ; Hepatocyte regeneration ; Endoplasmic reticulum ; Electron microscopy ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Light-microscopic analysis of mouse liver homogenates six days after partial hepatectomy, showed a higher percentage of nuclei with adherent cytoplasm than homogenates from normal liver. This observation was true for animals with either a slow or rapid recovery of body weight after the operation. The phenomenon was not a function of the changes in the proportions of parenchymal and non-parenchymal tissue in the regenerating liver. Electron-microscopic analysis of random samples from normal and regenerating livers indicated an increase in the perinuclear rough endoplasmic reticulum, and a displacefment of the glycogen depots within the regenerating cells six days after partial hepatectomy. The marked resistance towards homogenization, shown by the cytoplasm of the regenerating cells, may have been due to the observed increase of perinuclear membranes. However, qualitative changes of the cell membranes and a general decrease of proteolytic activity connected with liver regeneration may also have contributed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 66
    ISSN: 1432-0878
    Schlagwort(e): Adrenergic nerves ; Acetylcholinesterase ; containing nerves ; Ovary ; Electron microscopy ; 5-Hydroxydopamine ; Guinea-pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The autonomic nerve supply of the guinea-pig ovary was investigated by a combination of light-and electron microscopy. At the light-microscopic level, adrenergic fibres were identified due to their formaldehyde-induced fluorescence. In addition, the ovary contained acetylcholinesterase-positive fibres. In all parts of the ovary, the adrenergic fibres were most numerous. At the ultrastructural level it was possible to identify the adrenergic nerve terminals with the aid of the false adrenergic transmitter, 5-hydroxydopamine. Thus, large numbers of adrenergic terminals, characterized by their content of 50–60 nm, electron-dense synaptic vesicles, were seen within the interstitial gland, where they formed close contacts with the endocrine cells (membrane-to-membrane distance, 20–100 nm). The follicular theca externa was also richly supplied by adrenergic nerves. At this location, close contacts (50–100 nm) were identified between the nerve terminals and the smooth muscle-like cells. Very few adrenergic nerve fibres were present in the theca interna of follicles or in the corpus luteum. Non-adrenergic nerve terminals, characterized by electronlucent synaptic vesicles of 50–60 nm diameter, were observed together with the adrenergic fibres. They were always present in much lower numbers than the latter. No “p-type” nerves were identified by electron microscopy.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 67
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 242 (1985), S. 445-448 
    ISSN: 1432-0878
    Schlagwort(e): Extrahypothalamic neurosecretory neurons ; Teleost mesencephalon ; Brain stem nuclei ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Neurons in the dorsal tegmentum of the midbrain of the teleosts Poecilia sphenops and P. latipinna were examined by use of electron microscopy. A nucleus of neurosecretory neurons was identified in the subependymal region just dorsal to the medial longitudinal fascicle. This nucleus has been called the dorsal tegmental magnocellular nucleus (DTMN). The most distinguishing cytological feature of these cells is the presence of large granular vesicles, 100–180 nm in diameter. These vesicles resemble neurosecretory granules characteristically found in preoptic and lateral tuberal magnocellular neurosecretory cells. Presynaptic terminals on these cells contain small clear vesicles, and some among them contain small dense-core vesicles.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 68
    ISSN: 1432-0878
    Schlagwort(e): Vocal motor system ; Songbirds (zebra finch) ; Calcium-binding proteins ; Parvalbumin ; Electron microscopy ; Plasticity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of parvalbumin (PV) within neurons of the vocal motor nucleus hyperstriatum ventralepars caudalis (HVc) was investigated in the forebrain of adult male zebra finches by means of light and electron microscopy using the indirect immunoperoxidase technique. Parvalbumin-reaction product was located in the amorphous material of perikarya, dendrites and nuclei, and associated to microtubuli, postsynaptic densities and intracellular membranes; it was found in some axons and Gray type-2 boutons, but rarely in type-1 boutons and never in the Golgi apparatus. These observations suggest that parvalbumin may regulate calcium-dependent processes at the postsynaptic membrane and in the cytosol. Furthermore, the partial association of parvalbumin to microtubuli points to an involvement in calcium-dependent tubular functions. Calcium currents and microtubular assembly or transport may be relevant for the known functions of HVc in song learning.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 69
    ISSN: 1432-0878
    Schlagwort(e): CRF immunohistology ; Paraventricular nuclei ; Pituitary gland, pars nervosa ; Electron microscopy ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A fine network of corticotropin-releasing factor (CRF)-immunopositive fibers was found in the posterior lobe of the pituitary of the rat. The intermediate and distal lobes were free of CRF-immunoreactivity. Varicose, terminal-like axons were frequently observed around capillary vessels. Surgical isolation of the paraventricular nuclei resulted in a complete disappearance of CRF-immunoreactive fibers from the posterior lobe. CRF-immunopositive fibers show the general characteristics of peptidergic axons. These ultrastructural observations support the idea that CRF is secreted into capillary vessels.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 70
    ISSN: 1432-0878
    Schlagwort(e): Thyroid follicle ; Suspension culture ; Cellular polarity ; Tight junctions ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Porcine thyroid follicles, when isolated by enzymatic digestion and suspended in Eagle's MEM containing 10% fetal calf serum, undergo inversion of cellular polarity. After isolation, the strands for the tight junctions (zonulae occludentes) between follicle cells begin to move towards the side of the medium and gather at this side of the lateral plasma membrane during 24 h of incubation. Around this time, microvilli of many follicular cells protrude into the culture-medium. The elements of the Golgi apparatus are located at the luminal as well as the culture-medium side of the cytoplasm, and also at the lateral side of the nucleus after 24 h of suspension culture, and by 94 h of incubation almost all elements of this organelle, as well as lysosomes and the central cilium have migrated to the side of the medium. The migration of the zonulae occludentes is considered to be the initial change in the reversal of the polarity of this cell.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 71
    ISSN: 1432-0878
    Schlagwort(e): Reissner's fiber ; Subcommissural organ ; Ependyma ; Immunocytochemistry ; Electron microscopy ; Bovine
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Reissner's fiber (RF) of the subcommissural organ (SCO), the central canal and its bordering structures, and the filum terminale were investigated in the bovine spinal cord by use of transmission electron microscopy, histochemical methods and light-microscopic immunocytochemistry. The primary antisera were raised against the bovine RF, or the SCO proper. Comparative immunocytochemical studies were also performed on the lumbo-sacral region of the rat, rabbit, dog and pig. At all levels of the bovine spinal cord, RF was strongly immunoreactive with both antisera. From cervical to upper sacral levels of the bovine spinal cord there was an increasing number of ependymal cells immunostainable with both antisera. The free surface of the central canal was covered by a layer of immunoreactive material. At sacral levels small subependymal immunoreactive cells were observed. From all these structures sharing the same immunoreactivity, only RF was stained by the paraldehyde-fuchsin and periodicacid-Schiff methods. At the ultrastructural level, ependymal cells with numerous protrusions extending into the central canal were seen in the lower lumbar segments, whereas cells displaying signs of secretory activity were principally found in the ependyma of the upper sacral levels. A few cerebrospinal fluid-contacting neurons were observed at all levels of the spinal cord; they were immunostained with an anti-tubulin serum. The lumbo-sacral segments of the dog, rat and rabbit, either fixed by vascular perfusion or in the same manner as the bovine material, did not show any immunoreactive structure other than RF. The possibilities that the immunoreactive ependymal cells might play a secretory or an absorptive role, or be the result of post-mortem events, are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 72
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 242 (1985), S. 245-256 
    ISSN: 1432-0878
    Schlagwort(e): Myelin ; Microglia ; Wallerian degeneration ; Optic nerve, rat ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Adult albino rats were subjected to unilateral surgical removal of the eyeball. After survival times of 7–140 days, the numerical response of the neuroglial cells, and the progressive disintegration of the myelin sheaths in the optic nerves, were studied qualitatively and quantitatively in electron-microscopic montages. The distribution density of microglia and astroglia in degenerating optic nerve increased to peaks after 35 and 56 days respectively, whereas, the oligodendroglia gradually decreased. During the early stage of degeneration, microglial cells appeared and invaded the sheath at the intraperiod line, peeling off the outer lamellae, which were then engulfed by phagocytosis. Within the microglia, myelin sheath fragments were surrounded by a membrane curled to form a myelin ring. In the intermediate stage of degeneration, the paired electrondense lines of the ring, made up of myelin basic protein, decomposed and formed a homogenous or heterogenous osmiophilic layered structure, the myelin body, which, in the final stages, disintegrated and transformed into globoid lipid droplets and needle shaped cholesterol crystals.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 73
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 242 (1985), S. 127-143 
    ISSN: 1432-0878
    Schlagwort(e): Paraphysis cerebri ; Histology ; Cytochemistry ; Electron microscopy ; Autoradiography ; Salmo gairdneri Richardson
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The paraphysis cerebri of adult Salmo gairdneri is represented by a differentiated part of the pars impar telencephali of the telencephalic roof. It consists of a vaulted epithelial sheet, which displays only a few rostral evaginations and separates the cerebrospinal fluid (CSF) from the meningeal interstitial fluid. The fenestrated, sinusoidal portal system surrounding the paraphyseal epithelium appears to be part of a complex vascular bed of the dorsal telencephalic and diencephalic area. Myelinated and unmyelinated nerve fibers observed in the vicinity of the paraphyseal epithelium fail to make synaptic contact with paraphyseal cells. The single-layered epithelium is composed of characteristic, rather small, optically dense, cuboidal and cylindrical cells, apically mutually attached by junctional complexes including zonulae occludentes. These paraphyseal cells execute a high energetic and a moderate synthetic metabolism as indicated by ultrastructural, cytochemical and enzyme-cytochemical observations. Morphological evidence is presented for a multiple function of these cells in the regulation of the CSF: 1) water and solute elaboration into the ventricular system, 2) restricted uptake of high molecular weight organic substances from the CSF, 3) restricted uptake of low molecular weight substances from the CSF, but apparently not of GABA and of biogenic amines, 4) the formation and pinching-off of “blebs” as expression of a physiological mechanism not yet elucidated. The possible relationship between the level of development of the paraphysis cerebri and the sensitivity of animals to hydro-mineral metabolism is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 74
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 169-175 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Poaceae =Gramineae ; Lolium ; Morphology ; protein electrophoresis ; numerical methods
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Morphological analysis and electrophoresis of seed proteins of fiveLolium species disclosed that they form two distinct groups corresponding to those recognized from compatibility data.Lolium temulentum andL. remotum of the self-pollinated group were shown to be distinct but closely related species. Morphological intergradation and high similarities between protein profiles ofL. perenne, L. multiflorum andL. rigidum (cross-pollinated species) suggest little genetic differentiation between these taxa. This implies that treatment at the infraspecific level might better accomodate the data.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 75
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 177-184 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Scrophulariaceae ; Scrophularieae ; Scrophularia himalensis ; Endosperm ; endosperm haustoria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Scrophularia himalensis has anab initio cellular endosperm. A transverse division separates a micropylar chamber from a chalazal chamber. The second division is vertical in both, the third is also vertical but at right angles to the second and restricted to the micropylar chamber just as the fourth transverse division. The four-celled micropylar haustorium is branched, highly aggressive, and persists for a long time during seed development. The bicelled chalazal haustorium is non-aggressive and is relatively short-lived. The endosperm proper is ruminate. Variation in the early ontogeny of the endosperm and the structure of endosperm haustoria in the tribeScrophularieae are evaluated.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 76
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 239-246 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Poaceae (Gramineae) ; Bambusoideae ; Bambusa ; Dendrocalamus ; Pseudostachyum ; Melocalamus ; Melocanna ; Ochlandra ; Embryo
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The mature embryo of seven species belonging to five genera of Indian bamboos is described. In all these the basic pattern of embryo organisation is same: the scutellar and coleoptilar bundles are not separated by an internode, the epiblast is absent, the lower portion of the scutellum and the coleorhiza are separated by a cleft and the margins of embryonic leaves overlap. The features unique to fleshy fruited bamboos are: presence of a massive scutellum, the juxtaposition of plumule and radicle and the occurrence of a bud in the axil of the coleoptile. The fleshy fruit bearing bamboos should be classified into one group, the tribeMelocanneae. Evidence is provided to recognise additional groups in the subfamilyBambusoideae.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 77
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 215-237 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Scrophulariaceae ; Rhinanthoideae ; Euphrasia ; E. hirtella ; E. drosocalyx ; Infraspecific classification ; chorology of the Alpine Flora ; Flora of Europe
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A more precise taxonomic concept ofE. hirtella and its infraspecific synonymy is presented. Its diploid nature (2n = 22) is confirmed. Within the European area ofE. hirtella five different races may be recognised: “typical”, “brandisii”, “capitulata”, “Rofan” and “Bretagne”. Taxonomic rank is not yet attributed to these races. The heterogeneous taxonomic assembly “E. drosocalyx” is disentangled. The type refers to products of hybrid introgression ofE. rostkoviana-characters (long glandular hairs) intoE. minima.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 78
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 247-252 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Typhaceae ; Sparganiaceae ; Typha ; Esterases ; ADH ; GDH disc electrophoresis ; speciation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The enzymatic spectra ofTypha angustifolia, T. latifolia and their hybrids were examined by disc electrophoresis for three enzymatic systems: esterases, alcohol deshydrogenase, and glutamate deshydrogenase. The intermediate position of the hybrids is confirmed. The study of alcohol deshydrogenase reveales the existence of an intraspecific variability inT. angustifolia between individuals from the Massif Armoricain in France and Dellys in Algeria.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 79
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 287-289 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Cactaceae ; Neolloydia intertexta ; Self-compatability ; pollination ecology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Self and outcross pollination treatments were applied toNeolloydia intertexta. Two distinct patterns of seed production were found, and are correlated with self-pollinated outcrossed treatments. The outcrossed result resembles the field exposed crop. The untouched controls produce a similar pattern to the actively selfed group. The self treatments produce substantially less seed than the outcrosses; thus, self-compatability is incomplete.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 80
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 291-312 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Boraginaceae ; Microparacaryum gen. nov. ; Paracaryum ; Mattiastrum ; Taxonomy ; distribution maps ; heteromericarpy ; fruit polymorphism ; Flora of Western Asia (Turkey, U.S.S.R., Iran, Iraq, Afghanistan, Pakistan)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Microparacaryum (M. Pop. exH. Riedl)Hilger & Podlech is described as a new genus of theBoraginaceae-Cynoglosseae. It comprises the annual species hitherto included inParacaryum (DC.)Boiss. andMattiastrum (Boiss.)Brand. Distribution maps are given for all 3 genera.Microparacaryum consists of two species,M. salsum (Boiss.)Hilger & Podlech (M. s.) andM. intermedium (Fresen.)Hilger & Podlech (M. i.). ParticularlyM. i. is a very variable species, and most of the species formerly recognized belong here. Scattered all over the range of the genus, plants occur with nutlets exhibiting flat or incurved marginal wings, often in mixed populations. This fruit polymorphism is taxonomically treated by recognizing “formae”. In addition, the following new infraspecific taxa and combinations are described:M. i. var.intermedium formaparacaryoides Hilger & Podlech,M. i. var.stellatum (H. Riedl)Hilger & Podlech,M. i. var.stellatum formamattiastroides Hilger & Podlech,M. s. formamattiastroides Hilger & Podlech.
    Materialart: Digitale Medien
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  • 81
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 317-319 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Polygonaceae ; Rumex ; New species ; hybrids ; Turkey
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Rumex bithynicus sp. n. (sect.Rumex) with two new hybrids from NW Turkey is close toR. alveolatus (from NE Iran, Afghanistan, NW Pakistan and Turkmenistan).
    Materialart: Digitale Medien
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  • 82
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 148 (1985), S. 253-285 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Annonaceae ; “Fusaea subfamily” ; Anaxagorea ; Meiocarpidium ; Palynology ; pollen ultrastructure ; intine extrusion ; systematic and phylogenetic relationships
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract LM, SEM and TEM observations document similar palynological features for paleo- and neotropical species ofAnaxagorea. Pollen grains are solitary, heteropolar, ellipsoidal to globose, and broadly sulcate. The exine consists of a smooth and microperforated tectum, a granular to “protocolumellar” infratectal layer, and sometimes an initial, not foliated basal layer. The intine is stratified and tends to extrude with its considerably swelling alveolarfibrillar outer layer through the aperture, already inside the unopened pollen sacs. This appears as a new and irreversible harmomegathic mechanism, limited toAnaxagorea, Meiocarpidium and possibly otherAnnonaceae. These palynological data are discussed and coordinated with those from gross-morphological, anatomical, karyological, phytochemical and other studies. All this offers convincing proof for the sometimes questioned systematic and phylogenetic coherence of the widely disjunct S. & C. American and SE. Asian members ofAnaxagorea. Furthermore, a broad comparison with otherAnnonaceae genera demonstrates somewhat closer links ofAnaxagorea with several small and relictual African genera, i.e.Meiocarpidium andPolyceratocarpus, but alsoLettowianthus andPiptostigma;Cleistopholis andAmbavia are more isolated. These genera which exhibit many “primitive” features are provisionally arranged under “tribe I” of the so-called “Fusaea subfamily”. There are loose affinities with “tribe II”, an assembly of more advanced and widespread, often proliferating generic groups, including the S. AsianCananga, the pantropicalXylopia (withPseudannona from Mauritius), a possible link toAnaxagorea and “tribe I”, and the S. AsianGoniothalamus (with the AustralasianRichella s. str. and the W. AfricanNeostenanthera andBoutiquea); the neotropicalFusaea andDuckeanthus apparently are more remote. Because of relationships to several otherAnnonaceae groups and difficulties with its circumscription and definition, the maintainance of the “Fusaea subfamily” as a whole appears questionable.
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  • 83
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 1-18 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Scrophulariaceae ; Rhinanthoideae ; Euphrasia ; E. alpina ; E. christii ; E. cisalpina ; Chorology and history of the Alpine flora
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The taxonomic affinities, circumsciription and racial differentiation ofE. alpina and its infraspecific synonymy are considered. Its diploid nature is confirmed. The yellow-floweringE. christii is also diploid (2n = 22, first account). A detailed comparison withE. alpina (Figs. 1–2) suggests the rank of subspecies only, and gives no hints as to its suggested hybrid origin. The distribution of the two taxa is documented in maps (Figs. 3–5).
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  • 84
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 19-45 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Poaceae ; Festuca vivipara ; F. ovina subsp.supina ; Chromosome numbers ; external morphology ; anatomy ; ecology ; distribution ; phylogeny ; Flora of the Central and Eastern Alps ; of Austria ; Slovenia ; N. Italy ; Switzerland ; and Spain
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The differences betweenFestuca vivipara (tetraploid) andF. ovina subsp.supina (diploid), two often confused taxa, are demonstrated in regard to morphology, leaf anatomy, ecology and distribution. New maps illustrate the distribution ofF. vivipara in the Alps and the Northern Hemisphere. The development of different polyploidy levels withinF. vivipara as a consequence of occasional sexual processes is discussed. (English Summary on p. 39.)
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  • 85
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 65-69 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Linaceae ; Linum ; sect.Macrantholium ; Pollen
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Pollen of the two distylous species which make upLinum sect.Macrantholinum differs from that of other distylous species in the genus in being multiporate and in having much more modest differences in exine sculpturing between grains from long- and short-styled plants. Pollen morphology does not help in relating the two species to others in the genus but does support their retention in a separate section.
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  • 86
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 71-88 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Umbelliferae (Apiaceae) ; Bunium ; Chromosome numbers ; karyotypes ; karyosystematics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Chromosome numbers are reported for 23 species of the genusBunium, distributed mainly in Middle Asia and Transcaucausia. The great diversity of basic chromosome numbers is unusual for a genus ofUmbelliferae: x = 11, 10, 9, 8, 7, and 6. This series obviously has to be interpreted as descending dysploidy. Infraspecific aneuploidy was found in three species, but there are no B-chromosomes or polyploidy. The karyotypes of 18 species are described, using two morphometric chromosome parameters, centromere position and relative length (Table 2), and illustrated (Figs. 2–4). Species vary greatly in their karyotype asymmetry (20.4–45.9%). Chromosome numbers and structures appear to be useful in the karyotaxonomical and biogeographical analysis of the genus.
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  • 87
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 119-134 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Orchidaceae ; Androecium morphology ; pollination ; evolutionary trends
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The evolution of the androecium in theOrchidaceae shows three major trends. There is a progressive trend in the degree of fusion of the filament(s) and staminode(s) to the gynoecium. Secondly, there is a reduction in the number of fertile anthers. Finally, there is a progressive change in the position of the base of the anther relative to the apex of the stigma; in the more primitive orchids the apex of the stigma is always higher than the base of the anther (this position is reversed in the higher orchids). All three trends reflect variation in the evolution of pollen dispersal and pollen reception mechanisms in theOrchidaceae. Trends in the evolution of the orchid anther(s) tend to parallel trends in the evolution of their pollinaria.
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  • 88
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 135-140 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Poaceae =Gramineae ; Festuca sect. ; Bovinae ; Seed protein electrophoresis ; morphology ; numerical analysis ; systematics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Seed protein electrophoresis confirms the existence of polymorphism among hexaploid populations ofFestuca arundinacea. Both protein and morphological results suggest thatF. pratensis andF. arundinacea should retain independent specific status. High protein homology of these two species withF. gigantea points towards phylogenetic links between these taxa.
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  • 89
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 149-153 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Cactaceae ; Gymnocalycium subgen ; Pirisemineum ; subgen. nov. ; Flora of Bolivia ; Argentina and Paraguay
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A new subgenus ofGymnoalycium is described; its distribution and position within the genus are briefly discussed.
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  • 90
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 211-216 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Araceae ; Arum spp. div. ; Pollen ; scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A preliminary report is given on the pollen morphology of eight species of the genusArum examined by scanning electron microscopy. The pollen grains are spheroidal and their sporoderm sculpture possesses spines. The species vary mainly in number, shape and distribution of the spines on the pollen surface and these characters can be used to differentiate them.
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  • 91
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 205-210 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Poaceae (Gramineae) ; Hordeum bulbosum ; H. murinum ; Seed collections ; lodicule characters ; spikelet morphology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract It is sometimes necessary to identify eitherH. bulbosum orH. murinum on the basis of the inflorescence or “seeds” alone. The majority of taxonomic keys use the presence of swollen basal culms for the former against the annual habit for the latter. Confusion is due to similarities in inflorescences and spikelet morphology. Lodicules which always persist and are present beside the fruit in a mature caryopsis, and other characters such as the awns of the lemmas of the lateral spikelets enable conclusive distinction.
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  • 92
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Pollination ecology ; visitation rates ; pollination along altitudinal gradient ; Flora of Chile, of the Andes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Visitation rates and mean numbers of visits per flower per day are determined at three altitudinal levels (2 200–3 600 m) in the high Andes of central Chile from quantified observations of flowers visitors to a total of 134 species of plants, studied over three flowering seasons. Significant altitudinal decreases in the mean no. visits/flower/minute and per day were recorded, with Level III flowers, on the average, being pollinated only about 1/2 as frequently and to as low as 1/4 as frequently in certain months, as Level I flowers. Visitation rates are generally highest in early and mid-summer at all altitudes. — The lower visitation rates at the higher elevations are due to lower insect abundance relative to plant resources and lower levels of activity for the insects present, stemming from the generally lower ambient temperatures. Seasonal differences in visitation rates may also be related to differences in insect abundance levels. The total probability of ovule pollination cannot be estimated directly from the pollination rates, because stigmas are probably receptive for longer periods at the higher elevations. When differences in the duration of stigma receptivity are estimated from differences in flower lifelength, the probability of pollination in Level III is not very different from that expected in Level I. However, for some months, the deficit in Level III may still be substantial. — These results underline the inherent dangers of predicting pollination possibilities directly from pollination rates. They also suggest that the impoverished pollination conditions assumed in hypotheses predicting higher amounts of self-compatibility at high altitudes might not be justified for all high temperate mountains.
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  • 93
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 233-239 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Palmae ; Arecaceae ; Cocosoideae ; Attalea ; Scheelea ; Orbignya ; Flavones ; flavonols ; flavone C-glycosides ; flavonoid sulphates ; chemosystematics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In a flavonoid survey of direct and hydrolysed leaf extracts of sixteenAttalea, sevenScheelea and fourOrbignya species free tricin, tricin 7-glycosides, tricin 5-glucoside and flavone C-glycosides were the most frequent constituents; present in 100, 89, 70, and 81% of species, respectively. Luteolin, quercetin and isorhamnetin were each found in only 15% of the sample. The present results confirm the findings of a previous survey thatAttalea, Scheelea andOrbignya are chemically heterogeneous with as much variation between species as between genera. Furthermore, threeAttalea species,A. allenii, A. guaranitica andA. victoriana showed some infraspecific variation. On the other hand all three accessions ofA. ferruginea and six ofA. geraensis examined gave identical flavonoid profiles. The results support the view thatA. geraensis andA. guaranitica are closely related but do not support the suggested close relationship based on morphology betweenA. oleifera, A. burretiana andA. piassabossu.
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  • 94
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 253-286 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Gentianaceae ; Centaurium ; Blackstonia ; Secoiridoid glucosides ; xanthories ; chemotaxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Methanolic extracts from aerial parts and capsules of plants of 5 populations ofBlackstonia perfoliata and 99 populations of nine European and two AmericanCentaurium species (Gentianaceae) have been screened by means of TLC for the secoiridoid glucosides: sweroside, swertiamarin, gentiopicroside and the m-hydroxybenzoyl esters of sweroside, namely centapicrin, desacetylcentapicrin, decentapicrin A and B as well as for the xanthones: 1,8-dihydroxy-3,5-dimethoxyxanthone, 1,8-dihydroxy-3,7-dimethoxyxanthone, 1,8-dihydroxy-3,5,6,7-tetramethoxyxanthone and xanthone-β-mono-glucosides. The taxonomical significance of the results is discussed. On the basis of chemotaxonomical evidence twoCentaurium species,C. pulchellum andC. tenuiflorum, are placed in sect.Parviflora instead of sect.Centaurium subsect.Parviflora.
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  • 95
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 287-297 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Liliaceae ; Hyacinthaceae ; Scilloideae ; Ornithogalum ; O. umbellatum ; O. angustifolium ; Polyploid complex ; pollen shape ; pollen fertility ; seed formation ; seedlings ; weather circumstances
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The pollen fertility and seed formation of six species of theOrnithogalum umbellatum/angustifolium complex and of seven related species were studied. Four types of pollen grains could be recognized. The pollen fertility varied greatly in this complex and is not related to the ploidy level. The seed formation ofO. umbellatum showed an adaptation to a subcontinental-Mediterranean climate, that ofO. angustifolium to an Atlantic climate. In both cases raindrops seem to be important for pollination, in view of the absence of insect pollinators. After open pollination 113 seedlings were obtained in four species. Their chromosome numbers were determined. Nearly all the cultivated seedlings were aneuploid, which points to a positive selection of euploids in nature, because aneuploid individuals are rare in the wild.
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  • 96
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 149 (1985), S. 299-302 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Liliaceae ; Wurmbea ; Monocliny ; andromonoecy ; morph ratios ; seed set ; ovule production
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A population ofWurmbea dioica subsp.alba in Western Australia contained monoclinous and andromonoecious individuals in roughly equal proportions. The average number of flowers per inflorescence for the former was 2.6 and for the latter 2.9, with its terminal flower staminate. Ovule number and seed production per perfect flower of both morphs decreased progressively from lower to upper flowers in the inflorescence. Two-flowered monoclinous individuals had a greater percentage of ovules maturing to seed than did threeflowered ones, but seed production of the latter exceeded that of the former. No differences in percent seed set or in seed production were noted for similar individuals of andromonoecious individuals. Although monoclinous and andromonoecious plants apparently contributed equally to the pollen pool, seed production of the former exceeded that of the latter. Thus, the energetic costs of monocliny exceed those of andromonoecy in this population.
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  • 97
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 150 (1985), S. 83-88 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Epicuticular layer ; leaf resins ; flavonoid aglycones ; distribution ; arid habitat ; ecological functions ; chemotaxonomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Flavonoids generally occur in higher plants as water-soluble glycosides. However, lipophilic flavonoid aglycones can be excreted by glandular trichomes or extruded through the cuticle. They are accumulated on the plant surface as constituents of leaf resins or thin epicuticular layers. External flavonoid aglycones are found in various families throughout the higher plants, but appear to be most abundant in theAsteraceae. They occur relatively frequently in plants of (semi-)arid habitats. It is advantageous for chemotaxonomic studies that the structural diversity of exudate flavonoids is normally greater than that of the tissue glycosides in these plants.
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  • 98
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 150 (1985), S. 143-163 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Asteraceae ; Compositae ; Inuleae ; Antennaria ; Polyploidy ; compilospecies ; agamospermy ; dioecy ; agamic complex ; phenetics ; numerical taxonomy ; interspecific hybrids ; morphology ; origins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract TheAntennaria neodioica polyploid agamic complex is a polymorphic species occurring across North America mainly north of the terminal margin of the Wisconsin glacier. This taxonomically difficult group has recently been treated as consisting of the four subspeciesA. neodioica subsp.canadensis, subsp.howellii, subsp.neodioica, and subsp.petaloidea. TheA. neodioica agamic complex has been considered of hybrid origin with several sexual diploid species constituting its parentage. Crosses were made among five sexual diploid species ofAntennaria, morphologically similar toA. neodioica s.l., in an attempt to discover its origins. Representative specimens of the five diploid species,A. neodioica s. l., and the synthetic interspecific F1 hybrids were subjected to various analyses including PCA, cluster (UPGMA), and discriminant analyses. Results suggest that theA. neodioica complex is of multiple hybrid origin involving the four diploid speciesA. neglecta, A. plantaginifolia, A. racemosa, andA. virginica. BecauseA. neodioica is the result of diverse origin it is more desirable to consider the agamic complex as a separate, distinct species from its sexual diploid relatives. Several morphological characters in the diploid species were determined to be polygenically inherited.
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  • 99
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 150 (1985), S. 165-177 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Annonaceae ; Bocageopsis ; Ephedranthus ; Malmea ; Unonopsis ; Pollen ultrastructure ; exine differentiation ; non-columellate and columellate exine ; systematics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The four genera investigated show solitary sulcate pollen grains. The structure and sculpture of the sporoderm is very similar inBocageopsis andUnonopsis, and supports the close relationship of both genera; their flower morphology also is very similar. In contrast, the sporoderm ofEphedranthus, and especially that ofMalmea, is different in some characters and suggests more remote relationships; this is also confirmed by differences in flower morphology. Within this group of genera a distinct exine progression can be recognized from non-columellate (Malmea) to granular (Unonopsis guatterioides), further to somewhat irregularly (Bocageopsis, someUnonopsis species), and finally to very regularly columellate (Ephedranthus). The sculpture of the tectum varies from a reticulum with large lumina (Malmea) to one with small performations (Ephedranthus). Within theAnnonaceae the genusMalmea is among the most primitive in respect to pollen structure. The sulcus of the four genera is very large and runs over 1/3 of the pollen grain. It is characterized by a reduction of the exine and a bulgy thickening of the intine.
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  • 100
    Digitale Medien
    Digitale Medien
    Springer
    Plant systematics and evolution 150 (1985), S. 119-141 
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Annonaceae ; Guatteria ; Guatteriopsis ; Guatteriella ; Heteropetalum ; Pollen ultrastructure ; chromosome analysis ; Giemsa C-banding ; sequential fluorochrome staining ; leaf surface micromorphology ; evolution in primitive angiosperms
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Guatteria, Guatteriopsis, Guatteriella andHeteropetalum share the same conspicuous pollen type which is new for theSpermatophyta. It is zonoaperturate with a folded aperture region and an extremely reduced exine. First chromosome counts and karyotype analyses forGuatteriopsis (4 species investigated) andGuatteriella (1 species) are identical with those ofGuatteria (19 species seen): 2n = 28. The genome is characterized by diploidization and partly telocentric chromosomes. Sequentially Giemsa C- and fluorochrome banded chromosomes and interphase nuclei are described. The cuticular folding pattern is distinct forHeteropetalum only. Growth forms and ecology are reported for many species. The evolutionary pattern of theGuatteria group is discussed and compared with other genera and families.
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