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  • photosynthesis  (88)
  • Angiosperms  (79)
  • Springer  (167)
  • American Meteorological Society
  • 1995-1999  (48)
  • 1990-1994  (119)
  • 1940-1944
  • 1998  (48)
  • 1990  (119)
  • 1941
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  • Springer  (167)
  • American Meteorological Society
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  • 1995-1999  (48)
  • 1990-1994  (119)
  • 1940-1944
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  • 1
    Electronic Resource
    Electronic Resource
    Field measurements of gas exchange in Avicennia marina and Bruguiera gymnorrhiza (1998)
    Springer
    Mangroves and salt marshes 2 (1998), S. 99-107 
    Details
    ISSN: 1572-977X
    Keywords: conductance ; mangrove ; photosynthesis ; productivity ; water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diurnal gas exchange characteristics were measured simultaneously in two mangrove species, Avicennia marina and Bruguiera gymnorrhiza, over 7 d in summer (February–March), to compare their productivity. The study was undertaken in the Beachwood Mangroves Nature Reserve, Durban, South Africa, using fully expanded leaves of young and mature trees at the top of the canopy. Gas exchange was strongly influenced by photosynthetic photon flux density (PPFD), leaf temperature and the accompanying leaf to air vapour pressure deficit (Δ w). Carbon dioxide exchange was saturated at a PPFD of about 600 μmol m-2s-1 in B. gymnorrhiza compared to 800 μmol m-2s-1 in A. marina. Maximal CO2 exchange occurred between 12h00 and 14h00 and was consistently greater in A. marina (8.8 μmol m-2s-1) than in B. gymnorrhiza (5.3 mu;mol m-2s-1). Mean internal CO2 concentrations ( ci) were 260 μl l-1 in A. marina and 252 μl l-1 in B. gymnorrhiza. Photorespiratory activity was 32% in A. marina and 30% in B. gymnorrhiza. Mean water use efficiency (WUE) was 8.0 μmol mmol-1 in A. marina and 10.6 μmol mmol-1 in B. gymnorrhiza. Diurnal leaf water potentials ranged from –0.8 to –3.5 MPa and were generally lower in A. marina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009939523164
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  • 2
    Electronic Resource
    Electronic Resource
    Mangrove forest productivity and biomass accumulation in Hinchinbrook Channel, Australia (1998)
    Springer
    Mangroves and salt marshes 2 (1998), S. 191-198 
    Details
    ISSN: 1572-977X
    Keywords: canopy ; Hinchinbrook ; leaf area index ; mangrove ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data on stand structure and rates of photosynthesis were used to estimate net canopy carbon fixation and carbon accumulation as living biomass in mangrove forests in Hinchinbrook Channel, Australia. Total annual canopy net carbon fixation was estimated to be about 29 t C ha−1 yr−1. This equates to about 204,000 t C yr−1 for all mangrove forests in Hinchinbrook Channel. Of this, only about 12% was stored as living plant biomass. Although it is not yet possible to present a robust carbon balance for mangrove trees, the remainder is presumably lost through plant respiration, litter fall, root turnover and exudation of organic compounds from roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009979610871
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation of the male germ unit: organization and function in tobacco (Nicotiana tabacum L.) (1998)
    Springer
    Plant cell reports 18 (1998), S. 143-147 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsNicotiana tabacum ; Male germ unit ; Scanning electron microscopy ; Sperm isolation ; Angiosperms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sperm cells are released from pollen tubes of tobacco as linked cells, associated with the vegetative nucleus in an assemblage known as the male germ unit (MGU). Using light microscopy, the MGU assemblage appears to be ensheathed by cytoplasmic material of the pollen tube, which may stabilize their association. Following their release, the shape of the sperm cells and vegetative nucleus changes from an ellipsoidal to a more spheroidal morphology. When most of the cytoplasmic material is dispersed, a boundary remains around the two sperm cells. Using scanning electron microscopy, the cytoplasmic material surrounding the MGU appears filamentous, sometimes twisted and rope-like. Based on these observations, the function of the MGU of tobacco is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050547
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  • 4
    Electronic Resource
    Electronic Resource
    Ground cover, photosynthetic rate and tuber yield of potato (Solanum tuberosum L.) crops from seed tubers with different physiological age modified by foliar applications of plant growth regulators (1998)
    Springer
    Potato research 41 (1998), S. 175-185 
    Details
    ISSN: 1871-4528
    Keywords: benzylaminopurine ; gibberellic acid ; senescence ; photosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The modifying effects of applying the plant growth regulators (PGRs) benzylaminopurine (BAP), gibberellic acid (GA3) and BAP+GA3 on physiological age were studied. Two experiments with two cultivars, differing in rate of physiological ageing (medium-early Pampeana, medium-late Huinkul) and two storage systems were performed during 1988/89 and 1989/90 in two different potato areas of Argentina. In both seasons seed tubers stored in heaps reached an advanced physiological age at planting, compared with tubers from the cold store. Seed tubers of cv. Pampeana were older than those of Huinkul. compared with control crops, those sprayed with BAP maintained ground cover and photosynthesis for longer, and those sprayed with GA3 for a shorter period. Consequently tuber yield was decreased by GA3 in 1988/89, but in 1989/90 all crops treated with PGRs outyielded the control. BAP could overcome effects of advanced physiological age on crop senescence and tuber yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358440
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  • 5
    Electronic Resource
    Electronic Resource
    Asphodelus tenuifolius andA. fistulosus (Liliaceae) are morphologically, genetically, and biologically different species (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 1-12 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Liliaceae ; Asphodelus tenuifolius ; A. fistulosus ; Cytogenetics ; electrophoretics ; morphology ; duplication genes ; speciation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biological analysis of six populations ofAsphodelus tenuifolius and 12 populations ofA. fistulosus has confirmed that they are separate species. Both their floral structures (length of the tepals, stamens, anthers and style) and also their pollen size are clearly different.A. tenuifolius has only the 2n = 28 chromosome race, whileA. fistulosus has 2n = 28 and 2n = 56.A. tenuifolius is genetically less variable thanA. fistulosus and they have different electrophoretic mobilities. Gene duplication phenomena exist in the 2n = 28 level of both species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935979
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  • 6
    Electronic Resource
    Electronic Resource
    Allozyme and life history variation in five northwardly colonizing North American weed species (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 41-54 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Abutilon theophrasti ; Datura stramonium ; Panicum miliaceum ; Sorghum halepense ; Setaria faberi ; Weeds ; allozymes ; life history ; variation ; Flora of N. America
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationships of allozyme and life history variation in a particularly narrow ecological setting are studied. Levels of genetic variation are compared in five introduced, predominantly selfing weedy species that are undergoing rapid range expansion northward in eastern N. America, mostly in monocultures of soybean and maize. In all of these species, a low level of allozyme variation contrasts sharply with the substantial inter- and intrapopulational variation in morphological and phenological life history features. Evolutionary and historical factors, determining variability of the species examined are reviewed, including founder effects, breeding system, environmental homogeneity, polyploidy, domestication, and crop-weed interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935983
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  • 7
    Electronic Resource
    Electronic Resource
    Chemo- and karyotaxonomic studies on some rhizomatous species of the genusSymphytum (Boraginaceae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 31-39 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Boraginaceae ; Symphytum tuberosum complex ; S. grandiflorum agg ; Chemotaxonomy ; pyrrolizidine alkaloids ; triterpenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract InS. tuberosum subspp.tuberosum andnodosum, S. grandiflorum andS. ibericum the presence of the pyrrolizidine alkaloids lycopsamine, echimidine and symphytine could be demonstrated. The taxonS. tuberosum contains an unknown compound that seems to be specific for this taxon. This compound is not the pyrrolizidine alkaloid anadoline which has previously been reported for this species. It is possibly represented by a peak on GC/MS with a molecular ion peak at m/z 623 (as TMS derivative) and can be used as a chemotaxonomic marker for the speciesS. tuberosum. The pyrrolizidine alkaloid pattern of the two subspecies ofS. tuberosum reinforces the close relationship. Fresh material ofS. tuberosum contained the triterpene isobauerenol, but in herbarium material isobauerenol was lacking. InS. grandiflorum, neither fresh nor dried material contains isobauerenol. In herbarium material ofS. ibericum also no isobauerenol could be found. More extensive chemotaxonomical research is necessary to support the view thatS. abchasicum is more closely related toS. ibericum than toS. grandiflorum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935982
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  • 8
    Electronic Resource
    Electronic Resource
    Investigations into the evolutionary history of theAntennaria rosea (Asteraceae: Inuleae) polyploid complex (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 97-110 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Compositae ; Inuleae ; Antennaria ; A. rosea ; Agamic complex ; agamospermy ; asexual reproduction ; clonal organism ; dioecy ; morphology ; numerical taxonomy ; phenetics ; polyploidy ; systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheAntennaria rosea polyploid agamic complex is one of the most morphologically diverse and widespread complexes of N. AmericanAntennaria. The group is taxonomically confusing because of numerous agamospermous microspecies, having been recognized as distinct species. Morphometric analyses have demonstrated that the primary source of morphological variability in the complex derives from six sexually reproducing progenitors,A. aromatica, A. corymbosa, A. media, A. microphylla, A. racemona, andA. umbrinella. Additionally, two other sexually reproducing species,A. marginata andA. rosulata, may have contributed to the genetic complexity of theA. rosea complex. Cluster analysis indicates that four discrete morphological groups exist within theA. rosea complex. Each group could be the result of predominance of genes from different groups of sexual progenitors. AsA. rosea is of multiple hybrid origin, from among several sexual progenitors, it is advisable to recognizeA. rosea as a distinct species from its sexual progenitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935988
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  • 9
    Electronic Resource
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    Patterns of intraspecific variation inAntennaria alborosea, A. corymbosa, A. marginata, A. microphylla, A. parvifolia, andA. umbrinella (Asteraceae:Inuleae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 123-150 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Inuleae ; Antennaria ; Geographic variation ; clinal variation ; principal component analysis ; correlation coefficients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Patterns of intraspecific variation were examined inAntennaria alborosea A. E. Porsild,A. corymbosa E. Nels,A. marginata Greene,A. microphylla Rydb.,A. parvifolia Nutt., andA. umbrinella Rydb. AlthoughA. alborosea was initially considered arctic in distribution, it became apparent that a southern montane element also exists. Our results suggest that morphological differences between arctic and southern montane specimens represent clinal variation. The additional morphological data for specimens that occur more than 1,500 km south of the species' range as it was initially described result in a better understanding of this once presumed arctic taxon. Morphological variation in the dioecious speciesA. corymbosa, A. marginata, A. microphylla, A. parvifolia, andA. umbrinella was greater between the genders than was geographic variation within each gender. These results demonstrate that both pistillate and staminate specimens must be examined in dioecious species ofAntennaria if morphological variation in the respective species is to be fully understood. Character size or number of broadly distributed species (A. microphylla andA. parvifolia) generally decreased with increasing longitude, whereas characters of species with more restricted distributions (A. alborosea, A. corymbosa, andA. marginata) generally increased in size or number with increasing latitude or longitude.Antennaria umbrinella was an exception in this respect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937672
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  • 10
    Electronic Resource
    Electronic Resource
    Chromosome number of theOxalis tuberosa alliance (Oxalidaceae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 25-29 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Oxalidaceae ; Oxalis tuberosa alliance ; Chromosome numbers ; karyotype analyses ; cytotaxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twelve taxa of theOxalis tuberosa alliance were analysed and found to share the same basic chromosome number x = 8. The karyotypes are composed by small metacentric and submetacentric chromosomes. Different ploidy levels were found among the taxa: there were 9 diploids, 1 tetraploid, 1 hexaploid and 1 octoploid. The last ploidy level corresponds toO. tuberosa, the only tuber bearing taxon found so far in the alliance. Cytotaxonomic evidence and evolutionary considerations suggest to classify theO. tuberosa alliance in sect.Herrerea.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935981
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  • 11
    Electronic Resource
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    Buzz-pollination in three nectariferousBoraginaceae and possible evolution of buzz-pollinated flowers (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 65-68 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Boraginaceae ; Onosma gigantea ; Trichodesma africana ; Trichodesma boissieri ; Buzz-pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Buzz-pollination was observed in three nectariferousBoraginaceae spp.:Onosma gigantea Lam.,Trichodesma africana (L.)R. Br. andT. boissieri Post. An evolutionary pathway from usual nectariferous flowers to typical buzz-pollinated flowers is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935985
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  • 12
    Electronic Resource
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    Phylogenetic relationships and gene flow between sympatric diploid and tetraploid plants ofDactylis glomerata (Gramineae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 81-96 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Gramineae ; Festuceae ; Dactylis glomerata ; Enzymatic markers ; phenology ; interploid exchanges ; autopolyploidy ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phylogenetic relationships between sympatric, morphologically indistinguishable diploid and tetraploid plants ofDactylis glomerata L. (Gramineae) in Galicia (Spain) were assessed using allozyme markers for 6 distinct systems. The study exploited recent introduction in Galicia and subsequent hybridization of an alien 4xDactylis subspecies possessing distinct allozymes from those of all the native plants. Opportunities for gene exchanges between the ploidies were estimated from in situ observations of flowering, examination of progenies in 2x/4x natural and experimental crosses, and enzyme analyses. Results show a high genetic similarity between the Galician diploids and tetraploids, which possess peculiar alleles in common. Although the ploidy levels usually have distinct flowering periods, interploidal crosses do occasionally occur. Gene flow is likely much more important from the diploid to the tetraploid level. A good genetic intermixing occurs between the Galician and the alien tetraploid entities which have simultaneous flowering. Autopolyploidization of the diploids followed by various rates of hybridization is proposed as one very probable origin of natural tetraploids inDactylis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00935987
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  • 13
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    The genusAntennaria (Asteraceae: Inuleae) in western North America: Morphometric analysis ofAntennaria alborosea, A. corymbosa, A. marginata, A. microphylla, A. parvifolia, A. rosea, andA. umbrinella (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 151-175 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Inuleae ; Antennaria alborosea ; A. corymbosa ; A. marginata ; A. microphylla ; A. parvifolia ; A. rosea ; A. umbrinella ; Taxonomy ; multivariate morphometrics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Multivariate analysis of vegetative and reproductive characters was used to examine morphological relatedness amongAntennaria alborosea A. E. Porsild,A. corymbosa E. Nels.,A. marginata Greene,A. microphylla Rydb.,A. parvifolia Nutt.,A. rosea Greene, andA. umbrinella Rydb. Both pistillate and staminate plants were examined. Some of the characters examined were variable in one species, but stable in another (i.e., presence or absence of papillae on the achenes). Our analyses indicate that the seven species are morphologically distinct. It is hypothesized that theA. rosea agamic complex arose through hybridization amongA. corymbosa, A. microphylla, A. umbrinella, and possiblyA. dioica (L.)Gaertn. However, hybridization between the three former species and others, as well as their subsequent morphological responses to different environmental conditions causes confusion in recognizing the taxa.Antennaria angustifolia Rydb.,A. arida E. Nels.,A. confinis Greene,A. scariosa E. Nels.,A. foliacea humilis Rydb.,A. concinna E. Nels., andA. viscidula E. Nels. are considered to represent F 1 hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937673
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  • 14
    Electronic Resource
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    Transfer cells and lipid droplets of someValerianaceae (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 37-51 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Valerianaceae ; Transfer cells ; lignification ; lipid droplets ; oil cells ; tannins ; Development ; structure ; axial distribution ; transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development, structure and the axial distribution of transfer cells and their lignification were investigated inValerianella locusta, Valeriana officinalis, andV. tuberosa (Valerianaceae). Fundamental new results are: (1) Transfer cells often contain numerous lipid droplets. Within the stem the distribution of cells containing lipid droplets correlates to that of transfer cells. (2) InValeriana officinalis persisting protuberances are frequently found on pit membranes of xylem transfer cells. Lignified transfer cells can undergo a second modification: a layer covering the secondary wall forms wall ingrowths similar to those of transfer cells. (3) Peripheral pith cells, abuting transfer cells, are able to modify into transfer cells. Cambial derivatives are only temporarily developed as transfer cells. (4) Phloem transfer cells are found in vascular bundles of the whole axis. (5) In roots, xylem transfer cells are poorly developed or absent. (6) Oil cells with oil bodies are present in the rape ofValeriana tuberosa. They are absent however in the stem of the species investigated. (7) Tannins occur in elements of the primary cortex, phloem and secondary xylem ofValeriana officinalis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937848
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  • 15
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    Invasive tapetum and tricelled pollen inAmbrosia trifida (Asteraceae, tribeHeliantheae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 237-243 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Heliantheae ; Ambrosia ; Anther ; crystals ; pollen ; sperm cells ; tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Staminate flowers of giant ragweed,Ambrosia trifida L. (Asteraceae, tribeHeliantheae, subtribeAmbrosiinae) were processed into resin and sectioned 1–2 µm thick. The invasive (amoeboid) anther tapetum remains parietal until microspores are released from tetrads, then it swells and invades the locule, merging gradually into a single protoplast that flows among the microspores. After the tapetal membrane ruptures at late microspore stage, tapetal debris fills the locule, then disappears as pollen matures. Pollen becomes tricelled before anthesis. The two sperm cell nuclei are slender and wormlike. The present report supports the two generalizations that invasive tapetum and tricelled pollen are attributes of theAsteraceae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937677
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  • 16
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    Breeding systems in thePolemoniaceae (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 205-214 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Polemoniaceae ; Pollen ; ovule ratio ; stigmatic pollen germination ; breeding systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pollen — ovule ratios and percentages of stigmatic pollen germination (SPG) were measured for over 160 taxa of thePolemoniaceae. When related to taxa with known breeding systems, it is found that low SPGs and high P:O ratios characterize xenogamous plants, high SPGs and low P:Os characterize autogamous plants. There is a significant negative correlation between P:O ratio and SPG in the whole family as well as in certain genera. Both measures can serve as reliable indicators of the breeding systems in taxa of thePolemoniaceae and can be measured in dried or living specimens. Accordingly, most polemons feature “mixed” breeding system, i.e. facultative xenogamy or facultative autogamy. Xenogamy is common among the tropical genera and in theLeptodactylon, Phlox andPolemonium. Autogamy is more frequent in the tribeGilieae (particularly inGilia) than in the other tribes. Annual taxa tend to be autogamous, showing on the average higher SPG and lower P:O ratio. The lepidopteran-pollinated group of species have a higher mean P:O ratio and lower mean SPG thus indicating that such plants are associated with crosspollination more than the others.
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    URL: http://dx.doi.org/10.1007/BF00937704
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  • 17
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    Numerical analyses of karyotypic diversity in the genusEupatorium (Compositae, Eupatorieae) (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 215-228 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Eupatorium ; Chromosomal distance ; chromosomal identity ; karyotype analysis ; statistics phenetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic metaphase karyotypes were analyzed for 22 diploid species ofEupatorium. The karyotypic comparisons were made using two indices: minimal chromosomal distance (MCD), measuring overall dissimilarities, and chromosomal identity (CI), measuring number of morphologically identical chromosomes between species. The resulting phenograms from these indices are largely compatible. The 22 species cluster into four groups in the phenogram using MCD, and the grouping corresponds well with morphology or geographic distribution into the three N. American groupsEutrochium, Uncasia, Traganthes, and the E. Asian group. These results suggest that karyotypes in perennialEupatorium have been considerably conservative and changed not through large chromosomal mutations but through small chromosomal mutations gradually fixed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00937705
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  • 18
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    A study of genetic diversity among host-specific populations of the witchweedStriga hermonthica (Scrophulariaceae) in Africa (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 1-12 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Scrophulariaceae ; Striga hermonthica ; Hemiparasitism ; hostspecificity ; enzyme electrophoresis ; genetic diversity ; Flora of Burkina Faso ; Sudan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Striga hermonthica is a root hemiparasite that attacks onlyGramineae, includingSorghum and millet for which it is a principal cause of lowered yield. Enzyme electrophoresis was used to investigate genetic diversity inStriga hermonthica and to determine the level of differentiation between host-specialized populations. Nine genetic loci coding eight enzymes were interpreted and data obtained from three populations: oneSorghum-adapted population from Sudan and two populations from Burkina Faso, oneSorghum-adapted and the other millet-adapted. Levels of polymorphism were similarly high in all three populations (P=0.625, A=2.6−2.8, H=0.293−0.401). Genotypic frequencies at most loci conformed to Hardy-Weinberg expectations in each population, consistent with outcrossing as predicted from previous studies of floral biology. Occasional heterozygote deficiencies were probably the result of Wahlund effect. The mean value of FST over the three populations was 0.068, indicating a slight to moderate level of genetic differentiation among the populations. The two Burkina Faso populations were more closely related (S=0.940, D=0.006) than either was to the Sudan population, suggesting that geographic separation is more important than host specialization in contributing to population differentiation. TheSorghum-adapted population was slightly closer to the Burkina FasoSorghum-adapted population (S=0.873, D=0.047) than to Burkina Faso millet-adapted population (S=0.851, D=0.074). The absence of substantial genetic divergence between host-specific populations ofStriga could result either from recent evolution of host-specialized strains or from strong selection for physiological specialization in the face of substantial gene flow between the populations.
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    URL: http://dx.doi.org/10.1007/BF00937794
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  • 19
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    Effect of thiobencarb on growth and photosynthesis of the soil alga Protosiphon botryoides (Chlorophyta) (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 547-554 
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    ISSN: 1573-5176
    Keywords: herbicide ; green alga ; growth ; nutrients ; photosynthesis ; it Protosiphon botryoides ; respiration ; Thiobencarb
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of the herbicide thiobencarb (Saturn) were tested on the growth and physiology of the chlorophyte Protosiphon botryoides isolated from an Egyptian paddy. Assays were conducted using 16-day batch cultures. Chlorophyll and dry weight biomass yields were significantly reduced at 2–3 mg L-1 thiobencarb, and dark respiration increased and protein decreased significantly at 3 mg L-1. Reductions in exponential specific growth rate (μ) were generally small, but in some cases significant. Thiobencarb also slightly, but significantly, reduced the 77 K fluorescence parameter Fv/Fm, an indicator of maximum photosynthetic efficiency. No consistent dose-dependent changes occurred in chlorophyll per unit dry weight, total carbohydrate or gross photosynthetic capacity. Whereas half of the added thiobencarb was recovered from control (uninoculated) medium, it was largely absent from cells and culture medium after sixteen days, indicating biodegradation by the alga or associated bacteria. P. botryoides recovered fully within sixteen days following subculture in thiobencarb-free medium. Independently varying phosphate and nitrate nine-fold had no clear effect on the sensitivity of P. botryoides to thiobencarb.
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    URL: http://dx.doi.org/10.1023/A:1008092826338
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    Effects of increased atmospheric CO2 and N supply on photosynthesis, growth and cell composition of the cyanobacterium Spirulina platensis (Arthrospira) (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 461-469 
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    ISSN: 1573-5176
    Keywords: Cyanobacterium ; Spirulina platensis ; Arthrospira ; CO2 ; organic carbon ; nitrogen ; photosynthesis ; batch culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The consequences of the addition of CO2 (1%) in cultures of S. platensis are examined in terms of biomass yield, cell composition and external medium composition. CO2 enrichment was tested under nitrogen saturating and nitrogen limiting conditions. Increasing CO2 levels did not cause any change in maximum growth rate while it decreased maximum biomass yield. Protein and pigments were decreased and carbohydrate increased by high CO2, but the capability to store carbohydrates was saturated. C:N ratio remained unchanged while organic carbon released to the external medium was enhanced, suggesting that organic carbon release in S. platensis is an efficient mechanism for the maintenance of the metabolic integrity, balancing the cell C:N ratio in response to environmental CO2 changes. CO2 affected the pigment content: Phycocyanin, chlorophyll and carotenoids were reduced in around 50%, but the photosynthetic parameters were slightly changed. We propose that in S. platensis CO2 could act promoting degradation of pigments synthetised in excess in normal CO2 conditions, that are not necessary for light harvesting. Nitrogen assimilation was significantly not affected by CO2, and it is proposed that the inability to stimulate N assimilation by CO2 enrichment determined the lack of response in maximum growth rate.
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    URL: http://dx.doi.org/10.1023/A:1008090402847
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    Response of the tropical red seaweed Gracilaria cornea to temperature, salinity and irradiance (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 419-425 
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    ISSN: 1573-5176
    Keywords: Gracilaria cornea ; photosynthesis ; respiration ; chlorophyll ; phycoerythrin ; Florida ; salinity ; temperature ; irradiance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The agarophyte Gracilaria cornea, collected over 2.5 y in the Florida Keys, shows adaptations to oceanic salinities and subtropical to tropical water temperatures in its photosynthetic and respiratory responses as measured with a respirometer. No seasonal pattern in responses to irradiance, temperature, and salinity were evident between five collections over a 20-month period, indicating the tropical nature of the populations from Bahia Honda and Pigeon Keys. Concentrations of chlorophyll a (0.09 to 0.41 mg g d wt-1) and phycoerythrin (0.06 to 0.36 mg g d wt- 1) were low and reflect the low nutrient regime of the habitats, especially when compared to laboratory cultured plants. Compensation and saturation irradiances were also low (11–38 and 90–127 μmol photon m-2 s-1), indicating acclimation to lower irradiances in their shallow (1–2 m depth) habitats where turbidity can be high. In comparison with other subtropical and warm temperate species of Gracilaria, G. cornea had lower levels of pigment, but similarly high photosynthetic efficiency, demonstrating shade adaptation; it had only limited tolerance to salinities below 20‰ and temperatures below 15 °C. Thus, G. cornea from the Florida Keys in mariculture would require subtropical to tropical temperatures and stable oceanic salinities.
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    URL: http://dx.doi.org/10.1023/A:1008021613399
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    Dunaliella salina (Chlorophyta) with small chlorophyll antenna sizes exhibit higher photosynthetic productivities and photon use efficiencies than normally pigmented cells (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 515-525 
    Details
    ISSN: 1573-5176
    Keywords: Chlorophyll antenna size ; damage and repair cycle ; photon use efficiency ; photosynthesis ; photoinhibition ; Dunaliella salina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The photon use efficiencies and maximal rates of photosynthesis in Dunaliella salina (Chlorophyta) cultures acclimated to different light intensities were investigated. Batch cultures were grown to the mid-exponential phase under continuous low-light (LL: 100 μmol photon m-2 s-1) or high-light (HL: 2000 μmol photon m-2 s-1) conditions. Under LL, cells were normally pigmented (deep green) containing ∼500 chlorophyll (Chl) molecules per photosystem II (PSII) unit and ∼250 Chl molecules per photosystem I (PSI). HL-grown cells were yellow-green, contained only 60 Chl per PSII and 100 Chl per PSI and showed signs of chronic photoinhibition, i.e., accumulation of photodamaged PSII reaction centers in the chloroplast thylakoids. In LL-grown cells, photosynthesis saturated at ∼200 μmol photon m-2 s-1 with a rate (Pmax) of ∼100 mmol O2 (mol Chl)-1 s-1. In HL-grown cells, photosynthesis saturated at much higher light intensities, i.e. ∼2500 μmol photon m-2 s-1, and exhibited a three-fold higher Pmax (∼300 mmol O2 (mol Chl)-1 s-1) than the normally pigmented LL-grown cells. Recovery of the HL-grown cells from photoinhibition, occurring prior to a light-harvesting Chl antenna size increase, enhanced Pmax to ∼675 mmol O2 (mol Chl)-1 s-1. Extrapolation of these results to outdoor mass culture conditions suggested that algal strains with small Chl antenna size could exhibit 2–3 times higher productivities than currently achieved with normally pigmented cells.
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    URL: http://dx.doi.org/10.1023/A:1008076231267
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    Light dependency of the photosynthetic recovery of Nostoc flagelliforme (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 51-53 
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    ISSN: 1573-5176
    Keywords: blue-green alga ; cyanobacterium ; Fv/Fmlight ; Nostoc flagelliforme ; photosynthesis ; rewetting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract PS II photochemical efficiency (Fv/Fm) of Nostoc flagelliforme was examined after rewetting in order to investigate the light-dependency of its photosynthetic recovery. Fv/Fm was not detected in the dark, but was immediately recognized in the light. Different levels of light irradiation (4, 40 and 400 µmol photon m2 s-1) displayed different effects on the recovery process of photosynthesis. The intermediate level led to the best recovery of photochemical efficiency; the low light required longer and the high light inhibited the extent of the recovered efficiency. It was concluded that the photosynthetic recovery of N. flagelliforme is both light-dependent and influenced by photon flux density.
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    URL: http://dx.doi.org/10.1023/A:1008011531325
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    Nitrogen availability influences the biochemical composition and photosynthesis of tank-cultivated Ulva rigida (Chlorophyta) (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 383-389 
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    ISSN: 1573-5176
    Keywords: ammonium ; C:N ratio ; tank culture ; dietary fibre ; fatty acids ; nitrogen ; photosynthesis ; Ulva rigida
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Physiological and biochemical changes in relation to inorganic nitrogen availability were studied for tank-cultivated Ulva rigida grown under nitrogen- enriched and nitrogen-depleted seawater. U. rigida was initially cultivated in nitrogen-enriched seawater (daily concentrations of NH4+ and NO3- + NO2- ranged between 0.5–1.7 and 0.06–0.15 mg L-1, respectively), then transferred to nitrogen-depleted seawater where photosynthetic capacity decreased to zero after 23 d. At the time (14 d) when photosynthetic rates were lower than 2.0 μmol O2 g-1 FW min-1 and strong bleaching had occurred, some algae were returned to the initial nitrogen-enriched seawater to study recovery from N-limited growth. Data on biochemical composition (chlorophylls, ash, caloric content, fatty acids and dietary fibres) and colouration varied significantly depending on the nitrogen conditions. C:N ratios correlated significantly with biochemical parameters. Fatty acid (FA) synthesis continued during the N-starvation period; saturated and mono-unsaturated FA increased to a maximun of 72.2%, while poly-unsaturated fatty acids (PUFA) decreased to 27.7%. During the N-enriched recovery period, the reverse was found. C:N ratios above 10 correlated with carbohydrate synthesis as shown by the dietary fibre level. Under nitrogen enriched conditions, C:N ratios decreased along with a decrease in fibre level. Under controlled conditions, nitrogen represents a major influence on the development of intensive tank cultivation of Ulva rigida, not only by affecting parameters closely related to nitrogen metabolism but also some clearly influenced by carbon uptake.
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    URL: http://dx.doi.org/10.1023/A:1008008912991
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    Unique response of Cyanophyceae to copper (1990)
    Springer
    Journal of applied phycology 2 (1990), S. 293-296 
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    ISSN: 1573-5176
    Keywords: heavy metal ; photosynthesis ; periphyton ; tolerance ; Cyanophyceae ; genetic adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study was made of the tolerance to Cu of 11 strains of Cyanophyceae and 7 strains of eukaryotes. These had all been tested within 6 months after isolation for their photosynthetic activity when exposed to Cu (Takamuraet al., 1989) and had repeatedly been subcultured in the medium without Cu for 2 years. Photosynthetic measurements were made in two ways: precultured in medium without Cu or precultured (for one subculture) in medium containing Cu (645 μg 1−1). The results were compared with those obtained within 6 months of isolation. The tolerance of the eukaryotes did not change significantly in any case, but most strains of Cyanophyceae lost their tolerance to Cu within a few subcultures in medium without Cu; however tolerance recovered following one subculture in medium containing an intermediate level of Cu. This rapid adaptation cannot be explained by a constitutive mutation.
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    URL: http://dx.doi.org/10.1007/BF02180917
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    Effects of environmental parameters on net photosynthesis of a free-living brown seaweed,Cystoseira barbata formarepens: determination of optimal photosynthetic culture conditions (1990)
    Springer
    Journal of applied phycology 2 (1990), S. 281-287 
    Details
    ISSN: 1573-5176
    Keywords: Cystoseira barbata ; photosynthesis ; light ; temperature ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The net photosynthesis of the Mediterranean brown seaweedCystoseira barbata f.repens is measured according to irradiance, temperature and salinity. There is not only, a good utilization of low light intensities (light-shade adaptation), but also a specific ability to use a broad range of irradiance, which corresponds in the photosynthesis-irradiance curves to a high initial slope and an extended light saturation level from 300 to 1500 μmol photon m−2 s−1; only very high irradiances induce photoinhibition. Maximum net photosynthesis occurred at temperatures ranging from 20 °C to 30 °C. The alga tolerates not only a low level of salinity, but also a slight increase in salinity; however, at more than 47.5 g 1−1 NaCl, oxygen exchange is significantly reduced. Light, temperature and salinity requirements are discussed, taking into account ecological considerations. Yields and quality of alginic acid are presented according to the irradiance and yearly evolutionin situ in order to aid future cultivation of this species.
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    URL: http://dx.doi.org/10.1007/BF02179786
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    Light response curves for Gelidium sesquipedale from different depths, determined by two methods: O2 evolution and chlorophyll fluorescence (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 295-301 
    Details
    ISSN: 1573-5176
    Keywords: Gelidium sesquipedale ; photosynthesis ; fluorescence ; light response curves ; pigments ; depth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photosynthesis-light response curves of Gelidium sesquipedale from the west coast of Portugal (Cape Espichel) were determined at four different depths, 3, 10, 15 and 22 m. Data acquisition using chlorophyll a fluorescence methodology and oxygen electrode measurements were compared. Response curves were determined over an increasing range of irradiance values (I), from darkness to 900 μmol photon m-2 s-1 PAR. In general, light response curves obtained for G. sesquipedale showed a similar pattern whether determined by the chlorophyll fluorescence method or by oxygen evolution. The photosynthetic capacity of G. sesquipedale decreased with depth, as expected, revealing a ‘sun’ and ‘shade’ acclimation pattern, between shallow and deeper waters.
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    URL: http://dx.doi.org/10.1023/A:1008057008447
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    Effects of solar UV stress on chlorophyll fluorescence kinetics of intertidal macroalgae from southern Spain: a case study in Gelidium species (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 285-294 
    Details
    ISSN: 1573-5176
    Keywords: UV-radiation ; chlorophyll fluorescence ; photosynthesis ; stress tolerance ; electron transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Photoinhibition and recovery kinetics after short exposure to solar radiation following three different irradiance treatments of irradiances (PAR, PAR+UVA and PAR+UVA+UVB) was assessed in two intertidal species of the genus Gelidium, Gelidium sesquipedale and G. latifolium, collected from Tarifa (southern Spain) using in vivo chlorophyll fluorescence (PAM fluorometry). After 3 h UV radiation exposure, optimal quantum efficiency (Fv/Fm) in G. sesquipedale decreased between 25 and 35% relative to the control. Under PAR alone, values decreased to 60%. In G. latifolium, photoinhibition did not exceed 40%. Similar results were found for the effective quantum yield (ΔF/Fm′), however, no marked differences in relation to light treatments were seen. When plants were shaded for recovery from stress, only in G. latifolium a significant increase in photosynthesis was observed (between 80 and 100% of control). In contrast, photosynthesis of G. sesquipedale suffered a chronic photoinhibition or photodamage under the three light irradiances. Full solar radiation (PAR+UVA+UVB) affected also the electron transport rate in both species. Here, initial slopes of electron transport vs. irradiance curves decreased up to 60% of controls. Although the recovery kinetic under PAR+UVA+UVB conditions was delayed in G. latifolium, after 24 h recovery this species reached significantly higher than G. sesquipedale. PAR impaired electron trasport only in G. sesquipedale. Overall, both species are characterized by different capacity to tolerate enhanced solar radiation. G. latifolium is a sun adapted plant, well suited to intertidal light conditions, whereas G. sesquipedale, growing at shaded sites in the intertidal zone, is more vulnerable to enhanced UV radiation.
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    URL: http://dx.doi.org/10.1023/A:1008021230738
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    Photosynthetic and population growth response of the test alga Selenastrum capricornutum Printz to zinc, cadmium and suspended sediment elutriates (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 145-151 
    Details
    ISSN: 1573-5176
    Keywords: 14C ; photosynthesis ; population growth ; Selenastrum capricornutum ; suspended sediment elutriate ; zinc ; cadmium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Short-term 14C-fixation (4 h) Selenastrum capricornutum algal toxicity tests were conducted with Cd (n=8), Zn (n=9) and suspended sediment aqueous elutriates (n=28) and the results were compared to those obtained in a 48 h population growth test. In order to provide more realistic experimental conditions, toxicity tests were carried out in prefiltered nutrient-spiked Lake Geneva water. The population growth inhibition test was significantly more sensitive than the14 C-fixation test for Cd (median EC50-4h and EC50-48h values of 600 and 118 µg L-1, respectively) whereas no significant difference was measured for Zn toxicity (median EC50-4h and EC50-48h values of 97 and 96 µg L-1, respectively). With suspended sediment aqueous elutriates, the relative sensitivity of the two different end points is sample dependent, with ratios of the EC25 for the14 C-fixation: population growth test ranging from 〈0.26 to 〉53.3. Elutriate toxicity shows no apparent relationship between the acute and chronic test, indicating that population growth inhibition cannot be derived directly or predicted from14 C-fixation. Both tests with their specific advantages and limitations provide valuable complementary information to measure the impact of single toxicants or complex mixtures on aquatic plants.
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    Reduced photoinhibition of a phycocyanin-deficient mutant of Synechocystis PCC 6714 (1998)
    Springer
    Journal of applied phycology 10 (1998), S. 447-452 
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    ISSN: 1573-5176
    Keywords: dense algal suspension ; light-harvesting pigment ; photosynthesis ; Synechocystis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects on photoinhibition of light-harvesting pigments in microalgal cells were examined using the wild type and a phycocyanin- deficient mutant (PD-1) of Synechosystis PCC 6714. Mutant PD-1 showed higher resistance to high light than the wild type in terms of the decline of photosynthetic activity at any light intensity and with various cell densities. This suggests that the loss of productivity induced by high light intensity would be improved by reducing the content of light-harvesting pigments.
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    URL: http://dx.doi.org/10.1023/A:1008049901939
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    Strain selection in Gracilaria spp. 2. Selection for high and low temperature resistance in G. verrucosa sporelings (1990)
    Springer
    Journal of applied phycology 2 (1990), S. 163-171 
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    ISSN: 1573-5176
    Keywords: Gracilaria ; strain selection ; growth ; photosynthesis ; rubisco ; agar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain selection procedure using Gracilaria verrucosa gametophytic sporelings was found to be an efficient tool for the improvement of Gracilaria strains. Two strains, C-2 and A-18, which were isolated and grown clonally, showed higher growth rates under high and low temperature conditions, respectively, than the local Gracilaria conferta. Growth rate, photosynthesis and chlorophyll, which were measured under different temperature and photon flux densities, demonstrated an overall advantage of the selected strains over the wild type strains of both G. verrucosa and G. conferta. Growth rates were also generally in positive correlation with the carboxylase activity of Rubisco. The G. verrucosa wild type also had a 40% higher agar content than G. conferta. The selected strains thus showed higher potential for outdoor cultivation than local wild type populations.
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    Photosynthate production in laboratory cultures (UV conditioned and unconditioned) of Cryptomonas erosa under simulated doses of UV radiation (1998)
    Springer
    Aquatic ecology 32 (1998), S. 297-312 
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    ISSN: 1573-5125
    Keywords: cryptomonads ; macromolecular ; Phototron ; photosynthesis ; UV radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used a device called a Phototron to measure the effects of UV radiation on the cosmopolitan algae, Cryptomonas erosa, grown in continuous cultures. In the Phototron, we investigated changes in photosynthetic parameters (Pmax – specific production rate at optimal light intensity; α – initial slope of the linear portion of the Photosynthesis-Irradiance curve; and θ – the convexity or rate of bending) and carbon allocation as a function of irradiance at three different environmentally-realistic doses of UV radiation in unconditioned (no prior UV exposure) and conditioned algae (15 d previous UV exposure). For unconditioned control algae, Pmax-Total was lower, although not significantly, than the two highest UV treatments. For conditioned control algae, Pmax-Total was higher, although not significantly, than all UV treatments. Our data suggest that short term (4 h) exposure to low levels of UV (8.09 W m−2 unweighted) does not affect Pmax-Total in C. erosa, but does change the proportion of carbon allocated to lipids and proteins. Also, comparisons of lipids, polysaccharides and proteins as a percent of total carbon uptake between unconditioned and conditioned algae indicate that exposure history to UV radiation can have a negative impact on carbon allocation to lipids and proteins, in a wetland alga species that is crucial to the efficient transfer of energy through freshwater food webs.
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    URL: http://dx.doi.org/10.1023/A:1009911811188
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    Characterization and transcriptional regulation of the Synechocystis PCC 6803 petH gene, encoding ferredoxin-NADP+ oxidoreductase: involvement of a novel type of divergent operator (1998)
    Springer
    Plant molecular biology 36 (1998), S. 353-363 
    Details
    ISSN: 1573-5028
    Keywords: ferredoxin-NADP+ oxidoreductase ; petH ; divergent operator ; antisense mRNA ; phosphoribulokinase ; prk Synechocystis PCC 6803 ; photosynthesis ; cyanobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The petH gene, encoding ferredoxin-NADP+ oxidoreductase (FNR), has been characterised in the unicellular cyanobacterium Synechocystis PCC 6803. Its product, FNR, was heterologously produced and functionally characterized. The start-site of the monocystronic petH transcript was mapped 523 bp upstream of the predicted PetH initiation codon, resulting in an unusually large 5′-untranslated region. The 5′ end of the petH transcript is situated within the open reading frame of phosphoribulokinase (encoded by prk), which is transcribed in opposite orientation with respect to petH. The transcription start site of the prk transcript was mapped 219 bp upstream of the initiation codon, resulting in a 223 bp antisense region between both transcripts. Under many conditions the expression of both genes (i.e. petH and prk) is co-regulated symmetrically at the transcriptional level, as was concluded from both northern hybridization experiments and from primer extension analyses; it became uncoupled, however, when specifically petH expression was stimulated, independent of prk expression, by stressing the Synechocystis cells with high salt concentrations. A model for a new type of bidirectional operator, regulating the expression of petH and prk, is proposed.
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    Interactive effects of elevated carbon dioxide and growth temperature on photosynthesis in cotton leaves (1998)
    Springer
    Plant growth regulation 26 (1998), S. 33-40 
    Details
    ISSN: 1573-5087
    Keywords: carbohydrates ; elevated CO2 ; Gossypium hirsutum L. ; interaction ; photosynthesis ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cotton (Gossypium hirsutum L., cv DPL 5415) plants were grown in naturally lit environment chambers at day/night temperature regimes of 26/18 (T-26/18), 31/23 (T-31/23) and 36/28 °C (T-36/28) and CO2 concentrations of 350 (C-350), 450 (C-450) and 700 μL L-1 (C-700). Net photosynthesis rates, stomatal conductance, transpiration, RuBP carboxylase activity and the foliar contents of starch and sucrose were measured during different growth stages. Net CO2 assimilation rates increased with increasing CO2 and temperature regimes. The enhancement of photosynthesis was from 24 μmol CO2 m-2 s-1 (with C-350 and T-26/18) to 41 μmol m-2 s-1 (with C-700 and T-36/28). Stomatal conductance decreased with increasing CO2 while it increased up to T-31/23 and then declined. The interactive effects of CO2 and temperature resulted in a 30% decrease in transpiration. Although the leaves grown in elevated CO2 had high starch and sucrose concentrations, their content decreased with increasing temperature. Increasing temperature from T-26/18 to 36/28 increased RuBP carboxylase activity in the order of 121, 172 and 190 μmol mg-1 chl h-1 at C-350, C-450 and C-700 respectively. Our data suggest that leaf photosynthesis in cotton benefited more from CO_2 enrichment at warm temperatures than at low growth temperature regimes.
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    The influence of Verticillium dahliae and drought on potato crop growth. 1. effects on gas exchange and stomatal behaviour of individual leaves and crop canopies (1990)
    Springer
    European journal of plant pathology 96 (1990), S. 273-289 
    Details
    ISSN: 1573-8469
    Keywords: Canopy enclosure ; stomatal conductance ; light response curve ; light use efficiency ; photosynthesis ; Solanum tuberosum L. ; transpiration ; water use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Aardappelplanten (Solanum tuberosum L.) cv.Saturna werden onderworpen aan stress als gevolg vanVerticillium dahliae en droogte. In vroege stadia van de groei werden stomataire geleiding, transpiratie en netto fotosynthese bij lichtverzadiging (PAR〉300 W m−2) gemeten aan individuele bladeren en met een mobiel instrumentarium met behulp van gewaskappen. Er werden geen significante verschillen gevonden in de waarden van de stomataire geleiding en de gasuitwisslingskarakteristieken als gevolg vanV. dahliae-besmetting tot een maand na opkomst. Daarna leidde infectie metV. dahliae tot een afname van de stomataire geleiding, transpiratie en netto fotosynthese, speciaal bij oudere bladeren en bij planten die meer aan zonlicht waren blootgesteld. Soms vertoondeV. dahliae interactie met droogte en bleken beide effecten minder dan optelbaar. De hoge waarden van de variatiecoëfficiënten maakten een groot aantal metingen per behandeling noodzakelijk; dit was vooral het geval bij metV. dahliae geïnfecteerde planten hetgeen aantoont datV. dahliae vooral in het begin van de groei niet alle bladeren in gelijke mate aantast. Door de matigende invloed van de integratie van alle bladlagen en mogelijk doordat de bovenste bladeren werden gestimuleerd, werd de totale gewasfotosynthese in mindere mate beïnvloed doorV. dahliae dan de individuele bladfotosynthese. De bovenste niet geïnfecteerde bladeren bleken verantwoordelijk voor het grootste gedeelte van de gewas-fotosynthese. De resultaten tonen aan, dat volgend op een infectie metV. dahliae, de fotosynthese reeds in een vroeg stadium van de groei wordt verminderd als een gevolg van droogtestress in de bladeren.
    Notes: Abstract Potato (Solanum tuberosum L.) plants cv.Saturna were subjected to infection withVerticillium dahliae and drought stress. At the early stages of growth, stomatal conductance, transpiration and net photosynthesis were measured at light saturation (PAR〉300 m−2) on individual leaves and with mobile field equipment with the aid of field enclosures. No significant changes in stomatal conductance and gas exchange characteristics occurred as a result ofV. dahliae instomatal conductance, transpiration and and photosynthetic rates, especially on older leaves and on plants exposed to direct sunlight for a longer period of time. In combination with drought,V. dahliae only occasionally showed interaction; their effects being less than additive. High values of coefficients of variatoon necessitated a high number of measurements per treatment; the more so in the inoculated plants which shows thatV. dahliae seems to affect certain leaves while not affecting others early in growth. Crop photosynthesis was less reduced byV. dahliae than individual leaf photosynthesis due to the levelling effect of integration over the whole canopy and possibly through a stimulation of the top leaves. The upper non-affected leaves are responsible for the bulk of photosynthetic crop activity. The results indicate that following an infection withV. dahliae photosynthesis is reduced early in growth as a result of drought stress in the leaves.
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    Ant nests as primary habitats ofSilybum marianum (Compositae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 209-217 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Silybum marianum ; Formicidae ; Messor semirufus ; Myrmecochory ; preadaptations ; synanthropic plant ; grasslands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The common Mediterranean ruderal thistleSilybum marianum is associated with nutrient-rich sites. Its wind-dispersed achenes possess an oily food body, that is attractive to harvester-ants. Following removal of the oily body, the achenes are deposited in the refuse zone together with rich organic material and soil removed from the nest; while in the nest the achenes are partly protected from fires. The thistle grows successfully in the nutrient-enriched refuse zone and thus dominates patches in the grassland. Preadaptations to live in association with harvester-ants enableS. marianum to occur also on marking stations of male gazelle, on cattle dung deposits, and in synanthropic ruderal habitats.
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    Systematics ofJusticia sect.Pentaloba (Acanthaceae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 219-235 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Acanthaceae ; Justicia ; Siphonoglossa ; Cytology ; flavonoids ; systematics ; taxonomy ; generic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The transfer of the four taxa ofSiphonoglossa sect.Pentaloba toJusticia is proposed. It is shown that the taxa of this section were placed inSiphonoglossa primarily because of a single-character phenetic relationship and that they correctly belong inJusticia. In addition to morphology, data from cytology and flavonoid chemistry are also presented that support this intergeneric transfer. A key to the taxa and a detailed taxonomic treatment of the section are provided.
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    A redescription ofPotamogeton distinctus including remarks on the taxonomy of thePotamogeton nodosus group (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 245-259 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Potamogetonaceae ; Potamogeton distinctus ; P. indicus ; P. nodosus ; P. tepperi ; Taxonomy ; distribution ; description
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Potamogeton distinctus has an E. Asian distribution and is closely related toP. nodosus. P. tepperi seems to be a synonym ofP. tricarinatus, even though the name has frequently been applied to other species likeP. distinctus. P. indicus is a synonym ofP. nodosus notwithstanding the fact that not all Indian broad-leaved pondweeds belong to that taxon.
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    Surface features of the leaves ofBalanophoraceae — A family without stomata? (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 29-35 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Balanophoraceae ; Balanophora ; Hachettea ; Langsdorffia ; Lophophytum ; Scybalium ; Thonningia ; Stomata ; epidermal surface
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Both adaxial and abaxial leaf surfaces were searched for stomata inBalanophora elongata, B. fungosa, Hachettea austro-caledonica, Langsdorffia hypogaea, Lophophytum mirabile subsp.mirabile, Scybalium jamaicense, andThonningia sanguinea (Balanophoraceae). Neither stomata nor guard cells were observed. The epidermal surfaces of these species are extremely diverse with respect to cell shape, cell size, and surface ornamentation, these features providing valuable systematic criteria.
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    Distribution of diploid sexual plants ofTaraxacum sect.Ruderalia in east-Central Europe, with special reference to Czechoslovakia (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 71-84 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Taraxacum sect.Ruderalia ; Sexual diploids ; mixed populations ; distribution pattern ; ecogeographical relations ; taxonomical problems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Herbarium specimens (c. 1 000) and population samples (76) have been screened for the occurrence of diploid representatives ofTaraxacum sect.Ruderalia (T. sect.Vulgaria, nom. illeg.). The area studied comprises German Democratic Republic, Poland, Czechoslovakia, Hungary and parts of Austria, Yugoslavia, and Rumania. Diploids are common from the Valley of the Moravia river on southwards. Isolated outpost localities are found in S. Poland and extend far to the north: neighbourhood of Berlin. Based on the pattern of distribution in Czechoslovakia, the Pannonian (thermophilous) phytogeographical character of the diploids is discussed. Special attention has been paid to morphological differences between di- and triploids in Czechoslovakia. The study provides a basis for further biosystematical and taxonomic studies in the relationships between diploids and polyploids as well as in the stability of agamospecies.
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    Pollination ofOphrys (Orchidaceae) in Cyprus (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 177-207 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Orchidaceae ; Ophrys ; bees ; Melecta ; Eucera ; Anthophora ; Andrena ; Pollination ; pseudocopulation ; Flora of Cyprus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the southern part of Cyprus the pollinator —Ophrys (Orchidaceae) relationships and its specifity have been investigated from the end of February until the middle of March 1986. 12Ophrys spp. were found. To date, only a single pollinator reference has been reported from this island. We found the following pollinators:Melecta tuberculata (Ophrys kotschyi),Eucera dimidiata (Ophrys flavomarginata),Eucera gaullei (Ophrys umbilicata),Eucera paulusi (Ophrys bornmuelleri),Anthophora erschowi (Ophrys elegans),Andrena torda (Ophrys sicula =O. lutea subsp.minor),Andrena cinereophila (Ophrys fusca, small-flowered),Andrena flavipes (Ophrys israelitica),Andrena morio (Ophrys iricolor andOphrys transhyrcana),Andrena bimaculata (Ophrys sphegodes aggr., probably formerly confused withO. transhyrcana). Most interestingly, it could be verified thatO. flavomarginata/O. umbilicata, O. bornmuelleri/O. levantina andO. transhyrcana/O. sphegodes aggr. (possiblyO. sintenisii) are different biospecies. This is a result of genetic isolation due to varying pollinators, and of differences in flower morphology.
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    Hair morphology and systematics ofPhysalis (Solanaceae) (1990)
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    Plant systematics and evolution 170 (1990), S. 193-204 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Solanaceae ; Physalis ; Hair morphology ; systematics ; hair development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hair morphology was exammed on developing seedlings and mature plants of ten species from five of seven series inPhysalis. The taxonomic importance of hair development and the distribution of hair types then was evaluated with respect to other comparative data. Two classes of hair types were observed, as found in earlier investigations ofSolanum andRhododendron. The first hair class comprises a sequence from unbranched hairs to dendroid-stelliform hairs. Hairs of this class show considerable variability among taxa in size, presence or absence of glandular tips, and presence and degree of branching, and so were useful in making taxonomic comparisons. The second hair class consists of short hairs, each tipped with a multicellular gland. This second hair class was not useful taxonomically because of its lack of variability in morphology and its often rare occurrence on a plant. The taxa studied exhibit distinctive trichome features which serve to distinguish series and, in some cases together with other data, provided new insight into previously enigmatic species relationships.
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    The cytotaxonomy ofEmilia spp. (Asteraceae: Senecioneae) occurring in Brazil (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 229-236 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Emilia ; Chromosome numbers ; C-banding ; cytogeography ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of several populations in a large part of the distribution area of the genusEmilia in Brazil has revealed only two species: the diploidE. sonchifolia and the tetraploidE. fosbergii. The more widely reportedE. coccinea was not found. They show a karyotype constancy in morphology and chromosome number (2n = 10 and 2n = 20, respectively), C-banding pattern and number of secondary constrictions. Some indications were found thatE. fosbergii may be an allopolyploid and that its ancestors had different genome sizes.
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    Reproductive biology ofPolyalthia littoralis (Annonaceae) (1990)
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    Plant systematics and evolution 170 (1990), S. 237-245 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Annonaceae ; Polyalthia littoralis ; Extended spiral thickenings ; flowering phenology ; reproductive biology ; self-pollination ; Malesian tropics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Flowering phenology of some annonaceous trees and reproductive biology ofPolyalthia littoralis (Annonaceae) were studied. The trees showed various types of flowering phenology within the family. Among them,P. littoralis had hermaphroditic and protogynous flowers, and exhibited continuous flowering throughout the year. Bagged flowers set fruits and seeds comparable to the control. The observations of meiotic stages and the results of castrated tests indicated no possibility of apomictic reproduction. The abscised anthers attached to torus by the extended spiral thickenings, and accomplished self-pollination. Outbreeding possibly occurs, but the following self-pollination guarantees seed set.
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    Pattern analysis in pseudanthia (1990)
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    Plant systematics and evolution 171 (1990), S. 57-88 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Inflorescence ; pseudanthium ; pseudocorolla ; flower symmetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pseudanthia occur in more than 40 angiosperm families. With regard to the underlying inflorescence structure they can be classified into the following groups: (a) floral and (b) hyperfloral pseudanthia, each with (c) or without (d) pseudocorollas. Pseudanthia have developed along independent evolutionary lines and are not bound to a particular inflorescence structure. They are the result of (a) the specific morphological predisposition of the taxon concerned, (b) aggregation and diminution of the flowers, giving rise to the formation of an attraction unit (for animal pollination), (c) variation, and (d) selection. Ontogenetical abbreviation is regarded to play an essential role in the origin and elaboration of pseudanthia.
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    Systematics and evolution within theCompositae, seen with the eyes of a chemist (1990)
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    Plant systematics and evolution 171 (1990), S. 1-14 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Chemistry ; systematics ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The broad knowledge of the chemistry of theCompositae allows the discussion of its relevance for the systematics and evolution within the family. Furthermore a separation into subfamilies can be supported by the observed differences in the distribution of the main constituents in the tribes.
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    Anthecology ofSchrankia nuttallii (Mimosaceae) on the tallgrass prairie (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 247-255 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Mimosaceae ; Schrankia nuttallii ; Halictidae ; Bee-pollination ; anthecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Schrankia nuttalii flowers through late spring on the tallgrass prairie. Although each stem produces an average of 26 capitate inflorescences only 12% of those inflorescences will open each day to disperse and receive polyads. Each inflorescence may live up to 48 hours but anthers abscise by late afternoon on the first day and the filaments change color and lose their scent. The 78–93 florets comprising each inflorescence open synchronously before dawn or during early morning hours. First day inflorescences ofS. nuttallii are herkogamous and fragrant. They are nectarless. Bombyliid flies and male bees are infrequent floral foragers so the major pollinators include female bees representing five families;Anthophoridae, Apidae, Colletidae, Halictidae, andMegachilidae. All foraging insects ignore second day inflorescences although stigmas are still receptive. Although 97% of all bees collected onS. nuttallii carrySchrankia polyads in their scopae or corbiculae 59% also carry the pollen/pollinaria of one or more coblooming angiosperms. At least 98% of all bees carrying mixed pollen loads incorporate the pollen/pollinaria of one or more nectariferous taxa (e.g.Asclepias spp.,Asteraceae, Convolvulaceae, Delphinium spec., etc.). Species of halictid bees are more likely to carry pure loads ofS. nuttallii polyads (70%) than bees of the four remaining families. Due to the nectarless florets and high degree of polylectic foraging bee-pollination inS. nuttallii converges more closely with the pollination systems of some AustralianAcacia spp. than with most other xeric/tropical genera of mimosoids studied in the western hemisphere.
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    The morphology and relationships ofMedusagyne (Medusagynaceae) (1990)
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    Plant systematics and evolution 171 (1990), S. 27-55 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Theales ; Medusagynaceae ; Medusagyne ; Systematics ; anatomy ; pollen ; morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The comparative vegetative and reproductive morphology and anatomy of the endangered, monotypic, dicotyledonous genusMedusagyne was studied, and detailed descriptions of leaf, axis, nodal, wood, floral, pollen, fruit, and seed structure are presented. Overall, the genus has many specialized features, including the possession of extreme, habitat-related specializations. Flowers are either bisexual or staminate, and are interpreted as retaining some primitive aspects, such as many free parts spirally arranged on an elongate floral axis. One of the most salient structural features of the plant is the massive development of ensheathing fibrous elements around the vascular system of both vegetative and reproductive tissues. Diffuse foliar sclereids are absent. Particular attention is paid to the unusual multicarpellate, synovarial gynoecium and comparisons are made with theCaryocaraceae. The totality of morphological and anatomical evidence confirms the view thatMedusagyne is a very distinct and evolutionarily isolated genus, best treated as forming the monotypic familyMedusagynaceae. Observations are presented, including the occurrence of stamen fascicle traces, that link the family to the dillenialean and thealean assemblage. Like other isolated thealean taxa,Medusagyne shows affinities to several different families, without having especially close relationships with any particular extant taxon.
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    Life cycle and adaptive strategies of three non-annual, ruderalCardueae: Onopordum nervosum, Carthamus arborescens, andCirsium scabrum (1990)
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    Plant systematics and evolution 171 (1990), S. 117-128 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Asteraceae ; Cardueae ; Onopordum nervosum ; Carthamus arborescens ; Cirsium scabrum ; Life cycle ; adaptive strategies ; autoecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The life cycle and autoecology of three Mediterranean thorny species,Onopordum nervosum (Iberian endemic),Cirsium scabrum (Western Mediterranean) andCarthamus arborescens (Iberian-North African), of potential use in agriculture as bioenergetic, forage or oil producing plants were studied. These threeCardueae spp. are good examples of ways in which Mediterranean plants adapt their typical annual cycle to avoid summer droughts.O. nervosum is a perennial, monocarpic, heliophilous species which flowers in early summer and grows even under the extreme xeric conditions of the Mediterranean summers. It is a basiphilous plant usually found in sandy-loamy and sandy-clayey-loamy soils with a marked geographical variability in its seed germination.C. scabrum is a perennial, monocarpic species which has a long life cycle, flowering in summer and growing only during the wet months. It is not well-adapted to droughts and is usually restricted to acid soils.C. arborescens is a perennial, polycarpic species which flowers in spring and dries up in summer. It is usually found in basic loamy-sandy or sandy-loamy soils poor in organic matter.
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    Phenotypic plasticity and character differentiation in a subdivided population ofIris pumila (Iridaceae) (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 1-9 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Iridaceae ; Iris pumila ; Phenotypic plasticity ; morphological variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variation patterns in phenotypic plasticity and broad sense heritability of 26 characters were examined within and among closely adjacent habitats of the bearded iris,Iris pumila. It was found thatI. pumila has considerable differentiation for phenotypic plasticity and genetic variation over short distances. An analysis of relationships between character differentiation and phenotypic plasticity suggests that they could have evolved independently. Possible mechanisms for maintaining local differentiation of the observed plastic and genetic variation are also discussed.
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    A redescription ofPotamogeton wrightii (Potamogetonaceae) (1990)
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    Plant systematics and evolution 170 (1990), S. 53-70 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Potamogetonaceae ; Potamogeton malaianus ; P. sumatranus ; P. wrightii ; Description ; distribution ; taxonomic evaluation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The type specimens ofP. malaianus Miquel collected byTeijsman are not identical withP. wrightii Morong, a species calledP. “malaianus” in Japan and elsewhere; they belong toP. nodosus. As a possible candidate for the correct name,P. sumatranus Miquel was studied: its exact identity, however, cannot be proved beyond doubt, even though the name refers at least partly toP. wrightii. That is whyPotamogeton wrightii Morong is proposed as the valid name of the species. A thorough description is provided including data on anatomy, morphology, life history, variability and ecology. A complete list of specimens, of synonyms and of misidentifications is given as well. The species shows a fuzzy structure with several special forms, intergrading hybrids; it is closely related with other taxa likeP. sumatranus. Still a great number of specimens cannot be assigned with certainty to any taxon.
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    Ribosomal DNA variation within and among individuals ofLisianthius (Gentianaceae) populations (1990)
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    Plant systematics and evolution 170 (1990), S. 97-106 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Gentianaceae ; Lisianthius ; Population variation ; ribosomal DNA ; isozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction endonuclease fragment analysis of nuclear ribosomal DNA (rDNA) was completed on 25 individuals each from seven populations of theLisianthius skinneri (Gentianaceae) species complex in Panama. Seven restriction enzymes were used to determine the amount and type of rDNA variation within and among individuals of the populations. No restriction site variation was seen within populations or individuals although site differences were seen among populations. Spacer length variation within and among individuals of populations was mapped to the internal transcribed spacer (ITS) region between the 18S and 5.8S rRNA genes, a region inLisianthius rDNA that previously was shown to exhibit length differences among populations. This is the first reported case of such variation within and among individuals of populations for the ITS region. Presence or absence of ITS spacer length variation is not correlated with levels of isozymic heterozygosity within populations. No detectable length variation within individuals or populations was seen in the larger intergenic spacer (IGS). Although populations varied with respect to IGS length, all individuals of a given population had a single and equivalent IGS length.
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    Chloroplast DNA diversity in the genusRubus (Rosaceae) revealed by Southern hybridization (1990)
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    Plant systematics and evolution 172 (1990), S. 65-75 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Rosaceae ; Rubus ; Chloroplast DNA ; restriction fragment length polymorphism ; cladistic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variability in chloroplast DNA type of 20Rubus genotypes was examined by Southern hybridization. DNA extracted from theRubus accessions was digested with two restriction enzymes (EcoRI and EcoRV) and heterologous chloroplast DNA sequences from barley and pea were used as probes to detectRubus chloroplast DNA sequences on Southern blots ofRubus total DNA. Restriction fragment length polymorphism was detected and a total of 92 restriction fragments were generated by the probe/enzyme combinations examined. Cladistic principles based on the parsimony assumption were used to assemble a phylogenetic tree based on chloroplast restriction fragment length data. The phylogenetic tree grouped the taxonomically defined species and is in general agreement with information based on morphological criteria. However, the Japanese red raspberryR. illecebrosus was shown to have diverged considerably in terms of evolutionary time from other species in subg.Idaeobatus. Furthermore, the molecular approach provides a quantitative estimate of the relationship between species that is difficult to obtain from morphological data. In order to complement the chloroplast DNA information a ribosomal DNA probe was also included in the analysis and provided further information on the phylogenetic relationships withinRubus.
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    Intraspecific chromosomal polymorphism ofTriticum araraticum (Poaceae) detected by C-banding technique (1990)
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    Plant systematics and evolution 169 (1990), S. 13-24 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticum araraticum ; Karyotype ; C-banding ; intraspecific divergence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DifferentTriticum araraticum lines were studied by C-banding method. The intraspecific divergence ofT. araraticum was shown to be caused mainly by large chromosomal rearrangements. Two main chromosomal types were distinguished among the studied lines: (1) a karyotype similar to that ofT. timopheevii and (2) different one. The first type includes some lines ofT. araraticum subspp.kurdistanicum andararaticum; the second comprises most lines ofT. araraticum subsp.araraticum. The lines of the first type can give fertile F1 hybrids withT. timopheevii.
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    A natural hybrid ofMonophyllaea (Gesneriaceae) in the tropical rain forests of West Sumatra (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 55-63 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Gesneriaceae ; Monophyllaea ; M. hirtella ; M. horsfieldii ; Chromosome number ; hybridization ; fertility ; tropical rain forest ; habitat segregation ; Flora of Indonesia ; Sumatra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A natural hybrid (2n = 21) between the parapatric rain forest speciesMonophyllaea hirtella (2n = 20) andM. horsfieldii (2n = 22) (Gesneriaceae) has been observed at Sg. Lubuk Paraku, Padang, W. Sumatra. The hybrids showed intermediary characters between the parental species in the inflorescence structure, flower size and colour, indumentum, chromosome numbers, and habitat. The 29% pollen fertility of a single hybrid specimen suggests that the genetical isolation between the parental species may break down and reproduction over subsequent generations may occur.
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    URL: http://dx.doi.org/10.1007/BF00935984
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    Cytogenetic investigations onOenothera nutans (Onagraceae) (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 69-80 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Onagraceae ; Oenothera sect.Oenothera subsect.Oenothera ; O. nutans ; Chromosomal analysis ; complex analysis ; structural heterozygosity ; complex heterozygosity ; taxonomy ; numerical taxonomy ; factor analysis ; reciprocal translocations ; Sifactors ; lethal factors ; sublethal factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Oenothera nutans, common to the Appalachian Mts between 650 and 1 700 m altitude, was investigated cytogenetically and taxonomically. The species is permanently structurally heterozygous. It consists of two genomes of the B-type which are more or less indistinguishable phenotypically. Nearly all of the strains investigated possess a self-incompatibility factor in one of the two complexes. Both complexes show a close relationship to the predominantly homozygousO. grandiflora, a native of the southern lowlands.O. nutans andO. grandiflora possess the same plastid type, plastome III. Probably,O. nutans evolved by an accumulation of reciprocal translocations within an originally structurally homozygous population, which must be regarded ancestral to the present forms ofO. grandiflora.
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    Pollen amino acids—an additional diet for a nectar feeding butterfly? (1990)
    Springer
    Plant systematics and evolution 169 (1990), S. 111-121 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Aesculus californica ; Amsinckia lunaris ; Brodiaea pulchella ; Carduus pycnocephalus ; butterflies ; Battus philenor ; Heliconius ; Pollen amino acids ; diffusion ; nectar ; butterfly-feeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The increase of the amino acid concentration over different time intervals in artificial nectar (i.e., a sucrose solution) due to pollen contamination was investigated in four Californian plant species (Aesculus californica, Amsinckia lunaris, Brodiaea pulchella, Carduus pycnocephalus), which are important nectar resources for a Californian colony of the butterflyBattus philenor as well as for other insects. The increase of the amino acid concentration in the medium is different in all four species and seems to be determined by a variety of factors including permeability of the pollen grain wall and presence or absence of pores. The results suggest a passive diffusion process of the free pollen amino acids into the medium rather than an active release. Implications from the experiments forBattus philenor and for other nectar feeding pollinators are discussed. A possible complementary effect of free pollen and nectar amino acids is proposed for plant species in which pollen is likely to be knocked into nectar by their flower visitors. A possible evolutionary pathway from nectar feeding butterflies such asBattus philenor to the complex derived pollen feeding habit in theHeliconius butterflies is proposed.
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    The floral biology ofThelymitra epipactoides (Orchidaceae), and the implications of pollination by deceit on the survival of this rare orchid (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 11-27 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Orchidaceae ; Thelymitra epipactoides ; bees ; Nomia ; Lasioglossum ; Pollination ; deceit ; floral mimicry ; Flora of Australia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thelymitra epipactoides has a highly variable visual display achieved through polychromatic flowers and variable inflorescence size, bearing between 7 and 31 flowers, which attract foraging polylectic bees. Only bees of the genusNomia were observed carrying pollinia and successfully pollinating the orchid. The genusNomia contains polylectic, pollen gathering species that store pollen in both the crop and scopa on the hind legs. The absence of a reward for the bees indicates the orchid is relying on deception to attract visitors. The relationship of deception to mimicry is discussed. Once on the flower, tactile, visual and possibly olfactory stimuli direct bees to the false anther formed by the voluminous column wings, where morphological adaptations of the flower ensure that the pollinarium is deposited on the gaster of the bee to effect pollination. — The lack of seed set observed on the Victorian coast appears to be due to the absence of pollinators from the heath and grassland communities in which the orchid grows. This may well be a consequence of the reduced number of plants flowering in the community (a result of the elimination of fire at these sites), thus not maintaining a floral community attractive to potential pollinators.
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    Pollination ofDianthus gratianopolitanus (Caryophyllaceae) (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 125-132 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Caryophyllaceae ; Dianthus gratianopolitanus ; Pollination ; psychophily ; phalaenophily ; nectar composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pollination ofDianthus gratianopolitanus was studied in a population of the Swiss Jura mountains. Pollinators of this plant species are reported here for the first time. The flowers were not only visited by butterflies as postulated in the literature, but also by diurnal hawkmoths (Macroglossum stellatarum) and by diurnal and nocturnal noctuid moths. — Nectar is sucrose-dominant, the sugar concentration is moderate but the amino acid concentration is high. Nectar characteristics correspond well with the syndrome ofLepidoptera-pollinated flowers. — Field observations and flower characters (colour, range of the calyx length) suggest thatDianthus gratianopolitanus is an intermediate species in the transition of butterfly to moth pollination. — Lack of reproductive success inDianthus gratianopolitanus can not be attributed to lack of suitable pollinators.
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    “Ixoroid” secondary pollen presentation and pollination by small moths in the Malagasy treeletIxora platythyrsa (Rubiaceae) (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 161-175 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Rubiaceae ; Ixora ; I. platythyrsa ; Moth-pollination ; secondary pollen presentation ; Flora of Madagascar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pollination biology ofIxora platythyrsa (Rubiaceae) was studied in NW. Madagascar. The plant displayed cream-yellow, nocturnally fragrant, nectariferous, tubular and strongly protandrous flowers. These had an “ixoroid” secondary pollen presentation mechanism: prior to anthesis, anthers exhausted their pollen onto unripe stylar heads. From this position pollen of male-stage flowers later adhered to primarily the probosces of small visiting nocturnal noctuid and geometrid moths. — Pollen was subsequently raked off moths' probosces by receptive, copiously papillose stigmas of female-stage flowers. Principal pollination adaptation was probably to the noctuid moth subfam.Sarrothripinae.
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    The systematic significance of surface features of theBalanophora tuber (Balanophoraceae) (1990)
    Springer
    Plant systematics and evolution 171 (1990), S. 129-134 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Balanophoraceae ; Balanophora ; Tuber surface morphology ; systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The surface ofBalanophora tubers consists of a nonepidermal layer made up of two distinctive types of cells, armature cells and stellate wart cells. Both cell types are provided with a heavy wall, and are dead at maturity. Stellate warts in the three species investigated,B. elongata, B. fungosa, andB. hansenii, séem to be uniform in appearance, but armature cells are extremely distinctive for each species. They are present in large, agglomerate masses in the first, singly or in very small clusters in the second, and as completely free, individual, acicular cells in the third species. Such differences are believed to be significant systematically, and the separation ofB. hansenii is thus probably justified. Notwithstanding superficial similarities, stellate warts do not seem to be comparable to the lenticels of other plants.
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    Ribosomal DNA spacer-length variation inSecale spp. (Poaceae) (1990)
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    Plant systematics and evolution 171 (1990), S. 205-220 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Secale ; Ribosomal DNA ; spacer subrepeats ; restriction fragment length polymorphism (RFLP) ; phenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variation in ribosomal DNA spacer length was analysed in 23 populations of 12Secale spp. by restriction enzyme analysis. Digestion of rDNA with Taq I endonuclease enzyme yields spacer fragments that include the subrepeat array and the non-repetitive region downstream of the array. Extensive spacer length variation existed in most species with Taq I fragment lengths ranging from 0.9–3.1 kb. These length variants have been attributed to the differences in number of 134 bp spacer subrepeats within rDNA arrays.S. silvestre was the only species to exhibit a unique spacer length variant of 0.9 kb and this was shown to result from the presence of an extra Taq I site in the spacer. rDNA spacer length frequencies were determined for the species. These frequencies were used to derive phenetic relationships between the species by numerical taxonomic methods. In plots constructed fromGower's distance matrices,S. silvestre appeared well separated from the major cluster consisting of the other species. On the basis of morphological and cytogenetic criteria,S. silvestre is considered the most ancient species. The rDNA data is consistent with this interpretation as it shows a clear differentiation ofS. silvestre from all the other species based on length and nucleotide sequence composition of the spacer region.
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    Identification and partial characterization of two species-specific repeat families in the great millet (Sorghum vulgare, Poaceae) genome (1990)
    Springer
    Plant systematics and evolution 171 (1990), S. 249-257 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Sorghum vulgare ; great millet ; DNA hybridization ; repetitive DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 1.4 kbp Xba I and the 1.3 kbp EcoRI repeat families in great millet were partially characterized with respect to their genomic distribution and their homology with the EcoRI and Xba I families of five other millet DNAs. The digestions of great millet DNA using increasing amounts of the two enzymes show that these two families are disperse in nature. The hybridization of these two families to the genomic digests of great millet indicates that they are arranged in a clustered and scrambled manner. Similarly, the hybridization with the EcoRI and Xba I digests of five other millet DNAs reveals the speciesspecific nature of these two repeat families. The latter also hybridize to the total repetitive fraction of great millet isolated at a highly stringent temperature of 75°C suggesting that the members of these two families are probably largely homogeneous.
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    Pollination ecology ofOxalis violacea (Oxalidaceae) following a controlled grass fire (1990)
    Springer
    Plant systematics and evolution 171 (1990), S. 147-155 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Oxalidaceae ; Oxalis violacea ; Andrenidae ; Bombyliidae ; Halictidae ; Megachilidae ; Pollination ecology ; heterostyly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vernal grass fires may encourage profuse flowering in clonal, colonies ofOxalis violacea. Long-styled colonies appear to be more floriferous than short-styled colonies and set a greater number of capsules. Individual flowers of both morphs live one or two days, change position on their respective pedicels and advertise nectar concealed at the base of the floral throat. AlthoughDiptera, Hymenoptera, andLepidoptera forage for nectar, bees (Andrenidae,Anthophoridae, Halictidae, andMegachilidae) probably make the only effective pollen transfers between the two morphs. Both male and female bees may transport pollen of both morphs and short-tongued bees (e.g.,Augochlorella spp.,Dialictus spp.) may be more common but as effective as pollinators as long-tongued bees (e.g.,Calliopsis andreniformis andHoplitis spp.). The conversion rate of flowers into capsules is only 13–17%. The spreading style in the short-styled morph is interpreted as an adaptation restricting insect-mediated, self-pollination but encouraging bee-stigma contact during nectar foraging.
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    Sympodial and monopodial branching inAcer (Aceraceae): Evolutionary trend and ecological implications (1990)
    Springer
    Plant systematics and evolution 171 (1990), S. 187-197 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Aceraceae ; Acer ; Sympodial and monopodial branching ; evolution ; adaptive strategy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The evolutionary trend and its ecological implications in sympodial and monopodial branching patterns has been investigated in 20 JapaneseAcer spp. through comparison of shoot tip abortion and terminal bud formation. The genus is divided into two species groups according to its branching pattern, one (6 species) predominantly exhibiting sympodial branching with frequent monopodial branching in short shoots (sympodial species), and the other (14 species) exhibiting only monopodial branching (monopodial species). The early ontogeny of leaf and bud scales is described. Despite the difference in branching patterns, the bud scales of terminal buds are essentially the same in having a leaf base developed to function as a protecting organ. In all the sympodial species, during the abortion of a sympodium shoot tip, one or two pairs of primordia were found to occur on the apex, and later wither. These primordia resemble bud scales of terminal buds in their ontogeny and morphology, and appear to be rudimentary. It is suggested that a rudimentary terminal bud develops together with the establishment of sympodial branching, and that sympodial branching has originated from monopodial branching. Based on this proposed evolutionary trend, it is suggested thatAcer has moved from less shady habitats into shady habitats with monopodial branching (advantageous for vertical growth) changing into sympodial branching (advantageous for lateral spread).
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    Crossing experiments of lettuce cultivars and species (Lactuca sect.Lactuca, Compositae) (1990)
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    Plant systematics and evolution 171 (1990), S. 233-248 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Lactuca sativa ; L. serriola ; L. saligna ; L. virosa ; Hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The degree of relationships withinLactuca sativa and three wild relativesL. serriola, L. saligna, andL. virosa was studied by observing the performance, vigour and fertility of the F 1 hybrids obtained from crosses made in and between the four species. The crosses ofL. saligna ×L. virosa and the reciprocal crosses produced no hybrids.L. saligna andL. virosa are the least related of the four species.L. sativa ×L. serriola and the reciprocal crosses were successful and produced fertile hybrids These two species are genetically very closely related.L. saligna is known to produce, as a female parent, hybrids withL. sativa andL. serriola. Now the reciprocal cross was successful for the first time, so the unability to obtain hybrids in the past was based on the choice of accessions and not caused by unilateral incompatibility.L. virosa ×L. sativa and the reciprocal combination produced hybrids. The combinationL. serriola ×L. virosa produced hybrids with very limited fertility. In contrast to earlier reports (sterile hybrids) one combination of the reciprocal cross too produced hybrids with very limited fertility.—Some of theL. saligna ×L. sativa (and reciprocal) hybrids were found to look strikingly likeL. serriola. This adds evidence for the descent ofL. serriola andL. sativa:L. saligna also made part of the ancestral complex of the cultivated lettuce.
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    Genetic divergence and introgressive hybridization betweenAlnus sinuata andA. crispa (Betulaceae) (1990)
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    Plant systematics and evolution 170 (1990), S. 107-124 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Betulaceae ; Alnus ; Isozymes ; population genetics ; hybridization ; genetic distance ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The actinorhizal genusAlnus contains numerous taxa that have been morphologically classified into different subgenera, species and subspecies. The genetic divergence has been evaluated within subg.Alnobetula between the parapatric taxaAlnus sinuata andA. crispa, using diversity of allozyme markers at 15 structural loci among 20 populations. Evidence for introgressive hybridization at the overlap of their ranges was noted in three populations. However, the width of the hybrid zone appeared tenuous. The average genetic distance derived from the comparisons of conspecific populations was much smaller than the interspecific distance (D = 0.047). This allelic divergence was also paralleled with larger amounts of allelic and genotypic diversity within and among populations ofA. sinuata, which are occupying a more heterogenous ecological niche. It is proposed that the repeated advances and retreats of the ice sheet during the Pleistocene may have promoted the divergence and allopatric evolution of these subspecies, and that secondary contact may have occurred repeatedly during the interglacial periods. The dynamic-equilibrium model would predict in such cases that narrow hybrid zones, formed at the contact of parapatric ranges, would impede gene exchange between parental taxa by selection against hybrids. The results obtained in this study seemed concordant with this hypothesis, as they were also in agreement with the existent taxonomical treatment of these taxa based on morphology.
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    Karyological studies inPelargonium sectt.Ciconium, Dibrachya, andJenkinsonia (Geraniaceae) (1990)
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    Plant systematics and evolution 170 (1990), S. 151-159 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Geraniaceae ; Pelargonium ; Chromosome numbers ; karyotypes ; karyotype evolution ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pelargonium sect.Ciconium and sect.Dibrachya have a basic chromosome number of x = 9, whereas sect.Jenkinsonia has x = 9, 11, and 17.
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    SEM observations on seeds ofStriga spp. andBuchnera americana (Scrophulariaceae) (1990)
    Springer
    Plant systematics and evolution 170 (1990), S. 257-263 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Scrophulariaceae ; Striga ; Buchnera ; Seed coat morphology ; systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seeds of the root parasitesStriga (several spp.) andBuchnera americana were examined by means of SEM. The surface patterns of the seeds in both genera resemble each other closely, especially those ofS. angustifolia andB. americana. SomeStriga spp. can be clearly distinguished by their surface characteristics, while this is quite difficult in others. The taxonomic value of the seed surface features ofStriga andBuchnera is discussed.
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    Delimitation ofPelargonium sect.Glaucophyllum (Geraniaceae) (1990)
    Springer
    Plant systematics and evolution 171 (1990), S. 15-26 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Geraniaceae ; Pelargonium sect.Glaucophyllum ; Morphology ; pollen ; chromosome numbers ; flavonoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pelargonium otaviense Knuth andP. spinosum Willd. are excluded from sect.Glaucophyllum, whileP. grandiflorum (Andr.)Willd.,P. patulum Jacq. andP. tabulare (Burm. f.)L'Hérit. of sect.Eumorpha are included. Sect.Glaucophyllum is characterized by green to glaucous vegetative organs and zygomorphic white to pink corolla with five narrow petals. All the species have an identical pollen and chromosome morphology, the same basic chromosome number (x = 11) and similar flavonoid patterns. A close relationship between sect.Glaucophyllum and sect.Pelargonium is indicated by the occurrence of natural hybrids and concordant characters. Isorhamnetin and luteolin have been detected in the genus for the first time.
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    Molecular divergence in the Galapagos Islands—Baja California species pair,Gossypium klotzschianum andG. davidsonii (Malvaceae) (1990)
    Springer
    Plant systematics and evolution 171 (1990), S. 99-115 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Malvaceae ; Gossypium ; Chloroplast DNA ; allozymes ; isozymes ; molecular evolution ; long-distance dispersal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Molecular divergence betweenGossypium klotzschianum andG. davidsonii was studied. The former is endemic to five of the larger islands of the Galapagos, whileG. davidsonii is restricted to the southern half of Baja California, approximately 2 500 km distant. A substantial body of genetic and taxonomic data suggests that these two species are related as progenitor and derivative. Interspecific hybrids are fully fertile, with no evidence of F2 breakdown and normal segregation of genetic markers. Allozyme analysis of 33 populations for 41 loci indicated that the allelic composition ofG. klotzschianum represents a subset ofG. davidsonii. Although genetic diversity is relatively restricted in both species, calculated measures demonstrate higher levels of genetic variability and greater population structuring inG. davidsonii than inG. klotzschianum. The interspecific genetic identity of 0.87 is typical for progenitor-derivative species pairs. Chloroplast DNAs were surveyed for variation with 25 restriction enzymes using hybridization probes that cover the entire chloroplast genome. No intraspecific and little interspecific variation was detected among 560 cpDNA restriction sites, representing sequence information for approximately 3200 nucleotides. Only 3 mutational differences distinguished the two species, resulting in a sequence divergence estimate of 0.09%. Divergence times were estimated from both the isozyme data and the cpDNA restriction site data. Although these estimates have several sources of error, both molecular data sets were congruent in suggesting that the two lineages diverged between 250000 and 700000 years ago. Accumulated evidence suggests that dispersal was from Baja California to the Galapagos Islands rather than the reverse, and most likely was mediated by trans-oceanic drift.G. klotzschianum may be the only species of the endemic Galapagos flora to have arisen from a northern Mexican progenitor.
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    The systematic position of theHolarrheninae (Apocynaceae) (1990)
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    Plant systematics and evolution 171 (1990), S. 157-185 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Apocynaceae ; Plumerioideae ; Apocynoideae ; Holarrheninae ; Holarrhena ; Carruthersia ; Spirolobium ; Floral structure ; pollen morphology ; indole alkaloids ; steroidal alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genusHolarrhena, described byRobert Brown in 1811, has had a problematic taxonomic history, in part due to a suite of characters that does not conform with accepted concepts within theApocynaceae. In a number of important taxonomic charactersHolarrhena is typical of subfam.Apocynoideae. But due to the relatively unspecialized structure of the anthers most recent authors have placedHolarrheng, together withCarruthersia andSpirolobium, as the subtribeHolarrheninae in subfam.Plumerioideae. For the present investigation the floral structure and pollen morphology ofHolarrhena, Carruthersia andSpirolobium were analyzed. From the chemical literature reports of the occurrence of steroidal alkaloids in thePlumerioideae were evaluated. Our results indicate that the three genera belong to subfam.Apocynoideae in the tribeNerieae, but that the “Holarrheninae” is an unnatural group, and that the three genera should be accommodated individually within the tribe.
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    Ultrastructural evidence of endoplasmic reticulum changes during the maturation of the olive pollen grain (Olea europaea L., Oleaceae) (1990)
    Springer
    Plant systematics and evolution 171 (1990), S. 221-231 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Oleaceae ; Olea europaea ; Endoplasmic reticulum ; stacks cisternae ; pollen grain ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Characteristic features of rough endoplasmic reticulum (rER) distribution and proliferation were noted during olive pollen (Olea europaea L.) development, suggesting the physiological significance of this organelle. Initially scarce in the young microspore, ER increases as cytoplasmic vacuoles form. At the vacuolated microspore stage the cytoplasm contains numberous polysomes and elongated rER cisternae arranged preferentially in stacks, with an average intracisternal width of 0.07 µm. Stacks persist in the bicellular pollen grain but consist of fewer, shorter, dilated cisternae (mean intracisternal width 0.1 µm) containing a considerable electron-dense matrix. Cisternae in the mature grain are fragmented, leaving behind an ER of swollen pockets. Pockets of ER containing a material of greater electron density are evenly deposited along the plasmalemma, in close relation with it. A dense material is seen in the tubules of the apertural region, which was lacking in earlier stages. Our results show that ER may be involved in protein transport to the intine.
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    Pollination biology of two species ofKielmeyera (Guttiferae) from Brazilian cerrado vegetation (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 35-49 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Guttiferae ; Kielmeyera coriacea ; K. speciosa ; “Buzz pollination” ; carpenter bees ; breeding system ; andromonoecy ; self-mimicry ; neotropical savanna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pollination biology and breeding systems ofKielmeyera coriacea andK. speciosa, two sympatric woody species common in the cerrado vegetation of C. Brazil, were studied. Both species have similar nectarless, polystemonous “Papaver-type” flowers which are visited by a similar spectrum of insects, though they bloom in different seasons and are thus phenologically isolated. Large carpenter bees seem to be the most important pollinators and these and other bees effect “buzz pollen” retrieval despite the fact that anthers are not poricidal. Both species ofKielmeyera possess strong xenogamous breeding systems. The presence of staminate flowers and andromonoecy inK. coriacea, as well as the longevity ofK. speciosa flowers are discussed as alternative strategies to improve pollination success and reproductive efficacy.
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    Chemotaxonomic study of the genusTabernaemontana (Apocynaceae) based on their indole alkaloid content (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 13-34 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Apocynaceae ; Tabernaemontana ; Indole alkaloids ; chemotaxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract According to their alkaloidal products species of the “new” genusTabernaemontana can be partly differentiated. This differentiation is in agreement with the “old” genera classification. From the chemotaxonomic point of view a subdivision of subfam.Plumerioideae of theApocynaceae is proposed.
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    A phenetic comparison of someFumaria spp. (Fumariaceae) (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 51-63 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Fumariaceae ; Papaveraceae ; Fumaria ; Phenetics ; numerical taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The taxonomy of the genusFumaria has not been considered in detail sincePugsley's work in 1919 ff., and few modern methods have been applied to it. In a phenetic study, 33 populations of 11Fumaria spp. were grown in uniform conditions, and seven morphological characters measured. After re-expression and transformation the data were analysed by cluster analysis and principal components analysis. Alternative analyses did not indicate contradictory taxonomic conclusions. Artificial crosses gave some evidence on interfertility, and suggestedF. occidentalis to be an allopolyploid ofF. bastardii ×F. capreolata. Pugsley's subsectional classification is supported within sect.Parviflora, but not within sect.Grandiflora. His two sections are seen to be meaningful, but not discrete.
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    A phylogenetic analysis of chloroplast DNA restriction site variation inPoaceae subfam.Pooideae (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 83-97 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Pooideae ; Phylogenetics ; evolution ; chloroplast DNA ; restriction site variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A phylogenetic analysis was conducted on chloroplast DNA restriction site variation in 34 genera of grasses (familyPoaceae), including 28 genera from subfam.Pooideae (representing tribesAveneae, Brachypodieae, Bromeae, Meliceae, Poeae, Stipeae, andTriticeae) and representatives of three other subfamilies,Arundinoideae, Oryzoideae, andPanicoideae. Analyses of all 34 genera always distinguishedPooideae as monophyletic, regardless of which nonpooid genus functioned as outgroup; six separate analyses of all 28 pooid genera, each including one of the six nonpooid genera as outgroup, resolved five identically-constituted clades withinPooideae (in four cases), or (in the other two cases) yielded results that were less well resolved, but not in conflict with those of the other four analyses. The four best-resolved analyses distinguishedMeliceae as the earliest diverging lineage withinPooideae, andStipeae as the next. Above the point of divergence ofStipeae is a dichotomy between supertribeTriticodae (including tribesBrachypodieae, Bromeae, andTriticeae), and a clade comprisingPoeae andAveneae. The analysis supports some tribal realignments, specifically the assignment ofBriza, Chascolytrum, Microbriza, andTorreyochloa toAveneae, andArctagrostis, Catabrosa, andSesleria toPoeae. The analysis also suggests that the pooid spikelet (i.e., glumes shorter than lemmas and florets two or more) is plesiomorphic inPooideae, and that spikelets with one floret, and those with glumes longer than the first lemma, each have evolved more than once withinPooideae. Results also indicate that small chromosomes and chromosome numbers based on x=c. 10−12 are plesiomorphic withinPooideae. Alternative states of these characters (chromosomes large, chromosome numbers based on x=7) are interpreted as synapomorphies or parallelisms of clades that includeTriticodae, Aveneae, andPoeae. Lanceolate lodicule shape may be a synapomorphy of the clade that includesStipeae, Triticodae, Aveneae, andPoeae, and loss of lodicule vascularization a synapomorphy of the entirePooideae.
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    A phylogenetic and allozyme approach to understanding rarity in three “green ash” eucalypts (Myrtaceae) (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 99-118 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Myrtaceae ; Eucalyptus ; Rarity ; allozymes ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phylogenetic relationships among the 12 species of the “green ash” group of eucalypts were examined using allozyme data, to investigate the causes of rarity in three localized endemics of the group. The relationships suggested by the allozyme data showed both similarities to and differences from those estimated from morphological data byLadiges and coworkers. The phylogenetic relationships suggest that rarity inEucalyptus burgessiana may be related to recent divergence, whileE. paliformis andE. rupicola are relatively old species, more likely to be relicts, and/or restricted to a rare habitat.
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    Self-incompatibility inCrocus vernus subsp.vernus (Iridaceae) (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 77-82 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Iridaceae ; Crocus vernus subsp.vernus ; Embryology ; mentor effect ; pollen tube ; self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Outcross, self- and mixed pollinations were performed inCrocus vernus subsp.vernus, a species with bicellular pollen, dry stigmas and hollow style. No differences were noted among the above pollinations concerning the germination of pollen and the growth of pollen tubes until the top of ovary. Within 45 min after pollinations 62% of pollen grains germinated. Pollen tubes penetrated the papilla cuticle extending along the papilla wall; on entry into stigmatic lobes they continued growth in the stylar secretion to ovarian locules. Here, however, self-pollen tubes failed to reach or to enter the ovule micropyle; while pollen tubes from either outcross- or mixed pollinations grew until fertilizing ovules. These observations gave evidence of a self-incompatibility system inCrocus, which appeared to be neutralized by mentor effect. The ovary as site of incompatibility response is discussed.
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    Giemsa C-banded karyotypes of diploid and tetraploidHordeum bulbosum (Poaceae) (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 141-150 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Hordeum bulbosum ; Cytotypes ; chromosome analysis ; Giemsa C-banding patterns ; autopolyploidy ; relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The similar-looking basic genomes ofHordeum bulbosum (2x and 4x) have five rather similar metacentric, one submetacentric, and one satellited choromosome. C-banding patterns are characterized by one or two centromeric, or juxtacentromeric, small to larger bands in most chromosomes, by bands at the nucleolar organizers, by small or very small telomeric bands, and by the nearly complete lack of intercalary bands. Banding pattern polymorphism is widespread. Banding patterns supported by chromosome morphology enable identification of homologues, and discrimination between non-homologues inH. bulbosum (2x). The C-banded karyotype ofH. bulbosum (4x) supports an autopolyploid origin, but it was possible to identify only homologues of submetacentrics and SAT-chromosomes.
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    Restriction fragment length polymorphisms in the genusActinidia (Actinidiaceae) (1990)
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    Plant systematics and evolution 172 (1990), S. 193-203 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Actinidiaceae ; Actinidia ; Chloroplast genome ; kiwifruit ; molecular evolution ; phylogenetic trees ; restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A series of chloroplast and nuclear probes were used to examine restriction fragment length polymorphisms (RFLPs) in kiwifruit (Actinidia deliciosa) and three of its closest relatives. The four species fell into two pairs, withA. chinensis andA. deliciosa closely related but some distance away from the other two species,A. latifolia andA. eriantha. The results are consistent with the hypothesis that the diploid species,A. chinensis, is a precursor ofA. deliciosa, which is hexaploid.
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    Ribosomal RNA genes inQuercus spp. (Fagaceae) (1990)
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    Plant systematics and evolution 172 (1990), S. 127-139 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Fagaceae ; Quercus ; Gene mapping ; ribosomal RNA genes ; rRNA/DNA hybridization ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The taxonomy of the genusQuercus is still unclear. In order to elucidate the taxonomy of Mediterranean oaks we have analyzed ribosomal RNA genes ofQuercus cerris, Q. coccifera, Q. trojana, Q. ilex, Q. suber, andQ. macrolepis by means of Southern blot hybridization. Oak nuclear DNA was extracted from root tips of 300 acorns and from catkins of single plants. EcoRI and BamHI restriction endonucleases were used. DNA electrophoresis and rRNA/DNA hybridization were performed usingVicia faba rRNA 18 S and 25 S as probes. The rRNA genes of all the species studied have an identical restriction mapping in the 18 S and 25 S regions, while differences in length are present in the intergenic regions.Q. cerris possesses at least four types of genes of 12.1, 11.5, 8.5, and 8.3 kb;Q. coccifera at least three types of 12.4, 10.4, and 10.1 kb;Q. trojana possesses the same rRNA genes asQ. cerris plus another gene type 12.0 kb long, with EcoRI and BamHI restriction sites in the intergenic spacer;Q. ilex at least three types of 12.4, 10.85, and 9.5 kb;Q. suber at least five types of 11.5, 11.0, 8.6, 8.5, and 8.3 kb;Q. macrolepis, finally, at least seven types of 11.5, 11.0, 10.2, 8.6, 8.5, 8.3, and 8.15 kb.Q. coccifera andQ. ilex rDNA appears quite different respect to other species examined, while high similarity seems to exist betweenQ. cerris, Q. trojana, Q. suber, andQ. macrolepis. These results are in agreement with the taxonomic model proposed bySchwarz for the genusQuercus.
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    Evolution of the tetraploidCapsella bursa-pastoris (Brassicaceae): Isoelectric focusing analysis of Rubisco (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 205-213 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Brassicaceae ; Capsella ; Ribulose-1,5-bisphosphate carboxylase/oxygenase ; isoelectric focusing ; evolution ; polyploidization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) and its subunits (large subunits = LSU, small subunits = SSU) were isolated from threeCapsella spp. by gel electrophoresis and polypeptide composition was analyzed by isoelectric focusing (IEF) in the presence of 8M urea. The described techniques are recommended for large scale systematic studies. Multiple IEF banding patterns of the SSU are probably the outcome of a heterogenous multigene family. The two diploid speciesC. rubella andC. grandiflora show an identical IEF pattern and could be differentiated from the putative allotetraploidC. bursa-pastoris only by the SSU banding pattern. Uniqueness of some SSU bands in the tetraploid and in the two diploid species, respectively, may indicate an ancient alloploid origin of tetraploidC. bursa-pastoris followed by events leading to divergences in the genomes of the allotetraploid and its presumed diploid progenitors after the hybridization event (SSU gene elimination, acquisition of new SSU genes).
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    Heterostyly in species ofNarcissus (Amaryllidaceae) andHugonia (Linaceae) and other disputed cases (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 215-227 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Amaryllidaceae ; Narcissus ; Linaceae ; Hugonia ; Bauhinia ; Cleome ; Aneilema ; Agelaea ; Heterostyly ; tristyly ; distyly
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hypothesis ofHenriques andFernandes that several Iberian species ofNarcissus (Amaryllidaceae) are tristylous is reconsidered. Contrary to the opinion ofBateman and most subsequent authors, we believe that the available evidence indicates that some populations ofN. triandrus andN. fernandesii, at least, are tristylous; other populations ofN. triandrus are distylous.Hugonia cf.penicillanthemum (Linaceae) from new Caledonia is distylous, but it remains possible that other species ofHugonia are tristylous. The disputed occurrence of heterostyly in S. African species ofBauhinia (Leguminosae),Cleome (Capparaceae) andAneilema (Commelinaceae), and inAgelaea (Connaraceae) is discussed.
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    Rumex subgen.Rumex sect.Axillares (Polygonaceae) in South America (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 151-192 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Polygonaceae ; Rumex subg.Rumex sect.Axillares ; Taxonomy ; systematics ; hybridization ; Flora of S. America
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Resumen Rumex secciónAxillares fue descrito por primera vez por el autor en 1937. Se conoce en América del Norte con 20 especies endémicas. De América del Sur se conocían sólo 5 especies, todas ellas endemicas; este número asciende a 15 en la presente revision. Fuera de las Américas se encuentran pequeños grupos de especies endémicas en Sudáfrica, en las Islas de Hawaii y en Australia; especies aisladas se encuentran en Africa Central, en las lejanas Islas Atlánticas de Tristan de Cunha y Gough Island y en Asia Oriental. La sección no tiene representantes en Europa, con excepción de tres especies americanas introducidas, poco frecuentes. La secciónAxillares se caracteriza bien, tanto morfológicamente por su forma de creciemiento—sin rosetas foliares, período floral prácticamente ilimitado por la formación de inflorescencias axilares parciales, flores a menudo monoicas—como por su estructura genética que se manifiesta en un comportamiento diferente de hibridación. Híbridos son frecuentes en las especies de la secciónRumex (syn.Simplices) y reconocibles por su esterilidad que se expresa en cierta particularidad del habito. Al contrario, no hay seguridad de híbridos espontáneos en la secciónAxillares. La mayor parte de los híbridos artificiales son totalmente fértiles. Hasta ahora se conocían solo tres híbridos entre las especies de ambas secciones. Otros tres han sido encontrados por el autor en Chile. Todos completamente estériles y con las características de hábito típicas resultantes la esterilidad, como ocurre en los híbridos de la secciónRumex.
    Notes: Abstract Rumex sect.Axillares first described by the author in 1937 is known to be represented in N. America by 20 endemic species. From S. America only five species, all endemic, were previously known; this number is raised in the present revision to 15. Outside America there exist small groups of endemic species in S. Africa and in the islands of Hawaii as well as in Australia and scattered single species in C. Africa, on the remote Atlantic islands of Tristan de Cunha and Gough Island, and in eastern Asia.—In Europe the section is completely absent, except for three American species which occur as rare aliens. TheAxillares are not only morphologically well-characterized by their growth—no leaf rosettes, flowering period practically unlimited by formation of later flowering axillary shoots, flowers often monoecious—but also by their genetic structure expressed by a different behaviour in hybridization. Hybrids are frequent between species of sect.Rumex (syn.Simplices) and are easy to recognize by their sterility which results in a certain peculiarity in habit. In contrast, no spontaneous hybrids are known with certainty among species of sect.Axillares. Most artificial hybrids are fairly fertile. So far only three hybrids between species of the two sections have been known. Three more have been found by the author in Chile, all being highly sterile and showing the growth characteristics resulting from sterility as occur in hybrids between species of sect.Rumex.
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    Robertsonian change in allies ofZebrina (Commelinaceae) (1990)
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    Plant systematics and evolution 172 (1990), S. 263-271 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Commelinaceae ; Tradescantia soconuscana ; Zebrina ; Chromosome evolution ; Robertsonian change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Meiotic pairing inTradescantia soconuscana 2n=26 (6M+16A+4T) suggests that it has a tetraploid constitution which is not apparent in its chromosome number. Its “nombre fondamental” of 32 indicates that it could have evolved from an ancestor with x=8 by a combination of Robertsonian fusion, hybridization and polyploidy. The 2n=16 (8A+8T) karyotype of a closely related diploid supports this. The allied genusZebrina may have followed a similar method of chromosome evolution.
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    Aspects of floral structure and phenology in the genusConophytum (Mesembryanthemaceae) (1990)
    Springer
    Plant systematics and evolution 172 (1990), S. 229-240 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Mesembryanthemaceae ; Conophytum ; Floral morphology ; floral phenology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The complex floral structure in the southern African genusConophytum (Mesembryanthemaceae; 77 spp.) is described in detail and assigned to three basic floral types, two of which can be divided into two subtypes. Correlations between structural features and phenological patterns are demonstrated and discussed in the family context as well as in relation to the systematic subdivision of the genus.
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    The chemotaxonomy ofGeranium (Geraniaceae) (1990)
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    Plant systematics and evolution 173 (1990), S. 1-15 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Geraniaceae ; Geranium ; Chemotaxonomy ; seed proteins ; essential oils ; nectar amino acids ; additivity in hybrids and allopolyploids ; chemical divergence and mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Relationships amongGeranium species constituting sectt.Anemonifolia, Lucida (monotypic) andRuberta together with representatives of sect.Unguiculata were investigated by gas-chromatographic study of essential oils, electrophoretic comparison of seed proteins and chromatographic separation of nectar amino acids. — Essential oil study gave little information.G. macrorrhizum (sect.Unguiculata) had far greater quantities of essential oils in its foliage than other species and differed from them qualitatively. — Species of sectt.Anemonifolia andRuberta, together withG. cataractarum (sect.Unguiculata), between them yielded 19 seed protein bands; the distribution of these indicated close relationship among the species and was consistent with hypotheses for the origin of certain species by alloand autopolyploidy partly within the group. Involvement of an unknown species in the origin of two allopolyploids was implied. Separate origins for the two octoploid species in this set are also inferred. In two instances there was evidence for the transformation of one band into another subsequent to the separation of related species. The inference of allopolyploidy was supported by the occurrence of additivity of parental bands shown by an artificial hybrid between two of the species. A model for the evolutionary divergence of the seed protein patterns is presented. Two species outside the above set appeared less closely related; they wereG. lucidum andG. macrorrhizum (sect.Unguiculata) and between them they showed 6 additional bands, four of which were shared. — Of 18 nectar amino acids found, 4 to 15 occurred in any one species, with low numbers (4 and 8) occurring in the most extreme inbreeders. The spectra of nectar amino acids of two hybrids showed additivity of those of the respective parents. The results echo rather closely those provided by seed proteins, but in the absence of data from outside the group their taxonomic significance is uncertain. However, the divergence between the two octoploids was again evident.
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    DNA “fingerprints” detect genetic variation inAcer negundo (Aceraceae) (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 49-56 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Aceraceae ; Acer negundo ; box elder ; RFLPs ; minisatellite DNA ; M13 probe ; DNA analysis ; genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic DNA samples from 21 box elder plants collected in Missouri (U.S.A.) were digested with restriction enzyme and southern blot hybridized with the M13 minisatellite probe. Each plant was found to have a unique DNA fragment pattern. Moreover, levels of genetic variation estimated from a similarity index appear to be related to sampling distances. However, size of the fragments utilized in the analysis affects the estimates of genetic variation to a considerable degree.
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    Prolamins in systematics ofPoaceae subfam.Arundinoideae (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 57-70 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Arundinoideae ; Protein ; prolamin ; immunology ; immunoblotting ; electrophoresis ; numerical systematics ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Circumscription of theArundinoideae and the taxonomic treatments of its tribes have long been disputed. In the present study, 21 species representing the tribesArundineae, Danthonieae, Cortaderieae, andAristideae were examined for prolamin size heterogeneity and immunological cross-reactivities. The immunmological data were analyzed phenetically and cladistically. Prolamins of species examined were similar in size and size diversity except forPhragmites. Structural similarities as measured by ELISA and immunoblotting were very high among all genera exceptAristida andPhragmites. Based on prolamin structure,Aristida is not similar to the core genera of theArundinoideae, the remaining genera could not be distinguished as distinct tribes, and theArundinoideae as represented by the taxa tested appeared monophyletic.
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    A numerical taxonomic study of thePolygonum aviculare complex (Polygonaceae) in Belgium (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 71-89 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Polygonaceae ; Polygonum aviculare ; Numerical taxonomy ; weeds ; ruderal strategists ; population variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 16 morphological characters were assessed in 300 plants sampled from 27 populations ofPolygonum aviculare sensu lato representative of the whole ecological range of the species in Belgium. The three multivariate treatments performed, namely principal component analysis, cluster analysis and discriminant analysis provide evidence thatP. aviculare can be divided in 4 units, roughly corresponding to the four taxa recognized byChrtek. However, the results point out that subsp.monspeliense and subsp.calcatum should be included at the varietal rank within respectively subsp.aviculare (=P. heterophyllum) and subsp.aequale (=P. arenastrum) as defined byLindman. Fruit dimensions, fruit shape and tepal length are the most discriminant characters for separating the four taxa, while several other characters are discriminant at the population level only. The evolutionary significance of the variation pattern of the whole complex is discussed in terms of life history differentiation and ecological specialization.
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    URL: http://dx.doi.org/10.1007/BF00937764
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    Flavonoid aglycones from the leaf surfaces of someLabiatae species (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 109-118 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Labiatae ; Exudate flavonoids ; ecological biochemistry ; biochemical systematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of excreted flavonoid aglycones within the familyLabiatae was studied and differences were found, especially in the A-ring substitution patterns. Thus, 5,7-dihydroxy-6-methoxyflavones with substituted B-rings are characteristic of species ofSalvia (sect.Salvia),Rosmarinus andOcimum; 5,7-dihydroxy-6,8-dimethoxyflavones occur only inOcimum and 5,6-dihydroxy-7,8-dimethoxyflavones inThymus and related species. Members of the two subfamiliesLamioideae andNepetoideae produce exudate flavonoids, but some genera are devoid of these compounds. There is a correlation between the habitat where the plant grows and production of these compounds, the species from (semi-)arid habitats being those which generally accumulate external flavonoids.
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    URL: http://dx.doi.org/10.1007/BF00940856
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    Studies on theStellaria longipes complex (Caryophyllaceae): Isozyme variability and the relationship betweenStellaria longipes andS. longifolia (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 129-141 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Caryophyllaceae ; Stellaria longipes ; S. longifolia ; Isozyme variability ; allozyme analysis ; allopolyploidy ; population differentiation ; genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic variation within and the relationship betweenStellaria longipes Goldie andS. longifolia Muhl. were studied. Ten enzyme systems were assessed in eight natural populations ofS. longipes (25 loci) and three ofS. longifolia (20 loci) using starch and polyacrylamide gel electrophoresis. Patterns of population differentiation corresponded to geographic distance. There was no evidence that polyploidS. longipes had greater electrophoretic variability than diploidS. longipes. The isozyme data confirmed extensive population differentiation in these species and, within that context, a relatively close relationship betweenS. longipes andS. longifolia. It was postulated that diploids of these two species might be the progenitors of tetraploidS. longipes.
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    URL: http://dx.doi.org/10.1007/BF00940858
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    Pollination of New CaledonianWinteraceae: Opportunistic shifts or parallel radiation with their pollinators? (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 143-157 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Winteraceae ; Zygogynum ; Lepidoptera ; Micropterigidae ; Sabatinca ; Coleoptera ; Pollination biology ; coevolution ; floral volatiles ; parallel radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Flower visitors on 12 species of New CaledonianWinteraceae were studied. The visitors were two species of ancestral moths (Sabatinca; Micropterigidae), three species of weevils (Palontus; Curculionidae), and a species of thrips. Behavior observations and pollen records suggest that the beetles and occasionally the moths serve as pollinators ofZygogynum and Exospermum, andBelliolum is pollinated primarily by thrips. The floral volatiles are simple in composition, usually dominated by short esters. Preliminary experiments showed that ethyl acetate elicited alighting, and a distinctive huddling behavior was elicited by artificially mixed fragrance. The host associations of otherSabatinca andPalontus spp. do not support the hypothesis that theWinteraceae have radiated in association with their pollinators. The available evidence supports the notion of opportunistic isolated host colonizations at some point in the radiation of the pollinator groups.
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    URL: http://dx.doi.org/10.1007/BF00940859
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  • 95
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    Nuclear DNA variability withinPisum sativum (Leguminosae): Cytophotometric analyses (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 179-185 
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    ISSN: 1615-6110
    Keywords: Angiosperms ; Leguminosae ; Pisum sativum ; Cytophotometry ; nuclear DNA content ; DNA variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cultivars or experimental lines ofPisum sativum were analyzed cytophotometrically for nuclear DNA content of early prophases after ethanol-acetic acid fixation. Wide variability was found (from 3.93 to 5.07pg per haploid nucleus). This result was confirmed by the cytophotometric analysis of interphase nuclei isolated from leaf tissues fixed in formalin. Analysis of interphase nuclei at different thresholds of optical density showed that certain chromatin fractions are involved in the variations.
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    URL: http://dx.doi.org/10.1007/BF00940861
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    The pollen morphology ofOdontites (Scrophulariaceae) and its taxonomic significance (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 159-178 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Scrophulariaceae ; Odontites ; Odontitella ; Macrosyringion ; Bornmuellerantha ; Omphalothrix ; Euphrasia ; Bartsia ; Parentucellia ; Pollen morphology ; exine sculpturing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The pollen morphology of 29 species ofOdontites and related genera was examined by light and scanning electron microscopy. Three major pollen types differing fundamentally in exine sculpturing were found. In the parasitic tribePedicularieae retipilate sculpturing is the most widespread type representing a plesiomorphic character state from which the two other types are derived. Of these, reticulate sculpturing is confined toOdontites, whereas a complex retirugulate pattern was found only in the monotypic Near East genusBornmuellerantha. Within the retipilate and the reticulate major types eight minor types were distinguished based on the differential correlation of exine surface morphology, size, shape and amb form. The pollen data are generally well correlated with macromorphological features and furnish important taxonomic characters at the genus—as well as at the specieslevel.
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    URL: http://dx.doi.org/10.1007/BF00940860
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    Ribosomal RNA genes structure in somePopulus spp. (Salicaceae) and their hybrids (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 187-196 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Salicaceae ; Populus ; Ribosomal RNA genes ; restriction enzyme maps ; rDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The tandemly repeated multigene families encoding 18S and 25S rRNAs were studied at the restriction enzyme level inPopulus alba L.,Populus deltoides Bartr. exMarsh.,Populus trichocarpa Torr. & Gray and in the hybrids between the last two mentioned species. The analysis of single and double digestion with EcoRI, BamHI, XbaI, and SstI endonucleases showed the presence of single repetitive unit types of 12.25 and 11.75kb inP. alba andP. trichocarpa, respectively.P. deltoides showed two rDNA gene types having the same length (12.25Kb) but different nucleotide sequence in the IGS. The rDNAs genes ofP. deltoides andP. triochocarpa are inherited codominantly in their hybrids.
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    URL: http://dx.doi.org/10.1007/BF00940862
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    Morphological and anatomical differentiation ofVellozia hirsuta populations (Velloziaceae) (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 197-208 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Velloziaceae ; Vellozia hirsuta ; Morphological and anatomical variation ; geographical differentiation ; taxonomy ; Flora of the campos rupestres ; Brazil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphological and leaf anatomical differentiation ofVellozia hirsuta is analysed and classified into several types (A1, A2, A4, B3, B5, C3). The species has a relatively wide distribution in the campos rupestres of Minas Gerais in Brazil. The variation of the isolated populations on different mountain ranges is complex, does not follow a clear geographical pattern, and defies taxonomic classification.
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    URL: http://dx.doi.org/10.1007/BF00940863
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    The production of floral oils byMonttea (Scrophulariaceae) and the function of tarsal pads inCentris bees (1990)
    Springer
    Plant systematics and evolution 173 (1990), S. 209-222 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Scrophulariaceae ; Monttea ; Hymenoptera ; Anthophoridae ; Centris ; Oil-flowers ; oil-bees ; Flora of Argentina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant species that secrete oil as their primary floral reward are rare and sporadically found in the angiosperms. We report here thatMonttea, a genus previously unsuspected of being an oil-plant, produces lipids from trichome elaiophores on the inside of the lower (anterior) lip. The discovery of the production of oils by species of this S. American genus explains the occurrence of unusual dual-function collecting structures in ArgentineCentris (Hymenoptera: Anthophoridae) and explains the presence of oil-collecting bees in regions where oil-secreting flowers were previously thought to be absent. The behavior of these centridine pollinators onMonttea flowers parallels that of oil-collecting bees onDiascia (Scrophulariaceae) in S. Africa.
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    URL: http://dx.doi.org/10.1007/BF00940864
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    Isolation and characterization of the membrane-bound cytochrome c-554 from the thermophilic green photosynthetic bacterium Chloroflexus aurantiacus (1990)
    Springer
    Photosynthesis research 23 (1990), S. 29-38 
    Details
    ISSN: 1573-5079
    Keywords: cytochrome c ; photosynthesis ; photosynthetic bacteria ; electron transport ; Chloroflexus aurantiacus ; green bacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The membrane-bound photooxidizable cytochrome c-554 from Chloroflexus aurantiacus has been purified. The purified protein runs as a single heme staining band on SDS-PAGE with an apparent molecular mass of 43 000 daltons. An extinction coefficient of 28 ± 1 mM−1 cm−1 per heme at 554 nm was found for the dithionite-reduced protein. The potentiometric titration of the hemes takes place over an extended range, showing clearly that the protein does not contain a single heme in a well-defined site. The titration can be fit to a Nernst curve with midpoint potentials at 0, +120, +220 and +300 mV vs the standard hydrogen electrode. Pyridine hemochrome analysis combined with a Lowry protein assay and the SDS-PAGE molecular weight indicates that there are a minimum of three, and probably four hemes per peptide. Amino acid analysis shows 5 histidine residues and 29% hydrophobic residues in the protein. This cytochrome appears to be functionally similar to the bound cytochrome from Rhodopseudomonas viridis. Both cytochrome c-554 from C. aurantiacus and the four-heme cytochrome c-558-553 from R. viridis appear to act as direct electron donors to the special bacteriochlorophyll pair of the photosynthetic reaction center. They have a similar content of hydrophobic amino acids, but differ in isoelectric point, thermodynamic characteristics, spectral properties, and in their ability to be photooxidized at low temperature.
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    URL: http://dx.doi.org/10.1007/BF00030060
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