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  • Articles  (52)
  • Electron microscopy  (27)
  • Wheat  (25)
  • Springer  (52)
  • American Geophysical Union (AGU)
  • Annual Reviews
  • 1980-1984  (52)
  • 1935-1939
  • 1982  (52)
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  • Articles  (52)
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  • Springer  (52)
  • American Geophysical Union (AGU)
  • Annual Reviews
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  • 1980-1984  (52)
  • 1935-1939
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  • 1
    Electronic Resource
    Electronic Resource
    Heat of fusion and lamellar structure of polyethylene single crystal mats (1982)
    Springer
    Colloid & polymer science 260 (1982), S. 564-569 
    Details
    ISSN: 1435-1536
    Keywords: lin. Polyethylene ; Single crystals ; Heat of Fusion ; DSC ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Recently published results for solution crystallized PE single crystals have shown, that the experimental heat of fusionΔH * is higher, if the solvent is exchanged to silicon oil (oil suspension samples) as compared with dried mats. This has been interpreted by the collapse of the original hollow pyramids during drying, inducing lateral defects within the lamellae. The present investigation does not confirm this unexpected result.ΔH * of dried mats (T c 66 to 91 °C) and of the corresponding oil suspension samples agree within the rather small limits of experimental error. The crystallinities as derived fromΔH *, density or WAXS are in excellent agreement. SEM micrographs of cold fractured dried mats show their spongy macromorphology, but TEM micrographs of stained ultra-thin sections reveal the lamellar morphology of the walls, consisting of curved lamellae and stacked hollow pyramides. If a dried mat is sintered at room temperature, a dense transparent film is obtained with a rather regular stacked morphology of large flat lamellae.ΔH * of these films agrees with that of the original mat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01422587
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence for inhibition of chitin synthesis by nikkomycin (1982)
    Springer
    Development genes and evolution 191 (1982), S. 205-207 
    Details
    ISSN: 1432-041X
    Keywords: Chitin inhibition ; Nikkomycin ; Cuticle ; Electron microscopy ; Epilachna varivestis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleoside antibiotic nikkomycin has proved to be an effective inhibitor of chitin synthesis in the Mexican bean beetleEpilachna varivestis. Ultrastructural investigations show defects in the procuticular area after nikkomycin application which suggest the complete absence of chitin. A cuticle like this is inflexible and too brittle to satisfy its normal function as an exoskeleton. The individuals are not able to free themselves from the exuvia and finally die. Therefore nikkomycin seems to be a potential insecticide with high specifity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848337
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative studies onChlorella cell walls: Induction of protoplast formation (1982)
    Springer
    Archives of microbiology 132 (1982), S. 10-13 
    Details
    ISSN: 1432-072X
    Keywords: Calcofluor White ; Cell wall structure ; Chlorella ; Electron microscopy ; Protoplast ; Ruthenium Red
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Among 12 strains ofChlorella ellipsoidea, C. vulgaris, andC. saccharophila tested, 4 strains (1,C. ellpsoidea; 2,C. vulgaris; 1,C. saccharophila) formed osmotically labile protoplasts after treatment with mixtures of polysaccharide degrading enzymes. The relationship between enzymatical digestibility and structure or composition ofChlorella cell walls were studied by electron microscopy and staining techniques with some specific dyes. The cell wall structures of the 12Chlorella strains were grouped into three types: (1) with a trilaminar outer layer, (2) with a thin outer monolayer, and (3) without an outer layer. Protoplasts were formed only from the strains with a cell wall of Type 2. In the strains with a cell wall of Type 1, the outer layer protected the inner major microfibrillar layer against enzymatic digestion. The cell wall of Type 3 was totally resistant to the enzymes; the chemical composition of the cell wall would be somewhat different from that of other types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690809
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  • 4
    Electronic Resource
    Electronic Resource
    Thylakoid centers: Structures associated with the cyanobacterial photosynthetic membrane system (1982)
    Springer
    Archives of microbiology 133 (1982), S. 97-99 
    Details
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Thylakoid centers ; Photosynthetic membranes/thylakoids ; Membranes ; Membrane biogenesis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An ultrastructural study of four cyanobacteria (Anabaena cylindrica, Dermocarpa violaceae, Gleocapsa alpicola, Pleurocapsa minor) indicates the presence of previously undescribed thylakoid centers from which photosynthetic membranes (thylakoids) radiate. These peripherally located thylakoid centers are cylinders 30 nm wide by 320 nm long, consisting of globular subunits oriented in nonparallel stacked arrays. Thylakoids are attached to the outer surface of the cylinder along its longitudinal axis. Thylakoid centers appear to be functionally significant due to their structure, location and thylakoid association.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413518
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  • 5
    Electronic Resource
    Electronic Resource
    Cell wall degradation ofStaphylococcus aureus by lysozyme (1982)
    Springer
    Archives of microbiology 131 (1982), S. 116-123 
    Details
    ISSN: 1432-072X
    Keywords: Cell wall ; Wall degradation ; Lysozyme ; Autolysines ; Electron microscopy ; Staphylococcus aureus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In contrast to former findings lysozyme was able to attack the cell walls ofStaphylococcus aureus under acid conditions. However, experiments with14C-labelled cell walls and ribonuclease indicated that, under these conditions, lysozyme acted less as an muralytic enzyme but more as an activator of pre-existing autolytic wall enzymes. Electron microscopic studies showed that under these acid conditions the cell walls were degraded by a new mechanism (i.e. “attack from the inside”). This attack on the cell wall started asymmetrically within the region of the cross wall and induced the formation of periodically arranged lytic sites between the cytoplasmic membrane and the cell wall proper. Subsequently, a gap between the cell wall and the cytoplasmic membrane resulted and large cell wall segments became detached and suspended in the medium. The sequence of lytic events corresponded to processes known to take place during wall regeneration and wall formation. In the final stage of lysozyme action at pH 5 no cell debris but “stabilized protoplasts” were to be seen without detectable alterations of the primary shape of the cells. At the same time long extended ribbon-like structures appeared outside the bacteria. The origin as well as the chemical nature of this material is discussed. Furthermore, immunological implications are considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01053992
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  • 6
    Electronic Resource
    Electronic Resource
    Investigation of a preferentially transmitted Aegilops sharonensis chromosome in wheat (1982)
    Springer
    Theoretical and applied genetics 61 (1982), S. 27-33 
    Details
    ISSN: 1432-2242
    Keywords: Preferential transmission ; In situ hybridization ; C-banding ; Aegilops sharonensis ; Wheat ; Addition ; Substitution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An attempt to produce a set of addition lines of Aegilops sharonensis to the wheat variety ‘Chinese Spring’ produced only one addition line. This was due to preferential transmission of one chromosome from Ae. sharonensis. This chromosome was studied in detail by established cytological methods of chromosome observation and by the newer techniques of C-banding and in situ hybridization of a cloned DNA sequence. The chromosome was found to be partially homologous to an Ae. sharonensis chromosome of similar behaviour in another wheat addition line. The incomplete homology of the two Ae. sharonensis chromosomes was due to the presence of a translocated segment of a wheat chromosome. — Substitution lines of the Ae. sharonensis chromosome for wheat homoeologous group 4 were produced and the Ae. sharonensis chromosome thereby designated 4 S l .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261506
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  • 7
    Electronic Resource
    Electronic Resource
    Preferential C-banding of wheat or rye chromosomes (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 227-233 
    Details
    ISSN: 1432-2242
    Keywords: C-banding ; Wheat ; Rye ; Triticale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using different stains, wheat chromosomes could be distinguished from rye chromosomes by preferential staining. C-bands of rye chromosomes were preferentially stained with Giemsa while those of wheat chromosomes were preferentially stained with either Leishman or Wright stain. Preferential staining aids the identification of wheat and rye chromosomes and chromosome segments and in particular the recognition of wheat/rye chromosome substitutions and translocations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304000
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  • 8
    Electronic Resource
    Electronic Resource
    Two NAD-dependent alcohol dehydrogenases (E.C. 1.1.1.1) in callus cultures of wheat, rye and triticale (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 45-48 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Triticale ; Callus cultures ; Alcohol dehydrogenase ; Isoenzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two NAD-dependent alcohol dehydrogenases ADH-1 and ADH-2, under independent genetic control of genes designated as Adh-1 and Adh-2 located on chromosomes 4A, 4B and 4D, have been reported in aestivum wheat (Hart 1980). Only ADH-1 is expressed in developing seeds, dry seeds, pollen and germinating seedlings. ADH-2 can be induced in seedling roots or shoots under conditions of partial anaerobiosis or by certain chemicals. Expression of ADH-1 and ADH-2 isoenzymes was investigated in undifferentiated calli from aestivum and durum wheats, rye, triticale and also in in vitro regenerated roots and leaves from aestivum cultures. Wheat callus cultures originating from seed, mature and immature embryos, mesocotyl and root, as well as cultures grown on media containing different supplements did not show any variation in the overall expression of ADH-1 or ADH-2, although differences in the band intensities were observed. The callus isoenzyme pattern was similar to that observed in roots under anaerobic conditions. Both ADH-1 and ADH-2 were expressed in in vitro regenerated roots but were absent in regenerated leaves. Expression of ADH-1 and ADH-2 in wheat calli seems to be related to the type of differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276281
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  • 9
    Electronic Resource
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    Predicting the frequencies of transgressive segregants for yield and yield components in wheat (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 127-134 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Genetical analysis ; Cross prediction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetical analysis of the F2 triple test cross design combined with conventional early generations was used to elucidate the genetical control of yield and yield components in two crosses of winter wheat. From estimates of the additive, {d}, and additive X additive, {i}, components of means, together with the additive genetical variance, D, predicted frequencies of recombinant inbred lines that would transgress the parental range were calculated for each cross. The accuracy of predictions was evaluated by comparing expected frequencies with observed numbers in populations of F6 lines previously developed by single seed descent. For both crosses and all characters where an adequate genetical model was found to explain the observed variation between the early generations, good agreement between predicted and observed frequencies of transgressive segregants was obtained. Furthermore, for characters exhibiting significant epistasis, allowance for additive X additive {i} epistasis in the prediction equations was sufficient to allow for skewness of the recombinant inbred population. These results demonstrate that cross performance in wheat can be predicted from genetical analysis of early generations, and the value of this approach in breeding new varieties is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293344
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  • 10
    Electronic Resource
    Electronic Resource
    The Tom Thumb dwarfing gene, Rht3 in wheat, I. Reduced pre-harvest damage to breadmaking quality (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 121-126 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Quality ; Dwarfism ; Gibberellin ; α-amylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of the Tom Thumb dwarfing gene, Rht3, on the quality and quantity of grain α-amylase produced during germination and by induction with exogenous gibberellic acid are described. In a season conducive to high sprouting damage the gene reduced α-amylase levels in the field by 77%. Selection among random Rht3 genotypes showed that other genetic factors can be combined with the dwarfing gene to further increase sprouting damage resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293343
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  • 11
    Electronic Resource
    Electronic Resource
    Genetic control of 6-phosphogluconate dehydrogenase (6-PGD) isozymes in cultivated wheat and rye (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 317-320 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Phosphogluconate dehydrogenase ; Genetic control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 6-phosphogluconate dehydrogenase (6-PGD) zymogram phenotypes of wheat, rye and their aneuploid derivatives were determined. Two genes involved in the production of 6-PGD isozymes were located on chromosome arms CRL (4 RL) and FRL (6 RL) of “Imperial” rye. On the basis of differential interactions between wheat and rye chromosomes, evidence was obtained that genes located on chromosomes 6 A, 6 BL and 7 BL control 6-PGD isozyme activities in ‘Chinese Spring’ wheat. The wheat and rye 6-PGD zymogram phenotypes were indicative of homoeologous relationships between rye chromosome 6 RL to wheat chromosomes of group 6, and rye chromosome 4 RL to wheat chromosomes of group 7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275094
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  • 12
    Electronic Resource
    Electronic Resource
    The purification and characterization of homologous high molecular weight storage proteins from grain of wheat, rye and barley (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 329-336 
    Details
    ISSN: 1432-2242
    Keywords: HMW storage prolamins ; Wheat ; Rye ; Barley ; Characterization and purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Homologous high molecular weight storage prolamins were purified from grain of wheat, rye and barley using combinations of gel filtration, ion-exchange chromatography and preparative isoelectric focusing. Sodium dodecylsulphate polyacrylamide gel electrophoresis showed that the components were single bands with apparent mol.wts. of above 100,000. Molecular weights determined by sedimentation equilibrium ultracentrifugation were considerably lower; 54,700, 67,600 and 69,600 for the components from barley, rye and wheat respectively. Amino acid analysis showed the presence of 13.6 to 16.5 mol% glycine, 29.6 to 34.0 mol% glutamate + glutamine, 11.4 to 13.7 mol% proline and a total of 4.0 to 5.7 mol% basic amino acids. Automated N-terminal amino acid sequencing of the component from wheat showed the presence of cysteine residues at positions 5 and 10, and this is discussed in relation to the possible role of these proteins in the visco-elastic gluten network.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275097
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  • 13
    Electronic Resource
    Electronic Resource
    C-banded wheat chromosomes in wheat and triticale (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 39-47 
    Details
    ISSN: 1432-2242
    Keywords: C-banding ; Wheat ; Triticale ; Heterochromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The C-banding patterns of wheat chromosomes in 7 hexaploid triticale and 7 wheat genotypes are described and compared. All 14 wheat chromosome pairs were individually identified in the triticales and a tetraploid wheat, and all the B and two A genome chromosome pairs in the hexaploid wheat genotypes. Little variation was found between genotypes in the distribution of C-bands but considerable variation was found in their size, total number and total length.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303488
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  • 14
    Electronic Resource
    Electronic Resource
    Relationships of Agropyron intermedium chromosomes determined by chromosome pairing and alcohol dehydrogenase isozymes in common wheat background (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 213-217 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Agropyron ; Chromosome pairing ; Alcohol dehydrogenase isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationships of Agropyron intermedium chromosomes in two wheat-Agropyron addition series were determined. Chromosome pairing behaviour revealed that the alien chromosome in lines TAF-2 and L7 of ‘Vilmorin’-A. intermedium set are homologous to the alien chromosomes in lines P and C of the ‘Caribo’-A. intermedium set respectively. Localization of alcohol dehydrogenase isozyme genes in ‘Vilmorin’-Agropyron addition line L4 and in ‘Caribo’-Agropyron line O indicated relationships with wheat chromosomes of homoeologous group 4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303996
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  • 15
    Electronic Resource
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    The wheat ribosomal DNA spacer region: Its structure and variation in populations and among species (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 337-348 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; rDNA ; Sequence ; Populations ; Variation ; Spacer region ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The wheat rDNA clone pTA250 was examined in detail to provide a restriction enzyme map and the nucleotide sequence of two of the eleven, 130 bp repeating units found within the spacer region. The 130 bp units showed some sequence heterogeneity. The sequence difference between the two 130 bp units analysed (130.6 and 130.8) was at 7 positions and could be detected as a 4 °C shift in Tm when heterologous and homologous hybrids were compared. This corresponded to a 1.2% change in nucleotide sequence per ΔTm of 1 °C. The sensitivity of the Tm analysis using cloned sequences facilitated the analysis of small sequence variations in the spacer region of different Triticum aestivum cultivars and natural populations of T. turgidum ssp. dicoccoides (referred to as T. dicoccoides). In addition spacer length variation was assayed by restriction enzyme digestion and hybridization with spacer sequence probes. Extensive polymorphism was observed for the spacer region in various cultivars of T. aestivum, although within each cultivar the rDNA clusters were homogeneous and could be assigned to particular chromosomes. Within natural populations of T. dicoccoides polymorphism was also observed but, once again, within any one individual the rDNA clusters appeared to be homogeneous. The polymorphism, at the sequence level (assayed by Tm analysis), was not so great as to prevent the use of spacer sequence variation as a probe for evolutionary relationships. The length variation as assayed by restriction enzyme digestion did not appear to be as useful in this regard, since its range of variation was extensive even within populations of a species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303905
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  • 16
    Electronic Resource
    Electronic Resource
    Chromosome and nucleotide sequence differentiation in genomes of polyploid Triticum species (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 349-360 
    Details
    ISSN: 1432-2242
    Keywords: Chromosomes ; Nucleotides ; Evolution ; Polyploids ; Triticum ; Heterochromatin ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nature of genome change during polyploid evolution was studied by analysing selected species within the tribe Triticeae. The levels of genome changes examined included structural alterations (translocations, inversions), heterochromatinization, and nucleotide sequence change in the rDNA regions. These analyses provided data for evaluating models of genome evolution in polyploids in the genus Triticum, postulated on the basis of chromosome pairing at metaphase I in interspecies hybrids. The significance of structural chromosome alterations with respect to reduced MI chromosome pairing in interspecific hybrids was assayed by determining the incidence of heterozygosity for translocations and paracentric inversions in the A and B genomes of T. timopheevii ssp. araraticum (referred to as T. araraticum) represented by two lines, 1760 and 2541, and T. aestivum cv. Chinese Spring. Line 1760 differed from Chinese Spring by translocations in chromosomes 1A, 3A, 4A, 6A, 7A, 3B, 4B, 7B and possibly 2B. Line 2541 differed from Chinese Spring by translocations in chromosomes 3A, 6A, 6B and possibly 2B. Line 1760 also differed from Chinese Spring by paracentric inversions in arms 1AL and 4AL whereas line 2541 differed by inversions in 1BL and 4AL (not all chromosomes arms were assayed). The incidence of structural changes in the A and B genomes did not coincide with the more extensive differentiation of the B genomes relative to the A genomes as reflected by chromosome pairing studies. To assay changing degrees of heterochromatinization among species of the genus Triticum, all the diploid and polyploid species were C-banded. No general agreement was observed between the amount of heterochromatin and the ability of the respective chromosomes to pair with chromosomes of the ancestral species. Marked changes in the amount of heterochromatin were found to have occurred during the evolution of some of the polyploids. The analysis of the rDNA region provided evidence for rapid “fixation” of new repeated sequences at two levels, namely, among the 130 bp repeated sequences of the spacer and at the level of the repeated arrays of the 9 kb rDNA units. These occurred both within a given rDNA region and between rDNA regions on nonhomologous chromosomes. The levels of change in the rDNA regions provided good precedent for expecting extensive nucleotide sequence changes associated with differentiation of Triticum genomes and these processes are argued to be the principal cause of genome differentiation as revealed by chromosome pairing studies.
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    URL: http://dx.doi.org/10.1007/BF00303906
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  • 17
    Electronic Resource
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    Sensory organs inIps typographus (Insecta: Coleoptera) — Fine structure of antennal sensilla (1982)
    Springer
    Protoplasma 111 (1982), S. 206-214 
    Details
    ISSN: 1615-6102
    Keywords: Bark beetle ; Sensilla ; Chemoreceptors ; Mechanoreceptors ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The antennal sensilla inI. typographus are almost exclusively confined to the flattened terminal flagellar segment. The sensillar types have distinct distribution patterns in the three areas where they are found. Judging from the ultrastructural characteristics the following functions can be assigned to the sensillar types: chemoreception, single-walled and double-walled sensilla; chemoreception/mechanoreception, terminal-pore sensillum. Moreover there are two types of mechanoreceptors, one of which is connected to a bristle, whereas the other terminates within the cuticle of the flagellar segment.
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    URL: http://dx.doi.org/10.1007/BF01281968
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  • 18
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    Plant regeneration from cultured immature embryos and inflorescences ofTriticum aestivum L. (wheat): Evidence for somatic embryogenesis (1982)
    Springer
    Protoplasma 110 (1982), S. 95-105 
    Details
    ISSN: 1615-6102
    Keywords: Cereals ; Immature embryo ; Inflorescence ; Plant regeneration ; Somatic embryogenesis ; Triticwn aestivum L. ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tissue cultures ofTriticum aestivum L. (wheat) initiated from young inflorescences and immature embryos possessed the potential for regeneration of whole plants. Both a friable and a compact type of callus were produced on Murashige and Skoog's medium with 2 mg/l 2,4-dichlorophenoxyacetic acid. The friable callus contained meristematic centers in which the peripheral cells ceased dividing, elongated, and could be easily separated. Roots were frequently formed in this type of callus. The compact, yellowish, and nodular callus arose from the epithelial and sub-epithelial cells of the embryo scutellum, and the rachis and glumes of the young inflorescence. Such callus had a smooth surface and characteristic chlorophyllous areas. Plants were regenerated only from the compact callus. The first sign of differentiation in the compact callus was the formation of a cleft or notch on the smooth surface, followed by the appearance of trichomes and the direct development of leafy structures which were not associated initially with any shoot meristems. Multiple shoots subsequently arose at the bases of the leafy structures, which are considered modifications of the scutellum, a definitive part of the cereal embryo. Accordingly, we suggest that while typical bipolar embryos are generally not formed, plant regeneration nevertheless takes place through embryogenesis and the precocious germination of the embryoids. Plants regenerated from immature embryo and inflorescence cultures were grown to maturity in soil, and were shown to have the normal chromosome number of 2n=6x=42.
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    URL: http://dx.doi.org/10.1007/BF01281535
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  • 19
    Electronic Resource
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    Natural senescence of the root cortex of spring wheat in relation to susceptibility to common root rot (Cochliobolus sativus) and growth of a free-living nitrogen-fixing bacterium (1982)
    Springer
    Plant and soil 66 (1982), S. 13-20 
    Details
    ISSN: 1573-5036
    Keywords: Acridine orange ; Cochliobolus sativus ; Common root rot ; Nitrogen fixation ; Nuclear staining ; Roots ; Senescence ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Natural senescence of the root cortex was assessed by nuclear staining, for cultivars and chromosome substitution lines of spring wheat known to differ in (1) susceptibility to common root rot, (2) total rhizosphere populations and (3) ability to support growth of a free-living nitrogen-fixing bacterium. Together, three root rot susceptible wheat lines showed significantly more cortical senescence than did three resistant lines; the susceptible lines also support larger rhizosphere populations. The wheat line that supports growth of a nitrogen-fixing bacterium showed significantly less cortical death than did any other line. Substitution of chromosome pairs 5B or 5D between the parent cultivars Rescue and Cadet substantially altered the amount of root cortex death, which is thus genetically determined. It is suggested thatCochliobolus sativus and other weak parasites benefit from early natural senescence of the root cortex, and that the degree of susceptibility or resistance of wheat lines to common root rot is at least partly determined by differences in cortical senescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02203397
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  • 20
    Electronic Resource
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    Use of triple-labelled nutrient solution to study the effects of root-infecting fungi on uptake of potassium, calcium and phosphorus by wheat (1982)
    Springer
    Plant and soil 66 (1982), S. 405-410 
    Details
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; Hydroponics ; Ion uptake ; Radionuclides ; Root pathogens ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Triple-labelled nutrient solution was used to compare the effects of seven root-infecting fungi on uptake of K, Ca and P by wheat. Plants grown in sand or hydroponic culture were transferred to solutions that contained42K,45Ca and32P for 24 h, then dried, ashed and digested in 6M HCl. To distinguish radiation emitted by42K,45Ca and32P plant digests were counted on two channels of a liquid scintillation counter immediately and 7 days later, after the decay of42K radiation. Plants infected byGaeumannomyces graminis took up and translocated less K, Ca and P to their shoots than uninfected plants. Other root-infecting fungi had little effect on uptake of these ions.
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    URL: http://dx.doi.org/10.1007/BF02183807
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    Effects of waterlogging on young wheat plants (Triticum aestivum L.) and on soil solutes at different soil temperatures (1982)
    Springer
    Plant and soil 69 (1982), S. 311-326 
    Details
    ISSN: 1573-5036
    Keywords: Aeration ; Calcium ; Carbon dioxide ; Ethylene ; Nitrate ; Nitrite ; Nitrous oxide ; Oxygen ; Potassium ; Roots ; Triticum aestivum ; Waterlogging ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We report a study of the mechanism by which the response of plants to waterlogging can be modified by soil temperature. Wheat was grown initially in well-aerated soil in a controlled environment room before the soil was flooded with aerated, deionized water. The soil temperature was maintained constant in the range 6–18°C while the air temperature was at 14°C. Waterlogging damage was greater in plants at the higher soil temperatures when the plants were compared at the same chronological age. However, when compared at the same growth stage, the response to soil temperature was little differenti.e. plants subjected to waterlogging for a long time at low soil temperatures exhibited a similar reduction in growth and other properties as those subjected briefly at higher temperatures. The concentration of dissolved oxygen in the soil solution declined rapidly at all temperatures, being almost zero after 36 h waterlogging. Temperature affected rates of change of the concentrations of dissolved carbon dioxide, ethylene, nitrous oxide, nitrite, nitrate, calcium and potassium. The importance of soil-and plant-determined properties in the waterlogging response of plants at different temperatures are discussed.
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    URL: http://dx.doi.org/10.1007/BF02372453
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    Effects of shading and powdery mildew infection on senescence of the root cortex and coleoptile of wheat and barley seedlings, and implications for root- and foot-rot fungi (1982)
    Springer
    Plant and soil 69 (1982), S. 401-411 
    Details
    ISSN: 1573-5036
    Keywords: Barley ; Gaeumannomyces graminis ; Nuclear staining ; Pseudocercosporella herpotrichoides ; Root rot ; Senescence ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Nuclear and cytoplasmic staining methods were used to study natural senescence of the root cortex and coleoptile of wheat and barley seedlings grown in glasshouse conditions. Coleoptiles of barley senesced more slowly than those of wheat, paralleling the known difference in rates of root cortex senescence in these cereals. The coleoptiles and root cortices of both cereals senesced more slowly in shaded than in unshaded conditions, but infection of the shoots of barley byErysiphe graminis had little effect on root cortex senescence. The results are discussed in relation to infection by root- and foot-rot fungi. Previous reports on the effects of illumination on take-all infection (Gaeumannomyces graminis) are explained. It is suggested that natural senescence of the coleoptile might affect establishment of infection by the eyespot fungus,Pseudocercosporella herpotrichoides, either directly or through the activities of competing microorganisms.
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    URL: http://dx.doi.org/10.1007/BF02372461
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    Effect of different Mg/Ca ratios and electrolyte concentrations in irrigation water on the nutrient content of wheat crop (1982)
    Springer
    Plant and soil 65 (1982), S. 63-71 
    Details
    ISSN: 1573-5036
    Keywords: Mg/Ca ratio ; Water quality ; Nutrient content ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study conducted in pots to evaluate the effect of different Mg/Ca ratios (2, 4, 8 and 16) and electrolyte concentrations (20 and 80 meq/l) at SAR 10 in irrigation water on the nutrient uptake and yield of wheat crop in two soils revealed that the average grain and dry matter yields of wheat decreased significantly with an increase in Mg/Ca ratio in irrigation water, but the magnitude of decrease was greater at higher electrolyte concentration than at lower electrolyte concentration. The concentration of Na in both straw and grain of wheat increased and that of K decreased with an increase in Mg/Ca ratio and electrolyte concentration of irrigation water, which led to higher Na/Ca and Na/K ratios in the plant. Further, the concentration of Ca and Mg both in straw as well as in grain increased with increasing electrolyte concentration of the irrigation water. An increasing proportion of Mg in saline irrigation water resulted in decreased concentration of Ca and increased concentration of Mg in both straw and grain of wheat crop. It was also noticed that the increasing proportion of Mg over Ca in the poor quality irrigation water increased the P content of both straw and grain of wheat crop.
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    URL: http://dx.doi.org/10.1007/BF02376803
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    Copper nutrition of wheat in relation to nitrogen and phosphorus fertilization (1982)
    Springer
    Plant and soil 65 (1982), S. 433-436 
    Details
    ISSN: 1573-5036
    Keywords: Copper-nutrition ; Nitrogen ; Phosphorus ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a pot culture study, copper addition to soil increased the crop yield only in presence of nitrogen. The latter increased the utilization of both native as well as applied copper but more that of applied. It also minimised the adverse effect of applied phosphorus on copper utilization. Phosphorus at the rate 45 ppm had the tendency of decreasing copper uptake by wheat if applied without nitrogen or with its low level.
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    URL: http://dx.doi.org/10.1007/BF02375066
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    Utility of phyllosphere N2-fixing micro-organisms in the improvement of crop growth (1982)
    Springer
    Plant and soil 68 (1982), S. 69-74 
    Details
    ISSN: 1573-5036
    Keywords: Azotobacter ; chroococeum ; Derxia ; gumnosa ; Klebsiella ; Nitrogen ; fixation ; Phyllosphere ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five highly active N2-fixing strains of Klebsiella isolated from the phyllosphere of tropical plants, KUPBR1, KUPBR2, KUPM, KUP4 and KUP6 along withAzotobacter vinelandii OP,A. chroococcum G40 andDerxia gumnosa were sprayed on the foliage of a high yielding wheat cultivar, Kalyansona. The strains of Klebsiella enhanced dry wt., chrorophyll and nitrogen content, and 1000 grain weight. Grain yield in inoculated plants varied between 19.9 and 25.09 quintals ha−1 against 24.34 quintals with urea. Best results were obtained with KUP4 which when applied at half dose was as effective as 52.5 kg Urea-N ha−1. Azotobacter and Derxia only slightly improved growth and nitrogen content of grain or straw.
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    URL: http://dx.doi.org/10.1007/BF02374728
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    Effect of long-term fertilization and cropping on the potassium supplying capacity of soils (1982)
    Springer
    Plant and soil 65 (1982), S. 375-382 
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    ISSN: 1573-5036
    Keywords: Activity ratio ; Free energy ; Pearl millet ; Potassium supplying capacity ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil samples from a long term field experiment, to study the K depletion pattern, were analysed for K. The values of supply parameters pK−1/2p (Ca+Mg) and free energy δG r 0 were calculated using the data on water soluble and exchangeable cations. Correlation coefficient values between pK−1/2p (Ca+Mg) vs. total K removed, K removal by pearl millet in 1980 and soil available potassium were positive and statistically significant. This indicated that these soils have reached the stage where they would start responding to potassium application. Also, negative but significant correlation of δG r 0 with pK−1/2p (Ca+Mg) and available K, were found. δG r 0 values were positive indicating the affinity of such soils for K and the values, for K fertilized plots were lower in comparison to plots where no K was applied. pK−1/2p (Ca+Mg) parameter was found to be a better measure of K availability over δG r 0 due to either fixation of K or greater contribution of nonexchangeable K fraction towards plant utilized K.
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    URL: http://dx.doi.org/10.1007/BF02375058
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    Response of barley and wheat to phosphorus in the presence of chloride and sulphate salinity (1982)
    Springer
    Plant and soil 66 (1982), S. 233-241 
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    ISSN: 1573-5036
    Keywords: Barley ; Chloride ; Phosphorus ; Salinity ; Sulphate ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The study was conducted in a greenhouse and under field conditions. In the greenoouse, barley was grown to maturity in pots on a sandy soil which contained 80 and 120 meq/l of chloride and sulphate dominant salts in its saturation extract, to which 0, 10, 25 and 50 ppm P were added. In the field study, wheat was grown on loamy sand soils having 0, 25, 50 and 75 kg/ha added P levels and irrigated with either Cl- or SO4-dominant saline waters (EC=15−19 mmhos/cm). The results of the greenhouse study indicated that at maturity barley straw and grain yield was significantly increased by 50 ppm of added P both on the non-saline control and the Cl-treatments. However, 25 ppm P was optimal on the SO4-treatments. The Cl content of plants was significantly decreased and S was increased with the increase in the P content of soil. A synergistic relation between the S and P content of barley shoots was observed. In the field study wheat grain yield responded significantly to P applications upto 50 kg/ha level on the Cl-site and there was no response to applied P on the SO4-site, although the former contained more Olsen's P than the latter. The results suggested that P requirement of wheat and barley was greater on Cl- than on SO4-salinity.
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    URL: http://dx.doi.org/10.1007/BF02183982
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    Effect of nitrogen management and soil moisture conservation practices on rainfed wheat (Triticum aestivum L.) (1982)
    Springer
    Plant and soil 66 (1982), S. 433-437 
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    ISSN: 1573-5036
    Keywords: Kaolin spray ; Mulch ; N-application ; Split application ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies revealed that the application of fertilizer nitrogen brought a significant increase in grain and straw yield of wheat. The significant effect was also noticed on such yield contributing characters like number of effective tillers per metre row length, spike length, and number of grains per spike. The increase of nitrogen level from 40 kg/ha to 80 kg/ha also brought a significant increase in yield and yield contributing characters. The application of entire dose of nitrogen at the time of sowing was as good as its split application. The application of nitrogen also influenced the nitrogen concentration of grains whereas, the other treatments did not influence the nitrogen concentration in grains or straw. The nitrogen treatments did not influence the moisture content of soil. The application of mulch or mulch+Kaolin resulted in significantly higher content of soil moisture in 0–15 cm soil depth as compared to control or Kaolin spray alone which was simultaneously reflected in yield and yield contributing characters.
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    Effect of soil salinity and water stresses on growth and content of nitrogen, chloride and phosphate of wheat and triticale (1982)
    Springer
    Plant and soil 68 (1982), S. 207-216 
    Details
    ISSN: 1573-5036
    Keywords: Calcareous soil ; Chloride ; Dry matter ; Nitrogen ; Phosphate ; Soil salinity ; Triticale ; Triticum aestivum L. ; Water stress ; Wheat ; X Triticosecale Wittmack
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three wheats and one triticale were grown, up to flowering stage, in pots on calcareous soil adjusted to a range of salinities (S1=3.5, S2=6, S3=8.5, and S4=11 mmhos/cm, 20°C, soilpaste extract) by adding solution consisting of 3∶2∶1 of Na-, Ca- and Mg chlorides in chemical equivalent amounts. Moisture in the pots was kept at 100% (W1), 40% (W2) and 20% (W3) of the available water. The vegetative growth, nitrogen and phosphate were affected by S and W treatments, chloride was affected only by S. The interaction S×W affected only dry weight. Varietal effect was observed between wheat as a group and triticale. Multiple quadratic regression equations of these properties on salinity and water revealed that the higher the available water the wider the range of tolerable salinity. Triticale was relatively more tolerant to water stress. Salinity increases Cl and decreases N, whereas water stress enhances N accumulation to a certain extent. However, in triticale at S3 and S4 the effect of water stress on N was overshadowed by the excessive salinity. This did not occur for the wheat (Florence). P trends were described. R2 for P was low (0.7435–0.3603) which made interpretations rather difficult.
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    URL: http://dx.doi.org/10.1007/BF02373706
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    Silica content ofTriticum aestivum L. in relation to humic acid concentrations in the soil (1982)
    Springer
    Plant and soil 68 (1982), S. 139-141 
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    ISSN: 1573-5036
    Keywords: Humic acids ; Silica ; Triticum aestivum L. ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The weathering of soil minerals by humic acids is linked with increased silica content of wheat plants.
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    URL: http://dx.doi.org/10.1007/BF02374735
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    Response to selection for grain yield and harvest index in F2, F3 and F4 derived lines of two wheat crosses (1982)
    Springer
    Euphytica 31 (1982), S. 139-150 
    Details
    ISSN: 1573-5060
    Keywords: Wheat ; Triticum aestivum ; wheat breeding ; selection ; grain yield ; harvest index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aspects of selection for yield and harvest index were investigated by simulating selection using data from random pedigreed F2, F3, F4 and F5 derived lines from two crosses grown in plots at two sites over two years. Improvement in yield through selection was obtained when the response was measured at the same site and in the same year as the selection. Selecting the best 10 per cent of F2 to F4 derived lines gave F5 derived lines that outyielded random selections by 19 to 53 per cent for one cross and 5 to 23 per cent for the second cross. These lines were 41 to 50 per cent better than the mid-parent in one cross, but were less than the mid-parent in the other cross. However, the response to selection when measured in a different year was little better than random selection. The effect of different sites also reduced the effectiveness of selection. Selection of harvest index in early generations for improvement of yield was ineffective when response was measured at the same site in the same year, or in different years. Contrary to some theoretical proposals, the same improvement in yield was obtained by selecting in early or late generations. While high yielding genotypes may be lost by delaying selection, this is counteracted by the better predictive value of late generations due to their greater homozygosity and homogeneity.
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    URL: http://dx.doi.org/10.1007/BF00028316
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    Cells and intercellular contacts in glomeruli and tubules of the frog kidney (1982)
    Springer
    Cell & tissue research 226 (1982), S. 589-608 
    Details
    ISSN: 1432-0878
    Keywords: Kidney (frog) ; Glomerulus ; Nephron ; Tight junctions ; Freeze-fracturing ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By the use of thin sections and freeze-fracture replicas the glomerular and tubular structures of the kidney of the frog (Rana esculenta) were studied with special reference to intercellular junctions. In the glomerulus the filtration barrier is of very variable thickness, and frequent tight and gap junctional contacts occur between podocyte processes. Although structurally less elaborate, the proximal tubule resembles its mammalian counterpart. In the initial part the tight junctions are relatively shallow but become very broad in the mid and distal portions of the proximal tubule. The proximal tubular cells are extensively linked by gap junctions. In some animals the shapes of the cells in the proximal and distal portions of the proximal tubule were markedly different. The distal tubule consists of two segments which differ mainly in the pattern of interdigitations and the structure of the zonulae occludentes. Similarities with the tight junctional morphology of the mammalian distal tubule are striking. In the first part of the distal tubule (diluting segment) a narrow band of parallel tight junctions is found closely resembling that found in the mammalian straight distal tubule; in the more distal part of the distal tubule, however, a broad band of anastomosing tight junctional strands exists, like the zonula occludens of the mammalian convoluted distal tubule. The connecting tubule displays cellular dimorphism: its wall contains a mixture of light and dark (flask) cells. The luminal and basolateral membranes of the flask cells are covered with numerous rod-shaped particles. The tight junctions of the connecting tubule are broad and increase in depth and number of strands along its length; they are typical of a very tight epithelium. In spite of several dissimilarities with phylogenetically younger kidneys our findings suggest that many structural principles of the mammalian kidney are also represented in the kidneys of amphibians. The structural-functional relationships are discussed.
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    Ultrastructure of endocrine cells in the stomach of two teleost fish Perca fluviatilis L. and Ameiurus nebulosus L. (1982)
    Springer
    Cell & tissue research 221 (1982), S. 657-678 
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    ISSN: 1432-0878
    Keywords: Stomach (Teleost) ; Endocrine cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the gastric mucosa of two teleost species, the perch (Perca fluviatilis) and the catfish (Ameiurus nebulosus) three endocrine cell types were found, located predominantly between the mucoid cells of the gastric mucosa. A fourth cell type is present in the gastric glands of catfish. Each cell type was defined by its characteristic secretory granules. Type-I cells were predominant in both fish. These cells contained round or oval granules with a pleomorphic core. The average diameter of granules was 400 nm for the perch and 270 nm for the catfish. Type-II cells of both species displayed small, highly osmiophilic granules about 100 nm in diameter. The secretory granules of type-III cells (260 nm in the perch and 190 nm in the catfish) were round or slightly oval in shape and were filled with a finely particulate electron-dense material. Type-IV cells of the catfish were found in the gastric glands only. Their cytoplasm was filled with homogeneous, moderately electron-dense granules averaging 340 nm in diameter. The physiological significance of these different morphological types of gastric endocrine cells requires further investigation.
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    Apical region of the crayfish retinula (1982)
    Springer
    Cell & tissue research 222 (1982), S. 409-415 
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    ISSN: 1432-0878
    Keywords: Crustacean compound eye ; Eighth retinular cell ; Crystalline tract ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The base of the crystalline tract, the distal part of the eighth retinular cell and its rhabdomer constitute a structural unit in the apical region of the retinula of Astacus fluviatilis and A. leptodactylus, shielded from the blood by a special covering cell.
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    Electron-microscopic localization of A2B5 cell surface antigen in monolayer cultures of murine cerebellum and retina (1982)
    Springer
    Cell & tissue research 224 (1982), S. 637-645 
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    ISSN: 1432-0878
    Keywords: Cell surface antigen ; Neurons ; Glia ; Electron microscopy ; Immunoperoxidase ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immuno-electron microscopy was performed on live, cultured, early postnatal cerebellar and retinal cells of the mouse to identify A2B5 antigenbearing elements. In cerebellar cultures, granule cells, some immature oligodendroglia, and astroblasts express A2B5 antigen on their cell surfaces. The typical features of astroblasts include large cisternae of the endoplasmic reticulum and a mixed population of intermediate-sized filaments and microtubules. Immature oligodendroglia cells express the antigen on their cell bodies and on procecesses filled with cytoplasm. Cytoplasm-free membranous whorls, however, are devoid of A2B5 antigen, but not of 0 or NS-1 antigens. In retinal cultures, A2B5 antigen is observed on differentiating neurons with the exception of photoreceptor cells as identified by ribbon synapses.
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    Survival of Pacinian corpuscles after denervation in adult rats (1982)
    Springer
    Cell & tissue research 224 (1982), S. 673-683 
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    ISSN: 1432-0878
    Keywords: Pacinian corpuscles, rat ; Denervation ; Sensory terminals ; Nerve degeneration ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of Pacinian corpuscles located on the crural interosseous membrane was studied in adult rats 6 h to 10 months after transection of the right sciatic nerve. Axon terminals degenerated one day after transection and were engulfed and resorbed by cells of the inner core within one week. The axial space left after removal of the axonal debris was closed by the lamellae of the inner core. The main structural features of the inner core and capsule remained preserved after denervation throughout the period of study. The denervated inner cores, however, became atrophic 10 months after neurotomy, their mean diameter being reduced by 17.5% compared with that of contralateral control corpuscles. The number of capsular lamellae was unaltered, and perineurial pathways of the peripheral nerve stump remained preserved. Schwann cells proliferated and formed Büngner bands during the first month after denervation, but retracted their processes and became atrophic at later stages after neurotomy. Survival of Pacinian corpuscles after long-term denervation in adult rats is in contrast to their rapid degeneration within several days after nerve section in neonates.
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    Close apposition of muscle cells in the longitudinal bands of the body wall of a holothurian, Isostichopus badionotus (1982)
    Springer
    Cell & tissue research 227 (1982), S. 465-473 
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    ISSN: 1432-0878
    Keywords: Electron microscopy ; Junctions ; Smooth muscle ; Echinodermata ; Holothuria, Aspidochirotida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopy reveals that sarcolemmata of adjacent muscle cells form pentalaminar junctions by fusion of apposed trilaminar double leaflet membranes. These junctions appear to be candidates for low resistance pathways between muscle fibers. The muscles depolarize slowly when bathed in solutions containing elevated concentrations of KCl, and the sucrose gap method can then be used to measure the potential difference between polarized and depolarized regions. Thus the junctions which we have observed may provide the structural basis for electrical transmission through the sucrose gap.
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    Penetration of the peritrophic membrane of the tick by Babesia microti (1982)
    Springer
    Cell & tissue research 221 (1982), S. 471-481 
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    ISSN: 1432-0878
    Keywords: Peritrophic membrane ; Tick ; Babesia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A peritrophic membrane (PM) has been demonstrated in the gut of feeding larvae, nymphs, and adults of the tick Ixodes dammini. This is the first report of a PM in ticks. This temporary structure divides the lumen of the gut into two compartments, an endoperitrophic space, the lumen proper, and an ectoperitrophic space located between the PM and the epithelial cells of the gut wall. The PM is a mechanical barrier and even such small particles as ribosomes derived from ingested reticulocytes are retained in the lumen proper; they are never found in the ectoperitrophic compartment. In Ixodes dammini fed on hamsters infected with Babesia microti some of the parasites are found in the ectoperitrophic space. This passage is accomplished by a highly specialized organelle, the arrowhead, which develops in some Babesia during their metamorphosis in the gut of the vector. The arrowhead, while passing through the PM, changes its fine structure and loses its internal organization as if releasing some of its contents. Its disintegration continues and it disappears shortly after the Babesia have entered the epithelial cells. Only Babesia equipped with the arrowhead structure are able to cross the PM. This is the first documented case of a parasite traversing a solidified PM.
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    Paddle cilia (discocilia) in chemosensitive structures of the gastropod mollusk Pleurobranchaea californica (1982)
    Springer
    Cell & tissue research 222 (1982), S. 25-40 
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    ISSN: 1432-0878
    Keywords: Paddle cilia ; Discocilia ; Pleurobranchaea ; Chemoreceptors ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scanning electron microscopy of various regions of the body of the marine gastropod Pleurobranchaea californica (McFarland) has revealed a characteristic cell type that bears cilia with dilated discoid-shaped tips. The tips of the cilia consist of an expansion of the ciliary membrane around a looped distal extension of the axoneme. These kinocilia have been observed in numerous other marine invertebrates and are generally referred to as paddle cilia (Tamarin et al. 1974) or discocilia (Heimler 1978). Although many functions have been proposed for paddle cilia, little empirical evidence supports any of the proposals. In Pleurobranchaea we have found that the distribution of this ciliated cell type corresponds exactly to areas of the body known from behavioral studies (Lee et al. 1974; Davis and Matera 1981) to mediate chemoreception. Transmission electron microscopy of the epithelium lining the rhinophores and tentacles of Pleurobranchaea revealed details of the ultrastructure of these ciliated cells and showed that they are primary receptors. These ciliated receptors lie in a yellow-brown pseudostratified columnar epithelium that superficially resembles the olfactory mucosa of vertebrates.
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    Pigment biogenesis in freshwater shrimp ventral nerve chord chromatophores (1982)
    Springer
    Cell & tissue research 222 (1982), S. 167-175 
    Details
    ISSN: 1432-0878
    Keywords: Pigment granules ; Chromatophores ; Granulogenesis ; Palaemonid shrimp ; Macrobrachium ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The possible biogenesis of two pigment granule types present in the monochromatic, brown chromatosomes enveloping the ventral nerve chord of the freshwater palaemonid shrimps Macrobrachium acanthurus, M. heterochirus and M. olfersii is examined by transmission electron microscopy in thin section and freeze fracture replicas. Prominent, membrane limited granules are suggested to have their origin in a complex, juxtanuclear, smooth endoplasmic reticulum labyrinth, continuous with the nuclear envelope. Amembranous, lipocarotenoid granules possibly derive from the external surface of the smooth endoplasmic reticulum. Nuclear envelope and SER membranes contain numerous 11 nm diameter intramembranous particles while pigment granule membranes exhibit fewer particles. A dictyosomal origin for the lipocarotenoid granules is discounted. Granulogenesis is suggested to be a continuous process in crustacean chromatophores.
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    URL: http://dx.doi.org/10.1007/BF00218297
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    Ultrastructural duality of extrafusal fibers in a slow (tonic) skeletal muscle (1982)
    Springer
    Cell & tissue research 222 (1982), S. 261-267 
    Details
    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Fiber types ; Electron microscopy ; Stereology ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural and stereological assessment of the mature avian anterior latissimus dorsi (ALD) muscle showed that it contains two kinds of extrafusal fibers. This fine structural dichotomy of fiber types in the ALD correlated well with their previously reported histochemical duality. Distinct differences occur in sarcomere banding, myofibrillar area, sarcotubular and mitochondrial density, and in morphology of motor-nerve terminals. Both myofiber types in this muscle were interpreted as representing varieties of “slow” or tonic muscle fibers. Both fibers contain myofibrils that, despite differences in cross-sectional area, were large, irregular, and ribbon-shaped, typical of the “Felderstruktur” appearance of true “slow” fibers. Whereas the majority of fibers (type-1) are devoid of well-defined M-bands, the minor fiber population (type-2) exhibit prominent M-bands in the center of each sarcomere. In addition, type-1 tonic fibers contain a significantly lower mitochondrial and sarcotubular volume than the tonic fibers of type-2. While both fiber types exhibit motor-nerve terminals that are small, smooth and punctate in appearance, those on the type2 fibers often had a number of shallow postjunctional folds. Whether or not these two classes of extrafusal fiber in this muscle represent two separate and distinct types of motor units remains to be determined functionally.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213211
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  • 42
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    Fine structure of arterial smooth muscle cells cultured in the presence of whole blood serum or plasma-derived serum (1982)
    Springer
    Cell & tissue research 223 (1982), S. 87-99 
    Details
    ISSN: 1432-0878
    Keywords: Smooth muscle cells ; In vitro-growth ; Whole blood serum ; Plasma-derived serum ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Normal diploid cells require serum to proliferate in culture. Platelet-derived growth factor has been identified as the main serum component responsible for this effect. Here, smooth muscle cells were isolated enzymatically from the aorta of 5-day-old rats and cultured in the presence of 10% whole blood serum (WBS) or plasma-derived serum (PDS), i.e. with or without platelet factor, and studied by transmission electron microscopy. The cells proliferated actively in WBS-medium but remained quiescent in PDS-medium. Fine structurally, cells from WBS-cultures demonstrated numerous mitochondria, an extensive rough endoplasmic reticulum (RER), a large Golgi complex, a few lysosomes, and microfilaments arranged in parallel bundles. After transfer to PDS-medium, the RER- and Golgi cisternae were markedly dilated and the number of membrane-associated ribosomes decreased. Segregation of fragments of cytoplasm within autophagosomes was frequently observed and the number of lysosomes increased. Lipid droplets were more abundant and often gathered in the Golgi area. Moreover, the cells had become more irregular in shape and showed many bleb-like processes at their surface. Microfilament bundles had also become more prominent and crossed each other in different directions. These observations show that the removal of platelet factor from the medium clearly modifies the fine structure of cultured smooth muscle cells. The findings are in good agreement with the concept that platelet factor not only supports the proliferation of cultured cells but also stimulates their secretory activity.
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    URL: http://dx.doi.org/10.1007/BF00221501
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    Effect of hyper- and hypoosmotic solutions on the structure of theAstacus retina (1982)
    Springer
    Cell & tissue research 223 (1982), S. 267-280 
    Details
    ISSN: 1432-0878
    Keywords: Retina (Astacus) ; Rhabdom ; Osmolarity ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Alterations of the retinula cells in the retina of the light-adapted crayfish in response to hyper- and hypoosmotic van Harreveld solutions was examined by transmission electron microscopy. Increased osmolarity of the bathing medium to twice that of the physiological value leads to shrinkage of the retinula-cell somata. Microvilli, on the other hand, do not undergo shrinkage. Some other characteristic irreversible changes do, however, take place, including detachment of microvilli from the soma, showing a two- or threefold increase in diameter, and a concomitant decrease in number, probably due to fusion of microvilli. Prolonged incubation or higher osmolarities (5 isosmol) cause alteration of the microvillar membranes to whorls. Structural changes are often restricted to microvillar stacks evaginating from certain individual retinular cells. The number of affected stacks increases depending on the duration of incubation or the osmotic pressure. Hypoosmotic solution (0.5 isosmol) also induces an increase of microvillar diameters and a concomitant reduction in number of microvilli per stack. Exposure to a 20% solution of glycerol causes destruction of the rhabdom structure and the formation of whorls from microvillar membranes. The present findings suggest that the structure of the microvilli is stabilized by an axial cytoskeleton.
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    URL: http://dx.doi.org/10.1007/BF01258488
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  • 44
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    Interdigitating cells and macrophages in the acute involuting rat thymus (1982)
    Springer
    Cell & tissue research 224 (1982), S. 291-301 
    Details
    ISSN: 1432-0878
    Keywords: Thymus (rat) irradiation ; Electron microscopy ; Interdigitating cells ; Macrophages ; Phagocytosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Phagocytic activity and population development of medullary interdigitating cells (IDC) and cortical macrophages have been studied in the rat thymus after irradiation-induced thymocyte necrosis. IDC clearly demonstrate phagocytic activity, particularly in the 16h stage after irradiation. At this stage the number of necrotic thymocytes is maximal and the total number of phagocytic cells is insufficient to phagocytize all necrotic material. IDC increase in number slightly and are predominantly phagocytizing in the corticomedullary region (CMR), where they appear to develop from macrophage-like cells. The results indicate that medullary IDC can be phagocytic but have a different developmental pathway than cortical macrophages. Cortical macrophages greatly increase in number and acquire the appearance of tingible body macrophages by phagocytizing many necrotic thymocytes. They seem to develop from monocytes that normally enter the thymus at the CMR. During the acute involution macrophages probably also enter the cortex via the connective tissue capsule. It is suggested that thymus medullary IDC probably belong to the mononuclear phagocytes, as do the cortical macrophages. In the specific medullary environment IDC gradually develop their characteristic ultrastructure for an apparently other than phagocytic function. The similarities between IDC and epidermal Langerhans cells are discussed.
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    URL: http://dx.doi.org/10.1007/BF00216874
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  • 45
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    Ultrastructural changes of the stigma of the follicle during the process of ovulation in the hen (1982)
    Springer
    Cell & tissue research 224 (1982), S. 349-359 
    Details
    ISSN: 1432-0878
    Keywords: Ovarian follicle ; Ovulation ; Ultrastructural change ; Fowl ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary No structural changes could be observed in the stigma until 2 h before ovulation. Within a few minutes of ovulation, the stratum granulosum and theca interna disappear completely in the middle part of the stigma. By 30 min before ovulation, the rough endoplasmic reticulum in the fibroblasts of the theca externa develops conspicuously. Then a few minutes before ovulation, the theca externa becomes much thinner with a conspicuous disintegration of the collagenous fibers into individual fibrils. These structural changes may contribute to the fragility of the stigma tissue and induce rupture of the follicle.
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    URL: http://dx.doi.org/10.1007/BF00216878
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  • 46
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    Cellular interconnections in the young mouse ovary (1982)
    Springer
    Cell & tissue research 224 (1982), S. 383-395 
    Details
    ISSN: 1432-0878
    Keywords: Mouse ovary ; Junctions ; Freeze-fracture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intercellular junctions in the young mouse ovary were examined by electron microscopy utilizing freeze-fracture and thin-sectioning techniques. Projections from the granulosa cells adjacent to the oocyte (GI) traverse the zona pellucida and form small gap junctions on the oocyte surface. On the P-face of these cells, the junctional aggregations are occasionally associated with linear strands of particles. In contrast, large gap junctional areas are frequently observed between the more peripherally located granulosa cells (GE) and are also present in the theca interna (TI) cell layer surrounding the follicles. Three types of tight junctional strands are discernible on the P-face of theca externa cells (TE): angularly zigzag strands consisting of intermittently distributed intramembranous particles on wide ridges, intermediate zigzag strands consisting of more continuously distributed particles, and wavy strands consisting of rather fused particles. Tight junctional strands are also present in the middle of grooves on the E-face of endothelial cells of blood vessels. In the germinal epithelial cell layer, tight junctional strands appear to be discrete and form a less anastomosing network.
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    URL: http://dx.doi.org/10.1007/BF00216881
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  • 47
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    Ultrastructure of the pars intermedia of the developing sheep hypophysis (1982)
    Springer
    Cell & tissue research 224 (1982), S. 369-381 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Pars intermedia ; Electron microscopy ; Fetal sheep ; Lamb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using light and electron microscopy, the morphogenesis of the pars intermedia of the sheep pituitary gland was examined in developing lambs between 26 days of gestation and the newborn stage. Following the establishment of contact between the anterior and posterior lobe primordia seen at 26 days, the connection with the pharyngeal roof disappeared by 31 days. The lumen of Rathke's pouch, which was a prominent cavity at the earlier stages, became inconspicuous by 40 days but progressively increased in size during gestation and, in some newborn animals, contained colloid material. At 40 days, the pars intermedia consisted of a uniform population of undifferentiated cells. Cells with cytoplasmic granules were first identified at 50 days. The cytological appearance of granular cells at 70 days indicated increased synthetic activity and by 80 days they closely resembled adult glandular cells. At 100 days, membrane activity suggestive of exocytosis was first observed in granular cells; fenestrated capillaries were present, and early follicle formation between adjacent non-granular cells was seen. This apparent exocytotic release of granules was observed much more frequently between 100 days of gestation and the newborn stage than in adult pars intermedia cells. These findings indicate that glandular cells of the developing pars intermedia are actively engaged in synthesis, storage and secretion from an early stage.
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    URL: http://dx.doi.org/10.1007/BF00216880
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  • 48
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    Endometrial ultrastructure in the early uterine response to blastocysts and artificial deciduogenic stimuli in rats (1982)
    Springer
    Cell & tissue research 225 (1982), S. 355-364 
    Details
    ISSN: 1432-0878
    Keywords: Implantation ; Blastocyst ; Pontamine ; blue reaction ; Decidualization ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The early uterine response to transplanted, delayed and estrogenactivated blastocysts was studied ultrastructurally and compared with that induced by intrauterine instillations of deciduogenic agents (arachis oil, air). The uterine responses to delayed and activated blastocysts showed no ultrastructural or temporal differences. Already within 4 h after transfer to a sensitized uterus, the delayed blastocysts exhibited signs of activation, and both types of blastocysts had started to attach onto an undamaged epithelial lining. Signs of stromal cell differentiation into decidual cells were also seen as early as 4 h after transfer, while the Pontamine-blue reaction did not appear until after 8 h. The results therefore indicate that the transplanted blastocysts induced decidualization atraumatically and that the delayed blastocysts were either deciduogenic already before transfer or rapidly acquired deciduogenic properties after transfer. Artificial decidual induction with oil and air led to damage or death of a large number of cells in the uterine luminal epithelium. Within only 15 min after instillation pronounced signs of cell damage were seen, and later numerous cells were extruded from the epithelial lining. In the stroma ultrastructural signs of decidual cell differentiation and a Pontamine-blue reaction were observed as early as 4 h after induction. It is therefore suggested that oil and air induce decidualization via the epithelium by means of trauma.
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    URL: http://dx.doi.org/10.1007/BF00214688
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    Ultrastructural studies on the ventricular surface of the frog cerebellum (1982)
    Springer
    Cell & tissue research 225 (1982), S. 443-448 
    Details
    ISSN: 1432-0878
    Keywords: Frog cerebellum ; Ependymal surface ; Cilia ; Supraependymal cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural studies of the ventricular surface of the frog cerebellum showed regional differences. In the midline region of the adult cerebellum was found a band of profusely ciliated squamous ependymal cells. In the rest of the cerebellum the ependymal cells were columnar and each had a single cilium. In the cerebellum of the premetamorphic tadpole, the squamous ependymal cells of the midline region also were monociliated. During metamorphosis they gradually became multiciliated. Additionally, supraependymal cells and synaptic elements were present on the ventricular surface of the cerebellum of adult frogs as well as in late metamorphic tadpoles. In contrast, supraependymal cells were rarely observed in premetamorphic tadpoles, and it was concluded that the supraependymal system develops during metamorphosis. It is postulated that the band of cilia may be associated with the circulation of cerebrospinal fluid, and supraependymal synaptic elements function in neuroendocrine regulation.
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    URL: http://dx.doi.org/10.1007/BF00214695
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  • 50
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    Further studies on the junctional complex in the intestine of Sagitta setosa (1982)
    Springer
    Cell & tissue research 225 (1982), S. 663-671 
    Details
    ISSN: 1432-0878
    Keywords: Chaetognatha ; Intestine ; Pleated septate junction ; Electron microscopy ; Freeze-fracture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The intramembrane structures of the pleated septate junction which occur in the junctional complex of the intestine of the chaetognath Sagitta setosa have been investigated. The pleated septate junction is made up of linear rows of irregularly shaped and sized particles, often fused into short rods, and pits which can be fused into furrows. The distribution of these structures on E and P faces depends upon the preparative methods used. Many of the morphological characteristics are the same as those of the “lower invertebrate pleated septate junction type” defined by Green (1981a). The physiological significance of this junction is obscure. On the basis of the presence of septate junctions (both of the paired septate junction and pleated septate junction types) which have mainly morphological characteristics of the “lower invertebrate pleated septate junction” we can add to the hypothesis that chaetognaths are not related to the molluscs and arthropods.
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    URL: http://dx.doi.org/10.1007/BF00214811
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    An immunocytochemical and electron-microscopical study of endocrine cells in the gut and pancreas of a stomachless teleost fish, Barbus conchonius (Cyprinidae) (1982)
    Springer
    Cell & tissue research 227 (1982), S. 577-593 
    Details
    ISSN: 1432-0878
    Keywords: Enteroendocrine cells ; Pancreatic endocrine cells ; Gastroenteropancreatic hormones ; Immunocytochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four immunoreactive endocrine cell types can be distinguished in the pancreatic islets of B. conchonius: insulin-producing B cells, somatostatin-producing A1 (= D) cells, glucagon-producing A2 cells and pancreatic poly-peptide-producing PP cells. The principal islet of this species contains only a few PP cells, while many PP cells are present in the smaller islets. Except for the B cell all pancreatic endocrine cell types are also present in the pancreatic duct. At least six enteroendocrine cell types are present in the gut of B. conchonius: 1. a cell type (I) with small secretory granules, present throughout the intestine, and possibly involved in the regulation of gut motility; 2. a C-terminal gastrin immunoreactive cell, probably producing a caerulein-like peptide; these cells are located at the upper parts of the folds, especially in the proximal part of the intestinal bulb; 3. a met-enkephalin-immunoreactive cell, present throughout the first segment; 4. a glucagon-immunoreactive cell, which is rare in the first segment; 5. a PP-immunoreactive cell, mainly present in the first half of the first segment; 6. an immunoreactive cell, which cannot at present be specified, located in the intestinal bulb. The latter four cell types are mostly located in the basal parts of the folds, although some PP-immunoreactive cells can also be found in the upper parts. Most if not all enteroendocrine cells are of the open type. The possible functions of all enteroendocrine cell types are discussed.
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    URL: http://dx.doi.org/10.1007/BF00204788
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    Splenic white pulp as a thymus-independent area in the African clawed toad, Xenopus laevis (1982)
    Springer
    Cell & tissue research 226 (1982), S. 327-335 
    Details
    ISSN: 1432-0878
    Keywords: Xenopus ; Spleen ; B-lymphocyte ; Immunofluorescence ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An indirect immunofluorescence study of the frozen sections of the spleen of an anuran amphibian, Xenopus laevis, showed that lymphocytes bearing a small amount of immunoglobulin (Ig) were localized mostly in the white pulp of non-immunized toads. There were fewer fluorescent cells in the red pulp. In the toads hyperimmunized with human gamma globulin (HGG), cells with strong cytoplasmic fluorescence increased significantly in the outer part of the white pulp. Electron microscopy of spleens from these toads showed that plasma cells at different stages of maturation were abundant in the white pulp, whereas in the red pulp, a smaller number of maturer plasma cells were observed. These results indicate that, in contrast with its mammalian counterpart, the splenic white pulp of this anuran is the site where thymusin-dependent lymphocytes commence blast formation and transformation into plasma cells.
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    URL: http://dx.doi.org/10.1007/BF00218363
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